{"type": "FeatureCollection", "features": [{"id": "10.5281/zenodo.6380872", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:20:23Z", "type": "Journal Article", "title": "Optimization of a hemolymph protein extraction method from native polyacrylamide gel", "description": "<strong>INTRODUCTION:</strong> Although there are indications that insect-based proteins may have potential biomedical applications (anticancer and antimicrobial), as well as in cellular agriculture (food and feed), they have not been sufficiently investigated. The hemolymph of insect larvae is protein-rich, particularly in storage proteins that are involved in amino acid metabolism and protein synthesis. In order to characterize these proteins, the first step is their successful isolation. Using diapausing 5th instar larvae of the economically important European corn borer moth (ECB) <em>Ostrinia nubilalis </em>(Hbn.) as a model system, in this study we optimized a method for isolating individual native hemolymph proteins from polyacrylamide gels and we performed initial tests of isolated proteins bioactivity. <strong>OBJECTIVES:</strong> The main objective in this study was to optimize an easy and affordable method for isolation of individual hemolymph proteins in the native state, without the use of chemicals that would affect their structure and function (e.g. sodium dodecyl sulfate, SDS). This allows further testing of these proteins for biomedical and application in cellular agriculture, and further work with isolated proteins in downstream in vitro proteome research, which will bring new knowledge and directions for different in silico proteome research. <strong>METHOD / DESIGN: </strong>Hemolymph was collected from diapausing 5th instar ECB larvae, after which hemocytes were removed from the hemolymph by centrifuging the samples for 30 min. at 16 000 g. Hemolymph proteins were separated by native polyacrylamide gel electrophoresis (PAGE) on a customized discontinuous gel without a well comb, using the BIO-RAD Mini-PROTEAN\ufffd\ufffd Tetra cell. <br> In order to determine the position of protein fractions of interest on the gel after electrophoresis, thin vertical strips were cut from the sides of the polyacrylamide gel and stained with Coomassie Brilliant Blue, after which the same gel strips were destained. The strips were placed next to the original gels and 5 protein fractions were cut from the unstained part of the polyacrylamide gel, chopped and transferred to microtubes. Ultrapure water was added to the tubes and they were placed on the Biometra TSC ThermoShaker overnight at 30\ufffd\ufffdC to elute the proteins from the gels. After elution, the protein samples were centrifuged for 15 min. at 10 000 g. The concentration of isolated proteins was determined by measuring the absorbance at 230 nm using the Shimadzu BioSpec-nano, with a serial dilution of bovine \ufffd\ufffd-globulin used as the protein standard. To confirm that the proteins were well isolated, the individual fractions were run in duplicate wells on discontinuous native PAGE using the BIO-RAD Mini-PROTEAN\ufffd\ufffd 3 Cell, after which the gels were stained, destained and imaged. Finally, the effect of successfully isolated proteins on MRC-5 cell viability was examined using an MTT assay. <strong>RESULTS: </strong>Five distinct protein fractions were detected after the first native PAGE (P1-P5). After elution from the gel, these fractions and the method for their isolation were validated with a second native PAGE. Regarding the testing of isolated protein bioactivity, the results of the MTT assay indicate an antiproliferative effect of all 5 protein fractions, especially in the P4 fraction. <strong>CONCLUSIONS: </strong>The insect hemolymph protein extraction method optimized in this study proved to be simple and successful and could potentially be applied to other insect species as well. Also, the structure and function of the proteins remained intact during the isolation process, which allows further use of the isolated proteins in downstream in vitro proteome research, the results of which will contribute to protein identification and in silico proteome research based on different bioinformatics tools (e.g. protein-protein interaction analysis, in silico bioactivity analyses, etc.). Finally, since the isolated proteins showed antiproliferative effects on the selected cell line, their anticancer and antimicrobial activity will be further tested.", "keywords": ["insects; hemolymph proteins; native PAGE; protein isolation; proteomics"], "contacts": [{"organization": "Teodora, Kne\u017ei\u0107, Milo\u0161, Avramov, \u017deljko, Popovi\u0107 D., Ljiljana, Janju\u0161evi\u0107, Mila, Djisalov, Ivana, Gadjanski,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.6380872"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Book%20of%20Abstracts%20-%20Bioscience%20Conference%20and%20the%208th%20International%20PSU%20%E2%80%93%20UNS%20Bioscience%20Conference%20%28IBSC2021%29", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.6380872", "name": "item", "description": "10.5281/zenodo.6380872", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.6380872"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "10.1007/s00425-017-2647-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:14:33Z", "type": "Journal Article", "created": "2017-01-04", "title": "The cost of surviving nitrogen excess: energy and protein demand in the lichen Cladonia portentosa as revealed by proteomic analysis", "description": "Different nitrogen forms affect different metabolic pathways in lichens. In particular, the most relevant changes in protein expression were observed in the fungal partner, with NO 3- mostly affecting the energetic metabolism and NH 4+ affecting transport and regulation of proteins and the energetic metabolism much more than NO 3- did. Excess deposition of reactive nitrogen is a well-known agent of stress for lichens, but which symbiont is most affected and how, remains a mystery. Using proteomics can expand our understanding of stress effects on lichens. We investigated the effects of different doses and forms of reactive nitrogen, with and without supplementary phosphorus and potassium, on the proteome of the lichen Cladonia portentosa growing in a 'real-world' simulation of nitrogen deposition. Protein expression changed with the nitrogen treatments but mostly in the fungal partner, with NO3- mainly affecting the energetic metabolism and NH4+ also affecting the protein synthesis machinery. The photobiont mainly responded overexpressing proteins involved in energy production. This suggests that in response to nitrogen stress, the photobiont mainly supports the defensive mechanisms initiated by the mycobiont with an increased energy production. Such surplus energy is then used by the cell to maintain functionality in the presence of NO3-, while a futile cycle of protein production can be hypothesized to be induced by NH4+ excess. External supply of potassium and phosphorus influenced differently the responses of particular enzymes, likely reflecting the many processes in which potassium exerts a regulatory function.", "keywords": ["Chlorophyll", "Proteomics", "0301 basic medicine", "570", "mycobiont", "Lichens", "Nitrogen", "Cell Respiration", "Nitrate", "Mass Spectrometry", "Molecular mechanism", "03 medical and health sciences", "nitrate", "Ammonia", "Electrophoresis", " Gel", " Two-Dimensional", "Photosynthesis", "Ammonium", " Molecular mechanism", " Mycobiont", " Nitrate", " Photobiont", " Stress response", "Ammonium; Molecular mechanism; Mycobiont; Nitrate; Photobiont; Stress response; Genetics; Plant Science", "0303 health sciences", "Nitrates", "Stress response", "Chlorophyll A", "stress response", "Mycobiont", "ammonium", "Photobiont", "photobiont", "molecular mechanism", "Energy Metabolism", "Ammonium"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00425-017-2647-2.pdf"}, {"href": "https://doi.org/10.1007/s00425-017-2647-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Planta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00425-017-2647-2", "name": "item", "description": "10.1007/s00425-017-2647-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00425-017-2647-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-04T00:00:00Z"}}, {"id": "10.1016/j.ecolind.2020.106817", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:15:26Z", "type": "Journal