{"type": "FeatureCollection", "features": [{"id": "10.1007/s00248-003-9001-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:41Z", "type": "Journal Article", "created": "2004-06-15", "title": "Microbial Community Structure And Oxidative Enzyme Activity In Nitrogen-Amended North Temperate Forest Soils", "description": "Large regions of temperate forest are subject to elevated atmospheric nitrogen (N) deposition which can affect soil organic matter dynamics by altering mass loss rates, soil respiration, and dissolved organic matter production. At present there is no general model that links these responses to changes in the organization and operation of microbial decomposer communities. Toward that end, we studied the response of litter and soil microbial communities to high levels of N amendment (30 and 80 kg ha(-1) yr(-1)) in three types of northern temperate forest: sugar maple/basswood (SMBW), sugar maple/red oak (SMRO), and white oak/black oak (WOBO). We measured the activity of extracellular enzymes (EEA) involved directly in the oxidation of lignin and humus (phenol oxidase, peroxidase), and indirectly, through the production of hydrogen peroxide (glucose oxidase, glyoxal oxidase). Community composition was analyzed by extracting and quantifying phospholipid fatty acids (PLFA) from soils. Litter EEA responses at SMBW sites diverged from those at oak-bearing sites (SMRO, BOWO), but the changes were not statistically significant. For soil, EEA responses were consistent across forests types: phenol oxidase and peroxidase activities declined as a function of N dose (33-73% and 5-41%, respectively, depending on forest type); glucose oxidase and glyoxal oxidase activities increased (200-400% and 150-300%, respectively, depending on forest type). Principal component analysis (PCA) ordinated forest types and treatment responses along two axes; factor 1 (44% of variance) was associated with phenol oxidase and peroxidase activities, factor 2 (31%) with glucose oxidase. Microbial biomass did not respond to N treatment, but nine of the 23 PLFA that formed >1 mol% of total biomass showed statistically significant treatment responses. PCA ordinated forest types and treatment responses along three axes (36%, 26%, 12% of variance). EEA factors 1 and 2 correlated negatively with PLFA factor 1 ( r = -0.20 and -0.35, respectively, n = 108) and positively with PLFA factor 3 ( r = +0.36 and +0.20, respectively, n = 108). In general, EEA responses were more strongly tied to changes in bacterial PLFA than to changes in fungal PLFA. Collectively, our data suggests that N inhibition of oxidative activity involves more than the repression of ligninase expression by white-rot basidiomycetes.", "keywords": ["Michigan", "Nitrogen", "Science", "Ecology and Evolutionary Biology", "Nature Conservation", "Microbiology", "Trees", "Soil", "Geoecology/Natural Processes", "Health Sciences", "Cellular and Developmental Biology", "Ecosystem", "Phospholipids", "Soil Microbiology", "2. Zero hunger", "Analysis of Variance", "Principal Component Analysis", "Ecology", "Life Sciences", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "15. Life on land", "Enzymes", "13. Climate action", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1007/s00248-003-9001-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-003-9001-x", "name": "item", "description": "10.1007/s00248-003-9001-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-003-9001-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-04-19T00:00:00Z"}}, {"id": "10.1007/s00248-007-9295-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:41Z", "type": "Journal Article", "created": "2007-08-31", "title": "Plfa Profiling Of Microbial Community Structure And Seasonal Shifts In Soils Of A Douglas-Fir Chronosequence", "description": "The impact and frequency of forest harvesting could significantly affect soil microbial community (SMC) structure and functioning. The ability of soil microorganisms to perform biogeochemical processes is critical for sustaining forest productivity and has a direct impact on decomposition dynamics and carbon storage potential. The Wind River Canopy Crane Research Forest in SW, WA, provided a unique opportunity to study a forest chronosequence and the residual effects of harvesting on the SMC in comparison to old-growth forests. The objective of this study was to determine the effect of clear-cutting and stand age on temporal dynamics of SMC and physiological stress markers using phospholipid fatty acid (PLFA) profiling. Soil microbial PLFA profiles were determined seven times over 22 months (Nov. 02 to Sep. 04) in old-growth coniferous forest stands (300-500 years) and 8 (CC8)- or 25 (CC25)-year-old replanted clear-cuts. PLFA patterns of the SMC shifted because of clear-cutting, but seasonal temporal changes had greater shifts than differences among stand age. The microbial biomass (total PLFA) and bacterial, fungal, and selected other PLFAs were significantly reduced in CC8 but not in CC25 sites relative to the old-growth sites. An increase in stress indicators [PLFA ratios of saturated/monsaturated and (cy17:0 + cy19:0)/(16:1omega7 + 18:1omega7)] in late summer was related to water stress. Although the canopy and litter input are quite different for a 25-year clear-cut compared to virgin old-growth forest, we conclude that the composition of the microbial communities, 25 years after clear-cutting, has recovered sufficiently to be much more similar to old-growth forests than a recent clear-cut at this Pacific Northwest forest site. The study shows the potential of PLFA analysis for profiling microbial communities and their stress status under field conditions, but wide temporal shifts emphasize the need for sampling over seasons to fully interpret ecosystem management impacts on microbial populations.", "keywords": ["Washington", "Time Factors", "Bacteria", "Fatty Acids", "Fungi", "Temperature", "Eukaryota", "Water", "04 agricultural and veterinary sciences", "15. Life on land", "Pseudotsuga", "Trees", "Soil", "13. Climate action", "Animals", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Seasons", "Phospholipids", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9295-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9295-1", "name": "item", "description": "10.1007/s00248-007-9295-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9295-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-31T00:00:00Z"}}, {"id": "10.1007/s00248-007-9308-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:41Z", "type": "Journal Article", "created": "2007-09-01", "title": "Long-Term Effect Of Municipal Solid Waste Amendment On Microbial Abundance And Humus-Associated Enzyme Activities Under Semiarid Conditions", "description": "Microbial ecology is the key to understanding the function of soil biota for organic matter cycling after a single amendment of organic waste in semiarid soils. Therefore, in this paper, the long-term effect (17 years) of adding different doses of a solid municipal waste to an arid soil on humus-enzyme complexes, a very stable and long-lasting fraction of soil enzymes, as well as on microbial and plant abundance, was studied. Humic substances were extracted by 0.1 M pH 7 sodium pyrophosphate from soil samples collected in experimental plots amended with different doses of a solid municipal waste (0, 65, 130, 195, and 260 t/ha) 17 years before. The activity of different hydrolases related with the C (beta-glucosidase), N (urease), and P (alkaline phosphatase) cycles and with the formation of humic substances (o-diphenol oxidase) were determined in this extract. The density and diversity of plant cover in the plots, as well as the