Legume roots can be symbiotically colonized by arbuscular mycorrhizal (AM) fungi and nitrogen-fixing bacteria. In Lotus japonicus, the latter occurs intracellularly by the cognate rhizobial partner Mesorhizobium loti or intercellularly with the Agrobacterium pusense strain IRBG74. Although these symbiotic programs show distinctive cellular and transcriptome signatures, some molecular components are shared. In this study, we demonstrate that 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase 1 (DAHPS1), the first enzyme in the biosynthetic pathway of aromatic amino acids (AAAs), plays a critical role in root hair development and for AM and rhizobial symbioses in Lotus. Two homozygous DAHPS1 mutants (dahps1-1 and dahps1-2) showed drastic alterations in root hair morphology, associated with alterations in cell wall dynamics and a progressive disruption of the actin cytoskeleton. The altered root hair structure was prevented by pharmacological and genetic complementation. dahps1-1 and dahps1-2 showed significant reductions in rhizobial infection (intracellular and intercellular) and nodule organogenesis and a delay in AM colonization. RNAseq analysis of dahps1-2 roots suggested that these phenotypes are associated with downregulation of several cell wall\uffe2\uff80\uff93related genes, and with an attenuated signaling response. Interestingly, the dahps1 mutants showed no detectable pleiotropic effects, suggesting a more selective recruitment of this gene in certain biological processes. This work provides robust evidence linking AAA metabolism to root hair development and successful symbiotic associations.Ecosystems often show differential sensitivity to chronic nitrogen (N) deposition; hence, a critical challenge is to improve our understanding of how and why site\uffe2\uff80\uff90specific factors mediate biogeochemical responses to N enrichment. We examined the extent to which N impacts on soil carbon (C) and N dynamics depend on microbial resource stoichiometry. We added N to forest plots dominated by ectomycorrhizal (ECM) trees, which have litter and soil pools rich in organic N and relatively wide C:N ratios, and adjacent forest plots dominated by arbuscular mycorrhizal (AM) trees, which have litter and soil pools rich in inorganic N and relatively narrow C:N ratios. While microbes in both plot types exhibited fairly strict biomass homeostasis, microbes in AM\uffe2\uff80\uff90 and ECM\uffe2\uff80\uff90dominated plots differed in their physiological responses to N addition. Microbes in ECM plots responded to N enrichment by decreasing their investment in N\uffe2\uff80\uff90acquisition enzymes (relative to C\uffe2\uff80\uff90acquisition enzymes) and increasing N mineralization rates (relative to C mineralization rates), suggesting that N addition alleviated microbial N demand. In contrast, heterotrophic microbial activities in AM plots were unaffected by N addition, most likely as a result of N\uffe2\uff80\uff90induced increases in net nitrification (60% increase relative to control plots) and nitrate mobilization (e.g., sixfold increases in mobilization relative to control plots). Combined, our findings suggest the stoichiometric differences between AM and ECM soils are the primary drivers of the observed responses. Plant and microbial communities characterized by wide C:N are more susceptible to N\uffe2\uff80\uff90induced changes in decomposition and soil C dynamics, whereas communities characterized by narrow C:N are more susceptible to N\uffe2\uff80\uff90induced nitrate leaching losses. Hence, the biogeochemical consequences of N deposition in temperate forests may be driven by the stoichiometry of the dominant trees and their associated microbes.
", "keywords": ["2. Zero hunger", "Soil", "Nitrates", "Nitrogen", "13. Climate action", "Mycorrhizae", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Forests", "Nitrogen Cycle", "15. Life on land", "Nitrification"]}, "links": [{"href": "https://doi.org/10.1002/ecy.1595"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecy.1595", "name": "item", "description": "10.1002/ecy.1595", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecy.1595"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-12-01T00:00:00Z"}}, {"id": "10.1002/etc.5400", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:09Z", "type": "Journal Article", "created": "2022-06-09", "title": "Arbuscular Mycorrhizal Fungi and the Need for a Meaningful Regulatory Plant Protection Product Testing Strategy", "description": "AbstractArbuscular mycorrhizal fungi (AMF) perform key soil ecosystem services and, because of their symbiotic relationship with plant roots, may be exposed to the plant protection products (PPPs) applied to soils and crops. In 2017, the European Food Safety Authority (EFSA) released a scientific opinion addressing the state of the science on risk assessment of PPPs for in-soil organisms, recommending the inclusion of AMF ecotoxicological testing in the PPP regulatory process. However, it is not clear how this can be implemented in a tiered, robust, and ecologically relevant manner. Through a critical review of current literature, we examine the recommendations made within the EFSA report and the methodologies available to integrate AMF into the PPP risk assessment and provide perspective and commentary on their agronomic and ecological relevance. We conclude that considerable research questions remain to be addressed prior to the inclusion of AMF into the in-soil organism risk assessment, many of which stem from the unique challenges associated with including an obligate symbiont within the PPP risk assessment. Finally, we highlight critical knowledge gaps and the further research required to enable development of relevant, reliable, and robust scientific tests alongside pragmatic and scientifically sound guidance to ensure that any future risk-assessment paradigm is adequately protective of the ecosystem services it aims to preserve. Environ Toxicol Chem 2022;41:1808\uffe2\uff80\uff931823. \uffc2\uffa9 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.Better cover crop management options aiming to maximize the benefits of arbuscular mycorrhizal fungi (AMF) to subsequent crops are largely unknown. We investigated the impact of cover crop management methods on maize growth performance and assemblages of AMF colonizing maize roots in a field trial. The cover crop treatments comprised Italian ryegrass, wheat, brown mustard and fallow in rotation with maize.
RESULTSThe diversity of AMF communities among cover crops used for maize management was significantly influenced by the cover crop and time course. Cover crops did not affect grain yield and aboveground biomass of subsequent maize but affected early growth. A structural equation model indicated that the root colonization, AMF diversity and maize phosphorus uptake had direct strong positive effects on yield performance.
CONCLUSIONAMF variables and maize performance were related directly or indirectly to maize grain yield, whereas root colonization had a positive effect on maize performance. AMF may be an essential factor that determines the success of cover crop rotational systems. Encouraging AMF associations can potentially benefit cover cropping systems. Therefore, it is imperative to consider AMF associations and crop phenology when making management decisions. \uffc2\uffa9 2017 Society of Chemical Industry
", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "0402 animal and dairy science", "Agriculture", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "Zea mays", "Crop Production", "03 medical and health sciences", "Mycorrhizae", "Lolium", "0405 other agricultural sciences", "Soil Microbiology", "Triticum", "Mustard Plant"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.8606"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.8606", "name": "item", "description": "10.1002/jsfa.8606", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.8606"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-09-11T00:00:00Z"}}, {"id": "10.1007/s00248-008-9390-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:30Z", "type": "Journal Article", "created": "2008-04-28", "title": "Plant Responses To Drought Stress And Exogenous Aba Application Are Modulated Differently By Mycorrhization In Tomato And An Aba-Deficient Mutant (Sitiens)", "description": "The aims of the present study are to find out whether the effects of arbuscular mycorrhizal (AM) symbiosis on plant resistance to water deficit are mediated by the endogenous abscisic acid (ABA) content of the host plant and whether the exogenous ABA application modifies such effects. The ABA-deficient tomato mutant sitiens and its near-isogenic wild-type parental line were used. Plant development, physiology, and expression of plant genes expected to be modulated by AM symbiosis, drought, and ABA were studied. Results showed that only wild-type tomato plants responded positively to mycorrhizal inoculation, while AM symbiosis was not observed to have any effect on plant development in sitiens plants grown under well-watered conditions. The application of ABA to sitiens plants enhanced plant growth both under well-watered and drought stress conditions. In respect to sitiens plants subjected to drought stress, the addition of ABA had a cumulative effect in relation to that of inoculation with G. intraradices. Most of the genes analyzed in this study showed different regulation patterns in wild-type and sitiens plants, suggesting that their gene expression is modulated by the plant ABA phenotype. In the same way, the colonization of roots with the AM fungus G. intraradices differently regulated the expression of these genes in wild-type and in sitiens plants, which could explain the distinctive effect of the symbiosis on each plant ABA phenotype. This also suggests that the effects of the AM symbiosis on plant responses and resistance to water deficit are mediated by the plant ABA phenotype.