{"type": "FeatureCollection", "features": [{"id": "10.1016/j.molp.2024.10.010", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:00Z", "type": "Journal Article", "created": "2024-10-30", "title": "SCOOP10 and SCOOP12 peptides act through MIK2 receptor-like kinase to antagonistically regulate Arabidopsis leaf senescence", "description": "Leaf senescence plays a critical role in a plant's overall reproductive success due to its involvement in nutrient remobilization and allocation. However, our current understanding of the molecular mechanisms controlling leaf senescence remains limited. In this study, we show that the receptor-like kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) functions as a negative regulator of leaf senescence. We found that the SERINE-RICH ENDOGENOUS PEPTIDE 12, previously known to physically interact with MIK2, competes with SCOOP10 to regulate MIK2-dependent leaf senescence. We observed that increased expression of SCOOP10 or the application of exogenous SCOOP10 peptides accelerated leaf senescence in a MIK2-dependent manner. Conversely, SCOOP12 acted as a suppressor of MIK2-dependent leaf senescence regulation. Biochemical assays showed that SCOOP12 enhances while SCOOP10 diminishes MIK2 phosphorylation. Thus, the SCOOP12-MIK2 module might function antagonistically on SCOOP10-MIK2 signaling at late senescing stages, allowing for fine-tuned modulation of the leaf senescence process. Our study sheds light on the complex mechanisms underlying leaf senescence and provides valuable insights into the interplay between receptors, peptides, and the regulation of plant senescence.", "keywords": ["leaf senescence", "Arabidopsis Proteins", "Arabidopsis", "Protein Serine-Threonine Kinases", "Plant Senescence", "receptor-like kinase", "Plant Leaves", "SCOOP12", "Gene Expression Regulation", " Plant", "small signaling peptides", "MIK2", "Phosphorylation", "SCOOP10", "Peptides", "Protein Kinases", "Research Article"]}, "links": [{"href": "https://doi.org/10.1016/j.molp.2024.10.010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Molecular%20Plant", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.molp.2024.10.010", "name": "item", "description": "10.1016/j.molp.2024.10.010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.molp.2024.10.010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.1016/j.pbi.2017.05.005", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:01Z", "type": "Journal Article", "created": "2017-06-03", "title": "Small peptide signaling pathways modulating macronutrient utilization in plants", "description": "Root system architecture (RSA) and physiological functions define macronutrient uptake efficiency. Small signaling peptides (SSPs), that act in manners similar to hormones, and their cognate receptors transmit signals both locally and systemically. Several SSPs controlling morphological and physiological traits of roots have been identified to be associated with macronutrient uptake. Recent development in plant genome research has provided an avenue toward systems-based identification and prediction of additional SSPs. This review highlights recent studies on SSP pathways important for optimization of macronutrient uptake and provides new insights into the diversity of SSPs regulated in response to changes in macronutrient availabilities.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Nitrogen", "Gene Expression", "Plants", "Peptides", "Plant Root Nodulation", "Plant Roots", "Signal Transduction"]}, "links": [{"href": "https://doi.org/10.1016/j.pbi.2017.05.005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Current%20Opinion%20in%20Plant%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.pbi.2017.05.005", "name": "item", "description": "10.1016/j.pbi.2017.05.005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.pbi.2017.05.005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-10-01T00:00:00Z"}}, {"id": "10.1016/j.peptides.2021.170696", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:01Z", "type": "Journal Article", "created": "2021-11-29", "title": "Immunomodulatory peptides\u2014A promising source for novel functional food production and drug discovery", "description": "Immunomodulatory peptides are a complex class of bioactive peptides that encompasses substances with different mechanisms of action. Immunomodulatory peptides could also be used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics. Thus, immunomodulatory peptides in food of plant origin could be regarded both as valuable suplements of novel functional food preparation and/or as precursors or possible active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system. Given variety of mechanisms, different tests are required to assess effects of immunomodulatory peptides. Some of those effects show good correlation with in vivo results but others, less so. Certain plant peptides, such as defensins, show both immunomodulatory and antimicrobial effect, which makes them interesting candidates for preparation of functional food and feed, as well as templates for design of synthetic peptides.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Vaccines", "570", "0303 health sciences", "Immunomodulatory peptides  Defensin  Cryptic peptide  Functional food  Mechanism of action  Vaccines", "Functional food", "Cryptic peptide", "610", "Mechanism of action", "3. Good health", "Defensins", "Immunomodulation", "03 medical and health sciences", "Immunomodulatory peptide", "Defensin", "Functional Food", "Drug Discovery", "Humans", "Peptides", "Settore BIOS-10/A - Biologia cellulare e applicata", "Immunomodulatory peptides Defensin Cryptic peptide Functional food Mechanism of action Vaccines", "Plant Proteins"]}, "links": [{"href": "https://doi.org/10.1016/j.peptides.2021.170696"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Peptides", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.peptides.2021.170696", "name": "item", "description": "10.1016/j.peptides.2021.170696", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.peptides.2021.170696"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "10.1056/nejmoa2212825", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:57Z", "type": "Journal Article", "created": "2023-05-17", "title": "Emodepside for             Trichuris trichiura             and Hookworm Infection", "description": "Current treatments for soil-transmitted helminth infections in humans have low efficacy against Trichuris trichiura. Emodepside - a drug in veterinary use and under development for the treatment of onchocerciasis in humans - is a leading therapeutic candidate for soil-transmitted helminth infection.We conducted two phase 2a, dose-ranging, randomized, controlled trials to evaluate the efficacy and safety of emodepside against T. trichiura and hookworm infections. We randomly assigned, in equal numbers, adults 18 to 45 years of age in whom T. trichiura or hookworm eggs had been detected in stool samples to receive emodepside, at a single oral dose of 5, 10, 15, 20, 25, or 30 mg; albendazole, at a single oral dose of 400 mg; or placebo. The primary outcome was the percentage of participants who were cured of T. trichiura or hookworm infection (the cure rate) with emodepside 14 to 21 days