{"type": "FeatureCollection", "features": [{"id": "10.1016/j.cell.2021.04.024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:16:22Z", "type": "Journal Article", "created": "2021-05-18", "title": "Innovation, conservation, and repurposing of gene function in root cell type development", "description": "Plant species have evolved myriads of solutions, including complex cell type development and regulation, to adapt to dynamic environments. To understand this cellular diversity, we profiled tomato root cell type translatomes. Using xylem differentiation in tomato, examples of functional innovation, repurposing, and conservation of transcription factors are described, relative to the model plant Arabidopsis. Repurposing and innovation of genes are further observed within an exodermis regulatory network and illustrate its function. Comparative translatome analyses of rice, tomato, and Arabidopsis cell populations suggest increased expression conservation of root meristems compared with other homologous populations. In addition, the functions of constitutively expressed genes are more conserved than those of cell type/tissue-enriched genes. These observations suggest that higher order properties of cell type and pan-cell type regulation are evolutionarily conserved between plants and animals.", "keywords": ["root development", "translatomes", "General Biochemistry", "Genetics and Molecular Biology", "Green Fluorescent Proteins", "Meristem", "Arabidopsis", "cell types; evolution; exodermis; gene regulation; rice; root development; tomato; translatomes; xylem", "tomato", "xylem", "Genes", " Plant", "Plant Roots", "Inventions", "Solanum lycopersicum", "Species Specificity", "Gene Expression Regulation", " Plant", "Xylem", "evolution", "Gene Regulatory Networks", "Promoter Regions", " Genetic", "Plant Proteins", "2. Zero hunger", "exodermis", "rice", "15. Life on land", "Protein Biosynthesis", "cell types", "gene regulation", "Transcription Factors"]}, "links": [{"href": "https://www.research.unipd.it/bitstream/11577/3392826/2/PIIS0092867421005043.pdf"}, {"href": "https://doi.org/10.1016/j.cell.2021.04.024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Cell", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cell.2021.04.024", "name": "item", "description": "10.1016/j.cell.2021.04.024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cell.2021.04.024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2006.12.071", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-06-23T16:16:22Z", "type": "Journal Article", "created": "2007-02-21", "title": "The Copper Tolerance Strategies And The Role Of Antioxidative Enzymes In Three Plant Species Grown On Copper Mine", "description": "This study was undertaken to identify the strategies and the status of antioxidant enzyme activities involved in three plant species tolerance against Cu-toxicity in copper mine. The following methods were used for evaluations in three wild type species; Datura stramonium, Malva sylvestris and Chenopodium ambrosioides. The level of chlorophyll and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) by spectrometry, malondialdehyde (MDA) and dityrosine by HPLC and the levels of Cu in tissues and soils by atomic absorption spectrometry (AAS). Analysis showed that total and available copper were at toxic levels for plants growing on contaminated soil (zone 1). However, there were not any visual and conspicuous symptoms of Cu toxicity in plant species. Among three species, excess copper was transferred only into the D. stramonium and C. ambrosioides tissues. The C. ambrosioides accumulated Cu in roots and then in leaves, in which the leaves chloroplasts stored Cu around two times of vacuoles. In D. stramonium most of Cu was accumulated in leaves in which the storage rate in vacuoles and chloroplasts were 42% and 8%, respectively. In zone 1, the chlorophyll levels increased significantly in leaves of C. ambrosioides with respect to the same plant growing on uncontaminated soil (zone 2). There was insignificant decrease in chlorophyll content of D. stramonium leaves, collected from zone 1 with respect to zone 2. The D. stramonium and C. ambrosioides in zone 1, both revealed significant increase in their tissues antioxidant enzyme activities in comparison with the same samples of zone 2. There was significant elevation in oxidative damage biomarkers; MDA and dityrosine, when the aerial parts of D. stramonium in zone 1 were compared with the same parts of zone 2. We concluded that there were different tolerance strategies in studied plant species that protected them against copper toxicity. In M. sylvestris, exclusion of Cu from the roots or its stabilization in the soil restricted Cu toxicity effects. On the other hand D. stramonium and C. ambrosioides, elevated their antioxidative enzyme activities in response to cu-toxicity. In addition, the species D. stramonium accumulated excess of Cu in leaves vacuoles.", "keywords": ["Chlorophyll", "0106 biological sciences", "0301 basic medicine", "Chloroplasts", "Malva", "Plants", "15. Life on land", "Lipid Metabolism", "Plant Roots", "01 natural sciences", "Antioxidants", "Mining", "6. Clean water", "Chenopodium", "Oxidative Stress", "Soil", "03 medical and health sciences", "Malondialdehyde", "Vacuoles", "Datura", "Tyrosine", "Biomass", "Copper", "Plant Proteins"], "contacts": [{"organization": "Massod Mashhadi Akbar Boojar, Faranak Goodarzi,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2006.12.071"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2006.12.071", "name": "item", "description": "10.1016/j.chemosphere.2006.12.071", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2006.12.071"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-05-01T00:00:00Z"}}, {"id": "10.1016/j.foodres.2017.03.045", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:16:44Z", "type": "Journal Article", "created": "2018-08-22", "title": "Past, present and future: The strength of plant-based dairy substitutes based on gluten-free raw materials", "description": "As the pace of vaccine uptake accelerates globally, there is a need to document low-income country experiences with vaccine introductions. Over the course of five years, the government of Rwanda rolled out vaccines against pneumococcus, human papillomavirus, rotavirus, and measles & rubella, achieving over 90% coverage for each. To carry out these rollouts, Rwanda's Ministry of Health engaged in careful review of disease burden information and extensive, cross-sectoral planning at least one year before introducing each vaccine. Rwanda's local leaders, development partners, civil society organizations and widespread community health worker network were mobilized to support communication efforts. Community health workers were also used to confirm target population size. Support from Gavi, UNICEF and WHO was used in combination with government funds to promote country ownership and collaboration. Vaccination was also combined with additional community-based health interventions. Other countries considering rapid consecutive or simultaneous rollouts of new vaccines may consider lessons from Rwanda's experience while tailoring the strategies used to local context.", "keywords": ["Plant-based milk substitutes", "Measles Vaccine", "Plant Proteins", " Dietary", "Pneumococcal Vaccines", "Diet", " Gluten-Free", "03 medical and health sciences", "0404 agricultural biotechnology", "0302 clinical medicine", "Protein-high foods", "Food Quality", "Humans", "Rubella Vaccine", "Protein requirement", "Papillomavirus Vaccines", "Vaccines", " Combined", "Milk alternatives", "Plant-based dairy substitutes", "Food", " Formulated", "Community Health Workers", "Population Density", "2. Zero hunger", "Vaccines", "Immunization Programs", "Protein", "Diet", " Vegetarian", "Communication", "Vaccination", "Rotavirus Vaccines", "Rwanda", "1. No poverty", "04 agricultural and veterinary sciences", "Diets", "plant-based milk substitutes; protein requirement; milk alternatives; protein-high foods; protein; diets; plant-based dairy substitutes", "3. Good health", "Food Technology", "Dairy Products", "0405 other agricultural sciences", "Forecasting"], "contacts": [{"organization": "Gatera, Maurice, Bhatt, Sunil, Ngabo, Fidele, Utamuliza, Mathilde, Sibomana, Hassan, Karema, Corine, Mugeni, Cathy, Nutt, Cameron T., Nsanzimana, Sabin, Wagner, Claire M., Binagwaho, Agnes,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.foodres.2017.03.045"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Food%2C%20Nutrition%20and%20Population%20Health", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.foodres.2017.03.045", "name": "item", "description": "10.1016/j.foodres.2017.03.045", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.foodres.2017.03.045"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-06-01T00:00:00Z"}}, {"id": "10.1016/j.foodres.2018.01.020", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-06-23T16:16:44Z", "type": "Journal Article", "created": "2018-01-12", "title": "The impact of newly produced protein and dietary fiber rich fractions of yellow pea ( Pisum sativum L.) on the structure and mechanical properties of pasta-like sheets", "description": "Two fractions from pea (Pisum sativum L.), protein isolate (PPI) and dietary fiber (PF), were newly produced by extraction-fractionation method and characterized in terms of particle size distribution and structural morphology using SEM. The newly produced PPI and PF fractions were processed into pasta-like sheets with varying protein to fiber ratios (100/0, 90/10, 80/20, 70/30 and 50/50, respectively) using high temperature compression molding. We studied protein polymerization, molecular structure and protein-fiber interactions, as well as mechanical performance and cooking characteristics of processed PPI-PF blends. Bi-modal particle size distribution and chemical composition of the PPI and PF fractions influenced significantly the physicochemical properties of the pasta-like sheets. Polymerization was most pronounced for the 100 PPI, 90/10 and 80/20 PPI-PF samples as studied by SE-HPLC, and polymerization decreased with addition of the PF fraction. The mechanical properties, as strength and extensibility, were likewise the highest for the 100 PPI and 90/10 PPI-PF blends, while the E-modulus was similar for all the studied blends (around 38\u202fMPa). The extensibility decreased with the increasing amount of PF in the blend. The highest amounts of \u03b2-sheets were found in the pasta-like sheets with high amounts of PPI (100, 90 and 80%), by FT-IR. An increase in PF fraction in the blend, resulted into the high amounts of unordered structures as observed by FT-IR, as well as in an increase in the molecular scattering distances observed by SAXS. The water uptake increased and cooking loss decreased with increased proportions of the PF fraction, and the consistency of 10\u202fmin cooked pasta-like sheets were alike al dente texture. The new knowledge obtained in this study on the use of extraction-fractionation method to produce novel PPI and PF fractions for developing innovative high nutritious food can be of a great importance. The obtained knowledge on the pea protein and fiber processing behaviour could greatly contribute to a better control of functional properties of various temperature-processed products from yellow pea.", "keywords": ["Dietary Fiber", "2. Zero hunger", "Hot Temperature", "Food Handling", "Protein Conformation", "04 agricultural and veterinary sciences", "Plant Proteins", " Dietary", "Polymerization", "Structure-Activity Relationship", "0404 agricultural biotechnology", "Functional Food", "Elastic Modulus", "Scattering", " Small Angle", "Spectroscopy", " Fourier Transform Infrared", "Carbohydrate Conformation", "Chromatography", " Gel", "Dietary Carbohydrates", "Microscopy", " Electron", " Scanning", "Cooking", "Particle Size", "Nutritive Value", "Chromatography", " High Pressure Liquid", "Pisum sativum"]}, "links": [{"href": "https://doi.org/10.1016/j.foodres.2018.01.020"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Food%20Research%20International", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.foodres.2018.01.020", "name": "item", "description": "10.1016/j.foodres.2018.01.020", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.foodres.2018.01.020"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-04-01T00:00:00Z"}}, {"id": "10.1016/j.ijbiomac.2021.10.032", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:17:02Z", "type": "Journal Article", "created": "2021-10-14", "title": "Identification and molecular characterization of the high-affinity copper transporters family in Solanum lycopersicum.", "description": "Copper (Cu) plays a key role as cofactor in the plant proteins participating in essential cellular processes, such as electron transport and free radical scavenging. Despite high-affinity Cu transporters (COPTs) being key participants in Cu homeostasis maintenance, very little is known about COPTs in tomato (Solanum lycopersicum) even though it is the most consumed fruit worldwide and this crop is susceptible to suboptimal Cu conditions. In this study, a six-member family of COPT (SlCOPT1-6) was identified and characterized. SlCOPTs have a conserved architecture consisting of three transmembrane domains and \u03b2-strains. However, the presence of essential methionine residues, a methionine-enriched amino-terminal region, an Mx3Mx12Gx3G Cu-binding motif and a cysteine rich carboxy-terminal region, all required for their functionality, is more variable among members. Accordingly, functional complementation assays in yeast indicate that SlCOPT1 and SlCOPT2 are able to transport Cu inside the cell, while SlCOPT3 and SlCOPT5 are only partially functional. In addition, protein interaction network analyses reveal the connection between SlCOPTs and Cu PIB-type ATPases, other metal transporters, and proteins related to the peroxisome. Gene expression analyses uncover organ-dependency, fruit vasculature tissue specialization and ripening-dependent gene expression profiles, as well as different response to Cu deficiency or toxicity in an organ-dependent manner.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Biotecnologia agr\u00edcola", "Molecular Conformation", "COPT", "Gene Expression", "Tomato", "Structure-Activity Relationship", "03 medical and health sciences", "Copper Transport Proteins", "Solanum lycopersicum", "Multigene Family", "Tom\u00e0quets", "Amino Acid Sequence", "Heavy metal stress", "Conserved Sequence", "Copper", "Phylogeny", "Plant Proteins"]}, "links": [{"href": "https://doi.org/10.1016/j.ijbiomac.2021.10.032"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Biological%20Macromolecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijbiomac.2021.10.032", "name": "item", "description": "10.1016/j.ijbiomac.2021.10.032", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijbiomac.2021.10.032"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-01T00:00:00Z"}}, {"id": "10.1016/j.peptides.2021.170696", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:17:16Z", "type": "Journal Article", "created": "2021-11-29", "title": "Immunomodulatory peptides\u2014A promising source for novel functional food production and drug discovery", "description": "Immunomodulatory peptides are a complex class of bioactive peptides that encompasses substances with different mechanisms of action. Immunomodulatory peptides could also be used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics. Thus, immunomodulatory peptides in food of plant origin could be regarded both as valuable suplements of novel functional food preparation and/or as precursors or possible active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system. Given variety of mechanisms, different tests are required to assess effects of immunomodulatory peptides. Some of those effects show good correlation with in vivo results but others, less so. Certain plant peptides, such as defensins, show both immunomodulatory and antimicrobial effect, which makes them interesting candidates for preparation of functional food and feed, as well as templates for design of synthetic peptides.