{"type": "FeatureCollection", "features": [{"id": "10.3389/fmicb.2016.01446", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2016-09-14", "description": "Soil management is fundamental to all agricultural systems and fertilization practices have contributed substantially to the impressive increases in food production. Despite the pivotal role of soil microorganisms in agro-ecosystems, we still have a limited understanding of the complex response of the soil microbiota to organic and mineral fertilization in the very long-term. Here, we report the effects of different fertilization regimes (mineral, organic and combined mineral and organic fertilization), carried out for more than a century, on the structure and activity of the soil microbiome. Organic matter content, nutrient concentrations, and microbial biomass carbon were significantly increased by mineral, and even more strongly by organic fertilization. Pyrosequencing revealed significant differences between the structures of bacterial and fungal soil communities associated to each fertilization regime. Organic fertilization increased bacterial diversity, and stimulated microbial groups (Firmicutes, Proteobacteria, and Zygomycota) that are known to prefer nutrient-rich environments, and that are involved in the degradation of complex organic compounds. In contrast, soils not receiving manure harbored distinct microbial communities enriched in oligotrophic organisms adapted to nutrient-limited environments, as Acidobacteria. The fertilization regime also affected the relative abundances of plant beneficial and detrimental microbial taxa, which may influence productivity and stability of the agroecosystem. As expected, the activity of microbial exoenzymes involved in carbon, nitrogen, and phosphorous mineralization were enhanced by both types of fertilization. However, in contrast to comparable studies, the highest chitinase and phosphatase activities were observed in the solely mineral fertilized soil. Interestingly, these two enzymes showed also a particular high biomass-specific activities and a strong negative relation with soil pH. As many soil parameters are known to change slowly, the particularity of unchanged fertilization treatments since 1902 allows a profound assessment of linkages between management and abiotic as well as biotic soil parameters. Our study revealed that pH and TOC were the majors, while nitrogen and phosphorous pools were minors, drivers for structure and activity of the soil microbial community. Due to the long-term treatments studied, our findings likely represent permanent and stable, rather than transient, responses of soil microbial communities to fertilization.", "keywords": ["Soil nutrients", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "long-term fertilization", "microbial biomass", "15. Life on land", "microbial activity", "Microbiology", "QR1-502", "03 medical and health sciences", "13. Climate action", "soil microbial communities", "soil nutrients", "454 pyrosequencing"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2016.01446"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.01446", "name": "item", "description": "10.3389/fmicb.2016.01446", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.01446"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-14T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.02597", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2019-11-08", "title": "New Insights Into Cinnamoyl Esterase Activity of Oenococcus oeni.", "description": "Some strains of Oenococcus oeni possess cinnamoyl esterase activity that can be relevant in the malolactic stage of wine production liberating hydroxycinnamic acids that are precursors of volatile phenols responsible for sensory faults. The objective of this study was to better understand the basis of the differential activity between strains. After initial screening, five commercial strains of O. oeni were selected, three were found to exhibit cinnamoyl esterase activity (CE+) and two not (CE-). Although the use of functional annotation of genes revealed genotypic variations between the strains, no specific genes common only to the three CE+ strains could explain the different activities. Pasteurized wine was used as a natural source of tartrate esters in growth and metabolism experiments conducted in MRS medium, whilst commercial trans-caftaric acid was used as substrate for enzyme assays. Detoxification did not seem to be the main biological mechanism involved in the activity since unlike its phenolic cleavage products and their immediate metabolites (trans-caffeic acid and 4-ethylcatechol), trans-caftaric acid was not toxic toward O. oeni. In the case of the two CE+ strains OenosTM and CiNeTM, wine-exposed samples showed a more rapid degradation of trans-caftaric acid than the unexposed ones. The CE activity was present in all cell-free extracts of both wine-exposed and unexposed strains, except in the cell-free extracts of the CE- strain CH11TM. This activity may be constitutive rather than induced by exposure to tartrate esters. Trans-caftaric acid was totally cleaved to trans-caffeic acid by cell-free extracts of the three CE+ strains, whilst cell-free extracts of the CE- strain CH16TM showed significantly lower activity, although higher for the strains in experiments with no prior wine exposure. The EstB28 esterase gene, found in the genomes of the 5 strains, did not reveal any difference on the upstream regulation and transport functionality between the strains. This study highlights the complexity of the basis of this activity in wine related O. oeni population. Variable cinnamoyl esterases or/and membrane transport activities in the O. oeni strains analyzed and a possible implication of wine molecules could explain this phenomenon.", "keywords": ["0301 basic medicine", "0303 health sciences", "tartrate esters", "cinnamoyl esterase", "Tartrate esters", "Hydroxycinnamic acids", "Wine", "hydroxycinnamic acids", "[SDV.IDA] Life Sciences [q-bio]/Food engineering", "Microbiology", "QR1-502", "03 medical and health sciences", "Cinnamoyl esterase", "wine", "Oenococcus oeni"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.02597"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.02597", "name": "item", "description": "10.3389/fmicb.2019.02597", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.02597"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-08T00:00:00Z"}}, {"id": "10.3389/fmicb.2022.983823", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:49Z", "type": "Journal Article", "created": "2022-11-08", "title": "Long-term effects of early-life rumen microbiota modulation on dairy cow production performance and methane emissions", "description": "

Rumen microbiota modulation during the pre-weaning period has been suggested as means to affect animal performance later in life. In this follow-up study, we examined the post-weaning rumen microbiota development differences in monozygotic twin-heifers that were inoculated (T-group) or not inoculated (C-group) (n\uffe2\uff80\uff89=\uffe2\uff80\uff894 each) with fresh adult rumen liquid during their pre-weaning period. We also assessed the treatment effect on production parameters and methane emissions of cows during their 1st lactation period. The rumen microbiota was determined by the 16S rRNA gene, 18S rRNA gene, and ITS1 amplicon sequencing. Animal weight gain and rumen fermentation parameters were monitored from 2 to 12\uffe2\uff80\uff89months of age. The weight gain was not affected by treatment, but butyrate proportion was higher in T-group in month 3 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.04). Apart from archaea (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.084), the richness of bacteria (p\uffe2\uff80\uff89<\uffe2\uff80\uff890.0001) and ciliate protozoa increased until month 7 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.004) and anaerobic fungi until month 11 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.005). The microbiota structure, measured as Bray\uffe2\uff80\uff93Curtis distances, continued to develop until months 3, 6, 7, and 10, in archaea, ciliate protozoa, bacteria, and anaerobic fungi, respectively (for all: p\uffe2\uff80\uff89=\uffe2\uff80\uff890.001). Treatment or age \uffc3\uff97 treatment interaction had a significant (p\uffe2\uff80\uff89<\uffe2\uff80\uff890.05) effect on 18 bacterial, 2 archaeal, and 6 ciliate protozoan taxonomic groups, with differences occurring mostly before month 4 in bacteria, and month 3 in archaea and ciliate protozoa. Treatment stimulated earlier maturation of prokaryote community in T-group before month 4 and earlier maturation of ciliate protozoa at month 2 (Random Forest: 0.75\uffe2\uff80\uff89month for bacteria and 1.5\uffe2\uff80\uff89month for protozoa). No treatment effect on the maturity of anaerobic fungi was observed. The milk production and quality, feed efficiency, and methane emissions were monitored during cow\uffe2\uff80\uff99s 1st lactation. The T-group had lower variation in energy-corrected milk yield (p\uffe2\uff80\uff89<\uffe2\uff80\uff890.001), tended to differ in pattern of residual energy intake over time (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.069), and had numerically lower somatic cell count throughout their 1st lactation period (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.081), but no differences between the groups in methane emissions (g/d, g/kg DMI, or g/kg milk) were observed. Our results demonstrated that the orally administered microbial inoculant induced transient changes in early rumen microbiome maturation. In addition, the treatment may influence the later production performance, although the mechanisms that mediate these effects need to be further explored.

", "keywords": ["microbiome modulation", "0301 basic medicine", "570", "ta412", "microbiome establishment", "Heifer", "dairy cow", "Rumen function", "Animal science", " dairy science", "Microbiology", "630", "Microbiome modulation", "QR1-502", "rumen function", "Microbiome establishment", "03 medical and health sciences", "Dairy cow", "heifer"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2022.983823"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2022.983823", "name": "item", "description": "10.3389/fmicb.2022.983823", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2022.983823"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-08T00:00:00Z"}}, {"id": "10.5194/we-19-39-2019", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:21:55Z", "type": "Journal Article", "created": "2019-06-06", "title": "Unassisted establishment of biological soil crusts on dryland road slopes", "description": "

Abstract. Understanding patterns of habitat natural recovery after human-made disturbances is critical for the conservation of ecosystems under high environmental stress, such as drylands. In particular, the unassisted establishment of nonvascular plants such as biological soil crusts or biocrust communities (e.g., soil lichens, mosses and cyanobacteria) in newly formed habitats is not yet fully understood. However, the potential of biocrusts to improve soil structure and function at the early stages of succession and promote ecosystem recovery is enormous. In this study, we evaluated the capacity of lichen biocrusts to spontaneously establish and develop on road slopes in a Mediterranean shrubland. We also compared taxonomic and functional diversity of biocrusts between road slopes and natural habitats in the surroundings. Biocrust richness and cover, species composition, and functional structure were measured in 17 road slopes (nine roadcuts and eight embankments) along a 13\u2009km highway stretch. Topography, soil properties and vascular plant communities of road slopes were also characterized. We used Kruskal\u2013Wallis tests and applied redundancy analysis (RDA) to test the effect of environmental scenario (road slopes vs.\u00a0natural habitat) and other local factors on biocrust features. We found that biocrusts were common in road slopes after \u223c20\u00a0years of construction with no human assistance needed. However, species richness and cover were still lower than in natural remnants. Also, functional structure was quite similar between roadcuts (i.e., after soil excavation) and natural remnants, and topography and soil properties influenced species composition while environmental scenario type and vascular plant cover did not. These findings further support the idea of biocrusts as promising restoration tools in drylands and confirm the critical role of edaphic factors in biocrust establishment and development in land-use change scenarios.

", "keywords": ["0106 biological sciences", "QH301-705.5", "Physiology", "Science", "GC1-1581", "QH1-199.5", "Oceanography", "Microbiology", "01 natural sciences", "GF1-900", "QP1-981", "GE1-350", "Biology (General)", "QH540-549.5", "2. Zero hunger", "Ecology", "Q", "Botany", "General. Including nature conservation", " geographical distribution", "15. Life on land", "QR1-502", "Environmental sciences", "QL1-991", "Human ecology. Anthropogeography", "QK1-989", "QH1-278.5", "Natural history (General)", "Zoology"]}, "links": [{"href": "https://we.copernicus.org/articles/19/39/2019/we-19-39-2019.pdf"}, {"href": "https://doi.org/10.5194/we-19-39-2019"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Web%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/we-19-39-2019", "name": "item", "description": "10.5194/we-19-39-2019", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/we-19-39-2019"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-06T00:00:00Z"}}, {"id": "10.3389/fmicb.2016.00525", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:47Z", "type": "Journal Article", "created": "2016-04-20", "title": "Belowground Response To Drought In A Tropical Forest Soil. I. Changes In Microbial Functional Potential And Metabolism", "description": "Global climate models predict a future of increased severity of drought in many tropical forests. Soil microbes are central to the balance of these systems as sources or sinks of atmospheric carbon (C), yet how they respond metabolically to drought is not well-understood. We simulated drought in the typically aseasonal Luquillo Experimental Forest, Puerto Rico, by intercepting precipitation falling through the forest canopy. This approach reduced soil moisture by 13% and water potential by 0.14 MPa (from -0.2 to -0.34). Previous results from this experiment have demonstrated that the diversity and composition of these soil microbial communities are sensitive to even small changes in soil water. Here, we show prolonged drought significantly alters the functional potential of the community and provokes a clear osmotic stress response, including the production of compatible solutes that increase intracellular C demand. Subsequently, a microbial population emerges with a greater capacity for extracellular enzyme production targeting macromolecular carbon. Significantly, some of these drought-induced functional shifts in the soil microbiota are attenuated by prior exposure to a short-term drought suggesting that acclimation may occur despite a lack of longer-term drought history.", "keywords": ["tropical forests", "0301 basic medicine", "2. Zero hunger", "570", "0303 health sciences", "Ecology", "Environmental Science and Management", "osmolytes", "drought", "Biological Sciences", "Medical microbiology", "15. Life on land", "551", "microbial ecology", "Microbiology", "QR1-502", "6. Clean water", "03 medical and health sciences", "13. Climate action", "Soil Sciences", "functional gene microarray"]}, "links": [{"href": "https://escholarship.org/content/qt5ts293tg/qt5ts293tg.pdf"}, {"href": "https://doi.org/10.3389/fmicb.2016.00525"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.00525", "name": "item", "description": "10.3389/fmicb.2016.00525", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.00525"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-04-20T00:00:00Z"}}, {"id": "10.3389/fmicb.2022.813480", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:49Z", "type": "Journal Article", "created": "2022-03-01", "title": "Metatranscriptomic analyses unravel dynamic changes in the microbial and metabolic transcriptional profiles in artisanal Austrian hard-cheeses during ripening", "description": "