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "15. Life on land", "Adaptation", " Physiological", "Plant Roots", "6. Clean water", "Droughts", "03 medical and health sciences", "Solanum lycopersicum", "Mycorrhizae", "Mutation", "Symbiosis", "Abscisic Acid"]}, "links": [{"href": "https://doi.org/10.1007/s00248-008-9390-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-008-9390-y", "name": "item", "description": "10.1007/s00248-008-9390-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-008-9390-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-04-29T00:00:00Z"}}, {"id": "10.1007/s00374-006-0152-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:34Z", "type": "Journal Article", "created": "2006-11-27", "title": "Biological Nitrogen Fixation By Common Beans (Phaseolus Vulgaris L.) Increases With Bio-Char Additions", "description": "This study examines the potential, magnitude, and causes of enhanced biological N2 fixation (BNF) by common beans (Phaseolus vulgaris L.) through bio-char additions (charcoal, biomass-derived black carbon). Bio-char was added at 0, 30, 60, and 90 g kg\u22121 soil, and BNF was determined using the isotope dilution method after adding 15N-enriched ammonium sulfate to a Typic Haplustox cropped to a potentially nodulating bean variety (CIAT BAT 477) in comparison to its non-nodulating isoline (BAT 477NN), both inoculated with effective Rhizobium strains. The proportion of fixed N increased from 50% without bio-char additions to 72% with 90 g kg\u22121 bio-char added. While total N derived from the atmosphere (NdfA) significantly increased by 49 and 78% with 30 and 60 g kg\u22121 bio-char added to soil, respectively, NdfA decreased to 30% above the control with 90 g kg\u22121 due to low total biomass production and N uptake. The primary reason for the higher BNF with bio-char additions was the greater B and Mo availability, whereas greater K, Ca, and P availability, as well as higher pH and lower N availability and Al saturation, may have contributed to a lesser extent. Enhanced mycorrhizal infections of roots were not found to contribute to better nutrient uptake and BNF. Bean yield increased by 46% and biomass production by 39% over the control at 90 and 60 g kg\u22121 bio-char, respectively. However, biomass production and total N uptake decreased when bio-char applications were increased to 90 g kg\u22121. Soil N uptake by N-fixing beans decreased by 14, 17, and 50% when 30, 60, and 90 g kg\u22121 bio-char were added to soil, whereas the C/N ratios increased from 16 to 23.7, 28, and 35, respectively. Results demonstrate the potential of bio-char applications to improve N input into agroecosystems while pointing out the needs for long-term field studies to better understand the effects of bio-char on BNF.", "keywords": ["2. Zero hunger", "fijaci\u00f3n biol\u00f3gica del nitr\u00f3geno", "phaseolus vulgaris", "04 agricultural and veterinary sciences", "biological nitrogen fixation", "15. Life on land", "suelo \u00e1cido", "7. Clean energy", "01 natural sciences", "acid soils", "6. Clean water", "rhizobium", "0401 agriculture", " forestry", " and fisheries", "mycorrhizae", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Juan Ram\u00edrez, Johannes Lehmann, Mar\u00eda del Pilar Hurtado, Marco Antonio Rond\u00f3n, Marco Antonio Rond\u00f3n,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s00374-006-0152-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00374-006-0152-z", "name": "item", "description": "10.1007/s00374-006-0152-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00374-006-0152-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-11-24T00:00:00Z"}}, {"id": "10.1007/s00442-006-0562-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:40Z", "type": "Journal Article", "created": "2006-10-10", "title": "Is Microbial Community Composition In Boreal Forest Soils Determined By Ph, C-To-N Ratio, The Trees, Or All Three?", "description": "In Fennoscandian boreal forests, soil pH and N supply generally increase downhill as a result of water transport of base cations and N, respectively. Simultaneously, forest productivity increases, the understory changes from ericaceous dwarf shrubs to tall herbs; in the soil, fungi decrease whereas bacteria increase. The composition of the soil microbial community is mainly thought to be controlled by the pH and C-to-N ratio of the substrate. However, the latter also determines the N supply to plants, the plant community composition, and should also affect plant allocation of C below ground to roots and a major functional group of microbes, mycorrhizal fungi. We used phospholipid fatty acids (PLFAs) to analyze the potential importance of mycorrhizal fungi by comparing the microbial community composition in a tree-girdling experiment, where tree belowground C allocation was terminated, and in a long-term (34 years) N loading experiment, with the shifts across a natural pH and N supply gradient. Both tree girdling and N loading caused a decline of ca. 45% of the fungal biomarker PLFA 18:2omega6,9, suggesting a common mechanism, i.e., that N loading caused a decrease in the C supply to ectomycorrhizal fungi just as tree girdling did. The total abundance of bacterial PLFAs did not respond to tree girdling or to N loading, in which cases the pH (of the mor layer) did not change appreciably, but bacterial PLFAs increased considerably when pH increased across the natural gradient. Fungal biomass was high only in acid soil (pH < 4.1) with a high C-to-N ratio (>38). According to a principal component analysis, the soil C-to-N ratio was as good as predictor of microbial community structure as pH. Our study thus indicated the soil C-to-N ratio, and the response of trees to this ratio, as important factors that together with soil pH influence soil microbial community composition.", "keywords": ["Sweden", "Soil", "Bacteria", "Nitrogen", "Mycorrhizae", "Fatty Acids", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "15. Life on land", "Carbon", "Soil Microbiology", "Trees"]}, "links": [{"href": "https://doi.org/10.1007/s00442-006-0562-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-006-0562-5", "name": "item", "description": "10.1007/s00442-006-0562-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-006-0562-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-10-11T00:00:00Z"}}, {"id": "10.1007/s00442-012-2471-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:41Z", "type": "Journal Article", "created": "2012-10-03", "title": "Ectomycorrhizal Fungal Succession Coincides With Shifts In Organic Nitrogen Availability And Canopy Closure In Post-Wildfire Jack Pine Forests", "description": "Successional changes in belowground ectomycorrhizal fungal (EMF) communities have been observed with increasing forest stand age; however, mechanisms behind this change remain unclear. It has been hypothesized that declines of inorganic nitrogen (N) and increases of organic N influence changes in EMF taxa over forest development. In a post-wildfire chronosequence of six jack pine (Pinus banksiana) stands ranging in age from 5 to 56 years, we investigated EMF community composition and compared shifts in taxa with detailed soluble inorganic and organic N data. Taxa were identified by internal transcribed spacer rDNA sequencing, and changes in community composition evaluated with non-metric multi-dimensional scaling (NMDS). Dissimilarities in the community data were tested for correlations with N variables. We observed a successional shift along NMDS axis 1 from such taxa as Suillus brevipes and Thelephora terrestris in sites age 5 and 11 to species of Cortinarius and Russula, among others, in the four older sites. This change was positively correlated with soluble organic N (SON) (r(2) = 0.902, P = 0.033) and free amino-acid N (r(2) = 0.945, P = 0.021), but not inorganic N. Overall, our results show a successional shift of EMF communities occurring between stand initiation and canopy closure without a change in species of the dominant plant-host, and associated with SON and free amino-acid N in soil. It is uncertain whether EMF taxa are responding to these organic N forms directly, affecting their availability, or are ultimately responding to changes in other site variables, such as belowground productivity.", "keywords": ["0106 biological sciences", "Mycorrhizae", "DNA", " Ribosomal Spacer", "Population Dynamics", "0401 agriculture", " forestry", " and fisheries", "Biodiversity", "04 agricultural and veterinary sciences", "Nitrogen Cycle", "15. Life on land", "Pinus", "01 natural sciences", "Fires"]}, "links": [{"href": "https://doi.org/10.1007/s00442-012-2471-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-012-2471-0", "name": "item", "description": "10.1007/s00442-012-2471-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-012-2471-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-10-04T00:00:00Z"}}, {"id": "10.1007/s00572-015-0627-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:42Z", "type": "Journal Article", "created": "2015-02-13", "title": "Nitrogen and carbon/nitrogen dynamics in arbuscular mycorrhiza: the great unknown", "description": "Many studies have established that arbuscular mycorrhizal fungi transfer N to the host plant. However, the role and importance of arbuscular mycorrhiza (AM) in plant N nutrition is still uncertain, as are the C/N interactions within the symbiosis. Published reports provide differing, and often contradictory, results that are difficult to combine in a coherent framework. This review explores questions such as: What makes the difference between a positive and a negative effect of AM on plant N nutrition? Is the mycorrhizal N response (MNR) correlated to the mycorrhizal growth response (MGR), and how or under which conditions? Is the MNR effect on plant growth C mediated? Is plant C investment on fungal growth related to N needs or N benefit? How is the N for C trade between symbionts regulated? The patternless nature of current knowledge is made evident, and possible reasons for this are discussed.", "keywords": ["2. Zero hunger", "Nitrogen", "Mycorrhizae", "Plant Development", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Plants", "Symbiosis", "Carbon"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00572-015-0627-6"}, {"href": "https://doi.org/10.1007/s00572-015-0627-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00572-015-0627-6", "name": "item", "description": "10.1007/s00572-015-0627-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00572-015-0627-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-02-14T00:00:00Z"}}, {"id": "10.1007/s00572-016-0694-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:42Z", "type": "Journal Article", "created": "2016-04-14", "title": "Organic Amendments Increase Phylogenetic Diversity Of Arbuscular Mycorrhizal Fungi In Acid Soil Contaminated By Trace Elements", "description": "In 1998, a toxic mine spill polluted a 55-km(2) area in a basin southward to Do\u00f1ana National Park (Spain). Subsequent attempts to restore those trace element-contaminated soils have involved physical, chemical, or biological methodologies. In this study, the restoration approach included application of different types and doses of organic amendments: biosolid compost (BC) and leonardite (LEO). Twelve years after the last addition, molecular analyses of arbuscular mycorrhizal (AM) fungal communities associated with target plants (Lamarckia aurea and Chrysanthemum coronarium) as well as analyses of trace element concentrations both in soil and in plants were performed. The results showed an improved soil quality reflected by an increase in soil pH and a decrease in trace element availability as a result of the amendments and dosages. Additionally, the phylogenetic diversity of the AM fungal community increased, reaching the maximum diversity at the highest dose of BC. Trace element concentration was considered the predominant soil factor determining the AM fungal community composition. Thereby, the studied AM fungal community reflects a community adapted to different levels of contamination as a result of the amendments. The study highlights the long-term effect of the amendments in stabilizing the soil system.", "keywords": ["2. Zero hunger", "0301 basic medicine", "Minerals", "0303 health sciences", "Bioindicator", "Chrysanthemum", "Genetic Variation", "Hydrogen-Ion Concentration", "15. Life on land", "Poaceae", "Soil biodiversity", "Trace element contaminated soils", "Ecosystem restoration", "Mining", "Soil fungal community", "Trace Elements", "Soil", "03 medical and health sciences", "Biodegradation", " Environmental", "13. Climate action", "Mycorrhizae", "Mine spill", "Bioindicators", "Soil