Gypsum soils in the Mediterranean Basin house large numbers of edaphic specialists that are adapted to stressful environments. The evolutionary history and standing genetic variation of these taxa have been influenced by the geological and paleoclimatic complexity of this area and the long\uffe2\uff80\uff90standing effect of human activities. However, little is known about the origin of Mediterranean gypsophiles and the factors affecting their genetic diversity and population structure.
METHODSUsing phylogenetic and phylogeographic approaches based on microsatellites and sequence data from nuclear and chloroplast regions, we evaluated the divergence time, genetic diversity, and population structure of 27 different populations of the widespread Iberian gypsophile Lepidium subulatum throughout its entire geographic range.
RESULTSLepidium subulatum diverged from its nearest relatives ~3 million years ago, and ITS and psbA/matK trees supported the monophyly of the species. These results suggest that both geological and climatic changes in the region around the Plio\uffe2\uff80\uff90Pleistocene promoted its origin, compared to other evolutionary processes. We found high genetic diversity in both nuclear and chloroplast markers, but a greater population structure in the chloroplast data. These results suggest that while seed dispersal is limited, pollen flow may be favored by the presence of numerous habitat patches that enhance the movement of pollinators.
CONCLUSIONSDespite being an edaphic endemic, L. subulatum possesses high genetic diversity probably related to its relatively old age and high population sizes across its range. Our study highlights the value of using different markers to fully understand the phylogeographic history of plant species.The ecological drivers of soil biodiversity in the Southern Hemisphere remain underexplored. Here, in a continental survey comprising 647 sites, across 58 degrees of latitude between tropical Australia and Antarctica, we evaluated the major ecological patterns in soil biodiversity and relative abundance of ecological clusters within a co\uffe2\uff80\uff90occurrence network of soil bacteria, archaea and eukaryotes. Six major ecological clusters (modules) of co\uffe2\uff80\uff90occurring soil taxa were identified. These clusters exhibited strong shifts in their relative abundances with increasing distance from the equator. Temperature was the major environmental driver of the relative abundance of ecological clusters when Australia and Antarctica are analyzed together. Temperature, aridity, soil properties and vegetation types were the major drivers of the relative abundance of different ecological clusters within Australia. Our data supports significant reductions in the diversity of bacteria, archaea and eukaryotes in Antarctica vs. Australia linked to strong reductions in temperature. However, we only detected small latitudinal variations in soil biodiversity within Australia. Different environmental drivers regulate the diversity of soil archaea (temperature and soil carbon), bacteria (aridity, vegetation attributes and pH) and eukaryotes (vegetation type and soil carbon) across Australia. Together, our findings provide new insights into the mechanisms driving soil biodiversity in the Southern Hemisphere.
", "keywords": ["0301 basic medicine", "Terrestrial Ecosystems", "archaea", "Evolution", "Eukaryotes", "Antarctic Regions", "1105 Ecology", "Biodiversity; Terrestrial Ecosystems; Archaea; Bacteria; Eukaryotes; Australia; Antarctica.", "Terrestrial ecosystems", "Soil", "03 medical and health sciences", "eukaryotes", "Behavior and Systematics", "XXXXXX - Unknown", "14. Life underwater", "bacteria", "Phylogeny", "Soil Microbiology", "biodiversity", "2. Zero hunger", "0303 health sciences", "Bacteria", "Australia", "terrestrial ecosystems", "Biodiversity", "15. Life on land", "archaebacteria", "Archaea", "soil ecology", "13. Climate action", "eukaryotic cells", "Antarctica"]}, "links": [{"href": "https://esajournals.onlinelibrary.wiley.com/doi/pdf/10.1002/ecy.2137"}, {"href": "https://doi.org/10.1002/ecy.2137"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecy.2137", "name": "item", "description": "10.1002/ecy.2137", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecy.2137"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-12T00:00:00Z"}}, {"id": "10.1007/s00248-003-0229-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:30Z", "type": "Journal Article", "created": "2005-06-17", "title": "Methanogen Communities In A Drained Bog: Effect Of Ash Fertilization", "description": "Forestry practises such has drainage have been shown to decrease emissions of the greenhouse gas methane (CH(4)) from peatlands. The aim of the study was to examine the methanogen populations in a drained bog in northern Finland, and to assess the possible effect of ash fertilization on potential methane production and methanogen communities. Peat samples were collected from control and ash fertilized (15,000 kg/ha) plots 5 years after ash application, and potential CH(4) production was measured. The methanogen community structure was studied by DNA isolation, PCR amplification of the methyl coenzyme-M reductase (mcr) gene, denaturing gradient gel electrophoresis (DGGE), and restriction fragment length polymorphism (RFLP) analysis. The drained peatland showed low potential methane production and methanogen diversity in both control and ash-fertilized plots. Samples from both upper and deeper layers of peat were dominated by three groups of sequences related to Rice cluster-I hydrogenotroph methanogens. Even though pH was marginally greater in the ash-treated site, the occurrence of those sequences was not affected by ash fertilization. Interestingly, a less common group of sequences, related to the Fen cluster, were found only in the fertilized plots. The study confirmed the depth related change of methanogen populations in peatland.", "keywords": ["0301 basic medicine", "0303 health sciences", "Bacteria", "tuhkalannoitus", "metanogeeniset mikrobit", "Biodiversity", "15. Life on land", "03 medical and health sciences", "Genes", " Bacterial", "ojitetut suot", "Fertilizers", "Methane", "Ecosystem", "Phylogeny", "Polymorphism", " Restriction Fragment Length"]}, "links": [{"href": "https://doi.org/10.1007/s00248-003-0229-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-003-0229-2", "name": "item", "description": "10.1007/s00248-003-0229-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-003-0229-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-02-01T00:00:00Z"}}, {"id": "10.1007/s00248-013-0235-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:30Z", "type": "Journal Article", "created": "2013-05-01", "title": "Changes In Diversity, Abundance, And Structure Of Soil Bacterial Communities In Brazilian Savanna Under Different Land Use Systems", "description": "The Brazilian Savanna, also known as 'Cerrado', is the richest and most diverse savanna in the world and has been ranked as one of the main hotspots of biodiversity. The Cerrado is a representative biome in Central Brazil and the second largest biome in species diversity of South America. Nevertheless, large areas of native vegetation have been converted to agricultural land including grain production, livestock, and forestry. In this view, understanding how land use affects microbial communities is fundamental for the sustainable management of agricultural ecosystems. The aim of this work was to analyze and compare the soil bacterial communities from the Brazilian Cerrado associated with different land use systems using high throughput pyrosequencing of 16S rRNA genes. Relevant differences were observed in the abundance and structure of bacterial communities in soils under different land use systems. On the other hand, the diversity of bacterial communities was not relevantly changed among the sites studied. Land use systems had also an important impact on specific bacterial groups in soil, which might change the soil function and the ecological processes. Acidobacteria, Proteobacteria, and Actinobacteria were the most abundant groups in the Brazilian Cerrado. These findings suggest that more important than analyzing the general diversity is to analyze the composition of the communities. Since soil type was the same among the sites, we might assume that land use was the main factor defining the abundance and structure of bacterial communities.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Bacteria", "Molecular Sequence Data", "Agriculture", "Biodiversity", "15. Life on land", "Brazil", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-013-0235-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-013-0235-y", "name": "item", "description": "10.1007/s00248-013-0235-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-013-0235-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-27T00:00:00Z"}}, {"id": "10.1007/s00248-016-0730-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:30Z", "type": "Journal Article", "created": "2016-02-02", "title": "Responses Of Soil Bacterial Communities To Nitrogen Deposition And Precipitation Increment Are Closely Linked With Aboveground Community Variation", "description": "It has been predicted that precipitation and atmospheric nitrogen (N) deposition will increase in northern China; yet, ecosystem responses to the interactive effects of water and N remain largely unknown. In particular, responses of belowground microbial community to projected global change and their potential linkages to aboveground macro-organisms are rarely studied. In this study, we examined the responses of soil bacterial diversity and community composition to increased precipitation and multi-level N deposition in a temperate steppe in Inner Mongolia, China, and explored the diversity linkages between aboveground and belowground communities. It was observed that N addition caused the significant decrease in bacterial alpha-diversity and dramatic changes in community composition. In addition, we documented strong correlations of alpha- and beta-diversity between plant and bacterial communities in response to N addition. It was found that N enriched the so-called copiotrophic bacteria, but reduced the oligotrophic groups, primarily by increasing the soil inorganic N content and carbon availability and decreasing soil pH. We still highlighted that increased precipitation tended to alleviate the effects of N on bacterial diversity and dampen the plant-microbe connections induced by N. The counteractive effects of N addition and increased precipitation imply that even though the ecosystem diversity and function are predicted to be negatively affected by N deposition in the coming decades; the combination with increased precipitation may partially offset this detrimental effect.", "keywords": ["0301 basic medicine", "2. Zero hunger", "China", "0303 health sciences", "Bacteria", "Nitrogen", "Climate", "Microbial Consortia", "Water", "Biodiversity", "Plants", "15. Life on land", "Carbon", "6. Clean water", "Soil", "03 medical and health sciences", "13. Climate action", "Chemical Precipitation", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-016-0730-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-016-0730-z", "name": "item", "description": "10.1007/s00248-016-0730-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-016-0730-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-02T00:00:00Z"}}, {"id": "10.1007/s00253-019-09689-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:30Z", "type": "Journal Article", "created": "2019-02-20", "title": "Distribution of Oenococcus oeni populations in natural habitats", "description": "Oenococcus oeni is the lactic acid bacteria species most commonly encountered in wine, where it develops after the alcoholic fermentation and achieves the malolactic fermentation that is needed to improve the quality of most wines. O. oeni is abundant in the oenological environment as well as in apple cider and kombucha, whereas it is a minor species in the natural environment. Numerous studies have shown that there is a great diversity of strains in each wine region and in each product or type of wine. Recently, genomic studies have shed new light on the species diversity, population structure, and environmental distribution. They revealed that O. oeni has unique genomic features that have contributed to its fast evolution and adaptation to the enological environment. They have also unveiled the phylogenetic diversity and genomic properties of strains that develop in different regions or different products. This review explores the distribution of O. oeni and the diversity of strains in natural habitats.", "keywords": ["0106 biological sciences", "0301 basic medicine", "570", "Evolution", "[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering", "590", "Wine", "01 natural sciences", "Domestication", "Evolution", " Molecular", "03 medical and health sciences", "[SDV.IDA]Life Sciences [q-bio]/Food engineering", "MD Multidisciplinary", "[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering", "Ecosystem", "Oenococcus", "Phylogeny", "0303 health sciences", "Malolactic fermentation", "Genetic Variation", "Genomics", "[SDV.IDA] Life Sciences [q-bio]/Food engineering", "Mini-Review", "Fermentation", "Oenococcus oeni", "Biotechnology"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00253-019-09689-z.pdf"}, {"href": "https://doi.org/10.1007/s00253-019-09689-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Microbiology%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00253-019-09689-z", "name": "item", "description": "10.1007/s00253-019-09689-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00253-019-09689-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-20T00:00:00Z"}}, {"id": "10.1007/s00253-016-7736-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:30Z", "type": "Journal