{"type": "FeatureCollection", "features": [{"id": "10.1007/pl00008869", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:15:27Z", "type": "Journal Article", "created": "2006-04-10", "title": "Combined Effects Of Atmospheric Co2 And N Availability On The Belowground Carbon And Nitrogen Dynamics Of Aspen Mesocosms", "description": "It is uncertain whether elevated atmospheric CO2 will increase C storage in terrestrial ecosystems without concomitant increases in plant access to N. Elevated CO2 may alter microbial activities that regulate soil N availability by changing the amount or composition of organic substrates produced by roots. Our objective was to determine the potential for elevated CO2 to change N availability in an experimental plant-soil system by affecting the acquisition of root-derived C by soil microbes. We grew Populus tremuloides (trembling aspen) cuttings for 2 years under two levels of atmospheric CO2 (36.7 and 71.5 Pa) and at two levels of soil N (210 and 970 \u00b5g N g-1). Ambient and twice-ambient CO2 concentrations were applied using open-top chambers, and soil N availability was manipulated by mixing soils differing in organic N content. From June to October of the second growing season, we measured midday rates of soil respiration. In August, we pulse-labeled plants with 14CO2 and measured soil 14CO2 respiration and the 14C contents of plants, soils, and microorganisms after a 6-day chase period. In conjunction with the August radio-labeling and again in October, we used 15N pool dilution techniques to measure in situ rates of gross N mineralization, N immobilization by microbes, and plant N uptake. At both levels of soil N availability, elevated CO2 significantly increased whole-plant and root biomass, and marginally increased whole-plant N capital. Significant increases in soil respiration were closely linked to increases in root biomass under elevated CO2. CO2 enrichment had no significant effect on the allometric distribution of biomass or 14C among plant components, total 14C allocation belowground, or cumulative (6-day) 14CO2 soil respiration. Elevated CO2 significantly increased microbial 14C contents, indicating greater availability of microbial substrates derived from roots. The near doubling of microbial 14C contents at elevated CO2 was a relatively small quantitative change in the belowground C cycle of our experimental system, but represents an ecologically significant effect on the dynamics of microbial growth. Rates of plant N uptake during both 6-day periods in August and October were significantly greater at elevated CO2, and were closely related to fine-root biomass. Gross N mineralization was not affected by elevated CO2. Despite significantly greater rates of N immobilization under elevated CO2, standing pools of microbial N were not affected by elevated CO2, suggesting that N was cycling through microbes more rapidly. Our results contained elements of both positive and negative feedback hypotheses, and may be most relevant to young, aggrading ecosystems, where soil resources are not yet fully exploited by plant roots. If the turnover of microbial N increases, higher rates of N immobilization may not decrease N availability to plants under elevated CO2.", "keywords": ["0106 biological sciences", "root-: biomass-", "Ecology and Evolutionary Biology", "nitrogen-fixation", "Environmental-Sciences)", "01 natural sciences", "nitrogen", "biomass-", "nitrogen-cycle", "nitrogen-", "Microorganisms-", "carbon-14", "124-38-9: CARBON DIOXIDE", "C Cycle", "Spermatophytes-", "Spermatophyta-", "Key Words Atmospheric CO2", "Cellular and Developmental Biology", "Populus Tremuloides Michx", "2. Zero hunger", "carbon-dioxide: atmospheric-", "plant-nutrition", "Climatology- (Environmental-Sciences)", "Angiosperms-", "Angiospermae-", "Plants-", "Natural Resources and Environment", "04 agricultural and veterinary sciences", "global-climate-change", "microbe- (Microorganisms-)", "7727-37-9: NITROGEN", "chemical-composition", "carbon-sequestration", "mineral-uptake", "soil-biology", "Science", "Vascular-Plants", "poplars-", "respiration-", "carbon-dioxide-enrichment", "carbon-dioxide", "Populus-tremuloides [trembling-aspen] (Salicaceae-)", "carbon-cycle", "Health Sciences", "Salicaceae-: Dicotyledones-", "soil-respiration", "content", "Plantae-", "14762-75-5: CARBON-14", "mineralization-", "Molecular", "forest-soils", "15. Life on land", "Rhizodeposition", "soil-flora", "N Cycle", "13. Climate action", "cuttings-", "roots-", "Legacy", "Terrestrial-Ecology (Ecology-", "0401 agriculture", " forestry", " and fisheries", "Dicots-", "ecosystems-"], "contacts": [{"organization": "Mikan, Carl J., Zak, Donald R., Kubiske, Mark E., Pregitzer, Kurt S.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/pl00008869"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/pl00008869", "name": "item", "description": "10.1007/pl00008869", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/pl00008869"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2000-08-23T00:00:00Z"}}, {"id": "10.1007/s10457-016-9914-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:15:57Z", "type": "Journal Article", "created": "2016-02-27", "title": "Effects Of Acacia Seyal And Biochar On Soil Properties And Sorghum Yield In Agroforestry Systems In South Sudan", "description": "We studied the effects of Acacia seyal Del. intercropping and biochar soil amendment on soil physico-chemical properties and sorghum (Sorghum bicolor L.) yields in a two-year field experiment conducted on a silt loam site near Renk in South Sudan. A split-plot design with three replications was used. The main factor was tree-cropping system (dense acacia\u00a0+\u00a0sorghum, scattered acacia\u00a0+\u00a0sorghum, and sole sorghum) and biochar (0 and 10\u00a0Mg\u00a0ha\u22121) was the subplot factor. The two acacia systems had lower soil pH, N and higher C/N ratios compared to the sole sorghum system. Biochar significantly increased soil C, exchangeable K+ contents, field capacity and available water content, but reduced soil exchangeable Ca2+ and effective CEC, and had no effect on soil pH. Acacia intercropping significantly reduced sorghum grain yields while biochar had no significant effect on sorghum yields. The land equivalent ratio (LER) for sorghum yield was 0.3 for both acacia systems in 2011, with or without biochar, but increased in 2012 to 0.6 for the scattered acacia system when combined with biochar. The reduction in sorghum yields by the A. seyal trees was probably due to a combination of competition for water and nutrients and shading. The lack of a yield response to biochar maybe due to insufficient time or too low a dosage. Further research is needed to test for the effects of tree intercropping and biochar and their interactions on soil properties and crop yields in drylands.", "keywords": ["BOREAL LOAMY SAND", "2. Zero hunger", "AGRONOMIC