Article", "created": "2020-08-10", "title": "More tolerant than expected: Taking into account the ability of Cladonia portentosa to cope with increased nitrogen availability in environmental policy", "description": "Abstract   The lichen Cladonia portentosa is generally considered to be sensitive to increased environmental nitrogen (N) deposition. However, the presence of this lichen in impacted environments suggests that it can cope with prolonged exposure to high N availability. To test the tolerance of this species to N, photosynthetic parameters, carbon and N concentrations and isotopic signature, chitin concentration, surface pH and extracellular enzymatic activity were measured in samples exposed for 11\u00a0years to different N doses and forms at the Whim bog N manipulation experimental site (United Kingdom). The results showed that C. portentosa is tolerant to long-term exposure to wet N deposition, maintaining its functionality with almost unaltered physiological parameters. The comparison of the proteome of short- and long-term exposed samples showed similar changes in protein expression suggesting that mechanisms to cope with N are not dependent on the exposure time even after more than a decade. Since empirical N Critical Loads are based on the response of sensitive components of the ecosystem, like C. portentosa, its capacity to cope with short- and long-term exposure to N needs to be recognized and taken into account when setting them, likewise, the significance of the form of N.    Capsule  Updated knowledge on tolerance of sensitive species to nitrogen must be taken into account to establish environmental policy.", "keywords": ["Critical loads and levels", "Proteomics", "0106 biological sciences", "570", "long-term exposure", "Long-term exposure", "Algal ultrastructure; Critical loads and levels; Long-term exposure; Physiological response; Photosynthetic parameters; Proteomics", "algal ultrastructure", "Photosynthetic parameter", "15. Life on land", "01 natural sciences", "critical loads and levels", "Algal ultrastructure", "photosynthetic parameters. proteomics", "Algal ultrastructure; Critical loads and levels; Long-term exposure; Photosynthetic parameters; Physiological response; Proteomics", "Physiological response", "Photosynthetic parameters", "13. Climate action", "Critical loads and level", "physiological response", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://cris.unibo.it/bitstream/11585/772327/7/More%20tolerant.pdf"}, {"href": "https://doi.org/10.1016/j.ecolind.2020.106817"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecological%20Indicators", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ecolind.2020.106817", "name": "item", "description": "10.1016/j.ecolind.2020.106817", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ecolind.2020.106817"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.1038/srep15550", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:16:40Z", "type": "Journal Article", "created": "2015-10-27", "title": "Soil Restoration With Organic Amendments: Linking Cellular Functionality And Ecosystem Processes", "description": "Abstract<p>A hot topic in recent decades, the application of organic amendments to arid-degraded soils has been shown to benefit microbially-mediated processes. However, despite the importance of soils for global sustainability, a gap has not been addressed yet in soil science: is there any connection between ecosystem-community processes, cellular functionality and microbial lifestyles (i.e. oligotrophy-copiotrophy) in restored soils? Together with classical ecosystem indicators (fatty-acids, extracellular-enzyme activities, basal respiration), state-of-the-art metaproteomics was applied to fill this gap in a model-restoration experiment initiated 10-years ago by the addition of sewage-sludge and compost. Organic amendment strongly impacted ecosystem processes. Furthermore, the type of material used induced differences in the cellular functionalities through variations in the percentages of proteins involved in translation, transcription, energy production and C-fixation. We conclude that the long-term impact of organic restoration goes beyond ecosystem processes and affects cellular functionalities and phyla-lifestyles coupled with differences in microbial-community structures.</p>", "keywords": ["Proteomics", "2. Zero hunger", "0301 basic medicine", "1000 Multidisciplinary", "Sewage", "610 Medicine & health", "10071 Functional Genomics Center Zurich", "04 agricultural and veterinary sciences", "15. Life on land", "Article", "6. Clean water", "Enzymes", "Environmental sciences", "Soil", "03 medical and health sciences", "Soil microbiology", "13. Climate action", "11. Sustainability", "570 Life sciences; biology", "0401 agriculture", " forestry", " and fisheries", "Soil microbiology; Environmental sciences", "Ecosystem", "Environmental Restoration and Remediation", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1038/srep15550"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/srep15550", "name": "item", "description": "10.1038/srep15550", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/srep15550"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-10-27T00:00:00Z"}}, {"id": "10.1038/s41467-024-51515-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:16:36Z", "type": "Journal Article", "created": "2024-08-23", "title": "A NAC triad modulates plant immunity by negatively regulating N-hydroxy pipecolic acid biosynthesis", "description": "N-hydroxy\u00a0pipecolic acid (NHP) plays an important role in plant immunity. In contrast to its biosynthesis, our current knowledge with respect to the transcriptional regulation of the NHP pathway is limited. This study commences with the engineering of Arabidopsis plants that constitutively produce high NHP levels and display enhanced immunity. Label-free proteomics reveals a NAC-type transcription factor (NAC90) that is strongly induced in these plants. We find that NAC90 is a target gene of SAR DEFICIENT 1 (SARD1) and induced by pathogen, salicylic acid (SA), and NHP. NAC90 knockout mutants exhibit constitutive immune activation, earlier senescence, higher levels of NHP and SA, as well as increased expression of NHP and SA biosynthetic genes. In contrast, NAC90 overexpression lines are compromised in disease resistance and accumulated reduced levels of NHP and SA. NAC90 could interact with NAC61 and NAC36 which are also induced by pathogen, SA, and NHP. We next discover that this protein triad directly represses expression of the NHP and SA biosynthetic genes AGD2-LIKE DEFENSE RESPONSE PROTEIN 1 (ALD1), FLAVIN MONOOXYGENASE 1 (FMO1), and ISOCHORISMATE SYNTHASE 1 (ICS1). Constitutive immune response in nac90 is abolished once blocking NHP biosynthesis in the fmo1 background, signifying that NAC90 negative regulation of immunity is mediated via NHP biosynthesis. Our findings expand the currently documented NHP regulatory network suggesting a model that together with NHP glycosylation, NAC repressors take part in a 'gas-and-brake' transcriptional mechanism to control NHP production and the plant growth and defense trade-off.", "keywords": ["Proteomics", "0301 basic medicine", "0303 health sciences", "Arabidopsis Proteins", "Science", "Q", "Arabidopsis", "Plants", " Genetically Modified", "Article", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Pipecolic Acids", "Plant Immunity", "Salicylic Acid", "Transcription Factors", "Plant Diseases", "Disease Resistance"]}, "links": [{"href": "https://doi.org/10.1038/s41467-024-51515-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-024-51515-2", "name": "item", "description": "10.1038/s41467-024-51515-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-024-51515-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-22T00:00:00Z"}}, {"id": "10.1093/jxb/erq249", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:17:03Z", "type": "Journal Article", "created": "2010-08-27", "title": "Plant Physiology And Proteomics Reveals The Leaf Response To Drought In Alfalfa (Medicago Sativa L.)", "description": "Despite its relevance, protein regulation, metabolic adjustment, and