fungal and bacterial biomass (by analyzing phopholipid fatty acids) were also determined. In general, the amended plots showed greater humic substance-related enzymatic activity than the unamended plots. This activity increased with the dose but only up to a certain level, above which it leveled off or even diminished. Plant diversity and cover density followed the same trend. Fungal and bacterial biomass also benefited in a dose-dependent manner. Different signature molecules representing gram+ and gram- bacteria, and those corresponding to monounsaturated and saturated fatty acids showed a similar behavior. The results demonstrate that organic amendment had a noticeable long-term effect on the vegetal development, humic substances-related enzyme activity and on the development of bacteria and fungi in semiarid conditions.", "keywords": ["2. Zero hunger", "Analysis of Variance", "Time Factors", "Bacteria", "Fatty Acids", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "6. Clean water", "Enzymes", "Refuse Disposal", "12. Responsible consumption", "Soil", "Ergosterol", "11. Sustainability", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Humic Substances", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9308-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9308-0", "name": "item", "description": "10.1007/s00248-007-9308-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9308-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-09-02T00:00:00Z"}}, {"id": "10.1007/s00248-010-9727-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:41Z", "type": "Journal Article", "created": "2010-08-03", "title": "Soil Microbial Abundance And Diversity Along A Low Precipitation Gradient", "description": "The exploration of spatial patterns of abundance and diversity patterns along precipitation gradients has focused for centuries on plants and animals; microbial profiles along such gradients are largely unknown. We studied the effects of soil pH, nutrient concentration, salinity, and water content on bacterial abundance and diversity in soils collected from Mediterranean, semi-arid, and arid sites receiving approximately 400, 300, and 100 mm annual precipitation, respectively. Bacterial diversity was evaluated by terminal restriction fragment length polymorphism and clone library analyses and the patterns obtained varied with the climatic regions. Over 75% of the sequenced clones were unique to their environment, while \u223c2% were shared by all sites, yet, the Mediterranean and semi-arid sites had more common clones (\u223c9%) than either had with the arid site (4.7% and 6%, respectively). The microbial abundance, estimated by phospholipid fatty acids and real-time quantitative PCR assays, was significantly lower in the arid region. Our results indicate that although soil bacterial abundance decreases with precipitation, bacterial diversity is independent of precipitation gradient. Furthermore, community composition was found to be unique to each ecosystem.", "keywords": ["DNA", " Bacterial", "2. Zero hunger", "0301 basic medicine", "Salinity", "0303 health sciences", "Bacteria", "Mediterranean Region", "Climate", "Rain", "Water", "Biodiversity", "Hydrogen-Ion Concentration", "15. Life on land", "Soil", "03 medical and health sciences", "Phospholipids", "Polymorphism", " Restriction Fragment Length", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-010-9727-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-010-9727-1", "name": "item", "description": "10.1007/s00248-010-9727-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-010-9727-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-01T00:00:00Z"}}, {"id": "10.1016/j.bmcl.2024.129690", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:15:56Z", "type": "Journal Article", "created": "2024-03-05", "title": "Identification of two novel chemical classes of Autotaxin (ATX) inhibitors using Enalos Asclepios KNIME nodes", "description": "Autotaxin is a secreted lysophospholipase D which is a member of the ectonucleotide pyrophosphatase/phosphodiesterase family converting extracellular lysophosphatidylcholine and other non-choline lysophospholipids, such as lysophosphatidylethanolamine and lysophosphatidylserine, to the lipid mediator lysophosphatidic acid. Autotaxin is implicated in various fibroproliferative diseases including interstitial lung diseases, such as idiopathic pulmonary fibrosis and hepatic fibrosis, as well as in cancer. In this study, we present an effort of identifying ATX inhibitors that bind to allosteric ATX binding sites using the Enalos Asclepios KNIME Node. All the available PDB crystal structures of ATX were collected, prepared, and aligned. Visual examination of these structures led to the identification of four crystal structures of human ATX co-crystallized with four known inhibitors. These inhibitors bind to five binding sites with five different binding modes. These five binding sites were thereafter used to virtually screen a compound library of 14,000 compounds to identify molecules that bind to allosteric sites. Based on the binding mode and interactions, the docking score, and the frequency that a compound comes up as a top-ranked among the five binding sites, 24 compounds were selected for in vitro testing. Finally, two compounds emerged with inhibitory activity against ATX in the low micromolar range, while their mode of inhibition and binding pattern were also studied. The two derivatives identified herein can serve as 'hits' towards developing novel classes of ATX allosteric inhibitors.", "keywords": ["Binding Sites", "Phosphoric Diester Hydrolases", "Neoplasms", "Humans", "Lysophospholipids", "Allosteric Site"]}, "links": [{"href": "https://doi.org/10.1016/j.bmcl.2024.129690"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioorganic%20%26amp%3B%20Medicinal%20Chemistry%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.bmcl.2024.129690", "name": "item", "description": "10.1016/j.bmcl.2024.129690", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.bmcl.2024.129690"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2013.03.090", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:53Z", "type": "Journal Article", "created": "2013-04-24", "title": "Microbial Utilisation Of Biochar-Derived Carbon", "description": "Whilst largely considered an inert material, biochar has been documented to contain a small yet significant fraction of microbially available labile organic carbon (C). Biochar addition to soil has also been reported to alter soil microbial community structure, and to both stimulate and retard the decomposition of native soil organic matter (SOM). We conducted a short-term incubation experiment using two (13)C-labelled biochars produced from wheat or eucalypt shoots, which were incorporated in an aridic arenosol to examine the fate of the labile fraction of biochar-C through the microbial community. This was achieved using compound specific isotopic analysis (CSIA) of phospholipid fatty acids (PLFAs). A proportion of the biologically-available fraction of both biochars was rapidly (within three days) utilised by gram positive bacteria. There was a sharp peak in CO2 evolution shortly after biochar addition, resulting from rapid turnover of labile C components in biochars and through positive priming of native SOM. Our results demonstrate that this CO2 evolution was at least partially microbially mediated, and that biochar application to soil can cause significant and rapid changes in the soil microbial community; likely due to addition of labile C and increases in soil pH.", "keywords": ["Carbon sequestration", "[SDE] Environmental Sciences", "Carbon Sequestration", "Chromatography", " Gas", "Magnetic