Article", "created": "2016-07-27", "title": "Structure Of Bacterial Communities In Soil Following Cover Crop And Organic Fertilizer Incorporation", "description": "Incorporation of organic material into soils is an important element of organic farming practices that can affect the composition of the soil bacterial communities that carry out nutrient cycling and other functions crucial to crop health and growth. We conducted a field experiment to determine the effects of cover crops and fertilizers on bacterial community structure in agricultural soils under long-term organic management. Illumina sequencing of 16S rDNA revealed diverse communities comprising 45 bacterial phyla in corn rhizosphere and bulk field soil. Community structure was most affected by location and by the rhizosphere effect, followed by sampling time and amendment treatment. These effects were associated with soil physicochemical properties, including pH, moisture, organic matter, and nutrient levels. Treatment differences were apparent in bulk and rhizosphere soils at the time of peak corn growth in the season following cover crop and fertilizer application. Cover crop and fertilizer treatments tended to lower alpha diversity in early season samples. However, winter rye, oilseed radish, and buckwheat cover crop treatments increased alpha diversity in some later season samples compared to a no-amendment control. Fertilizer treatments and some cover crops decreased relative abundance of members of the ammonia-oxidizing family Nitrosomonadaceae. Pelleted poultry manure and Sustane\u00ae (a commercial fertilizer) decreased the relative abundance of Rhizobiales. Our data point to a need for future research exploring how (1) cover crops influence bacterial community structure and functions, (2) these effects differ with biomass composition and quantity, and (3) existing soil conditions and microbial community composition influence how soil microbial populations respond to agricultural management practices.", "keywords": ["Crops", " Agricultural", "DNA", " Bacterial", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "High-Throughput Nucleotide Sequencing", "Sequence Analysis", " DNA", "15. Life on land", "Biota", "DNA", " Ribosomal", "03 medical and health sciences", "13. Climate action", "RNA", " Ribosomal", " 16S", "Fertilizers", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00253-016-7736-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Microbiology%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00253-016-7736-9", "name": "item", "description": "10.1007/s00253-016-7736-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00253-016-7736-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-07-27T00:00:00Z"}}, {"id": "10.1007/s00442-017-3965-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:41Z", "type": "Journal Article", "created": "2017-10-05", "title": "Nutrient Limitation Of Soil Microbial Activity During The Earliest Stages Of Ecosystem Development", "description": "A dominant paradigm in ecology is that plants are limited by nitrogen (N) during primary succession. Whether generalizable patterns of nutrient limitation are also applicable to metabolically and phylogenetically diverse soil microbial communities, however, is not well understood. We investigated if measures of N and phosphorus (P) pools inform our understanding of the nutrient(s) most limiting to soil microbial community activities during primary succession. We evaluated soil biogeochemical properties and microbial processes using two complementary methodological approaches-a nutrient addition microcosm experiment and extracellular enzyme assays-to assess microbial nutrient limitation across three actively retreating glacial chronosequences. Microbial respiratory responses in the microcosm experiment provided evidence for N, P and N/P co-limitation at Easton Glacier, Washington, USA, Puca Glacier, Peru, and Mendenhall Glacier, Alaska, USA, respectively, and patterns of nutrient limitation generally reflected site-level differences in soil nutrient availability. The activities of three key extracellular enzymes known to vary with soil N and P availability developed in broadly similar ways among sites, increasing with succession and consistently correlating with changes in soil total N pools. Together, our findings demonstrate that during the earliest stages of soil development, microbial nutrient limitation and activity generally reflect soil nutrient supply, a result that is broadly consistent with biogeochemical theory.", "keywords": ["Washington", "2. Zero hunger", "Nitrogen", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Soil", "Food", "Peru", "0401 agriculture", " forestry", " and fisheries", "Ice Cover", "Alaska", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00442-017-3965-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-017-3965-6", "name": "item", "description": "10.1007/s00442-017-3965-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-017-3965-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-10-05T00:00:00Z"}}, {"id": "10.1007/s00572-016-0694-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:42Z", "type": "Journal Article", "created": "2016-04-14", "title": "Organic Amendments Increase Phylogenetic Diversity Of Arbuscular Mycorrhizal Fungi In Acid Soil Contaminated By Trace Elements", "description": "In 1998, a toxic mine spill polluted a 55-km(2) area in a basin southward to Do\u00f1ana National Park (Spain). Subsequent attempts to restore those trace element-contaminated soils have involved physical, chemical, or biological methodologies. In this study, the restoration approach included application of different types and doses of organic amendments: biosolid compost (BC) and leonardite (LEO). Twelve years after the last addition, molecular analyses of arbuscular mycorrhizal (AM) fungal communities associated with target plants (Lamarckia aurea and Chrysanthemum coronarium) as well as analyses of trace element concentrations both in soil and in plants were performed. The results showed an improved soil quality reflected by an increase in soil pH and a decrease in trace element availability as a result of the amendments and dosages. Additionally, the phylogenetic diversity of the AM fungal community increased, reaching the maximum diversity at the highest dose of BC. Trace element concentration was considered the predominant soil factor determining the AM fungal community composition. Thereby, the studied AM fungal community reflects a community adapted to different levels of contamination as a result of the amendments. The study highlights the long-term effect of the amendments in stabilizing the soil system.", "keywords": ["2. Zero hunger", "0301 basic medicine", "Minerals", "0303 health sciences", "Bioindicator", "Chrysanthemum", "Genetic Variation", "Hydrogen-Ion Concentration", "15. Life on land", "Poaceae", "Soil biodiversity", "Trace element contaminated soils", "Ecosystem restoration", "Mining", "Soil fungal community", "Trace Elements", "Soil", "03 medical and health sciences", "Biodegradation", " Environmental", "13. Climate action", "Mycorrhizae", "Mine spill", "Bioindicators", "Soil Pollutants", "Phylogeny"]}, "links": [{"href": "https://doi.org/10.1007/s00572-016-0694-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00572-016-0694-3", "name": "item", "description": "10.1007/s00572-016-0694-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00572-016-0694-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-04-12T00:00:00Z"}}, {"id": "10.1007/s10265-009-0294-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:14:47Z", "type": "Journal Article", "created": "2010-01-12", "title": "Taxonomic Identity, Phylogeny, Climate And Soil Fertility As Drivers Of Leaf Traits Across Chinese Grassland Biomes", "description": "Although broad-scale inter-specific patterns of leaf traits are influenced by climate, soil, and taxonomic identity, integrated assessments of these drivers remain rare. Here, we quantify these drivers in a field study of 171 plant species in 174 sites across Chinese grasslands, including the Tibetan Plateau, Inner Mongolia, and Xinjiang. General linear models were used to partition leaf trait variation. Of the total variation in leaf traits, on average 27% is due to taxonomic or phylogenetic differences among species within sites (pure species effect), 29% to variation among sites within species (pure site effect), 38% to joint effects of taxonomic and environmental factors (shared effect), and 6.2% to within-site and within-species variation. Examining the pure site effect, climate explained 7.8%, soil explained 7.4%, and climate and soil variables together accounted for 11%, leaving 18% of the inter-site variation due to factors other than climate or soil. The results do not support the hypothesis that soil fertility is the 'missing link' to explain leaf trait variation unexplained by climatic factors. Climate- and soil-induced leaf adaptations occur mostly among species, and leaf traits vary little within species in Chinese grassland plants, despite strongly varying climate and soil conditions.", "keywords": ["0106 biological sciences", "China", "Climate", "Soil fertility", "Poaceae", "01 natural sciences", "10127 Institute of Evolutionary Biology and Environmental Studies", "Soil", "Quantitative Trait", " Heritable", "Species Specificity", "1110 Plant Science", "Tibetan Plateau", "Leaf economics spectrum", "functional traits", "Photosynthesis", "Ecosystem", "Phylogeny", "2. Zero hunger", "photosynthesis", "soil fertility", "Inner Mongolia (China)", "15. Life on land", "Plant Leaves", "Inner Mongolia", "Linear Models", "leaf economics", "570 Life sciences; biology", "590 Animals (Zoology)", "Functional traits"]}, "links": [{"href": "https://doi.org/10.1007/s10265-009-0294-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Plant%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10265-009-0294-9", "name": "item", "description": "10.1007/s10265-009-0294-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10265-009-0294-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-01-13T00:00:00Z"}}, {"id": "10.1007/s11274-011-0809-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:15:17Z", "type": "Journal Article", "created": "2011-06-15", "title": "Soil Bacterial Community Composition And Diversity Respond To Cultivation In Karst Ecosystems", "description": "Soil microorganisms play vital roles in recovering and maintaining the health of ecosystems, particularly in fragile Karst ecosystems that are easily degraded after cultivation. We investigated the composition and diversity of soil bacterial communities, based on RFLP and 16S rDNA sequencing, in a cropland, a naturally revegetated land with former cultivation disturbance and a primeval forest in the subtropical Karst of southwest China. Our results illustrated that Proteobacteria accounted for 44.8% of the 600 tested clones, making it the most dominant phylum observed. This phylum was followed by Acidobacteria and Planctomycetes for the three Karst soils analyzed. Compared with the primeval forest soil, the proportions of Proteobacteria were decreased by 30.2 and 37.9%, while Acidobacteria increased by 93.9 and 87.9%, and the Shannon-Wiener diversity indices and the physicochemical parameters declined in the cropland and the revegetated land, respectively. Among the three soils, the proportion of dominant bacterial phyla and the diversity indices in the revegetated land were similar to the cropland, implying the bacterial community in the cropland was relatively stable, and the after-effects of cultivation were difficult to eliminate. However, similar distributions of the four Proteobacteria subphyla were observed between the revegetated land and the primeval forest soil. Furthermore, the proportion of Rhizobiales belonging to \u03b1-Proteobacteria was sharply decreased with cultivation compared to the primeval forest soil, while a small cluster of Rhizobiales recurred with vegetation recovery. These results indicated that although the subphyla of the dominant bacterial phylum had some positive responses to 20\u00a0years of vegetation recovery, it is a slow process. Our results suggest that priority should be given to conserve the primeval forest and inoculation of functional microorganisms on the basis of vegetation recovery may be more effective for the restoration of Karst ecosystems after cultivation.", "keywords": ["2. Zero hunger", "0301 basic medicine", "China", "0303 health sciences", "Bacteria", "Agriculture", "Biodiversity", "15. Life on land", "Trees", "RNA", " Bacterial", "Soil", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "Metagenome", "Ecosystem", "Phylogeny", "Polymorphism", " Restriction Fragment Length", "Soil Microbiology"], "contacts": [{"organization": "Xiangbi Chen, Ya-wei Wei, Wenxue Wei, Jinshui Wu, Yirong Su, Xunyang He,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s11274-011-0809-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/World%20Journal%20of%20Microbiology%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11274-011-0809-0", "name": "item", "description": "10.1007/s11274-011-0809-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11274-011-0809-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-06-15T00:00:00Z"}}, {"id": "10.1007/s12275-012-2409-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:15:24Z", "type": "Journal