after treatment, determined with the use of the Kato-Katz thick-smear technique. Safety was assessed 3, 24, and 48 hours after the receipt of treatment or placebo.A total of 266 persons were enrolled in the T. trichiura trial and 176 in the hookworm trial. The predicted cure rate against T. trichiura in the 5-mg emodepside group (85% [95% confidence interval {CI}, 69 to 93]; 25 of 30 participants) was higher than the predicted cure rate in the placebo group (10% [95% CI, 3 to 26]; 3 of 31 participants) and the observed cure rate in the albendazole group (17% [95% CI, 6 to 35]; 5 of 30 participants). A dose-dependent relationship was shown in participants with hookworm: the observed cure rate was 32% (95% CI, 13 to 57; 6 of 19 participants) in the 5-mg emodepside group and 95% (95% CI, 74 to 99.9; 18 of 19 participants) in the 30-mg emodepside group; the observed cure rates were 14% (95% CI, 3 to 36; 3 of 21 participants) in the placebo group and 70% (95% CI, 46 to 88; 14 of 20 participants) in the albendazole group. In the emodepside groups, headache, blurred vision, and dizziness were the most commonly reported adverse events 3 and 24 hours after treatment; the incidence of events generally increased in a dose-dependent fashion. Most adverse events were mild in severity and were self-limited; there were few moderate and no serious adverse events.Emodepside showed activity against T. trichiura and hookworm infections. (Funded by the European Research Council; ClinicalTrials.gov number, NCT05017194.).", "keywords": ["Adult", "Dose-Response Relationship", " Drug", "Antinematodal Agents", "Administration", " Oral", "Middle Aged", "Albendazole", "16. Peace & justice", "3. Good health", "03 medical and health sciences", "Feces", "Hookworm Infections", "Soil", "Young Adult", "0302 clinical medicine", "Trichuris", "Depsipeptides", "Animals", "Humans", "Trichuriasis"]}, "links": [{"href": "https://doi.org/10.1056/nejmoa2212825"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20England%20Journal%20of%20Medicine", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1056/nejmoa2212825", "name": "item", "description": "10.1056/nejmoa2212825", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1056/nejmoa2212825"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-05-18T00:00:00Z"}}, {"id": "10.1038/s41477-023-01583-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:46Z", "type": "Journal Article", "created": "2023-12-04", "title": "Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES", "description": "Plant signalling peptides are typically released from larger precursors by proteolytic cleavage to regulate plant growth, development and stress responses. Recent studies reported the characterization of a divergent family of Brassicaceae-specific peptides, SERINE RICH ENDOGENOUS PEPTIDES (SCOOPs), and their perception by the leucine-rich repeat receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2). Here, we reveal that the SCOOP family is highly expanded, containing at least 50 members in the Columbia-0 reference Arabidopsis thaliana genome. Notably, perception of these peptides is strictly MIK2-dependent. How bioactive SCOOP peptides are produced, and to what extent their perception is responsible for the multiple physiological roles associated with MIK2 are currently unclear. Using N-terminomics, we validate the N-terminal cleavage site of representative PROSCOOPs. The cleavage sites are determined by conserved motifs upstream of the minimal SCOOP bioactive epitope. We identified subtilases necessary and sufficient to process PROSCOOP peptides at conserved cleavage motifs. Mutation of these subtilases, or their recognition motifs, suppressed PROSCOOP cleavage and associated overexpression phenotypes. Furthermore, we show that higher-order mutants of these subtilases show phenotypes reminiscent of mik2 null mutant plants, consistent with impaired PROSCOOP biogenesis, and demonstrating biological relevance of SCOOP perception by MIK2. Together, this work provides insights into the molecular mechanisms underlying the functions of the recently identified SCOOP peptides and their receptor MIK2.", "keywords": ["570", "Arabidopsis Proteins", "[SDV]Life Sciences [q-bio]", "Arabidopsis", "Receptors", " Cell Surface", "580 Plants (Botany)", "[SDV] Life Sciences [q-bio]", "10126 Department of Plant and Microbial Biology", "1110 Plant Science", "Taverne", "Brassicaceae", "Serine", "Life Science", "10211 Zurich-Basel Plant Science Center", "Peptides", "Protein Kinases"]}, "links": [{"href": "https://www.nature.com/articles/s41477-023-01583-x.pdf"}, {"href": "https://hal.science/hal-04394015/file/NaturePlants_subtilase_scoop_2023_revised.pdf"}, {"href": "https://doi.org/10.1038/s41477-023-01583-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Plants", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41477-023-01583-x", "name": "item", "description": "10.1038/s41477-023-01583-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41477-023-01583-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-04T00:00:00Z"}}, {"id": "10.1038/s41467-018-04594-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:45Z", "type": "Journal Article", "created": "2018-05-29", "title": "Resolving molecule-specific information in dynamic lipid membrane processes with multi-resonant infrared metasurfaces", "description": "Abstract<p>A multitude of biological processes are enabled by complex interactions between lipid membranes and proteins. To understand such dynamic processes, it is crucial to differentiate the constituent biomolecular species and track their individual time evolution without invasive labels. Here, we present a label-free mid-infrared biosensor capable of distinguishing multiple analytes in heterogeneous biological samples with high sensitivity. Our technology leverages a multi-resonant metasurface to simultaneously enhance the different vibrational fingerprints of multiple biomolecules. By providing up to 1000-fold near-field intensity enhancement over both amide and methylene bands, our sensor resolves the interactions of lipid membranes with different polypeptides in real time. Significantly, we demonstrate that our label-free chemically specific sensor can analyze peptide-induced neurotransmitter cargo release from synaptic vesicle mimics. Our sensor opens up exciting possibilities for gaining new insights into biological processes such as signaling or transport in basic research as well as provides a valuable toolkit for bioanalytical and pharmaceutical applications.</p", "keywords": ["Science", "Circular Dichroism", "Q", "Lipid Bilayers", "Membrane Proteins", "Reproducibility of Results", "Biosensing Techniques", "02 engineering and technology", "01 natural sciences", "Article", "0104 chemical sciences", "Membrane Lipids", "Spectroscopy", " Fourier Transform Infrared", "Peptides", "0210 nano-technology", "Protein Binding"]}, "links": [{"href": "https://eprints.gla.ac.uk/164038/1/164038.pdf"}, {"href": "https://www.nature.com/articles/s41467-018-04594-x.pdf"}, {"href": "https://doi.org/10.1038/s41467-018-04594-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-018-04594-x", "name": "item", "description": "10.1038/s41467-018-04594-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-018-04594-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-06-04T00:00:00Z"}}, {"id": "10.1038/s41598-023-49194-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:49Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "Abstract<p>Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.