Resonance Spectroscopy", "550", "short term", "[SDV]Life Sciences [q-bio]", "growth", "black carbon", "Char", "01 natural sciences", "630", "Mass Spectrometry", "c 13 plfa", "Black carbon", "soil organic matter", "Soil Pollutants", "mineralization", "Organic carbon", "Phospholipids", "Soil Microbiology", "char", "0105 earth and related environmental sciences", "2. Zero hunger", "Carbon Isotopes", "decomposition", "wheat straw", "biomass", "organic carbon", "Fatty Acids", "Western Australia", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "540", "pyrolysis", "forest soil", "carbon sequestration", "Carbon", "[SDV] Life Sciences [q-bio]", "Charcoal", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries", "community structure", "\u00b9\u00b3C-PLFA", "Pyrolysis"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2013.03.090"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2013.03.090", "name": "item", "description": "10.1016/j.scitotenv.2013.03.090", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2013.03.090"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-11-01T00:00:00Z"}}, {"id": "10.1038/s41396-023-01527-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:47Z", "type": "Journal Article", "created": "2023-10-18", "title": "Community composition and physiological plasticity control microbial carbon storage across natural and experimental soil fertility gradients", "description": "Abstract                <p>Many microorganisms synthesise carbon (C)-rich compounds under resource deprivation. Such compounds likely serve as intracellular C-storage pools that sustain the activities of microorganisms growing on stoichiometrically imbalanced substrates, making them potentially vital to the function of ecosystems on infertile soils. We examined the dynamics and drivers of three putative C-storage compounds (neutral lipid fatty acids [NLFAs], polyhydroxybutyrate [PHB], and trehalose) across a natural gradient of soil fertility in eastern Australia. Together, NLFAs, PHB, and trehalose corresponded to 8.5\uffe2\uff80\uff9340% of microbial C and 0.06\uffe2\uff80\uff930.6% of soil organic C. When scaled to \uffe2\uff80\uff9cstructural\uffe2\uff80\uff9d microbial biomass (indexed by polar lipid fatty acids; PLFAs), NLFA and PHB allocation was 2\uffe2\uff80\uff933-times greater in infertile soils derived from ironstone and sandstone than in comparatively fertile basalt- and shale-derived soils. PHB allocation was positively correlated with belowground biological phosphorus (P)-demand, while NLFA allocation was positively correlated with fungal PLFA : bacterial PLFA ratios. A complementary incubation revealed positive responses of respiration, storage, and fungal PLFAs to glucose, while bacterial PLFAs responded positively to PO43-. By comparing these results to a model of microbial C-allocation, we reason that NLFA primarily served the \uffe2\uff80\uff9creserve\uffe2\uff80\uff9d storage mode for C-limited taxa (i.e., fungi), while the variable portion of PHB likely served as \uffe2\uff80\uff9csurplus\uffe2\uff80\uff9d C-storage for P-limited bacteria. Thus, our findings reveal a convergence of community-level processes (i.e., changes in taxonomic composition that underpin reserve-mode storage dynamics) and intracellular mechanisms (e.g., physiological plasticity of surplus-mode storage) that drives strong, predictable community-level microbial C-storage dynamics across gradients of soil fertility and substrate stoichiometry.</p", "keywords": ["2. Zero hunger", "Science & Technology", "Ecology", "Fatty Acids", "Fungi", "Soil Science", "Trehalose", "Environmental Sciences & Ecology", "15. Life on land", "Markvetenskap", "Microbiology", "Article", "Carbon", "Environmental sciences", "Biological sciences", "Soil", "Biomass", "Life Sciences & Biomedicine", "Ecosystem", "Soil Microbiology", "Phospholipids"]}, "links": [{"href": "https://doi.org/10.1038/s41396-023-01527-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41396-023-01527-5", "name": "item", "description": "10.1038/s41396-023-01527-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41396-023-01527-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-18T00:00:00Z"}}, {"id": "10.1100/tsw.2001.450", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:32Z", "type": "Journal Article", "created": "2005-03-23", "title": "The Impact Of Long-Term Nitrogen Addition On Microbial Community Composition In Three Hawaiian Forest Soils", "description": "<p>We evaluated the microbial communities in three Hawaiian forest soils along a natural fertility gradient and compared their distinct responses to long-term nitrogen (N) additions. The sites studied have the same elevation, climate, and dominant vegetation, but vary in age of development, and thus in soil nutrient availability and nutrient limitation to plant growth. Fertilized plots at each site have received 100 kg ha year-1N addition for at least 8 years. Soil parameters, water content, pH, and ammonium and nitrate availability differed by site, but not between control and N-addition treatments within a site at the time of sampling. Microbial biomass also varied by site, but was not affected by N addition. In contrast, microbial community composition (measured by phospholipid analysis) varied among sites and between control and N-addition plots within a site. These data suggest that microbial community composition responds to N addition even when plant net primary productivity is limited by nutrients other than N. This may have implications for the behavior of forests impacted by atmospheric N deposition that are considered to be \uffe2\uff80\uff9cnitrogen saturated,\uffe2\uff80\uff9d yet still retain N in the soil.</p>", "keywords": ["2. Zero hunger", "Technology", "Tropical Climate", "Bacteria", "Nitrogen", "T", "Science", "Q", "R", "Fungi", "Water", "04 agricultural and veterinary sciences", "15. Life on land", "Hawaii", "6. Clean water", "Time", "Trees", "Soil", "Viruses", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Phospholipids", "Soil Microbiology", "Research Article"], "contacts": [{"organization": "Teri C. Balser", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1100/tsw.2001.450"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Scientific%20World%20JOURNAL", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1100/tsw.2001.450", "name": "item", "description": "10.1100/tsw.2001.450", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1100/tsw.2001.450"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2001-01-01T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2010.01032.x", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:19:02Z", "type": "Journal Article", "created": "2011-01-11", "title": "Fungal And Bacterial Growth Responses To N Fertilization And Ph In The 150-Year 'Park Grass' Uk Grassland Experiment", "description": "The effects of nitrogen (N) fertilization (0-150 kg N ha\u207b\u00b9 year\u207b\u00b9 since 1865) and pH (3.3-7.4) on fungal and bacterial growth, biomass and phospholipid fatty acid (PLFA) composition were investigated in grassland soils from the 'Park Grass Experiment', Rothamsted Research, UK. Bacterial growth decreased and fungal growth increased with lower pH, resulting in a 50-fold increase in the relative importance of fungi between pH 7.4 and 3.3. The PLFA-based fungal:bacterial biomass ratio was unchanged between pH 4.5 and 7.4, and decreased only below pH 4.5. Respiration and substrate-induced respiration biomass both decreased three- to fourfold with lower pH, but biomass concentrations estimated using PLFAs were unaffected by pH. N fertilization did not affect bacterial growth and marginally affected fungal growth while PLFA biomass marker concentrations were all reduced by higher N additions. Respiration decreased with higher N application, suggesting a reduced quality of the soil organic carbon. The PLFA composition was strongly affected by both pH and N. A comparison with a pH gradient in arable soil allowed us to generalize the pH effect between systems. There are 30-50-fold increases in the relative importance of fungi between high (7.4-8.3) and low (3.3-4.5) pH with concomitant reductions of respiration by 30-70%.