Article", "created": "2012-11-03", "title": "Characterization Of The Bacterial And Archaeal Communities In Rice Field Soils Subjected To Long-Term Fertilization Practices", "description": "The bacterial and archaeal communities in rice field soils subjected to different fertilization regimes for 57 years were investigated in two different seasons, a non-planted, drained season (April) and a rice-growing, flooded season (August), by performing soil dehydrogenase assay, real-time PCR assay and pyrosequencing analysis. All fertilization regimes increased the soil dehydrogenase activity while the abundances of bacteria and archaea increased in the plots receiving inorganic fertilizers plus compost and not in those receiving inorganic fertilizers only. Rice-growing and flooding decreased the soil dehydrogenase activity while they increased the bacterial diversity in rice field soils. The bacterial communities were dominated by Chloroflexi, Proteobacteria, and Actinobacteria and the archaeal communities by Crenarchaeota at the phylum level. In principal coordinates analysis based on the weighted Fast UniFrac metric, the bacterial and archaeal communities were separated primarily by season, and generally distributed along with soil pH, the variation of which had been caused by long-term fertilization. Variations in the relative abundance according to the season or soil pH were observed for many bacterial and archaeal groups. In conclusion, the microbial activity, prokaryotic abundance and diversity, and prokaryotic community structure in the rice field soils were changed by season and long-term fertilization.", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Molecular Sequence Data", "Agriculture", "Oryza", "15. Life on land", "Archaea", "6. Clean water", "Soil", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "Seasons", "Fertilizers", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s12275-012-2409-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s12275-012-2409-6", "name": "item", "description": "10.1007/s12275-012-2409-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s12275-012-2409-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-10-01T00:00:00Z"}}, {"id": "10.1016/j.cryobiol.2024.104859", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:16:07Z", "type": "Journal Article", "created": "2024-02-07", "title": "Cryopreservation and recovery of a complex hypersaline microbial mat community", "description": "Cryopreservation of microorganisms is an essential tool in industrial- and food applications where conservation of microbial activity and critical beneficial traits need to be guaranteed to provide a consistent product or production process. This often refers to simple, single species or low diversity assemblages in liquid cultures that can easily be revived and regrown to perform the desired process. Cryopreservation is also of essence for scientific experimentation where many environmental samples are taken in remote sampling sites and at high costs. Biobanking, or the long term preservation and potential revival of complex, structured samples come with an additional challenge related to maintaining the structure upon revival. Here we look at cryopreserving and reviving a complex photosynthesis driven microbial mat from a hypersaline ecosystem. Amplicon sequencing of the 16S and 18S ribosomal RNA gene was used to determine the community composition of bacteria and eukaryotes respectively. The tests included the use of different cryopreservative agents and different times of cryopreservation at -150\u00a0\u00b0C. Upon revival, the cryopreservatives cannot be separated from the preserved samples without disturbing the community structure, while carryover of these compounds may influence reconstitution of the communities. Indeed, although both glycerol and Me2SO are good cryopreservatives of microbial assemblages, carryover of these compounds had a profound negative effect on the reestablishment of a functional microbial mat. Best cryopreservation and reconstitution results were obtained in the absence of a cryopreservative agent or when methanol was used.", "keywords": ["Cryopreservation", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Bacteria", "Photosynthesis", "Ecosystem", "Phylogeny", "6. Clean water", "Biological Specimen Banks"]}, "links": [{"href": "https://doi.org/10.1016/j.cryobiol.2024.104859"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Cryobiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cryobiol.2024.104859", "name": "item", "description": "10.1016/j.cryobiol.2024.104859", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cryobiol.2024.104859"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2010.04.017", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:16:20Z", "type": "Journal Article", "created": "2010-05-25", "title": "Molecular Diversity Of Arbuscular Mycorrhizal Fungi In Relation To Soil Chemical Properties And Heavy Metal Contamination", "description": "Abundance and diversity of arbuscular mycorrhizal fungi (AMF) associated with dominant plant species were studied along a transect from highly lead (Pb) and zinc (Zn) polluted to non-polluted soil at the Anguran open pit mine in Iran. Using an established primer set for AMF in the internal transcribed spacer (ITS) region of rDNA, nine different AMF sequence types were distinguished after phylogenetic analyses, showing remarkable differences in their distribution patterns along the transect. With decreasing Pb and Zn concentration, the number of AMF sequence types increased, however one sequence type was only found in the highly contaminated area. Multivariate statistical analysis revealed that further factors than HM soil concentration affect the AMF community at contaminated sites. Specifically, the soils' calcium carbonate equivalent and available P proved to be of importance, which illustrates that field studies on AMF distribution should also consider important environmental factors and their possible interactions.", "keywords": ["0301 basic medicine", "Soil", "Zinc", "03 medical and health sciences", "Lead", "13. Climate action", "Mycorrhizae", "Soil Pollutants", "Biodiversity", "15. Life on land", "01 natural sciences", "Phylogeny", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2010.04.017"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2010.04.017", "name": "item", "description": "10.1016/j.envpol.2010.04.017", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2010.04.017"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-01T00:00:00Z"}}, {"id": "10.1016/j.ijbiomac.2021.10.032", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:16:45Z", "type": "Journal Article", "created": "2021-10-14", "title": "Identification and molecular characterization of the high-affinity copper transporters family in Solanum lycopersicum.", "description": "Copper (Cu) plays a key role as cofactor in the plant proteins participating in essential cellular processes, such as electron transport and free radical scavenging. Despite high-affinity Cu transporters (COPTs) being key participants in Cu homeostasis maintenance, very little is known about COPTs in tomato (Solanum lycopersicum) even though it is the most consumed fruit worldwide and this crop is susceptible to suboptimal Cu conditions. In this study, a six-member family of COPT (SlCOPT1-6) was identified and characterized. SlCOPTs have a conserved architecture consisting of three transmembrane domains and \u03b2-strains. However, the presence of essential methionine residues, a methionine-enriched amino-terminal region, an Mx3Mx12Gx3G Cu-binding motif and a cysteine rich carboxy-terminal region, all required for their functionality, is more variable among members. Accordingly, functional complementation assays in yeast indicate that SlCOPT1 and SlCOPT2 are able to transport Cu inside the cell, while SlCOPT3 and SlCOPT5 are only partially functional. In addition, protein interaction network analyses reveal the connection between SlCOPTs and Cu PIB-type ATPases, other metal transporters, and proteins related to the peroxisome. Gene expression analyses uncover organ-dependency, fruit vasculature tissue specialization and ripening-dependent gene expression profiles, as well as different response to Cu deficiency or toxicity in an organ-dependent manner.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Biotecnologia agr\u00edcola", "Molecular Conformation", "COPT", "Gene Expression", "Tomato", "Structure-Activity Relationship", "03 medical and health sciences", "Copper Transport Proteins", "Solanum lycopersicum", "Multigene Family", "Tom\u00e0quets", "Amino Acid Sequence", "Heavy metal stress", "Conserved Sequence", "Copper", "Phylogeny", "Plant Proteins"]}, "links": [{"href": "https://doi.org/10.1016/j.ijbiomac.2021.10.032"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Biological%20Macromolecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijbiomac.2021.10.032", "name": "item", "description": "10.1016/j.ijbiomac.2021.10.032", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijbiomac.2021.10.032"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-01T00:00:00Z"}}, {"id": "10.1016/j.mib.2019.08.004", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:16:57Z", "type": "Journal Article", "created": "2019-10-22", "title": "The mycobiota: fungi take their place between plants and bacteria", "description": "Eukaryotes host numerous intracellular and associated microbes in their microbiota. Fungi, the so-called Mycobiota, are important members of both human and plant microbiota. Moreover, members of the plant mycobiota host their own microbiota on their surfaces and inside their hyphae. The microbiota of the mycobiota includes mycorrhizal helper bacteria (for mycorrhizal fungi) and fungal endobacteria, which are critical for the fungal host and, as such, likely affect the plant. This review discusses the contribution that these often-overlooked members make to the composition and performance of the plant microbiota.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Bacteria", "Host Microbial Interactions", "RNA", " Ribosomal", " 16S", "Fungi", "Microbial Interactions", "Plants", "Bacterial Physiological Phenomena", "Symbiosis", "Phylogeny", "Mycobiome"], "contacts": [{"organization": "Bonfante P., Venice F., Lanfranco L.,", "roles": ["creator"]}]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/1728500/7/Bonfante%2c%20Venice%20and%20Lanfranco_Current%20Opinion%20Author%20final%20version.pdf"}, {"href": "https://doi.org/10.1016/j.mib.2019.08.004"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Current%20Opinion%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.mib.2019.08.004", "name": "item", "description": "10.1016/j.mib.2019.08.004", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.mib.2019.08.004"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1016/j.plaphy.2019.05.023", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:17:00Z", "type": "Journal Article", "created": "2019-05-29", "title": "The elemental composition of halophytes correlates with key morphological adaptations and taxonomic groups", "description": "Halophytes are crucial in the light of increasing soil salinization, yet our understanding of their chemical composition and its relationship to key morphological traits such as succulence or salt excretion is limited. This study targets this issue by exploring the relationship between the elemental composition of 108 plant species from saline environments in Iran and their eco-morphological traits and taxonomy. Leaves and/or photosynthetic shoots of individual species and soils were sampled and analyzed for 20 elements in plant samples and 5 major elements plus % gypsum content, pH, and EC in soil samples. Eu-halophytes and leaf- and stem-succulent and salt-recreting plants showed high concentrations of Na, S, and Mg and low concentrations of Ca and K. In contrast, pseudo-halophytes, facultative-halophytes and eury-hygro-halophytes, which often lack succulent shoots, showed low Na, S, and Mg and high Ca and K concentrations in their leaves. Clear patterns were identified among taxonomic families, with Chenopodiaceae and Plumbaginaceae having high Na and Mg and low Ca and K concentrations, Caryophyllaceae having high K, Poaceae having low Na, and Asteraceae, Boraginaceae, and Brassicaceae showing high foliar Ca concentrations. We conclude that the elemental composition of halophytes and pseudo-halophytes is related to salt-tolerance categories, eco-morphological types and respective taxonomic groups.", "keywords": ["Succulent halophytes", "0106 biological sciences", "0301 basic medicine", "Cytoplasm", "Salinity", "Persian Gulf", "Climate", "Chenopodiaceae", "Iran", "Plant Roots", "01 natural sciences", "Ionome", "Soil", "03 medical and health sciences", "Magnesium", "Recreting halophytes", "Ecosystem", "Phylogeny", "Geography", "Lake Urmia", "Salt-Tolerant Plants", "Hydrogen-Ion Concentration", "15. Life on land", "Adaptation", " Physiological", "Caryophyllales", "Plant Leaves", "Calcium", "Sulfur"]}, "links": [{"href": "https://doi.org/10.1016/j.plaphy.2019.05.023"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.plaphy.2019.05.023", "name": "item", "description": "10.1016/j.plaphy.2019.05.023", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.plaphy.2019.05.023"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2009.08.045", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:17:04Z", "type": "Journal Article", "created": "2009-09-23", "title": "Application Of Temporal Temperature Gradient Gel Electrophoresis