the physiological status of plants under drought is not well understood in relation to the role of nitrogen fixation in nodules. In this study, nodulated alfalfa plants were exposed to drought conditions. The study determined the physiological, metabolic, and proteomic processes involved in photosynthetic inhibition in relation to the decrease in nitrogenase (N(ase)) activity. The deleterious effect of drought on alfalfa performance was targeted towards photosynthesis and N(ase) activity. At the leaf level, photosynthetic inhibition was mainly caused by the inhibition of Rubisco. The proteomic profile and physiological measurements revealed that the reduced carboxylation capacity of droughted plants was related to limitations in Rubisco protein content, activation state, and RuBP regeneration. Drought also decreased amino acid content such as asparagine, and glutamic acid, and Rubisco protein content indicating that N availability limitations were caused by N(ase) activity inhibition. In this context, drought induced the decrease in Rubisco binding protein content at the leaf level and proteases were up-regulated so as to degrade Rubisco protein. This degradation enabled the reallocation of the Rubisco-derived N to the synthesis of amino acids with osmoregulant capacity. Rubisco degradation under drought conditions was induced so as to remobilize Rubisco-derived N to compensate for the decrease in N associated with N(ase) inhibition. Metabolic analyses showed that droughted plants increased amino acid (proline, a major compound involved in osmotic regulation) and soluble sugar (D-pinitol) levels to contribute towards the decrease in osmotic potential (\u03a8(s)). At the nodule level, drought had an inhibitory effect on N(ase) activity. This decrease in N(ase) activity was not induced by substrate shortage, as reflected by an increase in total soluble sugars (TSS) in the nodules. Proline accumulation in the nodule could also be associated with an osmoregulatory response to drought and might function as a protective agent against ROS. In droughted nodules, the decrease in N(2) fixation was caused by an increase in oxygen resistance that was induced in the nodule. This was a mechanism to avoid oxidative damage associated with reduced respiration activity and the consequent increase in oxygen content. This study highlighted that even though drought had a direct effect on leaves, the deleterious effects of drought on nodules also conditioned leaf responsiveness.", "keywords": ["Proteomics", "0301 basic medicine", "570", "Rubisco", "Proteome", "[SDV]Life Sciences [q-bio]", "proteome", "N-2 FIXATION RESPONSE", "drought", "N2 fixation", "03 medical and health sciences", "XANTHOPHYLL CYCLE", "N-2 fixation", "2-CYSTEINE PEROXIREDOXIN", "Nitrogenase", "oxidative stress", "AMINO-ACIDS", "Photosynthesis", "climate", "agriculture", "Plant Proteins", "580", "N remobilization", "2. Zero hunger", "0303 health sciences", "photosynthesis", "PINITOL ACCUMULATION", "Drought", "RIBULOSE-1", "5-BISPHOSPHATE CARBOXYLASE-OXYGENASE", "Water", "BRASSICA-NAPUS", "N(O)-TERT-BUTYLDIMETHYLSILYL DERIVATIVES", "15. Life on land", "Research Papers", "6. Clean water", "Droughts", "[SDV] Life Sciences [q-bio]", "Plant Leaves", "nitrogen fixation", "Oxidative stress", "rubisco", "NITROGEN-FIXATION", "WATER-WATER CYCLE", "Medicago sativa"]}, "links": [{"href": "https://doi.org/10.1093/jxb/erq249"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jxb/erq249", "name": "item", "description": "10.1093/jxb/erq249", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jxb/erq249"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-25T00:00:00Z"}}, {"id": "10.1093/ismeco/ycae116", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:17:02Z", "type": "Journal Article", "created": "2024-10-08", "title": "Land use effects on soil microbiome composition and traits with consequences for soil carbon cycling", "description": "Abstract                <p>The soil microbiome determines the fate of plant-fixed carbon. The shifts in soil properties caused by land use change leads to modifications in microbiome function, resulting in either loss or gain of soil organic carbon (SOC). Soil pH is the primary factor regulating microbiome characteristics leading to distinct pathways of microbial carbon cycling, but the underlying mechanisms remain understudied. Here, the taxa-trait relationships behind the variable fate of SOC were investigated using metaproteomics, metabarcoding, and a 13C-labeled litter decomposition experiment across two temperate sites with differing soil pH each with a paired land use intensity contrast. 13C incorporation into microbial biomass increased with land use intensification in low-pH soil but decreased in high-pH soil, with potential impact on carbon use efficiency in opposing directions. Reduction in biosynthesis traits was due to increased abundance of proteins linked to resource acquisition and stress tolerance. These trait trade-offs were underpinned by land use intensification-induced changes in dominant taxa with distinct traits. We observed divergent pH-controlled pathways of SOC cycling. In low-pH soil, land use intensification alleviates microbial abiotic stress resulting in increased biomass production but promotes decomposition and SOC loss. In contrast, in high-pH soil, land use intensification increases microbial physiological constraints and decreases biomass production, leading to reduced necromass build-up and SOC stabilization. We demonstrate how microbial biomass production and respiration dynamics and therefore carbon use efficiency can be decoupled from SOC highlighting the need for its careful consideration in managing SOC storage for soil health and climate change mitigation.</p", "keywords": ["soil health", "Supplementary Data", "QH301 Biology", "carbon use efficiency", "carbon cycling", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_16s_table_s1_ycae116.xlsx?Expires=1737538557&Signature=3IutEpMaJIknJFjSbheOQYWpAwXt2atlN4YtPR7BTaTGf3jrf1M6yHgYzlnrttKlwpbFcwz-IqYq96oubC5FxfBQQyiIC0H-az-D~Bkstxc9XHkEmERELO~nurTlszmUndzm3jLsKF05x00PNsiNFlGKUhlsMB6wRmyO3v3GNBqHQVdswXZ3UAjfXvqqinyDLK54UCxfLk8eKpcfFnvVctxQ8Hrk3gP-eMFToKDlXgPD4MXGrdegvcZblx6g8FAvJruLIG1NWIRJ6wzx6HcmAYiZDJcGosKrdjMBIznM8YIJjBrfWwhGvjh15Z7MJnsUWn8PjxLjXfww29q-YfQnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_18s_table_s2_ycae116.xlsx?Expires=1737538557&Signature=ZVWC9BaJ2MOsxOOfzrmd-9nuLAy5yHOmeqJQmKHhQ1z7mXxXITIYAvM8BpVkEkQHB7Bo-6dNEm5FlC6eAuTroyq-dvMW3PD6MNP9SN5KgwSrKUeHM6IKNhzav6Q4zd48B95IPreN5UKQTTVPrphpdOxfdVKYKxD3qOMdWqmHXt-IAD~W80PJ0BjvpHXPQ0pYCmGInVv1Fe-L3k~OKo80rD0xtncnBCFRd8DVHTIY5JLjJr4-E~M3Gainkbz2AVLZwys3S6MMEboS8vKSj~rG34Z04ByT6dBjp0XDj2H9K7WjXlEqOoPIwUWUUfcVvn4N5wZ6R6YFZr9mk4qTZKdEow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "004", "soil organic carbon", "QH301", "soil pH", "13C labelling", "land use intensity", "soil microbiome", "metabarcoding", "SDG 13 - Climate Action", "metaproteomics", "Original Article", "SDG 15 - Life on Land"]}, "links": [{"href": "https://doi.org/10.1093/ismeco/ycae116"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ISME%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/ismeco/ycae116", "name": "item", "description": "10.1093/ismeco/ycae116", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/ismeco/ycae116"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2016.01247", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:18:36Z", "type": "Journal Article", "created": "2016-08-08", "title": "Soil Fungal:Bacterial Ratios Are Linked to Altered Carbon Cycling", "description": "Despite several lines of observational evidence, there is a lack of consensus on whether higher fungal:bacterial (F:B) ratios directly cause higher soil carbon (C) storage. We employed RNA sequencing, protein profiling and isotope tracer techniques to evaluate whether differing F:B ratios are associated with differences in C storage. A mesocosm (13)C labeled foliar litter decomposition experiment was performed in two soils that were similar in their physico-chemical properties but differed in microbial community structure, specifically their F:B ratio (determined by PLFA analyses, RNA sequencing and protein profiling; all three corroborating each other). Following litter addition, we