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Nitrogen", "Fatty Acids", "Fungi", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Hydrogen-Ion Concentration", "15. Life on land", "Poaceae", "Microbiology", "United Kingdom", "03 medical and health sciences", "Soil", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Phospholipids", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2010.01032.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2010.01032.x", "name": "item", "description": "10.1111/j.1574-6941.2010.01032.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2010.01032.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-01-17T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2010.03321.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:59Z", "type": "Journal Article", "created": "2010-06-11", "title": "Belowground Carbon Allocation By Trees Drives Seasonal Patterns Of Extracellular Enzyme Activities By Altering Microbial Community Composition In A Beech Forest Soil", "description": "*Plant seasonal cycles alter carbon (C) and nitrogen (N) availability for soil microbes, which may affect microbial community composition and thus feed back on microbial decomposition of soil organic material and plant N availability. The temporal dynamics of these plant-soil interactions are, however, unclear. *Here, we experimentally manipulated the C and N availability in a beech forest through N fertilization or tree girdling and conducted a detailed analysis of the seasonal pattern of microbial community composition and decomposition processes over 2 yr. *We found a strong relationship between microbial community composition and enzyme activities over the seasonal course. Phenoloxidase and peroxidase activities were highest during late summer, whereas cellulase and protease peaked in late autumn. Girdling, and thus loss of mycorrhiza, resulted in an increase in soil organic matter-degrading enzymes and a decrease in cellulase and protease activity. *Temporal changes in enzyme activities suggest a switch of the main substrate for decomposition between summer (soil organic matter) and autumn (plant litter). Our results indicate that ectomycorrhizal fungi are possibly involved in autumn cellulase and protease activity. Our study shows that, through belowground C allocation, trees significantly alter soil microbial communities, which may affect seasonal patterns of decomposition processes.", "keywords": ["Nitrogen", "Climate", "Trees", "Soil", "Mycorrhizae", "401902 Soil science", "Fagus", "Biomass", "Phospholipids", "Soil Microbiology", "106022 Mikrobiologie", "Bacteria", "Research", "Temperature", "04 agricultural and veterinary sciences", "15. Life on land", "401902 Bodenkunde", "Carbon", "Solubility", "106030 Pflanzen\u00f6kologie", "106022 Microbiology", "Regression Analysis", "0401 agriculture", " forestry", " and fisheries", "106030 Plant ecology", "Seasons", "Extracellular Space", "Biomarkers"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2010.03321.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2010.03321.x", "name": "item", "description": "10.1111/j.1469-8137.2010.03321.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2010.03321.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-07-19T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2007.00318.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:02Z", "type": "Journal Article", "created": "2007-04-18", "title": "Response Of Soil Microbial Biomass And Community Structures To Conventional And Organic Farming Systems Under Identical Crop Rotations", "description": "In this study the influence of different farming systems on microbial community structure was analyzed using soil samples from the DOK long-term field experiment in Switzerland, which comprises organic (BIODYN and BIOORG) and conventional (CONFYM and CONMIN) farming systems as well as an unfertilized control (NOFERT). We examined microbial communities in winter wheat plots at two different points in the crop rotation (after potatoes and after maize). Employing extended polar lipid analysis up to 244 different phospholipid fatty acids (PLFA) and phospholipid ether lipids (PLEL) were detected. Higher concentrations of PLFA and PLEL in BIODYN and BIOORG indicated a significant influence of organic agriculture on microbial biomass. Farmyard manure (FYM) application consistently revealed the strongest, and the preceding crop the weakest, influence on domain-specific biomass, diversity indices and microbial community structures. Esterlinked PLFA from slowly growing bacteria (k-strategists) showed the strongest responses to long-term organic fertilization. Although the highest fungal biomass was found in the two organic systems of the DOK field trial, their contribution to the differentiation of community structures according to the management regime was relatively low. Prokaryotic communities responded most strongly to either conventional or organic farming management.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Nutrient turnover", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Zea mays", "Soil quality", "Soil", "organic farming; DOK long-term field trial; microbial community; PLFA; PLEL", "0401 agriculture", " forestry", " and fisheries", "'Organics' in general", "Fertilizers", "Ecosystem", "Phospholipids", "Soil Microbiology", "Triticum", "Solanum tuberosum"], "contacts": [{"organization": "Espersch\u00fctz, J\u00fcrgen, Gattinger, Andreas, M\u00e4der, Paul, Schloter, Michael, Flie\u00dfbach, Andreas,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2007.00318.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2007.00318.x", "name": "item", "description": "10.1111/j.1574-6941.2007.00318.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2007.00318.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-07-01T00:00:00Z"}}, {"id": "10.1111/nph.14083", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:05Z", "type": "Journal Article", "created": "2016-07-11", "title": "Increased Phosphate Uptake But Not Resorption Alleviates Phosphorus Deficiency Induced By Nitrogen Deposition In Temperate Larix Principis-Rupprechtii Plantations", "description": "Summary<p>   <p>The imbalance between nitrogen (N) and phosphorus (P) deposition may shift temperate ecosystems from N\uffe2\uff80\uff90 to P\uffe2\uff80\uff90limitation. However, it is unclear how the imbalanced N\uffc2\uffa0:\uffc2\uffa0P input affects the strategies of plants to acquire P and, therefore, the growth of plants and the competition among species.</p>  <p>We conducted a 4\uffe2\uff80\uff90yr N\uffe2\uff80\uff90addition experiment in young and mature larch (Larix principis\uffe2\uff80\uff90rupprechtii) stands. Plant growth and P acquisition strategies were assessed for larch and understorey vegetation.</p>  <p>N addition stimulated the aboveground productivity of understorey vegetation in the young stand and larch in the mature stand, with other species unaffected. The competitive advantages of understorey vegetation in the young stand and larch in the mature stand were associated with their high stoichiometric homoeostasis. To maintain the N\uffc2\uffa0:\uffc2\uffa0P homoeostasis of these species, an increase in phosphatase activity but not P resorption efficiency increased the supply of P. Additionally, N addition accelerated P mineralization by decreasing the fungal\uffe2\uff80\uff90to\uffe2\uff80\uff90bacterial ratios and improved uptake of soil P by increasing the arbuscular mycorrhizas\uffe2\uff80\uff90to\uffe2\uff80\uff90ectomycorrhizas ratios.