Vorarlberger Bergk\uffc3\uffa4se (VB) is an artisanal Austrian washed-rind hard cheese produced from alpine cows\uffe2\uff80\uff99 raw milk without the addition of ripening cultures. Ripening time is a key factor in VB, as it strongly influences the microbial communities present in the cheeses and the organoleptic properties of the product. In this study, the microbial and metabolic transcriptional profiles in VB rinds at different ripening times were investigated. VB products before (30\uffe2\uff80\uff89days of ripening) and after (90\uffe2\uff80\uff89days of ripening) selling were selected, RNA was extracted and subjected to shotgun metatranscriptomic sequencing. The analysis revealed some of the previously described abundant bacterial taxa of Brevibacterium, Corynebacterium, Halomonas, Psychrobacter, and Staphylococcus to be highly active in VB rinds. Additionally, the investigation of most important metabolic pathways in cheese ripening clearly showed differences in the gene transcription profiles and the active microbiota between the two ripening points investigated. At 30\uffe2\uff80\uff89days of ripening, metabolic events related with the degradation of residual lactose, lactate, citrate, proteolysis, and lipolysis were significantly more transcribed and mainly associated with Staphylococcus. On the other hand, genes involved in the degradation of smaller compounds derived from previous metabolism (i.e., metabolism of free amino acids and fatty acids) were significantly more expressed in VB rinds with 90 of ripening, and mainly associated with Brevibacterium and Corynebacterium. These latter metabolic activities are responsible of the generation of compounds, such as methanethiol and 2,3-butanediol, that are very important for the flavor and aroma characteristics of cheeses. This study shows the dynamic changes in the gene transcriptional profiles associated with energy substrates metabolism and the generation of organoleptic compounds during VB ripening and uncovers bacterial taxa as key drivers of the ripening process. These taxa might be the target for future studies toward an accelerated cheese ripening and the enhancement of its organoleptic properties.

", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "791", "metatranscriptomics", "organoleptic compounds", "cheese ripening", "DegreeDisciplines::Life Sciences::Animal Sciences", "microbial dynamics", "Microbiology", "QR1-502", "differential gene transcription", "03 medical and health sciences", "metabolic pathways", "Bacteria; Identification; Quality; Communities; Microflora; Alignment; Pathways; Products; Genes; Acid", "DegreeDisciplines::Life Sciences::Food Science::Food Microbiology"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2022.813480"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2022.813480", "name": "item", "description": "10.3389/fmicb.2022.813480", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2022.813480"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-01T00:00:00Z"}}, {"id": "10.1051/bioconf/202516703010", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:18:02Z", "type": "Journal Article", "created": "2025-03-19", "title": "Simulation of Land Cover Change Impacts on Discharge and Sedimentation in Merawu Catchment, Indonesia", "description": "

Merawu Watershed is the upstream part of Serayu Watershed whose outlet is in Mrica Reservoir, Banjarnegara Regency. The capacity of Mrica reservoir continues to experience an increase in sedimentation from year to year, one of the main causes is the high erosion in Merawu Watershed. This study aims to determine the effect of changes in land cover on discharge and sedimentation in Merawu Watershed. Land cover changes were analyzed historically from maps from 2006 and 2022. The Merawu watershed hydrological model was built using the SWAT model and calibrated using the SUFI-2 algorithm. The effect of land cover changes was calculated by comparing the simulation results with land cover data from 2006 and 2022. The analysis of land cover changes shows that the Merawu Watershed experienced a decrease in secondary forest and cultivated land. The built SWAT model produced an R2 value of 0.6 and an NSE of 0.55 for calibration and showed an R2 value of 0.71 and an NSE of 0.69 during validation. From the simulation results the changes in land cover that occurred had an impact on an increase in the average daily discharge and sedimentation of 294.82% and 662.47%.Forest fires alter soil microbial communities that are essential to support ecosystem recovery following land burning. These alterations have different responses according to soil abiotic pre- and post-fire conditions and fire severity, among others, and tend to decrease along vegetation recovery over time. Thus, understanding the effects of fires on microbial soil communities is critical to evaluate ecosystem resilience and restoration strategies in fire-prone ecosystems. We studied the state of community-level physiological profiles (CLPPs) and the prokaryotic community structure of rhizosphere and bulk soils from two fire-affected sclerophyll forests (one surveyed 17 months and the other 33 months after fire occurrence) in the Mediterranean climate zone of central Chile. Increases in catabolic activity (by average well color development of CLPPs), especially in the rhizosphere as compared with the bulk soil, were observed in the most recently affected site only. Legacy of land burning was still clearly shaping soil prokaryote community structure, as shown by quantitative PCR (qPCR) and Illumina MiSeq sequencing of the V4 region of the 16S rRNA gene, particularly in the most recent fire-affected site. The qPCR copy numbers and alpha diversity indexes (Shannon and Pielou\uffe2\uff80\uff99s evenness) of sequencing data decreased in burned soils at both locations. Beta diversity analyses showed dissimilarity of prokaryote communities at both study sites according to fire occurrence, and NO3\uffe2\uff80\uff93 was the common variable explaining community changes for both of them. Acidobacteria and Rokubacteria phyla significantly decreased in burned soils at both locations, while Firmicutes and Actinobacteria increased. These findings provide a better understanding of the resilience of soil prokaryote communities and their physiological conditions in Mediterranean forests of central Chile following different time periods after fire, conditions that likely influence the ecological processes taking place during recovery of fire-affected ecosystems.

The symbiosis between Mesorhizobium japonicum R7A and Lotus japonicus Gifu is an important model system for investigating the role of bacterial exopolysaccharides (EPS) in plant-microbe interactions. Previously, we showed that R7A exoB mutants that are affected at an early stage of EPS synthesis and in lipopolysaccharide (LPS) synthesis induce effective nodules on L. japonicus Gifu after a delay, whereas exoU mutants affected in the biosynthesis of the EPS side chain induce small uninfected nodule primordia and are impaired in infection. The presence of a halo around the exoU mutant when grown on Calcofluor-containing media suggested the mutant secreted a truncated version of R7A EPS. A nonpolar \u0394 exoA mutant defective in the addition of the first glucose residue to the EPS backbone was also severely impaired symbiotically. Here, we used a suppressor screen to show that the severe symbiotic phenotype of the exoU mutant was due to the secretion of an acetylated pentasaccharide, as both monomers and oligomers, by the same Wzx/Wzy system that transports wild-type exopolysaccharide. We also present evidence that the \u0394 exoA mutant secretes an oligosaccharide by the same transport system, contributing to its symbiotic phenotype. In contrast, \u0394 exoYF and polar exoA and exoL mutants have a similar phenotype to exoB mutants, forming effective nodules after a delay. These studies provide substantial evidence that secreted incompatible EPS is perceived by the plant, leading to abrogation of the infection process.

[Formula: see text] Copyright \u00a9 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

", "keywords": ["0301 basic medicine", "0303 health sciences", "exo mutants", "exopolysaccharide secretion", "Polysaccharides", " Bacterial", "Botany", "Mesorhizobium", "Microbiology", "QR1-502", "03 medical and health sciences", "Bacterial Proteins", "QK1-989", "Mutation", "Mesorhizobium-Lotus symbiosis", "Lotus", "truncated exopolysaccharide", "Symbiosis", "Root Nodules", " Plant"]}, "links": [{"href": "https://doi.org/10.1094/mpmi-03-24-0024-r"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Molecular%20Plant-Microbe%20Interactions%C2%AE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1094/mpmi-03-24-0024-r", "name": "item", "description": "10.1094/mpmi-03-24-0024-r", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1094/mpmi-03-24-0024-r"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-09-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2022.859063", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:49Z", "type": "Journal Article", "created": "2022-05-17", "title": "Interacting Bioenergetic and Stoichiometric Controls on Microbial Growth", "description": "

Microorganisms function as open systems that exchange matter and energy with their surrounding environment. Even though mass (carbon and nutrients) and energy exchanges are tightly linked, there is a lack of integrated approaches that combine these fluxes and explore how they jointly impact microbial growth. Such links are essential to predicting how the growth rate of microorganisms varies, especially when the stoichiometry of carbon- (C) and nitrogen (N)-uptake is not balanced. Here, we present a theoretical framework to quantify the microbial growth rate for conditions of C-, N-, and energy-(co-) limitations. We use this framework to show how the C:N ratio and the degree of reduction of the organic matter (OM), which is also the electron donor, availability of electron acceptors (EAs), and the different sources of N together control the microbial growth rate under C, nutrient, and energy-limited conditions. We show that the growth rate peaks at intermediate values of the degree of reduction of OM under oxic and C-limited conditions, but not under N-limited conditions. Under oxic conditions and with N-poor OM, the growth rate is higher when the inorganic N (NInorg)-source is ammonium compared to nitrate due to the additional energetic cost involved in nitrate reduction. Under anoxic conditions, when nitrate is both EA and NInorg-source, the growth rates of denitrifiers and microbes performing the dissimilatory nitrate reduction to ammonia (DNRA) are determined by both OM degree of reduction and nitrate-availability. Consistent with the data, DNRA is predicted to foster growth under extreme nitrate-limitation and with a reduced OM, whereas denitrifiers are favored as nitrate becomes more available and in the presence of oxidized OM. Furthermore, the growth rate is reduced when catabolism is coupled to low energy yielding EAs (e.g., sulfate) because of the low carbon use efficiency (CUE). However, the low CUE also decreases the nutrient demand for growth, thereby reducing N-limitation. We conclude that bioenergetics provides a useful conceptual framework for explaining growth rates under different metabolisms and multiple resource-limitations.Environmental factors relating to soil pH are widely known to be important in structuring soil bacterial communities, yet the relationship between taxonomic community composition and functional diversity remains to be determined. Here, we analyze geographically distributed soils spanning a wide pH gradient and assess the functional gene capacity within those communities using whole genome metagenomics. Low pH soils consistently had fewer taxa (lower alpha and gamma diversity), but only marginal reductions in functional alpha diversity and equivalent functional gamma diversity. However, coherent changes in the relative abundances of annotated genes between pH classes were identified; with functional profiles clustering according to pH independent of geography. Differences in gene abundances were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-mediated mechanisms for organic C and N substrate acquisition. These findings show that bacterial functional versatility may not be constrained by taxonomy, and we further identify the range of physiological adaptations required to exist in soils of varying nutrient availability and edaphic conditions.Conventional methods of agricultural pest control and crop fertilisation are contributing to a crisis of biodiversity loss, biogeochemical cycle dysregulation, and ecosystem collapse. Thus, we must find ecologically responsible means to control disease and promote crop yields. The root-associated microbiome may contribute to this goal as microbes can aid plants with disease suppression, abiotic stress relief, and nutrient bioavailability. We applied 16S rRNA gene & fungal 18S rRNA gene (ITS2 region) amplicon sequencing to profile the diversity of the bacterial, archaeal & fungal communities associated with the roots of UK elite spring bread wheat varietyTriticum aestivum var.Paragon in different soils and developmental stages. This revealed that community composition shifted significantly for all three groups across compartments. This shift was most pronounced for bacteria and fungi, while we observed weaker selection on the ammonia oxidising archaea-dominated archaeal community. Across multiple soil types we found that soil inoculum was a significant driver of endosphere community composition, however several bacterial families were identified as core enriched taxa in all soil conditions. The most abundant of these wereStreptomycetaceaeandBurkholderiaceae.Moreover, as the plants senesce, both families were reduced in abundance, indicating that input from the living plant was required to maintain their abundance in the endosphere. To understand which microbes are using wheat root exudates in the rhizosphere, root exudates were labelled in a13CO2DNA stable isotope probing experiment. This shows that bacterial taxa within theBurkholderiaceaefamily among other core enriched taxa, such asPseudomonadaceae,were able to use root exudates butStreptomycetaceaewere not. Overall, this work provides a better understanding of the wheat microbiome, including the endosphere community. Understanding crop microbiome formation will contribute to ecologically responsible methods for yield improvement and biocontrol in the future.Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage two-phase biogas reactor systems at low vs. increased organic loading rates (OLRs) under mesophilic (37\uffe2\uff80\uff89\uffc2\uffb0C) and thermophilic (55\uffe2\uff80\uff89\uffc2\uffb0C) temperatures. To follow the adaptive response of the biomass-attached cellulolytic/hydrolytic biofilms at increasing ammonium/ammonia contents, genome-centered metagenomics and transcriptional profiling based on metagenome assembled genomes (MAGs) were conducted.

