{"type": "FeatureCollection", "features": [{"id": "10.1016/j.jhazmat.2021.126527", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:17:16Z", "type": "Journal Article", "created": "2021-06-29", "title": "Reduction of antibiotic resistance determinants in urban wastewater by ozone: Emphasis on the impact of wastewater matrix towards the inactivation kinetics, toxicity and bacterial regrowth", "description": "This study investigated the impact of bench-scale ozonation on the inactivation of total cultivable and antibiotic-resistant bacteria (faecal coliforms, Escherichia coli, Pseudomonas aeruginosa, Enterococcus spp., and total heterotrophs), and the reduction of gene markers (16S rRNA and intl1) and antibiotic resistance genes (qacE\u03941, sul1, aadA1 and dfrA1) indigenously present in wastewater effluents treated by membrane bioreactor (MBR) or conventional activated sludge (CAS). The Chick-Watson model-predicted ozone exposure (CT) requirements, showed that higher CT values were needed for CAS- than MBR-treated effluents to achieve a 3-log reduction of each microbial group, i.e., ~30 and 10 gO3 min gDOC-1 respectively. Ozonation was efficient in inactivating the examined antibiotic-resistant bacteria, and no bacterial regrowth was observed after 72\u00a0h. The genes abundance decreased significantly by ozone, but an increase in their abundance was detected 72\u00a0h after storage of the treated samples. A very low removal of DOC was achieved and at the same time phyto- and eco-toxicity increased after the ozonation treatment in both wastewater matrices. The gene abundance, regrowth and toxicity results of this study may be of high environmental significance for comprehensive evaluation of ozone and may guide future studies in assessing these parameters for other oxidants/disinfectants.", "keywords": ["Bacteria", "0211 other engineering and technologies", "Drug Resistance", " Microbial", "02 engineering and technology", "Wastewater", "Waste Disposal", " Fluid", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Disinfection", "Kinetics", "Ozone", "Genes", "13. Climate action", "Ozonation", "Phytotoxicity", "RNA", " Ribosomal", " 16S", "11. 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However, environmental and quality assessments are needed to ensure the sustainability of its use in agriculture. This work considers the biochar's chemical-physical characterization and its potential phyto- and geno-toxicity, assessed with germination and Ames tests, obtaining valuable information for a safe field application. Three biochar types, obtained from gasification at different temperatures of green biomasses from the Tuscan-Emilian Apennines (in Italy), were compared through a broad chemical, physical and biological evaluation. The results obtained showed the relevance of temperature in determining the chemical and morphological properties of biochar, which was shown with several analytical techniques such as the elemental composition, water holding capacity, ash content, but also with FTIR and X-ray spectroscopies. These techniques showed the presence of different relevant surface aliphatic and aromatic groups. The procedures for evaluating the potential toxicity using seeds germination and Ames genotoxicity assay highlights that biochar does not cause detrimental effects when it enters in contact with soil, micro- and macro-organisms, and plants. The genotoxicity test provided a new highlight in evaluating biochar environmental safety.", "keywords": ["Gasification temperature", "0106 biological sciences", "Mutagenic assay", "Biochar risk assessment", "04 agricultural and veterinary sciences", "15. Life on land", "Chemical-physical tests", "Wood", "01 natural sciences", "630", "Environmental Management", "Soil", "Biochar Risk assessment Gasification temperature Chemical-physical tests Mutagenic assay Phytotoxicity", "13. 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As expected, the efficiency of ozonation was highly ozone dose- and contact time-dependent. The removal of the parent compounds of the selected antibiotics to levels below their detection limits was achieved with HRT of 40\u202fmin and specific ozone dose of 0.125 gO3 gDOC- 1. The effect of ozonation was also investigated at a microbiological and genomic level, by studying the efficiency of the process with respect to the inactivation of Escherichia coli and antibiotic-resistant E.\u00a0coli, as well as to the reduction of the abundance of selected antibiotic resistance genes (ARGs). The inactivation of total cultivable E.\u00a0coli was achieved under the experimental conditions of HRT 40\u202fmin and 0.25 gO3 gDOC-1, at which all antibiotic compounds were already degraded. The regrowth examinations revealed that higher ozone concentrations were required for the permanent inactivation of E.