PERFORMANCE", "Land equivalent ratio (LER)", "Forestry", "04 agricultural and veterinary sciences", "15. Life on land", "Agronomy", "NORTH KORDOFAN STATE", "Biochar", "BLUE-NILE REGION", "ORGANIC-MATTER", "CROP YIELDS", "BIOLOGICAL NITROGEN-FIXATION", "Savanna", "Acacia seyal", "TREES", "0401 agriculture", " forestry", " and fisheries", "NERE PARKIA-BIGLOBOSA", "Tree intercropping", "BURKINA-FASO"]}, "links": [{"href": "https://doi.org/10.1007/s10457-016-9914-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agroforestry%20Systems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10457-016-9914-2", "name": "item", "description": "10.1007/s10457-016-9914-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10457-016-9914-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-27T00:00:00Z"}}, {"id": "10.1007/s10705-008-9174-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:16:06Z", "type": "Journal Article", "created": "2008-03-21", "title": "A Comparison Between Legume Technologies And Fallow, And Their Effects On Maize And Soil Traits, In Two Distinct Environments Of The West African Savannah", "description": "Legume\u2013maize rotation and maize nitrogen (N)-response trials were carried out simultaneously from 1998 to 2004 in two distinct agro-ecological environments of West Africa: the humid derived savannah (Ibadan) and the drier northern Guinea savannah (Zaria). In the N-response trial, maize was grown annually receiving urea N at 0, 30, 60, 90 and 120 kg N ha\u22121. In Ibadan, maize production increased with N fertilization, but mean annual grain yield declined over the course of the trial. In Zaria, no response to N treatments was observed initially, and an increase in the phosphorus (P) and sulphur (S) fertilizer application rate was required to increase yield across treatments and obtain a response to N applications, stressing the importance of non-N fertilizers in the savannah. In the rotation trial, a 2-year natural fallow\u2013maize rotation was compared with maize rotated with different legume types: green manure, forage, dual-purpose, and grain legumes. The cultivation of some legume types resulted in a greater annual maize production relative to the fallow\u2013maize combination and corresponding treatments in the N-response trial, while there was no gain in maize yield with other legume types. Large differences in the residual effects from legumes and fallow were also observed between sites, indicting a need for site-specific land management recommendations. In Ibadan, cultivation of maize after the forage legume (Stylosanthes guianensis) achieved the highest yield. The natural fallow\u2013maize rotation had improved soil characteristics (Bray-I P, exchangeable potassium, calcium and magnesium) at the end of the trial relative to legume\u2013maize rotations, and natural fallow resulted in higher maize yields than the green manure legume (Pueraria phaseoloides). In Zaria, maize following dual-purpose soybean achieved the highest mean yield. At both sites, variation in aboveground N and P dynamics of the legume and fallow vegetation could only partly explain the different residual effects on maize.", "keywords": ["2. Zero hunger", "forage legumes", "agropastoral systems", "northern guinea savanna", "livestock systems", "Soil Science", "biological nitrogen-fixation", "increased crop production", "continuous cultivation", "04 agricultural and veterinary sciences", "15. Life on land", "0401 agriculture", " forestry", " and fisheries", "farming systems", "fertility management", "organic-matter", "Agronomy and Crop Science"], "contacts": [{"organization": "Franke, A.C., Laberge, G., Oyewole, B.D., Schulz, S., Tobe, O.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s10705-008-9174-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nutrient%20Cycling%20in%20Agroecosystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10705-008-9174-2", "name": "item", "description": "10.1007/s10705-008-9174-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10705-008-9174-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-03-22T00:00:00Z"}}, {"id": "10.1007/s11104-022-05508-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:16:26Z", "type": "Journal Article", "created": "2022-06-22", "title": "Harnessing belowground processes for sustainable intensification of agricultural systems", "description": "Abstract <p>Increasing food demand coupled with climate change pose a great challenge to agricultural systems. In this review we summarize recent advances in our knowledge of how plants, together with their associated microbiota, shape rhizosphere processes. We address (molecular) mechanisms operating at the plant\uffe2\uff80\uff93microbe-soil interface and aim to link this knowledge with actual and potential avenues for intensifying agricultural systems, while at the same time reducing irrigation water, fertilizer inputs and pesticide use. Combining in-depth knowledge about above and belowground plant traits will not only significantly advance our mechanistic understanding of involved processes but also allow for more informed decisions regarding agricultural practices and plant breeding. Including belowground plant-soil-microbe interactions in our breeding efforts will help to select crops resilient to abiotic and biotic environmental stresses and ultimately enable us to produce sufficient food in a more sustainable agriculture in the upcoming decades.</p", "keywords": ["0301 basic medicine", "BIOLOGICAL NITRIFICATION INHIBITION", "PHOSPHATE SOLUBILIZING BACTERIA", "Plant-plant interaction", "Rhizobiome", "MEDIATED PH CHANGES", "Review Article", "Plant health", "MEMBRANE H+-ATPASE", "12. Responsible consumption", "03 medical and health sciences", "Soil health", "Soil structure", "C sequestration", "SDG 13 - Climate Action", "SDG 2 \u2013 Kein Hunger", "106026 Ecosystem research", "Plant nutrition", "SDG 2 - Zero Hunger", "2. Zero hunger", "0303 health sciences", "Water availability", "Root exudation", "JASMONIC ACID", "15. Life on land", "Microbes", "Intercropping", "106026 \u00d6kosystemforschung", "PLANT-GROWTH", "13. Climate action", "ROOT BORDER CELLS", "SDG 13 \u2013 Ma\u00dfnahmen zum Klimaschutz", "C cycling", "INNATE IMMUNITY", "NITROGEN-FIXATION", "ORGANIC-ACIDS"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s11104-022-05508-z.pdf"}, {"href": "https://doi.org/10.1007/s11104-022-05508-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-022-05508-z", "name": "item", "description": "10.1007/s11104-022-05508-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-022-05508-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-22T00:00:00Z"}}, {"id": "10.1016/j.fcr.2012.07.014", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-25T16:17:54Z", "type": "Journal Article", "created": "2012-08-09", "title": "Maize\u2013Grain Legume Intercropping Is An Attractive Option For Ecological