{"type": "FeatureCollection", "features": [{"id": "10.1016/j.envexpbot.2020.104095", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:14Z", "type": "Journal Article", "created": "2020-04-25", "title": "Alternation of wet and dry sides during partial rootzone drying irrigation enhances leaf ethylene evolution", "description": "Soil drying increases endogenous ABA and ACC concentrations in planta, but how these compounds interact to regulate stomatal responses to soil drying and re-watering is still unclear. To determine the temporal dynamics and physiological significance of root, xylem and leaf ABA and ACC concentrations in response to deficit irrigation (DI) or partial rootzone drying (PRD-F) and re-watering, these variables were measured in plants exposed to similar whole pot soil water contents. Both DI and PRD-F plants received only a fraction of the irrigation supplied to well-watered (WW) plants, either to all (DI) or part (PRD-F) of the rootzone of plants grown in split-pots. Both DI and PRD-F induced partial stomatal closure, increased root ABA and ACC accumulation consistent with local soil water content, but did not affect xylem or leaf concentrations of these compounds compared to WW plants. Two hours after re-watering all (DI-RW) or part of the rootzone (PRD-A) to the same soil water content, stomatal conductance returned to WW values or further decreased respectively. Re-watering the whole rootzone had no effect on xylem and leaf ABA and ACC concentrations, while re-watering the dry side of the pot in PRD plants had no effect on xylem and leaf ABA concentrations but increased xylem and leaf ACC concentrations and leaf ethylene evolution. Leaf water potential was similar between all irrigation treatments, with stomatal conductance declining as xylem ABA concentrations and leaf ACC concentrations increased. Prior to re-watering PRD plants, accounting for the spatial differences in soil water uptake best explained variation in xylem ACC concentration suggesting root-to-shoot ACC signalling, but this model did not account for variation in xylem ACC concentration after re-watering the dry side of PRD plants. Thus local (foliar) and long-distance (root-to-shoot) variation in ACC status both seem important in regulating the temporal dynamics of foliar ethylene evolution in plants exposed to PRD.", "keywords": ["0106 biological sciences", "Irrigation", "Stomatal conductance", "Root-to-shoot signalling", "Ethylene", "Physiological significance", "Deficit irrigation", "Plant Science", "Leaf water", "F06 Irrigation", "01 natural sciences", "ACC", "Ecology", " Evolution", " Behavior and Systematics", "580", "2. Zero hunger", "Xylem", "15. Life on land", "F60 Plant physiology and biochemistry", "6. Clean water", "Horticulture", "13. Climate action", "Soil water", "Agronomy and Crop Science", "Soil moisture heterogeneity", "Partial rootzone drying"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/144510/1/Juan_EEB_Manuscript_final.pdf"}, {"href": "https://doi.org/10.1016/j.envexpbot.2020.104095"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20and%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envexpbot.2020.104095", "name": "item", "description": "10.1016/j.envexpbot.2020.104095", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envexpbot.2020.104095"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-01T00:00:00Z"}}, {"id": "10.1007/s00442-007-0750-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:14:59Z", "type": "Journal Article", "created": "2007-05-09", "title": "Mangrove Growth In New Zealand Estuaries: The Role Of Nutrient Enrichment At Sites With Contrasting Rates Of Sedimentation", "description": "Mangrove forest coverage is increasing in the estuaries of the North Island of New Zealand, causing changes in estuarine ecosystem structure and function. Sedimentation and associated nutrient enrichment have been proposed to be factors leading to increases in mangrove cover, but the relative importance of each of these factors is unknown. We conducted a fertilization study in estuaries with different sedimentation histories in order to determine the role of nutrient enrichment in stimulating mangrove growth and forest development. We expected that if mangroves were nutrient-limited, nutrient enrichment would lead to increases in mangrove growth and forest structure and that nutrient enrichment of trees in our site with low sedimentation would give rise to trees and sediments that converged in terms of functional characteristics on control sites in our high sedimentation site. The effects of fertilizing with nitrogen (N) varied among sites and across the intertidal zone, with enhancements in growth, photosynthetic carbon gain, N resorption prior to leaf senescence and the leaf area index of canopies being significantly greater at the high sedimentation sites than at the low sedimentation sites, and in landward dwarf trees compared to seaward fringing trees. Sediment respiration (CO(2) efflux) was higher at the high sedimentation site than at the low one sedimentation site, but it was not significantly affected by fertilization, suggesting that the high sedimentation site supported greater bacterial mineralization of sediment carbon. Nutrient enrichment of the coastal zone has a role in facilitating the expansion of mangroves in estuaries of the North Island of New Zealand, but this effect is secondary to that of sedimentation, which increases habitat area and stimulates growth. In estuaries with high sediment loads, enrichment with N will cause greater mangrove growth and further changes in ecosystem function.", "keywords": ["nutrient resorption efficiency", "Whangapoua", "0106 biological sciences", "Geologic Sediments", "Nitrogen", "Performance", "soil respiration", "01 natural sciences", "Rhizophora-mangle", "C1", "Oxygen Consumption", "Plant-growth", "Herbivory", "Photosynthesis", "Deposition", "Ecosystem", "580", "photosynthesis", "Avicenna marina", "Ecology", "leaf area index", "Plant Stems", "Phosphorus", "Soil respiration", "Limitation", "15. Life on land", "Carbon", "Plant Leaves", "Leaf area index", "770400 Coastal and Estuarine Environment", "Nutrient resorption efficiency", "Waikopua", "Avicennia", "Seasons", "270402 Plant Physiology", "New Zealand"]}, "links": [{"href": "https://doi.org/10.1007/s00442-007-0750-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-007-0750-y", "name": "item", "description": "10.1007/s00442-007-0750-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-007-0750-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-05-10T00:00:00Z"}}, {"id": "10.1007/s11104-023-06301-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:26Z", "type": "Journal Article", "created": "2023-10-04", "title": "Root phenotypes for improved nitrogen capture", "description": "Abstract Background

Suboptimal nitrogen availability is a primary constraint for crop production in low-input agroecosystems, while nitrogen fertilization is a primary contributor to the energy, economic, and environmental costs of crop production in high-input agroecosystems. In this article we consider avenues to develop crops with improved nitrogen capture and reduced requirement for nitrogen fertilizer.

Scope

Intraspecific variation for an array of root phenotypes has been associated with improved nitrogen capture in cereal crops, including architectural phenotypes that colocalize root foraging with nitrogen availability in the soil; anatomical phenotypes that reduce the metabolic costs of soil exploration, improve penetration of hard soil, and exploit the rhizosphere; subcellular phenotypes that reduce the nitrogen requirement of plant tissue; molecular phenotypes exhibiting optimized nitrate uptake kinetics; and rhizosphere phenotypes that optimize associations with the rhizosphere microbiome. For each of these topics we provide examples of root phenotypes which merit attention as potential selection targets for crop improvement. Several cross-cutting issues are addressed including the importance of soil hydrology and impedance, phenotypic plasticity, integrated phenotypes, in silico modeling, and breeding strategies using high throughput phenotyping for co-optimization of multiple phenes.

Conclusions

Substantial phenotypic variation exists in crop germplasm for an array of root phenotypes that improve nitrogen capture. Although this topic merits greater research attention than it currently receives, we have adequate understanding and tools to develop crops with improved nitrogen capture. Root phenotypes are underutilized yet attractive breeding targets for the development of the nitrogen efficient crops urgently needed in global agriculture.