For Characterisation Of Fungal Endophyte Communities Of Salix Caprea L. In A Heavy Metal Polluted Soil", "description": "Fungal endophytes can affect the heavy metal uptake of their host plants and increase the tolerance of their host plants to heavy metal stress. Therefore, in the present study, a wide-range screening of the fungal endophyte communities was conducted to determine the fungal distribution and diversity on S. caprea roots on a metal polluted site. Fungal communities were screened using amplification with the 5.8S-ITS2-28S part of the rDNA operon, with the resulting amplicons analysed by temporal temperature gradient gel electrophoresis (TTGE) and sequencing. This technique is reproducible and shows good coverage of ascomycete and basidiomycete taxa, as 68% and 32% of all of the sequences, respectively. No clear shift in fungal ITS-TTGE profiles from S. caprea roots was seen along the secondary succession stages. Ascomycetes dominated the more polluted plots, while there was a greater diversity of basidiomycetes in the less polluted and control plots, suggesting greater tolerance of ascomycetes in comparison with basidiomycete fungi. The high diversity of DSEs was confirmed at the highly metal-enriched locations, with species belonging to the genera Phialophora, Phialocephala and Leptodontidium. Furthermore, the DSE colonisation of S. caprea roots and the frequency of the sequences showing affinity towards DSE genus Phialophora, showed good correspondence with soil Pb, Cd and plant-available P concentrations, possibly indicating that DSEs improve metal tolerance of willows to high heavy metal contamination.", "keywords": ["Electrophoresis", " Agar Gel", "0301 basic medicine", "dark septate endophytes", "Fungi", "Temperature", "mycorrhiza", "Salix", "04 agricultural and veterinary sciences", "info:eu-repo/classification/udc/581", "15. Life on land", "heavy metal pollution", "community fingerprinting", "Soil", "03 medical and health sciences", "13. Climate action", "Metals", " Heavy", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "DNA", " Fungal", "Phylogeny", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2009.08.045"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2009.08.045", "name": "item", "description": "10.1016/j.scitotenv.2009.08.045", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2009.08.045"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-12-01T00:00:00Z"}}, {"id": "10.1038/s41579-020-0402-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:15Z", "type": "Journal Article", "created": "2020-07-21", "title": "Unique and common traits in mycorrhizal symbioses", "description": "Mycorrhizas are among the most important biological interkingdom interactions, as they involve ~340,000 land plants and ~50,000 taxa of soil fungi. In these mutually beneficial interactions, fungi receive photosynthesis-derived carbon and provide the host plant with mineral nutrients such as phosphorus and nitrogen in exchange. More than 150 years of research on mycorrhizas has raised awareness of their biology, biodiversity and ecological impact. In this Review, we focus on recent phylogenomic, molecular and cell biology studies to present the current state of knowledge of the origin of mycorrhizal fungi and the evolutionary history of their relationship with land plants. As mycorrhizas feature a variety of phenotypes, depending on partner taxonomy, physiology and cellular interactions, we explore similarities and differences between mycorrhizal types. During evolution, mycorrhizal fungi have refined their biotrophic capabilities to take advantage of their hosts as food sources and protective niches, while plants have developed multiple strategies to accommodate diverse fungal symbionts. Intimate associations with pervasive ecological success have originated at the crossroads between these two evolutionary pathways. Our understanding of the biological processes underlying these symbioses, where fungi act as biofertilizers and bioprotectors, provides the tools to design biotechnological applications addressing environmental and agricultural challenges.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Mycorrhizae", "15. Life on land", "Symbiosis", "Biological Evolution", "Phylogeny", "Signal Transduction"]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/1758325/1/NatRevpreprint.pdf"}, {"href": "http://www.nature.com/articles/s41579-020-0402-3.pdf"}, {"href": "https://doi.org/10.1038/s41579-020-0402-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Reviews%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41579-020-0402-3", "name": "item", "description": "10.1038/s41579-020-0402-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41579-020-0402-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-21T00:00:00Z"}}, {"id": "10.1016/j.syapm.2020.126149", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:17:40Z", "type": "Journal Article", "created": "2020-09-30", "title": "Genetic diversity and phylogeny of indigenous rhizobia nodulating faba bean (Vicia faba L.) in Greece", "description": "The genetic diversity and phylogeny of fast-growing rhizobia isolated from root nodules of Vicia faba grown in different geographical regions of Greece were assessed. Although Rhizobium leguminosarum sv. viciae is the most common symbiont of Vicia spp. in European soils, there is no available information on native rhizobia nodulating faba bean in Greece. Seventy bacterial strains were isolated and grouped into sixteen distinct profiles based on BOX-PCR fingerprinting. The phylogenetic affiliation was further defined by sequence analysis of the rrs and multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and gyrB). Fifty-eight isolates were affiliated with recently described genospecies gsF-2, represented by R. laguerreae FB206T, whereas six isolates were closely related to gsB and two isolates might belong to gsA. Two isolates assigned to R. hidalgonense and another two non-nodulating strains could not be assigned to any validly defined species and possibly belong to a new rhizobial lineage. Interestingly, R. laguerreae strains were commonly found at all sampling sites, suggesting that they could be the main symbionts of faba beans in Greek soils. According to the phylogenies of two symbiosis-related genes (nodC and nifH), all nodulating isolates belonged to symbiovar (sv.) viciae harboring four distinct nodC gene haplotypes and they were grouped into two clades together with strains assigned to R. laguerreae and genospecies of R. leguminosarum isolated from other countries and continents. This is the first report that R. hidalgonense strains belong to sv. viciae. No correlation was observed between the nodC haplotypes, geographic origin and chromosomal background of the isolates in the study.", "keywords": ["MLSA", "DNA", " Bacterial", "0301 basic medicine", "0303 health sciences", "Genes", " Essential", "Greece", "Viciae", "Sequence Analysis", " DNA", "DNA Fingerprinting", "Vicia faba", "03 medical and health sciences", "Symbiovar", "Genes", " Bacterial", "RNA", " Ribosomal", " 16S", "Root Nodules", " Plant", "Symbiosis", "Phylogeny", "Soil Microbiology", "Multilocus Sequence Typing", "Rhizobium"]}, "links": [{"href": "https://doi.org/10.1016/j.syapm.2020.126149"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Systematic%20and%20Applied%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.syapm.2020.126149", "name": "item", "description": "10.1016/j.syapm.2020.126149", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.syapm.2020.126149"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-01T00:00:00Z"}}, {"id": "10.1016/j.syapm.2012.10.007", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:17:40Z", "type": "Journal Article", "created": "2013-01-18", "title": "Shifts In Soil Bacterial Community After Eight Years Of Land-Use Change", "description": "The interaction between plants, soil and microorganisms is considered to be the major driver of ecosystem functions and any modification of plant cover and/or soil properties might affect the microbial structure, which, in turn, will influence ecological processes. Assuming that soil properties are the major drivers of soil bacterial diversity and structure within the same soil type, it can be postulated whether plant cover causes significant shifts in soil bacterial community composition. To address this question, this study used 16S rRNA pyrosequencing to detect differences in diversity, composition and/or relative abundance of bacterial taxa from an area covered by pristine forest, as well as eight-year-old grassland surrounded by the same forest. It was shown that a total of 69% of the operational taxonomic units (OTUs) were shared between environments. Overall, forest and grassland samples presented the same diversity and the clustering analysis did not show the occurrence of very distinctive bacterial communities between environments. However, 11 OTUs were detected in statistically significant higher abundance in the forest samples but in lower abundance in the grassland samples, whereas 12 OTUs occurred in statistically significant higher abundance in the grassland samples but in lower abundance in the forest samples. The results suggested the prevalence of a resilient core microbial community that did not suffer any change related to land use, soil type or edaphic conditions. The results illustrated that the history of land use might influence present-day community structure.", "keywords": ["DNA", " Bacterial", "2. Zero hunger", "Agriculture", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "Biota", "DNA", " Ribosomal", "Trees", "13. Climate action", "RNA", " Ribosomal", " 16S", "Cluster Analysis", "0401 agriculture", " forestry", " and fisheries", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.syapm.2012.10.007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Systematic%20and%20Applied%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.syapm.2012.10.007", "name": "item", "description": "10.1016/j.syapm.2012.10.007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.syapm.2012.10.007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-03-01T00:00:00Z"}}, {"id": "10.1038/s41396-021-01064-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:11Z", "type": "Journal Article", "created": "2021-07-27", "title": "Ammonia-oxidizing archaea possess a wide range of cellular ammonia affinities", "description": "AbstractNitrification, the oxidation of ammonia to nitrate, is an essential process in the biogeochemical nitrogen cycle. The first step of nitrification, ammonia oxidation, is performed by three, often co-occurring guilds of chemolithoautotrophs: ammonia-oxidizing bacteria (AOB), archaea (AOA), and complete ammonia oxidizers (comammox). Substrate kinetics are considered to be a major niche-differentiating factor between these guilds, but few AOA strains have been kinetically characterized. Here, the ammonia oxidation kinetic properties of 12 AOA representing all major cultivated phylogenetic lineages were determined using microrespirometry. Members of the genus Nitrosocosmicus have the lowest affinity for both ammonia and total ammonium of any characterized AOA, and these values are similar to previously determined ammonia and total ammonium affinities of AOB. This contrasts previous assumptions that all AOA possess much higher substrate affinities than their comammox or AOB counterparts. The substrate affinity of ammonia oxidizers correlated with their cell surface area to volume ratios. In addition, kinetic measurements across a range of pH values supports the hypothesis that\uffe2\uff80\uff94like for AOB\uffe2\uff80\uff94ammonia and not ammonium is the substrate for the ammonia monooxygenase enzyme of AOA and comammox. Together, these data will facilitate predictions and interpretation of ammonia oxidizer community structures and provide a robust basis for establishing testable hypotheses on competition between AOB, AOA, and comammox.Global climate models project a decrease in the magnitude of precipitation in tropical regions. Changes in rainfall patterns have important implications for the moisture content and redox status of tropical soils, yet little is known about how these changes may affect microbial community structure. Specifically, does exposure to prior stress confer increased resistance to subsequent perturbation? Here we reduced the quantity of precipitation throughfall to tropical forest soils in the Luquillo Mountains, Puerto Rico. Treatments included newly established throughfall exclusion plots (de novo excluded), plots undergoing reduction for a second time (pre-excluded) and ambient control plots. Ten months of throughfall exclusion led to a small but statistically significant decline in soil water potential and bacterial populations clearly adapted to increased osmotic stress. Although the water potential decline was small and microbial biomass did not change, phylogenetic diversity in the de novo-excluded plots decreased by \uffe2\uff88\uffbc40% compared with the control plots, yet pre-excluded plots showed no significant change. On the other hand, the relative abundances of bacterial taxa in both the de novo-excluded and pre-excluded plots changed significantly with throughfall exclusion compared with control plots. Changes in bacterial community structure could be explained by changes in soil pore water chemistry and suggested changes in soil redox. Soluble iron declined in treatment plots and was correlated with decreased soluble phosphorus concentrations, which may have significant implications for microbial productivity in these P-limited systems.
