{"type": "FeatureCollection", "features": [{"id": "10.1016/j.ecolind.2020.106669", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:15:26Z", "type": "Journal Article", "created": "2020-07-10", "title": "Optimized crop rotations increase biomass production without significantly changing soil carbon and nitrogen stock", "description": "Abstract   To meet the growing challenges for food security, renewable resource production and climate change adaptation, optimized crop rotations (OCRs) should aim to maximize biomass production and export from the field while minimizing carbon (C) and nitrogen (N) footprints. However, the effects of OCRs on aboveground biomass production and soil C and N stock as well as the potential links between them remain poorly understood. In this study in Denmark, we harvested all aboveground biomass and simultaneously investigated soil C and N content and stock in two continuous monocultures (CMs) as well as in four OCRs. Across five-year continuous observations, OCRs significantly increased cumulative aboveground biomass production by 23% compared to CMs. There was no significant difference between OCRs and CMs in soil C and N content in any of the soil layers (0\u201320, 20\u201350, and 50\u2013100\u00a0cm) after the five years. Moreover, OCRs had no effect on top layer soil C and N stock compared to CMs, even when examined by equivalent soil mass. Slight reductions in soil C and N stock after five years in both OCRs and CMs did not relate to the changes in aboveground biomass production. Our results highlight that it is feasible to produce more biomass for biorefineries in OCRs than in CMs and the reductions in soil C and N stock over time seem similar for the two systems. Longer-term continuous observations are called for to underpin these results.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Optimized crop rotation", "04 agricultural and veterinary sciences", "15. 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However, few studies have concurrently explored changes in biomass yield, yield stability and soil C and N content under different cropping systems targeting biorefinery. In this study, 10 different cropping systems were simultaneously investigated from 2012 to 2017 in central Denmark on a loamy sand soil, including (1) two continuous monocultures of annual crops, (2) one optimized crop rotation, (3) five intensively fertilized perennial grasses, and (4) two grass-legume mixtures without nitrogen (N) fertilization. Our results showed that biomass yield and yield stability differed highly across the cropping systems, highlighting crop-specific characteristics. Of the 10 cropping systems, tall fescue significantly increased soil C and N content at 0\u201320 cm depth, while sustaining high biomass yield and yield stability. There was no clear relationship between biomass yield, yield stability and changes in soil C and N content, challenging some recent findings on the conflicts between increasing biomass yield and protecting soil C and N content. Indeed, the lack of relationships suggest that there is considerable potential to increase biomass yield and yield stability without compromising soil C and N content through selecting proper cropping systems and managements. Altogether, our results underscore how crop-specific documentation of biomass yield, yield stability and changes in soil C and N content on the same experimental platform can advance the understanding of sustainable agriculture for biorefineries, although long-term continuous observations are still required to better clarify the relations between them.</p>", "keywords": ["2. 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However, the effects of OCRs on aboveground biomass production and soil C and N stock as well as the potential links between them remain poorly understood. In this study in Denmark, we harvested all aboveground biomass and simultaneously investigated soil C and N content and stock in two continuous monocultures (CMs) as well as in four OCRs. Across five-year continuous observations, OCRs significantly increased cumulative aboveground biomass production by 23% compared to CMs. There was no significant difference between OCRs and CMs in soil C and N content in any of the soil layers (0\u201320, 20\u201350, and 50\u2013100\u00a0cm) after the five years. Moreover, OCRs had no effect on top layer soil C and N stock compared to CMs, even when examined by equivalent soil mass. Slight reductions in soil C and N stock after five years in both OCRs and CMs did not relate to the changes in aboveground biomass production. 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