\u00a0coli below the Limit of Quantification (<LOQ\u202f=\u202f0.01\u202fCFU mL- 1). Also, the abundance of the examined ARGs (intl1, aadA1, dfrA1, qacE\u03941 and sul1) was found to decrease with increasing HRT and ozone dose. Despite the fact that the mildest operating parameters were able to eliminate the parent compounds of the tested antibiotics in wastewater effluents, it was clearly demonstrated in this study that higher ozone doses were required in order to confer permanent damage and/or death and prevent potential post-treatment re-growth of both total bacteria and ARB, and to reduce the abundance of ARGs below the LOQ. Interestingly, the mineralization of wastewater, in terms of Dissolved Organic Carbon (DOC) removal, was found to be significantly low even when the higher ozone doses were applied, leading to an increased phytotoxicity towards various plant species. The findings of this study clearly underline the importance of properly optimising the ozonation process (e.g. specific ozone dose and contact time) taking into consideration both the bacterial species and associated ARGs, as well as the wastewater physicochemical properties (e.g. DOC), in order to mitigate the spread of ARB&ARGs, as well as to reduce the potential phytotoxicity.", "keywords": ["Antibiotic resistance", "AntibioticsAntibiotic resistancePhytotoxicityOzonationContinuous mode", "0211 other engineering and technologies", "Drug Resistance", " Microbial", "02 engineering and technology", "Wastewater", "Waste Disposal", " Fluid", "01 natural sciences", "6. Clean water", "Continuous mode", "Anti-Bacterial Agents", "Water Purification", "3. Good health", "Ozone", "Antibiotics", "Ozonation", "Phytotoxicity", "11. 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We conducted a pilot-scale study to assess the potential conversion of A. dealbata biomass by vermicomposting via the earthworm Eisenia andrei. The flowering aerial A. dealbata biomass was shredded and placed in a vermireactor under greenhouse conditions for 56 days. The vermicomposted material was sampled every two weeks to analyse its biological and chemical parameters. The phytotoxicity of the fresh A. dealbata material and vermicompost was assessed via an ecotoxicological test with Lepidium sativum seeds. The activity of the earthworms caused strong modifications of the properties of the processed material: the electric conductivity, basal respiration, and organic matter content were reduced, whereas the concentrations of other elements such as N, P, or Zn increased. The earthworm biomass increased steadily until day 42 and then decreased, probably due to the depletion of labile organic matter during the initial stages of vermicomposting. The fresh A. dealbata material reduced the germination and radicle elongation of L. sativum, whereas vermicompost showed the same values as control. The produced vermicompost was an organic fertiliser rich in N and was not phytotoxic. Vermicomposting provides an opportunity to create a new value chain for the control of the invasive tree A. dealbata.</p></article>", "keywords": ["2401.91 Invertebrados no Insectos", "<i>Eisenia andrei</i>; epigeic earthworms; germination tests; organic fertiliser; phytotoxicity", "24 Ciencias de la Vida", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://www.mdpi.com/2071-1050/14/21/13828/pdf"}, {"href": "https://www.mdpi.com/2071-1050/14/21/13828/pdf"}, {"href": "https://doi.org/10.3390/su142113828"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/su142113828", "name": "item", "description": "10.3390/su142113828", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/su142113828"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-10-25T00:00:00Z"}}, {"id": "11381/2924969", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:27:11Z", "type": "Journal Article", "created": "2022-05-25", "title": "Building a risk matrix for the safety assessment of wood derived biochars", "description": "Biochar is recognized as an efficient amendment and soil improver. However, environmental and quality assessments are needed to ensure the sustainability of its use in agriculture. This work considers the biochar's chemical-physical characterization and its potential phyto- and geno-toxicity, assessed with germination and Ames tests, obtaining valuable information for a safe field application. Three biochar types, obtained from gasification at different temperatures of green biomasses from the Tuscan-Emilian Apennines (in Italy), were compared through a broad chemical, physical and biological evaluation. The results obtained showed the relevance of temperature in determining the chemical and morphological properties of biochar, which was shown with several analytical techniques such as the elemental composition, water holding capacity, ash content, but also with FTIR and X-ray spectroscopies. These techniques showed the presence of different relevant surface aliphatic and aromatic groups. The procedures for evaluating the potential toxicity using seeds germination and Ames genotoxicity assay highlights that biochar does not cause detrimental effects when it enters in contact with soil, micro- and macro-organisms, and plants. The genotoxicity test provided a new highlight in evaluating biochar environmental safety.", "keywords": ["Gasification temperature", "0106 biological