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Vaccines", "570", "0303 health sciences", "Immunomodulatory peptides  Defensin  Cryptic peptide  Functional food  Mechanism of action  Vaccines", "Functional food", "Cryptic peptide", "610", "Mechanism of action", "3. Good health", "Defensins", "Immunomodulation", "03 medical and health sciences", "Immunomodulatory peptide", "Defensin", "Functional Food", "Drug Discovery", "Humans", "Peptides", "Settore BIOS-10/A - Biologia cellulare e applicata", "Immunomodulatory peptides Defensin Cryptic peptide Functional food Mechanism of action Vaccines", "Plant Proteins"]}, "links": [{"href": "https://doi.org/10.1016/j.peptides.2021.170696"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Peptides", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.peptides.2021.170696", "name": "item", "description": "10.1016/j.peptides.2021.170696", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.peptides.2021.170696"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2008.11.046", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:17:20Z", "type": "Journal Article", "created": "2008-12-20", "title": "How Nitrogen And Sulphur Addition, And A Single Drought Event Affect Root Phosphatase Activity In Phalaris Arundinacea", "description": "Conservation and restoration of fens and fen meadows often aim to reduce soil nutrients, mainly nitrogen (N) and phosphorus (P). The biogeochemistry of P has received much attention as P-enrichment is expected to negatively impact on species diversity in wetlands. It is known that N, sulphur (S) and hydrological conditions affect the biogeochemistry of P, yet their interactive effects on P-dynamics are largely unknown. Additionally, in Europe, climate change has been predicted to lead to increases in summer drought. We performed a greenhouse experiment to elucidate the interactive effects of N, S and a single drought event on the P-availability for Phalaris arundinacea. Additionally, the response of plant phosphatase activity to these factors was measured over the two year experimental period. In contrast to results from earlier experiments, our treatments hardly affected soil P-availability. This may be explained by the higher pH in our soils, hampering the formation of Fe-P or Fe-Al complexes. Addition of S, however, decreased the plants N:P ratio, indicating an effect of S on the N:P stoichiometry and an effect on the plant's P-demand. Phosphatase activity increased significantly after addition of S, but was not affected by the addition of N or a single drought event. Root phosphatase activity was also positively related to plant tissue N and P concentrations, plant N and P uptake, and plant aboveground biomass, suggesting that the phosphatase enzyme influences P-biogeochemistry. Our results demonstrated that it is difficult to predict the effects of wetland restoration, since the involved mechanisms are not fully understood. Short-term and long-term effects on root phosphatase activity may differ considerably. Additionally, the addition of S can lead to unexpected effects on the biogeochemistry of P. Our results showed that natural resource managers should be careful when restoring degraded fens or preventing desiccation of fen ecosystems.", "keywords": ["summer", "0106 biological sciences", "plant tissue", "550", "Sulphate induced enzyme activity", "phosphorus limitation", "plant", "sulfate", "drought", "deposition", "Plant Roots", "01 natural sciences", "nitrogen", "iron", "biogeochemistry", "Root-surface phosphatase", "SDG 13 - Climate Action", "Phalaris", "species richness", "phosphorus", "N:P stoichiometry", "manager", "Plant Proteins", "2. Zero hunger", "pH", "grasslands", "Phosphorus", "dynamics", "04 agricultural and veterinary sciences", "wetland", "6. Clean water", "enzyme activity", "stoichiometry", "Europe", "eutrophication", "climate change", "Nitrogen", "growth", "fresh-water wetlands", "phosphatase", "soil", "desiccation", "Stress", " Physiological", "N:P ratios", "greenhouse", "N:P rations", "Fertilizers", "580", "Phosphorus uptake", "ecosystem", "biomass", "species diversity", "carbon", "nutrient", "15. Life on land", "Phosphoric Monoester Hydrolases", "enzyme", "fertilization", "13. Climate action", "Wetlands", "sulfur", "0401 agriculture", " forestry", " and fisheries", "Sulfur"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2008.11.046"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2008.11.046", "name": "item", "description": "10.1016/j.scitotenv.2008.11.046", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2008.11.046"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-03-01T00:00:00Z"}}, {"id": "10.1073/pnas.1913688117", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:46Z", "type": "Journal Article", "created": "2020-03-17", "title": "ENO regulates tomato fruit size through the floral meristem development network", "description": "<p>A dramatic evolution of fruit size has accompanied the domestication and improvement of fruit-bearing crop species. In tomato (Solanum lycopersicum), naturally occurring cis-regulatory mutations in the genes of the CLAVATA-WUSCHEL signaling pathway have led to a significant increase in fruit size generating enlarged meristems that lead to flowers with extra organs and bigger fruits. In this work, by combining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolatedEXCESSIVE NUMBER OF FLORAL ORGANS(ENO), an AP2/ERF transcription factor which regulates floral meristem activity. Thus, theENOgene mutation gives rise to plants that yield larger multilocular fruits due to an increased size of the floral meristem. Genetic analyses indicate thatenoexhibits synergistic effects with mutations at theLOCULE NUMBER(encodingSlWUS) andFASCIATED(encodingSlCLV3) loci, two central players in the evolution of fruit size in the domestication of cultivated tomatoes. Our findings reveal that anenomutation causes a substantial expansion ofSlWUSexpression domains in a flower-specific manner. In vitro binding results show that ENO is able to interact with the GGC-box cis-regulatory element within theSlWUSpromoter region, suggesting that ENO directly regulatesSlWUSexpression domains to maintain floral stem-cell homeostasis. Furthermore, the study of natural allelic variation of theENOlocus proved that a cis-regulatory mutation in the promoter ofENOhad been targeted by positive selection during the domestication process, setting up the background for significant increases in fruit locule number and fruit size in modern tomatoes.</p>", "keywords": ["0301 basic medicine", "570", "Floral meristem", "[SPI] Engineering Sciences [physics]", "[SDV]Life Sciences [q-bio]", "Meristem", "Quantitative Trait Loci", "Genes", " Plant", "CLAVATA/WUSCHEL regulatory network", "Domestication", "[SPI]Engineering Sciences [physics]", "03 medical and health sciences", "Solanum lycopersicum", "Gene Expression Regulation", " Plant", "AP2/ERF transcription factor", "Promoter Regions", " Genetic", "Cell Proliferation", "Plant Proteins", "580", "Homeodomain Proteins", "2. Zero hunger", "Tomato (Solanum lycopersicum)", "0303 health sciences", "Stem Cells", "Biological Sciences", "15. Life on land", "fruit size", "Crop Production", "[SDV] Life Sciences [q-bio]", "CLAVATA-WUSCHEL regulatory network", "GENETICA", "Fruit", "Mutation", "Fruit size", "floral meristem", "Transcription Factors"]}, "links": [{"href": "https://pnas.org/doi/pdf/10.1073/pnas.1913688117"}, {"href": "https://doi.org/10.1073/pnas.1913688117"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.1913688117", "name": "item", "description": "10.1073/pnas.1913688117", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.1913688117"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-16T00:00:00Z"}}, {"id": "10.1038/s41586-024-07607-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:26Z", "type": "Journal Article", "created": "2024-06-26", "title": "Zinc mediates control of nitrogen fixation via transcription factor filamentation", "description": "Abstract<p>Plants adapt to fluctuating environmental conditions by adjusting their metabolism and gene expression to maintain fitness1. In legumes, nitrogen homeostasis is maintained by balancing nitrogen acquired from soil resources with nitrogen fixation by symbiotic bacteria in root nodules2\uffe2\uff80\uff938. Here we show that zinc, an essential plant micronutrient, acts as an intracellular second messenger that connects environmental changes to transcription factor control of metabolic activity in root nodules. We identify a transcriptional regulator, FIXATION UNDER NITRATE (FUN), which acts as a sensor, with zinc controlling the transition between an inactive filamentous megastructure and an active transcriptional regulator. Lower zinc concentrations in the nodule, which we show occur in response to higher levels of soil nitrate, dissociates the filament and activates FUN. FUN then directly targets multiple pathways to initiate breakdown of the nodule. The zinc-dependent filamentation mechanism thus establishes a concentration readout to adapt nodule function to the environmental nitrogen conditions. In a wider perspective, these results have implications for understanding the roles of metal ions in integration of environmental signals with plant development and optimizing delivery of fixed nitrogen in legume crops.</p", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Nitrates", "Nitrogen", "Second Messenger Systems", "Article", "Zinc", "03 medical and health sciences", "Plant signalling", "Gene Expression Regulation", " Plant", "Nitrogen Fixation", "Lotus", "Root Nodules", " Plant", "Symbiosis", "Rhizobial symbiosis", "Plant Proteins", "Transcription Factors"]}, "links": [{"href": "https://doi.org/10.1038/s41586-024-07607-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41586-024-07607-6", "name": "item", "description": "10.1038/s41586-024-07607-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41586-024-07607-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-06-26T00:00:00Z"}}, {"id": "10.1038/s41467-019-14197-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:23Z", "type": "Journal Article", "created": "2020-01-24", "title": "High-quality genome sequence of white lupin provides insight into soil exploration and seed quality", "description": "Abstract<p>White lupin (Lupinus albus L.) is an annual crop cultivated for its protein-rich seeds. It is adapted to poor soils due to the production of cluster roots, which are made of dozens of determinate lateral roots that drastically improve soil exploration and nutrient acquisition (mostly phosphate). Using long-read sequencing technologies, we provide a high-quality genome sequence of a cultivated accession of white lupin (2n\uffe2\uff80\uff89=\uffe2\uff80\uff8950, 451\uffe2\uff80\uff89Mb), as well as de novo assemblies of a landrace and a wild relative. We describe a modern accession displaying increased soil exploration capacity through early establishment of lateral and cluster roots. We also show how seed quality may have been impacted by domestication in term of protein profiles and alkaloid content. The availability of a high-quality genome assembly together with companion genomic and transcriptomic resources will enable the development of modern breeding strategies to increase and stabilize white lupin yield.</p>", "keywords": ["Repetitive Sequences", " Nucleic Acid/genetics", "0301 basic medicine", "[SDV]Life Sciences [q-bio]", "Plant Roots/genetics", "Gene Dosage", "Plant Science", "Crop", "Alkaloids/chemistry", "Plant Roots", "Gene", "Repetitive Sequences", "630", "Agricultural and Biological Sciences", "Domestication", "Soil", "Models", "Symbiotic Nitrogen Fixation in Legumes", "Gene Duplication", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "http://aims.fao.org/aos/agrovoc/c_3224", "Plant Proteins/metabolism", "Plant Proteins", "2. Zero hunger", "0303 health sciences", "Genome", "Q", "http://aims.fao.org/aos/agrovoc/c_27583", "Life Sciences", "Transcriptome/genetics", "http://aims.fao.org/aos/agrovoc/c_92382", "Polymorphism", " Single Nucleotide/genetics", "Lupinus", "[SDV] Life Sciences [q-bio]", "Protein Crop", "Seeds", "http://aims.fao.org/aos/agrovoc/c_5956", "White (mutation)", "Single Nucleotide/genetics", "Sequence Analysis", "Genome", " Plant", "expression des g\u00e8nes", "http://aims.fao.org/aos/agrovoc/c_4464", "Synteny/genetics", "Evolution", "Lupin Seeds", "Science", "Centromere", "Lupinus/genetics", "Polymorphism", " Single Nucleotide", "Article", "g\u00e9nomique", "Evolution", " Molecular", "Evolution and Nutritional Properties of Lupin Seeds", "physiologie v\u00e9g\u00e9tale", "03 medical and health sciences", "Alkaloids", "Genetic", "Nucleic Acid/genetics", "Seeds/physiology", "Centromere/genetics", "Genetics", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Polymorphism", "Biology", "Ecology", " Evolution", " Behavior and Systematics", "Repetitive Sequences", " Nucleic Acid", "Sequence assembly", "http://aims.fao.org/aos/agrovoc/c_25189", "Ecotype", "Models", " Genetic", "g\u00e9nome", "Botany", "Molecular", "Genetic Variation", "Molecular Sequence Annotation", "Plant", "DNA", "Sequence Analysis", " DNA", "s\u00e9quence nucl\u00e9otidique", "15. Life on land", "http://aims.fao.org/aos/agrovoc/c_27527", "Agronomy", "Plant Leaves", "Evolution and Ecology of Endophyte-Grass Symbiosis", "Lupinus albus", "FOS: Biological sciences", "Genomic Structural Variation", "Plant Leaves/metabolism", "Gene expression", "Transcriptome", "am\u00e9lioration des plantes"]}, "links": [{"href": "https://www.nature.com/articles/s41467-019-14197-9.pdf"}, {"href": "https://doi.org/10.1038/s41467-019-14197-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-019-14197-9", "name": "item", "description": "10.1038/s41467-019-14197-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-019-14197-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-24T00:00:00Z"}}, {"id": "10.1038/s41467-023-42911-1", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:18:24Z", "type": "Journal Article", "created": "2023-11-07", "title": "Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus", "description": "Abstract<p>Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads progress to cortical layers to establish functional nodules. Here we use single-cell analysis to define the epidermal and cortical cell populations that respond to and facilitate rhizobium infection. We then identify high-confidence nodulation gene candidates based on their specific expression in these populations, pinpointing genes stably associated with infection across genotypes and time points. We show that one of these, which we name SYMRKL1, encodes a protein with an ectodomain predicted to be nearly identical to that of SYMRK and is required for normal infection thread formation. Our work disentangles cellular processes and transcriptional modules that were previously confounded due to lack of cellular resolution, providing a more detailed understanding of symbiotic interactions.