Intensification That Reduces Climatic Risk For Smallholder Farmers In Central Mozambique", "description": "Abstract   Many farmers in central Mozambique intercrop maize with grain legumes as a means to improve food security and income. The objective of this study was to understand the farming system, and to evaluate the suitability of maize\u2013legume intercropping to alleviate the biophysical and socio-economic constraints faced by smallholder farmers in Ruaca and Vunduzi villages, central Mozambique. To achieve this we characterised the farming systems and measured grain yields, rainfall infiltration, economic returns and acceptability of maize\u2013legume intercrops under different N and P application rates. Two intercropping strategies were tested: (a) an additive design of within-row intercropping in which legume was intercropped with alternating hills of maize within the same row; maize plant population was the same as sole crop maize, and (b) a substitutive design with distinct alternating rows of maize and legume (local practice). Fertiliser treatments imposed on all treatments were: (i) no fertiliser, (ii) 20\u00a0kg\u00a0P\u00a0ha \u22121 , (iii) 20\u00a0kg\u00a0P\u00a0ha \u22121 \u00a0+\u00a030\u00a0kg\u00a0N\u00a0ha \u22121 , and (iv) 20\u00a0kg\u00a0P\u00a0ha \u22121 \u00a0+\u00a060\u00a0kg\u00a0N\u00a0ha \u22121 . Intercrops were relatively more productive than the corresponding sole crops; land equivalent ratios (LER) for within-row intercropping ranged between 1.1 and 2.4, and between 1.0 and 1.9 for distinct-row intercropping. Average maize yield penalty for intercropping maize and pigeonpea in the within-row was small (8%) compared with 50% in the distinct-row design; average (season\u00a0\u00d7\u00a0fertiliser) sole maize yield was 3.2\u00a0t\u00a0ha \u22121 . Intercropping maize and cowpea in within-row led to maize yield loss of only 6%, whereas distinct-row intercropping reduced maize yield by 25% from 2.1\u00a0t\u00a0ha \u22121  of sole maize (season\u00a0\u00d7\u00a0fertiliser). Cowpea yield was less affected by intercropping: sole cowpea had an average yield of 0.9\u00a0t\u00a0ha \u22121 , distinct-row intercropping (0.8\u00a0t\u00a0ha \u22121 ) and the within-row intercropping yielded 0.9\u00a0t\u00a0ha \u22121 . Legumes were comparatively less affected by the long dry spells which were prevalent during the study period. Response to N and P fertiliser was weak due to poor rainfall distribution. In the third season, maize in rotation with pigeonpea and without N fertiliser application yielded 5.6\u00a0t\u00a0ha \u22121 , eight times more than continuous maize which was severely infested by striga ( Striga asiatica ) and yielded only 0.7\u00a0t\u00a0ha \u22121 . Rainfall infiltration increased from 6\u00a0mm\u00a0h \u22121  to 22\u00a0mm\u00a0h \u22121  with long-term maize\u2013legume intercropping due to a combination of good quality biomass production which provided mulch combined with no tillage. Intercropping maize and pigeonpea was profitable with a rate of return of at least 343% over sole maize cropping. Farmers preferred the within-row maize\u2013legume intercropping with an acceptability score of 84% because of good yields for both maize and legume. Intercropping increased the labour required for weeding by 36% compared with the sole crops. Farmers in Ruaca faced labour constraints due to extensification thus maize\u2013pigeonpea intercropping may improve productivity and help reduce the area cultivated. In Vunduzi, land limitation was a major problem and intensification through legumes is amongst the few feasible options to increase both production and productivity. The late maturity of pigeonpea means that free-grazing of cattle has to be delayed, which allows farmers to retain crop residues in the fields as mulch if they choose to; this allows the use of no-tillage practises. We conclude that maize\u2013legume intercropping has potential to: (a) reduce the risk of crop failure, (b) improve productivity and income, and (c) increase food security in vulnerable production systems, and is a feasible entry point to ecological intensification.", "keywords": ["0106 biological sciences", "F08 - Syst\u00e8mes et modes de culture", "rendement des cultures", "agro\u00e9cologie", "petite exploitation agricole", "extensification", "nitrogen-fixation", "01 natural sciences", "7. Clean energy", "630", "analyse \u00e9conomique", "l\u00e9gume sec", "http://aims.fao.org/aos/agrovoc/c_6214", "striga", "syst\u00e8me de culture", "intensification", "2. Zero hunger", "http://aims.fao.org/aos/agrovoc/c_10967", "soil fertility", "1. No poverty", "facteur climatique", "04 agricultural and veterinary sciences", "Sorghum bicolor", "resource capture", "http://aims.fao.org/aos/agrovoc/c_92381", "rentabilit\u00e9", "conservation agriculture", "http://aims.fao.org/aos/agrovoc/c_8504", "s\u00e9curit\u00e9 alimentaire", "http://aims.fao.org/aos/agrovoc/c_13199", "E16 - \u00c9conomie de la production", "Vigna unguiculata", "crop-rotation", "http://aims.fao.org/aos/agrovoc/c_29554", "Cenchrus americanus", "Zea mays", "http://aims.fao.org/aos/agrovoc/c_7247", "http://aims.fao.org/aos/agrovoc/c_10176", "Life Science", "decomposition", "15. Life on land", "yield", "Maize", "cowpea", "http://aims.fao.org/aos/agrovoc/c_3351", "http://aims.fao.org/aos/agrovoc/c_33484", "http://aims.fao.org/aos/agrovoc/c_33485", "systems", "http://aims.fao.org/aos/agrovoc/c_3910", "0401 agriculture", " forestry", " and fisheries", "http://aims.fao.org/aos/agrovoc/c_2469", "culture intercalaire", "http://aims.fao.org/aos/agrovoc/c_1971", "http://aims.fao.org/aos/agrovoc/c_4964", "http://aims.fao.org/aos/agrovoc/c_8247", "http://aims.fao.org/aos/agrovoc/c_7113"]}, "links": [{"href": "https://doi.org/10.1016/j.fcr.2012.07.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Field%20Crops%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.fcr.2012.07.014", "name": "item", "description": "10.1016/j.fcr.2012.07.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.fcr.2012.07.