Pollutants", "Phylogeny"]}, "links": [{"href": "https://doi.org/10.1007/s00572-016-0694-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00572-016-0694-3", "name": "item", "description": "10.1007/s00572-016-0694-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00572-016-0694-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-04-12T00:00:00Z"}}, {"id": "10.1007/s00572-015-0655-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:42Z", "type": "Journal Article", "created": "2015-07-25", "title": "The Ectomycorrhizal Community Of Conifer Stands On Peat Soils 12 Years After Fertilization With Wood Ash", "description": "We studied long-term effects of fertilization with wood ash on biomass, vitality and mycorrhizal colonization of fine roots in three conifer forest stands growing in Vacciniosa turf. mel. (V), Myrtillosa turf. mel. (M) and Myrtillosa turf. mel./Caricoso-phragmitosa (MC) forest types on peat soils. Fertilization trials amounting 5 kg/m(2) of wood ash were established 12 years prior to this study. A total of 63 soil samples with roots were collected and analysed. Ectomycorrhizal (ECM) fungi in roots were identified by morphotyping and sequencing of the fungal internal transcribed spacer (ITS) region. In all forest types, fine root biomass was higher in fertilized plots than in control plots. In M forest type, proportion of living fine roots was greater in fertilized plots than in control plots, while in V and MC, the result was opposite. Fifty ECM species were identified, of which eight were common to both fertilized and control plots. Species richness and Shannon diversity index were generally higher in fertilized plots than in control plots. The most common species in fertilized plots were Amphinema byssoides (17.8%) and Tuber cf. anniae (12.2%), while in control plots, it was Tylospora asterophora (18.5%) and Lactarius tabidus (20.3%). Our results showed that forest fertilization with wood ash has long-lasting effect on diversity and composition of ECM fungal communities.", "keywords": ["0106 biological sciences", "570", "forest fertilization", "m\u00e4nty", "Molecular Sequence Data", "organic soils", "fine roots", "Plant Roots", "01 natural sciences", "630", "mets\u00e4nlannoitus", "Mycorrhizae", "ectomycorrhizae", "DNA", " Ribosomal Spacer", "Muut aihealueet", "DNA", " Fungal", "2. Zero hunger", "Picea abies", "Pinus sylvestris", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "15. Life on land", "Biota", "hienojuuret", "kuusi", "Tracheophyta", "eloper\u00e4iset maat", "0401 agriculture", " forestry", " and fisheries", "ektomykorritsa"]}, "links": [{"href": "https://doi.org/10.1007/s00572-015-0655-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00572-015-0655-2", "name": "item", "description": "10.1007/s00572-015-0655-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00572-015-0655-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-07-26T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2015.06.044", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:16:05Z", "type": "Journal Article", "created": "2015-07-02", "title": "Contrasting Effects Of Biochar Versus Manure On Soil Microbial Communities And Enzyme Activities In An Aridisol", "description": "Biochar can increase microbial activity, alter microbial community structure, and increase soil fertility in arid and semi-arid soils, but at relatively high rates that may be impractical for large-scale field studies. This contrasts with organic amendments such as manure, which can be abundant and inexpensive if locally available, and thus can be applied to fields at greater rates than biochar. In a field study comparing biochar and manure, a fast pyrolysis hardwood biochar (22.4 Mg ha(-1)), dairy manure (42 Mg ha(-1) dry wt), a combination of biochar and manure at the aforementioned rates, or no amendment (control) was applied to an Aridisol (n=3) in fall 2008. Plots were annually cropped to corn (Zea maize L.). Surface soils (0-30 cm) were sampled directly under corn plants in late June 2009 and early August 2012, and assayed for microbial community fatty acid methyl ester (FAME) profiles and six extracellular enzyme activities involved in soil C, N, and P cycling. Arbuscular mycorrhizal (AM) fungal colonization was assayed in corn roots in 2012. Biochar had no effect on microbial biomass, community structure, extracellular enzyme activities, or AM fungi root colonization of corn. In the short-term, manure amendment increased microbial biomass, altered microbial community structure, and significantly reduced the relative concentration of the AM fungal biomass in soil. Manure also reduced the percent root colonization of corn by AM fungi in the longer-term. Thus, biochar and manure had contrasting short-term effects on soil microbial communities, perhaps because of the relatively low application rate of biochar.", "keywords": ["2. Zero hunger", "04 agricultural and veterinary sciences", "15. Life on land", "Zea mays", "6. Clean water", "Enzymes", "Manure", "Chemistry", "Soil", "Fertility", "Charcoal", "Mycorrhizae", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Extracellular Space", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2015.06.044"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2015.06.044", "name": "item", "description": "10.1016/j.chemosphere.2015.06.044", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2015.06.044"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2008.06.038", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:16:19Z", "type": "Journal Article", "created": "2008-08-16", "title": "Bioconcentration Of Zinc And Cadmium In Ectomycorrhizal Fungi And Associated Aspen Trees As Affected By Level Of Pollution", "description": "Concentrations of Zn and Cd were measured in fruitbodies of ectomycorrhizal (ECM) fungi and leaves of co-occurring accumulator aspen. Samples were taken on three metal-polluted sites and one control site. Fungal bioconcentration factors (BCF = fruitbody concentration: soil concentration) were calculated on the basis of total metal concentrations in surface soil horizons (BCF(tot)) and NH(4)NO(3)-extractable metal concentrations in mineral soil (BCF(lab)). When plotted on log-log scale, values of BCF decreased linearly with increasing soil metal concentrations. BCF(lab) for both Zn and Cd described the data more closely than BCF(tot). Fungal genera differed in ZnBCF but not in CdBCF. The information on differences between fungi with respect to their predominant occurrence in different soil horizons did not improve relations of BCF with soil metal concentrations. Aspen trees accumulated Zn and Cd to similar concentrations as the ECM fungi. Apparently, the fungi did not act as an effective barrier against aspen metal uptake by retaining the metals.", "keywords": ["Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Trees", "Zinc", "13. Climate action", "Mycorrhizae", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Environmental Pollution", "Cadmium", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2008.06.038"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2008.06.038", "name": "item", "description": "10.1016/j.envpol.2008.06.038", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2008.06.038"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-01-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2010.04.017", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:16:20Z", "type": "Journal Article", "created": "2010-05-25", "title": "Molecular Diversity Of Arbuscular Mycorrhizal Fungi In Relation To Soil Chemical Properties And Heavy Metal Contamination", "description": "Abundance and diversity of arbuscular mycorrhizal fungi (AMF) associated with dominant plant species were studied along a transect from highly lead (Pb) and zinc (Zn) polluted to non-polluted soil at the Anguran open pit mine in Iran. Using an established primer set for AMF in the internal transcribed spacer (ITS) region of rDNA, nine different AMF sequence types were distinguished after phylogenetic analyses, showing remarkable differences in their distribution patterns along the transect. With decreasing Pb and Zn concentration, the number of AMF sequence types increased, however one sequence type was only found in the highly contaminated area. Multivariate statistical analysis revealed that further factors than HM soil concentration affect the AMF community at contaminated sites. Specifically, the soils' calcium carbonate equivalent and available P proved to be of importance, which illustrates that field studies on AMF distribution should also consider important environmental factors and their possible interactions.", "keywords": ["0301 basic medicine", "Soil", "Zinc", "03 medical and health sciences", "Lead", "13. Climate action", "Mycorrhizae", "Soil Pollutants", "Biodiversity", "15. Life on land", "01 natural sciences", "Phylogeny", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2010.04.017"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2010.04.017", "name": "item", "description": "10.1016/j.envpol.2010.04.017", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2010.04.017"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-01T00:00:00Z"}}, {"id": "10.1111/nph.15230", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:48Z", "type": "Journal Article", "created": "2018-05-28", "title": "Partner communication and role of nutrients in the arbuscular mycorrhizal symbiosis", "description": "Contents Summary 1031 I. Introduction 1031 II. Interkingdom communication enabling symbiosis 1032 III. Nutritional and regulatory roles for key metabolites in the AM symbiosis 1035 IV. The plant\uffe2\uff80\uff93fungus genotype combination determines the outcome of the symbiosis 1039 V. Perspectives 1039 Acknowledgements 1041 References 1041
SummaryThe evolutionary and ecological success of the arbuscular mycorrhizal (AM) symbiosis relies on an efficient and multifactorial communication system for partner recognition, and on a fine\uffe2\uff80\uff90tuned and reciprocal metabolic regulation of each symbiont to reach an optimal functional integration. Besides strigolactones, N\uffe2\uff80\uff90acetylglucosamine\uffe2\uff80\uff90derivatives released by the plant were recently suggested to trigger fungal reprogramming at the pre\uffe2\uff80\uff90contact stage. Remarkably, N\uffe2\uff80\uff90acetylglucosamine\uffe2\uff80\uff90based diffusible molecules also are symbiotic signals produced by AM fungi (AMF) and clues on the mechanisms of their perception by the plant are emerging. AMF genomes and transcriptomes contain a battery of putative effector genes that may have conserved and AMF\uffe2\uff80\uff90 or host plant\uffe2\uff80\uff90specific functions. Nutrient exchange is the key feature of AM symbiosis. A mechanism of phosphate transport inside fungal hyphae has been suggested, and first insights into the regulatory mechanisms of root colonization in accordance with nutrient transfer and status were obtained. The recent discovery of the dependency of AMF on fatty acid transfer from the host has offered a convincing explanation for their obligate biotrophism. Novel studies highlighted the importance of plant and fungal genotypes for the outcome of the symbiosis. These findings open new perspectives for fundamental research and application of AMF in agriculture.
", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Nitrogen", "Phosphorus", "Plants", "15. Life on land", "symbiosis", "lipids", "03 medical and health sciences", "nutrients", "Mycorrhizae", "arbuscular mycorrhizal fungi (AMF)", "Metabolome", "natural variation", "signalling", "Symbiosis", "effectors", "phosphate"]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/1667502/1/Pre-print%20IRIS_%20review%20New%20Phytol%202018.pdf"}, {"href": "https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.15230"}, {"href": "https://doi.org/10.1111/nph.15230"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.15230", "name": "item", "description": "10.1111/nph.15230", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.15230"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-28T00:00:00Z"}}, {"id": "10.1016/j.plantsci.2017.06.006", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:17:00Z", "type": "Journal Article", "created": "2017-06-27", "title": "Belowground microbes mitigate plant-plant competition", "description": "Dimorphandra wilsonii, a Cerrado endemic Fabaceae tree, is threatened by land-use changes. The few remaining individuals occur in areas dominated by alien grasses like Urochloa decumbens. We tested the impact of nitrogen (N) availability and symbionts' presence on mitigating the effects of competition from U. decumbens. Dimorphandra wilsonii seedlings were 50-week pot-cultivated under limiting (3mM) or non-limiting (10mM)\u2009N, with or without U. decumbens, and inoculated or not with a N-fixer (Bradyrhizobium sp.) and an arbuscular mycorrhizal fungus (AMF - Glomus etunicatum), both forming symbioses in the field. Since D. wilsonii seedlings grew more and 'lost' fewer nutrients under the symbionts' presence, symbionts mitigated plant-plant competition. Under limiting N, inoculated D. wilsonii seedlings grew more (despite no nodulation), but N fixation was only suggested when inoculated D. wilsonii seedlings competed with U. decumbens. D. wilsonii13C, and substrate's carbon and respiration suggest that only the microbes performing key functions received plant carbon. Under non-limiting N, inoculated D. wilsonii seedlings became enriched in 13C, substrate accumulated carbon and microbial respiration increased, suggesting a more generalist microbial community. Data suggest inoculating D. wilsonii seeds/seedlings with AMF and N-fixers as a conservation measure. However, long-term field-studies need to confirm these conclusions.", "keywords": ["2. Zero hunger", "0301 basic medicine", "03 medical and health sciences", "Nitrogen", "Mycorrhizae", "0401 agriculture", " forestry", " and fisheries", "Fabaceae", "Bradyrhizobium", "04 agricultural and veterinary sciences", "15. Life on land", "Symbiosis", "3. Good health"]}, "links": [{"href": "https://doi.org/10.1016/j.plantsci.2017.06.006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.plantsci.2017.06.006", "name": "item", "description": "10.1016/j.plantsci.2017.06.006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.plantsci.2017.06.006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-09-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2019.01.095", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:17:07Z", "type": "Journal Article", "created": "2019-01-10", "title": "The Cover Crop Determines The Amf Community Composition In Soil And In Roots Of Maize After A Ten-Year Continuous Crop Rotation", "description": "Intensive agricultural practices are responsible for soil biological degradation. By stimulating indigenous arbuscular mycorrhizal fungi (AMF), cover cropping enhances soil health and promotes agroecosystem sustainability. Still, the legacy effects of cover crops (CCs) and the major factors driving the AM fungal community are not well known; neither is the influence of the specific CC. This work describes a field experiment established in Central Spain to test the effect of replacing winter fallow by barley (Hordeum vulgare L.) or vetch (Vicia sativa L.) during the intercropping of maize (Zea mays L.). We examined the community composition of the AMF in the roots and rhizosphere soil associated with the subsequent cash crop after 10\u202fyears of cover cropping, using Illumina technology. The multivariate analysis showed that the AMF communities under the barley treatment differed significantly from those under fallow, whereas no legacy effect of the vetch CC was detected. Soil organic carbon, electrical conductivity, pH, Ca and microbial biomass carbon were identified as major factors shaping soil AMF communities. Specific AMF taxa were found to play a role in plant uptake of P, Fe, Zn, Mn, and Cd, which may shed light on the functionality of these taxa. In our conditions, the use of barley as a winter CC appears to be an appropriate choice with respect to promotion of AMF populations and biological activity in agricultural soils with intercropping systems. However, more research on CC species and their legacy effect on the microbial community composition and functionality are needed to guide decisions in knowledge-based agriculture.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Diversity", "Cover cropping", "Grass", "Arbuscular mycorrhizal fungi", "Agriculture", "Hordeum", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "Zea mays", "Crop Production", "Legume", "Spain", "Long-term experiment", "Mycorrhizae", "Long-term experiments", "Rhizosphere", "0401 agriculture", " forestry", " and fisheries", "Illumina technology", "Mediterranean climate", "Soil Microbiology", "Mycobiome"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2019.01.095"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2019.01.095", "name": "item", "description": "10.1016/j.scitotenv.2019.01.095", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2019.01.095"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.175008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:17:10Z", "type": "Journal Article", "created": "2024-07-23", "title": "Mycorrhizal association controls soil carbon-degrading enzyme activities and soil carbon dynamics under nitrogen addition: A systematic review", "description": "Recent evidence suggests that changes in carbon-degrading extracellular enzyme activities (C-EEAs) can help explain soil organic carbon (SOC) dynamics under nitrogen (N) addition. However, the factors controlling C-EEAs remain unclear, impeding the inclusion of microbial mechanisms in global C cycle models. Using meta-analysis, we show that the responses of C-EEAs to N addition were best explained by mycorrhizal association across a wide range of environmental and experimental factors. In ectomycorrhizal (ECM) dominated ecosystems, N addition suppressed C-EEAs targeting the decomposition of structurally complex macromolecules by 13.1\u00a0%, and increased SOC stocks by 5.2\u00a0%. In contrast, N addition did not affect C-EEAs and SOC stocks in arbuscular mycorrhizal (AM) dominated ecosystems. Our results indicate that earlier studies may have overestimated SOC changes under N addition in AM-dominated ecosystems and underestimated SOC changes in ECM-dominated ecosystems. Incorporating this mycorrhizal-dependent impact of EEAs on SOC dynamics into Earth system models could improve predictions of SOC dynamics under environmental changes.", "keywords": ["Free-living decomposers", "2. Zero hunger", "Soil organic carbon", "Nitrogen", "Nitrogen availability", "15. Life on land", "Carbon", "Carbon Cycle", "Soil", "13. Climate action", "Mycorrhizae", "Soil extracellular enzyme", "Mycorrhizal fungi", "Soil Microbiology", "Ecosystem"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2024.175008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.175008", "name": "item", "description": "10.1016/j.scitotenv.2024.175008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.175008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-01T00:00:00Z"}}, {"id": "10.1371/journal.pbio.3002127", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:20:13Z", "type": "Journal Article", "created": "2023-05-18", "title": "A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus", "description": "Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception.Inter-kingdom belowground carbon (C) transfer is a significant, yet hidden, biological phenomenon, due to the complexity and highly dynamic nature of soil ecology. Among key biotic agents influencing C allocation belowground are ectomycorrhizal fungi (EMF). EMF symbiosis can extend beyond the single tree-fungus partnership to form common mycorrhizal networks (CMNs). Despite the high prevalence of CMNs in forests, little is known about the identity of the EMF transferring the C and how these in turn affect the dynamics of C transfer. Here, Pinus halepensis and Quercus calliprinos saplings growing in forest soil were labeled using a 13CO2 labeling system. Repeated samplings were applied during 36 days to trace how 13C was distributed along the tree-fungus-tree pathway. To identify the fungal species active in the transfer, mycorrhizal fine root tips were used for DNA-stable isotope probing (SIP) with 13CO2 followed by sequencing of labeled DNA. Assimilated 13CO2 reached tree roots within four days and was then transferred to various EMF species. C was transferred across all four tree species combinations. While Tomentella ellisii was the primary fungal mediator between pines and oaks, Terfezia pini, Pustularia spp., and Tuber oligospermum controlled C transfer among pines. We demonstrate at a high temporal, quantitative, and taxonomic resolution, that C from EMF host trees moved into EMF and that C was transferred further to neighboring trees of similar and distinct phylogenies.
", "keywords": ["0301 basic medicine", "Quercus", "Soil", "0303 health sciences", "03 medical and health sciences", "Mycorrhizae", "Carbon Dioxide", "15. Life on land", "Plant Roots", "Carbon", "Trees"]}, "links": [{"href": "https://www.nature.com/articles/s41396-022-01193-z.pdf"}, {"href": "https://doi.org/10.1038/s41396-022-01193-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41396-022-01193-z", "name": "item", "description": "10.1038/s41396-022-01193-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41396-022-01193-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-18T00:00:00Z"}}, {"id": "10.1038/s42003-022-04178-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:18Z", "type": "Journal Article", "created": "2022-11-17", "title": "Both abundant and rare fungi colonizing Fagus sylvatica ectomycorrhizal root-tips shape associated bacterial communities", "description": "AbstractEctomycorrhizal fungi live in close association with their host plants and form complex interactions with bacterial/archaeal communities in soil. We investigated whether abundant or rare ectomycorrhizal fungi on root-tips of young beech trees (Fagus sylvatica) shape bacterial/archaeal communities. We sequenced 16S rRNA genes and fungal internal transcribed spacer regions of individual root-tips and used ecological networks to detect the tendency of certain assemblies of fungal and bacterial/archaeal taxa to inhabit the same root-tip (i.e. modularity). Individual ectomycorrhizal root-tips hosted distinct fungal communities associated with unique bacterial/archaeal communities. The structure of the fungal-bacterial/archaeal association was determined by both, dominant and rare fungi. Integrating our data in a conceptual framework suggests that the effect of rare fungi on the bacterial/archaeal communities of ectomycorrhizal root-tips contributes to assemblages of bacteria/archaea on root-tips. This highlights the potential impact of complex fine-scale interactions between root-tip associated fungi and other soil microorganisms for the ectomycorrhizal symbiosis.Litter quality parameters of Danthonia richardsonii grown under CO2 concentrations of \uffe2\uff89\uff88\uffe2\uff80\uff83359 & \uffe2\uff89\uff88\uffe2\uff80\uff83719\uffe2\uff80\uff83\uffce\uffbcL L\uffe2\uff88\uff92\uffe2\uff80\uff8a1 at three mineral N supply rates (2.2, 6.7 & 19.8\uffe2\uff80\uff83g\uffe2\uff80\uff83m\uffe2\uff88\uff92\uffe2\uff80\uff8a2\uffe2\uff80\uff83y\uffe2\uff88\uff92\uffe2\uff80\uff8a1) were determined. C:N ratio was increased in senesced leaf (enhancement ratios, Re/c, of 1.25\uffe2\uff80\uff931.67), surface litter (1.34\uffe2\uff80\uff931.64) and root (1.13\uffe2\uff80\uff931.30) by CO2 enrichment. After 3\uffe2\uff80\uff83years of growth, nonstructural carbohydrate concentrations were reduced in senesced leaf lamina (avg. Re/c=\uffe2\uff80\uff8a\uffe2\uff80\uff830.84) but not in root in response to CO2 enrichment. Cellulose concentrations increased slightly in senesced leaf (avg. Re/c=\uffe2\uff80\uff8a\uffe2\uff80\uff831.07) but not in root in response to CO2 enrichment. Lignin and polyphenolic concentrations in senesced leaf and root were not changed by CO2 enrichment. Decomposition, measured as cumulative respiration in standard conditions in vitro, was reduced in leaf litter grown under CO2 enrichment. Root decomposition in vitro was lower in the material produced under CO2 enrichment at the two higher rates of mineral N supply. Significant correlations between decomposition of leaf litter and initial %N, C:N ratio and lignin:N ratio were found. Decomposition in vivo, measured as carbon disappearance from the surface litter was not affected by CO2 concentration. Arbuscular mycorrhizal infection was not changed by CO2 enrichment. Microbial carbon was higher under CO2 enrichment at the two higher rates of mineral N supply. Possible reasons for the lack of effect of changes in litter quality on in\uffe2\uff80\uff90sward decomposition rates are discussed.
