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Sample material was fractionated with an aqueous (MilliQ) solution of sodium polytungstate (SPT; Na6[H2W12O40]), with a density of 1.8 g cm\u00b3, followed by wet-sieving over a 63 \u00b5m mesh, thus separating loose OC from mineral-associated OC. Each fraction was analysed for element content (TOC, TN), carbon isotopes (\u03b4\u00b9\u00b3C, \u0394\u00b9\u2074C), molecular biomarkers (n-alkanes, n-alkanoic acids, lignin phenols, cutin acids), and mineral surface area. The relative abundance of specific biomarkers can also be used as indicator for degradation of organic carbon. Furthermore, the OC 'loading' (concentration of OC normalised to mineral surface area; in mgOC/m\u00b2) and terrestrial biomarker loading (\u00b5gOC/m\u00b2) can be used to assess loss of (permafrost) OC from mineral particles. 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We collected water samples for measuring water chemistry, and we determined landscape parameters using GIS based tools. The data was collected at three sampling periods in summer 2021 in the Zackenberg Research Station (74\u00b028'N, 20\u00b034'W). The area has a polar tundra climate with mean annual air temperature of -9.1 \u00b0C. Water chemistry (i.e. dissolved and particulate nitrogen, phosphorus, carbon; dissolved iron and silicate) and catchment characteristics (i.e. catchment area, altitude, slope, aspect, NDVI, snow cover) was measured for each of the 14 stream sites. Water chemistry samples were collected and analyzed using standard methods, and landscape characteristics were determined using GIS resources. The data was collected in order to study relationships between landscape characteristics and stream water chemistry. 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