observed a consistent increase in abundance of fungal phyla; and greater increases in the fungal dominated soil; implicating the role of fungi in litter decomposition. Litter derived (13)C in respired CO2 was consistently lower, and residual (13)C in bulk SOM was higher in high F:B soil demonstrating greater C storage potential in the F:B dominated soil. We conclude that in this soil system, the increased abundance of fungi in both soils and the altered C cycling patterns in the F:B dominated soils highlight the significant role of fungi in litter decomposition and indicate that F:B ratios are linked to higher C storage potential.", "keywords": ["Microbiology (medical)", "Proteomics", "0301 basic medicine", "environment/Bioclimatology", "Supplementary Data", "[SDE.MCG]Environmental Sciences/Global Changes", "stable isotopes", "litter decomposition", "Microbiology", "03 medical and health sciences", "proteomics", "[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "[SDV.EE]Life Sciences [q-bio]/Ecology", "[SDU.STU.GC] Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "soil carbon", "European Commission", "bacteria", "Stable isotopes", "2. Zero hunger", "655240", "0303 health sciences", "Bacteria", "Litter decomposition", "Fungi", "RNA sequencing", "QR Microbiology", "15. Life on land", "Soil carbon", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "QR1-502", "6. Clean water", "QR", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "[SDE.MCG] Environmental Sciences/Global Changes", "[SDV.EE] Life Sciences [q-bio]/Ecology", " environment", "[SDV.EE.BIO] Life Sciences [q-bio]/Ecology", " environment/Bioclimatology", "[SDV.EE.BIO]Life Sciences [q-bio]/Ecology", "fungi", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "environment"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2016.01247"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.01247", "name": "item", "description": "10.3389/fmicb.2016.01247", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.01247"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-08-09T00:00:00Z"}}, {"id": "11585/772327", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-19T16:21:25Z", "type": "Journal Article", "created": "2020-08-10", "title": "More tolerant than expected: Taking into account the ability of Cladonia portentosa to cope with increased nitrogen availability in environmental policy", "description": "Abstract   The lichen Cladonia portentosa is generally considered to be sensitive to increased environmental nitrogen (N) deposition. However, the presence of this lichen in impacted environments suggests that it can cope with prolonged exposure to high N availability. To test the tolerance of this species to N, photosynthetic parameters, carbon and N concentrations and isotopic signature, chitin concentration, surface pH and extracellular enzymatic activity were measured in samples exposed for 11\u00a0years to different N doses and forms at the Whim bog N manipulation experimental site (United Kingdom). The results showed that C. portentosa is tolerant to long-term exposure to wet N deposition, maintaining its functionality with almost unaltered physiological parameters. The comparison of the proteome of short- and long-term exposed samples showed similar changes in protein expression suggesting that mechanisms to cope with N are not dependent on the exposure time even after more than a decade. Since empirical N Critical Loads are based on the response of sensitive components of the ecosystem, like C. portentosa, its capacity to cope with short- and long-term exposure to N needs to be recognized and taken into account when setting them, likewise, the significance of the form of N.    Capsule  Updated knowledge on tolerance of sensitive species to nitrogen must be taken into account to establish environmental policy.", "keywords": ["Critical loads and levels", "Proteomics", "0106 biological sciences", "570", "long-term exposure", "Long-term exposure", "Algal ultrastructure; Critical loads and levels; Long-term exposure; Physiological response; Photosynthetic parameters; Proteomics", "algal ultrastructure", "Photosynthetic parameter", "15. Life on land", "01 natural sciences", "critical loads and levels", "Algal ultrastructure", "photosynthetic parameters. proteomics", "Algal ultrastructure; Critical loads and levels; Long-term exposure; Photosynthetic parameters; Physiological response; Proteomics", "Physiological response", "Photosynthetic parameters", "13. Climate action", "Critical loads and level", "physiological response", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://repositorio.ulisboa.pt/bitstream/10451/45347/1/Munzi%20et%20al%202020.pdf"}, {"href": "https://cris.unibo.it/bitstream/11585/772327/7/More%20tolerant.pdf"}, {"href": "https://doi.org/11585/772327"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecological%20Indicators", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11585/772327", "name": "item", "description": "11585/772327", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11585/772327"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.5281/zenodo.6380873", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-19T16:20:23Z", "type": "Journal Article", "title": "Optimization of a hemolymph protein extraction method from native polyacrylamide gel", "description": "<strong>INTRODUCTION:</strong> Although there are indications that insect-based proteins may have potential biomedical applications (anticancer and antimicrobial), as well as in cellular agriculture (food and feed), they have not been sufficiently investigated. The hemolymph of insect larvae is protein-rich, particularly in storage proteins that are involved in amino acid metabolism and protein synthesis. In order to characterize these proteins, the first step is their successful isolation. Using diapausing 5th instar larvae of the economically important European corn borer moth (ECB) <em>Ostrinia nubilalis </em>(Hbn.) as a model system, in this study we optimized a method for isolating individual native hemolymph proteins from polyacrylamide gels and we performed initial tests of isolated proteins bioactivity. <strong>OBJECTIVES:</strong> The main objective in this study was to optimize an easy and affordable method for isolation of individual hemolymph proteins in the native state, without the use of chemicals that would affect their structure and function (e.g. sodium dodecyl sulfate, SDS). This allows further testing of these proteins for biomedical and application in cellular agriculture, and further work with isolated proteins in downstream in vitro proteome research, which will bring new knowledge and directions for different in silico proteome research. <strong>METHOD / DESIGN: </strong>Hemolymph was collected from diapausing 5th instar ECB larvae, after which hemocytes were removed from the hemolymph by centrifuging the samples for 30 min. at 16 000 g. Hemolymph proteins were separated by native polyacrylamide gel electrophoresis (PAGE) on a customized discontinuous gel without a well comb, using the BIO-RAD Mini-PROTEAN\ufffd\ufffd Tetra cell. <br> In order to determine the position of protein fractions of interest on the gel after electrophoresis, thin vertical strips were cut from the sides of the polyacrylamide gel and stained with Coomassie Brilliant Blue, after which the same gel strips were destained. The strips were placed next to the original gels and 5 protein fractions were cut from the unstained part of the polyacrylamide gel, chopped and transferred to microtubes. Ultrapure water was added to the tubes and they were placed on the Biometra TSC ThermoShaker overnight at 30\ufffd\ufffdC to elute the proteins from the gels. After elution, the protein samples were centrifuged for 15 min. at 10 000 g. The concentration of isolated proteins was determined by measuring the absorbance at 230 nm using the Shimadzu BioSpec-nano, with a serial dilution of bovine \ufffd\ufffd-globulin used as the protein standard. To confirm that the proteins were well isolated, the individual fractions were run in duplicate wells on discontinuous native PAGE using the BIO-RAD Mini-PROTEAN\ufffd\ufffd 3 Cell, after which the gels were stained, destained and imaged. Finally, the effect of successfully isolated proteins on MRC-5 