", "keywords": ["2. Zero hunger", "0106 biological sciences", "0301 basic medicine", "Plasticity", "Marschner Review", "Nitrogen", "Physiology", "Nitrogen; Root; Anatomy; Architecture; Soil; Crop breeding; Root phenotyping; Modeling; Rhizosphere; Plasticity; Physiology", "Modeling", "Root phenotyping", "15. Life on land", "01 natural sciences", "Soil", "03 medical and health sciences", "Root", "FOS: Biological sciences", "Architecture", "Rhizosphere", "Crop breeding", "Anatomy", "FOS: Civil engineering"]}, "links": [{"href": "https://doi.org/10.1007/s11104-023-06301-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-023-06301-2", "name": "item", "description": "10.1007/s11104-023-06301-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-023-06301-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-04T00:00:00Z"}}, {"id": "10.1016/j.agee.2022.108124", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:46Z", "type": "Journal Article", "created": "2022-08-18", "title": "Assessing almond response to irrigation and soil management practices using vegetation indexes time-series and plant water status measurements", "description": "Open AccessThis research was funded in the frame of the projects PRECIRIEGO RTC-2017\u20136365-2 financed by Agencia Estatal de Investigaci\u00f3n with European Regional Development Fund co-funds; and the European Union H2020 project SHUI GA 773903. The research was supported also by the CajaMar Caja Rural Contract \u201cEfficient use of water resources under climate change scenarios\u201d. I. Buesa and J.M. Ram\u00edrez-Cuesta acknowledge the postdoctoral financial support received from Juan de la Cierva Spanish Postdoctoral Program (FJC2019\u2013042122-I and IJC2020\u2013043601-I, respectively). Authors acknowledge David Hortelano and Jos\u00e9 Luis Ru\u00edz Garc\u00eda for the help provided in the field measurements acquisition. This work represents a contribution to CSIC Thematic Interdisciplinary Platform PTI TELEDETECT.", "keywords": ["0106 biological sciences", "Soil management", "Almonds", "F06 Irrigation", "01 natural sciences", "12. Responsible consumption", "Vegetation index", "Sentinel 2", "Remote sensing sustainable agriculture", "P33 Soil chemistry and physics", "F40 Plant ecology", "2. Zero hunger", "precision agriculture", "Precision agriculture", "Sustainable agriculture", "Water use efficiency", "Vegetation cover", "F07 Soil cultivation", "04 agricultural and veterinary sciences", "Remote sensing", "15. Life on land", "Tree canopy", "F60 Plant physiology and biochemistry", "6. Clean water", "Water management", "P30 Soil science and management", "P10 Water resources and management", "0401 agriculture", " forestry", " and fisheries", "Remote sensing", " sustainable agriculture", "Sentinel-2"]}, "links": [{"href": "https://www.iris.unict.it/bitstream/20.500.11769/552491/2/Agriculture%2c%20ecosystems%20and%20environment%202022.pdf"}, {"href": "https://doi.org/10.1016/j.agee.2022.108124"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2022.108124", "name": "item", "description": "10.1016/j.agee.2022.108124", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2022.108124"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-01T00:00:00Z"}}, {"id": "10.1016/j.foreco.2008.02.005", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:24Z", "type": "Journal Article", "created": "2008-03-12", "title": "Why Does Rainfall Affect The Trend In Soil Carbon After Converting Pastures To Forests? A Possible Explanation Based On Nitrogen Dynamics", "description": "Abstract When trees are planted onto former pastures, soil carbon stocks typically either remain constant or decrease, with decreases more common in regions with higher rainfall. We conducted a modelling analysis to assess whether those changes in soil carbon, especially the interaction with rainfall, could be understood through consideration of nitrogen balances. The study was based on simulations with the whole-system ecophysiological model CenW which allowed explicit modelling of both carbon and nitrogen pools and their fluxes through plants and soil organic matter. We found that in a modelled coniferous forest without excess water input, total system nitrogen stocks remained similar to pre-forestation values because there were few pathways for nitrogen losses, and without biological nitrogen fixation or fertiliser inputs, gains were restricted to small inputs from atmospheric deposition. However, tree biomass and the litter layer accumulated considerable amounts of nitrogen. This accumulation of nitrogen came at the expense of depleting soil nitrogen stocks. With the change from input of grass litter that is low in lignin to forest litter with higher lignin concentration, organic-matter C:N ratios increased so that more carbon could be stored per unit of soil nitrogen which partly negated the effect of reduced nitrogen stocks. The increase in C:N ratios was initially confined to the surface litter layer because of slow transfer of material to the mineral soil. Over a period of decades, soil C:N ratios eventually increased in the soil as well. Simulations with different amounts of precipitation showed that greater amounts of nitrogen were leached from systems where water supply exceeded the plants\u2019 requirements. Reduced nitrogen stocks then caused a subsequent reduction in soil organic carbon stocks. These simulations thus provided a consistent explanation for the observation of greater losses of soil organic carbon in high-rainfall systems after converting pastures to forests. More generally, the simulations showed that explicit modelling of the nitrogen cycle can put important constraints on possible changes in soil-carbon stocks that may occur after land-use change.", "keywords": ["land use change", "Rainfall", "Mitigation", "ecophysiology", "nitrogen cyc Afforestation", "Greenhouse", "Nitrogen", "Rain", "CenW", "Land-use change", "lignin", "Greenhouse effect", "afforestation", "carbon cycle", "Forest", "Reforestation", "Keywords: Carbon", "2. Zero hunger", "atmospheric deposition", "Nitrogen dynamics", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "13. Climate action", "Land use", "ecological modeling", "0401 agriculture", " forestry", " and fisheries", "grassland"], "contacts": [{"organization": "Roger M. Gifford, Miko U. F. Kirschbaum, Miko U. F. Kirschbaum, Lan Bin Guo,", "roles": ["creator"]}]}, "links": [{"href": "https://openresearch-repository.anu.edu.au/bitstream/1885/61047/5/Kirschbaum_Rainfall_affect_in_soil_carbon.pdf.jpg"}, {"href": "https://openresearch-repository.anu.edu.au/bitstream/1885/61047/7/01_Kirschbaum_Why_does_rainfall_affect_the_2008.pdf.jpg"}, {"href": "https://doi.org/10.1016/j.foreco.2008.02.005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forest%20Ecology%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.foreco.2008.02.005", "name": "item", "description": "10.1016/j.foreco.2008.02.005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.foreco.2008.02.005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-04-01T00:00:00Z"}}, {"id": "10.1007/s11270-016-2884-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:27Z", "type": "Journal Article", "created": "2016-05-24", "title": "Root Production Of Fagus Crenata Blume Saplings Grown In Two Soils And Exposed To Elevated Co2 Concentration: An 11-Year Free-Air-Co2 Enrichment (Face) Experiment In Northern Japan", "description": "We examined the root production of a set of Fagus crenata (Siebold\u2019s beech) saplings grown in an infertile immature volcanic ash soil (VA) and another set in a fertile brown forest soil (BF) with both sets exposed to elevated CO2. After the saplings had been exposed to ambient (370\u2013390\u00a0\u03bcmol\u00a0mol\u22121) or elevated (500\u00a0\u03bcmol\u00a0mol\u22121) CO2, during the daytime, for 11 growing seasons, the root systems were excavated. Elevated CO2 boosted the total root production of saplings grown in VA and abolished the negative effect of VA under ambient CO2, but there was no significant effect of elevated CO2 on saplings grown in BF. These results indicate the projected elevated CO2 concentrations may have a different impact in regions with different soil fertility while in regions with VA, a higher net primary production is expected. In addition, we observed large elevated CO2-induced fine-root production and extensive foraging strategy of saplings in both soils, a phenomenon that may partly (a) adjust the biogeochemical cycles of ecosystems, (b) form their response to global change, and (c) increase the size and/or biodiversity of soil fauna. We recommend that future researches consider testing a soil with a higher degree of infertility than the one we tested.", "keywords": ["2. Zero hunger", "0106 biological sciences", "NPP", "Ecophysiology", "Air pollution", "Climate change", "Atmospheric environment", "15. Life on land", "Greenhouse gas", "01 natural sciences", "653"]}, "links": [{"href": "https://doi.org/10.1007/s11270-016-2884-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%2C%20Air%2C%20%26amp%3B%20Soil%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11270-016-2884-1", "name": "item", "description": "10.1007/s11270-016-2884-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11270-016-2884-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-05-23T00:00:00Z"}}, {"id": "10.1016/j.agrformet.2017.10.013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:47Z", "type": "Journal Article", "created": "2017-10-19", "title": "Climate-Smart Agroforestry: Faidherbia Albida Trees Buffer Wheat Against Climatic Extremes In The Central Rift Valley Of Ethiopia", "description": "Abstract Faidherbia albida parklands cover a large area of the Sudano-Sahelian zone of Africa, a region that suffers from soil fertility decline, food insecurity and climate change. The parklands deliver multiple benefits, including fuelwood, soil nutrient replenishment, moisture conservation, and improved crop yield underneath the canopy. Its microclimate modification may provide an affordable climate adaptation strategy which needs to be explored. We carried out an on-farm experiment for three consecutive seasons in the Ethiopian Central Rift Valley with treatments of Faidherbia trees with bare soil underneath, wheat grown beneath Faidherbia and wheat grown in open fields. We tested the sensitivity of wheat yield to tree-mediated variables of photosynthetically active radiation (PAR), air temperature and soil nitrogen, using APSIM-wheat model. Results showed that soil moisture in the sub-soil was the least for wheat with tree, intermediate for sole tree and the highest for open field. Presence of trees resulted in 35\u201355% larger available N close to tree crowns compared with sole wheat. Trees significantly reduced PAR reaching the canopy of wheat growing underneath to optimum levels. Midday air temperature was about 6\u00a0\u00b0C less under the trees than in the open fields. LAI, number of grains spike\u22121, plant height, total aboveground biomass and wheat grain yield were all significantly higher (P", "keywords": ["Crop physiology", "2. Zero hunger", "Competition", "13. Climate action", "Local adaptation", "Climate change", "0401 agriculture", " forestry", " and fisheries", "Facilitation", "04 agricultural and veterinary sciences", "15. Life on land", "Heat stress", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1016/j.agrformet.2017.10.013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agricultural%20and%20Forest%20Meteorology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agrformet.2017.10.013", "name": "item", "description": "10.1016/j.agrformet.2017.10.013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agrformet.2017.10.013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-01T00:00:00Z"}}, {"id": "10.1016/j.jafr.2023.100732", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:16:37Z", "type": "Journal Article", "created": "2023-08-07", "title": "Aeromycological studies in the crops of the main cereals: A systematic review", "description": "Open AccessLes \u00e9tudes a\u00e9romycologiques sur les cultures c\u00e9r\u00e9ali\u00e8res permettent de d\u00e9terminer la variation temporelle des agents pathog\u00e8nes des plantes affectant la culture et de d\u00e9terminer le moment appropri\u00e9 pour appliquer les fongicides. Cependant, ce sujet n'a pas \u00e9t\u00e9 syst\u00e9matiquement revu. L'objectif de ce travail \u00e9tait d'analyser syst\u00e9matiquement toutes les \u00e9tudes a\u00e9romycologiques r\u00e9alis\u00e9es sur le ma\u00efs, le bl\u00e9, le riz, l'avoine, l'orge, le seigle, le sorgho et le millet. Une recherche syst\u00e9matique a \u00e9t\u00e9 effectu\u00e9e dans Scopus depuis le d\u00e9but de la base de donn\u00e9es jusqu'au 1er ao\u00fbt 2022. Les crit\u00e8res d'inclusion \u00e9taient qu'il s'agissait d'\u00e9tudes a\u00e9romycologiques sur le bl\u00e9 ou le riz ou le ma\u00efs ou l'avoine ou le sorgho ou le seigle ou l'orge ou le millet et d'\u00e9tudes publi\u00e9es dans des revues \u00e0 comit\u00e9 de lecture index\u00e9es dans Journal Citation Reports et r\u00e9dig\u00e9es en anglais ou en espagnol. Quarante-trois \u00e9tudes (21 sur le bl\u00e9, 15 sur le riz, 5 sur le ma\u00efs, 1 sur le sorgho et 2 sur l'orge) r\u00e9pondant \u00e0 tous les crit\u00e8res d'\u00e9ligibilit\u00e9 ont \u00e9t\u00e9 incluses (une des \u00e9tudes sur le ma\u00efs a \u00e9galement \u00e9t\u00e9 men\u00e9e sur le bl\u00e9). Aucune \u00e9tude a\u00e9romycologique n'a \u00e9t\u00e9 trouv\u00e9e chez l'avoine, le seigle et le millet. Il a \u00e9t\u00e9 not\u00e9 que la plupart des recherches a\u00e9romycologiques ont \u00e9t\u00e9 men\u00e9es sur les cultures de bl\u00e9 et principalement dans les pays des Am\u00e9riques. De plus, les propagules fongiques sont principalement collect\u00e9es par des m\u00e9thodes non viables, en utilisant divers types de collecteurs. En g\u00e9n\u00e9ral, les \u00e9tudes visaient \u00e0 identifier un agent pathog\u00e8ne sp\u00e9cifique et non \u00e0 la diversit\u00e9 des agents pathog\u00e8nes qui peuvent \u00eatre trouv\u00e9s. La relation des champignons identifi\u00e9s avec les param\u00e8tres m\u00e9t\u00e9orologiques \u00e9tait variable dans les diff\u00e9rentes \u00e9tudes. Cette revue syst\u00e9matique permet de r\u00e9sumer les \u00e9tudes a\u00e9romycologiques qui ont \u00e9t\u00e9 men\u00e9es sur les cultures de bl\u00e9, de riz, de ma\u00efs, de sorgho et d'orge. Il sugg\u00e8re \u00e9galement o\u00f9 les futures \u00e9tudes dans ce domaine devraient \u00eatre dirig\u00e9es, en fonction des limites rencontr\u00e9es.", "keywords": ["Impacts of Elevated CO2 and Ozone on Plant Physiology", "Agriculture (General)", "Health", " Toxicology and Mutagenesis", "Plant Science", "Crop", "S1-972", "Agricultural and Biological Sciences", "Barley", "Biochemistry", " Genetics and Molecular Biology", "TX341-641", "10. No inequality", "Biology", "Sorghum", "2. Zero hunger", "Corn", "Airborne spores", "Nutrition. Foods and food supply", "Life Sciences", "Phylogenetic Analysis", "Cell Biology", "15. Life on land", "2414.06 Hongos", "Agronomy", "3. Good health", "Wheat", "Environmental Science", "Physical Sciences", "Rice", "Indoor Air Quality and Health Effects", "Diversity and Evolution of Fungal Pathogens", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1016/j.jafr.2023.100732"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Agriculture%20and%20Food%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jafr.2023.100732", "name": "item", "description": "10.1016/j.jafr.2023.100732", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jafr.2023.100732"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1016/j.envexpbot.2011.04.011", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:16:14Z", "type": "Journal Article", "created": "2011-04-25", "title": "Experimental Warming Had Little Effect On Carbon-Based Secondary Compounds, Carbon And Nitrogen In Selected Alpine Plants And Lichens", "description": "Accepted version of an article published in the journal: Environmental and Experimental Botany. Published version available on Science Direct: http://dx.doi.org/10.1016/j.envexpbot.2011.04.011", "keywords": ["VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecology: 488", "VDP::Mathematics and natural science: 400::Zoology and botany: 480::Plant physiology: 492", "13. Climate action", "15. Life on land"], "contacts": [{"organization": "Kari Klanderud, Kari Klanderud, Sylvi M. Sandvik, Line Nybakken, Line Nybakken,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.envexpbot.2011.04.011"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20and%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envexpbot.2011.04.011", "name": "item", "description": "10.1016/j.envexpbot.2011.04.011", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envexpbot.2011.04.011"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-10-01T00:00:00Z"}}, {"id": "10.1016/j.isci.2019.10.043", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:16:36Z", "type": "Journal Article", "created": "2019-10-25", "title": "Genetic Loci Associated with Early Salt Stress Responses of Roots", "description": "Salinity is a devastating abiotic stress accounting for major crop losses yearly. Plant roots can strikingly grow away from high-salt patches. This response is termed halotropism and occurs through auxin redistribution in roots in response to a salt gradient. Here, a natural variation screen for the early and NaCl-specific halotropic response of 333 Arabidopsis accessions revealed quantitative differences in the first 24 h. These data were successfully used to identify genetic components associated with the response through Genome-Wide Association Study (GWAS). Follow-up characterization of knockout mutants in Col-0 background confirmed the role of transcription factor WRKY25, cation-proton exchanger CHX13, and a gene of unknown function DOB1 (Double Bending 1) in halotropism. In chx13 and dob1 mutants, ion accumulation and shoot biomass under salt stress were also affected. Thus, our GWAS has identified genetic components contributing to main root halotropism that provide insight into the genetic architecture underlying plant salt responses.", "keywords": ["580", "0301 basic medicine", "2. Zero hunger", "570", "0303 health sciences", "Science", "Q", "Plant Biology", "Biological Sciences", "15. Life on land", "Plant Genetics", "Article", "03 medical and health sciences", "Plant Physiology"]}, "links": [{"href": "https://doi.org/10.1016/j.isci.2019.10.043"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/iScience", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.isci.2019.10.043", "name": "item", "description": "10.1016/j.isci.2019.10.043", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.isci.2019.10.043"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-01T00:00:00Z"}}, {"id": "10.1016/j.jplph.2012.02.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:44Z", "type": "Journal Article", "created": "2012-04-24", "title": "Drought Stress Has Contrasting Effects On Antioxidant Enzymes Activity And Phenylpropanoid Biosynthesis In Fraxinus Ornus Leaves: An Excess Light Stress Affair?", "description": "The experiment was conducted using Fraxinus ornus plants grown outside under full sunlight irradiance, and supplied with 100% (well-watered, WW), 40% (mild drought, MD), or 20% (severe drought, SD) of the daily evapotranspiration demand, with the main objective of exploring the effect of excess light stress on the activity of antioxidant enzymes and phenylpropanoid biosynthesis. Net CO\u2082 assimilation rate at saturating light and daily assimilated CO\u2082 were significantly smaller in SD than in WW and MD plants. Xanthophyll-cycle pigments supported nonphotochemical quenching to a significantly greater extent in SD than in MD and WW leaves. As a consequence, the actual efficiency of PSII (\u03a6(PSII)) was smaller, while the excess excitation-energy in the photosynthetic apparatus was greater in SD than in WW or MD plants. The concentrations of violaxanthin-cycle pigments relative to total chlorophyll (Chl(tot)) exceeded 200 mmol mol\u207b\u00b9 Chl(tot) in SD leaves at the end of the experiment. This leads to hypothesize for zeaxanthin a role not only as nonphotochemical quencher, but also as chloroplast antioxidant. Reductions in ascorbate peroxidase and catalase activities, as drought-stress progressed, were paralleled by greater accumulations of esculetin and quercetin 3-O-glycosides, both phenylpropanoids having effective capacity to scavenge H\u2082O\u2082. The drought-induced accumulation of esculetin and quercetin 3-O-glycosides in the vacuoles of mesophyll cells is consistent with their putative functions as reducing agents for H\u2082O\u2082 in excess light-stressed leaves. Nonetheless, the concentration of H\u2082O\u2082 and the lipid peroxidation were significantly greater in SD than in MD and WW leaves. It is speculated that vacuolar phenylpropanoids may constitute a secondary antioxidant system, even on a temporal basis, activated upon the depletion of primary antioxidant defences, and aimed at keeping whole-cell H\u2082O\u2082 within a sub-lethal concentration range.", "keywords": ["0301 basic medicine", "Analysis of Variance", "Principal Component Analysis", "0303 health sciences", "Time Factors", "Light", "Propanols", "Antioxidant enzymes Drought stress Phenylpropanoids Water relations Violaxanthin-cycle pigments", "Hydrogen Peroxide", "Pigments", " Biological", "Carbon Dioxide", "15. Life on land", "Antioxidants", "6. Clean water", "Antioxidant enzymes; Drought stress; Phenylpropanoids; Violaxanthin-cycle pigments; Water relations; Analysis of Variance; Antioxidants; Carbon Dioxide; Fraxinus; Hydrogen Peroxide; Malondialdehyde; Mesophyll Cells; Microscopy", " Fluorescence; Photosynthesis; Pigments", " Biological; Plant Leaves; Principal Component Analysis; Propanols; Stress", " Physiological; Time Factors; Droughts; Light; Plant Science; Physiology; Agronomy and Crop Science", "Droughts", "Plant Leaves", "03 medical and health sciences", "Fraxinus", "Microscopy", " Fluorescence", "Stress", " Physiological", "Antioxidant enzymes; drought stress; flavonoids", "Malondialdehyde", "Photosynthesis", "Mesophyll Cells"]}, "links": [{"href": "https://doi.org/10.1016/j.jplph.2012.02.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jplph.2012.02.014", "name": "item", "description": "10.1016/j.jplph.2012.02.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jplph.2012.02.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-07-01T00:00:00Z"}}, {"id": "10.1038/s41396-020-00750-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:43Z", "type": "Journal Article", "created": "2020-10-06", "title": "Acidobacteria are active and abundant members of diverse atmospheric H2-oxidizing communities detected in temperate soils", "description": "Abstract