</p>  <p>Our results suggest that plants with high stoichiometric homoeostasis could better cope with N deposition\uffe2\uff80\uff90induced P\uffe2\uff80\uff90deficiency. Although P resorption efficiency showed little plasticity in response, plants activated a variety of P\uffe2\uff80\uff90acquisition pathways to alleviate the P\uffe2\uff80\uff90deficiency caused by N deposition.</p>  </p>", "keywords": ["2. Zero hunger", "Analysis of Variance", "Bacteria", "Nitrogen", "Acid Phosphatase", "Fatty Acids", "Fungi", "Larix", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "Phosphates", "Plant Leaves", "Species Specificity", "Mycorrhizae", "Homeostasis", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Phospholipids"]}, "links": [{"href": "https://doi.org/10.1111/nph.14083"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.14083", "name": "item", "description": "10.1111/nph.14083", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.14083"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-07-11T00:00:00Z"}}, {"id": "10.1371%2fjournal.pone.0060441", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:29Z", "type": "Journal Article", "created": "2013-03-27", "title": "Nitrogen Addition And Warming Independently Influence The Belowground Micro-Food Web In A Temperate Steppe", "description": "Climate warming and atmospheric nitrogen (N) deposition are known to influence ecosystem structure and functioning. However, our understanding of the interactive effect of these global changes on ecosystem functioning is relatively limited, especially when it concerns the responses of soils and soil organisms. We conducted a field experiment to study the interactive effects of warming and N addition on soil food web. The experiment was established in 2006 in a temperate steppe in northern China. After three to four years (2009-2010), we found that N addition positively affected microbial biomass and negatively influenced trophic group and ecological indices of soil nematodes. However, the warming effects were less obvious, only fungal PLFA showed a decreasing trend under warming. Interestingly, the influence of N addition did not depend on warming. Structural equation modeling analysis suggested that the direct pathway between N addition and soil food web components were more important than the indirect connections through alterations in soil abiotic characters or plant growth. Nitrogen enrichment also affected the soil nematode community indirectly through changes in soil pH and PLFA. We conclude that experimental warming influenced soil food web components of the temperate steppe less than N addition, and there was little influence of warming on N addition effects under these experimental conditions.", "keywords": ["China", "Food Chain", "Nematoda", "Nitrogen", "Science", "deposition", "Models", " Biological", "northern china", "Soil", "soil nematodes", "Animals", "Biomass", "organic-matter", "global change", "Phospholipids", "Soil Microbiology", "2. Zero hunger", "elevated co2", "Analysis of Variance", "species composition", "Q", "R", "Temperature", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "13. Climate action", "international", "climate-change manipulations", "plant-communities", "Medicine", "0401 agriculture", " forestry", " and fisheries", "community structure", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371%2fjournal.pone.0060441"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371%2fjournal.pone.0060441", "name": "item", "description": "10.1371%2fjournal.pone.0060441", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371%2fjournal.pone.0060441"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-03-27T00:00:00Z"}}, {"id": "10.13227/j.hjkx.2016.09.042", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:28Z", "type": "Journal Article", "description": "Nitrogen deposition increases the productivity of forest-land, yet may cause environmental damage, while soil microorganisms are sensitive to the ecological environment. The objective of this study was to investigate the effects of nitrogen deposition on soil microbial communities and the relationship among soil microbial communities, soil temperature, soil moisture and the concentration of nitrogen deposition in the Masson pine forest. The results might provide a reference for further study on the effects of nitrogen deposition on forest ecosystem of Masson pine. From May 2014 to June 2015, three nitrogen application treatments and the control treatment were set up:low nitrogen [N20,20 g\u00b7(m2\u00b7a)-1], moderate nitrogen [N40, 40 g\u00b7(m2\u00b7a)-1], high nitrogen [N60, 60 g\u00b7(m2\u00b7a)-1] and control treatment [N0, 0 g\u00b7(m2\u00b7a)-1] in the Masson pine forest. Soil microbial community structure, soil temperature and soil moisture were measured by phosphorus lipid fatty acids (PLFAs) and automated soil CO2 exchange station. The results showed that:1 Seasonal variation had a remarkable effect on soil bacteria, fungi, actinomycetes and the total PLFA(P<0.05), all kinds of soil microorganisms had the highest amounts in spring and the lowest in winter. In different seasons, the response of different soil microorganisms to nitrogen deposition was different. Generally, nitrogen deposition decreased soil microorganisms in spring and autumn, while in summer and winter, nitrogen deposition had a positive effect on soil microorganisms. 2 Nitrogen deposition had a significant effect on soil microbial community structure(P<0.05). In spring and summer, low nitrogen and moderate nitrogen decreased the soil microbial richness index and diversity index, yet increased the evenness index. In the autumn and winter, low nitrogen and moderate nitrogen promoted the soil microbial richness index, diversity index and evenness index. In all four seasons, nitrogen deposition reduced the soil microbial richness index, diversity index and evenness index. 3 Correlation analysis showed that the concentration of nitrogen deposition had a very significant negative correlation with soil bacteria (P<0.01), and a significant negative correlation with total PLFA (P<0.05). Soil temperature had a significant negative correlation with soil actinomycetes. Soil moisture had a significant positive correlation with bacteria and total PLFA. In conclusion, soil microorganisms of Masson pine forest were mainly influenced by soil moisture and less affected by soil temperature in Jinyun Mountain. Nitrogen deposition had a significant effect on microbial community structure and diversity.", "keywords": ["China", "Nitrogen", "Fatty Acids", "0211 other engineering and technologies", "Fungi", "02 engineering and technology", "Forests", "Pinus", "01 natural sciences", "Soil", "Ecosystem", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Bing-Hui He, Qing-Ping Zeng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.13227/j.hjkx.2016.09.042"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Huan%20jing%20ke%20xue%3D%20Huanjing%20kexue", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.13227/j.hjkx.2016.09.042", "name": "item", "description": "10.13227/j.hjkx.2016.09.042", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.13227/j.hjkx.2016.09.042"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0056532", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:31Z", "type": "Journal