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-09-01T00:00:00Z"}}, {"id": "10.1111/1462-2920.13954", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-25T16:21:35Z", "type": "Journal Article", "created": "2022-10-18", "title": "Application of stable-isotope labelling techniques for the detection of active diazotrophs", "description": "Summary<p>Investigating active participants in the fixation of dinitrogen gas is vital as N is often a limiting factor for primary production. Biological nitrogen fixation is performed by a diverse guild of bacteria and archaea (diazotrophs), which can be free\uffe2\uff80\uff90living or symbionts. Free\uffe2\uff80\uff90living diazotrophs are widely distributed in the environment, yet our knowledge about their identity and ecophysiology is still limited. A major challenge in investigating this guild is inferring activity from genetic data as this process is highly regulated. To address this challenge, we evaluated and improved several 15N\uffe2\uff80\uff90based methods for detecting N2 fixation activity (with a focus on soil samples) and studying active diazotrophs. We compared the acetylene reduction assay and the 15N2 tracer method and demonstrated that the latter is more sensitive in samples with low activity. Additionally, tracing 15N into microbial RNA provides much higher sensitivity compared to bulk soil analysis. Active soil diazotrophs were identified with a 15N\uffe2\uff80\uff90RNA\uffe2\uff80\uff90SIP approach optimized for environmental samples and benchmarked to 15N\uffe2\uff80\uff90DNA\uffe2\uff80\uff90SIP. Lastly, we investigated the feasibility of using SIP\uffe2\uff80\uff90Raman microspectroscopy for detecting 15N\uffe2\uff80\uff90labelled cells. Taken together, these tools allow identifying and investigating active free\uffe2\uff80\uff90living diazotrophs in a highly sensitive manner in diverse environments, from bulk to the single\uffe2\uff80\uff90cell level.</p>", "keywords": ["Spectrum Analysis", " Raman", "BIOLOGICAL SOIL CRUSTS", "106005 Bioinformatik", "106023 Molekularbiologie", "Nitrogen Fixation", "REVEALS", "FLUORESCENCE", "Research Articles", "Soil Microbiology", "106022 Mikrobiologie", "SPECTROSCOPY", "Bacteria", "Nitrogen Isotopes", "106003 Biodiversity research", "106023 Molecular biology", "GENETIC-REGULATION", "Archaea", "6. Clean water", "SURFACE-ENHANCED RAMAN", "COMMUNITY", "106003 Biodiversit\u00e4tsforschung", "13. Climate action", "Isotope Labeling", "106022 Microbiology", "NITROGEN-FIXATION", "106005 Bioinformatics", "RIBOSOMAL-RNA", "N-2 FIXATION"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13954"}, {"href": "https://doi.org/10.1111/1462-2920.13954"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.13954", "name": "item", "description": "10.1111/1462-2920.13954", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.13954"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-15T00:00:00Z"}}, {"id": "10.1023/b:plso.0000037053.05902.60", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-06-25T16:19:59Z", "type": "Journal Article", "created": "2004-08-06", "title": "Woody Legume Fallow Productivity, Biological N-2-Fixation And Residual Benefits To Two Successive Maize Crops In Zimbabwe", "description": "Three woody legumes were planted as two-year 'improved fallows' to evaluate their residual nitrogen (N) effects on two subsequent maize crops under minimum and conventional tillage management. Maize monoculture and cowpea-maize-maize sequence treatments were included as controls. N-2-fixation was estimated using the N-15 natural abundance method to allow the N contribution from the fallows to be partitioned into N2-fixation and soil N capture. Acacia angustissima accumulated the largest amount of both below-ground and above-ground biomass during the 2-year growth period. Using Hyparrhenia grass as the reference plant, the proportion of N-2-fixed in litter was 56, 55, 84 and 58% for Acacia, Sesbania sesban, Cajanus cajan, and cowpea, respectively, resulting in inputs of biologically fixed N of 122, 84, 96 and 28 kg N ha(-1). Maize growth following the legumes for two subsequent cropping seasons was in most cases not directly related to the N inputs. The first year maize crop was severely infested with cutworms (Agrotis sp.) in the Sesbania and Acacia plots, while the second season maize was affected by drought particularly in the Acacia plots where there was high moisture demand by the re-growing trees. During the second season, N uptake by maize following Sesbania was significantly higher than the rest of the treatments. Conventional tillage resulted in better yields than minimum tillage across all treatments and in both seasons. It can be concluded that (1) improved legume fallow system results in net positive soil N balances, (2) despite large inputs of litter and continued recycling of N during the cropping phase, legume species that re-grow after cutting can result in depressed yields in drought seasons, and (3) tillage is beneficial even after improved legume fallows.", "keywords": ["2. Zero hunger", "0106 biological sciences", "shrub", "n-15 natural-abundance", "root development", "soil fertility", "0401 agriculture", " forestry", " and fisheries", "nitrogen-fixation", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "cassia-siamea", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1023/b:plso.0000037053.05902.60"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1023/b:plso.0000037053.05902.60", "name": "item", "description": "10.1023/b:plso.0000037053.05902.60", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1023/b:plso.0000037053.05902.60"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-05-01T00:00:00Z"}}, {"id": "10.1073/pnas.0509038103", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:20:46Z", "type": "Journal Article", "created": "2006-04-14", "title": "Element interactions limit soil carbon storage", "description": "<p>             Rising levels of atmospheric CO             2             are thought to increase C sinks in terrestrial ecosystems. The potential of these sinks to mitigate CO             2             emissions, however, may be constrained by nutrients. By using metaanalysis, we found that elevated CO             2             only causes accumulation of soil C when N is added at rates well above typical atmospheric N inputs. Similarly, elevated CO             2             only enhances N             2             fixation, the major natural process providing soil N input, when other nutrients (e.g., phosphorus, molybdenum, and potassium) are added. Hence, soil C sequestration under elevated CO             2             is constrained both directly by N availability and indirectly by nutrients needed to support N             2             fixation.           </p>", "keywords": ["Greenhouse Effect", "Nitrogen", "cycles", "fine roots", "Plant Development", "01 natural sciences", "forest", "Soil", "Nitrogen Fixation", "elevated atmospheric co2", "Ecosystem", "0105 earth and related environmental sciences", "model", "biological nitrogen-fixation", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Plants", "15. Life on land", "Carbon", "6. Clean water", "13. Climate action", "climate-change", "0401 agriculture", " forestry", " and fisheries", "grassland", "ecosystem responses", "metaanalysis"]}, "links": [{"href": "https://doi.org/10.1073/pnas.0509038103"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.0509038103", "name": "item", "description": "10.1073/pnas.0509038103", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.0509038103"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-04-25T00:00:00Z"}}, {"id": "10.1088/1748-9326/aaeae7", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:21:08Z", "type": "Journal Article", "created": "2018-10-24", "title": "Using research networks to create the comprehensive datasets needed to assess nutrient availability as a key determinant of terrestrial carbon cycling", "description": "Open AccessA wide range of research shows that nutrient availability strongly influences terrestrial carbon (C) cycling and shapes ecosystem responses to environmental changes and hence terrestrial feedbacks to climate. Nonetheless, our understanding of nutrient controls remains far from complete and poorly quantified, at least partly due to a lack of informative, comparable, and accessible datasets at regional-to-global scales. A growing research infrastructure of multi-site networks are providing valuable data on C fluxes and stocks and are monitoring their responses to global environmental change and measuring responses to experimental treatments. These networks thus provide an opportunity for improving our understanding of C-nutrient cycle interactions and our ability to model them. However, coherent information on how nutrient cycling interacts with observed C cycle patterns is still generally lacking. Here, we argue that complementing available C-cycle measurements from monitoring and experimental sites with data characterizing nutrient availability will greatly enhance their power and will improve our capacity to forecast future trajectories of terrestrial C cycling and climate. Therefore, we propose a set of complementary measurements that are relatively easy to conduct routinely at any site or experiment and that, in combination with C cycle observations, can provide a robust characterization of the effects of nutrient availability across sites. In addition, we discuss the power of different observable variables for informing the formulation of models and constraining their predictions. Most widely available measurements of nutrient availability often do not align well with current modelling needs. This highlights the importance to foster the interaction between the empirical and modelling communities for setting future research priorities.", "keywords": ["Global vegetation models", "550", "manipulation experiments", "Terrestrial-Aquatic Linkages", "Kolefni", "01 natural sciences", "Nutrient cycle", "Agricultural and Biological Sciences", "Terrestrial ecosystem", "SDG 13 - Climate Action", "Climate change", "Jar\u00f0vegur", "Environmental resource management", "Global change", "General Environmental Science", "SDG 15 - Life on Land", "Carbon-nutrient cycle interactions", "2. Zero hunger", "Data syntheses", "Global and Planetary Change", "Ecology", "Geography", "Physics", "Life Sciences", "Application of Stable Isotopes in Trophic Ecology", "Cycling", "Carbon cycle", "04 agricultural and veterinary sciences", "Chemistry", "ORGANIC-MATTER", "Archaeology", "Physical Sciences", "Nutrient availability", "NET PRIMARY PRODUCTIVITY", "Ecosystem Functioning", "570", "LAND", "TROPICAL RAIN-FOREST", "carbon-nutrient cycle interactions", "data syntheses", "Soil Science", "Environmental science", "[SDU] Sciences of the Universe [physics]", "SOIL-PHOSPHORUS AVAILABILITY", "global vegetation models", "SDG 3 - Good Health and Well-being", "nutrients", "USE EFFICIENCY", "SDG 7 - Affordable and Clean Energy", "GLOBAL CHANGE", "Key (lock)", "Biology", "Ecosystem", "Manipulation experiments", "0105 earth and related environmental sciences", "Renewable Energy", " Sustainability and the Environment", "Ecosystem Structure", "Public Health", " Environmental and Occupational Health", "Nutrients", "15. Life on land", "Computer science", "[SDU]Sciences of the Universe [physics]", "13. Climate action", "ECOSYSTEM RESPONSES", "FOS: Biological sciences", "Global Methane Emissions and Impacts", "Environmental Science", "0401 agriculture", " forestry", " and fisheries", "NITROGEN-FIXATION", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "Nutrient Limitation", "ELEVATED CO2", "Nutrient"]}, "links": [{"href": "https://doi.org/10.1088/1748-9326/aaeae7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1088/1748-9326/aaeae7", "name": "item", "description": "10.1088/1748-9326/aaeae7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1088/1748-9326/aaeae7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-07T00:00:00Z"}}, {"id": "10.1093/jxb/erq249", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:21:14Z", "type": "Journal Article", "created": "2010-08-27", "title": "Plant Physiology And Proteomics Reveals The Leaf Response To Drought In Alfalfa (Medicago Sativa L.)", "description": "Despite its relevance, protein regulation, metabolic adjustment, and the physiological status of plants under drought is not well understood in relation to the role of nitrogen fixation in nodules. In this study, nodulated alfalfa plants were exposed to drought conditions. The study determined the physiological, metabolic, and proteomic processes involved in photosynthetic inhibition in relation to the decrease in nitrogenase (N(ase)) activity. The deleterious effect of drought on alfalfa performance was targeted towards photosynthesis and N(ase) activity. At the leaf level, photosynthetic inhibition was mainly caused by the inhibition of Rubisco. The proteomic profile and physiological measurements revealed that the reduced carboxylation capacity of droughted plants was related to limitations in Rubisco protein content, activation state, and RuBP regeneration. Drought also decreased amino acid content such as asparagine, and glutamic acid, and Rubisco protein content indicating that N availability limitations were caused by N(ase) activity inhibition. In this context, drought induced the decrease in Rubisco binding protein content at the leaf level and proteases were up-regulated so as to degrade Rubisco protein. This degradation enabled the reallocation of the Rubisco-derived N to the synthesis of amino acids with osmoregulant capacity. Rubisco degradation under drought conditions was induced so as to remobilize Rubisco-derived N to compensate for the decrease in N associated with N(ase) inhibition. Metabolic analyses showed that droughted plants increased amino acid (proline, a major compound involved in osmotic regulation) and soluble sugar (D-pinitol) levels to contribute towards the decrease in osmotic potential (\u03a8(s)). At the nodule level, drought had an inhibitory effect on N(ase) activity. This decrease in N(ase) activity was not induced by substrate shortage, as reflected by an increase in total soluble sugars (TSS) in the nodules. Proline accumulation in the nodule could also be associated with an osmoregulatory response to drought and might function as a protective agent against ROS. In droughted nodules, the decrease in N(2) fixation was caused by an increase in oxygen resistance that was induced in the nodule. This was a mechanism to avoid oxidative damage associated with reduced respiration activity and the consequent increase in oxygen