Significant rates of atmospheric dihydrogen (H2) consumption have been observed in temperate soils due to the activity of high-affinity enzymes, such as the group 1h [NiFe]-hydrogenase. We designed broadly inclusive primers targeting the large subunit gene (hhyL) of group 1h [NiFe]-hydrogenases for long-read sequencing to explore its taxonomic distribution across soils. This approach revealed a diverse collection of microorganisms harboring hhyL, including previously unknown groups and taxonomically not assignable sequences. Acidobacterial group 1h [NiFe]-hydrogenase genes were abundant and expressed in temperate soils. To support the participation of acidobacteria in H2 consumption, we studied two representative mesophilic soil acidobacteria, which expressed group 1h [NiFe]-hydrogenases and consumed atmospheric H2 during carbon starvation. This is the first time mesophilic acidobacteria, which are abundant in ubiquitous temperate soils, have been shown to oxidize H2 down to below atmospheric concentrations. As this physiology allows bacteria to survive periods of carbon starvation, it could explain the success of soil acidobacteria. With our long-read sequencing approach of group 1h [NiFe]-hydrogenase genes, we show that the ability to oxidize atmospheric levels of H2 is more widely distributed among soil bacteria than previously recognized and could represent a common mechanism enabling bacteria to persist during periods of carbon deprivation.Suboptimal nitrogen availability is a primary constraint for crop production in low-input agroecosystems, while nitrogen fertilization is a primary contributor to the energy, economic, and environmental costs of crop production in high-input agroecosystems. In this article we consider avenues to develop crops with improved nitrogen capture and reduced requirement for nitrogen fertilizer.

Scope

Intraspecific variation for an array of root phenotypes has been associated with improved nitrogen capture in cereal crops, including architectural phenotypes that colocalize root foraging with nitrogen availability in the soil; anatomical phenotypes that reduce the metabolic costs of soil exploration, improve penetration of hard soil, and exploit the rhizosphere; subcellular phenotypes that reduce the nitrogen requirement of plant tissue; molecular phenotypes exhibiting optimized nitrate uptake kinetics; and rhizosphere phenotypes that optimize associations with the rhizosphere microbiome. For each of these topics we provide examples of root phenotypes which merit attention as potential selection targets for crop improvement. Several cross-cutting issues are addressed including the importance of soil hydrology and impedance, phenotypic plasticity, integrated phenotypes, in silico modeling, and breeding strategies using high throughput phenotyping for co-optimization of multiple phenes.

Conclusions

Substantial phenotypic variation exists in crop germplasm for an array of root phenotypes that improve nitrogen capture. Although this topic merits greater research attention than it currently receives, we have adequate understanding and tools to develop crops with improved nitrogen capture. Root phenotypes are underutilized yet attractive breeding targets for the development of the nitrogen efficient crops urgently needed in global agriculture.

", "keywords": ["2. Zero hunger", "0106 biological sciences", "0301 basic medicine", "Plasticity", "Marschner Review", "Nitrogen", "Physiology", "Nitrogen; Root; Anatomy; Architecture; Soil; Crop breeding; Root phenotyping; Modeling; Rhizosphere; Plasticity; Physiology", "Modeling", "Root phenotyping", "15. Life on land", "01 natural sciences", "Soil", "03 medical and health sciences", "Root", "FOS: Biological sciences", "Architecture", "Rhizosphere", "Crop breeding", "Anatomy", "FOS: Civil engineering"]}, "links": [{"href": "https://doi.org/20.500.11850/636573"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.11850/636573", "name": "item", "description": "20.500.11850/636573", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.11850/636573"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-04T00:00:00Z"}}, {"id": "10.1038/nature02403", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:41Z", "type": "Journal Article", "created": "2004-04-21", "title": "The Worldwide Leaf Economics Spectrum", "description": "Bringing together leaf trait data spanning 2,548 species and 175 sites we describe, for the first time at global scale, a universal spectrum of leaf economics consisting of key chemical, structural and physiological properties. The spectrum runs from quick to slow return on investments of nutrients and dry mass in leaves, and operates largely independently of growth form, plant functional type or biome. Categories along the spectrum would, in general, describe leaf economic variation at the global scale better than plant functional types, because functional types overlap substantially in their leaf traits. Overall, modulation of leaf traits and trait relationships by climate is surprisingly modest, although some striking and significant patterns can be seen. Reliable quantification of the leaf economics spectrum and its interaction with climate will prove valuable for modelling nutrient fluxes and vegetation boundaries under changing land-use and climate.", "keywords": ["leaf traits", "0106 biological sciences", "INVESTMENT", "Climate", "Rain", "CLIMATE CHANGE", "SEED PRODUCTION", "01 natural sciences", "spectrum", "BIOMASS", "dry mass", "Biologie/Milieukunde (BIOL)", "MODELS", " BIOLOGICAL", "CLIMATE EFFECT", "Nutritional Physiological Phenomena", "Biomass", "Photosynthesis", "LAND USE", "PRIORITY JOURNAL", "functional-groups", "biodiversity", "ALLOMETRY", "2. Zero hunger", "INVESTMENTS", "Geography", "BIOME", "HUMAN", "04 agricultural and veterinary sciences", "INFORMATION SCIENCE", "Life sciences", "tree", "NUTRITION PHYSIOLOGY", "leaf economics", "LEAF ECONOMICS SPECTRUM", "leaves", "ECONOMIC AND SOCIAL EFFECTS", "GEOGRAPHY", "Models", " Biological", "photosynthesis-nitrogen relations", "LEAF", "PLANT LEAF", "nutrients", "high-rainfall", "DATA REDUCTION", "NONHUMAN", "PLANT LEAVES", "NUTRIENT AVAILABILITY", "ARTICLE", "PHYSIOLOGY", "Ecosystem", "580", "life-span", "ECONOMICS", "PHOTOSYNTHESIS", "RAIN", "nutrient", "land use", "area", "use efficiency", "15. Life on land", "PLANT GROWTH", "CLIMATE", "Plant Leaves", "SPECTRUM ANALYSIS", "DRY MASS", "ECOSYSTEM", "0401 agriculture", " forestry", " and fisheries", "BIODIVERSITY", "VEGETATION"]}, "links": [{"href": "https://doi.org/10.1038/nature02403"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/nature02403", "name": "item", "description": "10.1038/nature02403", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/nature02403"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-04-01T00:00:00Z"}}, {"id": "10.1029/2021jg006593", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:17:38Z", "type": "Journal Article", "created": "2021-12-07", "title": "Identifying Data Needed to Reduce Parameter Uncertainty in a Coupled Microbial Soil C and N Decomposition Model", "description": "Abstract

Advancements in microbially explicit ecosystem models incorporate increasingly accurate representations of microbial physiology and enzyme\uffe2\uff80\uff90mediated depolymerization of soil organic matter in predicting biogeochemical responses to global change. However, a major challenge with model structural improvements is the requirement for additional parameters, which are often poorly constrained sources of uncertainty. Furthermore, it is often unclear how to best focus data collection efforts toward reducing model uncertainty. Here, we use Dual Arrhenius Michaelis\uffe2\uff80\uff90Menten Microbial Carbon and Nitrogen Physiology, a microbially mediated, coupled soil C and N cycling model, as a tool to explore the influence of microbial physiological and enzyme kinetic parameters on model estimates. We first quantify the potential for constraining model parameters using empirical measurements of soil respiration. We then use simulated data to identify which additional sources of data collection from the field would provide the greatest impact for constraining model estimates. We find that modeled soil C and N pools and fluxes are disproportionately sensitive to only a few parameters (e.g., activation energies and microbial CUE), while others exert less influence (e.g., Michaelis\uffe2\uff80\uff90Menten half\uffe2\uff80\uff90saturation constants). While some parameters can be constrained by the available data on heterotrophic respiration, the collection of additional data on dissolved organic C and N pools in the soil is identified as a high\uffe2\uff80\uff90priority data need. Improving our ability to model the interactions of soil microbial physiology, soil chemistry, enzyme activities, and environmental factors on C and N cycling will require closely considering model uncertainties and prioritizing future data collection opportunities based on their impact on model performance.

Several studies have investigated soil microbial biodiversity, but understanding of the mechanisms underlying plant responses to soil microbiota remains in its infancy. Here, we focused on tomato (Solanum lycopersicum), testing the hypothesis that plants grown on native soils display different responses to soil microbiotas.

Using transcriptomics, proteomics, and biochemistry, we describe the responses of two tomato genotypes (susceptible or resistant to Fusarium oxysporum f. sp. lycopersici) grown on an artificial growth substrate and two native soils (conducive and suppressive to Fusarium).

Native soils affected tomato responses by modulating pathways involved in responses to oxidative stress, phenol biosynthesis, lignin deposition, and innate immunity, particularly in the suppressive soil. In tomato plants grown on steam\uffe2\uff80\uff90disinfected soils, total phenols and lignin decreased significantly. The inoculation of a mycorrhizal fungus partly rescued this response locally and systemically. Plants inoculated with the fungal pathogen showed reduced disease symptoms in the resistant genotype in both soils, but the susceptible genotype was partially protected from the pathogen only when grown on the suppressive soil.