", "keywords": ["decomposition", "grass", "Arbuscular mycorrhizae", "Microbial biomass", "carbon dioxide", "04 agricultural and veterinary sciences", "15. Life on land", "nitrogen", "microcosm", "C3 plant", "litter", "Danthonia", "biochemical composition", "Long-term experiment", "Keywords: arbuscular mycorrhiza", "Climate change", "0401 agriculture", " forestry", " and fisheries", "nutrient availability", "Danthonia richardsonii C:N"], "contacts": [{"organization": "Jason L. Lutze, Jason L. Lutze, Roger M. Gifford, Helen N. Adams,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1046/j.1365-2486.2000.00277.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1046/j.1365-2486.2000.00277.x", "name": "item", "description": "10.1046/j.1365-2486.2000.00277.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1046/j.1365-2486.2000.00277.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2000-01-01T00:00:00Z"}}, {"id": "10.1080/01904167.2015.1087032", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:46Z", "type": "Journal Article", "created": "2015-09-12", "title": "Phosphorus Fertilization Of A Grass-Legume Mixture: Effect On Plant Growth, Nutrients Acquisition And Symbiotic Associations With Soil Microorganisms", "description": "Fil: Mendoza, Rodolfo Ernesto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales 'bernardino Rivadavia'; Argentina", "keywords": ["2. Zero hunger", "0106 biological sciences", "https://purl.org/becyt/ford/4.5", "Arbuscular Mycorrhizae-Rhizobia-Dark Septate Endophyte", "0401 agriculture", " forestry", " and fisheries", "https://purl.org/becyt/ford/4", "04 agricultural and veterinary sciences", "Lotus-Festuca Mix", "15. Life on land", "N-P Limited Environment", "01 natural sciences"], "contacts": [{"organization": "Mendoza, Rodolfo Ernesto, Bailleres, Mat\u00edas Andres, Garc\u00eda, Ileana Vanesa, Ruiz, Oscar Adolfo,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1080/01904167.2015.1087032"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Plant%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1080/01904167.2015.1087032", "name": "item", "description": "10.1080/01904167.2015.1087032", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1080/01904167.2015.1087032"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-09-11T00:00:00Z"}}, {"id": "10.1093/femsec/fiw024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:57Z", "type": "Journal Article", "created": "2016-02-06", "title": "Mycorrhizal Fungal Communities Respond To Experimental Elevation Of Soil Ph And P Availability In Temperate Hardwood Forests", "description": "Many forests are affected by chronic acid deposition, which can lower soil pH and limit the availability of nutrients such as phosphorus (P), but the response of mycorrhizal fungi to changes in soil pH and P availability and how this affects tree acquisition of nutrients is not well understood. Here, we describe an ecosystem-level manipulation in 72 plots, which increased pH and/or P availability across six forests in Ohio, USA. Two years after treatment initiation, mycorrhizal fungi on roots were examined with molecular techniques, including 454-pyrosequencing. Elevating pH significantly increased arbuscular mycorrhizal (AM) fungal colonization and total fungal biomass, and affected community structure of AM and ectomycorrhizal (EcM) fungi, suggesting that raising soil pH altered both mycorrhizal fungal communities and fungal growth. AM fungal taxa were generally negatively correlated with recalcitrant P pools and soil enzyme activity, whereas EcM fungal taxa displayed variable responses, suggesting that these groups respond differently to P availability. Additionally, the production of extracellular phosphatase enzymes in soil decreased under elevated pH, suggesting a shift in functional activity of soil microbes with pH alteration. Thus, our findings suggest that elevating pH increased soil P availability, which may partly underlie the mycorrhizal fungal responses we observed.", "keywords": ["Fungi", "Phosphorus", "04 agricultural and veterinary sciences", "Forests", "Hydrogen-Ion Concentration", "15. Life on land", "Plant Roots", "Trees", "Soil", "Mycorrhizae", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1093/femsec/fiw024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiw024", "name": "item", "description": "10.1093/femsec/fiw024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiw024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-04T00:00:00Z"}}, {"id": "10.1093/femsec/fiz133", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:57Z", "type": "Journal Article", "created": "2019-08-22", "title": "Restriction of plant roots in boreal forest organic soils affects the microbial community but does not change the dominance from ectomycorrhizal to saprotrophic fungi", "description": "ABSTRACTBoreal forest soils store significant amounts of carbon and are cohabited by saprotrophic and ectomycorrhizal fungi (ECM). The \uffe2\uff80\uff98Gadgil effect\uffe2\uff80\uff99 implies antagonistic interactions between saprotrophic fungi and ECM. Plant photosynthates support the competitive fitness of the ECM, and may also shape the soil bacterial communities. Many \uffe2\uff80\uff98Gadgil effect\uffe2\uff80\uff99 experiments have focused on litter layer (OL) or have litter and root-fragments present, and thus possibly favor the saprotrophs. We compared how the restriction of plant roots and exudates affect soil microbial community structures in organic soil (mixed OF and OH). For this, we established a 3-yr field experiment with 3 different mesh treatments affecting the penetration of plant roots and external fungal hyphae. Exclusion of plant photosynthates induced modest changes in both fungal and bacterial community structures, but not to potential functionality of the microbial community. The microbial community was resilient towards rather short-term disturbances. Contrary to the \uffe2\uff80\uff98Gadgil effect\uffe2\uff80\uff99, mesh treatments restricting the entrance of plant roots and external fungal hyphae did not favor saprotrophs that originally inhabited the soil. Thus, we propose that different substrate preferences (fresh litter vs. fermented or humified soil), rather than antagonism, maintain the spatial separation of saprotrophs and mycorrhizal fungi in boreal forest soils.
", "keywords": ["0301 basic medicine", "570", "Hyphae", "577", "Plant Roots", "ectomycorrhiza", "Trees", "Soil", "03 medical and health sciences", "boreal forest soil", "Mycorrhizae", "Taiga", "saprotrophs", "Soil Microbiology", "2. Zero hunger", "0303 health sciences", "Microbiota", "Fungi", "Plants", "15. Life on land", "Gadgil effect", "Carbon", "functional gene profile", "13. Climate action", "ta1181", "microbial community"]}, "links": [{"href": "http://academic.oup.com/femsec/article-pdf/95/9/fiz133/29808832/fiz133.pdf"}, {"href": "https://doi.org/10.1093/femsec/fiz133"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiz133", "name": "item", "description": "10.1093/femsec/fiz133", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiz133"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-23T00:00:00Z"}}, {"id": "10.1093/treephys/tpu116", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:02Z", "type": "Journal Article", "created": "2015-01-24", "title": "Dinitrogen Fixation By Legume Shade Trees And Direct Transfer Of Fixed N To Associated Cacao In A Tropical Agroforestry System", "description": "Natural abundance of (15)N (\u03b4\u2009(15)N) was determined in bulk soil, rhizospheric soil and vegetation in an organically managed cacao (Theobroma cacao L.) plantation with Inga edulis Mart. legume trees (inga) as the principal shade for studying the nitrogen (N) cycle in the system. Cacao without contact with legumes in an adjacent plantation was used as the reference for N2 fixation and direct N transfer calculations. Bulk and rhizospheric soils contained 72 and 20%, respectively, of whole- system N. No vegetation effect on \u03b4\u2009(15)N in rhizospheric soil was detected, probably due to the high native soil N pool. Fine roots of the cacaos associated with inga contained \u223c35% of N fixed from the atmosphere (Nf) out of the total N. Leaves of all species had significantly higher \u03b4\u2009(15)N than fine roots. Twenty percent of system Nf was found in cacao suggesting direct N transfer from inga via a common mycelial network of mycorrhizal fungi or recycling of N-rich root exudates of inga. Inga had accumulated 98\u2005kg [Nf] ha(-1) during the 14-year history of the plantation. The conservative estimate of current N2 fixation rate was 41\u2005kg [Nf] ha(-1)\u2005year(-1) based on inga biomass only and 50\u2005kg [Nf] ha(-1)\u2005year(-1) based on inga and associated trees.", "keywords": ["2. Zero hunger", "Cacao", "Tropical Climate", "Nitrogen Isotopes", "Nitrogen", "Plant Exudates", "Fabaceae", "04 agricultural and veterinary sciences", "Forests", "15. Life on land", "Plant Roots", "Trees", "Plant Leaves", "Soil", "Mycorrhizae", "Nitrogen Fixation", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1093/treephys/tpu116"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Tree%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/treephys/tpu116", "name": "item", "description": "10.1093/treephys/tpu116", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/treephys/tpu116"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-24T00:00:00Z"}}, {"id": "10.1111/gcb.15277", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:23Z", "type": "Journal Article", "created": "2020-07-12", "title": "Low phosphorus supply constrains plant responses to elevated CO 2 : A meta\u2010analysis", "description": "AbstractPhosphorus (P) is an essential macro\uffe2\uff80\uff90nutrient required for plant metabolism and growth. Low P availability could potentially limit plant responses to elevated carbon dioxide (eCO2), but consensus has yet to be reached on the extent of this limitation. Here, based on data from experiments that manipulated both CO2 and P for young individuals of woody and non\uffe2\uff80\uff90woody species, we present a meta\uffe2\uff80\uff90analysis of P limitation impacts on plant growth, physiological, and morphological response to eCO2. We show that low P availability attenuated plant photosynthetic response to eCO2 by approximately one\uffe2\uff80\uff90quarter, leading to a reduced, but still positive photosynthetic response to eCO2 compared to those under high P availability. Furthermore, low P limited plant aboveground, belowground, and total biomass responses to eCO2, by 14.7%, 14.3%, and 12.4%, respectively, equivalent to an approximate halving of the eCO2 responses observed under high P availability. In comparison, low P availability did not significantly alter the eCO2\uffe2\uff80\uff90induced changes in plant tissue nutrient concentration, suggesting tissue nutrient flexibility is an important mechanism allowing biomass response to eCO2 under low P availability. Low P significantly reduced the eCO2\uffe2\uff80\uff90induced increase in leaf area by 14.3%, mirroring the aboveground biomass response, but low P did not affect the eCO2\uffe2\uff80\uff90induced increase in root length. Woody plants exhibited stronger attenuation effect of low P on aboveground biomass response to eCO2 than non\uffe2\uff80\uff90woody plants, while plants with different mycorrhizal associations showed similar responses to low P and eCO2 interaction. This meta\uffe2\uff80\uff90analysis highlights crucial data gaps in capturing plant responses to eCO2 and low P availability. Field\uffe2\uff80\uff90based experiments with longer\uffe2\uff80\uff90term exposure of both CO2 and P manipulations are critically needed to provide ecosystem\uffe2\uff80\uff90scale understanding. Taken together, our results provide a quantitative baseline to constrain model\uffe2\uff80\uff90based hypotheses of plant responses to eCO2 under P limitation, thereby improving projections of future global change impacts.