cell viability was examined using an MTT assay. <strong>RESULTS: </strong>Five distinct protein fractions were detected after the first native PAGE (P1-P5). After elution from the gel, these fractions and the method for their isolation were validated with a second native PAGE. Regarding the testing of isolated protein bioactivity, the results of the MTT assay indicate an antiproliferative effect of all 5 protein fractions, especially in the P4 fraction. <strong>CONCLUSIONS: </strong>The insect hemolymph protein extraction method optimized in this study proved to be simple and successful and could potentially be applied to other insect species as well. Also, the structure and function of the proteins remained intact during the isolation process, which allows further use of the isolated proteins in downstream in vitro proteome research, the results of which will contribute to protein identification and in silico proteome research based on different bioinformatics tools (e.g. protein-protein interaction analysis, in silico bioactivity analyses, etc.). Finally, since the isolated proteins showed antiproliferative effects on the selected cell line, their anticancer and antimicrobial activity will be further tested.", "keywords": ["insects; hemolymph proteins; native PAGE; protein isolation; proteomics"], "contacts": [{"organization": "Teodora, Kne\u017ei\u0107, Milo\u0161, Avramov, \u017deljko, Popovi\u0107 D., Ljiljana, Janju\u0161evi\u0107, Mila, Djisalov, Ivana, Gadjanski,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.6380873"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Book%20of%20Abstracts%20-%20Bioscience%20Conference%20and%20the%208th%20International%20PSU%20%E2%80%93%20UNS%20Bioscience%20Conference%20%28IBSC2021%29", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.6380873", "name": "item", "description": "10.5281/zenodo.6380873", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.6380873"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "11585/582157", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:21:25Z", "type": "Journal Article", "created": "2017-01-04", "title": "The cost of surviving nitrogen excess: energy and protein demand in the lichen Cladonia portentosa as revealed by proteomic analysis", "description": "Different nitrogen forms affect different metabolic pathways in lichens. In particular, the most relevant changes in protein expression were observed in the fungal partner, with NO 3- mostly affecting the energetic metabolism and NH 4+ affecting transport and regulation of proteins and the energetic metabolism much more than NO 3- did. Excess deposition of reactive nitrogen is a well-known agent of stress for lichens, but which symbiont is most affected and how, remains a mystery. Using proteomics can expand our understanding of stress effects on lichens. We investigated the effects of different doses and forms of reactive nitrogen, with and without supplementary phosphorus and potassium, on the proteome of the lichen Cladonia portentosa growing in a 'real-world' simulation of nitrogen deposition. Protein expression changed with the nitrogen treatments but mostly in the fungal partner, with NO3- mainly affecting the energetic metabolism and NH4+ also affecting the protein synthesis machinery. The photobiont mainly responded overexpressing proteins involved in energy production. This suggests that in response to nitrogen stress, the photobiont mainly supports the defensive mechanisms initiated by the mycobiont with an increased energy production. Such surplus energy is then used by the cell to maintain functionality in the presence of NO3-, while a futile cycle of protein production can be hypothesized to be induced by NH4+ excess. External supply of potassium and phosphorus influenced differently the responses of particular enzymes, likely reflecting the many processes in which potassium exerts a regulatory function.", "keywords": ["Chlorophyll", "Proteomics", "0301 basic medicine", "570", "mycobiont", "Lichens", "Nitrogen", "Cell Respiration", "Nitrate", "Mass Spectrometry", "Molecular mechanism", "03 medical and health sciences", "nitrate", "Ammonia", "Electrophoresis", " Gel", " Two-Dimensional", "Photosynthesis", "Ammonium", " Molecular mechanism", " Mycobiont", " Nitrate", " Photobiont", " Stress response", "Ammonium; Molecular mechanism; Mycobiont; Nitrate; Photobiont; Stress response; Genetics; Plant Science", "0303 health sciences", "Nitrates", "Stress response", "Chlorophyll A", "stress response", "Mycobiont", "ammonium", "Photobiont", "photobiont", "molecular mechanism", "Energy Metabolism", "Ammonium"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00425-017-2647-2.pdf"}, {"href": "https://doi.org/11585/582157"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Planta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11585/582157", "name": "item", "description": "11585/582157", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11585/582157"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-04T00:00:00Z"}}, {"id": "1959.7/uws:65088", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-19T16:21:34Z", "type": "Journal Article", "created": "2021-06-04", "title": "Structure and function of bacterial metaproteomes across biomes", "description": "Abstract   Soil microbes, and the proteins they produce, are responsible for a myriad of soil processes which are integral to life on Earth, supporting soil fertility, nutrient fluxes, trace gas emissions, and plant production. However, how and why the composition of soil microbial proteins (the metaproteome) changes across wide gradients of vegetation, climatic and edaphic conditions remains largely undetermined. By applying high-resolution mass spectrometry to soil samples collected from four continents, we identified the most common proteins in soils, and investigated the primary environmental factors driving their distributions across climate and vegetation types. We found that soil proteins involved in carbohydrate metabolism, DNA repair, lipid metabolism, transcription regulation, tricarboxylic acid cycling, nitrogen (N) fixation and one-carbon metabolism dominate soils across a wide range of climates, vegetation types and edaphic conditions. Vegetation type and climate were important factors determining the community composition of the topsoil metaproteome. Moreover, we show that vegetation type, climate, and key edaphic proporties (mainly soil C fractions, pH and texture) influenced the proportion of important proteins involved in biogeochemical cycles and cellular processes. We also found that protein-based taxonomic information based on proteins has a greater resolution than 16S rRNA gene sequencing with regards to the ability to detect significant correlations with environmental variables. Together, our work identifies the dominant proteins produced by microbes living in a wide range of soils, and advances our understanding of how environmental changes can influence the structure and function of the topsoil metaproteome and the soil processes that they support.