The \uffe2\uff80\uff98state of alert\uffe2\uff80\uff99 detected in tomatoes reveals novel mechanisms operating in plants in native soils and the soil microbiota appears to be one of the drivers of these plant responses.

", "keywords": ["0301 basic medicine", "Proteome", "Propanols", "Arbuscular mycorrhizal fungi", "arbuscular mycorrhizal fungi", "tomato", "Lignin", "Models", " Biological", "Plant Roots", "defence responses", "Tomato", "Soil", "03 medical and health sciences", "Solanum lycopersicum", "Gene Expression Regulation", " Plant", "Stress", " Physiological", "microbiota", "Plant Immunity", "Soil Microbiology", "suppressive and conducive soils", "susceptible and resistant genotypes", "2. Zero hunger", "0303 health sciences", "Defence responses", "Microbiota", "15. Life on land", "Lignin biosynthesis", "Gene Ontology", "Susceptible and resistant genotypes", "Arbuscular mycorrhizal fungi; Defence responses; Lignin biosynthesis; Microbiota; Suppressive and conducive soils; Susceptible and resistant genotypes; Tomato; Physiology; Plant Science", "Suppressive and conducive soils", "Transcriptome", "lignin biosynthesis"]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/1660820/1/Chialva%20et%20al%20Iris.pdf"}, {"href": "https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.15014"}, {"href": "https://doi.org/10.1111/nph.15014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.15014", "name": "item", "description": "10.1111/nph.15014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.15014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-09T00:00:00Z"}}, {"id": "10.1111/ejss.70078", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:18:37Z", "type": "Journal Article", "created": "2025-03-11", "title": "The Effect of Crop Diversification and Season on Microbial Carbon Use Efficiency Across a European Pedoclimatic Gradient", "description": "ABSTRACT

Microbial transformation of soil organic matter plays a critical role in carbon (C) cycling making it essential to understand how land use and management practices influence microbial physiology and its connection to C dynamics. One factor that is likely to impact soil microbial physiology is crop diversification via its influence on belowground diversity (e.g., chemical heterogeneity of C inputs, microbial community composition). However, the effect of crop diversification measures on microbial physiology and potential effects on C cycling in agricultural soils is still unclear. To address this knowledge gap, we sampled topsoil from eight experimental sites covering different crop diversification measures across Europe (i.e., cover crops, ley farming, vegetation stripes). We used the 18O\uffe2\uff80\uff90labelling method to analyse microbial C use efficiency (CUE), growth, respiration and biomass C. Additionally, a second sampling at five selected sites examined whether the growing season influenced the impact of crop diversification. Meta\uffe2\uff80\uff90analysis revealed no overall effect of crop diversification on CUE, microbial activity, biomass or soil organic C (SOC). However, the effects varied with the type of diversification measure: cover crops did not affect carbon processing, vegetation stripes increased microbial activity, and ley farming enhanced CUE. The largest variation in CUE was observed between samplings at the same sites, indicating seasonal dynamics. Temperature, precipitation and photosynthetically active radiation predicted seasonal variation in CUE (R2\uffe2\uff80\uff89=\uffe2\uff80\uff890.36). While cover crops did not significantly impact C storage in our study, both ley farming and vegetation stripes increased SOC. The overall effect of crop diversification on SOC seems to be decoupled from highly temporally variable CUE in the bulk soil and rather relate to C\uffe2\uff80\uff90inputs.Advancements in microbially explicit ecosystem models incorporate increasingly accurate representations of microbial physiology and enzyme\uffe2\uff80\uff90mediated depolymerization of soil organic matter in predicting biogeochemical responses to global change. However, a major challenge with model structural improvements is the requirement for additional parameters, which are often poorly constrained sources of uncertainty. Furthermore, it is often unclear how to best focus data collection efforts toward reducing model uncertainty. Here, we use Dual Arrhenius Michaelis\uffe2\uff80\uff90Menten Microbial Carbon and Nitrogen Physiology, a microbially mediated, coupled soil C and N cycling model, as a tool to explore the influence of microbial physiological and enzyme kinetic parameters on model estimates. We first quantify the potential for constraining model parameters using empirical measurements of soil respiration. We then use simulated data to identify which additional sources of data collection from the field would provide the greatest impact for constraining model estimates. We find that modeled soil C and N pools and fluxes are disproportionately sensitive to only a few parameters (e.g., activation energies and microbial CUE), while others exert less influence (e.g., Michaelis\uffe2\uff80\uff90Menten half\uffe2\uff80\uff90saturation constants). While some parameters can be constrained by the available data on heterotrophic respiration, the collection of additional data on dissolved organic C and N pools in the soil is identified as a high\uffe2\uff80\uff90priority data need. Improving our ability to model the interactions of soil microbial physiology, soil chemistry, enzyme activities, and environmental factors on C and N cycling will require closely considering model uncertainties and prioritizing future data collection opportunities based on their impact on model performance.Nitrification, the oxidation of ammonia to nitrate, is an essential process in the biogeochemical nitrogen cycle. The first step of nitrification, ammonia oxidation, is performed by three, often co-occurring guilds of chemolithoautotrophs: ammonia-oxidizing bacteria (AOB), archaea (AOA), and complete ammonia oxidizers (comammox). Substrate kinetics are considered to be a major niche-differentiating factor between these guilds, but few AOA strains have been kinetically characterized. Here, the ammonia oxidation kinetic properties of 12 AOA representing all major cultivated phylogenetic lineages were determined using microrespirometry. Members of the genus Nitrosocosmicus have the lowest affinity for both ammonia and total ammonium of any characterized AOA, and these values are similar to previously determined ammonia and total ammonium affinities of AOB. This contrasts previous assumptions that all AOA possess much higher substrate affinities than their comammox or AOB counterparts. The substrate affinity of ammonia oxidizers correlated with their cell surface area to volume ratios. In addition, kinetic measurements across a range of pH values supports the hypothesis that\uffe2\uff80\uff94like for AOB\uffe2\uff80\uff94ammonia and not ammonium is the substrate for the ammonia monooxygenase enzyme of AOA and comammox. Together, these data will facilitate predictions and interpretation of ammonia oxidizer community structures and provide a robust basis for establishing testable hypotheses on competition between AOB, AOA, and comammox.Soil microorganisms act as gatekeepers for soil\uffe2\uff80\uff93atmosphere carbon exchange by balancing the accumulation and release of soil organic matter. However, poor understanding of the mechanisms responsible hinders the development of effective land management strategies to enhance soil carbon storage. Here we empirically test the link between microbial ecophysiological traits and topsoil carbon content across geographically distributed soils and land use contrasts. We discovered distinct pH controls on microbial mechanisms of carbon accumulation. Land use intensification in low-pH soils that increased the pH above a threshold (~6.2) leads to carbon loss through increased decomposition, following alleviation of acid retardation of microbial growth. However, loss of carbon with intensification in near-neutral pH soils was linked to decreased microbial biomass and reduced growth efficiency that was, in turn, related to trade-offs with stress alleviation and resource acquisition. Thus, less-intensive management practices in near-neutral pH soils have more potential for carbon storage through increased microbial growth efficiency, whereas in acidic soils, microbial growth is a bigger constraint on decomposition rates.White lupin (Lupinus albus L.) is an annual crop cultivated for its protein-rich seeds. It is adapted to poor soils due to the production of cluster roots, which are made of dozens of determinate lateral roots that drastically improve soil exploration and nutrient acquisition (mostly phosphate). Using long-read sequencing technologies, we provide a high-quality genome sequence of a cultivated accession of white lupin (2n\uffe2\uff80\uff89=\uffe2\uff80\uff8950, 451\uffe2\uff80\uff89Mb), as well as de novo assemblies of a landrace and a wild relative. We describe a modern accession displaying increased soil exploration capacity through early establishment of lateral and cluster roots. We also show how seed quality may have been impacted by domestication in term of protein profiles and alkaloid content. The availability of a high-quality genome assembly together with companion genomic and transcriptomic resources will enable the development of modern breeding strategies to increase and stabilize white lupin yield.

", "keywords": ["Repetitive Sequences", " Nucleic Acid/genetics", "0301 basic medicine", "[SDV]Life Sciences [q-bio]", "Plant Roots/genetics", "Gene Dosage", "Plant Science", "Crop", "Alkaloids/chemistry", "Plant Roots", "Gene", "Repetitive Sequences", "630", "Agricultural and Biological Sciences", "Domestication", "Soil", "Models", "Symbiotic Nitrogen Fixation in Legumes", "Gene Duplication", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "http://aims.fao.org/aos/agrovoc/c_3224", "Plant Proteins/metabolism", "Plant Proteins", "2. Zero hunger", "0303 health sciences", "Genome", "Q", "http://aims.fao.org/aos/agrovoc/c_27583", "Life Sciences", "Transcriptome/genetics", "http://aims.fao.org/aos/agrovoc/c_92382", "Polymorphism", " Single Nucleotide/genetics", "Lupinus", "[SDV] Life Sciences [q-bio]", "Protein Crop", "Seeds", "http://aims.fao.org/aos/agrovoc/c_5956", "White (mutation)", "Single Nucleotide/genetics", "Sequence Analysis", "Genome", " Plant", "expression des g\u00e8nes", "http://aims.fao.org/aos/agrovoc/c_4464", "Synteny/genetics", "Evolution", "Lupin Seeds", "Science", "Centromere", "Lupinus/genetics", "Polymorphism", " Single Nucleotide", "Article", "g\u00e9nomique", "Evolution", " Molecular", "Evolution and Nutritional Properties of Lupin Seeds", "physiologie v\u00e9g\u00e9tale", "03 medical and health sciences", "Alkaloids", "Genetic", "Nucleic Acid/genetics", "Seeds/physiology", "Centromere/genetics", "Genetics", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Polymorphism", "Biology", "Ecology", " Evolution", " Behavior and Systematics", "Repetitive Sequences", " Nucleic Acid", "Sequence assembly", "http://aims.fao.org/aos/agrovoc/c_25189", "Ecotype", "Models", " Genetic", "g\u00e9nome", "Botany", "Molecular", "Genetic Variation", "Molecular Sequence Annotation", "Plant", "DNA", "Sequence Analysis", " DNA", "s\u00e9quence nucl\u00e9otidique", "15. Life on land", "http://aims.fao.org/aos/agrovoc/c_27527", "Agronomy", "Plant Leaves", "Evolution and Ecology of Endophyte-Grass Symbiosis", "Lupinus albus", "FOS: Biological sciences", "Genomic Structural Variation", "Plant Leaves/metabolism", "Gene expression", "Transcriptome", "am\u00e9lioration des plantes"]}, "links": [{"href": "https://www.nature.com/articles/s41467-019-14197-9.pdf"}, {"href": "https://doi.org/10.1038/s41467-019-14197-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-019-14197-9", "name": "item", "description": "10.1038/s41467-019-14197-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-019-14197-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-24T00:00:00Z"}}, {"id": "10.1038/s41586-023-05791-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:46Z", "type": "Journal Article", "created": "2023-03-08", "title": "The giant diploid faba genome unlocks variation in a global protein crop", "description": "Abstract

Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity1. However, plant protein production is hampered by the lack of a cool-season legume equivalent to soybean in agronomic value2. Faba bean (Vicia fabaL.) has a high yield potential and is well suited for cultivation in temperate regions, but genomic resources are scarce. Here, we report a high-quality chromosome-scale assembly of the faba bean genome and show that it has expanded to a massive 13\uffe2\uff80\uff89Gb in size through an imbalance between the rates of amplification and elimination of retrotransposons and satellite repeats. Genes and recombination events are evenly dispersed across chromosomes and the gene space is remarkably compact considering the genome size, although with substantial copy number variation driven by tandem duplication. Demonstrating practical application of the genome sequence, we develop a targeted genotyping assay and use high-resolution genome-wide association analysis to dissect the genetic basis of seed size and hilum colour. The resources presented constitute a genomics-based breeding platform for faba bean, enabling breeders and geneticists to accelerate the\uffc2\uffa0improvement of sustainable protein production across the\uffc2\uffa0Mediterranean, subtropical and northern temperate agroecological zones.The present work aims to quantify the impact of climate change (CC) on the grain yields of irrigated cereals and their water requirements in the Tensift region of Morocco. The Med-CORDEX (MEDiterranean COordinated Regional Climate Downscaling EXperiment) ensemble runs under scenarios RCP4.5 (Representative Concentration Pathway) and RCP8.5 are first evaluated and disaggregated using the quantile-quantile approach. The impact of CC on the duration of the main wheat phenological stages based on the degree-day approach is then analyzed. The results show that the rise in air temperature causes a shortening of the development cycle of up to 50 days. The impacts of rising temperature and changes in precipitation on wheat yields are next evaluated, based on the AquaCrop model, both with and without taking into account the fertilizing effect of CO2. As expected, optimal wheat yields will decrease on the order of 7 to 30% if CO2 concentration rise is not considered. The fertilizing effect of CO2 can counterbalance yield losses, since optimal yields could increase by 7% and 13% respectively at mid-century for the RCP4.5 and RCP8.5 scenarios. Finally, water requirements are expected to decrease by 13 to 42%, mainly in response to the shortening of the cycle. This decrease is associated with a change in temporal patterns, with the requirement peak coming two months earlier than under current conditions.