</p", "keywords": ["0301 basic medicine", "Bacillus", "Gene", "Agricultural and Biological Sciences", "https://purl.org/becyt/ford/1.6", "Phylogeny", "GC-content", "2. Zero hunger", "0303 health sciences", "Genome", "Acinetobacter", "soil bacteria", "Q", "Probiotics and Prebiotics", "R", "Life Sciences", "Anti-Bacterial Agents", "3. Good health", "Ribosomal RNA", "Medicine", "Microbial genetics", "metagenomics assembly", "Biotechnology", "Bacteriocin", "Science", ".", "Synteny", "Microbiology", "Article", "Applied microbiology", "Lipopeptides", "03 medical and health sciences", "Biochemistry", " Genetics and Molecular Biology", "Genetics", "Escherichia coli", "RNA Sequencing Data Analysis", "https://purl.org/becyt/ford/1", "Molecular Biology", "Biology", "genetic engineering", "Bacteria", "Secondary metabolites", "In silico", "bacterial genomes", "Whole genome sequencing", "FOS: Biological sciences", "Microbial Enzymes and Biotechnological Applications", "Antibacterial activity", "Genome", " Bacterial", "Food Science", "Phylogenetic tree"]}, "links": [{"href": "https://www.nature.com/articles/s41598-023-49194-y.pdf"}, {"href": "https://doi.org/10.1038/s41598-023-49194-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-023-49194-y", "name": "item", "description": "10.1038/s41598-023-49194-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-023-49194-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-13T00:00:00Z"}}, {"id": "10.1371/journal.pntd.0012872", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:18:15Z", "type": "Journal Article", "created": "2025-02-18", "title": "Performance of real-time polymerase chain reaction and Kato-Katz for diagnosing soil-transmitted helminth infections and evaluating treatment efficacy of emodepside in randomized controlled trials", "description": "Background <p>The World Health Organization recommends the use of the microscopy-based Kato-Katz thick smear for diagnosing soil-transmitted helminth (STH) infections. Despite its simplicity and cost-effectiveness, the Kato-Katz method faces challenges, including reader subjectivity and reduced sensitivity. Real-time polymerase chain reaction (qPCR) technology offers standardized readouts and higher sensitivity, making it suitable for STH diagnosis and monitoring the treatment efficacy of emodepside within the framework of randomized controlled trials.</p>   Methodology/Principal findings <p>We evaluated the performance of Kato-Katz versus qPCR for assessing treatment efficacy in terms of cure rates, of single doses of 5, 10, 15, 20, 25 and 30\uffe2\uff80\uff89mg of emodepside compared to 400\uffe2\uff80\uff89mg albendazole. Spearman\uffe2\uff80\uff99s rank correlation coefficient examined the correlation between STH eggs per gram in stool samples and qPCR Ct values. Diagnostic sensitivity of qPCR was calculated using a Bayesian latent class modelling approach with data from Ascaris lumbricoides infections. Agreement between Kato-Katz and qPCR at baseline was 93.57% for Trichuris trichiura, and 73.49% for both hookworm and A. lumbricoides. For the latter helminth qPCR demonstrated higher sensitivity (85.00% vs. 47.70%) and slightly lower specificity (93.40% vs. 99.40%) compared to Kato-Katz. We observed a fair to moderate agreement with negative correlation between Ct values and Kato-Katz egg counts. Treatment efficacy, as assessed by qPCR, was lower for all doses of emodepside and albendazole compared to Kato-Katz. Nonetheless, emodepside demonstrated higher cure rates against T. trichiura and A. lumbricoides infections compared to albendazole.</p>   Conclusion/ Significance <p>Our study confirmed that qPCR is a sensitive diagnostic method for diagnosing STH infections compared to Kato-Katz and serves as a valuable tool for determining treatment efficacy in clinical trials. Furthermore, qPCR confirmed the better treatment efficacy of emodepside compared to albendazole, despite indicating lower cure rates than Kato-Katz.</p", "keywords": ["Anthelmintics", "Male", "Adult", "Adolescent", "RC955-962", "Helminthiasis", "Real-Time Polymerase Chain Reaction", "Albendazole", "Sensitivity and Specificity", "Soil", "Feces", "Young Adult", "Treatment Outcome", "Trichuris", "Arctic medicine. Tropical medicine", "Depsipeptides", "Humans", "Animals", "Female", "Public aspects of medicine", "RA1-1270", "Child", "Ascaris lumbricoides", "Parasite Egg Count", "Research Article"], "contacts": [{"organization": "Christian N. Lotz, Emmanuel C. Mrimi, Pierre H. H. Schneeberger, Said M. Ali, Jan Hattendorf, Jennifer Keiser,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1371/journal.pntd.0012872"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20Neglected%20Tropical%20Diseases", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pntd.0012872", "name": "item", "description": "10.1371/journal.pntd.0012872", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pntd.0012872"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-18T00:00:00Z"}}, {"id": "10.1201/9781003106524-10", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:18:10Z", "type": "Report", "created": "2022-01-21", "title": "Bioactive Peptides in Pulses", "description": "Pulse proteins are a source of a range of biologically active substances which are inactive within the sequence of native protein but can be released by enzymatic hydrolysis, fermentation, or germination. Pulse-borne bioactive peptides have been found to exhibit various in vitro activities, such as antihypertensive, antioxidant, anticholesterolemic, antidiabetic, and antimicrobial. In addition, several peptides have multifunction properties, especially antihypertensive and antioxidant. Such bioactive peptides may find use in the treatment of hypertension, diabetes, high cholesterol, and oxidative stress. They can be also applied as natural ingredients in food products to increase shelf life. These bioactive peptides may be used in the formulation of functional foods, nutraceuticals, and natural drugs because of their health benefit effects.", "keywords": ["2. Zero hunger", "peptides", "610", "fermentation (alteration)", "health benefits", "3. Good health"]}, "links": [{"href": "https://doi.org/10.1201/9781003106524-10"}, {"rel": "self", "type": "application/geo+json", "title": "10.1201/9781003106524-10", "name": "item", "description": "10.1201/9781003106524-10", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1201/9781003106524-10"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-21T00:00:00Z"}}, {"id": "11828476", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:22:21Z", "type": "Journal Article", "created": "2002-08-25", "title": "The Outstanding Biological Stability of - and -Peptides toward Proteolytic Enzymes: An In Vitro Investigation with Fifteen Peptidases", "description": "A series of 36 linear and cyclic beta- and gamma-peptides consisting of as few as two, and as many as 15 residues, was offered as substrates to 15 commercially