</p", "keywords": ["Rhizobium/metabolism", "Science", "Q", "Plant Roots/metabolism", "Plant Proteins/genetics", "Plant Roots", "Article", "Root Nodules", " Plant/metabolism", "Symbiosis/genetics", "Phenotype", "Lotus/metabolism", "Gene Expression Regulation", " Plant", "Lotus", "Single-Cell Analysis", "Root Nodules", " Plant", "Symbiosis", "Rhizobium", "Plant Proteins"]}, "links": [{"href": "https://www.nature.com/articles/s41467-023-42911-1.pdf"}, {"href": "https://doi.org/10.1038/s41467-023-42911-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-023-42911-1", "name": "item", "description": "10.1038/s41467-023-42911-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-023-42911-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-11-07T00:00:00Z"}}, {"id": "10.1038/s41586-023-05791-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:26Z", "type": "Journal Article", "created": "2023-03-08", "title": "The giant diploid faba genome unlocks variation in a global protein crop", "description": "Abstract<p>Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity1. However, plant protein production is hampered by the lack of a cool-season legume equivalent to soybean in agronomic value2. Faba bean (Vicia fabaL.) has a high yield potential and is well suited for cultivation in temperate regions, but genomic resources are scarce. Here, we report a high-quality chromosome-scale assembly of the faba bean genome and show that it has expanded to a massive 13\uffe2\uff80\uff89Gb in size through an imbalance between the rates of amplification and elimination of retrotransposons and satellite repeats. Genes and recombination events are evenly dispersed across chromosomes and the gene space is remarkably compact considering the genome size, although with substantial copy number variation driven by tandem duplication. Demonstrating practical application of the genome sequence, we develop a targeted genotyping assay and use high-resolution genome-wide association analysis to dissect the genetic basis of seed size and hilum colour. The resources presented constitute a genomics-based breeding platform for faba bean, enabling breeders and geneticists to accelerate the\uffc2\uffa0improvement of sustainable protein production across the\uffc2\uffa0Mediterranean, subtropical and northern temperate agroecological zones.</p", "keywords": ["Crops", " Agricultural", "DNA Copy Number Variations", "Retroelements", "[SDV]Life Sciences [q-bio]", "DNA", " Satellite", "Genes", " Plant", "630", "Article", "Chromosomes", " Plant", "Plant Proteins", "Recombination", " Genetic", "2. Zero hunger", "Geography", "Gene Amplification", "Genetic Variation", "Genomics", "15. Life on land", "11831 Plant biology", "Diploidy", "Agronomy", "metabolism ; Genome-Wide Association Study ; Plant Proteins ; genetics ; Plant Breeding ; Vicia faba ; DNA Copy Number Variations ; Diploidy", "Vicia faba", "[SDV] Life Sciences [q-bio]", "Plant Breeding", "Genetics", " developmental biology", " physiology", "13. Climate action", "Seeds", "Genome", " Plant", "info:eu-repo/classification/ddc/500", "Genome-Wide Association Study"]}, "links": [{"href": "https://doi.org/10.1038/s41586-023-05791-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41586-023-05791-5", "name": "item", "description": "10.1038/s41586-023-05791-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41586-023-05791-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-26T00:00:00Z"}}, {"id": "10.1038/s41598-019-51204-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:27Z", "type": "Journal Article", "created": "2019-10-16", "title": "Phosphorus-acquisition strategies of canola, wheat and barley in soil amended with sewage sludges", "description": "Abstract<p>Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition strategies of three main crops are affected by the application of sewage sludges, compared with a mineral P fertilizer. We carried out a 3-months greenhouse pot experiment and compared the response of P-acquisition traits among wheat, barley and canola in a soil amended with three sludges or a mineral P fertilizer. Results showed that the P-acquisition strategy differed among crops. Compared with canola, wheat and barley had a higher specific root length and a greater root carboxylate release and they acquired as much P from sludge as from mineral P. By contrast, canola shoot P content was greater with sludge than with mineral P. This was attributed to a higher root-released acid phosphatase activity which promoted the mineralization of sludge-derived P-organic. This study showed that contrasted P-acquisition strategies of crops allows increased use of renewable P resources by optimizing combinations of crop and the type of P fertilizer applied within the cropping system.</p", "keywords": ["Calcium Phosphates", "Crops", " Agricultural", "0106 biological sciences", "phosphatase activity", "N\u00e4hrstoffaufnahme", "carboxylate", "Phytic Acid", "Acid Phosphatase", "[SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy", "Carboxylic Acids", "organic P fertilizer", " mineral P fertilizer", " carboxylate", " phosphatase activity", "Plant Roots", "01 natural sciences", "630", "Article", "12. Responsible consumption", "Soil", "Boden", "Species Specificity", "ddc:630", "Humans", "Fertilizers", "Triticum", "Plant Proteins", "2. Zero hunger", "Plant Stems", "Sewage", "Brassica rapa", "Agriculture", "Biological Transport", "Hordeum", "Phosphorus", "Phosphor", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "0401 agriculture", " forestry", " and fisheries", "G\u00e4rrest", "mineral P fertilizer", "organic P fertilizer"]}, "links": [{"href": "https://www.nature.com/articles/s41598-019-51204-x.pdf"}, {"href": "https://doi.org/10.1038/s41598-019-51204-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-019-51204-x", "name": "item", "description": "10.1038/s41598-019-51204-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-019-51204-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-16T00:00:00Z"}}, {"id": "10.1039/c7fo00415j", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:31Z", "type": "Journal Article", "created": "2017-06-19", "title": "Correlation analysis of protein quality characteristics with gluten-free bread properties", "description": "<p>Research demonstrates the correlation of protein technofunctional properties with gluten-free bread quality characteristics.</p>", "keywords": ["2. Zero hunger", "0404 agricultural biotechnology", "Glutens", "Flour", "Food Quality", "Bread", "Cooking", "04 agricultural and veterinary sciences", "Rheology", "0405 other agricultural sciences", "Plant Proteins"]}, "links": [{"href": "http://pubs.rsc.org/en/content/articlepdf/2017/FO/C7FO00415J"}, {"href": "https://doi.org/10.1039/c7fo00415j"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Food%20%26amp%3B%20Function", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1039/c7fo00415j", "name": "item", "description": "10.1039/c7fo00415j", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1039/c7fo00415j"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-01T00:00:00Z"}}, {"id": "10.1073/pnas.2201072119", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:46Z", "type": "Journal Article", "created": "2022-07-18", "title": "Ethylene inhibits rice root elongation in compacted soil via ABA- and auxin-mediated mechanisms", "description": "<p>             Soil compaction represents a major agronomic challenge, inhibiting root elongation and impacting crop yields. Roots use ethylene to sense soil compaction as the restricted air space causes this gaseous signal to accumulate around root tips. Ethylene inhibits root elongation and promotes radial expansion in compacted soil, but its mechanistic basis remains unclear. Here, we report that ethylene promotes abscisic acid (ABA) biosynthesis and cortical cell radial expansion. Rice mutants of ABA biosynthetic genes had attenuated cortical cell radial expansion in compacted soil, leading to better penetration. Soil compaction-induced ethylene also up-regulates the auxin biosynthesis gene             OsYUC8             . Mutants lacking OsYUC8 are better able to penetrate compacted soil. The auxin influx transporter OsAUX1 is also required to mobilize auxin from the root tip to the elongation zone during a root compaction response. Moreover,             osaux1             mutants penetrate compacted soil better than the wild-type roots and do not exhibit cortical cell radial expansion. We conclude that ethylene uses auxin and ABA as downstream signals to modify rice root cell elongation and radial expansion, causing root tips to swell and reducing their ability to penetrate compacted soil.           </p", "keywords": ["roots", "0301 basic medicine", "570", "Cell biology", "Arabidopsis", "Biophysics", "Plant Science", "Plant Roots", "Biochemistry", "Gene", "Catalysis", "Mixed Function Oxygenases", "Molecular Mechanisms of Plant Development and Regulation", "soil compaction", "Agricultural and Biological Sciences", "Soil", "Abscisic acid", "Ethylene", "03 medical and health sciences", "aba", "ethylene", "Auxin", "Elongation", "Biology", "Plant Proteins", "580", "2. Zero hunger", "0303 health sciences", "Multidisciplinary", "Indoleacetic Acids", "Mutant", "Life Sciences", "Oryza", "Plant Nutrient Uptake and Signaling Pathways", "Biological Sciences", "Ethylenes", "15. Life on land", "Materials science", "Root Aeration", "Chemistry", "ABA", "Plant Responses to Flooding Stress", "Ultimate tensile strength", "Mutation", "Metallurgy", "auxin", "Abscisic Acid"]}, "links": [{"href": "https://pnas.org/doi/pdf/10.1073/pnas.2201072119"}, {"href": "https://doi.org/10.1073/pnas.2201072119"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.2201072119", "name": "item", "description": "10.1073/pnas.2201072119", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.2201072119"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-18T00:00:00Z"}}, {"id": "10.1093/jxb/erq249", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:05Z", "type": "Journal Article", "created": "2010-08-27", "title": "Plant Physiology And Proteomics Reveals The Leaf Response To Drought In Alfalfa (Medicago Sativa L.)", "description": "Despite its relevance, protein regulation, metabolic adjustment, and the physiological status of plants under drought is not well understood in relation to the role of nitrogen fixation in nodules. In this study, nodulated alfalfa plants were exposed to drought conditions. The study determined the physiological, metabolic, and proteomic processes involved in photosynthetic inhibition in relation to the decrease in nitrogenase (N(ase)) activity. The deleterious effect of drought on alfalfa performance was targeted towards photosynthesis and N(ase) activity. At the leaf level, photosynthetic inhibition was mainly caused by the inhibition of Rubisco. The proteomic profile and physiological measurements revealed that the reduced carboxylation capacity of droughted plants was related to limitations in Rubisco protein content, activation state, and RuBP regeneration. Drought also decreased amino acid content such as asparagine, and glutamic acid, and Rubisco protein content indicating that N availability limitations were caused by N(ase) activity inhibition. In this context, drought induced the decrease in Rubisco binding protein content at the leaf level and proteases were up-regulated so as to degrade Rubisco protein. This degradation enabled the reallocation of the Rubisco-derived N to the synthesis of amino acids with osmoregulant capacity. Rubisco degradation under drought conditions was induced so as to remobilize Rubisco-derived N to compensate for the decrease in N associated with N(ase) inhibition. Metabolic analyses showed that droughted plants increased amino acid (proline, a major compound involved in osmotic regulation) and soluble sugar (D-pinitol) levels to contribute towards the decrease in osmotic potential (\u03a8(s)). At the nodule level, drought had an inhibitory effect on N(ase) activity. This decrease in N(ase) activity was not induced by substrate shortage, as reflected by an increase in total soluble sugars (TSS) in the nodules. Proline accumulation in the nodule could also be associated with an osmoregulatory response to drought and might function as a protective agent against ROS. In droughted nodules, the decrease in N(2) fixation was caused by an increase in oxygen resistance that was induced in the nodule. This was a mechanism to avoid oxidative damage associated with reduced respiration activity and the consequent increase in oxygen content. This study highlighted that even though drought had a direct effect on leaves, the deleterious effects of drought on nodules also conditioned leaf responsiveness.", "keywords": ["Proteomics", "0301 basic medicine", "570", "Rubisco", "Proteome", "[SDV]Life Sciences [q-bio]", "proteome", "N-2 FIXATION RESPONSE", "drought", "N2 fixation", "03 medical and health sciences", "XANTHOPHYLL CYCLE", "N-2 fixation", "2-CYSTEINE PEROXIREDOXIN", "Nitrogenase", "oxidative stress", "AMINO-ACIDS", "Photosynthesis", "climate", "agriculture", "Plant Proteins", "580", "N remobilization", "2. Zero hunger", "0303 health sciences", "photosynthesis", "PINITOL ACCUMULATION", "Drought", "RIBULOSE-1", "5-BISPHOSPHATE CARBOXYLASE-OXYGENASE", "Water", "BRASSICA-NAPUS", "N(O)-TERT-BUTYLDIMETHYLSILYL DERIVATIVES", "15. Life on land", "Research Papers", "6. Clean water", "Droughts", "[SDV] Life Sciences [q-bio]", "Plant Leaves", "nitrogen fixation", "Oxidative stress", "rubisco", "NITROGEN-FIXATION", "WATER-WATER CYCLE", "Medicago sativa"]}, "links": [{"href": "https://doi.org/10.1093/jxb/erq249"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jxb/erq249", "name": "item", "description": "10.1093/jxb/erq249", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jxb/erq249"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-25T00:00:00Z"}}, {"id": "10.1093/plcell/koae135", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:06Z", "type": "Journal Article", "created": "2024-05-01", "title": "Arabinosylation of cell wall extensin is required for the directional response to salinity in roots", "description": "Abstract                <p>Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity.</p", "keywords": ["580", "0301 basic medicine", "2. Zero hunger", "570", "Salinity", "0303 health sciences", "Glycosylation", "Arabidopsis Proteins", "Arabidopsis", "Breakthrough Report", "Sodium Chloride", "15. Life on land", "Plant Roots", "Arabinose", "Gravitropism", "03 medical and health sciences", "Cell Wall", "Gene Expression Regulation", " Plant", "Life Science", "Glycoproteins", "Plant Proteins"]}, "links": [{"href": "https://academic.oup.com/plcell/article-pdf/36/9/3328/59006321/koae135.pdf"}, {"href": "https://doi.org/10.1093/plcell/koae135"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Plant%20Cell", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/plcell/koae135", "name": "item", "description": "10.1093/plcell/koae135", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/plcell/koae135"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-01T00:00:00Z"}}, {"id": "10.1093/plcell/koae201", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:06Z", "type": "Journal Article", "created": "2024-07-16", "title": "Gibberellin dynamics governing nodulation revealed using GIBBERELLIN PERCEPTION SENSOR 2 in Medicago truncatula lateral organs", "description": "Abstract                <p>During nutrient scarcity, plants can adapt their developmental strategy to maximize their chance of survival. Such plasticity in development is underpinned by hormonal regulation, which mediates the relationship between environmental cues and developmental outputs. In legumes, endosymbiosis with nitrogen-fixing bacteria (rhizobia) is a key adaptation for supplying the plant with nitrogen in the form of ammonium. Rhizobia are housed in lateral root-derived organs termed nodules that maintain an environment conducive to Nitrogenase in these bacteria. Several phytohormones are important for regulating the formation of nodules, with both positive and negative roles proposed for gibberellin (GA). In this study, we determine the cellular location and function of bioactive GA during nodule organogenesis using a genetically encoded second-generation GA biosensor, GIBBERELLIN PERCEPTION SENSOR 2 in Medicago truncatula. We find endogenous bioactive GA accumulates locally at the site of nodule primordia, increasing dramatically in the cortical cell layers, persisting through cell divisions, and maintaining accumulation in the mature nodule meristem. We show, through misexpression of GA-catabolic enzymes that suppress GA accumulation, that GA acts as a positive regulator of nodule growth and development. Furthermore, increasing or decreasing GA through perturbation of biosynthesis gene expression can increase or decrease the size of nodules, respectively. This is unique from lateral root formation, a developmental program that shares common organogenesis regulators. We link GA to a wider gene regulatory program by showing that nodule-identity genes induce and sustain GA accumulation necessary for proper nodule formation.</p", "keywords": ["2. Zero hunger", "Plant Growth Regulators", "Gene Expression Regulation", " Plant", "Medicago truncatula", "Root Nodules", " Plant", "Plants", " Genetically Modified", "Plant Root Nodulation", "Plant Roots", "Gibberellins", "Research Article", "Plant Proteins"]}, "links": [{"href": "https://doi.org/10.1093/plcell/koae201"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Plant%20Cell", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/plcell/koae201", "name": "item", "description": "10.1093/plcell/koae201", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/plcell/koae201"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-07-16T00:00:00Z"}}, {"id": "10.1093/plphys/kiaa049", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:06Z", "type": "Journal Article", "created": "2020-05-31", "title": "Distinct signaling routes mediate intercellular and intracellular rhizobial infection in Lotus japonicus", "description": "Abstract<p>Rhizobial infection of legume roots during development of nitrogen fixing root nodules occurs either intracellularly though plant derived infection threads traversing the epidermal and cortical cell layers to deliver the bacteria or intercellularly via bacterial entry between epidermal plant cells. Although, around 25% of all legume genera are postulated to be intercellularly infected, the pathways and mechanisms supporting this process has remained virtually unexplored due to lack of genetically amenable legumes that have this infection mode. In this study, we report that the model legume Lotus japonicus is infected intercellularly by Rhizobium sp. IRBG74 and demonstrate that the resources available in Lotus enable insight into the genetic requirements and the fine-tuning of the pathway governing intercellular infection. Inoculation of Lotus mutants shows that Ern1 and RinRK1 are dispensable for intercellular infection in contrast to intracellular infection. Other symbiotic genes, including Nfr5, SymRK, CCaMK, Epr3, Cyclops, Nin, Nsp1, Nsp2, Cbs and Vpy1 are equally important for both entry modes. Comparative RNAseq analysis of roots inoculated with IRBG74 revealed a distinctive transcriptome response compared to intracellular colonization. In particular, a number of cytokinin-related genes were differentially regulated. Corroborating this observation cyp735A and ipt4 cytokinin biosynthesis mutants were significantly affected in their nodulation with IRBG74 while lhk1 cytokinin receptor mutants did not form any nodules. These results indicate that a differential requirement for cytokinin signalling conditions intercellular rhizobial entry and highlight the distinct modalities of the inter- and intra-cellular infection mechanisms.</p>", "keywords": ["name=Physiology", "580", "0301 basic medicine", "/dk/atira/pure/subjectarea/asjc/1300/1314", "0303 health sciences", "/dk/atira/pure/subjectarea/asjc/1300/1311", "571", "Regular Issue", "name=Genetics", "/dk/atira/pure/subjectarea/asjc/1100/1110", "Plant Roots", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Calcium-Calmodulin-Dependent Protein Kinases", "Lotus", "name=Plant Science", "Root Nodules", " Plant", "Plant Proteins", "Rhizobium", "Signal Transduction"]}, "links": [{"href": "http://academic.oup.com/plphys/article-pdf/185/3/1131/37166752/kiaa049.pdf"}, {"href": "https://doi.org/10.1093/plphys/kiaa049"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/plphys/kiaa049", "name": "item", "description": "10.1093/plphys/kiaa049", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/plphys/kiaa049"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-31T00:00:00Z"}}, {"id": "10.1093/plphys/kiae458", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:06Z", "type": "Journal Article", "created": "2024-08-28", "title": "Leaf Gas Film 1 promotes glycerol ester accumulation and formation of a tight root barrier to radial O2 loss in rice", "description": "Abstract                <p>Rice (Oryza sativa L.) and many other wetland plants form an apoplastic barrier in the outer parts of the roots to restrict radial O2 loss to the rhizosphere during soil flooding. This barrier facilitates longitudinal internal O2 diffusion via gas-filled tissues from shoot to root apices, enabling root growth in anoxic soils. We tested the hypothesis that Leaf Gas Film 1 (LGF1), which influences leaf hydrophobicity in rice, plays a crucial role in tight outer apoplastic barrier formation in rice roots. We examined the roots of a rice mutant (dripping wet leaf 7, drp7) lacking functional LGF1, its wild type, and an LGF1 overexpression line for their capacity to develop outer apoplastic barriers that restrict radial O2 loss. We quantified the chemical composition of the outer part of the root and measured radial O2 diffusion from intact roots. The drp7 mutant exhibited a weak barrier to radial O2 loss compared to the wild type. However, introducing functional LGF1 into the mutant fully restored tight barrier function. The formation of a tight barrier to radial O2 loss was associated with increased glycerol ester levels in exodermal cells, rather than differences in total root suberization or lignification. These results demonstrate that, in addition to its role in leaf hydrophobicity regulation, LGF1 plays an important role in controlling the function of the outer apoplastic barriers in roots. Our study suggests that increased deposition of glycerol esters in the suberized root exodermis establishes a tight barrier to radial O2 loss in rice roots.</p", "keywords": ["Oxygen", "Glycerol", "Plant Leaves", "2. Zero hunger", "0106 biological sciences", "Mutation", "Oryza", "Esters", "15. Life on land", "Plant Roots", "01 natural sciences", "Research Article", "Plant Proteins"]}, "links": [{"href": "https://academic.oup.com/plphys/article-pdf/196/4/2437/60933328/kiae458.pdf"}, {"href": "https://doi.org/10.1093/plphys/kiae458"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/plphys/kiae458", "name": "item", "description": "10.1093/plphys/kiae458", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/plphys/kiae458"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-28T00:00:00Z"}}, {"id": "10.1101/2020.05.29.124313", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:11Z", "type": "Journal Article", "created": "2020-05-31", "title": "Distinct signalling routes mediates intercellular and intracellular rhizobial infection in Lotus japonicus", "description": "Abstract<p>Rhizobial infection of legume roots during development of nitrogen fixing root nodules occurs either intracellularly though plant derived infection threads traversing the epidermal and cortical cell layers to deliver the bacteria or intercellularly via bacterial entry between epidermal plant cells. Although, around 25% of all legume genera are postulated to be intercellularly infected, the pathways and mechanisms supporting this process has remained virtually unexplored due to lack of genetically amenable legumes that have this infection mode. In this study, we report that the model legume Lotus japonicus is infected intercellularly by Rhizobium sp. IRBG74 and demonstrate that the resources available in Lotus enable insight into the genetic requirements and the fine-tuning of the pathway governing intercellular infection. Inoculation of Lotus mutants shows that Ern1 and RinRK1 are dispensable for intercellular infection in contrast to intracellular infection. Other symbiotic genes, including Nfr5, SymRK, CCaMK, Epr3, Cyclops, Nin, Nsp1, Nsp2, Cbs and Vpy1 are equally important for both entry modes. Comparative RNAseq analysis of roots inoculated with IRBG74 revealed a distinctive transcriptome response compared to intracellular colonization. In particular, a number of cytokinin-related genes were differentially regulated. Corroborating this observation cyp735A and ipt4 cytokinin biosynthesis mutants were significantly affected in their nodulation with IRBG74 while lhk1 cytokinin receptor mutants did not form any nodules. These results indicate that a differential requirement for cytokinin signalling conditions intercellular rhizobial entry and highlight the distinct modalities of the inter- and intra-cellular infection mechanisms.</p", "keywords": ["name=Physiology", "580", "0301 basic medicine", "/dk/atira/pure/subjectarea/asjc/1300/1314", "0303 health sciences", "/dk/atira/pure/subjectarea/asjc/1300/1311", "571", "Regular Issue", "name=Genetics", "/dk/atira/pure/subjectarea/asjc/1100/1110", "Plant Roots", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Calcium-Calmodulin-Dependent Protein Kinases", "Lotus", "name=Plant Science", "Root Nodules", " Plant", "Plant Proteins", "Rhizobium", "Signal Transduction"]}, "links": [{"href": "http://academic.oup.com/plphys/article-pdf/185/3/1131/37166752/kiaa049.pdf"}, {"href": "https://doi.org/10.1101/2020.05.29.124313"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1101/2020.05.29.124313", "name": "item", "description": "10.1101/2020.05.29.124313", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1101/2020.05.29.124313"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-31T00:00:00Z"}}, {"id": "10.1104/pp.19.00818", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:14Z", "type": "Journal Article", "created": "2019-09-30", "title": "SnRK2 Protein Kinases and mRNA Decapping Machinery Control Root Development and Response to Salt", "description": "SNF1-RELATED PROTEIN KINASES 2 (SnRK2) are important components of early osmotic and salt stress signaling pathways in plants. The Arabidopsis (Arabidopsis thaliana) SnRK2 family comprises the abscisic acid (ABA)-activated protein kinases SnRK2.2, SnRK2.3, SnRK2.6, SnRK2.7, and SnRK2.8, and the ABA-independent subclass 1 protein kinases SnRK2.1, SnRK2.4, SnRK2.5, SnRK2.9, and SnRK2.10. ABA-independent SnRK2s act at the posttranscriptional level via phosphorylation of VARICOSE (VCS), a member of the mRNA decapping complex, that catalyzes the first step of 5'mRNA decay. Here, we identified VCS and VARICOSE RELATED (VCR) as interactors and phosphorylation targets of SnRK2.5, SnRK2.6, and SnRK2.10. All three protein kinases phosphorylated Ser-645 and Ser-1156 of VCS, whereas SnRK2.6 and SnRK2.10 also phosphorylated VCS Ser-692 and Ser-680 of VCR. We showed that subclass 1 SnRK2s, VCS, and 5' EXORIBONUCLEASE 4 (XRN4) are involved in regulating root growth under control conditions as well as modulating root system architecture in response to salt stress. Our results suggest interesting patterns of redundancy within subclass 1 SnRK2 protein kinases, with SnRK2.1, SnRK2.5, and SnRK2.9 controlling root growth under nonstress conditions and SnRK2.4 and SnRK2.10 acting mostly in response to salinity. We propose that subclass 1 SnRK2s function in root development under salt stress by affecting the transcript levels of aquaporins, as well as CYP79B2, an enzyme involved in auxin biosynthesis.", "keywords": ["580", "0301 basic medicine", "570", "0303 health sciences", "Arabidopsis Proteins", "Arabidopsis", "Protein Serine-Threonine Kinases", "03 medical and health sciences", "Exoribonucleases", "Life Science", "Salts", "RNA", " Messenger", "Phosphorylation", "Protein Kinases", "Plant Proteins", "Signal Transduction"]}, "links": [{"href": "https://doi.org/10.1104/pp.19.00818"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1104/pp.19.00818", "name": "item", "description": "10.1104/pp.19.00818", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1104/pp.19.00818"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-09-30T00:00:00Z"}}, {"id": "10.1111/ppl.12714", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:51Z", "type": "Journal Article", "created": "2018-03-01", "title": "Anatomical and hormonal description of rootlet primordium development along white lupin cluster root", "description": "<p>Cluster root (CR) is one of the most spectacular plant developmental adaptations to hostile environment. It can be found in a few species from a dozen botanical families, including white lupin (Lupinus albus) in the Fabaceae family. These amazing structures are produced in phosphate\uffe2\uff80\uff90deprived conditions and are made of hundreds of short roots also known as rootlets. White lupin is the only crop bearing CRs and is considered as the model species for CR studies. However, little information is available on CRs atypical development, including the molecular events that trigger their formation. To provide insights on CR formation, we performed an anatomical and cellular description of rootlet development in white lupin. Starting with a classic histological approach, we described rootlet primordium development and defined eight developmental stages from rootlet initiation to their emergence. Due to the major role of hormones in the developmental program of root system, we next focussed on auxin\uffe2\uff80\uff90related mechanisms. We observed the establishment of an auxin maximum through rootlet development in transgenic roots expressing the DR5:GUS auxin reporter. Expression analysis of the main auxin\uffe2\uff80\uff90related genes [TIR, Auxin Response Factor (ARF) and AUX/IAA] during a detailed time course revealed specific expression associated with the formation of the rootlet primordium. We showed that L. albus TRANSPORT INHIBITOR RESPONSE 1b is expressed during rootlet primordium formation and that L. albus AUXIN RESPONSE FACTOR 5 is expressed in the vasculature but absent in the primordium itself. Altogether, our results describe the very early cellular events leading to CR formation and reveal some of the auxin\uffe2\uff80\uff90related mechanisms.