", "keywords": ["Water resources", "Atmospheric sciences", "Agricultural Irrigation", "environment/Bioclimatology", "550", "Representative Concentration Pathways", "Adaptation to Climate Change in Agriculture", "Arid", "Rain", "[SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy", "Climate Change and Variability Research", "Plant Science", "Precipitation", "02 engineering and technology", "01 natural sciences", "Agricultural and Biological Sciences", "Downscaling", "Climate change", "Quantile", "Triticum", "Climatology", "2. Zero hunger", "Global and Planetary Change", "Ecology", "Geography", "Temperature", "Life Sciences", "Geology", "Morocco", "Phenology", "[SDV.EE.BIO]Life Sciences [q-bio]/Ecology", "Seeds", "Physical Sciences", "Metallurgy", "Desert Climate", "Impacts of Elevated CO2 and Ozone on Plant Physiology", "Climate Change", "0207 environmental engineering", "Yield (engineering)", "Climate model", "Article", "Environmental science", "FOS: Economics and business", "Meteorology", "FOS: Mathematics", "Econometrics", "[SDU.STU.HY]Sciences of the Universe [physics]/Earth Sciences/Hydrology", "Biology", "Ecology", " Evolution", " Behavior and Systematics", "0105 earth and related environmental sciences", "[SDV.SA.AGRO] Life Sciences [q-bio]/Agricultural sciences/Agronomy", "Water", "FOS: Earth and related environmental sciences", "Carbon Dioxide", "15. Life on land", "Agronomy", "Materials science", "[SDV.EE.BIO] Life Sciences [q-bio]/Ecology", " environment/Bioclimatology", "13. Climate action", "FOS: Biological sciences", "Environmental Science", "[SDU.STU.HY] Sciences of the Universe [physics]/Earth Sciences/Hydrology", "Crop Yield", "Mediterranean climate", "Mathematics", "Climate Modeling"]}, "links": [{"href": "https://www.nature.com/articles/s41598-019-55251-2.pdf"}, {"href": "https://doi.org/10.1038/s41598-019-55251-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-019-55251-2", "name": "item", "description": "10.1038/s41598-019-55251-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-019-55251-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-16T00:00:00Z"}}, {"id": "10.1051/bioconf/202516703010", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:17:56Z", "type": "Journal Article", "created": "2025-03-19", "title": "Simulation of Land Cover Change Impacts on Discharge and Sedimentation in Merawu Catchment, Indonesia", "description": "

Merawu Watershed is the upstream part of Serayu Watershed whose outlet is in Mrica Reservoir, Banjarnegara Regency. The capacity of Mrica reservoir continues to experience an increase in sedimentation from year to year, one of the main causes is the high erosion in Merawu Watershed. This study aims to determine the effect of changes in land cover on discharge and sedimentation in Merawu Watershed. Land cover changes were analyzed historically from maps from 2006 and 2022. The Merawu watershed hydrological model was built using the SWAT model and calibrated using the SUFI-2 algorithm. The effect of land cover changes was calculated by comparing the simulation results with land cover data from 2006 and 2022. The analysis of land cover changes shows that the Merawu Watershed experienced a decrease in secondary forest and cultivated land. The built SWAT model produced an R2 value of 0.6 and an NSE of 0.55 for calibration and showed an R2 value of 0.71 and an NSE of 0.69 during validation. From the simulation results the changes in land cover that occurred had an impact on an increase in the average daily discharge and sedimentation of 294.82% and 662.47%. Underlying physiological mechanisms of intraspecific variation in growth response to elevated CO2 concentration [CO2] were investigated using two spring wheat (Triticum aestivum L.) cultivars: Yitpi and H45. Leaf blade elongation rate (LER), leaf carbon (C), nitrogen (N) in the expanding leaf blade (ELB, sink) and photosynthesis (A) and C and N status in the last fully expanded leaf blade (LFELB, source) were measured. Plants were grown at ambient [CO2] (~384\uffc2\uffb5molmol\uffe2\uff80\uff931) and elevated [CO2] (~550\uffc2\uffb5molmol\uffe2\uff80\uff931) in the Australian Grains Free Air CO2 Enrichment facility. Elevated [CO2] increased leaf area and total dry mass production, respectively, by 42 and 53% for Yitpi compared with 2 and 13% for H45. Elevated [CO2] also stimulated the LER by 36% for Yitpi compared with 5% for H45. Yitpi showed a 99% increase in A at elevated [CO2] but no A stimulation was found for H45. There was a strong correlation (r2=0.807) between LER of the ELB and soluble carbohydrate concentration in LFELB. In ELB, the highest spatial N concentration was observed in the cell division zone, where N concentrations were 67.3 and 60.6mg g\uffe2\uff80\uff931 for Yitpi compared with 51.1 and 39.2mg g\uffe2\uff80\uff931 for H45 at ambient and elevated [CO2]. In contrast, C concentration increased only in the cell division and cell expansion zone of the ELB of Yitpi. These findings suggest that C supply from the source (LFELB) is cultivar dependent and well correlated with LER, leaf area expansion and whole-plant growth response to elevated [CO2].

", "keywords": ["Diurnal regulation of LER", "580", "0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "070303 Crop and Pasture Biochemistry and Physiology", "leaf blade elongation rate", "diurnal regulation of LER", "gas exchange", "Growth analysis", "15. Life on land", "01 natural sciences", "Leaf blade elongation rate", "03 medical and health sciences", "Elevated (CO2)", "elevated [CO2]", "Gas exchange", "growth analysis"]}, "links": [{"href": "https://doi.org/10.1071/fp14125"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Functional%20Plant%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1071/fp14125", "name": "item", "description": "10.1071/fp14125", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1071/fp14125"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-01T00:00:00Z"}}, {"id": "10.1093/jxb/erac437", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:18:21Z", "type": "Journal Article", "created": "2022-11-02", "title": "In situcontrol of root\u2013bacteria interactions using optical trapping in transparent soil", "description": "Abstract

Bacterial attachment on root surfaces is an important step preceding the colonization or internalization and subsequent infection of plants by pathogens. Unfortunately, bacterial attachment is not well understood because the phenomenon is difficult to observe. Here we assessed whether this limitation could be overcome using optical trapping approaches. We have developed a system based on counter-propagating beams and studied its ability to guide Pectobacterium atrosepticum (Pba) cells to different root cell types within the interstices of transparent soils. Bacterial cells were successfully trapped and guided to root hair cells, epidermal cells, border cells, and tissues damaged by laser ablation. Finally, we used the system to quantify the bacterial cell detachment rate of Pba cells on root surfaces following reversible attachment. Optical trapping techniques could greatly enhance our ability to deterministically characterize mechanisms linked to attachment and formation of biofilms in the rhizosphere.Rhizobial infection of legume roots during development of nitrogen fixing root nodules occurs either intracellularly though plant derived infection threads traversing the epidermal and cortical cell layers to deliver the bacteria or intercellularly via bacterial entry between epidermal plant cells. Although, around 25% of all legume genera are postulated to be intercellularly infected, the pathways and mechanisms supporting this process has remained virtually unexplored due to lack of genetically amenable legumes that have this infection mode. In this study, we report that the model legume Lotus japonicus is infected intercellularly by Rhizobium sp. IRBG74 and demonstrate that the resources available in Lotus enable insight into the genetic requirements and the fine-tuning of the pathway governing intercellular infection. Inoculation of Lotus mutants shows that Ern1 and RinRK1 are dispensable for intercellular infection in contrast to intracellular infection. Other symbiotic genes, including Nfr5, SymRK, CCaMK, Epr3, Cyclops, Nin, Nsp1, Nsp2, Cbs and Vpy1 are equally important for both entry modes. Comparative RNAseq analysis of roots inoculated with IRBG74 revealed a distinctive transcriptome response compared to intracellular colonization. In particular, a number of cytokinin-related genes were differentially regulated. Corroborating this observation cyp735A and ipt4 cytokinin biosynthesis mutants were significantly affected in their nodulation with IRBG74 while lhk1 cytokinin receptor mutants did not form any nodules. These results indicate that a differential requirement for cytokinin signalling conditions intercellular rhizobial entry and highlight the distinct modalities of the inter- and intra-cellular infection mechanisms.

", "keywords": ["name=Physiology", "580", "0301 basic medicine", "/dk/atira/pure/subjectarea/asjc/1300/1314", "0303 health sciences", "/dk/atira/pure/subjectarea/asjc/1300/1311", "571", "Regular Issue", "name=Genetics", "/dk/atira/pure/subjectarea/asjc/1100/1110", "Plant Roots", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Calcium-Calmodulin-Dependent Protein Kinases", "Lotus", "name=Plant Science", "Root Nodules", " Plant", "Plant Proteins", "Rhizobium", "Signal Transduction"]}, "links": [{"href": "http://academic.oup.com/plphys/article-pdf/185/3/1131/37166752/kiaa049.pdf"}, {"href": "https://doi.org/10.1093/plphys/kiaa049"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/plphys/kiaa049", "name": "item", "description": "10.1093/plphys/kiaa049", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/plphys/kiaa049"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-31T00:00:00Z"}}, {"id": "10.1093/treephys/22.7.435", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:18:23Z", "type": "Journal Article", "created": "2012-01-20", "title": "Responses Of Deciduous Broadleaf Trees To Defoliation In A Co2 Enriched Atmosphere", "description": "Relatively little is known about the implications of atmospheric CO2 enrichment for tree responses to biotic disturbances such as folivory. We examined the combined effects of elevated CO2 concentration ([CO2]) and defoliation on growth and physiology of sugar maple (Acer saccharum Marsh.) and trembling aspen (Populus tremuloides Michx.). Seedlings were planted in the ground in eight open-top chambers. Four chambers were ventilated with CO2-enriched air (ambient + 283 micromol mol-1) and four chambers were supplied with ambient air. After 6 weeks of growth, half of the leaf area was removed on a subset of seedlings of each species in each CO2 treatment. We monitored subsequent biomass gain and allocation, along with leaf gas exchange and chemistry. Defoliation did not significantly affect final seedling biomass in either species or CO2 treatment. Growth recovery following defoliation was associated with increased allocation to leaf mass in maple and a slight enhancement of mean photosynthesis in aspen. Elevated [CO2] did not significantly affect aspen growth, and the observed stimulation of maple growth was significant only in mid-season. Correspondingly, simulated responses of whole-tree photosynthesis to elevated [CO2] were constrained by a decrease in photosynthetic capacity in maple, and were partially offset by reductions in specific leaf area and biomass allocation to foliage in aspen. There was a significant interaction between [CO2] and defoliation on only a few of the measured traits. Thus, the data do not support the hypothesis that atmospheric CO2 enrichment will substantially alter tree responses to folivory. However, our findings do provide further indication that regeneration-stage growth rates of certain temperate tree species may respond only moderately to a near doubling of atmospheric [CO2].", "keywords": ["defoliation-", "0106 biological sciences", "Ecophysiology", "Quaking aspen", "biomass-allocation", "growth-response", "Growth", "Environmental-Sciences)", "01 natural sciences", "plant-composition", "Trees", "biomass-", "Spermatophyta-", "Biomass", "Photosynthesis", "plant-physiology", "defoliation", "Angiospermae-", "leaf-area", "GLOBAL-ECOLOGY", "seedling-growth", "source-sink-relations", "Populus-tremuloides", "gas-exchange", "Populus", "broadleaves-", "deciduous-tree", "forest-trees", "atmosphere-", "trees-", "biomass-production", "Acer saccharum", "Nitrogen", "Carbohydrates", "Acer", "carbon-dioxide-enrichment", "photosynthesis-", "growth-", "species-differences", "seedlings-", "wisconsin-", "Populus tremuloides", "photosynthesis", "Climatic changes", "Carbon Dioxide", "15. Life on land", "Plant Leaves", "leaves-", "Aceraceae-: Dicotyledones-", "Carbon dioxide", "Sugar maple", "Seedlings", "Terrestrial-Ecology (Ecology-", "Acer-saccharum"], "contacts": [{"organization": "Volin, John C., Kruger, Eric L., Lindroth, Richard L.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1093/treephys/22.7.435"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Tree%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/treephys/22.7.435", "name": "item", "description": "10.1093/treephys/22.7.435", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/treephys/22.7.435"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2002-05-01T00:00:00Z"}}, {"id": "10.1101/117887", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:18:26Z", "type": "Journal Article", "created": "2017-03-19", "title": "Bacterial physiological adaptations to contrasting edaphic conditions identified using landscape scale metagenomics", "description": "Abstract