available proteases of bacterial, fungal, and eukaryotic origin, including a beta-lactamase and amidases, as well as most vigorous, nonspecific proteases, such as the 20S proteasome from human erythrocytes. For comparison, an alpha-eicosapeptide and standard substrates of the proteolytic enzymes were included in the investigation. Under conditions of complete cleavage of the alpha-peptide within 15 min the beta- and gamma-peptides were stable for at least 48 h. Inhibition studies with seven beta- and gamma-peptides and alpha-chymotrypsin show that the residual enzyme activity toward succinyl-Ala-Ala-Pro-Phe-p-nitroanilide is unchanged within experimental error after incubation for 15 min with the peptide analogues. Thus, beta- and gamma-peptides with proteinogenic side chains, that is, consisting of the singly or doubly homologated natural alpha-amino acids (one or two CH(2) groups inserted in the backbone of each residue), are completely stable to common proteases, without inhibiting their normal activity (as demonstrated for alpha-chymotrypsin). This proteolytic stability of peptides built of homologated amino acids is a prerequisite for their potential use as drugs.", "keywords": ["0301 basic medicine", "0303 health sciences", "Molecular Structure", "Peptides", " Cyclic", "Protein Structure", " Tertiary", "3. Good health", "Fungal Proteins", "03 medical and health sciences", "Bacterial Proteins", "Catalytic Domain", "Humans", "Enzyme Inhibitors", "Peptides", "Chromatography", " High Pressure Liquid", "Peptide Hydrolases", "Protein Binding"]}, "links": [{"href": "https://doi.org/11828476"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ChemBioChem", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11828476", "name": "item", "description": "11828476", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11828476"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2001-06-01T00:00:00Z"}}, {"id": "11381/2905288", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:22:17Z", "type": "Journal Article", "created": "2021-11-29", "title": "Immunomodulatory peptides\u2014A promising source for novel functional food production and drug discovery", "description": "Immunomodulatory peptides are a complex class of bioactive peptides that encompasses substances with different mechanisms of action. Immunomodulatory peptides could also be used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics. Thus, immunomodulatory peptides in food of plant origin could be regarded both as valuable suplements of novel functional food preparation and/or as precursors or possible active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system. Given variety of mechanisms, different tests are required to assess effects of immunomodulatory peptides. Some of those effects show good correlation with in vivo results but others, less so. Certain plant peptides, such as defensins, show both immunomodulatory and antimicrobial effect, which makes them interesting candidates for preparation of functional food and feed, as well as templates for design of synthetic peptides.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Vaccines", "570", "0303 health sciences", "Functional food", "Cryptic peptide", "610", "Mechanism of action", "3. Good health", "Defensins", "Immunomodulation", "03 medical and health sciences", "Immunomodulatory peptide", "Defensin", "Functional Food", "Drug Discovery", "Humans", "Peptides", "Settore BIOS-10/A - Biologia cellulare e applicata", "Plant Proteins"]}, "links": [{"href": "https://doi.org/11381/2905288"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Peptides", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11381/2905288", "name": "item", "description": "11381/2905288", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/2905288"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "11381/2937853", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-09-21T16:22:17Z", "type": "Report", "created": "2022-01-21", "title": "Bioactive Peptides in Pulses", "description": "Pulse proteins are a source of a range of biologically active substances which are inactive within the sequence of native protein but can be released by enzymatic hydrolysis, fermentation, or germination. Pulse-borne bioactive peptides have been found to exhibit various in vitro activities, such as antihypertensive, antioxidant, anticholesterolemic, antidiabetic, and antimicrobial. In addition, several peptides have multifunction properties, especially antihypertensive and antioxidant. Such bioactive peptides may find use in the treatment of hypertension, diabetes, high cholesterol, and oxidative stress. They can be also applied as natural ingredients in food products to increase shelf life. These bioactive peptides may be used in the formulation of functional foods, nutraceuticals, and natural drugs because of their health benefit effects.", "keywords": ["2. Zero hunger", "peptides", "610", "fermentation (alteration)", "health benefits", "3. Good health"]}, "links": [{"href": "https://doi.org/11381/2937853"}, {"rel": "self", "type": "application/geo+json", "title": "11381/2937853", "name": "item", "description": "11381/2937853", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/2937853"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-21T00:00:00Z"}}, {"id": "11336/226991", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:22:16Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "Abstract<p>Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.</p", "keywords": ["0301 basic medicine", "Bacteriocin", "Science", "Bacillus", ".", "Gene", "Synteny", "Microbiology", "Article", "Agricultural and Biological Sciences", "Lipopeptides", "03 medical and health sciences", "https://purl.org/becyt/ford/1.6", "Biochemistry", " Genetics and Molecular Biology", "Genetics", "Escherichia coli", "RNA Sequencing Data Analysis", "https://purl.org/becyt/ford/1", "Molecular Biology", "Biology", "Phylogeny", "GC-content", "2. Zero hunger", "0303 health sciences", "Genome", "Acinetobacter", "Bacteria", "Secondary metabolites", "Q", "Probiotics and Prebiotics", "In silico", "R", "Life Sciences", "Anti-Bacterial Agents", "3. Good health", "Ribosomal RNA", "Whole genome sequencing", "FOS: Biological sciences", "Medicine", "Microbial Enzymes and Biotechnological Applications", "Antibacterial activity", "Genome", " Bacterial", "metagenomics assembly", "Biotechnology", "Food Science", "Phylogenetic tree"]}, "links": [{"href": "https://www.nature.com/articles/s41598-023-49194-y.pdf"}, {"href": "https://doi.org/11336/226991"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11336/226991", "name": "item", "description": "11336/226991", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11336/226991"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-13T00:00:00Z"}}, {"id": "13946230", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:22:23Z", "type": "Journal Article", "created": "2013-06-11", "title": "Observations on Mechanism of Action of the Antifungal Peptide, Ro2-7758.", "description": "SummaryInhibitory action of the antifungal sulfur-containing peptide, Ro2-7758 (formerly X-5079C), against the growth of Mucor corymbifera was annulled by sulfite and cysteine, but not by sulfate or 96 other