", "keywords": ["0106 biological sciences", "910", "01 natural sciences", "XXXXXX - Unknown", "soil phosphorus", "Humans", "phosphorus", "Photosynthesis", "mycorrhizae", "soils", "Ecosystem", "0105 earth and related environmental sciences", "580", "nutrient concentration", "2. Zero hunger", "plant morphology", "biomass", "plants", "carbon dioxide", "Phosphorus", "mycorrhizas", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Plants", "15. Life on land", "leaf gas exchange", "meta-analysis", "plant nutrient uptake", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.15277"}, {"href": "https://doi.org/10.1111/gcb.15277"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.15277", "name": "item", "description": "10.1111/gcb.15277", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.15277"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-31T00:00:00Z"}}, {"id": "10.1111/gcb.16135", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:23Z", "type": "Journal Article", "created": "2022-02-17", "title": "Mycorrhizal fungi alleviate acidification\u2010induced phosphorus limitation: Evidence from a decade\u2010long field experiment of simulated acid deposition in a tropical forest in south China", "description": "AbstractSouth China has been experiencing very high rate of acid deposition and severe soil acidification in recent decades, which has been proposed to exacerbate the regional ecosystem phosphorus (P) limitation. We conducted a 10\uffe2\uff80\uff90year field experiment of simulated acid deposition to examine how acidification impacts seasonal changes of different soil P fractions in a tropical forest with highly acidic soils in south China. As expected, acid addition significantly increased occluded P pool but reduced the other more labile P pools in the dry season. In the wet season, however, acid addition did not change microbial P, soluble P and labile organic P pools. Acid addition significantly increased exchangeable Al3+ and Fe3+ and the activation of Fe oxides in both seasons. Different from the decline of microbial abundance in the dry season, acid addition increased ectomycorrhizal fungi and its ratio to arbuscular mycorrhiza fungi in the wet season, which significantly stimulated phosphomonoesterase activities and likely promoted the dissolution of occluded P. Our results suggest that, even in already highly acidic soils, the acidification\uffe2\uff80\uff90induced P limitation could be alleviated by stimulating ectomycorrhizal fungi and phosphomonoesterase activities. The differential responses and microbial controls of seasonal soil P transformation revealed here should be implemented into ecosystem biogeochemical model for predicting plant productivity under future acid deposition scenarios.
Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore\uffe2\uff80\uff90space, and models of AMF\uffe2\uff80\uff90enhanced P\uffe2\uff80\uff90uptake are poorly validated.
We used synchrotron X\uffe2\uff80\uff90ray computed tomography to visualize mycorrhizas in soil and synchrotron X\uffe2\uff80\uff90ray fluorescence/X\uffe2\uff80\uff90ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling.
We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co\uffe2\uff80\uff90locate with areas of high P and low Al, and preferentially associate with organic\uffe2\uff80\uff90type P species over Al\uffe2\uff80\uff90rich inorganic P.
We discovered that AMF avoid Al\uffe2\uff80\uff90rich areas as a source of P. Sulphur\uffe2\uff80\uff90rich regions were found to be correlated with higher hyphal density and an increased organic\uffe2\uff80\uff90associated P\uffe2\uff80\uff90pool, whilst oxidized S\uffe2\uff80\uff90species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome\uffe2\uff80\uff90related. Our experimentally\uffe2\uff80\uff90validated model led to an estimate of P\uffe2\uff80\uff90uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated \uffe2\uff80\uff93 a result with significant implications for the modelling of plant\uffe2\uff80\uff93soil\uffe2\uff80\uff93AMF interactions.
", "keywords": ["580", "X-ray computed tomography", "570", "Research", "X-ray fluorescence", "Fungi", "Hyphae", "500", "Phosphorus", "mycorrhizas", "04 agricultural and veterinary sciences", "15. Life on land", "plant phosphorus uptake", "Plant Roots", "Soil", "rhizosphere modelling", "Mycorrhizae", "synchrotron", "0401 agriculture", " forestry", " and fisheries", "Soil Microbiology"]}, "links": [{"href": "https://eprints.soton.ac.uk/454110/1/Keyes_et_al_Myco_Paper_TR_04_01_2022_unmarked.pdf"}, {"href": "https://eprints.soton.ac.uk/454110/2/Figures_TR_22_12_2021.pdf"}, {"href": "https://eprints.soton.ac.uk/454110/3/SI_1_TR_22_12_2021_no_markup.pdf"}, {"href": "https://eprints.soton.ac.uk/454110/4/SI_2_TR_22_12_2021.pdf"}, {"href": "https://doi.org/10.17863/cam.81466"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.17863/cam.81466", "name": "item", "description": "10.17863/cam.81466", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.17863/cam.81466"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-15T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2007.02150.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:39Z", "type": "Journal Article", "created": "2007-07-10", "title": "Decline Of Arbuscular Mycorrhizal Fungi In Northern Hardwood Forests Exposed To Chronic Nitrogen Additions", "description": "Arbuscular mycorrhizal (AM) fungi are important below-ground carbon (C) sinks that can be sensitive to increased nitrogen (N) availability. The abundance of AM fungi (AMF) was estimated in maple (Acer spp.) fine roots following more than a decade of experimental additions of N designed to simulate chronic atmospheric N deposition. Abundance of AMF was measured by staining and ocular estimation, as well as by analyzing for the AMF indicator fatty acid 16:1omega5c in phospholipid (biomass indicator) and neutral lipid (lipid storage indicator) fractions. Arbuscular mycorrhizal fungal biomass, storage structures and lipid storage declined in response to N addition measured by both methods. This pattern was found when AM response was characterized as colonization intensity, on an areal basis and in proportion to maple above-ground biomass. The phospholipid fraction of the fatty acid 16:1omega5c was positively correlated with total AMF colonization and the neutral lipid fraction with vesicle colonization. Decreased AMF abundance with simulated N deposition suggests reduced C allocation to these fungi or a direct soil N-mediated decline. The fatty acid (phospholipid and neutral lipid fractions) 16:1omega5c was found to be a good indicator for AMF active biomass and stored energy, respectively.", "keywords": ["Michigan", "Nitrogen", "Mycorrhizae", "0401 agriculture", " forestry", " and fisheries", "Acer", "Biomass", "04 agricultural and veterinary sciences", "15. Life on land", "Lipid Metabolism", "Plant Roots", "Carbon", "Trees"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2007.02150.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2007.02150.x", "name": "item", "description": "10.1111/j.1469-8137.2007.02150.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2007.02150.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-13T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2010.03321.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:40Z", "type": "Journal Article", "created": "2010-06-11", "title": "Belowground Carbon Allocation By Trees Drives Seasonal Patterns Of Extracellular Enzyme Activities By Altering Microbial Community Composition In A Beech Forest Soil", "description": "*Plant seasonal cycles alter carbon (C) and nitrogen (N) availability for soil microbes, which may affect microbial community composition and thus feed back on microbial decomposition of soil organic material and plant N availability. The temporal dynamics of these plant-soil interactions are, however, unclear. *Here, we experimentally manipulated the C and N availability in a beech forest through N fertilization or tree girdling and conducted a detailed analysis of the seasonal pattern of microbial community composition and decomposition processes over 2 yr. *We found a strong relationship between microbial community composition and enzyme activities over the seasonal course. Phenoloxidase and peroxidase activities were highest during late summer, whereas cellulase and protease peaked in late autumn. Girdling, and thus loss of mycorrhiza, resulted in an increase in soil organic matter-degrading enzymes and a decrease in cellulase and protease activity. *Temporal changes in enzyme activities suggest a switch of the main substrate for decomposition between summer (soil organic matter) and autumn (plant litter). Our results indicate that ectomycorrhizal fungi are possibly involved in autumn cellulase and protease activity. Our study shows that, through belowground C allocation, trees significantly alter soil microbial communities, which may affect seasonal patterns of decomposition processes.", "keywords": ["Nitrogen", "Climate", "Trees", "Soil", "Mycorrhizae", "401902 Soil science", "Fagus", "Biomass", "Phospholipids", "Soil Microbiology", "106022 Mikrobiologie", "Bacteria", "Research", "Temperature", "04 agricultural and veterinary sciences", "15. Life on land", "401902 Bodenkunde", "Carbon", "Solubility", "106030 Pflanzen\u00f6kologie", "106022 Microbiology", "Regression Analysis", "0401 agriculture", " forestry", " and fisheries", "106030 Plant ecology", "Seasons", "Extracellular Space", "Biomarkers"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2010.03321.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2010.03321.x", "name": "item", "description": "10.1111/j.1469-8137.2010.03321.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2010.03321.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-07-19T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2011.03776.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:40Z", "type": "Journal Article", "created": "2011-06-08", "title": "Seven Years Of Carbon Dioxide Enrichment, Nitrogen Fertilization And Plant Diversity Influence Arbuscular Mycorrhizal Fungi In A Grassland Ecosystem", "description": "\u2022 We tested the prediction that the abundance and diversity of arbuscular mycorrhizal (AM) fungi are influenced by resource availability and plant community composition by examining the joint effects of carbon dioxide (CO(2) ) enrichment, nitrogen (N) fertilization and plant diversity on AM fungi. \u2022 We quantified AM fungal spores and extramatrical hyphae in 176 plots after 7 yr of treatment with all combinations of ambient or elevated CO(2) (368 or 560 ppm), with or without N fertilization (0 or 4 g Nm(-2) ), and one (monoculture) or 16 host plant species (polyculture) in the BioCON field experiment at Cedar Creek Ecosystem Science Reserve, Minnesota, USA. \u2022 Extramatrical hyphal lengths were increased by CO(2) enrichment, whereas AM spore abundance decreased with N fertilization. Spore abundance, morphotype richness and extramatrical hyphal lengths were all greater in monoculture plots. A structural equation model showed AM fungal biovolume was most influenced by CO(2) enrichment, plant community composition and plant richness, whereas spore richness was most influenced by fungal biovolume, plant community composition and plant richness. \u2022 Arbuscular mycorrhizal fungi responded to differences in host community and resource availability, suggesting that mycorrhizal functions, such as carbon sequestration and soil stability, will be affected by global change.", "keywords": ["0106 biological sciences", "Nitrogen", "Minnesota", "Hyphae", "Poaceae", "Models", " Biological", "01 natural sciences", "nitrogen", "Soil", "Mycorrhizae", "Biomass", "Fertilizers", "Ecosystem", "Soil Microbiology", "vesicular-arbuscular mycorrhizas", "580", "2. Zero hunger", "grassland ecology", "grasslands", "carbon dioxide", "Biodiversity", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Spores", " Fungal", "15. Life on land", "plant diversity", "0401 agriculture", " forestry", " and fisheries", "Plant Shoots"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2011.03776.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2011.03776.x", "name": "item", "description": "10.1111/j.1469-8137.2011.03776.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2011.03776.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-06-08T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2011.03927.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:40Z", "type": "Journal Article", "created": "2011-10-11", "title": "Regional And Local Patterns Of Ectomycorrhizal Fungal Diversity And Community Structure Along An Altitudinal Gradient In The Hyrcanian Forests Of Northern Iran", "description": "Summary
Altitudinal gradients strongly affect the diversity of plants and animals, yet little is known about the altitudinal effects on the distribution of microorganisms, including ectomycorrhizal fungi.
By combining morphological and molecular identification methods, we addressed the relative effects of altitude, temperature, precipitation, host community and soil nutrient concentrations on species richness and community composition of ectomycorrhizal fungi in one of the last remaining temperate old\uffe2\uff80\uff90growth forests in Eurasia.