Environmental factors relating to soil pH are widely known to be important in structuring soil bacterial communities, yet the relationship between taxonomic community composition and functional diversity remains to be determined. Here, we analyze geographically distributed soils spanning a wide pH gradient and assess the functional gene capacity within those communities using whole genome metagenomics. Low pH soils consistently had fewer taxa (lower alpha and gamma diversity), but only marginal reductions in functional alpha diversity and equivalent functional gamma diversity. However, coherent changes in the relative abundances of annotated genes between pH classes were identified; with functional profiles clustering according to pH independent of geography. Differences in gene abundances were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-mediated mechanisms for organic C and N substrate acquisition. These findings show that bacterial functional versatility may not be constrained by taxonomy, and we further identify the range of physiological adaptations required to exist in soils of varying nutrient availability and edaphic conditions.Rhizobial infection of legume roots during development of nitrogen fixing root nodules occurs either intracellularly though plant derived infection threads traversing the epidermal and cortical cell layers to deliver the bacteria or intercellularly via bacterial entry between epidermal plant cells. Although, around 25% of all legume genera are postulated to be intercellularly infected, the pathways and mechanisms supporting this process has remained virtually unexplored due to lack of genetically amenable legumes that have this infection mode. In this study, we report that the model legume Lotus japonicus is infected intercellularly by Rhizobium sp. IRBG74 and demonstrate that the resources available in Lotus enable insight into the genetic requirements and the fine-tuning of the pathway governing intercellular infection. Inoculation of Lotus mutants shows that Ern1 and RinRK1 are dispensable for intercellular infection in contrast to intracellular infection. Other symbiotic genes, including Nfr5, SymRK, CCaMK, Epr3, Cyclops, Nin, Nsp1, Nsp2, Cbs and Vpy1 are equally important for both entry modes. Comparative RNAseq analysis of roots inoculated with IRBG74 revealed a distinctive transcriptome response compared to intracellular colonization. In particular, a number of cytokinin-related genes were differentially regulated. Corroborating this observation cyp735A and ipt4 cytokinin biosynthesis mutants were significantly affected in their nodulation with IRBG74 while lhk1 cytokinin receptor mutants did not form any nodules. These results indicate that a differential requirement for cytokinin signalling conditions intercellular rhizobial entry and highlight the distinct modalities of the inter- and intra-cellular infection mechanisms.The rate of change (RoC) of environmental drivers matters: biotic and abiotic components respond differently when faced with a fast or slow change in their environment. This phenomenon occurs across spatial scales and thus levels of ecological organization. We investigated the RoC of environmental drivers in the ecological literature and examined publication trends across ecological levels, including prevalent types of evidence and drivers. Research interest in environmental driver RoC has increased over time (particularly in the last decade), however, the amount of research and type of studies were not equally distributed across levels of organization and different subfields of ecology use temporal terminology (e.g. \uffe2\uff80\uff98abrupt\uffe2\uff80\uff99 and \uffe2\uff80\uff98gradual\uffe2\uff80\uff99) differently, making it difficult to compare studies. At the level of individual organisms, evidence indicates that responses and underlying mechanisms are different when environmental driver treatments are applied at different rates, thus we propose including a time dimension into reaction norms. There is much less experimental evidence at higher levels of ecological organization (i.e. population, community, ecosystem), although theoretical work at the population level indicates the importance of RoC for evolutionary responses. We identified very few studies at the community and ecosystem levels, although existing evidence indicates that driver RoC is important at these scales and potentially could be particularly important for some processes, such as community stability and cascade effects. We recommend shifting from a categorical (e.g. abrupt versus gradual) to a quantitative and continuous (e.g. \uffc2\uffb0C/h) RoC framework and explicit reporting of RoC parameters, including magnitude, duration and start and end points to ease cross\uffe2\uff80\uff90scale synthesis and alleviate ambiguity. Understanding how driver RoC affects individuals, populations, communities and ecosystems, and furthermore how these effects can feed back between levels is critical to making improved predictions about ecological responses to global change drivers. The application of a unified quantitative RoC framework for ecological studies investigating environmental driver RoC will both allow cross\uffe2\uff80\uff90scale synthesis to be accomplished more easily and has the potential for the generation of novel hypotheses.

", "keywords": ["0301 basic medicine", "570", "0303 health sciences", "Ecology", "abrupt", "rate of change", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie", "gradual", "15. Life on land", "03 medical and health sciences", "13. Climate action", "physiology", "Humans", "ecology", "Ecosystem", "global change", "time"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/brv.12639"}, {"href": "https://doi.org/10.1111/brv.12639"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biological%20Reviews", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/brv.12639", "name": "item", "description": "10.1111/brv.12639", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/brv.12639"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-05T00:00:00Z"}}, {"id": "10.1111/gcb.13893", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:18:39Z", "type": "Journal Article", "created": "2017-09-06", "title": "Towards physiologically meaningful water-use efficiency estimates from eddy covariance data", "description": "Abstract

Intrinsic water\uffe2\uff80\uff90use efficiency (iWUE) characterizes the physiological control on the simultaneous exchange of water and carbon dioxide in terrestrial ecosystems. Knowledge of iWUE is commonly gained from leaf\uffe2\uff80\uff90level gas exchange measurements, which are inevitably restricted in their spatial and temporal coverage. Flux measurements based on the eddy covariance (EC) technique can overcome these limitations, as they provide continuous and long\uffe2\uff80\uff90term records of carbon and water fluxes at the ecosystem scale. However, vegetation gas exchange parameters derived from EC data are subject to scale\uffe2\uff80\uff90dependent and method\uffe2\uff80\uff90specific uncertainties that compromise their ecophysiological interpretation as well as their comparability among ecosystems and across spatial scales. Here, we use estimates of canopy conductance and gross primary productivity (GPP) derived from EC data to calculate a measure of iWUE (G1, \uffe2\uff80\uff9cstomatal slope\uffe2\uff80\uff9d) at the ecosystem level at six sites comprising tropical, Mediterranean, temperate, and boreal forests. We assess the following six mechanisms potentially causing discrepancies between leaf and ecosystem\uffe2\uff80\uff90level estimates of G1: (i) non\uffe2\uff80\uff90transpirational water fluxes; (ii) aerodynamic conductance; (iii) meteorological deviations between measurement height and canopy surface; (iv) energy balance non\uffe2\uff80\uff90closure; (v) uncertainties in net ecosystem exchange partitioning; and (vi) physiological within\uffe2\uff80\uff90canopy gradients. Our results demonstrate that an unclosed energy balance caused the largest uncertainties, in particular if it was associated with erroneous latent heat flux estimates. The effect of aerodynamic conductance on G1 was sufficiently captured with a simple representation. G1 was found to be less sensitive to meteorological deviations between canopy surface and measurement height and, given that data are appropriately filtered, to non\uffe2\uff80\uff90transpirational water fluxes. Uncertainties in the derived GPP and physiological within\uffe2\uff80\uff90canopy gradients and their implications for parameter estimates at leaf and ecosystem level are discussed. Our results highlight the importance of adequately considering the sources of uncertainty outlined here when EC\uffe2\uff80\uff90derived water\uffe2\uff80\uff90use efficiency is interpreted in an ecophysiological context.

", "keywords": ["550", "ecophysiology", "Penman\u2013Monteith equation", "0207 environmental engineering", "577", "slope parameter", "02 engineering and technology", "Forests", "Models", " Biological", "01 natural sciences", "Trees", "Water Cycle", "XXXXXX - Unknown", "eddy covariance", "energy imbalance", "analysis of covariance", "0105 earth and related environmental sciences", "intrinsic water-use efficiency", "Water", "eddy flux", "Plant Transpiration", "Carbon Dioxide", "15. Life on land", "aerodynamic conductance", "water efficiency", "Carbon", "6. Clean water", "canopy gradients", "surface conductance", "Plant Leaves", "13. Climate action", "ecosystems"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.13893"}, {"href": "https://doi.org/10.1111/gcb.13893"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.13893", "name": "item", "description": "10.1111/gcb.13893", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.13893"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-10-11T00:00:00Z"}}, {"id": "10.1111/j.1365-3040.2010.02201.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:18:51Z", "type": "Journal Article", "created": "2010-06-21", "title": "Soil [N] modulates soil C cycling in CO2-fumigated tree stands: a meta-analysis", "description": "ABSTRACT

Under elevated atmospheric CO2 concentrations, soil carbon (C) inputs are typically enhanced, suggesting larger soil C sequestration potential. However, soil C losses also increase and progressive nitrogen (N) limitation to plant growth may reduce the CO2 effect on soil C inputs with time. We compiled a data set from 131 manipulation experiments, and used meta\uffe2\uff80\uff90analysis to test the hypotheses that: (1) elevated atmospheric CO2 stimulates soil C inputs more than C losses, resulting in increasing soil C stocks; and (2) that these responses are modulated by N. Our results confirm that elevated CO2 induces a C allocation shift towards below\uffe2\uff80\uff90ground biomass compartments. However, the increased soil C inputs were offset by increased heterotrophic respiration (Rh), such that soil C content was not affected by elevated CO2. Soil N concentration strongly interacted with CO2 fumigation: the effect of elevated CO2 on fine root biomass and \uffe2\uff80\uff93production and on microbial activity increased with increasing soil N concentration, while the effect on soil C content decreased with increasing soil N concentration. These results suggest that both plant growth and microbial activity responses to elevated CO2 are modulated by N availability, and that it is essential to account for soil N concentration in C cycling analyses.

", "keywords": ["0301 basic medicine", "Physiology", "Plant Science", "Fine root production", "Carbon Cycle", "Trees", "Soil", "03 medical and health sciences", "Microbial respiration", "microbial respiration", "XXXXXX - Unknown", "C sequestration", "SDG 13 - Climate Action", "Biomass", "Fertilizers", "Biology", "[CO] enrichment", "2. Zero hunger", "[SDU.OCEAN]Sciences of the Universe [physics]/Ocean", "0303 health sciences", "biomass", "[SDU.OCEAN] Sciences of the Universe [physics]/Ocean", " Atmosphere", "Atmosphere", "Root biomass", "Carbon Dioxide", "Nitrogen Cycle", "15. Life on land", "carbon sequestration", "N fertilization", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "nitrogen fertilizers", "roots (botany)", "13. Climate action", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "environment"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-3040.2010.02201.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%2C%20Cell%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-3040.2010.02201.x", "name": "item", "description": "10.1111/j.1365-3040.2010.02201.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-3040.2010.02201.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-11-12T00:00:00Z"}}, {"id": "10.1111/nph.14288", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:18:59Z", "type": "Journal Article", "created": "2016-11-07", "title": "The response of ecosystem water-use efficiency to rising atmospheric CO 2 concentrations: sensitivity and large-scale biogeochemical implications", "description": "Summary

Ecosystem water\uffe2\uff80\uff90use efficiency (WUE) is an important metric linking the global land carbon and water cycles. Eddy covariance\uffe2\uff80\uff90based estimates of WUE in temperate/boreal forests have recently been found to show a strong and unexpected increase over the 1992\uffe2\uff80\uff932010 period, which has been attributed to the effects of rising atmospheric CO2 concentrations on plant physiology.

To test this hypothesis, we forced the observed trend in the process\uffe2\uff80\uff90based land surface model JSBACH by increasing the sensitivity of stomatal conductance (gs) to atmospheric CO2 concentration. We compared the simulated continental discharge, evapotranspiration (ET), and the seasonal CO2 exchange with observations across the extratropical northern hemisphere.