</p>", "keywords": ["0301 basic medicine", "racine laterale", "Plant Roots", "inhibiteur de transport d'auxine", "physiologie v\u00e9g\u00e9tale", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "http://aims.fao.org/aos/agrovoc/c_16034", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "Cloning", " Molecular", "Promoter Regions", " Genetic", "Plant Proteins", "580", "http://aims.fao.org/aos/agrovoc/c_25189", "0303 health sciences", "syst\u00e8me racinaire", "Vegetal Biology", "interaction sol racine", "Indoleacetic Acids", "Plants", " Genetically Modified", "http://aims.fao.org/aos/agrovoc/c_27527", "Lupinus", "Lupinus albus", "lupinus albus", "phosphate inorganique", "Biologie v\u00e9g\u00e9tale", "expression des g\u00e8nes", "http://aims.fao.org/aos/agrovoc/c_4464"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/ppl.12714"}, {"href": "https://doi.org/10.1111/ppl.12714"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Physiologia%20Plantarum", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/ppl.12714", "name": "item", "description": "10.1111/ppl.12714", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/ppl.12714"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-26T00:00:00Z"}}, {"id": "10.1126/sciadv.aar3599", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:54Z", "type": "Journal Article", "created": "2018-09-12", "title": "Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Natural nitrogen isotopic signature reveals deprotonation during ammonium transport across living organisms.</p></article>", "keywords": ["0301 basic medicine", "570", "Potassium Channels", "Saccharomyces cerevisiae Proteins", "Nitrogen", "Ammonium deprotonation", "Saccharomyces cerevisiae", "Biochemistry", "630", "03 medical and health sciences", "Ammonia", "Ammonium Compounds", "Cation Transport Proteins", "Research Articles", "Plant Proteins", "AMT-Mep-Rh membrane proteins", "0303 health sciences", "Ion Transport", "Nitrogen Isotopes", "Arabidopsis Proteins", "Sciences bio-m\u00e9dicales et agricoles", "AMT-Mep-Rh proteins", "Hydrogen-Ion Concentration", "3. Good health", "Ammonium transport", "N isotope fractionation", "PH control", "Potassium", "Microorganisms", " Genetically-Modified", "Nitrogen (N)", "Ammonium"]}, "links": [{"href": "https://dipot.ulb.ac.be/dspace/bitstream/2013/277241/4/doi_260868.pdf"}, {"href": "https://doi.org/10.1126/sciadv.aar3599"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1126/sciadv.aar3599", "name": "item", "description": "10.1126/sciadv.aar3599", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1126/sciadv.aar3599"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-07T00:00:00Z"}}, {"id": "10.1126/science.ade9204", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:55Z", "type": "Journal Article", "created": "2023-01-19", "title": "Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis", "description": "<p>             Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the             Lotus japonicus             Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.           </p", "keywords": ["Lipopolysaccharides", "0301 basic medicine", "0303 health sciences", "Cell Membrane/metabolism", "Cell Membrane", "Single-Domain Antibodies", "Plant Proteins/genetics", "Symbiosis/physiology", "Root Nodules", " Plant/metabolism", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Medicago truncatula", "Lotus", "Root Nodules", " Plant", "Symbiosis", "Lipopolysaccharides/metabolism", "Plant Proteins", "Signal Transduction"]}, "links": [{"href": "https://www.science.org/doi/pdf/10.1126/science.ade9204"}, {"href": "https://doi.org/10.1126/science.ade9204"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1126/science.ade9204", "name": "item", "description": "10.1126/science.ade9204", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1126/science.ade9204"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-20T00:00:00Z"}}, {"id": "10.1146/annurev-arplant-070623-105324", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:01Z", "type": "Journal Article", "created": "2024-01-26", "title": "Metal Homeostasis in Land Plants: A Perpetual Balancing Act Beyond the Fulfilment of Metalloproteome Cofactor Demands", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>One of life's decisive innovations was to harness the catalytic power of metals for cellular chemistry. With life's expansion, global atmospheric and biogeochemical cycles underwent dramatic changes. Although initially harmful, they permitted the evolution of multicellularity and the colonization of land. In land plants as primary producers, metal homeostasis faces heightened demands, in part because soil is a challenging environment for nutrient balancing. To avoid both nutrient metal limitation and metal toxicity, plants must maintain the homeostasis of metals within tighter limits than the homeostasis of other minerals. This review describes the present model of protein metalation and sketches its transfer from unicellular organisms to land plants as complex multicellular organisms. The inseparable connection between metal and redox homeostasis increasingly draws our attention to more general regulatory roles of metals. Mineral co-option, the use of nutrient or other metals for functions other than nutrition, is an emerging concept beyond that of nutritional immunity.</p></article>", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Metals", "13. Climate action", "Metalloproteins", "Homeostasis", "Embryophyta", "Plant Proteins"], "contacts": [{"organization": "Kr\u00e4mer, Ute", "roles": ["creator"]}]}, "links": [{"href": "https://www.annualreviews.org/content/journals/10.1146/annurev-arplant-070623-105324?crawler=true&mimetype=application/pdf"}, {"href": "https://doi.org/10.1146/annurev-arplant-070623-105324"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Annual%20Review%20of%20Plant%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1146/annurev-arplant-070623-105324", "name": "item", "description": "10.1146/annurev-arplant-070623-105324", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1146/annurev-arplant-070623-105324"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-07-22T00:00:00Z"}}, {"id": "10.3390/genes11091011", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:21:43Z", "type": "Journal Article", "created": "2020-08-27", "title": "Phenotyping in Arabidopsis and Crops\u2014Are We Addressing the Same Traits? A Case Study in Tomato", "description": "<p>The convenient model Arabidopsis thaliana has allowed tremendous advances in plant genetics and physiology, in spite of only being a weed. It has also unveiled the main molecular networks governing, among others, abiotic stress responses. Through the use of the latest genomic tools, Arabidopsis research is nowadays being translated to agronomically interesting crop models such as tomato, but at a lagging pace. Knowledge transfer has been hindered by invariable differences in plant architecture and behaviour, as well as the divergent direct objectives of research in Arabidopsis vs. crops compromise transferability. In this sense, phenotype translation is still a very complex matter. Here, we point out the challenges of \uffe2\uff80\uff9ctranslational phenotyping\uffe2\uff80\uff9d in the case study of drought stress phenotyping in Arabidopsis and tomato. After briefly defining and describing drought stress and survival strategies, we compare drought stress protocols and phenotyping techniques most commonly used in the two species, and discuss their potential to gain insights, which are truly transferable between species. This review is intended to be a starting point for discussion about translational phenotyping approaches among plant scientists, and provides a useful compendium of methods and techniques used in modern phenotyping for this specific plant pair as a case study.</p>", "keywords": ["Crops", " Agricultural", "0301 basic medicine", "2. Zero hunger", "9. Industry and infrastructure", "Arabidopsis", "Review", "15. Life on land", "6. Clean water", "Droughts", "03 medical and health sciences", "Phenotype", "Solanum lycopersicum", "Gene Expression Regulation", " Plant", "Stress", " Physiological", "Arabidopsis; tomato; phenotyping; drought stress; translational phenotyping; osmotic stress; Dehydration; Arabidopsis thaliana; Solanum lycopersicum; Lycopersicon esculentum", "Plant Proteins"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/898415/2/genes-11-01011-v3.pdf"}, {"href": "https://iris.unito.it/bitstream/2318/1757296/1/genes-11-01011-v3.pdf"}, {"href": "https://www.mdpi.com/2073-4425/11/9/1011/pdf"}, {"href": "https://doi.org/10.3390/genes11091011"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes11091011", "name": "item", "description": "10.3390/genes11091011", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes11091011"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-27T00:00:00Z"}}, {"id": "10.1371%2fjournal.pone.0044697", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:12Z", "type": "Journal Article", "created": "2012-09-11", "title": "A Novel Stress-Induced Sugarcane Gene Confers Tolerance To Drought, Salt And Oxidative Stress In Transgenic Tobacco Plants", "description": "Drought is a major abiotic stress that affects crop productivity worldwide. Sugarcane can withstand periods of water scarcity during the final stage of culm maturation, during which sucrose accumulation occurs. Meanwhile, prolonged periods of drought can cause severe plant losses.In a previous study, we evaluated the transcriptome of drought-stressed plants to better understand sugarcane responses to drought. Among the up-regulated genes was Scdr1 (sugarcane drought-responsive 1). The aim of the research reported here was to characterize this gene. Scdr1 encodes a putative protein containing 248 amino acids with a large number of proline (19%) and cysteine (13%) residues. Phylogenetic analysis showed that ScDR1is in a clade with homologs from other monocotyledonous plants, separate from those of dicotyledonous plants. The expression of Scdr1 in different varieties of sugarcane plants has not shown a clear association with drought tolerance.The overexpression of Scdr1 in transgenic tobacco plants increased their tolerance to drought, salinity and oxidative stress, as demonstrated by increased photosynthesis, water content, biomass, germination rate, chlorophyll content and reduced accumulation of ROS. Physiological parameters, such as transpiration rate (E), net photosynthesis (A), stomatal conductance (gs) and internal leaf CO(2) concentration, were less affected by abiotic stresses in transgenic Scdr1 plants compared with wild-type plants. Overall, our results indicated that Scdr1 conferred tolerance to multiple abiotic stresses, highlighting the potential of this gene for biotechnological applications.", "keywords": ["Chlorophyll", "Nicotiana", "0301 basic medicine", "Sucrose", "Science", "Molecular Sequence Data", "03 medical and health sciences", "Amino Acid Sequence", "Biomass", "Transgenes", "Photosynthesis", "Phylogeny", "Plant Proteins", "2. Zero hunger", "0303 health sciences", "Base Sequence", "Sequence Homology", " Amino Acid", "Q", "R", "Water", "15. Life on land", "Plants", " Genetically Modified", "6. Clean water", "Droughts", "Saccharum", "Oxidative Stress", "Medicine", "Salts", "Reactive Oxygen Species", "Research Article", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1371%2fjournal.pone.0044697"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371%2fjournal.pone.0044697", "name": "item", "description": "10.1371%2fjournal.pone.0044697", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371%2fjournal.pone.0044697"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-09-11T00:00:00Z"}}, {"id": "10.1371/journal.pbio.3002127", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:13Z", "type": "Journal Article", "created": "2023-05-18", "title": "A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus", "description": "<p>Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception.</p", "keywords": ["570", "QH301-705.5", "ANZSRC::30 Agricultural", "MESORHIZOBIUM-LOTI", "Plant Roots", "veterinary and food sciences", "ROOT", "Polysaccharides", "Gene Expression Regulation", " Plant", "Mycorrhizae", "CELL", "Biology (General)", "Symbiosis", "Glucans", "Plant Proteins", "FUNGAL", "PERCEPTION", "ANZSRC::31 Biological sciences", "LAMINARIN", "Phosphotransferases", "500", "TRANSFORMATION", "EVOLUTION", "ANZSRC::32 Biomedical and clinical sciences", "DEFENSE RESPONSES", "Mutation", "Lotus", "EXOPOLYSACCHARIDE", "Root Nodules", " Plant", "Research Article", "Rhizobium"]}, "links": [{"href": "https://doi.org/10.1371/journal.pbio.3002127"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pbio.3002127", "name": "item", "description": "10.1371/journal.pbio.3002127", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pbio.3002127"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-05-18T00:00:00Z"}}, {"id": "10.3390/su12051962", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:54Z", "type": "Journal Article", "created": "2020-03-04", "title": "Transitioning European Protein-Rich Food Consumption and Production towards More Sustainable Patterns\u2014Strategies and Policy Suggestions", "description": "<p>Global and European diets have shifted towards greater consumption of animal proteins. Recent studies urge reversals of these trends and call for a rapid transition towards adoption of more plant-based diets. This paper explored mechanisms to increase the production and consumption of plant-proteins in Europe by 2030, using participatory backcasting. We identified pathways to the future (strategies), as well as interim milestones, barriers, opportunities and actions, with key European stakeholders in the agri-food chain. Results show that four strategies could be implemented to achieve the desired future: increased research and development, enriched consumer education and awareness, improved and connected supply and value chains and public policy supports. Actions needed to reach milestones were required immediately, reinforcing the need for urgent actions to tackle the protein challenge. This study concretely detailed how idealized dietary futures can be achieved in a real-world context. It can support EU protein transition by informing policy makers and the broader public on potential ways to move towards a more sustainable plant-based future. The outputs of this analysis have the potential to be combined with dietary scenarios to develop more temporally explicit models of future dietary changes and how to reach them.