{"type": "FeatureCollection", "features": [{"id": "10.1007/978-3-319-39782-5_27-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:32Z", "type": "Report", "created": "2019-01-24", "title": "Genetics and Ecology of Isoprene Degradation", "description": "Approximately 550 million tonnes of the monoterpene, isoprene, are emitted to the atmosphere annually, principally from terrestrial plants. In contrast to methane, which is emitted in similar quantities, little is known about the biodegradation of isoprene. However, 30 years ago, bacteria capable of living on isoprene as a sole source of carbon and energy were described, although they were not investigated in detail. Recently there has been renewed interest in the potential of bacteria living in soils, marine sediments, and on the leaves of plants to degrade isoprene. Isolates capable of isoprene metabolism use a multicomponent soluble monooxygenase, which contains a diiron center at the active site, to oxidize isoprene to the epoxide, and all isolates described to date depend on glutathione for subsequent metabolic steps. The diversity of isoprene degraders has been investigated in terrestrial and marine environments using DNA-stable isotope probing (DNA-SIP), together with the use of gene probes targeting the monooxygenase active-site subunit. Gaps in our knowledge and future research directions are described.", "keywords": ["570", "550", "13. Climate action", "QR Microbiology"], "contacts": [{"organization": "Crombie, Andrew T, Mejia-Florez, Nasmille L, McGenity, Terry J, Murrell, J Colin,", "roles": ["creator"]}]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/978-3-319-39782-5"}, {"href": "http://link.springer.com/content/pdf/10.1007/978-3-319-39782-5_27-1"}, {"href": "https://doi.org/10.1007/978-3-319-39782-5_27-1"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/978-3-319-39782-5_27-1", "name": "item", "description": "10.1007/978-3-319-39782-5_27-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/978-3-319-39782-5_27-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-18T00:00:00Z"}}, {"id": "10.1111/1462-2920.13842", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:38Z", "type": "Journal Article", "created": "2017-06-27", "title": "Identification and characterisation of isoprene-degrading bacteria in an estuarine environment", "description": "Summary<p>Approximately one\uffe2\uff80\uff90third of volatile organic compounds (VOCs) emitted to the atmosphere consists of isoprene, originating from the terrestrial and marine biosphere, with a profound effect on atmospheric chemistry. However, isoprene provides an abundant and largely unexplored source of carbon and energy for microbes. The potential for isoprene degradation in marine and estuarine samples from the Colne Estuary, UK, was investigated using DNA\uffe2\uff80\uff90Stable Isotope Probing (DNA\uffe2\uff80\uff90SIP). Analysis at two timepoints showed the development of communities dominated by Actinobacteria including members of the genera Mycobacterium, Rhodococcus, Microbacterium and Gordonia. Representative isolates, capable of growth on isoprene as sole carbon and energy source, were obtained from marine and estuarine locations, and isoprene\uffe2\uff80\uff90degrading strains of Gordonia and Mycobacterium were characterised physiologically and their genomes were sequenced. Genes predicted to be required for isoprene metabolism, including four\uffe2\uff80\uff90component isoprene monooxygenases (IsoMO), were identified and compared with previously characterised examples. Transcriptional and activity assays of strains growing on isoprene or alternative carbon sources showed that growth on isoprene is an inducible trait requiring a specific IsoMO. This study is the first to identify active isoprene degraders in estuarine and marine environments using DNA\uffe2\uff80\uff90SIP and to characterise marine isoprene\uffe2\uff80\uff90degrading bacteria at the physiological and molecular level.</p>", "keywords": ["0301 basic medicine", "570", "Volatile Organic Compounds", "0303 health sciences", "550", "Base Sequence", "610", "QR Microbiology", "Sequence Analysis", " DNA", "Environment", "6. Clean water", "Mixed Function Oxygenases", "Mycobacterium", "03 medical and health sciences", "Hemiterpenes", "13. Climate action", "Pentanes", "Butadienes", "Rhodococcus", "14. Life underwater", "Gordonia Bacterium", "Research Articles", "Genome", " Bacterial", "GE Environmental Sciences"]}, "links": [{"href": "https://ueaeprints.uea.ac.uk/id/eprint/63998/4/Published_manuscript.pdf"}, {"href": "http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13842/fullpdf"}, {"href": "https://doi.org/10.1111/1462-2920.13842"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.13842", "name": "item", "description": "10.1111/1462-2920.13842", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.13842"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-21T00:00:00Z"}}, {"id": "10.1016/j.ese.2020.100013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:16Z", "type": "Journal Article", "created": "2020-01-13", "title": "Microbial electrochemistry for bioremediation", "description": "Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment. Externally supplementation of electron donors or acceptors is often difficult to control and/or involves chemical additions with limited lifespan, residue formation or other adverse side effects. Microbial electrochemistry has evolved very fast in the past years - this field relates to the study of electrochemical interactions between microorganisms and solid-state electron donors or acceptors. Current can be supplied in such so-called bioelectrochemical systems (BESs) at