Results

In total, 78 bacterial and archaeal MAGs representing the most abundant members of the communities, and featuring defined quality criteria were selected and characterized in detail. Determination of MAG abundances under the tested conditions by mapping of the obtained metagenome sequence reads to the MAGs revealed that MAG abundance profiles were mainly shaped by the temperature but also by the OLR. However, the OLR effect was more pronounced for the mesophilic systems as compared to the thermophilic ones. In contrast, metatranscriptome mapping to MAGs subsequently normalized to MAG abundances showed that under thermophilic conditions, MAGs respond to increased OLRs by shifting their transcriptional activities mainly without adjusting their proliferation rates. This is a clear difference compared to the behavior of the microbiome under mesophilic conditions. Here, the response to increased OLRs involved adjusting of proliferation rates and corresponding transcriptional activities. The analysis led to the identification of MAGs positively responding to increased OLRs. The most outstanding MAGs in this regard, obviously well adapted to higher OLRs and/or associated conditions, were assigned to the order Clostridiales (Acetivibrio sp.) for the mesophilic biofilm and the orders Bacteroidales (Prevotella sp. and an unknown species), Lachnospirales (Herbinix sp. and Kineothrix sp.) and Clostridiales (Clostridium sp.) for the thermophilic biofilm. Genome-based metabolic reconstruction and transcriptional profiling revealed that positively responding MAGs mainly are involved in hydrolysis of grass silage, acidogenesis and / or\uffc2\uffa0acetogenesis.

Conclusions

An integrated -omics approach enabled the identification of new AD biofilm keystone species featuring outstanding performance under stress conditions such as increased OLRs. Genome-based knowledge on the metabolic potential and transcriptional activity of responsive microbiome members will contribute to the development of improved microbiological AD management strategies for biomethanation of renewable biomass.

", "keywords": ["Integrated -omics", "Bioconversion", "0301 basic medicine", "570", "Polyomics", "Integrated-omics", "Biogas", "Microbiology", "7. Clean energy", "03 medical and health sciences", "Anaerobic digestion", "GE1-350", "2. Zero hunger", "0303 health sciences", "Metagenome assembled genomes", "Microbial community structure", "15. Life on land", "QR1-502", "6. Clean water", "3. Good health", "Environmental sciences", "Metagenome assembled genomes", " Integrated -omics", " Polyomics", " Anaerobic digestion", " Biogas", " Bioconversion", " Microbial community structure", " Methane", " Metabolic activity", "13. Climate action", "Metabolic activity", "Methane", "Research Article"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1186/s40793-020-00354-x.pdf"}, {"href": "https://doi.org/10.1186/s40793-020-00354-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-020-00354-x", "name": "item", "description": "10.1186/s40793-020-00354-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-020-00354-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-02T00:00:00Z"}}, {"id": "10.1186/s40793-020-00364-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:23Z", "type": "Journal Article", "created": "2020-05-01", "title": "Trichomes form genotype-specific microbial hotspots in the phyllosphere of tomato", "description": "Abstract

Background The plant phyllosphere is a well-studied habitat characterized by low nutrient availability and high community dynamics. In contrast, plant trichomes, known for their production of a large number of metabolites, are a yet unexplored habitat for microbes. We analyzed the phyllosphere as well as trichomes of two tomato genotypes (Solanum lycopersicum LA4024, S. habrochaites LA1777) by targeting bacterial 16S rRNA gene fragments.Results Leaves, leaves without trichomes, and trichomes alone harbored similar abundances of bacteria (108- 109 16S rRNA gene copy numbers per gram of sample). In contrast, bacterial diversity was found significantly increased in trichome samples (Shannon index: 4.4 vs. 2.5). Moreover, the community composition was significantly different when assessed with beta diversity analysis and corresponding statistical tests. At the bacterial class level, Alphaproteobacteria (23.6%) were significantly increased, whereas Bacilli (8.6%) were decreased in trichomes. The bacterial family Sphingomonadacea (8.4%) was identified as the most prominent, trichome-specific taxa; Burkholderiaceae and Actinobacteria showed similar pattern. Moreover, Sphingomonas was identified as a central element in the core microbiome of trichome samples, while distinct low-abundant bacterial families including Hymenobacteraceae and Alicyclobacillaceae were exclusively found in trichome samples. Niche preferences were statistically significant for both genotypes and genotype-specific enrichments were further observed.Conclusion Our results provide first evidence of a highly specific trichome microbiome in tomato and show the importance of micro-niches for the structure of bacterial communities on leaves. These findings provide further clues for breeding, plant pathology and protection as well as so far unexplored natural pathogen defense strategies.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "Plant microhabitat", "0303 health sciences", "Plant microbiome", "Trichomes", "15. Life on land", "Microbiology", "Bacterial communities", "QR1-502", "Environmental sciences", "03 medical and health sciences", "Solanum lycopersicum", "Solanum habrochaites", "plant microbiome", " bacterial communities", " plant-microbe interactions", " Solanum habrochaites", " Solanum lycopersicum", " plant microhabitat", " phyllosphere", " trichomes", "GE1-350", "Phyllosphere", "Plant-microbe interactions", "Research Article"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1186/s40793-020-00364-9.pdf"}, {"href": "https://doi.org/10.1186/s40793-020-00364-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-020-00364-9", "name": "item", "description": "10.1186/s40793-020-00364-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-020-00364-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-01T00:00:00Z"}}, {"id": "10.1186/s40793-021-00381-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:23Z", "type": "Journal Article", "created": "2021-02-10", "title": "Soil, senescence and exudate utilisation: characterisation of the\u00a0Paragon var. spring bread wheat root microbiome", "description": "Abstract

Conventional methods of agricultural pest control and crop fertilisation are contributing to a crisis of biodiversity loss, biogeochemical cycle dysregulation, and ecosystem collapse. Thus, we must find ecologically responsible means to control disease and promote crop yields. The root-associated microbiome may contribute to this goal as microbes can aid plants with disease suppression, abiotic stress relief, and nutrient bioavailability. We applied 16S rRNA gene & fungal 18S rRNA gene (ITS2 region) amplicon sequencing to profile the diversity of the bacterial, archaeal & fungal communities associated with the roots of UK elite spring bread wheat varietyTriticum aestivum var.Paragon in different soils and developmental stages. This revealed that community composition shifted significantly for all three groups across compartments. This shift was most pronounced for bacteria and fungi, while we observed weaker selection on the ammonia oxidising archaea-dominated archaeal community. Across multiple soil types we found that soil inoculum was a significant driver of endosphere community composition, however several bacterial families were identified as core enriched taxa in all soil conditions. The most abundant of these wereStreptomycetaceaeandBurkholderiaceae.Moreover, as the plants senesce, both families were reduced in abundance, indicating that input from the living plant was required to maintain their abundance in the endosphere. To understand which microbes are using wheat root exudates in the rhizosphere, root exudates were labelled in a13CO2DNA stable isotope probing experiment. This shows that bacterial taxa within theBurkholderiaceaefamily among other core enriched taxa, such asPseudomonadaceae,were able to use root exudates butStreptomycetaceaewere not. Overall, this work provides a better understanding of the wheat microbiome, including the endosphere community. Understanding crop microbiome formation will contribute to ecologically responsible methods for yield improvement and biocontrol in the future.

", "keywords": ["2. Zero hunger", "0301 basic medicine", "570", "0303 health sciences", "Exudate", "15. Life on land", "Senescence", "Microbiology", "630", "QR1-502", "Environmental sciences", "03 medical and health sciences", "Root", "Wheat", "GE1-350", "Microbiome", "Endosphere", "Research Article"]}, "links": [{"href": "http://oro.open.ac.uk/77831/1/40793_2021_Article_381.pdf"}, {"href": "https://ueaeprints.uea.ac.uk/id/eprint/80327/1/Published_Version.pdf"}, {"href": "https://link.springer.com/content/pdf/10.1186/s40793-021-00381-2.pdf"}, {"href": "https://doi.org/10.1186/s40793-021-00381-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-021-00381-2", "name": "item", "description": "10.1186/s40793-021-00381-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-021-00381-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-09T00:00:00Z"}}, {"id": "10.1186/s40793-021-00386-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:23Z", "type": "Journal Article", "created": "2021-08-26", "title": "Isoprene-degrading bacteria associated with the phyllosphere of Salix fragilis, a high isoprene-emitting willow of the northern hemisphere", "description": "AbstractBackground

Isoprene accounts for about half of total biogenic volatile organic compound emissions globally, and as a climate active gas it plays a significant and varied role in atmospheric chemistry. Terrestrial plants are the largest source of isoprene, with willow (Salix) making up one of the most active groups of isoprene producing trees. Bacteria act as a biological sink for isoprene and those bacteria associated with high isoprene-emitting trees may provide further insight into its biodegradation.

Results

A DNA-SIP experiment incubating willow (Salix fragilis) leaves with13C-labelled isoprene revealed an abundance ofComamonadaceae, Methylobacterium, MycobacteriumandPolaromonasin the isoprene degrading community when analysed by 16S rRNA gene amplicon sequencing. Metagenomic analysis of13C-enriched samples confirmed the abundance ofComamonadaceae, Acidovorax, Polaromonas, VariovoraxandRamlibacter. MycobacteriumandMethylobacteriumwere also identified after metagenomic analysis and aMycobacteriummetagenome-assembled genome (MAG) was recovered. This contained two complete isoprene degradation metabolic gene clusters, along with a propane monooxygenase gene cluster. Analysis of the abundance of the alpha subunit of the isoprene monooxygenase,isoA,in unenriched DNA samples revealed that isoprene degraders associated with willow leaves are abundant, making up nearly 0.2% of the natural bacterial community.

Conclusions

Analysis of the isoprene degrading community associated with willow leaves using DNA-SIP and focused metagenomics techniques enabled recovery of the genome of an active isoprene-degradingMycobacteriumspecies and provided valuable insight into bacteria involved in degradation of isoprene on the leaves of a key species of isoprene-emitting tree in the northern hemisphere.