</p>", "keywords": ["future", "2. Zero hunger", "Agricultura", "pathways", "food security", "01 natural sciences", "stakeholders", "Econom\u00eda", "12. Responsible consumption", "sustainable agriculture", "meat substitutes", "Medio Ambiente", "plant protein", "13. Climate action", "backcasting", "value chain", "meat substitution", "europe", "diet", "plant proteins", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://www.mdpi.com/2071-1050/12/5/1962/pdf"}, {"href": "https://www.mdpi.com/2071-1050/12/5/1962/pdf"}, {"href": "https://doi.org/10.3390/su12051962"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/su12051962", "name": "item", "description": "10.3390/su12051962", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/su12051962"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-04T00:00:00Z"}}, {"id": "10182/16842", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:25:36Z", "type": "Journal Article", "created": "2023-05-18", "title": "A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus", "description": "<p>Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception.</p", "keywords": ["570", "QH301-705.5", "ANZSRC::30 Agricultural", "MESORHIZOBIUM-LOTI", "Plant Roots", "veterinary and food sciences", "ROOT", "Polysaccharides", "Gene Expression Regulation", " Plant", "Mycorrhizae", "CELL", "Biology (General)", "Symbiosis", "Glucans", "Plant Proteins", "FUNGAL", "PERCEPTION", "ANZSRC::31 Biological sciences", "LAMINARIN", "Phosphotransferases", "500", "TRANSFORMATION", "EVOLUTION", "ANZSRC::32 Biomedical and clinical sciences", "DEFENSE RESPONSES", "Mutation", "Lotus", "EXOPOLYSACCHARIDE", "Root Nodules", " Plant", "Research Article", "Rhizobium"]}, "links": [{"href": "https://doi.org/10182/16842"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10182/16842", "name": "item", "description": "10182/16842", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10182/16842"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-05-18T00:00:00Z"}}, {"id": "10138/356895", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:25:35Z", "type": "Journal Article", "created": "2023-03-08", "title": "The giant diploid faba genome unlocks variation in a global protein crop", "description": "Abstract                   <p>                     Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity                     1                     . However, plant protein production is hampered by the lack of a cool-season legume equivalent to soybean in agronomic value                     2                     . Faba bean (                     Vicia faba                     L.) has a high yield potential and is well suited for cultivation in temperate regions, but genomic resources are scarce. Here, we report a high-quality chromosome-scale assembly of the faba bean genome and show that it has expanded to a massive 13\uffe2\uff80\uff89Gb in size through an imbalance between the rates of amplification and elimination of retrotransposons and satellite repeats. Genes and recombination events are evenly dispersed across chromosomes and the gene space is remarkably compact considering the genome size, although with substantial copy number variation driven by tandem duplication. Demonstrating practical application of the genome sequence, we develop a targeted genotyping assay and use high-resolution genome-wide association analysis to dissect the genetic basis of seed size and hilum colour. The resources presented constitute a genomics-based breeding platform for faba bean, enabling breeders and geneticists to accelerate the\uffc2\uffa0improvement of sustainable protein production across the\uffc2\uffa0Mediterranean, subtropical and northern temperate agroecological zones.                   </p", "keywords": ["Crops", " Agricultural", "DNA Copy Number Variations", "Retroelements", "[SDV]Life Sciences [q-bio]", "DNA", " Satellite", "Genes", " Plant", "630", "Article", "Chromosomes", " Plant", "Plant Proteins", "Recombination", " Genetic", "2. Zero hunger", "Geography", "Gene Amplification", "Genetic Variation", "Genomics", "15. Life on land", "11831 Plant biology", "Diploidy", "Agronomy", "metabolism ; Genome-Wide Association Study ; Plant Proteins ; genetics ; Plant Breeding ; Vicia faba ; DNA Copy Number Variations ; Diploidy", "Vicia faba", "[SDV] Life Sciences [q-bio]", "Plant Breeding", "Genetics", " developmental biology", " physiology", "13. Climate action", "Seeds", "Genome", " Plant", "info:eu-repo/classification/ddc/500", "Genome-Wide Association Study"]}, "links": [{"href": "https://doi.org/10138/356895"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10138/356895", "name": "item", "description": "10138/356895", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10138/356895"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-26T00:00:00Z"}}, {"id": "10261/369001", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:25:43Z", "type": "Journal Article", "created": "2024-06-26", "title": "Zinc mediates control of nitrogen fixation via transcription factor filamentation", "description": "Abstract<p>Plants adapt to fluctuating environmental conditions by adjusting their metabolism and gene expression to maintain fitness1. In legumes, nitrogen homeostasis is maintained by balancing nitrogen acquired from soil resources with nitrogen fixation by symbiotic bacteria in root nodules2\uffe2\uff80\uff938. Here we show that zinc, an essential plant micronutrient, acts as an intracellular second messenger that connects environmental changes to transcription factor control of metabolic activity in root nodules. We identify a transcriptional regulator, FIXATION UNDER NITRATE (FUN), which acts as a sensor, with zinc controlling the transition between an inactive filamentous megastructure and an active transcriptional regulator. Lower zinc concentrations in the nodule, which we show occur in response to higher levels of soil nitrate, dissociates the filament and activates FUN. FUN then directly targets multiple pathways to initiate breakdown of the nodule. The zinc-dependent filamentation mechanism thus establishes a concentration readout to adapt nodule function to the environmental nitrogen conditions. In a wider perspective, these results have implications for understanding the roles of metal ions in integration of environmental signals with plant development and optimizing delivery of fixed nitrogen in legume crops.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Nitrates", "Nitrogen", "Second Messenger Systems", "Article", "Zinc", "03 medical and health sciences", "Plant signalling", "Gene Expression Regulation", " Plant", "Nitrogen Fixation", "Lotus", "Root Nodules", " Plant", "Symbiosis", "Rhizobial symbiosis", "Plant Proteins", "Transcription Factors"]}, "links": [{"href": "https://doi.org/10261/369001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/369001", "name": "item", "description": "10261/369001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/369001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-06-26T00:00:00Z"}}, {"id": "10261/393341", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:25:44Z", "type": "Journal Article", "created": "2025-01-15", "title": "Autoactive CNGC15 enhances root endosymbiosis in legume and wheat", "description": "Abstract           <p>Nutrient acquisition is crucial for sustaining life. Plants develop beneficial intracellular partnerships with arbuscular mycorrhiza (AM) and nitrogen-fixing bacteria to surmount the scarcity of soil nutrients and tap into atmospheric dinitrogen, respectively1,2. Initiation of these root endosymbioses requires symbiont-induced oscillations in nuclear calcium (Ca2+) concentrations in root cells3. How the nuclear-localized ion channels, cyclic nucleotide-gated channel (CNGC) 15 and DOESN\uffe2\uff80\uff99T MAKE INFECTIONS1 (DMI1)4 are coordinated to specify symbiotic-induced nuclear Ca2+ oscillations remains unknown. Here we discovered an autoactive CNGC15 mutant that generates spontaneous low-frequency Ca2+ oscillations. While CNGC15 produces nuclear Ca2+ oscillations via a gating mechanism involving its helix 1, DMI1 acts as a pacemaker to specify the frequency of the oscillations. We demonstrate that the specificity of symbiotic-induced nuclear Ca2+ oscillations is encoded in its frequency. A high frequency activates endosymbiosis programmes, whereas a low frequency modulates phenylpropanoid pathways. Consequently, the autoactive cngc15 mutant, which is capable of generating both frequencies, has increased flavonoids that enhance AM, root nodule symbiosis and nutrient acquisition. We transferred this trait to wheat, resulting in field-grown wheat with increased AM colonization and nutrient acquisition. Our findings reveal a new strategy to boost endosymbiosis in the field and reduce inorganic fertilizer use while sustaining plant growth.</p", "keywords": ["Cell Nucleus", "Calcium signalling", "Cyclic Nucleotide-Gated Cation Channels", "Fabaceae", "Plant Roots", "Plant breeding", "Article", "Mycorrhizae", "Mutation", "Medicago truncatula", "Calcium", "Calcium Signaling", "Arbuscular mycorrhiza", "Symbiosis", "Triticum", "Rhizobial symbiosis", "Plant Proteins"]}, "links": [{"href": "https://doi.org/10261/393341"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/393341", "name": "item", "description": "10261/393341", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/393341"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-15T00:00:00Z"}}, {"id": "10261/395709", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:25:44Z", "type": "Journal Article", "created": "2022-10-24", "title": "Regulation and Function of a Polarly Localized Lignin Barrier in the Exodermis", "description": "ABSTRACT<p>Multicellular organisms control interactions with their environment through the development of specialized barriers in specific cell types. A conserved barrier in plant roots is the endodermal Casparian strip (CS). The CS is made of polymerized lignin and forms a ring-like structure that seals the apoplastic space between the endodermal cells. Most angiosperms also have another root cell type, the exodermis, that is reported to form a barrier. Our understanding of exodermal developmental and molecular regulation, as well as function, is limited as this cell type is absent from the model speciesArabidopsis thaliana. Using tomato (Solanum lycopersicum) as a model system we demonstrate that in this species, the exodermis does not form a CS. Instead, it forms a polar lignin cap with an equivalent barrier function to the endodermal CS. We demonstrate that although endodermal regulators are conserved between Arabidopsis and tomato, exodermal differentiation occurs by a distinct regulatory pathway involving theSlSCZandSlEXO1transcription factors. Although the exodermis and endodermis both produce barriers that restrict mineral ion uptake, they have unique and overlapping roles in their selectivity. Whether conservation and similarities between the endodermis and exodermis exist in other species remains to be determined. Nonetheless, in tomato, these distinct lignin structures have a convergent function with different genetic regulations.</p", "keywords": ["580", "0301 basic medicine", "570", "0303 health sciences", "Plant", "Lignin", "Plant Roots", "Article", "03 medical and health sciences", "Solanum lycopersicum", "Gene Expression Regulation", "Lignin/metabolism; Solanum lycopersicum/genetics; Solanum lycopersicum/metabolism; Solanum lycopersicum/growth & development; Solanum lycopersicum/physiology; Gene Expression Regulation", " Plant; Plant Roots/metabolism; Plant Roots/growth & development; Plant Roots/genetics; Plant Proteins/metabolism; Plant Proteins/genetics; Transcription Factors/metabolism; Transcription Factors/genetics", "Plant Proteins", "Transcription Factors"]}, "links": [{"href": "https://escholarship.org/content/qt52920703/qt52920703.pdf"}, {"href": "https://doi.org/10261/395709"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Plants", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/395709", "name": "item", "description": "10261/395709", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/395709"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-10-21T00:00:00Z"}}, {"id": "11104/0341036", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:25:56Z", "type": "Journal Article", "created": "2022-07-18", "title": "Ethylene inhibits rice root elongation in compacted soil via ABA- and auxin-mediated mechanisms", "description": "<p>             Soil compaction represents a major agronomic challenge, inhibiting root elongation and impacting crop yields. Roots use ethylene to sense soil compaction as the restricted air space causes this gaseous signal to accumulate around root tips. Ethylene inhibits root elongation and promotes radial expansion in compacted soil, but its mechanistic basis remains unclear. Here, we report that ethylene promotes abscisic acid (ABA) biosynthesis and cortical cell radial expansion. Rice mutants of ABA biosynthetic genes had attenuated cortical cell radial expansion in compacted soil, leading to better penetration. Soil compaction-induced ethylene also up-regulates the auxin biosynthesis gene             OsYUC8             . Mutants lacking OsYUC8 are better able to penetrate compacted soil. The auxin influx transporter OsAUX1 is also required to mobilize auxin from the root tip to the elongation zone during a root compaction response. Moreover,             osaux1             mutants penetrate compacted soil better than the wild-type roots and do not exhibit cortical cell radial expansion. We conclude that ethylene uses auxin and ABA as downstream signals to modify rice root cell elongation and radial expansion, causing root tips to swell and reducing their ability to penetrate compacted soil.           </p", "keywords": ["roots", "0301 basic medicine", "570", "Cell biology", "Arabidopsis", "Biophysics", "Plant Science", "Plant Roots", "Biochemistry", "Gene", "Catalysis", "Mixed Function Oxygenases", "Molecular Mechanisms of Plant Development and Regulation", "soil compaction", "Agricultural and Biological Sciences", "Soil", "Abscisic acid", "Ethylene", "03 medical and health sciences", "aba", "ethylene", "Auxin", "Elongation", "Biology", "Plant Proteins", "580", "2. Zero hunger", "0303 health sciences", "Multidisciplinary", "Indoleacetic Acids", "Mutant", "Life Sciences", "Oryza", "Plant Nutrient Uptake and Signaling Pathways", "Biological Sciences", "Ethylenes", "15. Life on land", "Materials science", "Root Aeration", "Chemistry", "ABA", "Plant Responses to Flooding Stress", "Ultimate tensile strength", "Mutation", "Metallurgy", "auxin", "Abscisic Acid"]}, "links": [{"href": "https://pnas.org/doi/pdf/10.1073/pnas.2201072119"}, {"href": "https://doi.org/11104/0341036"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11104/0341036", "name": "item", "description": "11104/0341036", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11104/0341036"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-18T00:00:00Z"}}, {"id": "11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:25:57Z", "type": "Journal Article", "created": "2024-05-01", "title": "Arabinosylation of cell wall extensin is required for the directional response to salinity in roots", "description": "Abstract                   <p>Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity.</p", "keywords": ["580", "0301 basic medicine", "2. Zero hunger", "570", "Salinity", "0303 health sciences", "Glycosylation", "Arabidopsis Proteins", "Arabidopsis", "Breakthrough Report", "Sodium Chloride", "15. Life on land", "Arabinose", "Plant Roots", "Gravitropism", "03 medical and health sciences", "Cell Wall", "Gene Expression Regulation", " Plant", "Life Science", "Glycoproteins", "Plant Proteins"]}, "links": [{"href": "https://academic.oup.com/plcell/article-pdf/36/9/3328/59006321/koae135.pdf"}, {"href": "https://doi.org/11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Plant%20Cell", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2", "name": "item", "description": "11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-01T00:00:00Z"}}, {"id": "11381/2905288", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:26:00Z", "type": "Journal Article", "created": "2021-11-29", "title": "Immunomodulatory peptides\u2014A promising source for novel functional food production and drug discovery", "description": "Immunomodulatory peptides are a complex class of bioactive peptides that encompasses substances with different mechanisms of action. Immunomodulatory peptides could also be used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics. Thus, immunomodulatory peptides in food of plant origin could be regarded both as valuable suplements of novel functional food preparation and/or as precursors or possible active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system. Given variety of mechanisms, different tests are required to assess effects of immunomodulatory peptides. Some of those effects show good correlation with in vivo results but others, less so. Certain plant peptides, such as defensins, show both immunomodulatory and antimicrobial effect, which makes them interesting candidates for preparation of functional food and feed, as well as templates for design of synthetic peptides.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Vaccines", "570", "0303 health sciences", "Functional food", "Cryptic peptide", "610", "Mechanism of action", "3. Good health", "Defensins", "Immunomodulation", "03 medical and health sciences", "Immunomodulatory peptide", "Defensin", "Functional Food", "Drug Discovery", "Humans", "Peptides", "Settore BIOS-10/A - Biologia cellulare e applicata", "Plant Proteins"]}, "links": [{"href": "https://doi.org/11381/2905288"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Peptides", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11381/2905288", "name": "item", "description": "11381/2905288", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/2905288"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "11577/3392826", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:26:02Z", "type": "Journal Article", "created": "2021-05-18", "title": "Innovation, conservation, and repurposing of gene function in root cell type development", "description": "Plant species have evolved myriads of solutions, including complex cell type development and regulation, to adapt to dynamic environments. To understand this cellular diversity, we profiled tomato root cell type translatomes. Using xylem differentiation in tomato, examples of functional innovation, repurposing, and conservation of transcription factors are described, relative to the model plant Arabidopsis. Repurposing and innovation of genes are further observed within an exodermis regulatory network and illustrate its function. Comparative translatome analyses of rice, tomato, and Arabidopsis cell populations suggest increased expression conservation of root meristems compared with other homologous populations. In addition, the functions of constitutively expressed genes are more conserved than those of cell type/tissue-enriched genes. These observations suggest that higher order properties of cell type and pan-cell type regulation are evolutionarily conserved between plants and animals.", "keywords": ["root development", "translatomes", "General Biochemistry", "Genetics and Molecular Biology", "Green Fluorescent Proteins", "Meristem", "Arabidopsis", "cell types; evolution; exodermis; gene regulation; rice; root development; tomato; translatomes; xylem", "tomato", "xylem", "Genes", " Plant", "Plant Roots", "Inventions", "Solanum lycopersicum", "Species Specificity", "Gene Expression Regulation", " Plant", "Xylem", "evolution", "Gene Regulatory Networks", "Promoter Regions", " Genetic", "Plant Proteins", "2. Zero hunger", "exodermis", "rice", "15. Life on land", "Protein Biosynthesis", "cell types", "gene regulation", "Transcription Factors"]}, "links": [{"href": "https://www.research.unipd.it/bitstream/11577/3392826/2/PIIS0092867421005043.pdf"}, {"href": "https://doi.org/11577/3392826"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Cell", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11577/3392826", "name": "item", "description": "11577/3392826", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11577/3392826"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-01T00:00:00Z"}}, {"id": "1854/LU-8632050", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:26:14Z", "type": "Journal Article", "created": "2019-09-30", "title": "SnRK2 Protein Kinases and mRNA Decapping Machinery Control Root Development and Response to Salt", "description": "SNF1-RELATED PROTEIN KINASES 2 (SnRK2) are important components of early osmotic and salt stress signaling pathways in plants. The Arabidopsis (Arabidopsis thaliana) SnRK2 family comprises the abscisic acid (ABA)-activated protein kinases SnRK2.2, SnRK2.3, SnRK2.6, SnRK2.7, and SnRK2.8, and the ABA-independent subclass 1 protein kinases SnRK2.1, SnRK2.4, SnRK2.5, SnRK2.9, and SnRK2.10. ABA-independent SnRK2s act at the posttranscriptional level via phosphorylation of VARICOSE (VCS), a member of the mRNA decapping complex, that catalyzes the first step of 5'mRNA decay. Here, we identified VCS and VARICOSE RELATED (VCR) as interactors and phosphorylation targets of SnRK2.5, SnRK2.6, and SnRK2.10. All three protein kinases phosphorylated Ser-645 and Ser-1156 of VCS, whereas SnRK2.6 and SnRK2.10 also phosphorylated VCS Ser-692 and Ser-680 of VCR. We showed that subclass 1 SnRK2s, VCS, and 5' EXORIBONUCLEASE 4 (XRN4) are involved in regulating root growth under control conditions as well as modulating root system architecture in response to salt stress. Our results suggest interesting patterns of redundancy within subclass 1 SnRK2 protein kinases, with SnRK2.1, SnRK2.5, and SnRK2.9 controlling root growth under nonstress conditions and SnRK2.4 and SnRK2.10 acting mostly in response to salinity. We propose that subclass 1 SnRK2s function in root development under salt stress by affecting the transcript levels of aquaporins, as well as CYP79B2, an enzyme involved in auxin biosynthesis.", "keywords": ["0301 basic medicine", "570", "Arabidopsis", "Protein Serine-Threonine Kinases", "03 medical and health sciences", "HYPEROSMOTIC STRESS", "Life Science", "RNA", " Messenger", "TRITICUM-AESTIVUM L.", "Phosphorylation", "DIFFERENT PHOSPHORYLATION MECHANISMS", "Plant Proteins", "580", "0303 health sciences", "IDENTIFICATION", "Arabidopsis Proteins", "Biology and Life Sciences", "ABSCISIC-ACID", "ARABIDOPSIS", "GENE", "FAMILY", "OSMOTIC STRESSES", "Exoribonucleases", "Salts", "DECAY", "Protein Kinases", "Signal Transduction"]}, "links": [{"href": "https://doi.org/1854/LU-8632050"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1854/LU-8632050", "name": "item", "description": "1854/LU-8632050", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1854/LU-8632050"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-09-30T00:00:00Z"}}, {"id": "2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/277241", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:26:34Z", "type": "Journal Article", "created": "2018-09-12", "title": "Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Natural nitrogen isotopic signature reveals deprotonation during ammonium transport across living organisms.</p></article>", "keywords": ["0301 basic medicine", "570", "Potassium Channels", "Saccharomyces cerevisiae Proteins", "Nitrogen", "Ammonium deprotonation", "Saccharomyces cerevisiae", "Biochemistry", "630", "03 medical and health sciences", "Ammonia", "Ammonium Compounds", "Cation Transport Proteins", "Research Articles", "Plant Proteins", "AMT-Mep-Rh membrane proteins", "0303 health sciences", "Ion Transport", "Nitrogen Isotopes", "Arabidopsis Proteins", "Sciences bio-m\u00e9dicales et agricoles", "AMT-Mep-Rh proteins", "Hydrogen-Ion Concentration", "3. Good health", "Ammonium transport", "N isotope fractionation", "PH control", "Potassium", "Microorganisms", " Genetically-Modified", "Nitrogen (N)", "Ammonium"]}, "links": [{"href": "https://repositorio.ulisboa.pt/bitstream/10451/60845/1/sciadv.aar3599.pdf"}, {"href": "https://dipot.ulb.ac.be/dspace/bitstream/2013/277241/4/doi_260868.pdf"}, {"href": "https://doi.org/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/277241"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/277241", "name": "item", "description": "2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/277241", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/277241"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-07T00:00:00Z"}}, {"id": "2980991993", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:27:03Z", "type": "Journal Article", "created": "2019-10-16", "title": "Phosphorus-acquisition strategies of canola, wheat and barley in soil amended with sewage sludges", "description": "Abstract<p>Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition strategies of three main crops are affected by the application of sewage sludges, compared with a mineral P fertilizer. We carried out a 3-months greenhouse pot experiment and compared the response of P-acquisition traits among wheat, barley and canola in a soil amended with three sludges or a mineral P fertilizer. Results showed that the P-acquisition strategy differed among crops. Compared with canola, wheat and barley had a higher specific root length and a greater root carboxylate release and they acquired as much P from sludge as from mineral P. By contrast, canola shoot P content was greater with sludge than with mineral P. This was attributed to a higher root-released acid phosphatase activity which promoted the mineralization of sludge-derived P-organic. This study showed that contrasted P-acquisition strategies of crops allows increased use of renewable P resources by optimizing combinations of crop and the type of P fertilizer applied within the cropping system.</p", "keywords": ["Calcium Phosphates", "Crops", " Agricultural", "0106 biological sciences", "phosphatase activity", "N\u00e4hrstoffaufnahme", "carboxylate", "Phytic Acid", "Acid Phosphatase", "[SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy", "Carboxylic Acids", "organic P fertilizer", " mineral P fertilizer", " carboxylate", " phosphatase activity", "Plant Roots", "01 natural sciences", "630", "Article", "12. Responsible consumption", "Soil", "Boden", "Species Specificity", "ddc:630", "Humans", "Fertilizers", "Triticum", "Plant Proteins", "2. Zero hunger", "Plant Stems", "Sewage", "Brassica rapa", "Agriculture", "Biological Transport", "Hordeum", "Phosphorus", "Phosphor", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "0401 agriculture", " forestry", " and fisheries", "G\u00e4rrest", "mineral P fertilizer", "organic P fertilizer"]}, "links": [{"href": "https://www.nature.com/articles/s41598-019-51204-x.pdf"}, {"href": "https://doi.org/2980991993"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2980991993", "name": "item", "description": "2980991993", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2980991993"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-16T00:00:00Z"}}, {"id": "3147315065", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:27:20Z", "type": "Journal Article", "created": "2020-05-31", "title": "Distinct signalling routes mediates intercellular and intracellular rhizobial infection in Lotus japonicus", "description": "Abstract<p>Rhizobial infection of legume roots during development of nitrogen fixing root nodules occurs either intracellularly though plant derived infection threads traversing the epidermal and cortical cell layers to deliver the bacteria or intercellularly via bacterial entry between epidermal plant cells. Although, around 25% of all legume genera are postulated to be intercellularly infected, the pathways and mechanisms supporting this process has remained virtually unexplored due to lack of genetically amenable legumes that have this infection mode. In this study, we report that the model legume Lotus japonicus is infected intercellularly by Rhizobium sp. IRBG74 and demonstrate that the resources available in Lotus enable insight into the genetic requirements and the fine-tuning of the pathway governing intercellular infection. Inoculation of Lotus mutants shows that Ern1 and RinRK1 are dispensable for intercellular infection in contrast to intracellular infection. Other symbiotic genes, including Nfr5, SymRK, CCaMK, Epr3, Cyclops, Nin, Nsp1, Nsp2, Cbs and Vpy1 are equally important for both entry modes. Comparative RNAseq analysis of roots inoculated with IRBG74 revealed a distinctive transcriptome response compared to intracellular colonization. In particular, a number of cytokinin-related genes were differentially regulated. Corroborating this observation cyp735A and ipt4 cytokinin biosynthesis mutants were significantly affected in their nodulation with IRBG74 while lhk1 cytokinin receptor mutants did not form any nodules. These results indicate that a differential requirement for cytokinin signalling conditions intercellular rhizobial entry and highlight the distinct modalities of the inter- and intra-cellular infection mechanisms.</p", "keywords": ["name=Physiology", "580", "0301 basic medicine", "/dk/atira/pure/subjectarea/asjc/1300/1314", "0303 health sciences", "/dk/atira/pure/subjectarea/asjc/1300/1311", "571", "Regular Issue", "name=Genetics", "/dk/atira/pure/subjectarea/asjc/1100/1110", "Plant Roots", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Calcium-Calmodulin-Dependent Protein Kinases", "Lotus", "name=Plant Science", "Root Nodules", " Plant", "Plant Proteins", "Rhizobium", "Signal Transduction"]}, "links": [{"href": "http://academic.oup.com/plphys/article-pdf/185/3/1131/37166752/kiaa049.pdf"}, {"href": "https://doi.org/3147315065"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3147315065", "name": "item", "description": "3147315065", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3147315065"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-31T00:00:00Z"}}, {"id": "36656954", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:27:35Z", "type": "Journal Article", "created": "2023-01-19", "title": "Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis", "description": "<p>             Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the             Lotus japonicus             Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.           </p", "keywords": ["Lipopolysaccharides", "0301 basic medicine", "0303 health sciences", "Cell Membrane/metabolism", "Cell Membrane", "Single-Domain Antibodies", "Plant Proteins/genetics", "Symbiosis/physiology", "Root Nodules", " Plant/metabolism", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Medicago truncatula", "Lotus", "Root Nodules", " Plant", "Symbiosis", "Lipopolysaccharides/metabolism", "Plant Proteins", "Signal Transduction"]}, "links": [{"href": "https://www.science.org/doi/pdf/10.1126/science.ade9204"}, {"href": "https://doi.org/36656954"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "36656954", "name": "item", "description": "36656954", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/36656954"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-20T00:00:00Z"}}, {"id": "38277698", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:27:38Z", "type": "Journal Article", "created": "2024-01-26", "title": "Metal Homeostasis in Land Plants: A Perpetual Balancing Act Beyond the Fulfilment of Metalloproteome Cofactor Demands", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>One of life's decisive innovations was to harness the catalytic power of metals for cellular chemistry. With life's expansion, global atmospheric and biogeochemical cycles underwent dramatic changes. Although initially harmful, they permitted the evolution of multicellularity and the colonization of land. In land plants as primary producers, metal homeostasis faces heightened demands, in part because soil is a challenging environment for nutrient balancing. To avoid both nutrient metal limitation and metal toxicity, plants must maintain the homeostasis of metals within tighter limits than the homeostasis of other minerals. This review describes the present model of protein metalation and sketches its transfer from unicellular organisms to land plants as complex multicellular organisms. The inseparable connection between metal and redox homeostasis increasingly draws our attention to more general regulatory roles of metals. Mineral co-option, the use of nutrient or other metals for functions other than nutrition, is an emerging concept beyond that of nutritional immunity.</p></article>", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Metals", "13. Climate action", "Metalloproteins", "Homeostasis", "Embryophyta", "Plant Proteins"], "contacts": [{"organization": "Kr\u00e4mer, Ute", "roles": ["creator"]}]}, "links": [{"href": "https://www.annualreviews.org/content/journals/10.1146/annurev-arplant-070623-105324?crawler=true&mimetype=application/pdf"}, {"href": "https://doi.org/38277698"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Annual%20Review%20of%20Plant%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "38277698", "name": "item", "description": "38277698", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/38277698"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-07-22T00:00:00Z"}}, {"id": "PMC10630511", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:29:41Z", "type": "Journal Article", "created": "2023-11-07", "title": "Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus", "description": "Abstract<p>Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads progress to cortical layers to establish functional nodules. Here we use single-cell analysis to define the epidermal and cortical cell populations that respond to and facilitate rhizobium infection. We then identify high-confidence nodulation gene candidates based on their specific expression in these populations, pinpointing genes stably associated with infection across genotypes and time points. We show that one of these, which we name SYMRKL1, encodes a protein with an ectodomain predicted to be nearly identical to that of SYMRK and is required for normal infection thread formation. Our work disentangles cellular processes and transcriptional modules that were previously confounded due to lack of cellular resolution, providing a more detailed understanding of symbiotic interactions.</p", "keywords": ["Rhizobium/metabolism", "Science", "Q", "Plant Roots/metabolism", "Plant Proteins/genetics", "Plant Roots", "Article", "Root Nodules", " Plant/metabolism", "Symbiosis/genetics", "Phenotype", "Lotus/metabolism", "Gene Expression Regulation", " Plant", "Lotus", "Single-Cell Analysis", "Root Nodules", " Plant", "Symbiosis", "Rhizobium", "Plant Proteins"]}, "links": [{"href": "https://www.nature.com/articles/s41467-023-42911-1.pdf"}, {"href": "https://doi.org/PMC10630511"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC10630511", "name": "item", "description": "PMC10630511", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC10630511"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-11-07T00:00:00Z"}}, {"id": "PMC11449112", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:29:43Z", "type": "Journal Article", "created": "2024-07-16", "title": "Gibberellin dynamics governing nodulation revealed using GIBBERELLIN PERCEPTION SENSOR 2 in Medicago truncatula lateral organs", "description": "Abstract                <p>During nutrient scarcity, plants can adapt their developmental strategy to maximize their chance of survival. Such plasticity in development is underpinned by hormonal regulation, which mediates the relationship between environmental cues and developmental outputs. In legumes, endosymbiosis with nitrogen-fixing bacteria (rhizobia) is a key adaptation for supplying the plant with nitrogen in the form of ammonium. Rhizobia are housed in lateral root-derived organs termed nodules that maintain an environment conducive to Nitrogenase in these bacteria. Several phytohormones are important for regulating the formation of nodules, with both positive and negative roles proposed for gibberellin (GA). In this study, we determine the cellular location and function of bioactive GA during nodule organogenesis using a genetically encoded second-generation GA biosensor, GIBBERELLIN PERCEPTION SENSOR 2 in Medicago truncatula. We find endogenous bioactive GA accumulates locally at the site of nodule primordia, increasing dramatically in the cortical cell layers, persisting through cell divisions, and maintaining accumulation in the mature nodule meristem. We show, through misexpression of GA-catabolic enzymes that suppress GA accumulation, that GA acts as a positive regulator of nodule growth and development. Furthermore, increasing or decreasing GA through perturbation of biosynthesis gene expression can increase or decrease the size of nodules, respectively. This is unique from lateral root formation, a developmental program that shares common organogenesis regulators. We link GA to a wider gene regulatory program by showing that nodule-identity genes induce and sustain GA accumulation necessary for proper nodule formation.</p", "keywords": ["2. Zero hunger", "Plant Growth Regulators", "Gene Expression Regulation", " Plant", "Medicago truncatula", "Root Nodules", " Plant", "Plants", " Genetically Modified", "Plant Root Nodulation", "Plant Roots", "Gibberellins", "Research Article", "Plant Proteins"]}, "links": [{"href": "https://doi.org/PMC11449112"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Plant%20Cell", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11449112", "name": "item", "description": "PMC11449112", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11449112"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-07-16T00:00:00Z"}}, {"id": "PMC11637764", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:29:44Z", "type": "Journal Article", "created": "2024-08-28", "title": "Leaf Gas Film 1 promotes glycerol ester accumulation and formation of a tight root barrier to radial O2 loss in rice", "description": "Abstract                <p>Rice (Oryza sativa L.) and many other wetland plants form an apoplastic barrier in the outer parts of the roots to restrict radial O2 loss to the rhizosphere during soil flooding. This barrier facilitates longitudinal internal O2 diffusion via gas-filled tissues from shoot to root apices, enabling root growth in anoxic soils. We tested the hypothesis that Leaf Gas Film 1 (LGF1), which influences leaf hydrophobicity in rice, plays a crucial role in tight outer apoplastic barrier formation in rice roots. We examined the roots of a rice mutant (dripping wet leaf 7, drp7) lacking functional LGF1, its wild type, and an LGF1 overexpression line for their capacity to develop outer apoplastic barriers that restrict radial O2 loss. We quantified the chemical composition of the outer part of the root and measured radial O2 diffusion from intact roots. The drp7 mutant exhibited a weak barrier to radial O2 loss compared to the wild type. However, introducing functional LGF1 into the mutant fully restored tight barrier function. The formation of a tight barrier to radial O2 loss was associated with increased glycerol ester levels in exodermal cells, rather than differences in total root suberization or lignification. These results demonstrate that, in addition to its role in leaf hydrophobicity regulation, LGF1 plays an important role in controlling the function of the outer apoplastic barriers in roots. Our study suggests that increased deposition of glycerol esters in the suberized root exodermis establishes a tight barrier to radial O2 loss in rice roots.</p", "keywords": ["Oxygen", "Glycerol", "Plant Leaves", "0106 biological sciences", "2. Zero hunger", "Mutation", "Oryza", "Esters", "15. Life on land", "Plant Roots", "01 natural sciences", "Research Article", "Plant Proteins"]}, "links": [{"href": "https://academic.oup.com/plphys/article-pdf/196/4/2437/60933328/kiae458.pdf"}, {"href": "https://doi.org/PMC11637764"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Physiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11637764", "name": "item", "description": "PMC11637764", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11637764"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-28T00:00:00Z"}}, {"id": "PMC11839481", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:29:44Z", "type": "Journal Article", "created": "2025-01-15", "title": "Autoactive CNGC15 enhances root endosymbiosis in legume and wheat", "description": "Abstract           <p>Nutrient acquisition is crucial for sustaining life. Plants develop beneficial intracellular partnerships with arbuscular mycorrhiza (AM) and nitrogen-fixing bacteria to surmount the scarcity of soil nutrients and tap into atmospheric dinitrogen, respectively1,2. Initiation of these root endosymbioses requires symbiont-induced oscillations in nuclear calcium (Ca2+) concentrations in root cells3. How the nuclear-localized ion channels, cyclic nucleotide-gated channel (CNGC) 15 and DOESN\uffe2\uff80\uff99T MAKE INFECTIONS1 (DMI1)4 are coordinated to specify symbiotic-induced nuclear Ca2+ oscillations remains unknown. Here we discovered an autoactive CNGC15 mutant that generates spontaneous low-frequency Ca2+ oscillations. While CNGC15 produces nuclear Ca2+ oscillations via a gating mechanism involving its helix 1, DMI1 acts as a pacemaker to specify the frequency of the oscillations. We demonstrate that the specificity of symbiotic-induced nuclear Ca2+ oscillations is encoded in its frequency. A high frequency activates endosymbiosis programmes, whereas a low frequency modulates phenylpropanoid pathways. Consequently, the autoactive cngc15 mutant, which is capable of generating both frequencies, has increased flavonoids that enhance AM, root nodule symbiosis and nutrient acquisition. We transferred this trait to wheat, resulting in field-grown wheat with increased AM colonization and nutrient acquisition. Our findings reveal a new strategy to boost endosymbiosis in the field and reduce inorganic fertilizer use while sustaining plant growth.</p", "keywords": ["Cell Nucleus", "Mycorrhizae", "Mutation", "Medicago truncatula", "Cyclic Nucleotide-Gated Cation Channels", "Calcium", "Fabaceae", "Calcium Signaling", "Symbiosis", "Plant Roots", "Article", "Triticum", "Plant Proteins"]}, "links": [{"href": "https://doi.org/PMC11839481"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11839481", "name": "item", "description": "PMC11839481", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11839481"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-15T00:00:00Z"}}, {"id": "PMC6795825", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:29:47Z", "type": "Journal Article", "created": "2019-10-16", "title": "Phosphorus-acquisition strategies of canola, wheat and barley in soil amended with sewage sludges", "description": "Abstract<p>Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition strategies of three main crops are affected by the application of sewage sludges, compared with a mineral P fertilizer. We carried out a 3-months greenhouse pot experiment and compared the response of P-acquisition traits among wheat, barley and canola in a soil amended with three sludges or a mineral P fertilizer. Results showed that the P-acquisition strategy differed among crops. Compared with canola, wheat and barley had a higher specific root length and a greater root carboxylate release and they acquired as much P from sludge as from mineral P. By contrast, canola shoot P content was greater with sludge than with mineral P. This was attributed to a higher root-released acid phosphatase activity which promoted the mineralization of sludge-derived P-organic. This study showed that contrasted P-acquisition strategies of crops allows increased use of renewable P resources by optimizing combinations of crop and the type of P fertilizer applied within the cropping system.</p", "keywords": ["Calcium Phosphates", "Crops", " Agricultural", "0106 biological sciences", "phosphatase activity", "N\u00e4hrstoffaufnahme", "carboxylate", "Phytic Acid", "Acid Phosphatase", "[SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy", "Carboxylic Acids", "organic P fertilizer", " mineral P fertilizer", " carboxylate", " phosphatase activity", "Plant Roots", "01 natural sciences", "630", "Article", "12. Responsible consumption", "Soil", "Boden", "Species Specificity", "ddc:630", "Humans", "Fertilizers", "Triticum", "Plant Proteins", "2. Zero hunger", "Plant Stems", "Sewage", "Brassica rapa", "Agriculture", "Biological Transport", "Hordeum", "Phosphorus", "Phosphor", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "0401 agriculture", " forestry", " and fisheries", "G\u00e4rrest", "mineral P fertilizer", "organic P fertilizer"]}, "links": [{"href": "https://www.nature.com/articles/s41598-019-51204-x.pdf"}, {"href": "https://doi.org/PMC6795825"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC6795825", "name": "item", "description": "PMC6795825", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC6795825"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-16T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Plant+Proteins&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Plant+Proteins&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Plant+Proteins&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Plant+Proteins&offset=50", "hreflang": "en-US"}], "numberMatched": 51, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-06-23T22:48:37.486687Z"}