content. This study highlighted that even though drought had a direct effect on leaves, the deleterious effects of drought on nodules also conditioned leaf responsiveness.", "keywords": ["Proteomics", "0301 basic medicine", "570", "Rubisco", "Proteome", "[SDV]Life Sciences [q-bio]", "proteome", "N-2 FIXATION RESPONSE", "drought", "N2 fixation", "03 medical and health sciences", "XANTHOPHYLL CYCLE", "N-2 fixation", "2-CYSTEINE PEROXIREDOXIN", "Nitrogenase", "oxidative stress", "AMINO-ACIDS", "Photosynthesis", "climate", "agriculture", "Plant Proteins", "580", "N remobilization", "2. Zero hunger", "0303 health sciences", "photosynthesis", "PINITOL ACCUMULATION", "Drought", "RIBULOSE-1", "5-BISPHOSPHATE CARBOXYLASE-OXYGENASE", "Water", "BRASSICA-NAPUS", "N(O)-TERT-BUTYLDIMETHYLSILYL DERIVATIVES", "15. Life on land", "Research Papers", "6. Clean water", "Droughts", "[SDV] Life Sciences [q-bio]", "Plant Leaves", "nitrogen fixation", "Oxidative stress", "rubisco", "NITROGEN-FIXATION", "WATER-WATER CYCLE", "Medicago sativa"]}, "links": [{"href": "https://doi.org/10.1093/jxb/erq249"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jxb/erq249", "name": "item", "description": "10.1093/jxb/erq249", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jxb/erq249"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-25T00:00:00Z"}}, {"id": "10.1111/1462-2920.16213", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:21:35Z", "type": "Journal Article", "created": "2022-10-18", "title": "Application of stable\u2010isotope labelling techniques for the detection of active diazotrophs", "description": "Summary<p>Investigating active participants in the fixation of dinitrogen gas is vital as N is often a limiting factor for primary production. Biological nitrogen fixation is performed by a diverse guild of bacteria and archaea (diazotrophs), which can be free\uffe2\uff80\uff90living or symbionts. Free\uffe2\uff80\uff90living diazotrophs are widely distributed in the environment, yet our knowledge about their identity and ecophysiology is still limited. A major challenge in investigating this guild is inferring activity from genetic data as this process is highly regulated. To address this challenge, we evaluated and improved several 15N\uffe2\uff80\uff90based methods for detecting N2 fixation activity (with a focus on soil samples) and studying active diazotrophs. We compared the acetylene reduction assay and the 15N2 tracer method and demonstrated that the latter is more sensitive in samples with low activity. Additionally, tracing 15N into microbial RNA provides much higher sensitivity compared to bulk soil analysis. Active soil diazotrophs were identified with a 15N\uffe2\uff80\uff90RNA\uffe2\uff80\uff90SIP approach optimized for environmental samples and benchmarked to 15N\uffe2\uff80\uff90DNA\uffe2\uff80\uff90SIP. Lastly, we investigated the feasibility of using SIP\uffe2\uff80\uff90Raman microspectroscopy for detecting 15N\uffe2\uff80\uff90labelled cells. Taken together, these tools allow identifying and investigating active free\uffe2\uff80\uff90living diazotrophs in a highly sensitive manner in diverse environments, from bulk to the single\uffe2\uff80\uff90cell level.</p", "keywords": ["Spectrum Analysis", " Raman", "BIOLOGICAL SOIL CRUSTS", "106005 Bioinformatik", "106023 Molekularbiologie", "Nitrogen Fixation", "REVEALS", "FLUORESCENCE", "Research Articles", "Soil Microbiology", "106022 Mikrobiologie", "SPECTROSCOPY", "Bacteria", "Nitrogen Isotopes", "106003 Biodiversity research", "106023 Molecular biology", "GENETIC-REGULATION", "Archaea", "6. Clean water", "SURFACE-ENHANCED RAMAN", "COMMUNITY", "106003 Biodiversit\u00e4tsforschung", "13. Climate action", "Isotope Labeling", "106022 Microbiology", "NITROGEN-FIXATION", "106005 Bioinformatics", "RIBOSOMAL-RNA", "N-2 FIXATION"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13954"}, {"href": "https://doi.org/10.1111/1462-2920.16213"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.16213", "name": "item", "description": "10.1111/1462-2920.16213", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.16213"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-15T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2006.01240.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:21:56Z", "type": "Journal Article", "created": "2006-09-26", "title": "Interactions between plant growth and soil nutrient cycling under elevated CO2: a meta-analysis", "description": "Abstract<p>free air carbon dioxide enrichment (FACE) and open top chamber (OTC) studies are valuable tools for evaluating the impact of elevated atmospheric CO2 on nutrient cycling in terrestrial ecosystems. Using meta\uffe2\uff80\uff90analytic techniques, we summarized the results of 117 studies on plant biomass production, soil organic matter dynamics and biological N2 fixation in FACE and OTC experiments. The objective of the analysis was to determine whether elevated CO2 alters nutrient cycling between plants and soil and if so, what the implications are for soil carbon (C) sequestration. Elevated CO2 stimulated gross N immobilization by 22%, whereas gross and net N mineralization rates remained unaffected. In addition, the soil C\uffe2\uff80\uff83:\uffe2\uff80\uff83N ratio and microbial N contents increased under elevated CO2 by 3.8% and 5.8%, respectively. Microbial C contents and soil respiration increased by 7.1% and 17.7%, respectively. Despite the stimulation of microbial activity, soil C input still caused soil C contents to increase by 1.2%\uffe2\uff80\uff83yr\uffe2\uff88\uff921. Namely, elevated CO2 stimulated overall above\uffe2\uff80\uff90 and belowground plant biomass by 21.5% and 28.3%, respectively, thereby outweighing the increase in CO2 respiration. In addition, when comparing experiments under both low and high N availability, soil C contents (+2.2%\uffe2\uff80\uff83yr\uffe2\uff88\uff921) and above\uffe2\uff80\uff90 and belowground plant growth (+20.1% and+33.7%) only increased under elevated CO2 in experiments receiving the high N treatments. Under low N availability, above\uffe2\uff80\uff90 and belowground plant growth increased by only 8.8% and 14.6%, and soil C contents did not increase. Nitrogen fixation was stimulated by elevated CO2 only when additional nutrients were supplied. These results suggest that the main driver of soil C sequestration is soil C input through plant growth, which is strongly controlled by nutrient availability. In unfertilized ecosystems, microbial N immobilization enhances acclimation of plant growth to elevated CO2 in the long\uffe2\uff80\uff90term. Therefore, increased soil C input and soil C sequestration under elevated CO2 can only be sustained in the long\uffe2\uff80\uff90term when additional nutrients are supplied.