", "keywords": ["0301 basic medicine", "2. Zero hunger", "Tropical Climate", "0303 health sciences", "Bacteria", "Rain", "Puerto Rico", "Water", "Phosphorus", "15. Life on land", "6. Clean water", "Droughts", "Trees", "Soil", "03 medical and health sciences", "13. Climate action", "Biomass", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1038/ismej.2012.113"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/ismej.2012.113", "name": "item", "description": "10.1038/ismej.2012.113", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/ismej.2012.113"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-11-15T00:00:00Z"}}, {"id": "10.1038/nature02053", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:09Z", "type": "Journal Article", "created": "2003-10-22", "title": "Genome-Scale Approaches To Resolving Incongruence In Molecular Phylogenies", "description": "One of the most pervasive challenges in molecular phylogenetics is the incongruence between phylogenies obtained using different data sets, such as individual genes. To systematically investigate the degree of incongruence, and potential methods for resolving it, we screened the genome sequences of eight yeast species and selected 106 widely distributed orthologous genes for phylogenetic analyses, singly and by concatenation. Our results suggest that data sets consisting of single or a small number of concatenated genes have a significant probability of supporting conflicting topologies. By contrast, analyses of the entire data set of concatenated genes yielded a single, fully resolved species tree with maximum support. Comparable results were obtained with a concatenation of a minimum of 20 genes; substantially more genes than commonly used but a small fraction of any genome. These results have important implications for resolving branches of the tree of life.", "keywords": ["0301 basic medicine", "Saccharomyces", "0303 health sciences", "03 medical and health sciences", "Genes", " Fungal", "Regression Analysis", "Reproducibility of Results", "Genomics", "Genome", " Fungal", "Phylogeny"]}, "links": [{"href": "https://doi.org/10.1038/nature02053"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/nature02053", "name": "item", "description": "10.1038/nature02053", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/nature02053"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-10-01T00:00:00Z"}}, {"id": "10.1038/s41396-019-0465-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:10Z", "type": "Journal Article", "created": "2019-06-27", "title": "Plant-driven niche differentiation of ammonia-oxidizing bacteria and archaea in global drylands", "description": "AbstractUnder controlled laboratory conditions, high and low ammonium availability are known to favor soil ammonia-oxidizing bacteria (AOB) and archaea (AOA) communities, respectively. However, whether this niche segregation is maintained under field conditions in terrestrial ecosystems remains unresolved, particularly at the global scale. We hypothesized that perennial vegetation might favor AOB vs. AOA communities compared with adjacent open areas devoid of perennial vegetation (i.e., bare soil) via several mechanisms, including increasing the amount of ammonium in soil. To test this niche-differentiation hypothesis, we conducted a global field survey including 80 drylands from 6 continents. Data supported our hypothesis, as soils collected under plant canopies had higher levels of ammonium, as well as higher richness (number of terminal restriction fragments; T-RFs) and abundance (qPCR amoA genes) of AOB, and lower richness and abundance of AOA, than those collected in open areas located between plant canopies. Some of the reported associations between plant canopies and AOA and AOB communities can be a consequence of the higher organic matter and available N contents found under plant canopies. Other aspects of soils associated with vegetation including shading and microclimatic conditions might also help explain our results. Our findings provide strong evidence for niche differentiation between AOA and AOB communities in drylands worldwide, advancing our understanding of their ecology and biogeography at the global scale.Although the role played by phylogeny in the assembly of plant communities remains as a priority to complete the theory of species coexistence, experimental evidence is lacking. It is still unclear to what extent phylogenetic diversity is a driver or a consequence of species assembly processes. We experimentally explored how phylogenetic diversity can drive the community level responses to drought conditions in annual plant communities. We manipulated the initial phylogenetic diversity of the assemblages and the water availability in a common garden experiment with two irrigation treatments: average natural rainfall and drought, formed with annual plant species of gypsum ecosystems of Central Spain. We recorded plant survival and the numbers of flowering and fruiting plants per species in each assemblage. GLMMs were performed for the proportion of surviving, flowering, fruiting plants per species and for total proportion of surviving species and plants per pot. In water limited conditions, high phylogenetic diversity favored species coexistence over time with higher plant survival and more flowering and fruiting plants per species and more species and plants surviving per pot. Our results agree with the existence of niche complementarity and the convergence of water economy strategies as major mechanisms for promoting species coexistence in plant assemblages in semiarid Mediterranean habitats. Our findings point to high phylogenetic diversity among neighboring plants as a plausible feature underpinning the coexistence of species, because the success of each species in terms of surviving and producing offspring in drought conditions was greater when the initial phylogenetic diversity was higher. Our study is a step forward to understand how phylogenetic relatedness is connected to the mechanisms determining the maintenance of biodiversity.Natural gas seeps release significant amounts of methane and other gases including ethane and propane contributing to global climate change. In this study, bacterial actively\uffc2\uffa0consuming short-chain alkanes were identified by cultivation, whole-genome sequencing, and stable-isotope probing (SIP)-metagenomics using 13C-propane and 13C-ethane from two different natural gas seeps, Pipe Creek and Andreiasu Everlasting Fire. Nearly 100 metagenome-assembled genomes (MAGs) (completeness 70\uffe2\uff80\uff9399%) were recovered from both sites. Among these, 16 MAGs had genes encoding the soluble di-iron monooxygenase (SDIMO). The MAGs were affiliated to Actinobacteria (two MAGs), Alphaproteobacteria (ten MAGs), and Gammaproteobacteria (four MAGs). Additionally, three gaseous-alkane degraders were isolated in pure culture, all of which could grow on ethane, propane, and butane and possessed SDIMO-related genes. Two Rhodoblastus strains (PC2 and PC3) were from Pipe Creek and a Mycolicibacterium strain (ANDR5) from Andreiasu. Strains PC2 and PC3 encoded putative butane monooxygenases (MOs) and strain ANDR5 contained a propane MO. Mycolicibacterium strain ANDR5 and MAG19a, highly abundant in incubations with 13C-ethane, share an amino acid identity (AAI) of 99.3%. We show using a combination of enrichment and isolation, and cultivation-independent techniques, that these natural gas seeps contain a diverse community of active bacteria oxidising\uffc2\uffa0gaseous-alkanes, which play an important role in biogeochemical cycling of natural gas.