", "keywords": ["Salix fragilis", "2. Zero hunger", "0301 basic medicine", "570", "0303 health sciences", "isoprene monooxygenase", "Isoprene", "Climate", "isoA", "15. Life on land", "Microbiology", "630", "QR1-502", "6. Clean water", "Environmental sciences", "03 medical and health sciences", "Willow tree", "13. Climate action", "Isoprene monooxygenase", "GE1-350", "willow tree", "isoprene", "climate", "DNA stable isotope probing", "Research Article"]}, "links": [{"href": "https://ueaeprints.uea.ac.uk/id/eprint/81258/1/Published_Version.pdf"}, {"href": "https://link.springer.com/content/pdf/10.1186/s40793-021-00386-x.pdf"}, {"href": "https://doi.org/10.1186/s40793-021-00386-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-021-00386-x", "name": "item", "description": "10.1186/s40793-021-00386-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-021-00386-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-26T00:00:00Z"}}, {"id": "10.3389/fmicb.2024.1473666", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:49Z", "type": "Journal Article", "created": "2024-10-24", "title": "Culturomics- and metagenomics-based insights into the soil microbiome preservation and application for sustainable agriculture", "description": "

Soil health is crucial for global food production in the context of an ever-growing global population. Microbiomes, a combination of microorganisms and their activities, play a pivotal role by biodegrading contaminants, maintaining soil structure, controlling nutrients\uffe2\uff80\uff99 cycles, and regulating the plant responses to biotic and abiotic stresses. Microbiome-based solutions along the soil-plant continuum, and their scaling up from laboratory experiments to field applications, hold promise for enhancing agricultural sustainability by harnessing the power of microbial consortia. Synthetic microbial communities, i.e., selected microbial consortia, are designed to perform specific functions. In contrast, natural communities leverage indigenous microbial populations that are adapted to local soil conditions, promoting ecosystem resilience, and reducing reliance on external inputs. The identification of microbial indicators requires a holistic approach. It is fundamental for current understanding the soil health status and for providing a comprehensive assessment of sustainable land management practices and conservation efforts. Recent advancements in molecular technologies, such as high-throughput sequencing, revealed the incredible diversity of soil microbiomes. On one hand, metagenomic sequencing allows the characterization of the entire genetic composition of soil microbiomes, and the examination of their functional potential and ecological roles; on the other hand, culturomics-based approaches and metabolic fingerprinting offer complementary information by providing snapshots of microbial diversity and metabolic activities both in and ex-situ. Long-term storage and cryopreservation of mixed culture and whole microbiome are crucial to maintain the originality of the sample in microbiome biobanking and for the development and application of microbiome-based innovation. This review aims to elucidate the available approaches to characterize diversity, function, and resilience of soil microbial communities and to develop microbiome-based solutions that can pave the way for harnessing nature\uffe2\uff80\uff99s untapped resources to cultivate crops in healthy soils, to enhance plant resilience to abiotic and biotic stresses, and to shape thriving ecosystems unlocking the potential of soil microbiomes is key to sustainable agriculture. Improving management practices by incorporating beneficial microbial consortia, and promoting resilience to climate change by facilitating adaptive strategies with respect to environmental conditions are the global challenges of the future to address the issues of climate change, land degradation and food security.In hot deserts daily/seasonal fluctuations pose great challenges to the resident organisms. However, these extreme ecosystems host unique microenvironments, such as the rhizosheath\uffe2\uff80\uff93root system of desert speargrasses in which biological activities and interactions are facilitated by milder conditions and reduced fluctuations. Here, we examined the bacterial microbiota associated with this structure and its surrounding sand in the desert speargrass Stipagrostis pungens under the contrasting environmental conditions of summer and winter in the Sahara Desert.

Results

The belowground rhizosheath\uffe2\uff80\uff93root system has higher nutrient and humidity contents, and cooler temperatures than the surrounding sand. The plant responds to the harsh environmental conditions of the summer by increasing the abundance and diversity of extracellular polymeric substances (EPS) compared to the winter. On the contrary, the bacterial community associated with the rhizosheath\uffe2\uff80\uff93root system and its interactome remain stable and, unlike the bulk sand, are unaffected by the seasonal environmental variations. The rhizosheath\uffe2\uff80\uff93root system bacterial communities are consistently dominated by Actinobacteria and Alphaproteobacteria and form distinct bacteria communities from those of bulk sand in the two seasons. The microbiome-stabilization mediated by the plant host acts to consistently retain beneficial bacteria with multiple plant growth promoting functions, including those capable to produce EPS, which increase the sand water holding capacity ameliorating the rhizosheath micro-environment.

Conclusions

Our results reveal the capability of plants in desert ecosystems to stabilize their below ground microbial community under seasonal contrasting environmental conditions, minimizing the heterogeneity of the surrounding bulk sand and contributing to the overall holobiont resilience under poly-extreme conditions.

", "keywords": ["Desert; Desertification; Environmental fluctuation; Environmentally-independent microbiome; Extracellular polymeric substances (EPS); PGP microorganisms; Plant legacy; Plant-microbiome; Rhizosheath", "Plant legacy", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Environmentally-independent microbiome", "15. Life on land", "Rhizosheath", "Microbiology", "QR1-502", "Environmental fluctuation", "Environmental sciences", "Plant-microbiome", "03 medical and health sciences", "PGP microorganisms", "13. Climate action", "Desert; Desertification; Environmental fluctuation; Environmentally-independent microbiome; Extracellular polymeric substances (EPS); PGP microorganisms; Plant legacy; Plant-microbiome; Rhizosheath;", "Extracellular polymeric substances (EPS)", "GE1-350", "Desert", "Desertification", "Research Article"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/921619/2/Marasco%20et%20al.%202022_Rhizosheat%20bact%20comm_EnvMicrobiome.pdf"}, {"href": "https://iris.unive.it/bitstream/10278/5089931/1/doi.org%3a10.1186%3as40793-022-00407-3.pdf"}, {"href": "https://arpi.unipi.it/bitstream/11568/1159772/2/Marasco%20et%20al.%20-%202022%20-%20Rhizosheath%e2%80%93root%20system%20changes%20exopolysaccharide%20.pdf"}, {"href": "https://flore.unifi.it/bitstream/2158/1285602/1/Marasco%20et%20al%20Env%20Microbiome%202022.pdf"}, {"href": "https://doi.org/10.1186/s40793-022-00407-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-022-00407-3", "name": "item", "description": "10.1186/s40793-022-00407-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-022-00407-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-01T00:00:00Z"}}, {"id": "10.1186/s40793-023-00479-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:23Z", "type": "Journal Article", "created": "2023-03-30", "title": "Abundance, classification and genetic potential of Thaumarchaeota in metagenomes of European agricultural soils: a meta-analysis", "description": "Abstract Background

For a sustainable production of food, research on agricultural soil microbial communities is inevitable. Due to its immense complexity, soil is still some kind of black box. Soil study designs for identifying microbiome members of relevance have various scopes and focus on particular environmental factors. To identify common features of soil microbiomes, data from multiple studies should be compiled and processed. Taxonomic compositions and functional capabilities of microbial communities associated with soils and plants have been identified and characterized in the past few decades. From a fertile Loess\uffe2\uff80\uff93Chernozem-type soil located in Germany, metagenomically assembled genomes (MAGs) classified as members of the phylum Thaumarchaeota/Thermoproteota were obtained. These possibly represent keystone agricultural soil community members encoding functions of relevance for soil fertility and plant health. Their importance for the analyzed microbiomes is corroborated by the fact that they were predicted to contribute to the cycling of nitrogen, feature the genetic potential to fix carbon dioxide and possess genes with predicted functions in plant-growth-promotion (PGP). To expand the knowledge on soil community members belonging to the phylum Thaumarchaeota, we conducted a meta-analysis integrating primary studies on European agricultural soil microbiomes.

Results

Taxonomic classification of the selected soil metagenomes revealed the shared agricultural soil core microbiome of European soils from 19 locations. Metadata reporting was heterogeneous between the different studies. According to the available metadata, we separated the data into 68 treatments. The phylum Thaumarchaeota is part of the core microbiome and represents a major constituent of the archaeal subcommunities in all European agricultural soils. At a higher taxonomic resolution, 2074 genera constituted the core microbiome. We observed that viral genera strongly contribute to variation in taxonomic profiles. By binning of metagenomically assembled contigs, Thaumarchaeota MAGs could be recovered from several European soil metagenomes. Notably, many of them were classified as members of the family Nitrososphaeraceae, highlighting the importance of this family for agricultural soils. The specific Loess-Chernozem Thaumarchaeota MAGs were most abundant in their original soil, but also seem to be of importance in other agricultural soil microbial communities. Metabolic reconstruction of Switzerland_1_MAG_2 revealed its genetic potential i.a. regarding carbon dioxide (CO$$_2$$ 2 ) fixation, ammonia oxidation, exopolysaccharide production and a beneficial effect on plant growth. Similar genetic features were also present in other reconstructed MAGs. Three Nitrososphaeraceae MAGs are all most likely members of a so far unknown genus.

Conclusions

On a broad view, European agricultural soil microbiomes are similarly structured. Differences in community structure were observable, although analysis was complicated by heterogeneity in metadata recording. Our study highlights the need for standardized metadata reporting and the benefits of networking open data. Future soil sequencing studies should also consider high sequencing depths in order to enable reconstruction of genome bins. Intriguingly, the family Nitrososphaeraceae commonly seems to be of importance in agricultural microbiomes.

", "keywords": ["2. Zero hunger", "570", "Soil microbial diversity", "Metagenomically assembled genomes", "Research", "European soil", "Open metagenome data analysis", "15. Life on land", "Microbiology", "Thaumarchaeota", "QR1-502", "Environmental sciences", "Agricultural microbiome", "Soil health", "GE1-350"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1186/s40793-023-00479-9.pdf"}, {"href": "https://doi.org/10.1186/s40793-023-00479-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-023-00479-9", "name": "item", "description": "10.1186/s40793-023-00479-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-023-00479-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-03-30T00:00:00Z"}}, {"id": "10.1128/mBio.00799-17", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2017-03-19", "title": "Bacterial Physiological Adaptations to Contrasting Edaphic Conditions Identified Using Landscape Scale Metagenomics", "description": "Abstract

Environmental factors relating to soil pH are widely known to be important in structuring soil bacterial communities, yet the relationship between taxonomic community composition and functional diversity remains to be determined. Here, we analyze geographically distributed soils spanning a wide pH gradient and assess the functional gene capacity within those communities using whole genome metagenomics. Low pH soils consistently had fewer taxa (lower alpha and gamma diversity), but only marginal reductions in functional alpha diversity and equivalent functional gamma diversity. However, coherent changes in the relative abundances of annotated genes between pH classes were identified; with functional profiles clustering according to pH independent of geography. Differences in gene abundances were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-mediated mechanisms for organic C and N substrate acquisition. These findings show that bacterial functional versatility may not be constrained by taxonomy, and we further identify the range of physiological adaptations required to exist in soils of varying nutrient availability and edaphic conditions.

", "keywords": ["Q Science", "0301 basic medicine", "330", "Supplementary Data", "ecophysiology", "Ecophysiology", "NE/E006353/1", "Bacterial Physiological Phenomena", "Microbiology", "Soil", "03 medical and health sciences", "Virology", "European Commission", "Ecosystem", "Phylogeny", "Soil Microbiology", "2. Zero hunger", "655240", "metagenomics", "0303 health sciences", "Bacteria", "Natural Environment Research Council (NERC)", "Q", "NE/M017125/1", "Biodiversity", "Hydrogen-Ion Concentration", "15. Life on land", "Adaptation", " Physiological", "soil microbiology", "QR1-502", "United Kingdom", "3. Good health", "Soil microbiology", "Metagenomics", "Genome", " Bacterial", "Research Article"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/117887v1.full.pdf"}, {"href": "https://journals.asm.org/doi/pdf/10.1128/mBio.00799-17"}, {"href": "https://doi.org/10.1128/mBio.00799-17"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mBio", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/mBio.00799-17", "name": "item", "description": "10.1128/mBio.00799-17", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/mBio.00799-17"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-03-18T00:00:00Z"}}, {"id": "10.1128/mbio.00455-24", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2024-03-25", "title": "Priorities, opportunities, and challenges for integrating microorganisms into Earth system models for climate change prediction", "description": "ABSTRACT

Climate change jeopardizes human health, global biodiversity, and sustainability of the biosphere. To make reliable predictions about climate change, scientists use Earth system models (ESMs) that integrate physical, chemical, and biological processes occurring on land, the oceans, and the atmosphere. Although critical for catalyzing coupled biogeochemical processes, microorganisms have traditionally been left out of ESMs. Here, we generate a \uffe2\uff80\uff9ctop 10\uffe2\uff80\uff9d list of priorities, opportunities, and challenges for the explicit integration of microorganisms into ESMs. We discuss the need for coarse-graining microbial information into functionally relevant categories, as well as the capacity for microorganisms to rapidly evolve in response to climate-change drivers. Microbiologists are uniquely positioned to collect novel and valuable information necessary for next-generation ESMs, but this requires data harmonization and transdisciplinary collaboration to effectively guide adaptation strategies and mitigation policy.