Molecular analyses revealed 367 species of ectomycorrhizal fungi along three altitudinal transects. Species richness declined monotonically with increasing altitude. Host species and altitude were the main drivers of the ectomycorrhizal fungal community composition at both the local and regional scales. The mean annual temperature and precipitation were strongly correlated with altitude and accounted for the observed patterns of richness and community.
The decline of ectomycorrhizal fungal richness with increasing altitude is consistent with the general altitudinal richness patterns of macroorganisms. Low environmental energy reduces the competitive ability of rare species and thus has a negative effect on the richness of ectomycorrhizal fungi. Because of multicollinearity with altitude, the direct effects of climatic variables and their seasonality warrant further investigation at the regional and continental scales.
", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "Geography", "Altitude", "Climate", "Molecular Sequence Data", "Biodiversity", "Iran", "15. Life on land", "Models", " Biological", "01 natural sciences", "Trees", "Soil", "03 medical and health sciences", "Species Specificity", "13. Climate action", "Mycorrhizae", "Least-Squares Analysis"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2011.03927.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2011.03927.x", "name": "item", "description": "10.1111/j.1469-8137.2011.03927.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2011.03927.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-10-11T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2012.04050.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:40Z", "type": "Journal Article", "created": "2012-01-31", "title": "Direct And Indirect Influences Of 8 Yr Of Nitrogen And Phosphorus Fertilization On Glomeromycota In An Alpine Meadow Ecosystem", "description": "We measured the influences of soil fertility and plant community composition on Glomeromycota, and tested the prediction of the functional equilibrium hypothesis that increased availability of soil resources will reduce the abundance of arbuscular mycorrhizal (AM) fungi. Communities of plants and AM fungi were measured in mixed roots and in Elymus nutans roots across an experimental fertilization gradient in an alpine meadow on the Tibetan Plateau. As predicted, fertilization reduced the abundance of Glomeromycota as well as the species richness of plants and AM fungi. The response of the glomeromycotan community was strongly linked to the plant community shift towards dominance by Elymus nutans. A reduction in the extraradical hyphae of AM fungi was associated with both the changes in soil factors and shifts in the plant community composition that were caused by fertilization. Our findings highlight the importance of soil fertility in regulating both plant and glomeromycotan communities, and emphasize that high fertilizer inputs can reduce the biodiversity of plants and AM fungi, and influence the sustainability of ecosystems.", "keywords": ["2. Zero hunger", "Elymus", "Nitrogen", "Hyphae", "Phosphorus", "Biodiversity", "04 agricultural and veterinary sciences", "Spores", " Fungal", "15. Life on land", "Tibet", "Soil", "Species Specificity", "13. Climate action", "Mycorrhizae", "Linear Models", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Glomeromycota", "Ecosystem"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2012.04050.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2012.04050.x", "name": "item", "description": "10.1111/j.1469-8137.2012.04050.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2012.04050.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-01-31T00:00:00Z"}}, {"id": "10.1111/mec.15351", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:47Z", "type": "Journal Article", "created": "2020-01-10", "title": "Share the wealth: Trees with greater ectomycorrhizal species overlap share more carbon", "description": "AbstractThe mutualistic symbiosis between forest trees and ectomycorrhizal fungi (EMF) is among the most ubiquitous and successful interactions in terrestrial ecosystems. Specific species of EMF are known to colonize specific tree species, benefitting from their carbon source, and in turn, improving their access to soil water and nutrients. EMF also form extensive mycelial networks that can link multiple root\uffe2\uff80\uff90tips of different trees. Yet the number of tree species connected by such mycelial networks, and the traffic of material across them, are just now under study. Recently we reported substantial belowground carbon transfer between Picea, Pinus, Larix and Fagus trees in a mature forest. Here, we analyze the EMF community of these same individual trees and identify the most likely taxa responsible for the observed carbon transfer. Among the nearly 1,200 EMF root\uffe2\uff80\uff90tips examined, 50%\uffe2\uff80\uff9370% belong to operational taxonomic units (OTUs) that were associated with three or four tree host species, and 90% of all OTUs were associated with at least two tree species. Sporocarp 13C signals indicated that carbon originating from labelled Picea trees was transferred among trees through EMF networks. Interestingly, phylogenetically more closely related tree species exhibited more similar EMF communities and exchanged more carbon. Our results show that belowground carbon transfer is well orchestrated by the evolution of EMFs and tree symbiosis.
", "keywords": ["Mycorrhizae", "15. Life on land", "Plant Roots", "Carbon", "Ecosystem", "Trees"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.15351"}, {"href": "https://doi.org/10.1111/mec.15351"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Molecular%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/mec.15351", "name": "item", "description": "10.1111/mec.15351", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/mec.15351"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-27T00:00:00Z"}}, {"id": "10.1111/nph.14083", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:47Z", "type": "Journal Article", "created": "2016-07-11", "title": "Increased Phosphate Uptake But Not Resorption Alleviates Phosphorus Deficiency Induced By Nitrogen Deposition In Temperate Larix Principis-Rupprechtii Plantations", "description": "Summary
The imbalance between nitrogen (N) and phosphorus (P) deposition may shift temperate ecosystems from N\uffe2\uff80\uff90 to P\uffe2\uff80\uff90limitation. However, it is unclear how the imbalanced N\uffc2\uffa0:\uffc2\uffa0P input affects the strategies of plants to acquire P and, therefore, the growth of plants and the competition among species.
We conducted a 4\uffe2\uff80\uff90yr N\uffe2\uff80\uff90addition experiment in young and mature larch (Larix principis\uffe2\uff80\uff90rupprechtii) stands. Plant growth and P acquisition strategies were assessed for larch and understorey vegetation.
N addition stimulated the aboveground productivity of understorey vegetation in the young stand and larch in the mature stand, with other species unaffected. The competitive advantages of understorey vegetation in the young stand and larch in the mature stand were associated with their high stoichiometric homoeostasis. To maintain the N\uffc2\uffa0:\uffc2\uffa0P homoeostasis of these species, an increase in phosphatase activity but not P resorption efficiency increased the supply of P. Additionally, N addition accelerated P mineralization by decreasing the fungal\uffe2\uff80\uff90to\uffe2\uff80\uff90bacterial ratios and improved uptake of soil P by increasing the arbuscular mycorrhizas\uffe2\uff80\uff90to\uffe2\uff80\uff90ectomycorrhizas ratios.
Our results suggest that plants with high stoichiometric homoeostasis could better cope with N deposition\uffe2\uff80\uff90induced P\uffe2\uff80\uff90deficiency. Although P resorption efficiency showed little plasticity in response, plants activated a variety of P\uffe2\uff80\uff90acquisition pathways to alleviate the P\uffe2\uff80\uff90deficiency caused by N deposition.
", "keywords": ["2. Zero hunger", "Analysis of Variance", "Bacteria", "Nitrogen", "Acid Phosphatase", "Fatty Acids", "Fungi", "Larix", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "Phosphates", "Plant Leaves", "Species Specificity", "Mycorrhizae", "Homeostasis", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Phospholipids"]}, "links": [{"href": "https://doi.org/10.1111/nph.14083"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.14083", "name": "item", "description": "10.1111/nph.14083", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.14083"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-07-11T00:00:00Z"}}, {"id": "10.1111/nph.15119", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:47Z", "type": "Journal Article", "created": "2018-03-31", "title": "Biodiversity of arbuscular mycorrhizal fungi and ecosystem function", "description": "Contents Summary 1059 I. Introduction: pathways of influence and pervasiveness of effects 1060 II. AM fungal richness effects on ecosystem functions 1062 III. Other dimensions of biodiversity 1062 IV. Back to basics \uffe2\uff80\uff93 primary axes of niche differentiation by AM fungi 1066 V. Functional diversity of AM fungi \uffe2\uff80\uff93 a role for biological stoichiometry? 1067 VI. Past, novel and future ecosystems 1068 VII. Opportunities and the way forward 1071 Acknowledgements 1072 References 1072
SummaryArbuscular mycorrhizal (AM) fungi play important functional roles in ecosystems, including the uptake and transfer of nutrients, modification of the physical soil environment and alteration of plant interactions with other biota. Several studies have demonstrated the potential for variation in AM fungal diversity to also affect ecosystem functioning, mainly via effects on primary productivity. Diversity in these studies is usually characterized in terms of the number of species, unique evolutionary lineages or complementary mycorrhizal traits, as well as the ability of plants to discriminate among AM fungi in space and time. However, the emergent outcomes of these relationships are usually indirect, and thus context dependent, and difficult to predict with certainty. Here, we advocate a fungal\uffe2\uff80\uff90centric view of AM fungal biodiversity\uffe2\uff80\uff93ecosystem function relationships that focuses on the direct and specific links between AM fungal fitness and consequences for their roles in ecosystems, especially highlighting functional diversity in hyphal resource economics. We conclude by arguing that an understanding of AM fungal functional diversity is fundamental to determine whether AM fungi have a role in the exploitation of marginal/novel environments (whether past, present or future) and highlight avenues for future research.
", "keywords": ["580", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Biodiversity", "Plants", "15. Life on land", "stoichiometry", "03 medical and health sciences", "Mycorrhizae", "XXXXXX - Unknown", "ecosystems", "global change", "biodiversity"]}, "links": [{"href": "https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.15119"}, {"href": "https://doi.org/10.1111/nph.15119"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.15119", "name": "item", "description": "10.1111/nph.15119", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.15119"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-03-30T00:00:00Z"}}, {"id": "10.1111/nph.17980", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:48Z", "type": "Journal Article", "created": "2022-01-19", "title": "Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi", "description": "Summary
Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore\uffe2\uff80\uff90space, and models of AMF\uffe2\uff80\uff90enhanced P\uffe2\uff80\uff90uptake are poorly validated.
We used synchrotron X\uffe2\uff80\uff90ray computed tomography to visualize mycorrhizas in soil and synchrotron X\uffe2\uff80\uff90ray fluorescence/X\uffe2\uff80\uff90ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling.
We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co\uffe2\uff80\uff90locate with areas of high P and low Al, and preferentially associate with organic\uffe2\uff80\uff90type P species over Al\uffe2\uff80\uff90rich inorganic P.
We discovered that AMF avoid Al\uffe2\uff80\uff90rich areas as a source of P. Sulphur\uffe2\uff80\uff90rich regions were found to be correlated with higher hyphal density and an increased organic\uffe2\uff80\uff90associated P\uffe2\uff80\uff90pool, whilst oxidized S\uffe2\uff80\uff90species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome\uffe2\uff80\uff90related. Our experimentally\uffe2\uff80\uff90validated model led to an estimate of P\uffe2\uff80\uff90uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated \uffe2\uff80\uff93 a result with significant implications for the modelling of plant\uffe2\uff80\uff93soil\uffe2\uff80\uff93AMF interactions.