", "keywords": ["0301 basic medicine", "570", "Ethane", "0303 health sciences", "Bacteria", "Natural Gas", "Article", "Mixed Function Oxygenases", "Propane", "03 medical and health sciences", "13. Climate action", "Alkanes", "Butanes", "Gases", "Phylogeny"]}, "links": [{"href": "https://ueaeprints.uea.ac.uk/id/eprint/84325/1/s41396_022_01211_0.pdf"}, {"href": "https://www.nature.com/articles/s41396-022-01211-0.pdf"}, {"href": "https://doi.org/10.1038/s41396-022-01211-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41396-022-01211-0", "name": "item", "description": "10.1038/s41396-022-01211-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41396-022-01211-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-22T00:00:00Z"}}, {"id": "10.1038/s41467-019-10373-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:11Z", "type": "Journal Article", "created": "2019-05-30", "title": "A few Ascomycota taxa dominate soil fungal communities worldwide", "description": "AbstractDespite having key functions in terrestrial ecosystems, information on the dominant soil fungi and their ecological preferences at the global scale is lacking. To fill this knowledge gap, we surveyed 235 soils from across the globe. Our findings indicate that 83 phylotypes (<0.1% of the retrieved fungi), mostly belonging to wind dispersed, generalist Ascomycota, dominate soils globally. We identify patterns and ecological drivers of dominant soil fungal taxa occurrence, and present a map of their distribution in soils worldwide. Whole-genome comparisons with less dominant, generalist fungi point at a significantly higher number of genes related to stress-tolerance and resource uptake in the dominant fungi, suggesting that they might be better in colonising a wide range of environments. Our findings constitute a major advance in our understanding of the ecology of fungi, and have implications for the development of strategies to preserve them and the ecosystem functions they provide.Vitaceae) with amplicon sequence data structures", "description": "Abstract
Impacts of plant genotype on microbial assemblage in the phyllosphere (above-ground parts of plants, which predominantly consists of the set of photosynthetic leaves) of Vitis vinifera cultivars have been studied previously but the impact of grape species (under the grape family Vitaceae) was never investigated. Considering the fact, that the phyllosphere microbiome may have profound effects on host plant health and its performance traits, studying the impact of grape species in microbial taxa structuring in the phyllosphere could be of crucial importance. We performed 16S and ITS profiling (for bacteria and fungi respectively) to access genus level characterization of the microflora present in the leaf phyllosphere of five species within this plant family, sampled in two successive years from the repository situated in the Mediterranean. We also performed \uffce\uffb1 and \uffce\uffb2-diversity analyses with robust statistical estimates to test the impacts of grape species and growing year, over a two-year period. Our results indicated the presence of complex microbial diversity and assemblages in the phyllosphere with a significant effect of both factors (grape species and growing year), the latter effect is being more pronounced. We also compared separate normalization methods for high-throughput microbiome data-sets followed by differential taxa abundance analyses. The results suggested the predominance of a particular normalization method over others. This also indicated the need for more robust normalization methods to study the differential taxa abundance among groups in microbiome research.
", "keywords": ["580", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Vegetal Biology", "Bacteria", "Microbiota", "Fungi", "microbiome", "15. Life on land", "Article", "Plant Leaves", "03 medical and health sciences", "vitis vinifera", "diversit\u00e9 microbienne", "phyllosphere", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "effet g\u00e9notype", "Vitis", "Biologie v\u00e9g\u00e9tale", "Phylogeny"]}, "links": [{"href": "https://www.nature.com/articles/s41598-019-50839-0.pdf"}, {"href": "https://hal.inrae.fr/hal-02617774/file/2019_Singh_Scientific%20Reports_1.pdf"}, {"href": "https://doi.org/10.1038/s41598-019-50839-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-019-50839-0", "name": "item", "description": "10.1038/s41598-019-50839-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-019-50839-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-04T00:00:00Z"}}, {"id": "10.1038/s41598-020-68099-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:17Z", "type": "Journal Article", "created": "2020-07-03", "title": "Growth rate trades off with enzymatic investment in soil filamentous fungi", "description": "AbstractSaprobic soil fungi drive many important ecosystem processes, including decomposition, and many of their effects are related to growth rate and enzymatic ability. In mycology, there has long been the implicit assumption of a trade-off between growth and enzymatic investment, which we test here using a set of filamentous fungi from the same soil. For these fungi we measured growth rate (as colony radial extension) and enzymatic repertoire (activities of four enzymes: laccase, cellobiohydrolase, leucine aminopeptidase and acid phosphatase), and explored the interaction between the traits based on phylogenetically corrected methods. Our results support the existence of a trade-off, however only for the enzymes presumably representing a larger metabolic cost (laccase and cellobiohydrolase). Our study offers new insights into potential functional complementarity within the soil fungal community in ecosystem processes, and experimentally supports an enzymatic investment/growth rate trade-off underpinning phenomena including substrate succession.
", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen", " Pilze", " Algen", "Acid Phosphatase", "Laccase", "Fungi", "579", "15. Life on land", "Article", "Fungal Proteins", "Microbial ecology", "Leucyl Aminopeptidase", "03 medical and health sciences", "Cellulose 1", "4-beta-Cellobiosidase", "Fungal ecology", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/360511v1.full.pdf"}, {"href": "https://www.nature.com/articles/s41598-020-68099-8.pdf"}, {"href": "https://doi.org/10.1038/s41598-020-68099-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-020-68099-8", "name": "item", "description": "10.1038/s41598-020-68099-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-020-68099-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-02T00:00:00Z"}}, {"id": "10.1038/s41598-023-49194-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:17Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "AbstractBacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.The Amazon rainforest is the Earth\uffe2\uff80\uff99s largest reservoir of plant and animal diversity, and it has been subjected to especially high rates of land use change, primarily to cattle pasture. This conversion has had a strongly negative effect on biological diversity, reducing the number of plant and animal species and homogenizing communities. We report here that microbial biodiversity also responds strongly to conversion of the Amazon rainforest, but in a manner different from plants and animals. Local taxonomic and phylogenetic diversity of soil bacteria increases after conversion, but communities become more similar across space. This homogenization is driven by the loss of forest soil bacteria with restricted ranges (endemics) and results in a net loss of diversity. This study shows homogenization of microbial communities in response to human activities. Given that soil microbes represent the majority of biodiversity in terrestrial ecosystems and are intimately involved in ecosystem functions, we argue that microbial biodiversity loss should be taken into account when assessing the impact of land use change in tropical forests.
", "keywords": ["0301 basic medicine", "2. Zero hunger", "Tropical Climate", "0303 health sciences", "Bacteria", "Rain", "Agriculture", "Biodiversity", "15. Life on land", "Trees", "03 medical and health sciences", "13. Climate action", "Animals", "Humans", "Cattle", "Brazil", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1073/pnas.1220608110"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.1220608110", "name": "item", "description": "10.1073/pnas.1220608110", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.1220608110"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-12-27T00:00:00Z"}}, {"id": "10.1073/pnas.1812668115", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:38Z", "type": "Journal Article", "created": "2018-11-30", "title": "Poplar phyllosphere harbors disparate isoprene-degrading bacteria", "description": "The climate-active gas isoprene (2-methyl-1,3-butadiene) is released to the atmosphere in huge quantities, almost equaling that of methane, yet we know little about the biological cycling of isoprene in the environment. Although bacteria capable of growth on isoprene as the sole source of carbon and energy have previously been isolated from soils and sediments, no microbiological studies have targeted the major source of isoprene and examined the phyllosphere of isoprene-emitting trees for the presence of degraders of this abundant carbon source. Here, we identified isoprene-degrading bacteria in poplar tree-derived microcosms by DNA stable isotope probing. The genomes of isoprene-degrading taxa were reconstructed, putative isoprene metabolic genes were identified, and isoprene-related gene transcription was analyzed by shotgun metagenomics and metatranscriptomics. Gram-positive bacteria of the genus Rhodococcus proved to be the dominant isoprene degraders, as previously found in soil. However, a wider diversity of isoprene utilizers was also revealed, notably Variovorax , a genus not previously associated with this trait. This finding was confirmed by expression of the isoprene monooxygenase from Variovorax in a heterologous host. A Variovorax strain that could grow on isoprene as the sole carbon and energy source was isolated. Analysis of its genome confirmed that it contained isoprene metabolic genes with an identical layout and high similarity to those identified by DNA-stable isotope probing and metagenomics. This study provides evidence of a wide diversity of isoprene-degrading bacteria in the isoprene-emitting tree phyllosphere and greatly enhances our understanding of the biodegradation of this important metabolite and climate-active gas.
", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "2. Zero hunger", "570", "0303 health sciences", "QH301 Biology", "Biological Sciences", "15. Life on land", "Mixed Function Oxygenases", "Comamonadaceae", "03 medical and health sciences", "Biodegradation", " Environmental", "Hemiterpenes", "Populus", "13. Climate action", "Butadienes", "Rhodococcus", "Metagenomics", "Genome", " Bacterial", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://ueaeprints.uea.ac.uk/id/eprint/69197/1/Published_manuscript.pdf"}, {"href": "https://pnas.org/doi/pdf/10.1073/pnas.1812668115"}, {"href": "https://repository.essex.ac.uk/23631/1/1812668115.full.pdf"}, {"href": "https://doi.org/10.1073/pnas.1812668115"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.1812668115", "name": "item", "description": "10.1073/pnas.1812668115", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.1812668115"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-11-29T00:00:00Z"}}, {"id": "10.1093/femsle/fnac029", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:18:57Z", "type": "Journal Article", "created": "2022-03-18", "title": "Cultivation of ammonia-oxidising archaea on solid medium", "description": "AbstractAmmonia-oxidising archaea (AOA) are environmentally important microorganisms involved in the biogeochemical cycling of nitrogen. Routine cultivation of AOA is exclusively performed in liquid cultures and reports on their growth on solid medium are scarce. The ability to grow AOA on solid medium would be beneficial for not only the purification of enrichment cultures but also for developing genetic tools. The aim of this study was to develop a reliable method for growing individual colonies from AOA cultures on solid medium. Three phylogenetically distinct AOA strains were tested: \uffe2\uff80\uff98Candidatus Nitrosocosmicus franklandus C13\uffe2\uff80\uff99, Nitrososphaera viennensis EN76 and \uffe2\uff80\uff98Candidatus Nitrosotalea sinensis Nd2\uffe2\uff80\uff99. Of the gelling agents tested, agar and Bacto-agar severely inhibited growth of all three strains. In contrast, both \uffe2\uff80\uff98Ca. N. franklandus C13\uffe2\uff80\uff99 and N. viennensis EN76 tolerated Phytagel\uffe2\uff84\uffa2 while the acidophilic \uffe2\uff80\uff98Ca. N. sinensis Nd2\uffe2\uff80\uff99 was completely inhibited. Based on these observations, we developed a Liquid-Solid (LS) method that involves immobilising cells in Phytagel\uffe2\uff84\uffa2 and overlaying with liquid medium. This approach resulted in the development of visible distinct colonies from \uffe2\uff80\uff98Ca. N. franklandus C13\uffe2\uff80\uff99 and N. viennensis EN76 cultures and lays the groundwork for the genetic manipulation of this group of microorganisms.The complete genome sequence of Rhodococcus sp. WAY2 (WAY2) consists of a circular chromosome, three linear replicons and a small circular plasmid. The linear replicons contain typical actinobacterial invertron-type telomeres with the central CGTXCGC motif. Comparative phylogenetic analysis of the 16S rRNA gene along with phylogenomic analysis based on the genome-to-genome blast distance phylogeny (GBDP) algorithm and digital DNA\uffe2\uff80\uff93DNA hybridization (dDDH) with other Rhodococcus type strains resulted in a clear differentiation of WAY2, which is likely a new species. The genome of WAY2 contains five distinct clusters of bph, etb and nah genes, putatively involved in the degradation of several aromatic compounds. These clusters are distributed throughout the linear plasmids. The high sequence homology of the ring-hydroxylating subunits of these systems with other known enzymes has allowed us to model the range of aromatic substrates they could degrade. Further functional characterization revealed that WAY2 was able to grow with biphenyl, naphthalene and xylene as sole carbon and energy sources, and could oxidize multiple aromatic compounds, including ethylbenzene, phenanthrene, dibenzofuran and toluene. In addition, WAY2 was able to co-metabolize 23 polychlorinated biphenyl congeners, consistent with the five different ring-hydroxylating systems encoded by its genome. WAY2 could also use n-alkanes of various chain-lengths as a sole carbon source, probably due to the presence of alkB and ladA gene copies, which are only found in its chromosome. These results show that WAY2 has a potential to be used for the biodegradation of multiple organic compounds.