", "keywords": ["Naturgeografi", "Earth", " Planet", "Climate Change", "Microbiology", "traits", "biogeochemistry", "Humans", "Ecosystem", "Biomedical and Clinical Sciences", "Bacteria", "biogeochemistry; modeling; traits; climate change", "modeling", "Opinion/Hypothesis", "Biodiversity", "Biological Sciences", "Medical microbiology", "Models", " Theoretical", "15. Life on land", "QR1-502", "6. Clean water", "Climate Science", "3. Good health", "Climate Action", "climate change", "Physical Geography", "Medical Microbiology", "13. Climate action", "Biochemistry and cell biology", "Biochemistry and Cell Biology", "Generic health relevance", "Klimatvetenskap"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/mbio.00455-24"}, {"href": "https://doi.org/10.1128/mbio.00455-24"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mBio", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/mbio.00455-24", "name": "item", "description": "10.1128/mbio.00455-24", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/mbio.00455-24"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-08T00:00:00Z"}}, {"id": "10.1128/msystems.00562-19", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2020-01-13", "title": "Transcriptomic Response of Nitrosomonas europaea Transitioned from Ammonia- to Oxygen-Limited Steady-State Growth", "description": "

Nitrification is a ubiquitous microbially mediated process in the environment and an essential process in engineered systems such as wastewater and drinking water treatment plants. However, nitrification also contributes to fertilizer loss from agricultural environments, increasing the eutrophication of downstream aquatic ecosystems, and produces the greenhouse gas nitrous oxide. As ammonia-oxidizing bacteria are the most dominant ammonia-oxidizing microbes in fertilized agricultural soils, understanding their responses to a variety of environmental conditions is essential for curbing the negative environmental effects of nitrification. Notably, oxygen limitation has been reported to significantly increase nitric oxide and nitrous oxide production during nitrification. Here, we investigate the physiology of the best-characterized ammonia-oxidizing bacterium, Nitrosomonas europaea , growing under oxygen-limited conditions. Our results reinforce the notion that each cultivar on each location recruits a unique microbial community and that these communities are modulated by the vegetative growth stage of the plant. Moreover, inoculation of a Bacillus amyloliquefaciens strain QST713-based product on potatoes also changed the abundance of specific taxonomic groups and the structure of local networks in those locations where the product caused an increase in the yield.

", "keywords": ["Crops", " Agricultural", "0301 basic medicine", "2. Zero hunger", "Biological Products", "0303 health sciences", "Bacteria", "Microbiota", "Fungi", "High-Throughput Nucleotide Sequencing", "Agriculture", "Agricultural Inoculants", "15. Life on land", "Microbiology", "QR1-502", "United States", "Soil", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "Rhizosphere", "Soil Microbiology", "Research Article", "Solanum tuberosum"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/mSphere.00130-21"}, {"href": "https://doi.org/10.1128/msphere.00130-21"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msphere.00130-21", "name": "item", "description": "10.1128/msphere.00130-21", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msphere.00130-21"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-25T00:00:00Z"}}, {"id": "10.1128/msystems.00226-20", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2020-04-06", "title": "Tracking the Dairy Microbiota from Farm Bulk Tank to Skimmed Milk Powder", "description": "

Microorganisms can enter and persist in dairy at several stages of the processing chain. Detection of microorganisms within dairy food processing is currently a time-consuming and often inaccurate process. This study provides evidence that high-throughput sequencing can be used as an effective tool to accurately identify microorganisms along the processing chain. In addition, it demonstrates that the populations of microbes change from raw milk to the end product. Routine implementation of high-throughput sequencing would elucidate the factors that influence population dynamics. This will enable a manufacturer to adopt control measures specific to each stage of processing and respond in an effective manner, which would ultimately lead to increased food safety and quality.

", "keywords": ["2. Zero hunger", "0301 basic medicine", "metagenomics", "0303 health sciences", "whole-milk silo", "collection tanker", "bulk tank milk", "Microbiology", "QR1-502", "3. Good health", "03 medical and health sciences", "skimmed milk silo", "skimmed milk powder", "microbiota", "dairy", "processing", "16S rRNA gene amplicon sequencing", "Research Article"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/mSystems.00226-20"}, {"href": "https://doi.org/10.1128/msystems.00226-20"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00226-20", "name": "item", "description": "10.1128/msystems.00226-20", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00226-20"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-28T00:00:00Z"}}, {"id": "10.1128/msystems.00344-21", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2021-05-10", "title": "Network Properties of Local Fungal Communities Reveal the Anthropogenic Disturbance Consequences of Farming Practices in Vineyard Soils", "description": "

Soil fungal communities play a key role in agroecosystem sustainability. The complexity of fungal communities, at both taxonomic and functional levels, makes it difficult to find clear patterns connecting community composition with ecosystem function and to understand the impact of biotic (interspecies interactions) and abiotic (e.g., climate or anthropogenic disturbances) factors on it.

", "keywords": ["Ecolog\u00eda (Biolog\u00eda)", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "agroecosystems", "local networks", "Local networks", "Microbiolog\u00eda (Biolog\u00eda)", "579", "Ecolog\u00eda", "Emergent properties", "15. Life on land", "Microbiolog\u00eda", "fungal communities", "Microbiology", "574", "QR1-502", "Fungal communities", "03 medical and health sciences", "2401.06 Ecolog\u00eda animal", "emergent properties", "11. Sustainability", "2414 Microbiolog\u00eda", "Agroecosystems", "Research Article"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/mSystems.00344-21"}, {"href": "https://doi.org/10.1128/msystems.00344-21"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00344-21", "name": "item", "description": "10.1128/msystems.00344-21", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00344-21"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-29T00:00:00Z"}}, {"id": "10.1128/msystems.00495-19", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2020-04-13", "title": "Energetic Basis of Microbial Growth and Persistence in Desert Ecosystems", "description": "

Microbial life is surprisingly abundant and diverse in global desert ecosystems. In these environments, microorganisms endure a multitude of physicochemical stresses, including low water potential, carbon and nitrogen starvation, and extreme temperatures. In this review, we summarize our current understanding of the energetic mechanisms and trophic dynamics that underpin microbial function in desert ecosystems. Accumulating evidence suggests that dormancy is a common strategy that facilitates microbial survival in response to water and carbon limitation.

", "keywords": ["0301 basic medicine", "dormancy", "CYANOBACTERIAL EXOPOLYSACCHARIDES", "Trace gas", "Microbiology", "SOIL CRUSTS", "Energy reserve", "HIGH-AFFINITY", "03 medical and health sciences", "trace gas", "ATMOSPHERIC TRACE GASES", "Energetics", "energy reserve", "Dormancy", "SOR RONDANE MOUNTAINS", "Desert", "Photosynthesis", "106026 Ecosystem research", "CARBON-MONOXIDE", "desert", "ATACAMA DESERT", "energetics", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "photosynthesis", "COMMUNITY RESPONSE", "15. Life on land", "QR1-502", "106026 \u00d6kosystemforschung", "DRY SOIL", "13. Climate action", "MOLECULAR-HYDROGEN", "106022 Microbiology", "Minireview"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/mSystems.00495-19"}, {"href": "https://doi.org/10.1128/msystems.00495-19"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00495-19", "name": "item", "description": "10.1128/msystems.00495-19", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00495-19"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-28T00:00:00Z"}}, {"id": "10.1128/msystems.00786-20", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2021-01-11", "title": "Distribution of Mixotrophy and Desiccation Survival Mechanisms across Microbial Genomes in an Arid Biological Soil Crust Community", "description": "

This study represents a comprehensive community-wide genome-centered metagenome analysis of biological soil crust (BSC) communities in arid environments, providing insights into the distribution of genes encoding different energy generation mechanisms, as well as survival strategies, among populations in an arid soil ecosystem. It reveals the metabolic potential of several uncultured and previously unsequenced microbial genera, families, and orders, as well as differences in the metabolic potential between the most abundant BSC populations and their cultured relatives, highlighting once more the danger of inferring function on the basis of taxonomy.

", "keywords": ["0301 basic medicine", "BACTERIAL", "dormancy", "Survival", "RUBROBACTER-RADIOTOLERANS", "DIVERSITY", "Biological soil crust", "survival", "Microbiology", "7. Clean energy", "biological soil crust", "03 medical and health sciences", "mixotrophy", "Dormancy", "Mixotrophy", "SPORULATION", "COLORADO PLATEAU", "2. Zero hunger", "106022 Mikrobiologie", "metagenomics", "0303 health sciences", "ARTHROBACTER-RADIOTOLERANS", "15. Life on land", "XYLANOPHILUS", "QR1-502", "SP NOV.", "SURVIVAL", "106022 Microbiology", "RADIATION", "Metagenomics", "MEMBERS", "Research Article"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/mSystems.00786-20"}, {"href": "https://doi.org/10.1128/msystems.00786-20"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00786-20", "name": "item", "description": "10.1128/msystems.00786-20", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00786-20"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-23T00:00:00Z"}}, {"id": "10.1128/msystems.00803-19", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2020-04-20", "title": "Soil Microbial Biogeography in a Changing World: Recent Advances and Future Perspectives", "description": "

Soil microbial communities are fundamental to maintaining key soil processes associated with litter decomposition, nutrient cycling, and plant productivity and are thus integral to human well-being. Recent technological advances have exponentially increased our knowledge concerning the global ecological distributions of microbial communities across space and time and have provided evidence for their contribution to ecosystem functions. However, major knowledge gaps in soil biogeography remain to be addressed over the coming years as technology and research questions continue to evolve.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "future perspectives", "0303 health sciences", "soil microbial biogeography", "recent advances", "15. Life on land", "Microbiology", "QR1-502", "3. Good health", "03 medical and health sciences", "13. Climate action", "XXXXXX - Unknown", "Minireview"]}, "links": [{"href": "https://doi.org/10.1128/msystems.00803-19"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00803-19", "name": "item", "description": "10.1128/msystems.00803-19", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00803-19"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-28T00:00:00Z"}}, {"id": "10.1128/msystems.00859-24", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2024-09-10", "title": "A novel barcoded nanopore sequencing workflow of high-quality, full-length bacterial 16S amplicons for taxonomic annotation of bacterial isolates and complex microbial communities", "description": "ABSTRACT

Due to recent improvements, Nanopore sequencing has become a promising method for experiments relying on amplicon sequencing. We describe a flexible workflow to generate and annotate high-quality, full-length 16S rDNA amplicons. We evaluated it for two applications, namely, (i) identification of bacterial isolates and (ii) species-level profiling of microbial communities. We assessed the identification of single bacterial isolates by sequencing, using a set of barcoded full-length 16S rRNA gene primer pairs (pair A), on 47 isolates encompassing multiple genera and compared those results with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification. Species-level community profiling was tested with two sets of barcoded full-length 16S primer pairs (A and B) and compared to the results obtained with shotgun Illumina sequencing using 27 stool samples. We developed a Nextflow pipeline to retain high-quality reads and taxonomically annotate them. We found high agreement between our workflow and MALDI-TOF data for isolate identification (positive predictive value = 0.90, Cram\uffc3\uffa9r\uffe2\uff80\uff99s V = 0.857, and Theil\uffe2\uff80\uff99s U = 0.316). For species-level community profiling, we found strong correlations ( r s > 0.6) of alpha diversity indices between the two primer sets and Illumina sequencing. At the community level, we found significant but small differences when comparing sequencing techniques. Finally, we found a moderate to strong correlation when comparing the relative abundances of individual species (average r s = 0.6 and 0.533 for primers A and B). Despite identified shortcomings, the proposed workflow enabled accurate identification of single bacterial isolates and prominent features in microbial communities, making it a worthwhile alternative to MALDI-TOF MS and Illumina sequencing.

