{"type": "FeatureCollection", "features": [{"id": "10.1016/j.scitotenv.2022.157225", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:17:25Z", "type": "Journal Article", "created": "2022-07-07", "title": "Perennial alley cropping contributes to decrease soil CO2 and N2O emissions and increase soil carbon sequestration in a Mediterranean almond orchard", "description": "The implementation of alley cropping in orchards can be a sustainable strategy to increase farm productivity by crop
diversification and contribute to climate change mitigation. In this research, we evaluated the short-termeffect of alley
cropping with reduced tillage on soil CO2 and N2O emissions and soil total organic carbon (TOC) in an almond orchard
under Mediterranean rainfed conditions. We compared an almond monoculture with tillage in all plot surface (MC)
with almond crop with reduced tillage and growth of Capparis spinosa (D1) and almond crop with reduced tillage and
growth of Thymus hyemalis (D2). For two years, soil CO2 and N2O were measured, with soil sampling at the start and
end of the experimental period. Results showed that CO2 emission rates followed the soil temperature pattern, while
N2O emissions were not correlated with temperature nor moisture. Soil CO2 emissions were significantly higher in
MC(87mgm\u22122 h\u22121), with no significant differences between D1 and D2 (69mgm\u22122 h\u22121). Some peaks in CO2 effluxes
were observed after tillage operations during warm days. Soil N2Oemission rateswere not significantly different among
treatments. Cumulative CO2 and CO2 equivalent (CO2e) emissions were significantly highest in MC. When CO2e emissions
were expressed on a crop production basis, D2 showed the significantly lowest values (5080 g kg\u22121) compared to
D1 (50,419 g kg\u22121) and MC (87,836 g kg\u22121), owing to the high thyme yield, additional to the almond yield. No production
was obtained for C. spinosa, since at least two more years are required. TOC did not change with time in MCneither
D1, but it significantly increased inD2 from3.85 g kg\u22121 in 2019 to 4.62 g kg\u22121 in 2021. Thus, alley cropping can contribute
to increase the agroecosystem productivity and reduce CO2 emissions. However, it is necessary to grow", "keywords": ["2. Zero hunger", "Carbon Sequestration", "N2O emissions", "Nitrous Oxide", "Agriculture", "Thyme", "2511.08 Mec\u00e1nica de Suelos (Agricultura)", "Carbon Dioxide", "15. Life on land", "CO2 emissions", "Prunus dulcis", "12. Responsible consumption", "Edafolog\u00eda y Qu\u00edmica Agr\u00edcola", "Soil", "Intercropping", "13. Climate action", "5102.01 Agricultura", "Soil carbon sequestration", "Caper", "Fertilizers"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2022.157225"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2022.157225", "name": "item", "description": "10.1016/j.scitotenv.2022.157225", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2022.157225"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-01T00:00:00Z"}}, {"id": "10.1094/pdis-08-18-1333-re", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:08Z", "type": "Journal Article", "created": "2019-02-25", "title": "AtoxigenicAspergillus flavusIsolates Endemic to Almond, Fig, and Pistachio Orchards in California with Potential to Reduce Aflatoxin Contamination in these Crops", "description": "

In California, aflatoxin contamination of almond, fig, and pistachio has become a serious problem in recent years due to long periods of drought and probably other climatic changes. The atoxigenic biocontrol product Aspergillus flavus AF36 has been registered for use to limit aflatoxin contamination of pistachio since 2012 and for use in almond and fig since 2017. New biocontrol technologies employ multiple atoxigenic genotypes because those provide greater benefits than using a single genotype. Almond, fig, and pistachio industries would benefit from a multi-strain biocontrol technology for use in these three crops. Several A. flavus vegetative compatibility groups (VCGs) associated with almond, fig, and pistachio composed exclusively of atoxigenic isolates, including the VCG to which AF36 belongs to, YV36, were previously characterized in California. Here, we report additional VCGs associated with either two or all three crops. Representative isolates of 12 atoxigenic VCGs significantly (P < 0.001) reduced (>80%) aflatoxin accumulation in almond and pistachio when challenged with highly toxigenic isolates of A. flavus and A. parasiticus under laboratory conditions. Isolates of the evaluated VCGs, including AF36, constitute valuable endemic, well-adapted, and efficient germplasm to design a multi-crop, multi-strain biocontrol strategy for use in tree crops in California. Availability of such a strategy would favor long-term atoxigenic A. flavus communities across the affected areas of California, and this would result in securing domestic and export markets for the nut crop and fig farmer industries and, most importantly, health benefits to consumers.