The increased simulated WUE led to substantial changes in surface hydrology at the continental scale, including a significant decrease in ET and a significant increase in continental runoff, both of which are inconsistent with large\uffe2\uff80\uff90scale observations. The simulated seasonal amplitude of atmospheric CO2 decreased over time, in contrast to the observed upward trend across ground\uffe2\uff80\uff90based measurement sites.

Our results provide strong indications that the recent, large\uffe2\uff80\uff90scale WUE trend is considerably smaller than that estimated for these forest ecosystems. They emphasize the decreasing CO2 sensitivity of WUE with increasing scale, which affects the physiological interpretation of changes in ecosystem WUE.

", "keywords": ["580", "0106 biological sciences", "Time Factors", "plant physiology", "Vapor Pressure", "evapotranspiration", "577", "Water", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "01 natural sciences", "water efficiency", "3. Good health", "Plant Leaves", "13. Climate action", "atmospheric carbon dioxide", "XXXXXX - Unknown", "0401 agriculture", " forestry", " and fisheries", "Seasons", "ecosystems", "Ecosystem", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14288"}, {"href": "https://doi.org/10.1111/nph.14288"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.14288", "name": "item", "description": "10.1111/nph.14288", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.14288"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-11-07T00:00:00Z"}}, {"id": "10.1111/nph.14634", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:18:59Z", "type": "Journal Article", "created": "2017-06-13", "title": "Circular linkages between soil biodiversity, fertility and plant productivity are limited to topsoil at the continental scale", "description": "Summary

The current theoretical framework suggests that tripartite positive feedback relationships between soil biodiversity, fertility and plant productivity are universal. However, empirical evidence for these relationships at the continental scale and across different soil depths is lacking.

We investigate the continental\uffe2\uff80\uff90scale relationships between the diversity of microbial and invertebrate\uffe2\uff80\uff90based soil food webs, fertility and above\uffe2\uff80\uff90ground plant productivity at 289 sites and two soil depths, that is 0\uffe2\uff80\uff9310 and 20\uffe2\uff80\uff9330\uffc2\uffa0cm, across Australia.

Soil biodiversity, fertility and plant productivity are strongly positively related in surface soils. Conversely, in the deeper soil layer, the relationships between soil biodiversity, fertility and plant productivity weaken considerably, probably as a result of a reduction in biodiversity and fertility with depth. Further modeling suggested that strong positive associations among soil biodiversity\uffe2\uff80\uff93fertility and fertility\uffe2\uff80\uff93plant productivity are limited to the upper soil layer (0\uffe2\uff80\uff9310\uffc2\uffa0cm), after accounting for key factors, such as distance from the equator, altitude, climate and physicochemical soil properties.

These findings highlight the importance of surface soil biodiversity for soil fertility, and suggest that any loss of surface soil could potentially break the links between soil biodiversity\uffe2\uff80\uff93fertility and/or fertility\uffe2\uff80\uff93plant productivity, which can negatively impact nutrient cycling and food production, upon which future generations depend.

Soil adjacent to roots has distinct structural and physical properties from bulk soil, affecting water and solute acquisition by plants. Detailed knowledge on how root activity and traits such as root hairs affect the three\uffe2\uff80\uff90dimensional pore structure at a fine scale is scarce and often contradictory.

Roots of hairless barley (Hordeum vulgare L. cv Optic) mutant (NRH) and its wildtype (WT) parent were grown in tubes of sieved (<250\uffc2\uffa0\uffce\uffbcm) sandy loam soil under two different water regimes. The tubes were scanned by synchrotron\uffe2\uff80\uff90based X\uffe2\uff80\uff90ray computed tomography to visualise pore structure at the soil\uffe2\uff80\uff93root interface. Pore volume fraction and pore size distribution were analysed vs distance within 1\uffc2\uffa0mm of the root surface.

Less dense packing of particles at the root surface was hypothesised to cause the observed increased pore volume fraction immediately next to the epidermis. The pore size distribution was narrower due to a decreased fraction of larger pores. There were no statistically significant differences in pore structure between genotypes or moisture conditions.

A model is proposed that describes the variation in porosity near roots taking into account soil compaction and the surface effect at the root surface.

", "keywords": ["name=Physiology", "STABILIZATION", "Physiology", "EP/M020355/1", "Supplementary Data", "QH301 Biology", "Plant Science", "Supplementary data available", "Plant Roots", "630", "noninvasive imaging", "Soil", "646809DIMR", "STRENGTH", "BB/J00868/1", "Hordeum vulgare", "2. Zero hunger", "04 agricultural and veterinary sciences", "Rhizosphere", "COMPRESSION", "soil structure", "Porosity", "European Research Council", "/dk/atira/pure/subjectarea/asjc/1300/1314", "/dk/atira/pure/subjectarea/asjc/1100/1110", "root hairs", "COMPACTION", "QH301", "Imaging", " Three-Dimensional", "synchrotron", "particle packing", "SOIL-STRUCTURE", "BB/L025620/1", "WATER-STRESS", "NE/L00237/1", "580", "ELONGATION", "Civil_env_eng", "Natural Environment Research Council (NERC)", "POROSITY", "Water", "Hordeum", "15. Life on land", "Engineering and Physical Sciences Research Council (EPSRC)", "Mutation", "Biotechnology and Biological Sciences Research Council (BBSRC)", "0401 agriculture", " forestry", " and fisheries", "PENETRATION", "name=Plant Science", "rhizosphere", "Tomography", " X-Ray Computed", "MAIZE", "BB/P004180/1", "Synchrotrons", "BB/L025825/1"]}, "links": [{"href": "https://repository.uwl.ac.uk/id/eprint/5489/1/AS6808504337817661539338801587_content_1.pdf"}, {"href": "https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.15516"}, {"href": "https://doi.org/10.1111/nph.15516"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.15516", "name": "item", "description": "10.1111/nph.15516", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.15516"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-11-20T00:00:00Z"}}, {"id": "10.1111/nph.16312", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:19:00Z", "type": "Journal Article", "created": "2019-11-06", "title": "The helical motions of roots are linked to avoidance of particle forces in soil", "description": "Summary

Limitation to root growth results from forces required to overcome soil resistance to deformation. The variations in individual particle forces affects root development and often deflects the growth trajectory.

We have developed transparent soil and optical projection tomography microscopy systems where measurements of growth trajectory and particle forces can be acquired in a granular medium at a range of confining pressures. We developed image\uffe2\uff80\uff90processing pipelines to analyse patterns in root trajectories and a stochastic\uffe2\uff80\uff90mechanical theory to establish how root deflections relate to particle forces and thickening of the root.

Root thickening compensates for the increase in mean particle forces but does not prevent deflections from 5% of most extreme individual particle forces causing root deflection. The magnitude of deflections increases with pressure but they assemble into helices of conserved wavelength in response linked to gravitropism.

The study reveals mechanisms for the understanding of root growth in mechanically impeding soil conditions and provides insights relevant to breeding of drought\uffe2\uff80\uff90resistant crops.

Feedback between plants and soil microbial communities can be a powerful driver of vegetation dynamics. Plants elicit changes in the soil microbiome that either promote or suppress conspecifics at the same location, thereby regulating population density\uffe2\uff80\uff90dependence and species co\uffe2\uff80\uff90existence. Such effects are often attributed to the accumulation of host\uffe2\uff80\uff90specific antagonistic or beneficial microbiota in the rhizosphere. However, the identity and host\uffe2\uff80\uff90specificity of the microbial taxa involved are rarely empirically assessed. Here we review the evidence for host\uffe2\uff80\uff90specificity in plant\uffe2\uff80\uff90associated microbes and propose that specific plant\uffe2\uff80\uff93soil feedbacks can also be driven by generalists. We outline the potential mechanisms by which generalist microbial pathogens, mutualists and decomposers can generate differential effects on plant hosts and synthesize existing evidence to predict these effects as a function of plant investments into defence, microbial mutualists and dispersal. Importantly, the capacity of generalist microbiota to drive plant\uffe2\uff80\uff93soil feedbacks depends not only on the traits of individual plants but also on the phylogenetic and functional diversity of plant communities. Identifying factors that promote specialization or generalism in plant\uffe2\uff80\uff93microbial interactions and thereby modulate the impact of microbiota on plant performance will advance our understanding of the mechanisms underlying plant\uffe2\uff80\uff93soil feedback and the ways it contributes to plant co\uffe2\uff80\uff90existence.p1Plants transitioned from an aquatic to a terrestrial lifestyle during their evolution. On land, fluctuations on water availability in the environment became one of the major problems they encountered. The appearance of morpho-physiological adaptations to cope with and tolerate water loss from the cells was undeniably useful to survive on dry land. Some of these adaptations, such as carbon concentrating mechanisms (CCMs), desiccation tolerance (DT) and root impermeabilization, appeared in multiple plant lineages. Despite being crucial for evolution on land, it has been unclear how these adaptations convergently evolved in the various plant lineages. Recent advances on whole genome and transcriptome sequencing are revealing that co-option of genes and gene regulatory networks (GRNs) is a common feature underlying the convergent evolution of these adaptations. In this review we address how the study of CCMs and DT have provided insight into convergent evolution of GRNs underlying plant adaptation to dry environments, and how these insights could be applied to currently emerging understanding of evolution of root impermeabilization through different barrier cell types. We discuss examples of co-option, conservation, and innovation of genes and GRNs at the cell, tissue and organ levels revealed by recent phylogenomic (comparative genomic) and comparative transcriptomic studies.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Physiology", "desiccation tolerance", "exodermis", "Adaptation", " Biological", "Reviews", "Plant Science", "comparative genomics", "Plants", "15. Life on land", "Genes", " Plant", "Biological Evolution", "03 medical and health sciences", "apoplastic barriers", "Gene Regulatory Networks", "Desert Climate"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.14143"}, {"href": "https://doi.org/10.1111/pce.14143"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%2C%20Cell%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/pce.14143", "name": "item", "description": "10.1111/pce.14143", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/pce.14143"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-17T00:00:00Z"}}, {"id": "10.1128/mBio.00799-17", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:19:06Z", "type": "Journal Article", "created": "2017-03-19", "title": "Bacterial Physiological Adaptations to Contrasting Edaphic Conditions Identified Using Landscape Scale Metagenomics", "description": "Abstract

Environmental factors relating to soil pH are widely known to be important in structuring soil bacterial communities, yet the relationship between taxonomic community composition and functional diversity remains to be determined. Here, we analyze geographically distributed soils spanning a wide pH gradient and assess the functional gene capacity within those communities using whole genome metagenomics. Low pH soils consistently had fewer taxa (lower alpha and gamma diversity), but only marginal reductions in functional alpha diversity and equivalent functional gamma diversity. However, coherent changes in the relative abundances of annotated genes between pH classes were identified; with functional profiles clustering according to pH independent of geography. Differences in gene abundances were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-mediated mechanisms for organic C and N substrate acquisition. These findings show that bacterial functional versatility may not be constrained by taxonomy, and we further identify the range of physiological adaptations required to exist in soils of varying nutrient availability and edaphic conditions.

", "keywords": ["Q Science", "0301 basic medicine", "330", "Supplementary Data", "ecophysiology", "Ecophysiology", "NE/E006353/1", "Bacterial Physiological Phenomena", "Microbiology", "Soil", "03 medical and health sciences", "Virology", "European Commission", "Ecosystem", "Phylogeny", "Soil Microbiology", "2. Zero hunger", "655240", "metagenomics", "0303 health sciences", "Bacteria", "Natural Environment Research Council (NERC)", "Q", "NE/M017125/1", "Biodiversity", "Hydrogen-Ion Concentration", "15. Life on land", "Adaptation", " Physiological", "soil microbiology", "QR1-502", "United Kingdom", "3. Good health", "Soil microbiology", "Metagenomics", "Genome", " Bacterial", "Research Article"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/117887v1.full.pdf"}, {"href": "https://journals.asm.org/doi/pdf/10.1128/mBio.00799-17"}, {"href": "https://doi.org/10.1128/mBio.00799-17"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mBio", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/mBio.00799-17", "name": "item", "description": "10.1128/mBio.00799-17", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/mBio.00799-17"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-03-18T00:00:00Z"}}, {"id": "10.1126/science.ade9204", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:19:05Z", "type": "Journal Article", "created": "2023-01-19", "title": "Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis", "description": "

Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals. Due to recent improvements, Nanopore sequencing has become a promising method for experiments relying on amplicon sequencing. We describe a flexible workflow to generate and annotate high-quality, full-length 16S rDNA amplicons. We evaluated it for two applications, namely, (i) identification of bacterial isolates and (ii) species-level profiling of microbial communities. We assessed the identification of single bacterial isolates by sequencing, using a set of barcoded full-length 16S rRNA gene primer pairs (pair A), on 47 isolates encompassing multiple genera and compared those results with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification. Species-level community profiling was tested with two sets of barcoded full-length 16S primer pairs (A and B) and compared to the results obtained with shotgun Illumina sequencing using 27 stool samples. We developed a Nextflow pipeline to retain high-quality reads and taxonomically annotate them. We found high agreement between our workflow and MALDI-TOF data for isolate identification (positive predictive value = 0.90, Cram\uffc3\uffa9r\uffe2\uff80\uff99s V = 0.857, and Theil\uffe2\uff80\uff99s U = 0.316). For species-level community profiling, we found strong correlations ( r s > 0.6) of alpha diversity indices between the two primer sets and Illumina sequencing. At the community level, we found significant but small differences when comparing sequencing techniques. Finally, we found a moderate to strong correlation when comparing the relative abundances of individual species (average r s = 0.6 and 0.533 for primers A and B). Despite identified shortcomings, the proposed workflow enabled accurate identification of single bacterial isolates and prominent features in microbial communities, making it a worthwhile alternative to MALDI-TOF MS and Illumina sequencing.