low voltage to provide or extract electrons in a very precise manner. A plethora of metabolisms can be linked to electrical current now, from metals reductions to denitrification and dechlorination. In this perspective, we provide an overview of the emerging applications of BES and derived technologies towards the bioremediation field and outline how this approach can be game changing.", "keywords": ["0301 basic medicine", "AUTOTROPHIC DENITRIFICATION", "elecetrobioremediation", "Bioremediaci\u00f3", "FUEL-CELLS", "Environmental technology. Sanitary engineering", "Microbial biotechnology", "01 natural sciences", "POLYCYCLIC AROMATIC-HYDROCARBONS", "03 medical and health sciences", "WASTE-WATER", "DECHLORINATION", "TD Environmental technology. Sanitary engineering", "Electrochemistry", "POLLUTANTS", "GE1-350", "TD1-1066", "0105 earth and related environmental sciences", "NITRATE-CONTAMINATED GROUNDWATER", "ENVIRONMENTAL REMEDIATION", "Q Science (General)", "QR Microbiology", "NITROGEN REMOVAL", "6. Clean water", "Environmental sciences", "Electroqu\u00edmica", "ORGANIC", "BIOELECTROCHEMICAL SYSTEMS", "13. Climate action", "Earth and Environmental Sciences", "Perspective", "Biotecnologia microbiana", "Bioremediation"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/540323/1/1-s2.0-S2666498420300053-main.pdf"}, {"href": "https://doi.org/10.1016/j.ese.2020.100013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Ecotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ese.2020.100013", "name": "item", "description": "10.1016/j.ese.2020.100013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ese.2020.100013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "10.1186/s40168-018-0607-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:22Z", "type": "Journal Article", "created": "2018-12-07", "title": "Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment", "description": "Approximately 500\u00a0Tg of isoprene are emitted to the atmosphere annually, an amount similar to that of methane, and despite its significant effects on the climate, very little is known about the biological degradation of isoprene in the environment. Isolation and characterisation of isoprene degraders at the molecular level has allowed the development of probes targeting isoA encoding the \u03b1-subunit of the isoprene monooxygenase. This enzyme belongs to the soluble diiron centre monooxygenase family and catalyses the first step in the isoprene degradation pathway. The use of probes targeting key metabolic genes is a successful approach in molecular ecology to study specific groups of bacteria in complex environments. Here, we developed and tested a novel isoA PCR primer set to study the distribution, abundance, and diversity of isoprene degraders in a wide range of environments.The new isoA probes specifically amplified isoA genes from taxonomically diverse isoprene-degrading bacteria including members of the genera Rhodococcus, Variovorax, and Sphingopyxis. There was no cross-reactivity with genes encoding related oxygenases from non-isoprene degraders. Sequencing of isoA amplicons from DNA extracted from environmental samples enriched with isoprene revealed that most environments tested harboured a considerable variety of isoA sequences, with poplar leaf enrichments containing more phylogenetically diverse isoA genes. Quantification by qPCR using these isoA probes revealed that isoprene degraders are widespread in the phyllosphere, terrestrial, freshwater and marine environments. Specifically, soils in the vicinity of high isoprene-emitting trees contained the highest number of isoprene-degrading bacteria.This study provides the molecular ecology tools to broaden our knowledge of the distribution, abundance and diversity of isoprene degraders in the environment, which is a fundamental step necessary to assess the impact that microbes have in mitigating the effects of this important climate-active gas.", "keywords": ["0301 basic medicine", "570", "Isoprene", "Climate", "Mixed Function Oxygenases", "Microbial ecology", "Comamonadaceae", "03 medical and health sciences", "Hemiterpenes", "Bacterial Proteins", "Butadienes", "Isoprene monooxygenase", "Rhodococcus", "Gene probes", "14. Life underwater", "Phylogeny", "Soil Microbiology", "DNA Primers", "0303 health sciences", "Bacteria", "Research", "isoA", "QR100-130", "QR Microbiology", "Sequence Analysis", " DNA", "15. Life on land", "Sphingomonadaceae", "Biodegradation", " Environmental", "13. Climate action"]}, "links": [{"href": "https://ueaeprints.uea.ac.uk/id/eprint/69294/1/Published_manuscript.pdf"}, {"href": "http://link.springer.com/content/pdf/10.1186/s40168-018-0607-0.pdf"}, {"href": "https://repository.essex.ac.uk/23630/1/s40168-018-0607-0.pdf"}, {"href": "https://doi.org/10.1186/s40168-018-0607-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40168-018-0607-0", "name": "item", "description": "10.1186/s40168-018-0607-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40168-018-0607-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2016.01247", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2016-08-08", "title": "Soil Fungal:Bacterial Ratios Are Linked to Altered Carbon Cycling", "description": "Despite several lines of observational evidence, there is a lack of consensus on whether higher fungal:bacterial (F:B) ratios directly cause higher soil carbon (C) storage. We employed RNA sequencing, protein profiling and isotope tracer techniques to evaluate whether differing F:B ratios are associated with differences in C storage. A mesocosm (13)C labeled foliar litter decomposition experiment was performed in two soils that were similar in their physico-chemical