IMPORTANCE

A quick, robust, simple, and cost-effective method to identify bacterial isolates and communities in each sample is indispensable in the fields of microbiology and infection biology. Recent technological advances in Oxford Nanopore Technologies sequencing make this technique an attractive option considering the adaptability, portability, and cost-effectiveness of the platform, even with small sequencing batches. Here, we validated a flexible workflow to identify bacterial isolates and characterize bacterial communities using the Oxford Nanopore Technologies sequencing platform combined with the most recent v14 chemistry kits. For bacterial isolates, we compared our nanopore-based approach to matrix-assisted laser desorption ionization-time of flight mass spectrometry-based identification. For species-level profiling of complex bacterial communities, we compared our nanopore-based approach to Illumina shotgun sequencing. For reproducibility purposes, we wrapped the code used to process the sequencing data into a ready-to-use and self-contained Nextflow pipeline.

", "keywords": ["DNA", " Bacterial", "1303 Biochemistry", "gut microbiome", "610 Medicine & health", "Microbiology", "Workflow", "1311 Genetics", "RNA", " Ribosomal", " 16S", "1312 Molecular Biology", "1706 Computer Science Applications", "DNA Barcoding", " Taxonomic", "Humans", "DNA sequencing", "Bacteria", "10179 Institute of Medical Microbiology", "Microbiota", "2404 Microbiology", "1314 Physiology", "bioinformatics", "QR1-502", "Nanopore Sequencing", "1105 Ecology", " Evolution", " Behavior and Systematics", "Spectrometry", " Mass", " Matrix-Assisted Laser Desorption-Ionization", "570 Life sciences; biology", "2611 Modeling and Simulation", "Research Article"]}, "links": [{"href": "https://doi.org/10.1128/msystems.00859-24"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00859-24", "name": "item", "description": "10.1128/msystems.00859-24", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00859-24"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-04-11T00:00:00Z"}}, {"id": "10.1128/spectrum.01101-23", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:12Z", "type": "Journal Article", "created": "2023-09-19", "title": "Bacteriophages limitedly contribute to the antimicrobial resistome of microbial communities in wastewater treatment plants", "description": "ABSTRACT

Bacteriophages are known as players in the transmission of antimicrobial resistance genes (ARGs) by horizontal gene transfer. In this study, we characterized the bacteriophage community and the associated ARGs to estimate the potential for phages to spread ARGs in aquatic ecosystems analyzing the intra- and extracellular DNA isolated from two wastewater treatment plants (WWTPs) by shotgun metagenomics. We compared the phage antimicrobial resistome with the bacterial resistome and investigated the effect of the final disinfection treatment on the phage community and its resistome. Phage community was mainly composed by Siphoviridae and other members of the order Caudovirales . The final disinfection only marginally affected the composition of the phage community, and it was not possible to measure its effect on the antimicrobial resistome. Indeed, only three phage metagenome-assembled genomes (pMAGs) annotated as Siphoviridae , Padoviridae , and Myoviridae were positive for putative ARGs. Among the detected ARGs, i.e., dfr B6, rpo B mutants, and EF-Tu mutants, the first one was not annotated in the bacterial MAGs. Overall, these results demonstrate that bacteriophages limitedly contribute to the whole antimicrobial resistome. However, in order to obtain a comprehensive understanding of the antimicrobial resistome within a microbial community, the role of bacteriophages needs to be investigated.

IMPORTANCE

WWTPs are considered hotspots for the spread of ARGs by horizontal gene transfer. In this study, we evaluated the phage composition and the associated antimicrobial resistome by shotgun metagenomics of samples collected before and after the final disinfection treatment. Only a few bacteriophages carried ARGs. However, since one of the detected genes was not found in the bacterial metagenome-assembled genomes, it is necessary to investigate the phage community in order to gain a comprehensive overview of the antimicrobial resistome. This investigation could help assess the potential threats to human health.

", "keywords": ["metagenomics", "bacteriophages", "11. Sustainability", "Bacteriophages", "metagenomic assembled genomes", "antimicrobial resistance", "antimicrobial resistome", "wastewater treatment plants", "Microbiology", "6. Clean water", "QR1-502", "12. Responsible consumption", "Research Article"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/spectrum.01101-23"}, {"href": "https://doi.org/10.1128/spectrum.01101-23"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbiology%20Spectrum", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/spectrum.01101-23", "name": "item", "description": "10.1128/spectrum.01101-23", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/spectrum.01101-23"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-17T00:00:00Z"}}, {"id": "10.25674/so92iss2pp121", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:41Z", "type": "Journal Article", "title": "Lessons from the WBF2020: extrinsic and intrinsic value of soil organisms", "description": "Following our participation in the first World Biodiversity Forum in Davos, Switzerland, we provide a summary of the main themes of the conference, as well as an overview of the session that was focused on soil biodiversity. One of the main themes of the conference was the valuation of biodiversity and what contributes to the value of biodiversity. In this article we explore whether we should move away from the notion that we can only 'sell' soil biodiversity based on the function and services it provides, and rather shift towards valuing soil biodiversity based on its intrinsic value and our relationship with it.", "keywords": ["0301 basic medicine", "0303 health sciences", "ecosystem functions and services", "Biodiversity", "15. Life on land", "Microbiology", "QR1-502", "03 medical and health sciences", "QL1-991", "13. Climate action", "intrinsic value", "world biodiversity forum", "Zoology", "valuing soil biodiversity", "Taxonomy"]}, "links": [{"href": "https://doi.org/10.25674/so92iss2pp121"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20organisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.25674/so92iss2pp121", "name": "item", "description": "10.25674/so92iss2pp121", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.25674/so92iss2pp121"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2025.1372302", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:49Z", "type": "Journal Article", "created": "2025-02-25", "title": "Bio-electrosynthesis of polyhydroxybutyrate and surfactants in microbial fuel cells: a preliminary study", "description": "

Microbial Electrochemical Technology (MET) offers a promising avenue for CO2 utilization by leveraging the ability of chemolithotrophic microorganisms to use inorganic carbon in biosynthetic processes. By harnessing the power of electroactive bacteria, METs can facilitate the conversion of inorganic carbon into organic compounds. Therefore, this work combines biosurfactant production at the anode and PHB production at the cathode of Microbial Fuel Cells (MFCs), while testing the efficiency of Microbial Electrosynthesis Cells (MECs), and traditional culture in liquid media. This study employed a consortium of Pseudomonas aeruginosa PA1430/CO1 and Shewanella oneidensis MR-1, to provide reducing equivalents to Cupriavidus necator DSM428 for CO2 fixation and polyhydroxybutyrate (PHB) production. Glycerol was used as a carbon source by the anode consortium to investigate biosurfactant production. Additionally, Adaptive Laboratory Evolution (ALE) was employed to enhance the efficiency of this process to develop biofilms capable of synthesizing PHB from CO2 in MFCs under a controlled gas atmosphere (10% CO2, 10% O2, 2% H2, 78% N2). Observed results showed a higher direct CO2 removal from the gas mix in MECs (73%) than in MFCs (65%) compared to control cultures. Anionic (18.8 mg/L) and non-ionic (14.6 mg/L) surfactants were primarily present at the anodes of MFCs. Confocal microscope analysis revealed that the accumulation of PHBs in C. necator was significantly higher in MFCs (73% of cell volume) rather than in MECs (23%) and control cultures (40%). Further analyses on metabolites in the different systems are ongoing. Our data gave evidence that the anode consortium was able to provide enough electrons to sustain the chemolithotrophic growth of C. necator and the biosynthesis of PHBs at the cathode of MFCs, in a mechanism suggestive of the direct interspecies electron transfer (DIET), naturally occurring in natural environment.Alzheimer’s disease (AD) is a chronic neurodegenerative disease associated with the overproduction and accumulation of amyloid-β peptide and hyperphosphorylation of tau proteins in the brain. Despite extensive research on the amyloid-based mechanism of AD pathogenesis, the underlying cause of AD remains poorly understood. No disease-modifying therapies currently exist, and numerous clinical trials have failed to demonstrate any benefits. The recent discovery that the amyloid-β peptide has antimicrobial activities supports the possibility of an infectious aetiology of AD and suggests that amyloid-β plaque formation might be induced by infection. AD patients have a weakened blood-brain barrier and immune system and are thus at elevated risk of microbial infections. Such infections can cause chronic neuroinflammation, production of the antimicrobial amyloid-β peptide, and neurodegeneration. Various pathogens, including viruses, bacteria, fungi, and parasites have been associated with AD. Most research in this area has focused on individual pathogens, with herpesviruses and periodontal bacteria being most frequently implicated. The purpose of this review is to highlight the potential role of multi-pathogen infections in AD. Recognition of the potential coexistence of multiple pathogens and biofilms in AD's aetiology may stimulate the development of novel approaches to its diagnosis and treatment. Multiple diagnostic tests could be applied simultaneously to detect major pathogens, followed by anti-microbial treatment using antiviral, antibacterial, antifungal, and anti-biofilm agents.

", "keywords": ["0301 basic medicine", "Bacteria", "Review", "Antifungal", "Microbiology", "Antiviral Agents", "QR1-502", "3. Good health", "Antibacterial", "Anti-biofilm", "03 medical and health sciences", "0302 clinical medicine", "Anti-Infective Agents", "Alzheimer Disease", "Biofilms", "Animals", "Humans", "Antiviral", "Alzheimer\u2019s disease"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1186/s12934-021-01520-7.pdf"}, {"href": "https://doi.org/10.1186/s12934-021-01520-7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Cell%20Factories", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s12934-021-01520-7", "name": "item", "description": "10.1186/s12934-021-01520-7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s12934-021-01520-7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-11T00:00:00Z"}}, {"id": "10.1186/s13568-024-01764-7", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:22Z", "type": "Journal Article", "created": "2024-09-28", "title": "Metagenomic analyses of a consortium for the bioremediation of hydrocarbons polluted soils", "description": "Abstract

A bacterial consortium was isolated from a soil in Noblejas (Toledo, Spain) with a long history of mixed hydrocarbons pollution, by enrichment cultivation. Serial cultures of hydrocarbons polluted soil samples were grown in a minimal medium using diesel (1\uffc2\uffa0mL/L) as the sole carbon and energy source. The bacterial composition of the Noblejas Consortium (NC) was determined by sequencing 16S rRNA gene amplicon libraries. The consortium contained around 50 amplicon sequence variants (ASVs) and the major populations belonged to the genera Pseudomonas, Enterobacter, Delftia, Stenotrophomonas, Achromobacter, Acinetobacter, Novosphingobium, Allorhizobium-Neorhizobium-Rhizobium, Ochrobactrum and Luteibacter. All other genera were below 1%. Metagenomic analysis of NC has shown a high abundance of genes encoding enzymes implicated in aliphatic and (poly) aromatic hydrocarbons degradation, and almost all pathways for hydrocarbon degradation are represented. Metagenomic analysis has also allowed the construction of metagenome assembled genomes (MAGs) for the major players of NC. Metatranscriptomic analysis has shown that several of the ASVs are implicated in hydrocarbon degradation, being Pseudomonas, Acinetobacter and Delftia the most active populations.There is an increase in the adoption of biological solutions for plant production as a means of attaining sustainable agriculture. A detailed understanding of the influence of specific bioinoculants and their volatile metabolites on native soil and plant microbiomes can improve future microbiome management practices.

Results

Here, we examined the effect of bacterial inoculants and volatile compounds as individual and combined treatments on apple plant and soil microbiome. The study used specially designed microcosms that facilitated the separation of the different plant compartments. A compartment- and soil-specific effect of treatments on the native soil and plant microbiome was observed. The live bacterial inoculants as compared to their volatiles had a stronger effect on the plant and soil microbiome, particularly the root microbial community. The combined effect of bacterial inoculants was higher compared to volatiles (R2\uffe2\uff80\uff89=\uffe2\uff80\uff895% vs. 3%). Treatment-specific effects were observed, like the influence of 2-butanone on the phyllosphere bacterial diversity, and an increase in fungal richness in Serratia-treated soils.