IMPORTANCE

A quick, robust, simple, and cost-effective method to identify bacterial isolates and communities in each sample is indispensable in the fields of microbiology and infection biology. Recent technological advances in Oxford Nanopore Technologies sequencing make this technique an attractive option considering the adaptability, portability, and cost-effectiveness of the platform, even with small sequencing batches. Here, we validated a flexible workflow to identify bacterial isolates and characterize bacterial communities using the Oxford Nanopore Technologies sequencing platform combined with the most recent v14 chemistry kits. For bacterial isolates, we compared our nanopore-based approach to matrix-assisted laser desorption ionization-time of flight mass spectrometry-based identification. For species-level profiling of complex bacterial communities, we compared our nanopore-based approach to Illumina shotgun sequencing. For reproducibility purposes, we wrapped the code used to process the sequencing data into a ready-to-use and self-contained Nextflow pipeline.

", "keywords": ["DNA", " Bacterial", "1303 Biochemistry", "gut microbiome", "610 Medicine & health", "Microbiology", "Workflow", "1311 Genetics", "RNA", " Ribosomal", " 16S", "1312 Molecular Biology", "1706 Computer Science Applications", "DNA Barcoding", " Taxonomic", "Humans", "DNA sequencing", "Bacteria", "10179 Institute of Medical Microbiology", "Microbiota", "2404 Microbiology", "1314 Physiology", "bioinformatics", "QR1-502", "Nanopore Sequencing", "1105 Ecology", " Evolution", " Behavior and Systematics", "Spectrometry", " Mass", " Matrix-Assisted Laser Desorption-Ionization", "570 Life sciences; biology", "2611 Modeling and Simulation", "Research Article"]}, "links": [{"href": "https://doi.org/10.1128/msystems.00859-24"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00859-24", "name": "item", "description": "10.1128/msystems.00859-24", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00859-24"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-04-11T00:00:00Z"}}, {"id": "10.1128/aem.69.3.1800-1809.2003", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:19:06Z", "type": "Journal Article", "created": "2003-03-06", "title": "Soil Type Is The Primary Determinant Of The Composition Of The Total And Active Bacterial Communities In Arable Soils", "description": "ABSTRACT

Degradation of agricultural land and the resulting loss of soil biodiversity and productivity are of great concern. Land-use management practices can be used to ameliorate such degradation. The soil bacterial communities at three separate arable farms in eastern England, with different farm management practices, were investigated by using a polyphasic approach combining traditional soil analyses, physiological analysis, and nucleic acid profiling. Organic farming did not necessarily result in elevated organic matter levels; instead, a strong association with increased nitrate availability was apparent. Ordination of the physiological (BIOLOG) data separated the soil bacterial communities into two clusters, determined by soil type. Denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism analyses of 16S ribosomal DNA identified three bacterial communities largely on the basis of soil type but with discrimination for pea cropping. Five fields from geographically distinct soils, with different cropping regimens, produced highly similar profiles. The active communities (16S rRNA) were further discriminated by farm location and, to some degree, by land-use practices. The results of this investigation indicated that soil type was the key factor determining bacterial community composition in these arable soils. Leguminous crops on particular soil types had a positive effect upon organic matter levels and resulted in small changes in the active bacterial population. The active population was therefore more indicative of short-term management changes.

", "keywords": ["Polymerase Chain Reaction", "geography", "630", "1000 Technology", "Soil", "soil type", "RNA", " Ribosomal", " 16S", "C500 - Microbiology", "genetic polymorphism", "soil analysis", "Bacteria (microorganisms)", "Soil Microbiology", "2. Zero hunger", "article", "Agriculture", "Fabaceae", "Biodiversity", "legume", "04 agricultural and veterinary sciences", "Bacterial Typing Techniques", "microbial community", "Polymorphism", " Restriction Fragment Length", "0605 Microbiology", "Electrophoresis", "16S", "570", "Conservation of Natural Resources", "productivity", "RNA 16S", "soil microorganism", "0600 Biological Sciences", "DNA", " Ribosomal", "0700 Agricultural And Veterinary Sciences", "controlled study", "community composition", "Polymorphism", "Pisum sativum", "Ecosystem", "Ribosomal", "nonhuman", "Bacteria", "bacterial flora", "land use", "DNA", "15. Life on land", "bacterial disease", "Restriction Fragment Length", "C180 - Ecology", "physiology", "RNA", "Soils", "0401 agriculture", " forestry", " and fisheries", "bioavailability"]}, "links": [{"href": "https://doi.org/10.1128/aem.69.3.1800-1809.2003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20and%20Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/aem.69.3.1800-1809.2003", "name": "item", "description": "10.1128/aem.69.3.1800-1809.2003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/aem.69.3.1800-1809.2003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-03-01T00:00:00Z"}}, {"id": "10.20944/preprints202410.1469.v1", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:19:49Z", "type": "Journal Article", "created": "2024-10-22", "title": "Impact of Legacy Perfluorooctane Sulfonate (PFOS) and Perfluorooctanoate (PFOA) on GABA Receptor-Mediated Currents in Neuron-like Neuroblastoma Cells: Insights into Neurotoxic Mechanisms and Health Implications", "description": "

Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) are persistent environmental pollutants, raising concerns due to their widespread presence and disruptive biological effects. These compounds are highly stable, allowing them to bioaccumulate in the environment and living organisms, potentially impacting critical physiological functions such as hormonal balance, immune response, and increasing cancer risk. Despite regulatory restrictions, their pervasive nature necessitates further research into their potential effects on cellular and neuronal function. This study first evaluated the cytotoxic effects of PFOS and PFOA on S1 neuroblastoma cells, revealing a dose-dependent reduction in cell viability for PFOS, while PFOA exhibited minimal toxicity until millimolar concentrations. We further investigated their potential to modulate GABAergic neurotransmission using patch-clamp electrophysiology. Both PFOS and PFOA caused a significant, but reversible, reduction in GABA receptor-mediated currents following one-minute pre-treatment. These findings suggest that PFOS and PFOA can interfere with both cellular viability and GABAergic signaling, providing critical insights into their functional impacts and highlighting the need for further investigation into the long-term consequences of PFAS exposure on nervous system health.

", "keywords": ["electrophysiology", " GABAergic signaling", " neurotoxicity", " PFAS", " persistence", " toxicology", "RA1190-1270", "neurotoxicity", "PFAS", "Toxicology. Poisons", "610", "persistence", "Therapeutics. Pharmacology", "RM1-950", "electrophysiology", "GABAergic signaling", "Article", "toxicology"], "contacts": [{"organization": "Laura Lagostena, Davide Rotondo, Davide Gualandris, Antonio Calisi, Candida Lorusso, Valeria Magnelli, Francesco Dondero,", "roles": ["creator"]}]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/511081/1/jox-14-00094.pdf"}, {"href": "https://www.mdpi.com/2039-4713/14/4/94/pdf"}, {"href": "https://doi.org/10.20944/preprints202410.1469.v1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Xenobiotics", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.20944/preprints202410.1469.v1", "name": "item", "description": "10.20944/preprints202410.1469.v1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.20944/preprints202410.1469.v1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-21T00:00:00Z"}}, {"id": "10.22541/au.162389526.68400631/v1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:20:15Z", "type": "Journal Article", "created": "2021-06-17", "title": "Convergent evolution of gene regulatory networks underlying plant adaptations to dry environments", "description": "

Plants transitioned from an aquatic to a terrestrial lifestyle during their evolution. On land, fluctuations on water availability in the environment became one of the major problems they encountered. The appearance of morpho-physiological adaptations to cope with and tolerate water loss from the cells was undeniably useful to survive on dry land. Some of these adaptations, such as carbon concentrating mechanisms (CCMs), desiccation tolerance (DT) and root impermeabilization, appeared in multiple plant lineages. Despite being crucial for evolution on land, it has been unclear how these adaptations convergently evolved in the various plant lineages. Recent advances on whole genome and transcriptome sequencing are revealing that co-option of genes and gene regulatory networks (GRNs) is a common feature underlying the convergent evolution of these adaptations. In this review we address how the study of CCMs and DT have provided insight into convergent evolution of GRNs underlying plant adaptation to dry environments, and how these insights could be applied to currently emerging understanding of evolution of root impermeabilization through different barrier cell types. We discuss examples of co-option, conservation, and innovation of genes and GRNs at the cell, tissue and organ levels revealed by recent phylogenomic (comparative genomic) and comparative transcriptomic studies.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Physiology", "desiccation tolerance", "exodermis", "Adaptation", " Biological", "Reviews", "Plant Science", "comparative genomics", "Plants", "15. Life on land", "Genes", " Plant", "Biological Evolution", "03 medical and health sciences", "apoplastic barriers", "Gene Regulatory Networks", "Desert Climate"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.14143"}, {"href": "https://doi.org/10.22541/au.162389526.68400631/v1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%2C%20Cell%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.22541/au.162389526.68400631/v1", "name": "item", "description": "10.22541/au.162389526.68400631/v1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.22541/au.162389526.68400631/v1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-17T00:00:00Z"}}, {"id": "10.3389/fmicb.2020.562238", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:20:30Z", "type": "Journal Article", "created": "2020-09-22", "title": "The Free-Living Stage Growth Conditions of the Endophytic Fungus Serendipita indica May Regulate Its Potential as Plant Growth Promoting Microbe", "description": "Serendipita indica (former Piriformospora indica) is a non-obligate endophytic fungus and generally a plant growth and defence promoter with high potential to be used in agriculture. However, S. indica may switch from biotrophy to saprotrophy losing its plant growth promoting traits. Our aim was to understand if the free-living stage growth conditions (namely C availability) regulate S. indica's phenotype, and its potential as plant-growth-promoting-microbe (PGPM). We grew S. indica in its free-living stage under increasing C availabilities (2-20 g L-1 of glucose or sucrose). We first characterised the effect of C availability during free-living stage growth on fungal phenotype: colonies growth and physiology (plasma membrane proton pumps, stable isotopic signatures, and potential extracellular decomposing enzymes). The effect of the C availability during the free-living stage of the PGPM was evaluated on wheat. We observed that C availability during the free-living stage regulated S. indica's growth, ultrastructure and physiology, resulting in two distinct colony phenotypes: compact and explorer. The compact phenotype developed at low C, used peptone as the major C and N source, and displayed higher decomposing potential for C providing substrates; while the explorer phenotype developed at high C, used glucose and sucrose as major C sources and casein and yeast extract as major N sources, and displayed higher decomposing potential for N and P providing substrates. The C availability, or the C/N ratio, during the free-living stage left a legacy to the symbiosis stage, regulating S. indica's potential to promote plant growth: wheat growth promotion by the explorer phenotype was \u00b1 40% higher than that by the compact phenotype. Our study highlights the importance of considering microbial ecology in designing PGPM/biofertilizers. Further studies are needed to test the phenotypes under more extreme conditions, and to understand if the in vitro acquired characteristics persist under field conditions.", "keywords": ["free-living stage", "symbiosis stage", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "fungal phenotype", "morphology", "physiology", "plant-growth-promoting-microbes", "15. Life on land", "Microbiology", "QR1-502"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2020.562238"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2020.562238", "name": "item", "description": "10.3389/fmicb.2020.562238", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2020.562238"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-09-22T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Physiology&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Physiology&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Physiology&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Physiology&offset=50", "hreflang": "en-US"}], "numberMatched": 98, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-07-28T22:59:46.463243Z"}