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Vegetation", "Metaproteomics", "13. Climate action", "Climate", "XXXXXX - Unknown", "Biodiversity", "15. Life on land", "Functionality", "Bacterial communities"]}, "links": [{"href": "https://doi.org/1959.7/uws:65088"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1959.7/uws:65088", "name": "item", "description": "1959.7/uws:65088", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1959.7/uws:65088"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-09-01T00:00:00Z"}}, {"id": "2164/13228", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:21:48Z", "type": "Journal Article", "created": "2016-08-08", "title": "Soil Fungal:Bacterial Ratios Are Linked to Altered Carbon Cycling", "description": "Despite several lines of observational evidence, there is a lack of consensus on whether higher fungal:bacterial (F:B) ratios directly cause higher soil carbon (C) storage. We employed RNA sequencing, protein profiling and isotope tracer techniques to evaluate whether differing F:B ratios are associated with differences in C storage. A mesocosm (13)C labeled foliar litter decomposition experiment was performed in two soils that were similar in their physico-chemical properties but differed in microbial community structure, specifically their F:B ratio (determined by PLFA analyses, RNA sequencing and protein profiling; all three corroborating each other). Following litter addition, we observed a consistent increase in abundance of fungal phyla; and greater increases in the fungal dominated soil; implicating the role of fungi in litter decomposition. Litter derived (13)C in respired CO2 was consistently lower, and residual (13)C in bulk SOM was higher in high F:B soil demonstrating greater C storage potential in the F:B dominated soil. We conclude that in this soil system, the increased abundance of fungi in both soils and the altered C cycling patterns in the F:B dominated soils highlight the significant role of fungi in litter decomposition and indicate that F:B ratios are linked to higher C storage potential.", "keywords": ["Microbiology (medical)", "Proteomics", "0301 basic medicine", "environment/Bioclimatology", "Supplementary Data", "[SDE.MCG]Environmental Sciences/Global Changes", "stable isotopes", "litter decomposition", "Microbiology", "03 medical and health sciences", "proteomics", "[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "[SDV.EE]Life Sciences [q-bio]/Ecology", "[SDU.STU.GC] Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "soil carbon", "European Commission", "bacteria", "Stable isotopes", "2. Zero hunger", "655240", "0303 health sciences", "Bacteria", "Litter decomposition", "Fungi", "RNA sequencing", "QR Microbiology", "15. Life on land", "Soil carbon", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "QR1-502", "6. Clean water", "QR", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "[SDE.MCG] Environmental Sciences/Global Changes", "[SDV.EE] Life Sciences [q-bio]/Ecology", " environment", "[SDV.EE.BIO] Life Sciences [q-bio]/Ecology", " environment/Bioclimatology", "[SDV.EE.BIO]Life Sciences [q-bio]/Ecology", "fungi", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "environment"]}, "links": [{"href": "https://doi.org/2164/13228"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2164/13228", "name": "item", "description": "2164/13228", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2164/13228"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-08-09T00:00:00Z"}}, {"id": "2164/24787", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:21:48Z", "type": "Journal Article", "created": "2024-10-08", "title": "Land use effects on soil microbiome composition and traits with consequences for soil carbon cycling", "description": "Abstract                <p>The soil microbiome determines the fate of plant-fixed carbon. The shifts in soil properties caused by land use change leads to modifications in microbiome function, resulting in either loss or gain of soil organic carbon (SOC). Soil pH is the primary factor regulating microbiome characteristics leading to distinct pathways of microbial carbon cycling, but the underlying mechanisms remain understudied. Here, the taxa-trait relationships behind the variable fate of SOC were investigated using metaproteomics, metabarcoding, and a 13C-labeled litter decomposition experiment across two temperate sites with differing soil pH each with a paired land use intensity contrast. 13C incorporation into microbial biomass increased with land use intensification in low-pH soil but decreased in high-pH soil, with potential impact on carbon use efficiency in opposing directions. Reduction in biosynthesis traits was due to increased abundance of proteins linked to resource acquisition and stress tolerance. These trait trade-offs were underpinned by land use intensification-induced changes in dominant taxa with distinct traits. We observed divergent pH-controlled pathways of SOC cycling. In low-pH soil, land use intensification alleviates microbial abiotic stress resulting in increased biomass production but promotes decomposition and SOC loss. In contrast, in high-pH soil, land use intensification increases microbial physiological constraints and decreases biomass production, leading to reduced necromass build-up and SOC stabilization. We demonstrate how microbial biomass production and respiration dynamics and therefore carbon use efficiency can be decoupled from SOC highlighting the need for its careful consideration in managing SOC storage for soil health and climate change mitigation.</p", "keywords": ["soil health", "Supplementary Data", "QH301 Biology", "carbon use efficiency", "carbon cycling", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_16s_table_s1_ycae116.xlsx?Expires=1737538557&Signature=3IutEpMaJIknJFjSbheOQYWpAwXt2atlN4YtPR7BTaTGf3jrf1M6yHgYzlnrttKlwpbFcwz-IqYq96oubC5FxfBQQyiIC0H-az-D~Bkstxc9XHkEmERELO~nurTlszmUndzm3jLsKF05x00PNsiNFlGKUhlsMB6wRmyO3v3GNBqHQVdswXZ3UAjfXvqqinyDLK54UCxfLk8eKpcfFnvVctxQ8Hrk3gP-eMFToKDlXgPD4MXGrdegvcZblx6g8FAvJruLIG1NWIRJ6wzx6HcmAYiZDJcGosKrdjMBIznM8YIJjBrfWwhGvjh15Z7MJnsUWn8PjxLjXfww29q-YfQnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_18s_table_s2_ycae116.xlsx?Expires=1737538557&Signature=ZVWC9BaJ2MOsxOOfzrmd-9nuLAy5yHOmeqJQmKHhQ1z7mXxXITIYAvM8BpVkEkQHB7Bo-6dNEm5FlC6eAuTroyq-dvMW3PD6MNP9SN5KgwSrKUeHM6IKNhzav6Q4zd48B95IPreN5UKQTTVPrphpdOxfdVKYKxD3qOMdWqmHXt-IAD~W80PJ0BjvpHXPQ0pYCmGInVv1Fe-L3k~OKo80rD0xtncnBCFRd8DVHTIY5JLjJr4-E~M3Gainkbz2AVLZwys3S6MMEboS8vKSj~rG34Z04ByT6dBjp0XDj2H9K7WjXlEqOoPIwUWUUfcVvn4N5wZ6R6YFZr9mk4qTZKdEow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "004", "soil organic carbon", "QH301", "soil pH", "13C labelling", "land use intensity", "soil microbiome", "metabarcoding", "SDG 13 - Climate Action", "metaproteomics", "Original Article", "SDG 15 - Life on Land"]}, "links": [{"href": "https://doi.org/2164/24787"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ISME%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2164/24787", "name": "item", "description": "2164/24787", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2164/24787"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "4c5838d44c1facdb93f19f10892f5240", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-09-19T16:22:33Z", "type": "Dataset", "title": "Species identification of archaeological skin objects from Danish bogs", "description": "Denmark has an extraordinarily large and well-preserved collection of archaeological skin garments found in peat bogs, dated to approximately 920 BC - AD 775. These objects provide not only the possibility to study prehistoric skin costume and technologies, but also to investigate the animal species used for production of skin garments. Until recently, species identification of archaeological skin was mainly performed by light and scanning electron microscopy or analysis of ancient DNA. However, the efficacy of these methods can be limited due to the harsh, mostly acidic environment of peat bogs leading to morphological and molecular degradation within the samples. We compared species assignment results of twelve archaeological skin samples from Danish bogs using Mass Spectrometry (MS)-based peptide sequencing, against results obtained using light and scanning electron microscopy", "keywords": ["Proteomics"]}, "links": [{"href": "https://doi.org/4c5838d44c1facdb93f19f10892f5240"}, {"rel": "self", "type": "application/geo+json", "title": "4c5838d44c1facdb93f19f10892f5240", "name": "item", "description": "4c5838d44c1facdb93f19f10892f5240", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/4c5838d44c1facdb93f19f10892f5240"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-07-29T00:00:00Z"}}, {"id": "ff120f3e-076b-4e71-b382-bf6f76df3a77", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[10.0, 54.31], [10.0, 54.31], [10.0, 54.31], [10.0, 54.31], [10.0, 54.31]]]}, "properties": {"themes": [{"concepts": [{"id": "farming"}], "scheme": "https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_TopicCategoryCode"}, {"concepts": [{"id": "Soil"}, {"id": "cover plants"}, {"id": "proteomics"}, {"id": "functional genomics"}, {"id": "phylum"}, {"id": "Zea mays"}], "scheme": "AGROVOC Multilingual agricultural thesaurus"}, {"concepts": [{"id": "opendata"}, {"id": "Trifolium pretense"}, {"id": "Trifolium repens"}, {"id": "Brassica napus"}, {"id": "Raphanus sativus var. oleiformis"}, {"id": "Festuca arundinacea"}, {"id": "Lolium perenne"}], "scheme": "Individual"}, {"concepts": [{"id": "Boden"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}], "rights": "Restrictions applied to assure the protection of privacy or intellectual property, and any special restrictions or limitations or warnings on using the resource or metadata. Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of the Rhizo4Bio - RootWayS's research activities.\" Although every care has been taken in preparing and testing the data, the Rhizo4Bio - RootWayS and the BonaRes Data Centre cannot guarantee that the data are correct; neither does the Rhizo4Bio - RootWayS and the BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The Rhizo4Bio - RootWayS and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data.", "updated": "2023-08-03", "type": "Dataset", "created": "2023-07-12", "language": "eng", "title": "Taxonomic abundance of bacteria and different functional metabolic pathways in bulk soil and maize rhizosphere at Hohenschulen field site in the first trial year of Rhizo4Bio \u2013 RootWayS project. - Qualitative and quantitative estimation of soil bacteria", "description": "This file contains information the taxonomic details (qualitative and quantitative) for soil samples taken six weeks after cover crop termination (maize germination), eight weeks after cover crop termination (maize at BBCH stage 13) and 14 weeks after cover crop termination (maize at BBCH stage 33). The phyla of bacteria have been identified using Illumina MiSeq sequencing (16S, in the V3 region) and were quantified by real-time qPCR in terms of copy numbers per mg of soil used for DNA extraction.\n\nGeneral description see mother table: (https://doi.org/10.20387/bonares-1a58-yk35); Related datasets are listed in the metadata element 'Related Identifier'.\nDataset version 1.0", "formats": [{"name": "CSV"}], "keywords": ["Soil", "cover plants", "proteomics", "functional genomics", "phylum", "Zea mays", "opendata", "Trifolium pretense", "Trifolium repens", "Brassica napus", "Raphanus sativus var. oleiformis", "Festuca arundinacea", "Lolium perenne", "Boden"], "contacts": [{"name": "Debjyoti Ghosh", "organization": "Helmholtz-Zentrum f\u00fcr Umweltforschung, Leipzig", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "debjyoti.ghosh@ufz.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": null}]}, {"name": "Sandra Spielvogel", "organization": "Kiel University", "position": null, "roles": ["projectLeader"], "phones": [{"value": null}], "emails": [{"value": "s.spielvogel@soils.uni-kiel.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0003-2310-5975", "name_url": "", "description": "OCRID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": null, "organization": "Leibniz Centre for Agricultural Landscape Research (ZALF)", "position": "Research Platform 'Data Analysis & Simulation' - Workgroup Research Data Management", "roles": ["publisher"], "phones": [{"value": "+49 33432 82 300"}], "emails": [{"value": "dataservice@zalf.de"}], "addresses": [{"deliveryPoint": ["Eberswalder Strasse 84"], "city": "M\u00fcncheberg", "administrativeArea": "Brandenburg", "postalCode": "15374", "country": "Germany"}], "links": [{"href": null}]}, {"name": "Iris Zimmermann", "organization": "Kiel University", "position": null, "roles": ["projectManager"], "phones": [{"value": null}], "emails": [{"value": "i.zimmermann@soils.uni-kiel.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": null}]}, {"organization": "Helmholtz-Zentrum f\u00fcr Umweltforschung, Leipzig", "roles": ["contributor"]}], "title_alternate": "Data collection: Part 3/3, table: Qualitative and quantitative estimation of taxonomic abundance of bacteria in soil after different winter cover crop variations"}, "links": [{"href": "https://maps.bonares.de/mapapps/resources/apps/bonares/index.html?lang=en&mid=3c0aaeae-4b91-4339-a91c-def691615d75", "rel": "download"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3c0aaeae-4b91-4339-a91c-def691615d75", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "ff120f3e-076b-4e71-b382-bf6f76df3a77", "name": "item", "description": "ff120f3e-076b-4e71-b382-bf6f76df3a77", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ff120f3e-076b-4e71-b382-bf6f76df3a77"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-08-03T00:00:00Z"}}, {"id": "3add4bdd-a652-4a18-850a-d3bf96966e71", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[10.0, 54.31], [10.0, 54.31], [10.0, 54.31], [10.0, 54.31], [10.0, 54.31]]]}, "properties": {"themes": [{"concepts": [{"id": "farming"}], "scheme": "https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_TopicCategoryCode"}, {"concepts": [{"id": "Soil"}, {"id": "cover plants"}, {"id": "proteomics"}, {"id": "functional genomics"}, {"id": "phylum"}, {"id": "Zea mays"}], "scheme": "AGROVOC Multilingual agricultural thesaurus"}, {"concepts": [{"id": "opendata"}, {"id": "Trifolium pretense"}, {"id": "Trifolium repens"}, {"id": "Brassica napus"}, {"id": "Raphanus sativus var. oleiformis"}, {"id": "Festuca arundinacea"}, {"id": "Lolium perenne"}], "scheme": "Individual"}, {"concepts": [{"id": "Boden"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}], "rights": "Restrictions applied to assure the protection of privacy or intellectual property, and any special restrictions or limitations or warnings on using the resource or metadata. Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of the Rhizo4Bio - RootWayS's research activities.\" Although every care has been taken in preparing and testing the data, the Rhizo4Bio - RootWayS and the BonaRes Data Centre cannot guarantee that the data are correct; neither does the Rhizo4Bio - RootWayS and the BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The Rhizo4Bio - RootWayS and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data.", "updated": "2023-08-03", "type": "Dataset", "created": "2023-07-12", "language": "eng", "title": "Taxonomic abundance of bacteria and different functional metabolic pathways in bulk soil and maize rhizosphere at Hohenschulen field site in the first trial year of Rhizo4Bio \u2013 RootWayS project. - Estimation of the functional pathways by bacteria in soil", "description": "This file contains details about the different functional pathways taking place in the rhizosphere, detritus sphere, and bulk soil six weeks after cover crop termination (maize germination), eight weeks after cover crop termination (maize at BBCH stage 13) and 14 weeks after cover crop termination (maize at BBCH stage 33). This file also gives detailed quantification of the bacterial phyla contribution to each pathway in each type of sample, thereby giving a better overview of the dynamic metabolism and ideas to explain the observations seen about the biological processes. The pathways are the KEGG pathways, which are a collection of databases dealing with genomes, biological pathways, diseases, drugs, and chemical substances.\n\nGeneral description see mother table: (https://doi.org/10.20387/bonares-1a58-yk35); Related datasets are listed in the metadata element 'Related Identifier'.\nDataset version 1.0", "formats": [{"name": "CSV"}], "keywords": ["Soil", "cover plants", "proteomics", "functional genomics", "phylum", "Zea mays", "opendata", "Trifolium pretense", "Trifolium repens", "Brassica napus", "Raphanus sativus var. oleiformis", "Festuca arundinacea", "Lolium perenne", "Boden"], "contacts": [{"name": "Debjyoti Ghosh", "organization": "Helmholtz-Zentrum f\u00fcr Umweltforschung, Leipzig", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "debjyoti.ghosh@ufz.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": null}]}, {"name": "Sandra Spielvogel", "organization": "Kiel University", "position": null, "roles": ["projectLeader"], "phones": [{"value": null}], "emails": [{"value": "s.spielvogel@soils.uni-kiel.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0003-2310-5975", "name_url": "", "description": "OCRID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": null, "organization": "Leibniz Centre for Agricultural Landscape Research (ZALF)", "position": "Research Platform 'Data Analysis & Simulation' - Workgroup Research Data Management", "roles": ["publisher"], "phones": [{"value": "+49 33432 82 300"}], "emails": [{"value": "dataservice@zalf.de"}], "addresses": [{"deliveryPoint": ["Eberswalder Strasse 84"], "city": "M\u00fcncheberg", "administrativeArea": "Brandenburg", "postalCode": "15374", "country": "Germany"}], "links": [{"href": null}]}, {"name": "Iris Zimmermann", "organization": "Kiel University", "position": null, "roles": ["projectManager"], "phones": [{"value": null}], "emails": [{"value": "i.zimmermann@soils.uni-kiel.