Conclusions

Among the examined treatments, inoculation with bacteria compared to volatile metabolites induced more significant shifts within the plant and soil microbiome. This observation has implications regarding the merits of applying living microorganisms. The findings highlight the potential of microbiome management approaches for enhancing microbiota functions.

", "keywords": ["Microcosm experiments", "Environmental sciences", "Plant microbiome", "Research", "Sustainable agriculture", "GE1-350", "Volatile organic compounds", "Microbiology", "Bioinoculants", "QR1-502", "Amplicon sequencing"]}, "links": [{"href": "https://doi.org/10.1186/s40793-025-00715-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-025-00715-4", "name": "item", "description": "10.1186/s40793-025-00715-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-025-00715-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-05-21T00:00:00Z"}}, {"id": "10.21203/rs.3.rs-25108/v1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:05Z", "type": "Journal Article", "created": "2020-05-01", "title": "Trichomes form genotype-specific microbial hotspots in the phyllosphere of tomato", "description": "Abstract

Background The plant phyllosphere is a well-studied habitat characterized by low nutrient availability and high community dynamics. In contrast, plant trichomes, known for their production of a large number of metabolites, are a yet unexplored habitat for microbes. We analyzed the phyllosphere as well as trichomes of two tomato genotypes (Solanum lycopersicum LA4024, S. habrochaites LA1777) by targeting bacterial 16S rRNA gene fragments.Results Leaves, leaves without trichomes, and trichomes alone harbored similar abundances of bacteria (108- 109 16S rRNA gene copy numbers per gram of sample). In contrast, bacterial diversity was found significantly increased in trichome samples (Shannon index: 4.4 vs. 2.5). Moreover, the community composition was significantly different when assessed with beta diversity analysis and corresponding statistical tests. At the bacterial class level, Alphaproteobacteria (23.6%) were significantly increased, whereas Bacilli (8.6%) were decreased in trichomes. The bacterial family Sphingomonadacea (8.4%) was identified as the most prominent, trichome-specific taxa; Burkholderiaceae and Actinobacteria showed similar pattern. Moreover, Sphingomonas was identified as a central element in the core microbiome of trichome samples, while distinct low-abundant bacterial families including Hymenobacteraceae and Alicyclobacillaceae were exclusively found in trichome samples. Niche preferences were statistically significant for both genotypes and genotype-specific enrichments were further observed.Conclusion Our results provide first evidence of a highly specific trichome microbiome in tomato and show the importance of micro-niches for the structure of bacterial communities on leaves. These findings provide further clues for breeding, plant pathology and protection as well as so far unexplored natural pathogen defense strategies.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "Plant microhabitat", "0303 health sciences", "Plant microbiome", "Trichomes", "15. Life on land", "Microbiology", "Bacterial communities", "QR1-502", "Environmental sciences", "03 medical and health sciences", "Solanum lycopersicum", "Solanum habrochaites", "plant microbiome", " bacterial communities", " plant-microbe interactions", " Solanum habrochaites", " Solanum lycopersicum", " plant microhabitat", " phyllosphere", " trichomes", "GE1-350", "Phyllosphere", "Plant-microbe interactions", "Research Article"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1186/s40793-020-00364-9.pdf"}, {"href": "https://doi.org/10.21203/rs.3.rs-25108/v1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.21203/rs.3.rs-25108/v1", "name": "item", "description": "10.21203/rs.3.rs-25108/v1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.21203/rs.3.rs-25108/v1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-01T00:00:00Z"}}, {"id": "10.25674/so91200", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:41Z", "type": "Journal Article", "title": "Soil Organisms - an international open access journal on the taxonomic and functional biodiversity in the soil", "description": "Biodiversity change", "keywords": ["open access", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Biodiversity", "soil organisms", "15. Life on land", "Microbiology", "QR1-502", "03 medical and health sciences", "QL1-991", "13. Climate action", "Zoology", "Taxonomy"]}, "links": [{"href": "https://doi.org/10.25674/so91200"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20organisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.25674/so91200", "name": "item", "description": "10.25674/so91200", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.25674/so91200"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-01-01T00:00:00Z"}}, {"id": "10.25674/so91201", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:41Z", "type": "Journal Article", "title": "Testing soil nematode extraction efficiency using different variations of the Baermann-funnel method", "description": "soil organisms | comparability | reproducibility | extraction methods", "keywords": ["extraction methods", "QL1-991", "comparability", "Biodiversity", "soil organisms", "15. Life on land", "reproducibility", "Microbiology", "Zoology", "QR1-502", "Taxonomy"]}, "links": [{"href": "https://doi.org/10.25674/so91201"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20organisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.25674/so91201", "name": "item", "description": "10.25674/so91201", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.25674/so91201"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-01-01T00:00:00Z"}}, {"id": "10.25674/362", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:41Z", "type": "Journal Article", "title": "Soil BON Earthworm - A global initiative on earthworm distribution, traits, and spatiotemporal diversity patterns", "description": "Open AccessPeer reviewed", "keywords": ["2. Zero hunger", "temporal dynamics", "500", "soil biodiversity", "Biodiversity", "earthworms", "time-series data", "15. Life on land", "Traits", "Microbiology", "630", "QR1-502", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "QL1-991", "Ecology", " evolutionary biology", "global collaboration", "ecosystem functioning", "citizen science", "Community ecology", "functional traits", "14. Life underwater", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "Zoology", "community ecology", "Taxonomy"]}, "links": [{"href": "https://doi.org/10.25674/362"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Organisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.25674/362", "name": "item", "description": "10.25674/362", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.25674/362"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.25674/413", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:41Z", "type": "Journal Article", "title": "Communicating soil biodiversity research to kids around the world", "description": "Open AccessPeer reviewed", "keywords": ["[SDE] Environmental Sciences", "young adults", "2. Zero hunger", "570", "education", "4. Education", "translation", "online resource", "soil biodiversity", "Biodiversity", "Media and communications", "15. Life on land", "science communication", "Microbiology", "333", "teaching", "QR1-502", "children", "QL1-991", "Ecology", " evolutionary biology", "13. Climate action", "[SDE]Environmental Sciences", "11. Sustainability", "Zoology", "Taxonomy"]}, "links": [{"href": "https://doi.org/10.25674/413"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Organisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.25674/413", "name": "item", "description": "10.25674/413", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.25674/413"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2016.01207", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2016-08-03", "title": "Wheat And Rice Growth Stages And Fertilization Regimes Alter Soil Bacterial Community Structure, But Not Diversity", "description": "Maintaining soil fertility and the microbial communities that determine fertility is critical to sustainable agricultural strategies, and the use of different organic fertilizer (OF) regimes represents an important practice in attempts to preserve soil quality. However, little is known about the dynamic response of bacterial communities to fertilization regimes across crop growth stages. In this study, we examined microbial community structure and diversity across eight representative growth stages of wheat-rice rotation under four different fertilization treatments: no nitrogen fertilizer (NNF), chemical fertilizer (CF), organic-inorganic mixed fertilizer (OIMF), and OF. Quantitative PCR (QPCR) and high-throughput sequencing of bacterial 16S rRNA gene fragments revealed that growth stage as the best predictor of bacterial community abundance and structure. Additionally, bacterial community compositions differed between wheat and rice rotations. Relative to soils under wheat rotation, soils under rice rotation contained higher relative abundances (RA) of anaerobic and mesophilic microbes and lower RA of aerophilic microbes. With respect to fertilization regime, NNF plots had a higher abundance of nitrogen-fixing Cyanobacteria. OIMF had a lower abundance of ammonia-oxidizing Thaumarchaeota compared with CF. Application of chemical fertilizers (CF and OIMF treatments) significantly increased the abundance of some generally oligotrophic bacteria such those belonging to the Acidobacteria, while more copiotrophic of the phylum Proteobacteria increased with OF application. A high correlation coefficient was found when comparing RA of Acidobacteria based upon QPCR vs. sequence analysis, yet poor correlations were found for the \u03b1- and \u03b2- Proteobacteria, highlighting the caution required when interpreting these molecular data. In total, crop, fertilization scheme and plant developmental stage all influenced soil microbial community structure, but not total levels of alpha diversity.", "keywords": ["2. Zero hunger", "Growth stage", "Fertilization regime", "04 agricultural and veterinary sciences", "15. Life on land", "bacterial community", "Microbiology", "Bacterial communities", "QR1-502", "growth stage", "fertilization regime", "wheat-rice rotation system", "0401 agriculture", " forestry", " and fisheries", "dynamic variation", "Wheat-rice rotation system", "Dynamic variation"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2016.01207"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.01207", "name": "item", "description": "10.3389/fmicb.2016.01207", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.01207"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-08-03T00:00:00Z"}}, {"id": "10.3389/fmicb.2018.01176", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2018-06-08", "title": "Enhanced Bacterial Fitness Under Residual Fluoroquinolone Concentrations Is Associated With Increased Gene Expression in Wastewater-Derived qnr Plasmid-Harboring Strains", "description": "Plasmids harboring qnr genes confer resistance to low fluoroquinolone concentrations. These genes are of significant clinical, evolutionary and environmental importance, since they are widely distributed in a diverse array of natural and clinical environments. We previously extracted and sequenced a large (\u223c185 Kbp) qnrB-harboring plasmid, and several small (\u223c8 Kbp) qnrS-harboring plasmids, from Klebsiella pneumoniae isolates from municipal wastewater biosolids, and hypothesized that these plasmids provide host bacteria a selective advantage in wastewater treatment plants (WWTPs) that often contain residual concentrations of fluoroquinolones. The objectives of this study were therefore to determine the effect of residual fluoroquinolone concentrations on the growth kinetics of qnr plasmid-harboring bacteria; and on the copy number of qnr plasmids and expression of qnr genes. Electrotransformants harboring either one of the two types of plasmids could grow at ciprofloxacin concentrations exceeding 0.5 \u03bcg ml-1, but growth was significantly decreased at concentrations higher than 0.1 \u03bcg ml-1. In contrast, plasmid-free strains failed to grow even at 0.05 \u03bcg ml-1. No differences were observed in plasmid copy number under the tested ciprofloxacin concentrations, but qnr expression increased incrementally from 0 to 0.4 \u03bcg ml-1, suggesting that the transcription of this gene is regulated by antibiotic concentration. This study reveals that wastewater-derived qnr plasmids confer a selective advantage in the presence of residual fluoroquinolone concentrations and provides a mechanistic explanation for this phenomenon.", "keywords": ["0301 basic medicine", "0303 health sciences", "qnr genes", "Microbiology", "QR1-502", "6. Clean water", "fitness", "3. Good health", "wastewater treatment", "03 medical and health sciences", "qPCR expression analysis", "plasmid", "11. Sustainability", "qnr genes", " wastewater treatment", " plasmid", " qPCR expression analysis", " fitness"], "contacts": [{"organization": "Roberto B. M. Marano, Roberto B. M. Marano, Edouard Jurkevitch, Eddie Cytryn, Ella Kaplan, Ella Kaplan,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2018.01176"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2018.01176", "name": "item", "description": "10.3389/fmicb.2018.01176", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2018.01176"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-06-08T00:00:00Z"}}, {"id": "10.3389/fmicb.2018.02141", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2018-09-12", "title": "Pierce's Disease of Grapevines: A Review of Control Strategies and an Outline of an Epidemiological Model", "description": "Xylella fastidiosa is a notorious plant pathogenic bacterium that represents a threat to crops worldwide. Its subspecies, Xylella fastidiosa subsp. fastidiosa is the causal agent of Pierce's disease of grapevines. Pierce's disease has presented a serious challenge for the grapevine industry in the United States and turned into an epidemic in Southern California due to the invasion of the insect vector Homalodisca vitripennis. In an attempt to minimize the