sciences", "Mutagenic assay", "Biochar risk assessment", "04 agricultural and veterinary sciences", "15. Life on land", "Chemical-physical tests", "Wood", "01 natural sciences", "630", "Environmental Management", "Soil", "Biochar Risk assessment Gasification temperature Chemical-physical tests Mutagenic assay Phytotoxicity", "13. Climate action", "Phytotoxicity", "Charcoal", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Settore BIOS-10/A - Biologia cellulare e applicata", "Environmental Sciences"]}, "links": [{"href": "https://escholarship.org/content/qt0xs4h0ss/qt0xs4h0ss.pdf"}, {"href": "https://doi.org/11381/2924969"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11381/2924969", "name": "item", "description": "11381/2924969", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/2924969"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-01T00:00:00Z"}}, {"id": "11093/3991", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:27:06Z", "type": "Journal Article", "created": "2022-10-26", "title": "Vermicomposting as a Sustainable Option for Managing Biomass of the Invasive Tree Acacia dealbata Link", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>The tree Acacia dealbata is native to Australia but has become invasive in many parts of the world thanks to its N-fixing capacity and to the allelopathic compounds present in its biomass. We conducted a pilot-scale study to assess the potential conversion of A. dealbata biomass by vermicomposting via the earthworm Eisenia andrei. The flowering aerial A. dealbata biomass was shredded and placed in a vermireactor under greenhouse conditions for 56 days. The vermicomposted material was sampled every two weeks to analyse its biological and chemical parameters. The phytotoxicity of the fresh A. dealbata material and vermicompost was assessed via an ecotoxicological test with Lepidium sativum seeds. The activity of the earthworms caused strong modifications of the properties of the processed material: the electric conductivity, basal respiration, and organic matter content were reduced, whereas the concentrations of other elements such as N, P, or Zn increased. The earthworm biomass increased steadily until day 42 and then decreased, probably due to the depletion of labile organic matter during the initial stages of vermicomposting. The fresh A. dealbata material reduced the germination and radicle elongation of L. sativum, whereas vermicompost showed the same values as control. The produced vermicompost was an organic fertiliser rich in N and was not phytotoxic. Vermicomposting provides an opportunity to create a new value chain for the control of the invasive tree A. dealbata.</p></article>", "keywords": ["2401.91 Invertebrados no Insectos", "<i>Eisenia andrei</i>; epigeic earthworms; germination tests; organic fertiliser; phytotoxicity", "24 Ciencias de la Vida", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://www.mdpi.com/2071-1050/14/21/13828/pdf"}, {"href": "https://www.mdpi.com/2071-1050/14/21/13828/pdf"}, {"href": "https://doi.org/11093/3991"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11093/3991", "name": "item", "description": "11093/3991", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11093/3991"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-10-25T00:00:00Z"}}, {"id": "39847881", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:29:02Z", "type": "Journal Article", "created": "2024-09-10", "title": "Strategies of Physiological, Morpho-Anatomical and Biochemical Adaptation in Seedlings of Native Species Exposed to Mining Waste", "description": "Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100\u202f% contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10\u202f% to 25\u202f% of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) concentrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2\u00b7- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physicochemical characteristics for plant survival at highest contamination levels.", "keywords": ["Metal", "Soil pollution", "Germination", "Catalase", "Adaptation", " Physiological", "Environmental pollution", "Mining", "Phytoremediation", "Environmental sciences", "Soil", "Prosopis", "Ascorbate Peroxidases", "TD172-193.5", "Oxidative stress", "Seedlings", "Phytotoxicity", "Metals", " Heavy", "Seeds", "Arid environment", "Soil Pollutants", "GE1-350", "Peroxidase"]}, "links": [{"href": "https://doi.org/39847881"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecotoxicology%20and%20Environmental%20Safety", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "39847881", "name": "item", "description": "39847881", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/39847881"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phytotoxicity&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phytotoxicity&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phytotoxicity&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Phytotoxicity&offset=7", "hreflang": "en-US"}], "numberMatched": 7, "numberReturned": 7, "distributedFeatures": [], "timeStamp": "2026-04-04T13:38:04.352530Z"}