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": null}]}, {"organization": "Helmholtz-Zentrum f\u00fcr Umweltforschung, Leipzig", "roles": ["contributor"]}], "title_alternate": "Data collection: Part 2/3, table: Estimation of the functional pathways taking place in the root rhizosphere, detritus sphere, and bulk soil and contributed by bacteria in soil after different winter cover crop variations"}, "links": [{"href": "https://maps.bonares.de/mapapps/resources/apps/bonares/index.html?lang=en&mid=3c0aaeae-4b91-4339-a91c-def691615d75", "rel": "download"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3c0aaeae-4b91-4339-a91c-def691615d75", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3add4bdd-a652-4a18-850a-d3bf96966e71", "name": "item", "description": "3add4bdd-a652-4a18-850a-d3bf96966e71", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3add4bdd-a652-4a18-850a-d3bf96966e71"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-08-03T00:00:00Z"}}, {"id": "PMC11341717", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-19T16:23:33Z", "type": "Journal Article", "created": "2024-08-23", "title": "A NAC triad modulates plant immunity by negatively regulating N-hydroxy pipecolic acid biosynthesis", "description": "N-hydroxy\u00a0pipecolic acid (NHP) plays an important role in plant immunity. In contrast to its biosynthesis, our current knowledge with respect to the transcriptional regulation of the NHP pathway is limited. This study commences with the engineering of Arabidopsis plants that constitutively produce high NHP levels and display enhanced immunity. Label-free proteomics reveals a NAC-type transcription factor (NAC90) that is strongly induced in these plants. We find that NAC90 is a target gene of SAR DEFICIENT 1 (SARD1) and induced by pathogen, salicylic acid (SA), and NHP. NAC90 knockout mutants exhibit constitutive immune activation, earlier senescence, higher levels of NHP and SA, as well as increased expression of NHP and SA biosynthetic genes. In contrast, NAC90 overexpression lines are compromised in disease resistance and accumulated reduced levels of NHP and SA. NAC90 could interact with NAC61 and NAC36 which are also induced by pathogen, SA, and NHP. We next discover that this protein triad directly represses expression of the NHP and SA biosynthetic genes AGD2-LIKE DEFENSE RESPONSE PROTEIN 1 (ALD1), FLAVIN MONOOXYGENASE 1 (FMO1), and ISOCHORISMATE SYNTHASE 1 (ICS1). Constitutive immune response in nac90 is abolished once blocking NHP biosynthesis in the fmo1 background, signifying that NAC90 negative regulation of immunity is mediated via NHP biosynthesis. Our findings expand the currently documented NHP regulatory network suggesting a model that together with NHP glycosylation, NAC repressors take part in a 'gas-and-brake' transcriptional mechanism to control NHP production and the plant growth and defense trade-off.", "keywords": ["Proteomics", "0301 basic medicine", "0303 health sciences", "Arabidopsis Proteins", "Science", "Q", "Arabidopsis", "Plants", " Genetically Modified", "Article", "03 medical and health sciences", "Gene Expression Regulation", " Plant", "Pipecolic Acids", "Plant Immunity", "Salicylic Acid", "Transcription Factors", "Plant Diseases", "Disease Resistance"]}, "links": [{"href": "https://doi.org/PMC11341717"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11341717", "name": "item", "description": "PMC11341717", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11341717"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-22T00:00:00Z"}}, {"id": "PXD002768", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-09-19T16:23:40Z", "type": "Dataset", "title": "Chicken spermatozoa protein extract analyzed by Top-down HRMS", "description": "Currently, evaluation of quality semen is based on traditional in vitro sperm tests as measures of motility, viability and acrosomic reaction. However, the results are not always well correlated with real male fertility. In the present study, we evaluated Intact Cell MALDI-TOF Mass Spectrometry (ICM-MS) method as diagnostic molecular tool to substitute in vitro tests in order to phenotype semen with more robustness. For that, we investigated a large male population of known fertility that included two highly different genetic lines (meat and egg laying lines). Our optimized ICM-MS-based method through automation and the construction of fertility-predictive mathematical models was able to discriminate individuals on their reproductive capacity in the two lines studied. Furthermore, we showed a better diagnostic accuracy than traditional in vitro sperm quality tests and demonstrated that differential markers presenting a high discriminating power between fertile and subfertile sperm cells in sperm were implicated in fertility processes. To characterize m/z peaks observed in ICM-MS spectra, we performed high-throughput top-down protein identification of sperm cell extracts. Therefore, we identified more than 75% of the m/z peaks, revealing that these comprises mostly degradation products of testis-specific proteins implicated in the most important functional pathways in sperm cells such as energy metabolism and structure/movement.", "keywords": ["Proteomics"]}, "links": [{"href": "https://doi.org/PXD002768"}, {"rel": "self", "type": "application/geo+json", "title": "PXD002768", "name": "item", "description": "PXD002768", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PXD002768"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-04-11T00:00:00Z"}}, {"id": "3c0aaeae-4b91-4339-a91c-def691615d75", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[10.0, 54.31], [10.0, 54.31], [10.0, 54.31], [10.0, 54.31], [10.0, 54.31]]]}, "properties": {"themes": [{"concepts": [{"id": "farming"}], "scheme": "https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_TopicCategoryCode"}, {"concepts": [{"id": "Soil"}, {"id": "cover plants"}, {"id": "proteomics"}, {"id": "functional genomics"}, {"id": "phylum"}, {"id": "Zea mays"}], "scheme": "AGROVOC Multilingual agricultural thesaurus"}, {"concepts": [{"id": "opendata"}, {"id": "Trifolium pretense"}, {"id": "Trifolium repens"}, {"id": "Brassica napus"}, {"id": "Raphanus sativus var. oleiformis"}, {"id": "Festuca arundinacea"}, {"id": "Lolium perenne"}], "scheme": "Individual"}, {"concepts": [{"id": "Boden"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}], "rights": "Restrictions applied to assure the protection of privacy or intellectual property, and any special restrictions or limitations or warnings on using the resource or metadata. Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of the Rhizo4Bio - RootWayS's research activities.\" Although every care has been taken in preparing and testing the data, the Rhizo4Bio - RootWayS and the BonaRes Data Centre cannot guarantee that the data are correct; neither does the Rhizo4Bio - RootWayS and the BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The Rhizo4Bio - RootWayS and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data.", "updated": "2023-08-03", "type": "Dataset", "created": "2023-07-12", "language": "eng", "title": "Taxonomic abundance of bacteria and different functional metabolic pathways in bulk soil and maize rhizosphere at Hohenschulen field site in the first trial year of Rhizo4Bio \u2013 RootWayS project.", "description": "In the winter half-year 2020/2021, winter cover crops were grown in 10 trial variants at the Hohenschulen trial site. The soils at the trial site are mainly stagnic Luvisols derived from glacial till. The variants were arranged in a randomized block design with four replications. In spring 2021, maize was planted on the site, and the soil of all plots was sampled in different depths at different growth stages of the maize. Soil samples were taken selectively from cover crop root channels and maize rhizosphere and analyzed for taxonomic abundance of bacteria and different functional metabolic pathways in the soil microbiome. 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