Biological Market Models are common evolutionary frameworks to understand the maintenance of mutualism in mycorrhizas. \uffe2\uff80\uff98Surplus C\uffe2\uff80\uff99 hypotheses provide an alternative framework where stoichiometry and source\uffe2\uff80\uff93sink dynamics govern mycorrhizal function. A critical difference between these frameworks is whether carbon transfer from plants is regulated by nutrient transfer from fungi or through source\uffe2\uff80\uff93sink dynamics. In this review, we: provide a historical perspective; summarize studies that asked whether plants transfer more carbon to fungi that transfer more nutrients; conduct a meta\uffe2\uff80\uff90analysis to assess whether mycorrhizal plant growth suppressions are related to carbon transfer; and review literature on cellular mechanisms for carbon transfer. In sum, current knowledge does not indicate that carbon transfer from plants is directly regulated by nutrient delivery from fungi. Further, mycorrhizal plant growth responses were linked to nutrient uptake rather than carbon transfer. These findings are more consistent with \uffe2\uff80\uff98Surplus C\uffe2\uff80\uff99 hypotheses than Biological Market Models. However, we also identify research gaps, and future research may uncover a mechanism directly linking carbon and nutrient transfer. Until then, we urge caution when applying economic terminology to describe mycorrhizas. We present a synthesis of ideas, consider knowledge gaps, and suggest experiments to advance the field.Tomato plants can establish symbiotic interactions with arbuscular mycorrhizal fungi (AMF) able to promote plant nutrition and prime systemic plant defenses against pathogens attack; the mechanism involved is known as mycorrhiza-induced resistance (MIR). However, studies on the effect of AMF on viral infection, still limited and not conclusive, indicate that AMF colonization may have a detrimental effect on plant defenses against viruses, so that the term \uffe2\uff80\uff9cmycorrhiza-induced susceptibility\uffe2\uff80\uff9d (MIS) has been proposed for these cases. To expand the case studies to a not yet tested viral family, that is, Bromoviridae, we investigated the effect of the colonization by the AMF Funneliformis mosseae on cucumber mosaic virus (CMV) infection in tomato by phenotypic, physiological, biochemical, and transcriptional analyses. Our results showed that the establishment of a functional AM symbiosis is able to limit symptoms development. Physiological and transcriptomic data highlighted that AMF mitigates the drastic downregulation of photosynthesis-related genes and the reduction of photosynthetic CO2 assimilation rate caused by CMV infection. In parallel, an increase of salicylic acid level and a modulation of reactive oxygen species (ROS)-related genes, toward a limitation of ROS accumulation, was specifically observed in CMV-infected mycorrhizal plants. Overall, our data indicate that the AM symbiosis influences the development of CMV infection in tomato plants and exerts a priming effect able to enhance tolerance to viral infection.", "keywords": ["0301 basic medicine", "plant\u2013virus interaction", "arbuscular mycorrhizal symbiosis", "Microbiology", "Cucumovirus", "Plant Roots", "Article", "03 medical and health sciences", "Solanum lycopersicum", "Gene Expression Regulation", " Plant", "Mycorrhizae", "arbuscular mycorrhizal symbiosis", " cucumber mosaic virus", " Funneliformis mosseae", " gene expression", " priming tolerance", " plant-virus interaction", " RNA sequencing", " Solanum lycopersicum L.", "Photosynthesis", "Symbiosis", "Funneliformis mosseae", "Plant Diseases", "2. Zero hunger", "0303 health sciences", "cucumber mosaic virus", "Fungi", "RNA sequencing", "Carbon Dioxide", "QR1-502", "3. Good health", "Solanum lycopersicum L.", "gene expression", "arbuscular mycorrhizal symbiosis; cucumber mosaic virus; Funneliformis mosseae; gene expression; priming tolerance; plant-virus interaction; RNA sequencing; Solanum lycopersicum L.", "priming tolerance", "Arbuscular mycorrhizal symbiosis; Cucumber mosaic virus; Funneliformis mosseae; Gene expression; Plant-virus interaction; Priming tolerance; RNA sequencing; Solanum lycopersicum L", "Reactive Oxygen Species"]}, "links": [{"href": "http://www.mdpi.com/1999-4915/12/6/675/pdf"}, {"href": "https://iris.cnr.it/bitstream/20.500.14243/410166/1/prod_424799-doc_151509.pdf"}, {"href": "https://iris.unito.it/bitstream/2318/1765477/1/Miozzi%20et%20al%20Viruses%202020.pdf"}, {"href": "https://www.mdpi.com/1999-4915/12/6/675/pdf"}, {"href": "https://doi.org/10.3390/v12060675"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Viruses", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/v12060675", "name": "item", "description": "10.3390/v12060675", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/v12060675"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-06-22T00:00:00Z"}}, {"id": "10.3389/fmicb.2021.634325", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:21:42Z", "type": "Journal Article", "created": "2021-06-17", "title": "How Tillage and Crop Rotation Change the Distribution Pattern of Fungi", "description": "Massive sequencing of fungal communities showed that climatic factors, followed by edaphic and spatial variables, are feasible predictors of fungal richness and community composition. This study, based on a long-term field experiment with tillage and no-tillage management since 1995 and with a crop rotation introduced in 2009, confirmed that tillage practices shape soil properties and impact soil fungal communities. Results highlighted higher biodiversity of saprotrophic fungi in soil sites with low disturbance and an inverse correlation between the biodiversity of ectomycorrhizal and saprotrophic fungi. We speculated how their mutual exclusion could be due to a substrate-mediated niche partitioning or by space segregation. Moreover, where the soil was ploughed, the species were evenly distributed. There was higher spatial variability in the absence of ploughing, with fungal taxa distributed according to a small-scale pattern, corresponding to micro-niches that probably remained undisturbed and heterogeneously distributed. Many differentially represented OTUs in all the conditions investigated were unidentified species or OTUs matching at high taxa level (i.e., phylum, class, order). Among the fungi with key roles in all the investigated conditions, there were several yeast species known to have pronounced endemism in soil and are also largely unidentified. In addition to yeasts, other fungal species emerged as either indicator of a kind of management or as strongly associated with a specific condition. Plant residues played a substantial role in defining the assortment of species.
", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Agriculture", "indicator value", "15. Life on land", "soil yeasts", "rotation", "Microbiology", "QR1-502", "soil", "3. Good health", "FUNGuild database", "03 medical and health sciences", "tillage", "mycorrhizae", "agriculture"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/453431/1/fmicb-12-634325.pdf"}, {"href": "https://doi.org/10.3389/fmicb.2021.634325"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2021.634325", "name": "item", "description": "10.3389/fmicb.2021.634325", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2021.634325"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-17T00:00:00Z"}}, {"id": "10.3389/fmicb.2014.00516", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:21:41Z", "type": "Journal Article", "created": "2014-10-02", "title": "Soil Bacterial Community Composition Altered By Increased Nutrient Availability In Arctic Tundra Soils", "description": "The pool of soil organic carbon (SOC) in the Arctic is disproportionally large compared to those in other biomes. This large quantity of SOC accumulated over millennia due to slow rates of decomposition relative to net primary productivity. Decomposition is constrained by low temperatures and nutrient concentrations, which limit soil microbial activity. We investigated how nutrients limit bacterial and fungal biomass and community composition in organic and mineral soils within moist acidic tussock tundra ecosystems. We sampled two experimental arrays of moist acidic tussock tundra that included fertilized and non-fertilized control plots. One array included plots that had been fertilized annually since 1989 and the other since 2006. Fertilization significantly altered overall bacterial community composition and reduced evenness, to a greater degree in organic than mineral soils, and in the 1989 compared to the 2006 site. The relative abundance of copiotrophic \u03b1-Proteobacteria and \u03b2-Proteobacteria was higher in fertilized than control soils, and oligotrophic Acidobacteria were less abundant in fertilized than control soils at the 1989 site. Fungal community composition was less sensitive to increased nutrient availability, and fungal responses to fertilization were not consistent between soil horizons and sites. We detected two ectomycorrhizal genera, Russula and Cortinarius spp., associated with shrubs. Their relative abundance was not affected by fertilization despite increased dominance of their host plants in the fertilized plots. Our results indicate that fertilization, which has been commonly used to simulate warming in Arctic tundra, has limited applicability for investigating fungal dynamics under warming.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "550", "Bacteria", "Nitrogen", "Fungi", "Phosphorus", "15. Life on land", "Microbiology", "nitrogen", "QR1-502", "soil", "03 medical and health sciences", "fertilization", "13. Climate action", "Fertilization", "Mycorrhizae", "fungi", "Arctic tundra", "phosphorus", "bacteria", "mycorrhizae"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2014.00516"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2014.00516", "name": "item", "description": "10.3389/fmicb.2014.00516", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2014.00516"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-10-02T00:00:00Z"}}, {"id": "10.5061/dryad.8gtht76q3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:22:31Z", "type": "Dataset", "title": "Mycorrhizal effects on decomposition and soil CO2 flux depend on changes in nitrogen availability during forest succession", "description": "Mycorrhizal fungi play a central role in plant nutrition and nutrient cycling, yet our understanding on their effects on free-living microbes, soil carbon (C) decomposition and soil CO2 fluxes remains limited. Here we used trenches lined with mesh screens of varying sizes to isolate mycorrhizal hyphal effects on soil C dynamics in subtropical successional forests. We found that the presence of mycorrhizal hyphae suppressed soil CO2 fluxes by 17% in early-successional forests, but enhanced CO2 losses by 20% and 32% in mid- and late-successional forests, respectively. The inhibitory effects of mycorrhizal fungi on soil CO2 fluxes in the young stands were associated with changes in soil nitrogen (N) mineralization and microbial activities, suggesting that competition between mycorrhizae and saprotrophs for N likely suppressed soil C decomposition. In the mid- and late-successional stands, mycorrhizal enhancement of CO2 release from soil likely resulted from both hyphal respiration and mycorrhizal-induced acceleration of organic matter decay. Synthesis. Our results highlight the sensitivity of mycorrhizal fungi-saprotroph interactions to shifts in nutrient availability and demand, with important consequences for soil carbon dynamics particularly in ecosystems with low nutrient conditions. Incorporating such interactions into models should improve the simulations of forest biogeochemical cycles under global change.", "keywords": ["mycelial respiartion", "soil carbon stabilization", "soil nitrogen", "15. 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