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Climate action", "Pistacia", "pistachios", "Microbial Interactions", "figs", "california", "Aspergillus flavus"]}, "links": [{"href": "https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-08-18-1333-RE"}, {"href": "https://doi.org/10.1094/pdis-08-18-1333-re"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Disease", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1094/pdis-08-18-1333-re", "name": "item", "description": "10.1094/pdis-08-18-1333-re", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1094/pdis-08-18-1333-re"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0199127", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:17Z", "type": "Journal Article", "created": "2018-06-20", "title": "Fungal communities associated with almond throughout crop development: Implications for aflatoxin biocontrol management in California", "description": "Interactions between pathogenic and nonpathogenic fungal species in the tree canopy are complex and can determine if disease will manifest in the plant and in other organisms such as honey bees. Seasonal dynamics of fungi were studied in an almond orchard in California where experimental release of the atoxigenic biopesticide Aspergillus flavus AF36 to displace toxigenic Aspergillus strains has been conducted for five years. The presence of the vegetative compatibility group (VCG) YV36, to which AF36 belongs, in the blossoms, and the honey bees that attend these blossoms, was assessed. In blossoms, A. flavus frequencies ranged from 0 to 4.5%, depending on the year of study. Frequencies of honey bees carrying A. flavus ranged from 6.5 to 10%. Only one A. flavus isolate recovered from a blossom in 2016 belonged to YV36, while members of the VCG were not detected contaminating honey bees. Exposure of pollinator honey bees to AF36 was detected to be very low. The density of several Aspergillus species was found to increase during almond hull split and throughout the final stages of maturation; this also occurred in pistachio orchards during the maturation period. Additionally, we found that AF36 effectively limited almond aflatoxin contamination in laboratory assays. This study provides knowledge and understanding of the seasonal dynamics of Aspergillus fungi and will help design aflatoxin management strategies for almond. The evidence of the low levels of VCG YV36 encountered on almond blossoms and bees during pollination and AF36's effectiveness in limiting aflatoxin contamination in almond provided additional support for the registration of AF36 with USEPA to use in almond in California.", "keywords": ["honey bees", "Crop and Pasture Production", "Crops", " Agricultural", "0301 basic medicine", "aflatoxins", "570", "General Science & Technology", "Science", "Veterinary and Food Sciences", "Crops", "Food Contamination", "Flowers", "Microbiology", "630", "California", "Trees", "03 medical and health sciences", "aspergillus flavus", "Aflatoxins", "Species Specificity", "Animals", "Nuts", "california", "Pest Control", " Biological", "Pollination", "2. 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Seasonal dynamics of fungi were studied in an almond orchard in California where experimental release of the atoxigenic biopesticide Aspergillus flavus AF36 to displace toxigenic Aspergillus strains has been conducted for five years. The presence of the vegetative compatibility group (VCG) YV36, to which AF36 belongs, in the blossoms, and the honey bees that attend these blossoms, was assessed. In blossoms, A. flavus frequencies ranged from 0 to 4.5%, depending on the year of study. Frequencies of honey bees carrying A. flavus ranged from 6.5 to 10%. Only one A. flavus isolate recovered from a blossom in 2016 belonged to YV36, while members of the VCG were not detected contaminating honey bees. Exposure of pollinator honey bees to AF36 was detected to be very low. The density of several Aspergillus species was found to increase during almond hull split and throughout the final stages of maturation; this also occurred in pistachio orchards during the maturation period. Additionally, we found that AF36 effectively limited almond aflatoxin contamination in laboratory assays. This study provides knowledge and understanding of the seasonal dynamics of Aspergillus fungi and will help design aflatoxin management strategies for almond. The evidence of the low levels of VCG YV36 encountered on almond blossoms and bees during pollination and AF36's effectiveness in limiting aflatoxin contamination in almond provided additional support for the registration of AF36 with USEPA to use in almond in California.", "keywords": ["honey bees", "Crop and Pasture Production", "Crops", " Agricultural", "0301 basic medicine", "aflatoxins", "570", "General Science & Technology", "Science", "Veterinary and Food Sciences", "Crops", "Food Contamination", "Flowers", "Microbiology", "630", "California", "Trees", "03 medical and health sciences", "aspergillus flavus", "Aflatoxins", "Species Specificity", "Animals", "Nuts", "california", "Pest Control", " Biological", "Pollination", "2. 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In California, aflatoxin contamination of almond, fig, and pistachio has become a serious problem in recent years due to long periods of drought and probably other climatic changes. The atoxigenic biocontrol product Aspergillus flavus AF36 has been registered for use to limit aflatoxin contamination of pistachio since 2012 and for use in almond and fig since 2017. New biocontrol technologies employ multiple atoxigenic genotypes because those provide greater benefits than using a single genotype. Almond, fig, and pistachio industries would benefit from a multi-strain biocontrol technology for use in these three crops. Several A. flavus vegetative compatibility groups (VCGs) associated with almond, fig, and pistachio composed exclusively of atoxigenic isolates, including the VCG to which AF36 belongs to, YV36, were previously characterized in California. Here, we report additional VCGs associated with either two or all three crops. Representative isolates of 12 atoxigenic VCGs significantly (P < 0.001) reduced (>80%) aflatoxin accumulation in almond and pistachio when challenged with highly toxigenic isolates of A. flavus and A. parasiticus under laboratory conditions. Isolates of the evaluated VCGs, including AF36, constitute valuable endemic, well-adapted, and efficient germplasm to design a multi-crop, multi-strain biocontrol strategy for use in tree crops in California. Availability of such a strategy would favor long-term atoxigenic A. flavus communities across the affected areas of California, and this would result in securing domestic and export markets for the nut crop and fig farmer industries and, most importantly, health benefits to consumers.

", "keywords": ["2. Zero hunger", "0301 basic medicine", "aflatoxins", "0303 health sciences", "almonds", "Food Contamination", "15. Life on land", "Ficus", "Prunus dulcis", "6. Clean water", "California", "03 medical and health sciences", "aspergillus flavus", "Aflatoxins", "13. Climate action", "Pistacia", "pistachios", "Microbial Interactions", "figs", "california", "Aspergillus flavus"]}, "links": [{"href": "https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-08-18-1333-RE"}, {"href": "https://doi.org/10568/100306"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Disease", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10568/100306", "name": "item", "description": "10568/100306", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10568/100306"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Prunus+dulcis&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Prunus+dulcis&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Prunus+dulcis&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Prunus+dulcis&offset=5", "hreflang": "en-US"}], "numberMatched": 5, "numberReturned": 5, "distributedFeatures": [], "timeStamp": "2026-06-24T13:19:35.131019Z"}