properties but differed in microbial community structure, specifically their F:B ratio (determined by PLFA analyses, RNA sequencing and protein profiling; all three corroborating each other). Following litter addition, we observed a consistent increase in abundance of fungal phyla; and greater increases in the fungal dominated soil; implicating the role of fungi in litter decomposition. Litter derived (13)C in respired CO2 was consistently lower, and residual (13)C in bulk SOM was higher in high F:B soil demonstrating greater C storage potential in the F:B dominated soil. We conclude that in this soil system, the increased abundance of fungi in both soils and the altered C cycling patterns in the F:B dominated soils highlight the significant role of fungi in litter decomposition and indicate that F:B ratios are linked to higher C storage potential.", "keywords": ["Microbiology (medical)", "Proteomics", "0301 basic medicine", "environment/Bioclimatology", "Supplementary Data", "[SDE.MCG]Environmental Sciences/Global Changes", "stable isotopes", "litter decomposition", "Microbiology", "03 medical and health sciences", "proteomics", "[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "[SDV.EE]Life Sciences [q-bio]/Ecology", "[SDU.STU.GC] Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "soil carbon", "European Commission", "bacteria", "Stable isotopes", "2. Zero hunger", "655240", "0303 health sciences", "Bacteria", "Litter decomposition", "Fungi", "RNA sequencing", "QR Microbiology", "15. Life on land", "Soil carbon", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "QR1-502", "6. Clean water", "QR", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "[SDE.MCG] Environmental Sciences/Global Changes", "[SDV.EE] Life Sciences [q-bio]/Ecology", " environment", "[SDV.EE.BIO] Life Sciences [q-bio]/Ecology", " environment/Bioclimatology", "[SDV.EE.BIO]Life Sciences [q-bio]/Ecology", "fungi", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "environment"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2016.01247"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.01247", "name": "item", "description": "10.3389/fmicb.2016.01247", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.01247"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-08-09T00:00:00Z"}}, {"id": "2164/13228", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:25:42Z", "type": "Journal Article", "created": "2016-08-08", "title": "Soil Fungal:Bacterial Ratios Are Linked to Altered Carbon Cycling", "description": "Despite several lines of observational evidence, there is a lack of consensus on whether higher fungal:bacterial (F:B) ratios directly cause higher soil carbon (C) storage. We employed RNA sequencing, protein profiling and isotope tracer techniques to evaluate whether differing F:B ratios are associated with differences in C storage. A mesocosm (13)C labeled foliar litter decomposition experiment was performed in two soils that were similar in their physico-chemical properties but differed in microbial community structure, specifically their F:B ratio (determined by PLFA analyses, RNA sequencing and protein profiling; all three corroborating each other). Following litter addition, we observed a consistent increase in abundance of fungal phyla; and greater increases in the fungal dominated soil; implicating the role of fungi in litter decomposition. Litter derived (13)C in respired CO2 was consistently lower, and residual (13)C in bulk SOM was higher in high F:B soil demonstrating greater C storage potential in the F:B dominated soil. We conclude that in this soil system, the increased abundance of fungi in both soils and the altered C cycling patterns in the F:B dominated soils highlight the significant role of fungi in litter decomposition and indicate that F:B ratios are linked to higher C storage potential.", "keywords": ["Microbiology (medical)", "Proteomics", "0301 basic medicine", "environment/Bioclimatology", "Supplementary Data", "[SDE.MCG]Environmental Sciences/Global Changes", "stable isotopes", "litter decomposition", "Microbiology", "03 medical and health sciences", "proteomics", "[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "[SDV.EE]Life Sciences [q-bio]/Ecology", "[SDU.STU.GC] Sciences of the Universe [physics]/Earth Sciences/Geochemistry", "soil carbon", "European Commission", "bacteria", "Stable isotopes", "2. Zero hunger", "655240", "0303 health sciences", "Bacteria", "Litter decomposition", "Fungi", "RNA sequencing", "QR Microbiology", "15. Life on land", "Soil carbon", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "QR1-502", "6. Clean water", "QR", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "[SDE.MCG] Environmental Sciences/Global Changes", "[SDV.EE] Life Sciences [q-bio]/Ecology", " environment", "[SDV.EE.BIO] Life Sciences [q-bio]/Ecology", " environment/Bioclimatology", "[SDV.EE.BIO]Life Sciences [q-bio]/Ecology", "fungi", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "environment"]}, "links": [{"href": "https://doi.org/2164/13228"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2164/13228", "name": "item", "description": "2164/13228", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2164/13228"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-08-09T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR+Microbiology&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR+Microbiology&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR+Microbiology&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR+Microbiology&offset=6", "hreflang": "en-US"}], "numberMatched": 6, "numberReturned": 6, "distributedFeatures": [], "timeStamp": "2026-07-26T04:12:12.804938Z"}