", "keywords": ["0301 basic medicine", "0303 health sciences", "PCB", "Whole Genome Sequencing", "AlkB Enzymes", "Rhodococcus; biodegradation; PAH; PCB; hydrocarbons; complete genome", "High-Throughput Nucleotide Sequencing", "PAH", "Naphthalenes", "Xylenes", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "biodegradation", "Polychlorinated Biphenyls", "Hydrocarbons", "Complete genome", "03 medical and health sciences", "Biodegradation", " Environmental", "RNA", " Ribosomal", " 16S", "Biodegradation", "Cluster Analysis", "Rhodococcus", "Phylogeny", "Research Article"]}, "links": [{"href": "https://doi.org/10.1099/mgen.0.000363"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Genomics", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1099/mgen.0.000363", "name": "item", "description": "10.1099/mgen.0.000363", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1099/mgen.0.000363"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-01T00:00:00Z"}}, {"id": "10.1101/117887", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:05Z", "type": "Journal Article", "created": "2017-03-19", "title": "Bacterial physiological adaptations to contrasting edaphic conditions identified using landscape scale metagenomics", "description": "AbstractEnvironmental factors relating to soil pH are widely known to be important in structuring soil bacterial communities, yet the relationship between taxonomic community composition and functional diversity remains to be determined. Here, we analyze geographically distributed soils spanning a wide pH gradient and assess the functional gene capacity within those communities using whole genome metagenomics. Low pH soils consistently had fewer taxa (lower alpha and gamma diversity), but only marginal reductions in functional alpha diversity and equivalent functional gamma diversity. However, coherent changes in the relative abundances of annotated genes between pH classes were identified; with functional profiles clustering according to pH independent of geography. Differences in gene abundances were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-mediated mechanisms for organic C and N substrate acquisition. These findings show that bacterial functional versatility may not be constrained by taxonomy, and we further identify the range of physiological adaptations required to exist in soils of varying nutrient availability and edaphic conditions.Bacteria of Lactobacillus sp. are very useful to humans. However, the biology and genomic diversity of their (bacterio)phage enemies remains understudied. Knowledge on Lactobacillus phage diversity should broaden to develop efficient phage control strategies. To this end, organic waste samples were screened for phages against two wine-related Lactobacillus plantarum strains. Isolates were shotgun sequenced and compared against the phage database and each other by phylogenetics and comparative genomics. The new isolates had only three distant relatives from the database, but displayed a high overall degree of genomic similarity amongst them. The latter allowed for the use of one isolate as a representative to conduct transmission electron microscopy and structural protein sequencing, and to study phage adsorption and growth kinetics. The microscopy and proteomics tests confirmed the observed diversity of the new isolates and supported their classification to the family Siphoviridae and the proposal of the new phage genus \uffe2\uff80\uff9cSilenusvirus\uffe2\uff80\uff9d.
", "keywords": ["0301 basic medicine", "0303 health sciences", "FRAMESHIFT", "Denmark", "BACTERIOPHAGES", "PROTEIN", "Wine", "Genome", " Viral", "Viral Plaque Assay", "SEQUENCE", "CLASSIFICATION", "Article", "12. Responsible consumption", "Microscopy", " Electron", "Waste Disposal Facilities", "03 medical and health sciences", "Bacteriolysis", "Species Specificity", "DNA", " Viral", "Bacteriophages", "Adsorption", "Phylogeny", "Lactobacillus plantarum"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/728261v1.full.pdf"}, {"href": "https://doi.org/10.1101/728261"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1101/728261", "name": "item", "description": "10.1101/728261", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1101/728261"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-07T00:00:00Z"}}, {"id": "10.1111/1462-2920.13956", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:14Z", "type": "Journal Article", "created": "2017-10-13", "title": "Type VI secretion systems in plant-associated bacteria", "description": "SummaryThe type VI secretion system (T6SS) is a bacterial nanomachine used to inject effectors into prokaryotic or eukaryotic cells and is thus involved in both host manipulation and interbacterial competition. The T6SS is widespread among Gram\uffe2\uff80\uff90negative bacteria, mostly within the Proteobacterium Phylum. This secretion system is commonly found in commensal and pathogenic plant\uffe2\uff80\uff90associated bacteria. Phylogenetic analysis of phytobacterial T6SS clusters shows that they are distributed in the five main clades previously described (group 1\uffe2\uff80\uff935). The even distribution of the system among commensal and pathogenic phytobacteria suggests that the T6SS provides fitness and colonization advantages in planta and that the role of the T6SS is not restricted to virulence. This manuscript reviews the phylogeny and biological roles of the T6SS in plant\uffe2\uff80\uff90associated bacteria, highlighting a remarkable diversity both in terms of mechanism and function.
", "keywords": ["PROTEIN SECRETION", "0301 basic medicine", "570", "VIBRIO-CHOLERAE", "PATHOGENIC BACTERIA", "Microbiology", "03 medical and health sciences", "Bacterial Proteins", "Proteobacteria", "Phylogeny", "Plant Diseases", "2. Zero hunger", "0303 health sciences", "Science & Technology", "Virulence", "PSEUDOMONAS-AERUGINOSA", "Minireviews", "Plants", "Type VI Secretion Systems", "IN-SILICO ANALYSIS", "AGROBACTERIUM-TUMEFACIENS", "INTERBACTERIAL COMPETITION", "GENOMIC ANALYSIS", "EFFECTORS", "VIRULENCE", "Life Sciences & Biomedicine", "0605 Microbiology"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13956"}, {"href": "https://doi.org/10.1111/1462-2920.13956"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.13956", "name": "item", "description": "10.1111/1462-2920.13956", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.13956"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-11-10T00:00:00Z"}}, {"id": "10.1111/1462-2920.16316", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:14Z", "type": "Journal Article", "created": "2023-01-04", "title": "The effect of methane and methanol on the terrestrial ammonia\u2010oxidizing archaeon \u2018Candidatus Nitrosocosmicus franklandus C13\u2019", "description": "AbstractThe ammonia monooxygenase (AMO) is a key enzyme in ammonia\uffe2\uff80\uff90oxidizing archaea, which are abundant and ubiquitous in soil environments. The AMO belongs to the copper\uffe2\uff80\uff90containing membrane monooxygenase (CuMMO) enzyme superfamily, which also contains particulate methane monooxygenase (pMMO). Enzymes in the CuMMO superfamily are promiscuous, which results in co\uffe2\uff80\uff90oxidation of alternative substrates. The phylogenetic and structural similarity between the pMMO and the archaeal AMO is well\uffe2\uff80\uff90established, but there is surprisingly little information on the influence of methane and methanol on the archaeal AMO and terrestrial nitrification. The aim of this study was to examine the effects of methane and methanol on the soil ammonia\uffe2\uff80\uff90oxidizing archaeon \uffe2\uff80\uff98Candidatus Nitrosocosmicus franklandus C13\uffe2\uff80\uff99. We demonstrate that both methane and methanol are competitive inhibitors of the archaeal AMO. The inhibition constants (Ki) for methane and methanol were 2.2 and 20\uffe2\uff80\uff89\uffce\uffbcM, respectively, concentrations which are environmentally relevant and orders of magnitude lower than those previously reported for ammonia\uffe2\uff80\uff90oxidizing bacteria. Furthermore, we demonstrate that a specific suite of proteins is upregulated and downregulated in \uffe2\uff80\uff98Ca. Nitrosocosmicus franklandus C13\uffe2\uff80\uff99 in the presence of methane or methanol, which provides a foundation for future studies into metabolism of one\uffe2\uff80\uff90carbon (C1) compounds in ammonia\uffe2\uff80\uff90oxidizing archaea.Anthropogenic nitrogen (N) loading alters soil ammonia\uffe2\uff80\uff90oxidizing archaea (AOA) and bacteria (AOB) abundances, likely leading to substantial changes in soil nitrification. However, the factors and mechanisms determining the responses of soil AOA:AOB and nitrification to N loading are still unclear, making it difficult to predict future changes in soil nitrification. Herein, we synthesize\uffc2\uffa068 field studies around the world to evaluate the impacts of N loading on soil ammonia oxidizers and nitrification. Across a wide range of biotic and abiotic factors, climate is the most important driver of the responses of AOA:AOB to N loading. Climate does not directly affect the N\uffe2\uff80\uff90stimulation of nitrification, but does so via climate\uffe2\uff80\uff90related shifts in AOA:AOB. Specifically, climate modulates the responses of AOA:AOB to N loading by affecting soil pH, N\uffe2\uff80\uff90availability and moisture. AOB play a dominant role in affecting nitrification in dry climates, while the impacts from AOA can exceed AOB in humid climates. Together, these results suggest that climate\uffe2\uff80\uff90related shifts in soil ammonia\uffe2\uff80\uff90oxidizing community maintain the N\uffe2\uff80\uff90stimulation of nitrification, highlighting the importance of microbial community composition in mediating the responses of the soil N cycle to N loading.Many studies have shown that changes in nitrogen (N) availability affect primary productivity in a variety of terrestrial systems, but less is known about the effects of the changing N cycle on soil organic matter (SOM) decomposition. We used a variety of techniques to examine the effects of chronic N amendments on SOM chemistry and microbial community structure and function in an alpine tundra soil. We collected surface soil (0\uffe2\uff80\uff935\uffe2\uff80\uff83cm) samples from five control and five long\uffe2\uff80\uff90term N\uffe2\uff80\uff90amended plots established and maintained at the Niwot Ridge Long\uffe2\uff80\uff90term Ecological Research (LTER) site. Samples were bulked by treatment and all analyses were conducted on composite samples. The fungal community shifted in response to N amendments, with a decrease in the relative abundance of basidiomycetes. Bacterial community composition also shifted in the fertilized soil, with increases in the relative abundance of sequences related to the Bacteroidetes and Gemmatimonadetes, and decreases in the relative abundance of the Verrucomicrobia. We did not uncover any bacterial sequences that were closely related to known nitrifiers in either soil, but sequences related to archaeal nitrifiers were found in control soils. The ratio of fungi to bacteria did not change in the N\uffe2\uff80\uff90amended soils, but the ratio of archaea to bacteria dropped from 20% to less than 1% in the N\uffe2\uff80\uff90amended plots. Comparisons of aliphatic and aromatic carbon compounds, two broad categories of soil carbon compounds, revealed no between treatment differences. However, G\uffe2\uff80\uff90lignins were found in higher relative abundance in the fertilized soils, while proteins were detected in lower relative abundance. Finally, the activities of two soil enzymes involved in N cycling changed in response to chronic N amendments. These results suggest that chronic N fertilization induces significant shifts in soil carbon dynamics that correspond to shifts in microbial community structure and function.