</p>", "keywords": ["2. Zero hunger", "enrichment", "microbial biomass", "atmospheric carbon-dioxide", "nitrogen-fixation", "dynamics", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "forest", "tallgrass prairie", "13. Climate action", "responses", "0401 agriculture", " forestry", " and fisheries", "organic-matter", "respiration"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2006.01240.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2006.01240.x", "name": "item", "description": "10.1111/j.1365-2486.2006.01240.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2006.01240.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-09-26T00:00:00Z"}}, {"id": "20.500.11815/1261", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:32:22Z", "type": "Journal Article", "created": "2018-10-24", "title": "Using research networks to create the comprehensive datasets needed to assess nutrient availability as a key determinant of terrestrial carbon cycling", "description": "Open AccessA wide range of research shows that nutrient availability strongly influences terrestrial carbon (C) cycling and shapes ecosystem responses to environmental changes and hence terrestrial feedbacks to climate. Nonetheless, our understanding of nutrient controls remains far from complete and poorly quantified, at least partly due to a lack of informative, comparable, and accessible datasets at regional-to-global scales. A growing research infrastructure of multi-site networks are providing valuable data on C fluxes and stocks and are monitoring their responses to global environmental change and measuring responses to experimental treatments. These networks thus provide an opportunity for improving our understanding of C-nutrient cycle interactions and our ability to model them. However, coherent information on how nutrient cycling interacts with observed C cycle patterns is still generally lacking. Here, we argue that complementing available C-cycle measurements from monitoring and experimental sites with data characterizing nutrient availability will greatly enhance their power and will improve our capacity to forecast future trajectories of terrestrial C cycling and climate. Therefore, we propose a set of complementary measurements that are relatively easy to conduct routinely at any site or experiment and that, in combination with C cycle observations, can provide a robust characterization of the effects of nutrient availability across sites. In addition, we discuss the power of different observable variables for informing the formulation of models and constraining their predictions. Most widely available measurements of nutrient availability often do not align well with current modelling needs. This highlights the importance to foster the interaction between the empirical and modelling communities for setting future research priorities.", "keywords": ["Global vegetation models", "550", "manipulation experiments", "Terrestrial-Aquatic Linkages", "Kolefni", "01 natural sciences", "Nutrient cycle", "Agricultural and Biological Sciences", "Terrestrial ecosystem", "SDG 13 - Climate Action", "Climate change", "Jar\u00f0vegur", "Environmental resource management", "Global change", "General Environmental Science", "SDG 15 - Life on Land", "Carbon-nutrient cycle interactions", "2. Zero hunger", "Data syntheses", "Global and Planetary Change", "Ecology", "Geography", "Physics", "Life Sciences", "Application of Stable Isotopes in Trophic Ecology", "Cycling", "Carbon cycle", "04 agricultural and veterinary sciences", "Chemistry", "ORGANIC-MATTER", "Archaeology", "Physical Sciences", "Nutrient availability", "NET PRIMARY PRODUCTIVITY", "Ecosystem Functioning", "570", "LAND", "TROPICAL RAIN-FOREST", "carbon-nutrient cycle interactions", "data syntheses", "Soil Science", "Environmental science", "[SDU] Sciences of the Universe [physics]", "SOIL-PHOSPHORUS AVAILABILITY", "global vegetation models", "SDG 3 - Good Health and Well-being", "nutrients", "USE EFFICIENCY", "SDG 7 - Affordable and Clean Energy", "GLOBAL CHANGE", "Key (lock)", "Biology", "Ecosystem", "Manipulation experiments", "0105 earth and related environmental sciences", "Renewable Energy", " Sustainability and the Environment", "Ecosystem Structure", "Public Health", " Environmental and Occupational Health", "Nutrients", "15. Life on land", "Computer science", "[SDU]Sciences of the Universe [physics]", "13. Climate action", "ECOSYSTEM RESPONSES", "FOS: Biological sciences", "Global Methane Emissions and Impacts", "Environmental Science", "0401 agriculture", " forestry", " and fisheries", "NITROGEN-FIXATION", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "Nutrient Limitation", "ELEVATED CO2", "Nutrient"]}, "links": [{"href": "https://doi.org/20.500.11815/1261"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.11815/1261", "name": "item", "description": "20.500.11815/1261", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.11815/1261"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-07T00:00:00Z"}}, {"id": "2764305888", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-25T16:33:03Z", "type": "Journal Article", "created": "2022-10-18", "title": "Application of stable\u2010isotope labelling techniques for the detection of active diazotrophs", "description": "Summary<p>Investigating active participants in the fixation of dinitrogen gas is vital as N is often a limiting factor for primary production. Biological nitrogen fixation is performed by a diverse guild of bacteria and archaea (diazotrophs), which can be free\uffe2\uff80\uff90living or symbionts. Free\uffe2\uff80\uff90living diazotrophs are widely distributed in the environment, yet our knowledge about their identity and ecophysiology is still limited. A major challenge in investigating this guild is inferring activity from genetic data as this process is highly regulated. To address this challenge, we evaluated and improved several 15N\uffe2\uff80\uff90based methods for detecting N2 fixation activity (with a focus on soil samples) and studying active diazotrophs. We compared the acetylene reduction assay and the 15N2 tracer method and demonstrated that the latter is more sensitive in samples with low activity. Additionally, tracing 15N into microbial RNA provides much higher sensitivity compared to bulk soil analysis. Active soil diazotrophs were identified with a 15N\uffe2\uff80\uff90RNA\uffe2\uff80\uff90SIP approach optimized for environmental samples and benchmarked to 15N\uffe2\uff80\uff90DNA\uffe2\uff80\uff90SIP. Lastly, we investigated the feasibility of using SIP\uffe2\uff80\uff90Raman microspectroscopy for detecting 15N\uffe2\uff80\uff90labelled cells. Taken together, these tools allow identifying and investigating active free\uffe2\uff80\uff90living diazotrophs in a highly sensitive manner in diverse environments, from bulk to the single\uffe2\uff80\uff90cell level.