substances tested. Experiments with S35O4 showed that the drug had no effect on permeability of yeast cells to sulfate. However, production of S35O3 from S35O4 by a cell-free sulfate reductase system from yeast was first stimulated at low drug concentrations and then inhibited at higher concentrations. It was concluded that this action of the drug on sulfate reductase may be involved in its antibiotic activity.", "keywords": ["Antifungal Agents", "Mucor", "Sulfites", "Cysteine", "Peptides", "Fungicides", " Industrial", "3. Good health"], "contacts": [{"organization": "G R, GALE, S M, KENDALL, A M, WELCH,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/13946230"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Experimental%20Biology%20and%20Medicine", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "13946230", "name": "item", "description": "13946230", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/13946230"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1963-05-01T00:00:00Z"}}, {"id": "2433/277223", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-09-21T16:22:49Z", "type": "Journal Article", "created": "2022-10-18", "title": "Development of Antimicrobial Peptide\u2013Antibiotic Conjugates to Improve the Outer Membrane Permeability of Antibiotics Against Gram-Negative Bacteria", "description": "Antibiotics have been widely used in the medical field as a treatment for infectious diseases, but they are not effective against all Gram-negative bacteria because of their low permeability to the outer membrane. One of the strategies to improve the antibacterial activity of antibiotics is the coadministration of antibiotics and membrane-perturbing antimicrobial peptides for their synergistic effects. However, because of their different pharmacokinetics, their coadministration may not exert expected effects in the clinical stage. Here, we designed various antimicrobial peptide-antibiotic conjugates as a novel approach to improve the antimicrobial activity of antibiotics. Ampicillin was chosen as a model antibiotic with poor outer membrane permeability, and the effects of the chemistry and position of conjugation and the choice of antimicrobial peptides were examined. One of the ampicillin conjugates exhibited significantly improved antimicrobial activity against ampicillin-resistant Gram-negative bacteria without exerting cytotoxicity against human cultured cells, demonstrating that our novel approach is an effective strategy to improve the antimicrobial activity of antibiotics with low outer membrane permeability.", "keywords": ["0301 basic medicine", "antimicrobial peptide", "Bacteria", "Microbial Sensitivity Tests", "Permeability", "Anti-Bacterial Agents", "3. Good health", "Gram-negative bacterium", "03 medical and health sciences", "conjugate", "membrane permeability", "Gram-Negative Bacteria", "ampicillin", "Humans", "disulfide bond", "Ampicillin", "Antimicrobial Peptides"]}, "links": [{"href": "https://doi.org/2433/277223"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ACS%20Infectious%20Diseases", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2433/277223", "name": "item", "description": "2433/277223", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2433/277223"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-10-18T00:00:00Z"}}, {"id": "2805867715", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:22:54Z", "type": "Journal Article", "created": "2018-05-29", "title": "Resolving molecule-specific information in dynamic lipid membrane processes with multi-resonant infrared metasurfaces", "description": "Abstract<p>A multitude of biological processes are enabled by complex interactions between lipid membranes and proteins. To understand such dynamic processes, it is crucial to differentiate the constituent biomolecular species and track their individual time evolution without invasive labels. Here, we present a label-free mid-infrared biosensor capable of distinguishing multiple analytes in heterogeneous biological samples with high sensitivity. Our technology leverages a multi-resonant metasurface to simultaneously enhance the different vibrational fingerprints of multiple biomolecules. By providing up to 1000-fold near-field intensity enhancement over both amide and methylene bands, our sensor resolves the interactions of lipid membranes with different polypeptides in real time. Significantly, we demonstrate that our label-free chemically specific sensor can analyze peptide-induced neurotransmitter cargo release from synaptic vesicle mimics. Our sensor opens up exciting possibilities for gaining new insights into biological processes such as signaling or transport in basic research as well as provides a valuable toolkit for bioanalytical and pharmaceutical applications.</p", "keywords": ["Science", "Circular Dichroism", "Q", "Lipid Bilayers", "Membrane Proteins", "Reproducibility of Results", "Biosensing Techniques", "02 engineering and technology", "01 natural sciences", "Article", "0104 chemical sciences", "Membrane Lipids", "Spectroscopy", " Fourier Transform Infrared", "Peptides", "0210 nano-technology", "Protein Binding"]}, "links": [{"href": "https://eprints.gla.ac.uk/164038/1/164038.pdf"}, {"href": "https://www.nature.com/articles/s41467-018-04594-x.pdf"}, {"href": "https://doi.org/2805867715"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2805867715", "name": "item", "description": "2805867715", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2805867715"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-06-04T00:00:00Z"}}, {"id": "37195942", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:23:22Z", "type": "Journal Article", "created": "2023-05-17", "title": "Emodepside for             Trichuris trichiura             and Hookworm Infection", "description": "Current treatments for soil-transmitted helminth infections in humans have low efficacy against Trichuris trichiura. Emodepside - a drug in veterinary use and under development for the treatment of onchocerciasis in humans - is a leading therapeutic candidate for soil-transmitted helminth infection.We conducted two phase 2a, dose-ranging, randomized, controlled trials to evaluate the efficacy and safety of emodepside against T. trichiura and hookworm infections. We randomly assigned, in equal numbers, adults 18 to 45 years of age in whom T. trichiura or hookworm eggs had been detected in stool samples to receive emodepside, at a single oral dose of 5, 10, 15, 20, 25, or 30 mg; albendazole, at a single oral dose of 400 mg; or placebo. The primary outcome was the percentage of participants who were cured of T. trichiura or hookworm infection (the cure rate) with emodepside 14 to 21 days after treatment, determined with the use of the Kato-Katz thick-smear technique. Safety was assessed 3, 24, and 48 hours after the receipt of treatment or placebo.A total of 266 persons were enrolled in the T. trichiura trial and 176 in the hookworm trial. The predicted cure rate against T. trichiura in the 5-mg emodepside group (85% [95% confidence interval {CI}, 69 to 93]; 25 of 30 participants) was higher than the predicted cure rate in the placebo group (10% [95% CI, 3 to 26]; 3 of 31 participants) and the observed cure rate in the albendazole group (17% [95% CI, 6 to 35]; 5 of 30 participants). A dose-dependent relationship was shown in participants with hookworm: the observed cure rate was 32% (95% CI, 13 to 57; 6 of 19 participants) in the 5-mg emodepside group and 95% (95% CI, 74 to 99.9; 18 of 19 participants) in the 30-mg emodepside group; the observed cure rates were 14% (95% CI, 3 to 36; 3 of 21 participants) in the placebo group and 70% (95% CI, 46 to 88; 14 of 20 participants) in the albendazole group. In the emodepside groups, headache, blurred vision, and dizziness were the most commonly reported adverse events 3 and 24 hours after treatment; the incidence of events generally increased in a dose-dependent fashion. Most adverse events were mild in severity and were self-limited; there were few moderate and no serious adverse events.Emodepside showed activity against T. trichiura and hookworm infections. (Funded by the European Research Council; ClinicalTrials.gov number, NCT05017194.).", "keywords": ["Adult", "Dose-Response Relationship", " Drug", "Antinematodal Agents", "Administration", " Oral", "Middle Aged", "Albendazole", "16. Peace & justice", "3. Good health", "03 medical and health sciences", "Feces", "Hookworm Infections", "Soil", "Young Adult", "0302 clinical medicine", "Trichuris", "Depsipeptides", "Animals", "Humans", "Trichuriasis"]}, "links": [{"href": "https://doi.org/37195942"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20England%20Journal%20of%20Medicine", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "37195942", "name": "item", "description": "37195942", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/37195942"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-05-18T00:00:00Z"}}, {"id": "38049516", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:23:23Z", "type": "Journal Article", "created": "2023-12-04", "title": "Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES", "description": "Plant signalling peptides are typically released from larger precursors by proteolytic cleavage to regulate plant growth, development and stress responses. Recent studies reported the characterization of a divergent family of Brassicaceae-specific peptides, SERINE RICH ENDOGENOUS PEPTIDES (SCOOPs), and their perception by the leucine-rich repeat receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2). Here, we reveal that the SCOOP family is highly expanded, containing at least 50 members in the Columbia-0 reference Arabidopsis thaliana genome. Notably, perception of these peptides is strictly MIK2-dependent. How bioactive SCOOP peptides are produced, and to what extent their perception is responsible for the multiple physiological roles associated with MIK2 are currently unclear. Using N-terminomics, we validate the N-terminal cleavage site of representative PROSCOOPs. The cleavage sites are determined by conserved motifs upstream of the minimal SCOOP bioactive epitope. We identified subtilases necessary and sufficient to process PROSCOOP peptides at conserved cleavage motifs. Mutation of these subtilases, or their recognition motifs, suppressed PROSCOOP cleavage and associated overexpression phenotypes. Furthermore, we show that higher-order mutants of these subtilases show phenotypes reminiscent of mik2 null mutant plants, consistent with impaired PROSCOOP biogenesis, and demonstrating biological relevance of SCOOP perception by MIK2. Together, this work provides insights into the molecular mechanisms underlying the functions of the recently identified SCOOP peptides and their receptor MIK2.", "keywords": ["570", "Arabidopsis Proteins", "[SDV]Life Sciences [q-bio]", "Arabidopsis", "Receptors", " Cell Surface", "580 Plants (Botany)", "[SDV] Life Sciences [q-bio]", "10126 Department of Plant and Microbial Biology", "1110 Plant Science", "Taverne", "Brassicaceae", "Serine", "Life Science", "10211 Zurich-Basel Plant Science Center", "Peptides", "Protein Kinases"]}, "links": [{"href": "https://www.nature.com/articles/s41477-023-01583-x.pdf"}, {"href": "https://hal.science/hal-04394015/file/NaturePlants_subtilase_scoop_2023_revised.pdf"}, {"href": "https://doi.org/38049516"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Plants", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "38049516", "name": "item", "description": "38049516", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/38049516"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-04T00:00:00Z"}}, {"id": "PMC11630628", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:24:39Z", "type": "Journal Article", "created": "2024-10-30", "title": "SCOOP10 and SCOOP12 peptides act through MIK2 receptor-like kinase to antagonistically regulate Arabidopsis leaf senescence", "description": "Leaf senescence plays a critical role in a plant's overall reproductive success due to its involvement in nutrient remobilization and allocation. However, our current understanding of the molecular mechanisms controlling leaf senescence remains limited. In this study, we show that the receptor-like kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) functions as a negative regulator of leaf senescence. We found that the SERINE-RICH ENDOGENOUS PEPTIDE 12, previously known to physically interact with MIK2, competes with SCOOP10 to regulate MIK2-dependent leaf senescence. We observed that increased expression of SCOOP10 or the application of exogenous SCOOP10 peptides accelerated leaf senescence in a MIK2-dependent manner. Conversely, SCOOP12 acted as a suppressor of MIK2-dependent leaf senescence regulation. Biochemical assays showed that SCOOP12 enhances while SCOOP10 diminishes MIK2 phosphorylation. Thus, the SCOOP12-MIK2 module might function antagonistically on SCOOP10-MIK2 signaling at late senescing stages, allowing for fine-tuned modulation of the leaf senescence process. Our study sheds light on the complex mechanisms underlying leaf senescence and provides valuable insights into the interplay between receptors, peptides, and the regulation of plant senescence.", "keywords": ["leaf senescence", "Arabidopsis Proteins", "Arabidopsis", "Protein Serine-Threonine Kinases", "Plant Senescence", "receptor-like kinase", "Plant Leaves", "SCOOP12", "Gene Expression Regulation", " Plant", "small signaling peptides", "MIK2", "Phosphorylation", "SCOOP10", "Peptides", "Protein Kinases", "Research Article"]}, "links": [{"href": "https://doi.org/PMC11630628"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Molecular%20Plant", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11630628", "name": "item", "description": "PMC11630628", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11630628"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "PMC11835329", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:24:39Z", "type": "Journal Article", "created": "2025-02-18", "title": "Performance of real-time polymerase chain reaction and Kato-Katz for diagnosing soil-transmitted helminth infections and evaluating treatment efficacy of emodepside in randomized controlled trials", "description": "Background <p>The World Health Organization recommends the use of the microscopy-based Kato-Katz thick smear for diagnosing soil-transmitted helminth (STH) infections. Despite its simplicity and cost-effectiveness, the Kato-Katz method faces challenges, including reader subjectivity and reduced sensitivity. Real-time polymerase chain reaction (qPCR) technology offers standardized readouts and higher sensitivity, making it suitable for STH diagnosis and monitoring the treatment efficacy of emodepside within the framework of randomized controlled trials.