Article", "created": "2013-02-20", "title": "Fungi Benefit From Two Decades Of Increased Nutrient Availability In Tundra Heath Soil", "description": "If microbial degradation of carbon substrates in arctic soil is stimulated by climatic warming, this would be a significant positive feedback on global change. With data from a climate change experiment in Northern Sweden we show that warming and enhanced soil nutrient availability, which is a predicted long-term consequence of climatic warming and mimicked by fertilization, both increase soil microbial biomass. However, while fertilization increased the relative abundance of fungi, warming caused only a minimal shift in the microbial community composition based on the phospholipid fatty acid (PLFA) and neutral lipid fatty acid (NLFA) profiles. The function of the microbial community was also differently affected, as indicated by stable isotope probing of PLFA and NLFA. We demonstrate that two decades of fertilization have favored fungi relative to bacteria, and increased the turnover of complex organic compounds such as vanillin, while warming has had no such effects. Furthermore, the NLFA-to-PLFA ratio for (13)C-incorporation from acetate increased in warmed plots but not in fertilized ones. Thus, fertilization cannot be used as a proxy for effects on warming in arctic tundra soils. Furthermore, the different functional responses suggest that the biomass increase found in both fertilized and warmed plots was mediated via different mechanisms.", "keywords": ["Sweden", "2. Zero hunger", "Arctic Regions", "Science", "Climate Change", "Q", "R", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "Soil", "13. Climate action", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Ecosystem", "Phospholipids", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0056532"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0056532", "name": "item", "description": "10.1371/journal.pone.0056532", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0056532"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-02-20T00:00:00Z"}}, {"id": "10.1371/journal.pone.0060441", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:31Z", "type": "Journal Article", "created": "2013-03-27", "title": "Nitrogen Addition And Warming Independently Influence The Belowground Micro-Food Web In A Temperate Steppe", "description": "Climate warming and atmospheric nitrogen (N) deposition are known to influence ecosystem structure and functioning. However, our understanding of the interactive effect of these global changes on ecosystem functioning is relatively limited, especially when it concerns the responses of soils and soil organisms. We conducted a field experiment to study the interactive effects of warming and N addition on soil food web. The experiment was established in 2006 in a temperate steppe in northern China. After three to four years (2009-2010), we found that N addition positively affected microbial biomass and negatively influenced trophic group and ecological indices of soil nematodes. However, the warming effects were less obvious, only fungal PLFA showed a decreasing trend under warming. Interestingly, the influence of N addition did not depend on warming. Structural equation modeling analysis suggested that the direct pathway between N addition and soil food web components were more important than the indirect connections through alterations in soil abiotic characters or plant growth. Nitrogen enrichment also affected the soil nematode community indirectly through changes in soil pH and PLFA. We conclude that experimental warming influenced soil food web components of the temperate steppe less than N addition, and there was little influence of warming on N addition effects under these experimental conditions.", "keywords": ["China", "Food Chain", "Nematoda", "Nitrogen", "Science", "deposition", "Models", " Biological", "northern china", "Soil", "soil nematodes", "Animals", "Biomass", "organic-matter", "global change", "Phospholipids", "Soil Microbiology", "2. Zero hunger", "elevated co2", "Analysis of Variance", "species composition", "Q", "R", "Temperature", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "13. Climate action", "international", "climate-change manipulations", "plant-communities", "Medicine", "0401 agriculture", " forestry", " and fisheries", "community structure", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0060441"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0060441", "name": "item", "description": "10.1371/journal.pone.0060441", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0060441"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-03-27T00:00:00Z"}}, {"id": "10.1371/journal.pone.0061188", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:31Z", "type": "Journal Article", "created": "2013-04-12", "title": "Interactive Effects Of Nitrogen And Phosphorus On Soil Microbial Communities In A Tropical Forest", "description": "Elevated nitrogen (N) deposition in humid tropical regions may exacerbate phosphorus (P) deficiency in forests on highly weathered soils. However, it is not clear how P availability affects soil microbes and soil carbon (C), or how P processes interact with N deposition in tropical forests. We examined the effects of N and P additions on soil microbes and soil C pools in a N-saturated old-growth tropical forest in southern China to test the hypotheses that (1) N and P addition will have opposing effects on soil microbial biomass and activity, (2) N and P addition will alter the composition of the microbial community, (3) the addition of N and P will have interactive effects on soil microbes and (4) addition-mediated changes in microbial communities would feed back on soil C pools. Phospholipid fatty acid (PLFA) analysis was used to quantify the soil microbial community following four treatments: Control, N addition (15 g N m(-2) yr(-1)), P addition (15 g P m(-2) yr(-1)), and N&P addition (15 g N m(-2) yr(-1) plus 15 g P m(-2) yr(-1)). These were applied from 2007 to 2011. Whereas additions of P increased soil microbial biomass, additions of N reduced soil microbial biomass. These effects, however, were transient, disappearing over longer periods. Moreover, N additions significantly increased relative abundance of fungal PLFAs and P additions significantly increased relative abundance of arbuscular mycorrhizal (AM) fungi PLFAs. Nitrogen addition had a negative effect on light fraction C, but no effect on heavy fraction C and total soil C. In contrast, P addition significantly decreased both light fraction C and total soil C. However, there were no interactions between N addition and P addition on soil microbes. Our results suggest that these nutrients are not co-limiting, and that P rather than N is limiting in this tropical forest.", "keywords": ["2. Zero hunger", "China", "Tropical Climate", "Bacteria", "Nitrogen", "Science", "Q", "Fatty Acids", "R", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Trees", "Soil", "13. Climate action", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Phospholipids", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0061188"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0061188", "name": "item", "description": "10.1371/journal.pone.0061188", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0061188"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-12T00:00:00Z"}}, {"id": "10.1371/journal.pone.0092985", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:32Z", "type": "Journal Article", "created": "2014-03-25", "title": "Comparison Of Seasonal Soil Microbial Process In Snow-Covered Temperate Ecosystems Of Northern China", "description": "Open AccessMore