", "keywords": ["2. Zero hunger", "Bacteria", "Nitrogen", "Molecular Sequence Data", "Fungi", "Biodiversity", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "15. Life on land", "Archaea", "Carbon", "6. Clean water", "Soil", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Organic Chemicals", "Fertilizers", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1111/j.1462-2920.2008.01735.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1462-2920.2008.01735.x", "name": "item", "description": "10.1111/j.1462-2920.2008.01735.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1462-2920.2008.01735.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-09-30T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2007.00394.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:43Z", "type": "Journal Article", "created": "2007-10-19", "title": "Quantitation And Diversity Analysis Of Ruminal Methanogenic Populations In Response To The Antimethanogenic Compound Bromochloromethane", "description": "Methyl coenzyme-M reductase A (mcrA) clone libraries were generated from microbial DNA extracted from the rumen of cattle fed a roughage diet with and without supplementation of the antimethanogenic compound bromochloromethane. Bromochloromethane reduced total methane emissions by c. 30%, with a resultant increase in propionate and branched chain fatty acids. The mcrA clone libraries revealed that Methanobrevibacter spp. were the dominant species identified. A decrease in the incidence of Methanobrevibacter spp. from the clone library generated from bromochloromethane treatment was observed. In addition, a more diverse methanogenic population with representatives from Methanococcales, Methanomicrobiales and Methanosacinales orders was observed for the bromochloromethane library. Sequence data generated from these libraries aided in the design of an mcrA-targeted quantitative PCR (qPCR) assay. The reduction in methane production by bromochloromethane was associated with an average decrease of 34% in the number of methanogenic Archaea when monitored with this qPCR assay. Dissociation curve analysis of mcrA amplicons showed a clear difference in melting temperatures for Methanobrevibacter spp. (80-82 degrees C) and all other methanongens (84-86 degrees C). A decrease in the intensity of the Methanobrevibacter spp. specific peak and an increase for the other peak in the bromochloromethane-treated animals corresponded with the changes within the clone libraries.", "keywords": ["Male", "0301 basic medicine", "Rumen", "Bromochloromethane", "Methanogens", "Molecular Sequence Data", "Euryarchaeota", "Methanobrevibacter", "Polymerase Chain Reaction", "630", "03 medical and health sciences", "2402 Applied Microbiology and Biotechnology", "Animals", "Methyl coenzyme-M reductase", "Phylogeny", "Gene Library", "2. Zero hunger", "0303 health sciences", "Hydrocarbons", " Halogenated", "2404 Microbiology", "Sequence Analysis", " DNA", "mcrA", "qPCR", "DNA", " Archaeal", "Cattle", "Oxidoreductases", "2303 Ecology", "Methane"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2007.00394.x", "name": "item", "description": "10.1111/j.1574-6941.2007.00394.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-12-01T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2011.01192.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:43Z", "type": "Journal Article", "created": "2011-09-01", "title": "Soil Characteristics More Strongly Influence Soil Bacterial Communities Than Land-Use Type", "description": "To gain insight into the factors driving the structure of bacterial communities in soil, we applied real-time PCR, PCR-denaturing gradient gel electrophoreses, and phylogenetic microarray approaches targeting the 16S rRNA gene across a range of different land usages in the Netherlands. We observed that the main differences in the bacterial communities were not related to land-use type, but rather to soil factors. An exception was the bacterial community of pine forest soils (PFS), which was clearly different from all other sites. PFS had lowest bacterial abundance, lowest numbers of operational taxonomic units (OTUs), lowest soil pH, and highest C : N ratios. C : N ratio strongly influenced bacterial community structure and was the main factor separating PFS from other fields. For the sites other than PFS, phosphate was the most important factor explaining the differences in bacterial communities across fields. Firmicutes were the most dominant group in almost all fields, except in PFS and deciduous forest soils (DFS). In PFS, Alphaproteobacteria was most represented, while in DFS, Firmicutes and Gammaproteobacteria were both highly represented. Interestingly, Bacillii and Clostridium OTUs correlated with pH and phosphate, which might explain their high abundance across many of the Dutch soils. Numerous bacterial groups were highly correlated with specific soil factors, suggesting that they might be useful as indicators of soil status.", "keywords": ["land use change", "DNA", " Bacterial", "0301 basic medicine", "RNA 16S", "polymerase chain reaction", "soil nitrogen", "DNA sequence", "soil microorganism", "electrokinesis", "chemistry", "phylogeny", "Real-Time Polymerase Chain Reaction", "soil", "Soil", "03 medical and health sciences", "NIOO", "RNA", " Ribosomal", " 16S", "genetics", "soil carbon", "Phylogeny", "Soil Microbiology", "phosphate", "biodiversity", "Alphaproteobacteria", "Netherlands", "growth", " development and aging", "2. Zero hunger", "abundance", "0303 health sciences", "real time", "Bacteria", "pH", "Denaturing Gradient Gel Electrophoresis", "microbiology", "denaturing gradient gel electrophoresis", "Biodiversity", "Sequence Analysis", " DNA", "15. Life on land", "bacterium", "bacterial DNA", "phylogenetics", "classification", "real time polymerase chain reaction", "microbial community", "Gammaproteobacteria"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2011.01192.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2011.01192.x", "name": "item", "description": "10.1111/j.1574-6941.2011.01192.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2011.01192.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-09-19T00:00:00Z"}}, {"id": "10.1111/mec.13010", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:47Z", "type": "Journal Article", "created": "2014-11-20", "title": "Long-Term Balanced Fertilization Increases The Soil Microbial Functional Diversity In A Phosphorus-Limited Paddy Soil", "description": "AbstractThe influence of long\uffe2\uff80\uff90term chemical fertilization on soil microbial communities has been one of the frontier topics of agricultural and environmental sciences and is critical for linking soil microbial flora with soil functions. In this study, 16S rRNA gene pyrosequencing and a functional gene array, geochip 4.0, were used to investigate the shifts in microbial composition and functional gene structure in paddy soils with different fertilization treatments over a 22\uffe2\uff80\uff90year period. These included a control without fertilizers; chemical nitrogen fertilizer (N); N and phosphate (NP); N and potassium (NK); and N, P and K (NPK). Based on 16S rRNA gene data, both species evenness and key genera were affected by P fertilization. Functional gene array\uffe2\uff80\uff90based analysis revealed that long\uffe2\uff80\uff90term fertilization significantly changed the overall microbial functional structures. Chemical fertilization significantly increased the diversity and abundance of most genes involved in C, N, P and S cycling, especially for the treatments NK and NPK. Significant correlations were found among functional gene structure and abundance, related soil enzymatic activities and rice yield, suggesting that a fertilizer\uffe2\uff80\uff90induced shift in the microbial community may accelerate the nutrient turnover in soil, which in turn influenced rice growth. The effect of N fertilization on soil microbial functional genes was mitigated by the addition of P fertilizer in this P\uffe2\uff80\uff90limited paddy soil, suggesting that balanced chemical fertilization is beneficial to the soil microbial community and its functions.
", "keywords": ["2. Zero hunger", "Bacteria", "Nitrogen", "Oryza", "Phosphorus", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "Nitrogen Cycle", "15. Life on land", "6. Clean water", "Carbon Cycle", "Soil", "13. Climate action", "RNA", " Ribosomal", " 16S", "Potassium", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1111/mec.13010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Molecular%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/mec.13010", "name": "item", "description": "10.1111/mec.13010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/mec.13010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-12-31T00:00:00Z"}}, {"id": "10.1111/mec.14358", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-13T16:19:47Z", "type": "Journal Article", "created": "2017-09-19", "title": "Speciation below ground: Tempo and mode of diversification in a radiation of endogean ground beetles", "description": "AbstractDispersal is a critical factor determining the spatial scale of speciation, which is constrained by the ecological characteristics and distribution of a species\uffe2\uff80\uff99 habitat and the intrinsic traits of species. Endogean taxa are strongly affected by the unique qualities of the below\uffe2\uff80\uff90ground environment and its effect on dispersal, and contrasting reports indicate either high dispersal capabilities favoured by small body size and mediated by passive mechanisms, or low dispersal due to restricted movement and confinement inside the soil. We studied a species\uffe2\uff80\uff90rich endogean ground beetle lineage, Typhlocharina, including three genera and more than 60 species, as a model for the evolutionary biology of dispersal and speciation in the deep soil. A time\uffe2\uff80\uff90calibrated molecular phylogeny generated from >400 individuals was used to delimit candidate species, to study the accumulation of lineages through space and time by species\uffe2\uff80\uff93area\uffe2\uff80\uff93age relationships and to determine the geographical structure of the diversification using the relationship between phylogenetic and geographic distances across the phylogeny. Our results indicated a small spatial scale of speciation in Typhlocharina and low dispersal capacity combined with sporadic long distance, presumably passive dispersal events that fuelled the speciation process. Analysis of lineage growth within Typhlocharina revealed a richness plateau correlated with the range of distribution of lineages, suggesting a long\uffe2\uff80\uff90term species richness equilibrium mediated by density dependence through limits of habitat availability. The interplay of area\uffe2\uff80\uff90 and age\uffe2\uff80\uff90dependent processes ruling the lineage diversification in Typhlocharina may serve as a general model for the evolution of high species diversity in endogean mesofauna.