</p", "keywords": ["Spectrum Analysis", " Raman", "BIOLOGICAL SOIL CRUSTS", "106005 Bioinformatik", "106023 Molekularbiologie", "Nitrogen Fixation", "REVEALS", "FLUORESCENCE", "Research Articles", "Soil Microbiology", "106022 Mikrobiologie", "SPECTROSCOPY", "Bacteria", "Nitrogen Isotopes", "106003 Biodiversity research", "106023 Molecular biology", "GENETIC-REGULATION", "Archaea", "6. Clean water", "SURFACE-ENHANCED RAMAN", "COMMUNITY", "106003 Biodiversit\u00e4tsforschung", "13. Climate action", "Isotope Labeling", "106022 Microbiology", "NITROGEN-FIXATION", "106005 Bioinformatics", "RIBOSOMAL-RNA", "N-2 FIXATION"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13954"}, {"href": "https://doi.org/2764305888"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2764305888", "name": "item", "description": "2764305888", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2764305888"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-15T00:00:00Z"}}, {"id": "PMC5814836", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-25T16:37:18Z", "type": "Journal Article", "created": "2022-10-18", "title": "Application of stable\u2010isotope labelling techniques for the detection of active diazotrophs", "description": "Summary                   <p>                     Investigating active participants in the fixation of dinitrogen gas is vital as N is often a limiting factor for primary production. Biological nitrogen fixation is performed by a diverse guild of bacteria and archaea (diazotrophs), which can be free\uffe2\uff80\uff90living or symbionts. Free\uffe2\uff80\uff90living diazotrophs are widely distributed in the environment, yet our knowledge about their identity and ecophysiology is still limited. A major challenge in investigating this guild is inferring activity from genetic data as this process is highly regulated. To address this challenge, we evaluated and improved several                     15                     N\uffe2\uff80\uff90based methods for detecting N                     2                     fixation activity (with a focus on soil samples) and studying active diazotrophs. We compared the acetylene reduction assay and the                     15                     N                     2                     tracer method and demonstrated that the latter is more sensitive in samples with low activity. Additionally, tracing                     15                     N into microbial RNA provides much higher sensitivity compared to bulk soil analysis. Active soil diazotrophs were identified with a                     15                     N\uffe2\uff80\uff90RNA\uffe2\uff80\uff90SIP approach optimized for environmental samples and benchmarked to                     15                     N\uffe2\uff80\uff90DNA\uffe2\uff80\uff90SIP. Lastly, we investigated the feasibility of using SIP\uffe2\uff80\uff90Raman microspectroscopy for detecting                     15                     N\uffe2\uff80\uff90labelled cells. Taken together, these tools allow identifying and investigating active free\uffe2\uff80\uff90living diazotrophs in a highly sensitive manner in diverse environments, from bulk to the single\uffe2\uff80\uff90cell level.                   </p", "keywords": ["Spectrum Analysis", " Raman", "BIOLOGICAL SOIL CRUSTS", "106005 Bioinformatik", "106023 Molekularbiologie", "Nitrogen Fixation", "REVEALS", "FLUORESCENCE", "Research Articles", "Soil Microbiology", "106022 Mikrobiologie", "SPECTROSCOPY", "Bacteria", "Nitrogen Isotopes", "106003 Biodiversity research", "106023 Molecular biology", "GENETIC-REGULATION", "Archaea", "6. Clean water", "SURFACE-ENHANCED RAMAN", "COMMUNITY", "106003 Biodiversit\u00e4tsforschung", "13. Climate action", "Isotope Labeling", "106022 Microbiology", "NITROGEN-FIXATION", "106005 Bioinformatics", "RIBOSOMAL-RNA", "N-2 FIXATION"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13954"}, {"href": "https://doi.org/PMC5814836"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC5814836", "name": "item", "description": "PMC5814836", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC5814836"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-15T00:00:00Z"}}, {"id": "PMC9579094", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:37:26Z", "type": "Journal Article", "created": "2022-06-22", "title": "Harnessing belowground processes for sustainable intensification of agricultural systems", "description": "Abstract <p>Increasing food demand coupled with climate change pose a great challenge to agricultural systems. In this review we summarize recent advances in our knowledge of how plants, together with their associated microbiota, shape rhizosphere processes. We address (molecular) mechanisms operating at the plant\uffe2\uff80\uff93microbe-soil interface and aim to link this knowledge with actual and potential avenues for intensifying agricultural systems, while at the same time reducing irrigation water, fertilizer inputs and pesticide use. Combining in-depth knowledge about above and belowground plant traits will not only significantly advance our mechanistic understanding of involved processes but also allow for more informed decisions regarding agricultural practices and plant breeding. Including belowground plant-soil-microbe interactions in our breeding efforts will help to select crops resilient to abiotic and biotic environmental stresses and ultimately enable us to produce sufficient food in a more sustainable agriculture in the upcoming decades.</p", "keywords": ["0301 basic medicine", "BIOLOGICAL NITRIFICATION INHIBITION", "PHOSPHATE SOLUBILIZING BACTERIA", "Plant-plant interaction", "Rhizobiome", "MEDIATED PH CHANGES", "Review Article", "Plant health", "MEMBRANE H+-ATPASE", "12. Responsible consumption", "03 medical and health sciences", "Soil health", "Soil structure", "C sequestration", "SDG 13 - Climate Action", "SDG 2 \u2013 Kein Hunger", "106026 Ecosystem research", "Plant nutrition", "SDG 2 - Zero Hunger", "2. Zero hunger", "0303 health sciences", "Water availability", "Root exudation", "JASMONIC ACID", "15. Life on land", "Microbes", "Intercropping", "106026 \u00d6kosystemforschung", "PLANT-GROWTH", "13. Climate action", "ROOT BORDER CELLS", "SDG 13 \u2013 Ma\u00dfnahmen zum Klimaschutz", "C cycling", "INNATE IMMUNITY", "NITROGEN-FIXATION", "ORGANIC-ACIDS"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s11104-022-05508-z.pdf"}, {"href": "https://doi.org/PMC9579094"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC9579094", "name": "item", "description": "PMC9579094", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC9579094"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-22T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=NITROGEN-FIXATION&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=NITROGEN-FIXATION&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=NITROGEN-FIXATION&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=NITROGEN-FIXATION&offset=16", "hreflang": "en-US"}], "numberMatched": 16, "numberReturned": 16, "distributedFeatures": [], "timeStamp": "2026-06-25T22:27:11.626769Z"}