than half of the earth's terrestrial surface currently experiences seasonal snow cover and soil frost. Winter compositional and functional investigations in soil microbial community are frequently conducted in alpine tundra and boreal forest ecosystems. However, little information on winter microbial biogeochemistry is known from seasonally snow-covered temperate ecosystems. As decomposer microbes may differ in their ability/strategy to efficiently use soil organic carbon (SOC) within different phases of the year, understanding seasonal microbial process will increase our knowledge of biogeochemical cycling from the aspect of decomposition rates and corresponding nutrient dynamics. In this study, we measured soil microbial biomass, community composition and potential SOC mineralization rates in winter and summer, from six temperate ecosystems in northern China. Our results showed a clear pattern of increased microbial biomass C to nitrogen (N) ratio in most winter soils. Concurrently, a shift in soil microbial community composition occurred with higher fungal to bacterial biomass ratio and gram negative (G-) to gram positive (G+) bacterial biomass ratio in winter than in summer. Furthermore, potential SOC mineralization rate was higher in winter than in summer. Our study demonstrated a distinct transition of microbial community structure and function from winter to summer in temperate snow-covered ecosystems. Microbial N immobilization in winter may not be the major contributor for plant growth in the following spring.", "keywords": ["Biomass (ecology)", "Atmospheric Science", "Microbial population biology", "Decomposer", "Nutrient cycle", "Physical Phenomena", "Agricultural and Biological Sciences", "Soil", "Terrestrial ecosystem", "Snow", "Soil water", "Biomass", "Phospholipids", "Soil Microbiology", "Minerals", "Glucan 1", "4-beta-Glucosidase", "Ecology", "Geography", "Mineralization (soil science)", "Q", "R", "Life Sciences", "04 agricultural and veterinary sciences", "Biogeochemistry", "16. Peace & justice", "Earth and Planetary Sciences", "Physical Sciences", "Medicine", "Seasons", "Ecosystem Functioning", "Research Article", "China", "Nitrogen", "Science", "Soil Science", "Biogeochemical cycle", "Environmental science", "Meteorology", "Genetics", "Arctic Permafrost Dynamics and Climate Change", "Tundra", "Biology", "Ecosystem", "Soil science", "Bacteria", "Fungi", "Microbial Diversity in Antarctic Ecosystems", "15. Life on land", "Carbon", "Temperate climate", "13. Climate action", "FOS: Biological sciences", "Environmental Science", "0401 agriculture", " forestry", " and fisheries", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems"], "contacts": [{"organization": "Xinyue Zhang, Wei Wang, Weile Chen, Naili Zhang, Hui Zeng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0092985"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0092985", "name": "item", "description": "10.1371/journal.pone.0092985", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0092985"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-03-25T00:00:00Z"}}, {"id": "10.1371/journal.pone.0096182", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:32Z", "type": "Journal Article", "created": "2014-05-09", "title": "Biotic And Abiotic Properties Mediating Plant Diversity Effects On Soil Microbial Communities In An Experimental Grassland", "description": "Plant diversity drives changes in the soil microbial community which may result in alterations in ecosystem functions. However, the governing factors between the composition of soil microbial communities and plant diversity are not well understood. We investigated the impact of plant diversity (plant species richness and functional group richness) and plant functional group identity on soil microbial biomass and soil microbial community structure in experimental grassland ecosystems. Total microbial biomass and community structure were determined by phospholipid fatty acid (PLFA) analysis. The diversity gradient covered 1, 2, 4, 8, 16 and 60 plant species and 1, 2, 3 and 4 plant functional groups (grasses, legumes, small herbs and tall herbs). In May 2007, soil samples were taken from experimental plots and from nearby fields and meadows. Beside soil texture, plant species richness was the main driver of soil microbial biomass. Structural equation modeling revealed that the positive plant diversity effect was mainly mediated by higher leaf area index resulting in higher soil moisture in the top soil layer. The fungal-to-bacterial biomass ratio was positively affected by plant functional group richness and negatively by the presence of legumes. Bacteria were more closely related to abiotic differences caused by plant diversity, while fungi were more affected by plant-derived organic matter inputs. We found diverse plant communities promoted faster transition of soil microbial communities typical for arable land towards grassland communities. Although some mechanisms underlying the plant diversity effect on soil microorganisms could be identified, future studies have to determine plant traits shaping soil microbial community structure. We suspect differences in root traits among different plant communities, such as root turnover rates and chemical composition of root exudates, to structure soil microbial communities.", "keywords": ["0301 basic medicine", "570", "Chromatography", " Gas", "Geography & travel", "Science", "910", "Plant Roots", "Gas Chromatography-Mass Spectrometry", "Soil", "03 medical and health sciences", "info:eu-repo/classification/ddc/910", "Biomass", "Phospholipids", "Soil Microbiology", "2. Zero hunger", "0303 health sciences", "Bacteria", "Q", "Fatty Acids", "R", "Fungi", "Water", "Biodiversity", "Models", " Theoretical", "Plants", "15. Life on land", "ddc:910", "Grassland", "13. Climate action", "Medicine", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0096182"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0096182", "name": "item", "description": "10.1371/journal.pone.0096182", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0096182"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-05-09T00:00:00Z"}}, {"id": "10.2134/jeq2004.0470", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:12Z", "type": "Journal Article", "created": "2005-09-09", "title": "Microbial Response To Heavy Metal-Polluted Soils: Community Analysis From Phospholipid-Linked Fatty Acids And Ester-Linked Fatty Acids Extracts", "description": "ABSTRACT<p>Heavy metal pollution of soil is of concern for human health and ecosystem function. The soil microbial community should be a sensitive indicator of metal contamination effects on bioavailability and biogeochemical processes. Simple methods are needed to determine the degree of in situ pollution and effectiveness of remediating metal\uffe2\uff80\uff90contaminated soils. Currently, phospholipid\uffe2\uff80\uff90linked fatty acids (PLFAs) are preferred for microbial profiling but this method is time consuming, whereas direct soil extraction and transesterification of total ester\uffe2\uff80\uff90linked fatty acids (ELFAs) is attractive because of its simplicity. The 1998 mining acid\uffe2\uff80\uff93metal spill of &gt;4000 ha in the Guadiamar watershed (southwestern Spain) provided a unique opportunity to study these two microbial lipid profiling methods. Replicated treatments were set up as nonpolluted, heavy metal polluted and reclaimed, and polluted soils. Inferences from whole community\uffe2\uff80\uff93diversity analysis and correlations of individual fatty acids with metals suggested Cu, Cd, and Zn were the most important in affecting microbial community structure, along with pH. The microbial stress marker, monounsaturated fatty acids, was significantly lower for reclaimed and polluted soil over nonpolluted soils for both PLFA and ELFA extraction. Another stress marker, the monounsaturated to saturated fatty acids ratio, only showed this for the PLFA. The general fungal marker (18:2\uffcf\uff896c), the arbuscule mycorrhizae marker (16:1\uffcf\uff895c), and iso\uffe2\uff80\uff90 and anteiso\uffe2\uff80\uff90branched PLFAs (Gram positive bacteria) were suppressed with increasing pollution whereas 17:0cy (Gram negative bacteria) increased with metal pollution. For both extraction methods, richness and diversity were greater in nonpolluted soils and lowest in polluted soils. The ELFA method was sensitive for reflecting metal pollution on microbial communities and could be suitable for routine use in ecological monitoring and risk assessment programs because of its simplicity and reproducibility.