</p>   Methodology/Principal findings <p>We evaluated the performance of Kato-Katz versus qPCR for assessing treatment efficacy in terms of cure rates, of single doses of 5, 10, 15, 20, 25 and 30\uffe2\uff80\uff89mg of emodepside compared to 400\uffe2\uff80\uff89mg albendazole. Spearman\uffe2\uff80\uff99s rank correlation coefficient examined the correlation between STH eggs per gram in stool samples and qPCR Ct values. Diagnostic sensitivity of qPCR was calculated using a Bayesian latent class modelling approach with data from Ascaris lumbricoides infections. Agreement between Kato-Katz and qPCR at baseline was 93.57% for Trichuris trichiura, and 73.49% for both hookworm and A. lumbricoides. For the latter helminth qPCR demonstrated higher sensitivity (85.00% vs. 47.70%) and slightly lower specificity (93.40% vs. 99.40%) compared to Kato-Katz. We observed a fair to moderate agreement with negative correlation between Ct values and Kato-Katz egg counts. Treatment efficacy, as assessed by qPCR, was lower for all doses of emodepside and albendazole compared to Kato-Katz. Nonetheless, emodepside demonstrated higher cure rates against T. trichiura and A. lumbricoides infections compared to albendazole.</p>   Conclusion/ Significance <p>Our study confirmed that qPCR is a sensitive diagnostic method for diagnosing STH infections compared to Kato-Katz and serves as a valuable tool for determining treatment efficacy in clinical trials. Furthermore, qPCR confirmed the better treatment efficacy of emodepside compared to albendazole, despite indicating lower cure rates than Kato-Katz.</p", "keywords": ["Anthelmintics", "Male", "Adult", "Adolescent", "RC955-962", "Helminthiasis", "Real-Time Polymerase Chain Reaction", "Albendazole", "Sensitivity and Specificity", "Feces", "Soil", "Treatment Outcome", "Arctic medicine. Tropical medicine", "Depsipeptides", "Helminths", "Humans", "Animals", "Female", "Public aspects of medicine", "RA1-1270", "Child", "Ascaris lumbricoides", "Parasite Egg Count", "Research Article", "Randomized Controlled Trials as Topic"], "contacts": [{"organization": "Christian N. Lotz, Emmanuel C. Mrimi, Pierre H. H. Schneeberger, Said M. Ali, Jan Hattendorf, Jennifer Keiser,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/PMC11835329"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20Neglected%20Tropical%20Diseases", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11835329", "name": "item", "description": "PMC11835329", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11835329"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-18T00:00:00Z"}}, {"id": "PMC2677447", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:24:40Z", "type": "Journal Article", "created": "2008-04-14", "title": "\u03b2\u2010catenin signalling in mesenchymal islet\u2010derived precursor cells", "description": "<p>Abstract.\uffe2\uff80\uff82  Objectives: Previously, we characterized human islet\uffe2\uff80\uff90derived precursor cells (hIPCs) as mesenchymal stem cells that migrate out from islets in vitro and can differentiate into functional islet\uffe2\uff80\uff90like structures following proliferative expansion. Here, we investigate the role of \uffce\uffb2\uffe2\uff80\uff90catenin signalling in derivation and proliferation of hIPCs. Materials and methods: Localization of \uffce\uffb2\uffe2\uff80\uff90catenin was performed using confocal microscopy. Expression levels of \uffce\uffb2\uffe2\uff80\uff90catenin target genes were measured by quantitative real\uffe2\uff80\uff90time polymerase chain reaction. Loss\uffe2\uff80\uff90of\uffe2\uff80\uff90function studies were performed using specific short interfering RNAs. Results: Immunostaining of islet outgrowths revealed translocation of \uffce\uffb2\uffe2\uff80\uff90catenin from plasma membranes in intact islets to the nucleus in cells migrating out. There were no nuclear \uffce\uffb2\uffe2\uff80\uff90catenin\uffe2\uff80\uff90positive cells in intact islets whereas between 35% and 70% of cells in established hIPC cultures exhibited nuclear \uffce\uffb2\uffe2\uff80\uff90catenin. Transcripts for \uffce\uffb2\uffe2\uff80\uff90catenin target genes were increased in hIPCs compared to those in islets. \uffce\uffb2\uffe2\uff80\uff90Catenin translocated to the cell membrane when hIPCs formed epithelial cell clusters. In proliferating hIPCs, there was a strong correlation between markers of proliferation and nuclear \uffce\uffb2\uffe2\uff80\uff90catenin. Treatment of hIPCs with the glycogen synthase kinase\uffe2\uff80\uff903\uffce\uffb2 inhibitor (2\uffe2\uff80\uffb2Z,3\uffe2\uff80\uffb2E)\uffe2\uff80\uff906\uffe2\uff80\uff90Bromoindirubin\uffe2\uff80\uff903\uffe2\uff80\uffb2\uffe2\uff80\uff90oxime increased intracellular \uffce\uffb2\uffe2\uff80\uff90catenin but reduced nuclear \uffce\uffb2\uffe2\uff80\uff90catenin, and was associated with reduced cell proliferation. Finally, knockdown of \uffce\uffb2\uffe2\uff80\uff90catenin decreased \uffce\uffb2\uffe2\uff80\uff90catenin target gene expression and hIPC proliferation. Conclusions: These results support a functional role for \uffce\uffb2\uffe2\uff80\uff90catenin during proliferation of hIPCs and suggest that activated \uffce\uffb2\uffe2\uff80\uff90catenin signalling may also be important during hIPC derivation from islets.</p", "keywords": ["Cell Nucleus", "0301 basic medicine", "0303 health sciences", "Transcription", " Genetic", "Stem Cells", "Epithelium", "Mesoderm", "Wnt Proteins", "Islets of Langerhans", "Protein Transport", "03 medical and health sciences", "Humans", "Intercellular Signaling Peptides and Proteins", "Biomarkers", "beta Catenin", "Cell Proliferation", "Signal Transduction"]}, "links": [{"href": "https://doi.org/PMC2677447"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Cell%20Proliferation", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC2677447", "name": "item", "description": "PMC2677447", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC2677447"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-04-14T00:00:00Z"}}, {"id": "PMC3500623", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:24:40Z", "type": "Journal Article", "created": "2012-07-19", "title": "Probing exchange kinetics and atomic resolution dynamics in high-molecular-weight complexes using dark-state exchange saturation transfer NMR spectroscopy", "description": "We present the protocol for the measurement and analysis of dark-state exchange saturation transfer (DEST), a novel solution NMR method for characterizing, at atomic resolution, the interaction between an NMR-'visible' free species and an NMR-'invisible' species transiently bound to a very high-molecular-weight (>1 MDa) macromolecular entity. The reduced rate of reorientational motion in the bound state that precludes characterization by traditional NMR methods permits the observation of DEST. (15)N-DEST profiles are measured on a sample comprising the dark state in exchange with an NMR-visible