</p>", "keywords": ["2. Zero hunger", "Bacteria", "Fatty Acids", "Fungi", "Esters", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Soil", "Biodegradation", " Environmental", "Spain", "13. Climate action", "Metals", " Heavy", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Phospholipids", "Soil Microbiology", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.2134/jeq2004.0470"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2004.0470", "name": "item", "description": "10.2134/jeq2004.0470", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2004.0470"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-09-01T00:00:00Z"}}, {"id": "10.2139/ssrn.4106102", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:29Z", "type": "Journal Article", "created": "2022-05-28", "title": "Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil", "description": "In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation\u2019 efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic", "keywords": ["Soil enzymes", "01 natural sciences", "Total petroleum hydrocarbons polluted soils", "Soil", "Bioaugmentation", "Alkanes", "Soil Pollutants", "Micronutrients", "Polycyclic Aromatic Hydrocarbons", "Materials", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "Minerals", "Materiales", "Biostimulation", " Bioaugmentation", " Phospholipid fatty acids", " Total petroleum hydrocarbons polluted soils", " Soil enzymes", "Fatty Acids", "Phosphorus", "Phenanthrenes", "Hydrocarbons", "Phosphoric Monoester Hydrolases", "6. Clean water", "Biostimulation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Phospholipid fatty acids", "Potassium", "Oils", "Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes", "Peptide Hydrolases"]}, "links": [{"href": "https://doi.org/10.2139/ssrn.4106102"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/SSRN%20Electronic%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2139/ssrn.4106102", "name": "item", "description": "10.2139/ssrn.4106102", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2139/ssrn.4106102"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10072/428410", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:36Z", "type": "Journal Article", "created": "2023-10-18", "title": "Community composition and physiological plasticity control microbial carbon storage across natural and experimental soil fertility gradients", "description": "Abstract                <p>Many microorganisms synthesise carbon (C)-rich compounds under resource deprivation. Such compounds likely serve as intracellular C-storage pools that sustain the activities of microorganisms growing on stoichiometrically imbalanced substrates, making them potentially vital to the function of ecosystems on infertile soils. We examined the dynamics and drivers of three putative C-storage compounds (neutral lipid fatty acids [NLFAs], polyhydroxybutyrate [PHB], and trehalose) across a natural gradient of soil fertility in eastern Australia. Together, NLFAs, PHB, and trehalose corresponded to 8.5\uffe2\uff80\uff9340% of microbial C and 0.06\uffe2\uff80\uff930.6% of soil organic C. When scaled to \uffe2\uff80\uff9cstructural\uffe2\uff80\uff9d microbial biomass (indexed by polar lipid fatty acids; PLFAs), NLFA and PHB allocation was 2\uffe2\uff80\uff933-times greater in infertile soils derived from ironstone and sandstone than in comparatively fertile basalt- and shale-derived soils. PHB allocation was positively correlated with belowground biological phosphorus (P)-demand, while NLFA allocation was positively correlated with fungal PLFA : bacterial PLFA ratios. A complementary incubation revealed positive responses of respiration, storage, and fungal PLFAs to glucose, while bacterial PLFAs responded positively to PO43-. By comparing these results to a model of microbial C-allocation, we reason that NLFA primarily served the \uffe2\uff80\uff9creserve\uffe2\uff80\uff9d storage mode for C-limited taxa (i.e., fungi), while the variable portion of PHB likely served as \uffe2\uff80\uff9csurplus\uffe2\uff80\uff9d C-storage for P-limited bacteria. Thus, our findings reveal a convergence of community-level processes (i.e., changes in taxonomic composition that underpin reserve-mode storage dynamics) and intracellular mechanisms (e.g., physiological plasticity of surplus-mode storage) that drives strong, predictable community-level microbial C-storage dynamics across gradients of soil fertility and substrate stoichiometry.</p", "keywords": ["2. Zero hunger", "Science & Technology", "Ecology", "Fatty Acids", "Fungi", "Soil Science", "Trehalose", "Environmental Sciences & Ecology", "15. Life on land", "Markvetenskap", "Microbiology", "Article", "Carbon", "Environmental sciences", "Biological sciences", "Soil", "Biomass", "Life Sciences & Biomedicine", "Ecosystem", "Soil Microbiology", "Phospholipids"]}, "links": [{"href": "https://doi.org/10072/428410"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10072/428410", "name": "item", "description": "10072/428410", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10072/428410"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-18T00:00:00Z"}}, {"id": "10259/7423", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:38Z", "type": "Journal Article", "created": "2022-05-28", "title": "Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil", "description": "In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation\u2019 efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic", "keywords": ["Soil enzymes", "01 natural sciences", "Total petroleum hydrocarbons polluted soils", "Soil", "Bioaugmentation", "Alkanes", "Soil Pollutants", "Micronutrients", "Polycyclic Aromatic Hydrocarbons", "Materials", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "Minerals", "Materiales", "Fatty Acids", "Phosphorus", "Phenanthrenes", "Hydrocarbons", "Phosphoric Monoester Hydrolases", "6. Clean water", "Biostimulation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Phospholipid fatty acids", "Potassium", "Oils", "Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes", "Peptide Hydrolases"]}, "links": [{"href": "https://doi.org/10259/7423"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/SSRN%20Electronic%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10259/7423", "name": "item", "description": "10259/7423", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10259/7423"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phospholipids&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phospholipids&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phospholipids&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phospholipids&offset=23", "hreflang": "en-US"}], "numberMatched": 23, "numberReturned": 23, "distributedFeatures": [], "timeStamp": "2026-07-26T08:14:54.330971Z"}