species; in addition, the difference (\u0394R(2)) in (15)N transverse relaxation rates between this sample and a control sample comprising only the NMR-visible species is also obtained. The (15)N-DEST and \u0394R(2) data for all residues are then fitted simultaneously to the McConnell equations for various exchange models describing the residue-specific dynamics in the bound state(s) and the interconversion rate constants. Although the length of the experiments depends strongly on sample conditions, approximately 1 week of NMR spectrometer time was sufficient for full characterization of samples of amyloid-\u03b2 (A\u03b2) at concentrations of ~100 \u03bcM.", "keywords": ["Molecular Weight", "0301 basic medicine", "Kinetics", "0303 health sciences", "03 medical and health sciences", "Amyloid beta-Peptides", "Time Factors", "Nitrogen Isotopes", "Macromolecular Substances", "Nuclear Magnetic Resonance", " Biomolecular", "Software"]}, "links": [{"href": "https://doi.org/PMC3500623"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Protocols", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC3500623", "name": "item", "description": "PMC3500623", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC3500623"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-07-19T00:00:00Z"}}, {"id": "PMC5986821", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:24:41Z", "type": "Journal Article", "created": "2018-05-29", "title": "Resolving molecule-specific information in dynamic lipid membrane processes with multi-resonant infrared metasurfaces", "description": "Abstract<p>A multitude of biological processes are enabled by complex interactions between lipid membranes and proteins. To understand such dynamic processes, it is crucial to differentiate the constituent biomolecular species and track their individual time evolution without invasive labels. Here, we present a label-free mid-infrared biosensor capable of distinguishing multiple analytes in heterogeneous biological samples with high sensitivity. Our technology leverages a multi-resonant metasurface to simultaneously enhance the different vibrational fingerprints of multiple biomolecules. By providing up to 1000-fold near-field intensity enhancement over both amide and methylene bands, our sensor resolves the interactions of lipid membranes with different polypeptides in real time. Significantly, we demonstrate that our label-free chemically specific sensor can analyze peptide-induced neurotransmitter cargo release from synaptic vesicle mimics. Our sensor opens up exciting possibilities for gaining new insights into biological processes such as signaling or transport in basic research as well as provides a valuable toolkit for bioanalytical and pharmaceutical applications.</p", "keywords": ["Science", "Circular Dichroism", "Q", "Lipid Bilayers", "Membrane Proteins", "Reproducibility of Results", "Biosensing Techniques", "02 engineering and technology", "01 natural sciences", "Article", "0104 chemical sciences", "Membrane Lipids", "Spectroscopy", " Fourier Transform Infrared", "Peptides", "0210 nano-technology", "Protein Binding"]}, "links": [{"href": "https://eprints.gla.ac.uk/164038/1/164038.pdf"}, {"href": "https://www.nature.com/articles/s41467-018-04594-x.pdf"}, {"href": "https://doi.org/PMC5986821"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC5986821", "name": "item", "description": "PMC5986821", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC5986821"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-06-04T00:00:00Z"}}, {"id": "PMC9574658", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:24:45Z", "type": "Journal Article", "created": "2021-10-26", "title": "Development of Inapparent Dengue Associated With Increased Antibody Levels to Aedes aegypti Salivary Proteins: A Longitudinal Dengue Cohort in Cambodia", "description": "Abstract                                   Background                   <p>We established the first prospective cohort to understand how infection with dengue virus is influenced by vector-specific determinants such as humoral immunity to Aedes aegypti salivary proteins.</p>                                                   Methods                   <p>Children aged 2\uffe2\uff80\uff939 years were enrolled in the PAGODAS (Pediatric Assessment Group of Dengue and Aedes Saliva) cohort with informed consent by their guardians. Children were followed semi-annually for antibodies to dengue and to proteins in Ae. aegypti salivary gland homogenate using enzyme-linked immunosorbent assays and dengue-specific neutralization titers. Children presented with fever at any time for dengue testing.</p>                                                   Results                   <p>From 13 July to 30 August 2018, we enrolled 771 children. At baseline, 22% (173/770) had evidence of neutralizing antibodies to 1 or more dengue serotypes. By April 2020, 51 children had symptomatic dengue while 148 dengue-naive children had inapparent dengue defined by neutralization assays. In a multivariate model, individuals with higher antibodies to Ae. aegypti salivary proteins were 1.5 times more likely to have dengue infection (hazard ratio [HR], 1.47 [95% confidence interval {CI}, 1.05\uffe2\uff80\uff932.06]; P\uffe2\uff80\uff85=\uffe2\uff80\uff85.02), particularly individuals with inapparent dengue (HR, 1.64 [95% CI, 1.12\uffe2\uff80\uff932.41]; P\uffe2\uff80\uff85=\uffe2\uff80\uff85.01).</p>                                                   Conclusions                   <p>High levels of seropositivity to Ae. aegypti salivary proteins are associated with future development of dengue infection, primarily inapparent, in dengue-naive Cambodian children.</p>                                                   Clinical Trials Registration                   <p>NCT03534245</p>", "keywords": ["0301 basic medicine", "Mosquito Vectors", "Medical and Health Sciences", "Microbiology", "Antibodies", "Vaccine Related", "Dengue", "03 medical and health sciences", "Rare Diseases", "Aedes aegypti", "Clinical Research", "Aedes", "Biodefense", "pediatric cohort", "Animals", "Humans", "mosquito saliva", "Prospective Studies", "Salivary Proteins and Peptides", "Child", "Neutralizing", "0303 health sciences", "Prevention", "Biological Sciences", "Dengue Virus", "dengue", "Antibodies", " Neutralizing", "3. Good health", "Vector-Borne Diseases", "Infectious Diseases", "Emerging Infectious Diseases", "Good Health and Well Being", "Infection", "Cambodia", "Biotechnology"]}, "links": [{"href": "https://doi.org/PMC9574658"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Journal%20of%20Infectious%20Diseases", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC9574658", "name": "item", "description": "PMC9574658", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC9574658"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-10-27T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Peptides&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Peptides&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Peptides&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Peptides&offset=24", "hreflang": "en-US"}], "numberMatched": 24, "numberReturned": 24, "distributedFeatures": [], "timeStamp": "2026-09-22T10:58:59.939224Z"}