effects of Xylella fastidiosa subsp. fastidiosa in vineyards, various studies have been developing and testing strategies to prevent the occurrence of Pierce's disease, i.e., prophylactic strategies. Research has also been undertaken to investigate therapeutic strategies to cure vines infected by Xylella fastidiosa subsp. fastidiosa. This report explicitly reviews all the strategies published to date and specifies their current status. Furthermore, an epidemiological model of Xylella fastidiosa subsp. fastidiosa is proposed and key parameters for the spread of Pierce's disease deciphered in a sensitivity analysis of all model parameters. Based on these results, it is concluded that future studies should prioritize therapeutic strategies, while investments should only be made in prophylactic strategies that have demonstrated promising results in vineyards.", "keywords": ["VECTOR TRANSMISSION", "0301 basic medicine", "VITRIPENNIS HEMIPTERA CICADELLIDAE", "GLASSY-WINGED SHARPSHOOTER", "PARATRANSGENIC CONTROL", "epidemiological model", "control strategies", "BACTERIUM XYLELLA-FASTIDIOSA", "Microbiology", "03 medical and health sciences", "HOT-WATER TREATMENT", "GONATOCERUS-ASHMEADI", "MOLECULAR CHARACTERIZATION", "Pierce's disease", "Xylella fastidiosa", "2. Zero hunger", "HOMALODISCA-COAGULATA HEMIPTERA", "0303 health sciences", "VITIS-VINIFERA L.", "15. Life on land", "QR1-502", "grapevine", "3. Good health", "[SDV] Life Sciences [q-bio]", "therapeutic", "prophylactic", "Homalodisca vitripennis", "[INFO.INFO-MO] Computer Science [cs]/Modeling and Simulation"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2018.02141"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2018.02141", "name": "item", "description": "10.3389/fmicb.2018.02141", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2018.02141"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-12T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.00168", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2019-02-26", "title": "Rapid Transfer of Plant Photosynthates to Soil Bacteria via Ectomycorrhizal Hyphae and Its Interaction With Nitrogen Availability", "description": "Plant roots release recent photosynthates into the rhizosphere, accelerating decomposition of organic matter by saprotrophic soil microbes ('rhizosphere priming effect') which consequently increases nutrient availability for plants. However, about 90% of all higher plant species are mycorrhizal, transferring a significant fraction of their photosynthates directly to their fungal partners. Whether mycorrhizal fungi pass on plant-derived carbon (C) to bacteria in root-distant soil areas, i.e., incite a 'hyphosphere priming effect,' is not known. Experimental evidence for C transfer from mycorrhizal hyphae to soil bacteria is limited, especially for ectomycorrhizal systems. As ectomycorrhizal fungi possess enzymatic capabilities to degrade organic matter themselves, it remains unclear whether they cooperate with soil bacteria by providing photosynthates, or compete for available nutrients. To investigate a possible C transfer from ectomycorrhizal hyphae to soil bacteria, and its response to changing nutrient availability, we planted young beech trees (Fagus sylvatica) into 'split-root' boxes, dividing their root systems into two disconnected soil compartments. Each of these compartments was separated from a litter compartment by a mesh penetrable for fungal hyphae, but not for roots. Plants were exposed to a 13C-CO2-labeled atmosphere, while 15N-labeled ammonium and amino acids were added to one side of the split-root system. We found a rapid transfer of recent photosynthates via ectomycorrhizal hyphae to bacteria in root-distant soil areas. Fungal and bacterial phospholipid fatty acid (PLFA) biomarkers were significantly enriched in hyphae-exclusive compartments 24 h after 13C-CO2-labeling. Isotope imaging with nanometer-scale secondary ion mass spectrometry (NanoSIMS) allowed for the first time in situ visualization of plant-derived C and N taken up by an extraradical fungal hypha, and in microbial cells thriving on hyphal surfaces. When N was added to the litter compartments, bacterial biomass, and the amount of incorporated 13C strongly declined. Interestingly, this effect was also observed in adjacent soil compartments where added N was only available for bacteria through hyphal transport, indicating that ectomycorrhizal fungi were acting on soil bacteria. Together, our results demonstrate that (i) ectomycorrhizal hyphae rapidly transfer plant-derived C to bacterial communities in root-distant areas, and (ii) this transfer promptly responds to changing soil nutrient conditions.", "keywords": ["Hyphosphere priming", "DYNAMICS", "0301 basic medicine", "PLFAs", "Microbiology", "ectomycorrhiza", "03 medical and health sciences", "Mycorrhizosphere", "MICROBIAL COMMUNITY COMPOSITION", "NanoSIMS", "hyphal carbon transfer", "hyphosphere bacteria", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "IDENTIFICATION", "RHIZOSPHERE", "15. Life on land", "QR1-502", "EXTRACTION METHOD", "Ectomycorrhiza", "ORGANIC-MATTER", "MYCORRHIZAL FUNGI", "hyphosphere priming", "mycorrhizosphere", "Hyphal carbon transfer", "106022 Microbiology", "FATTY-ACIDS", "Hyphosphere bacteria", "BAYESIAN CLASSIFIER", "CARBON ALLOCATION"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.00168"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.00168", "name": "item", "description": "10.3389/fmicb.2019.00168", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.00168"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-26T00:00:00Z"}}, {"id": "10.3389/fmicb.2024.1505220", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:49Z", "type": "Journal Article", "created": "2024-10-30", "title": "Editorial: The impact of environmentally friendly agricultural practices on soil microbiome", "keywords": ["VOCs; biocontrol; biofertilizer; fungi; plant growth-promoting rhizobacteria (PGPR)", "biofertilizer", "VOCs", "biocontrol", "fungi", "plant growth-promoting rhizobacteria (PGPR)", "Microbiology", "QR1-502"], "contacts": [{"organization": "Loredana Canfora, Massimo Pugliese, Massimo Pugliese, Ewa Maria Furmanczyk,", "roles": ["creator"]}]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/2041377/1/fmicb-15-1505220.pdf"}, {"href": "https://doi.org/10.3389/fmicb.2024.1505220"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2024.1505220", "name": "item", "description": "10.3389/fmicb.2024.1505220", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2024.1505220"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-30T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.00697", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:48Z", "type": "Journal Article", "created": "2019-04-09", "title": "Taxonomic and Functional Characterization of the Microbial Community During Spontaneous in vitro Fermentation of Riesling Must", "description": "Although there is an extensive tradition of research into the microbes that underlie the winemaking process, much remains to be learnt. We combined the high-throughput sequencing (HTS) tools of metabarcoding and metagenomics, to characterize how microbial communities of Riesling musts sampled at four different vineyards, and their subsequent spontaneously fermented derivatives, vary. We specifically explored community variation relating to three points: (i) how microbial communities vary by vineyard; (ii) how community biodiversity changes during alcoholic fermentation; and (iii) how microbial community varies between musts that successfully complete alcoholic fermentation and those that become 'stuck' in the process. Our metabarcoding data showed a general influence of microbial composition at the vineyard level. Two of the vineyards (4 and 5) had strikingly a change in the differential abundance of Metschnikowia. We therefore additionally performed shotgun metagenomic sequencing on a subset of the samples to provide preliminary insights into the potential relevance of this observation, and used the data to both investigate functional potential and reconstruct draft genomes (bins). At these two vineyards, we also observed an increase in non-Saccharomycetaceae fungal functions, and a decrease in bacterial functions during the early fermentation stage. The binning results yielded 11 coherent bins, with both vineyards sharing the yeast bins Hanseniaspora and Saccharomyces. Read recruitment and functional analysis of this data revealed that during fermentation, a high abundance of Metschnikowia might serve as a biocontrol agent against bacteria, via a putative iron depletion pathway, and this in turn could help Saccharomyces dominate the fermentation. During alcoholic fermentation, we observed a general decrease in biodiversity in both the metabarcoding and metagenomic data. Unexpected Micrococcus behavior was observed in vineyard 4 according to metagenomic analyses based on reference-based read mapping. Analysis of open reading frames using these data showed an increase of functions assigned to class Actinobacteria in the end of fermentation. Therefore, we hypothesize that bacteria might sit-and-wait until Saccharomyces activity slows down. Complementary approaches to annotation instead of relying a single database provide more coherent information true species. Lastly, our metabarcoding data enabled us to identify a relationship between stuck fermentations and Starmerella abundance. Given that robust chemical analysis indicated that although the stuck samples contained residual glucose, all fructose had been consumed, we hypothesize that this was because fructophilic Starmerella, rather than Saccharomyces, dominated these fermentations. Overall, our results showcase the different ways in which metagenomic analyses can improve our understanding of the wine alcoholic fermentation process.", "keywords": ["2. Zero hunger", "0301 basic medicine", "Microbial diversity", "0303 health sciences", "Wine", "15. Life on land", "shotgun sequencing", "Microbiology", "Shotgun sequencing", "QR1-502", "03 medical and health sciences", "alcoholic fermentation", "microbial diversity", "metabarcoding", "Metabarcoding", "Alcoholic fermentation", "wine", "SDG 2 - Zero Hunger"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.00697"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.00697", "name": "item", "description": "10.3389/fmicb.2019.00697", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.00697"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-09T00:00:00Z"}}, {"id": "10.3389/fmicb.2013.00078", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:47Z", "type": "Journal Article", "created": "2013-04-09", "title": "Changes In Fungal Community Composition In Response To Elevated Atmospheric Co2 And Nitrogen Fertilization Varies With Soil Horizon", "description": "Increasing levels of atmospheric carbon dioxide (CO2) and rates of nitrogen (N)-deposition to forest ecosystems are predicted to alter the structure and function of soil fungal communities, but the spatially heterogeneous distribution of soil fungi has hampered investigations aimed at understanding such impacts. We hypothesized that soil physical and chemical properties and fungal community composition would be differentially impacted by elevated atmospheric CO2 (eCO2) and N-fertilization in spatially separated field samples, in the forest floor, 0-2, 2-5, and 5-10\u2009cm depth intervals in a loblolly pine Free-Air Carbon Dioxide Enrichment (FACE) experiment. In all soils, quantitative PCR-based estimates of fungal biomass were highest in the forest floor. Fungal richness, based on pyrosequencing of the fungal ribosomal large subunit gene, increased in response to N-fertilization in 0-2\u2009cm and forest floor intervals. Composition shifted in forest floor, 0-2 and 2-5\u2009cm intervals in response to N-fertilization, but the shift was most distinct in the 0-2\u2009cm interval, in which the largest number of statistically significant changes in soil chemical parameters (i.e., phosphorus, organic matter, calcium, pH) was also observed. In the 0-2\u2009cm interval, increased recovery of sequences from the Thelephoraceae, Tricholomataceae, Hypocreaceae, Clavicipitaceae, and Herpotrichiellaceae families and decreased recovery of sequences from the Amanitaceae correlated with N-fertilization. In this same depth interval, Amanitaceae, Tricholomataceae, and Herpotriciellaceae sequences were recovered less frequently from soils exposed to eCO2 relative to ambient conditions. These results demonstrated that vertical stratification should be taken into consideration in future efforts to elucidate environmental impacts on fungal communities and their feedbacks on ecosystem processes.", "keywords": ["elevated CO2", "soil chemistry", "basidiomycota", "Basidiomycota", "nitrogen fertilization", "04 agricultural and veterinary sciences", "Soil Chemistry", "15. Life on land", "agaricales", "Microbiology", "QR1-502", "forest floor", "Ascomycota", "13. Climate action", "soil fungi", "0401 agriculture", " forestry", " and fisheries", "Agaricales"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2013.00078"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2013.00078", "name": "item", "description": "10.3389/fmicb.2013.00078", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2013.00078"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-01-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2013.00146", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:47Z", "type": "Journal Article", "created": "2013-06-11", "description": "The activities of extracellular enzymes, the proximate agents of decomposition in soils, are known to depend strongly on temperature, but less is known about how they respond to changes in precipitation patterns, and the interaction of these two components of climate change. Both enzyme production and turnover can be affected by changes in temperature and soil moisture, thus it is difficult to predict how enzyme pool size may respond to altered climate. Soils from the Boston-Area Climate Experiment (BACE), which is located in an old field (on abandoned farmland), were used to examine how climate variables affect enzyme activities and microbial biomass carbon (MBC) in different seasons and in soils exposed to a combination of three levels of precipitation treatments (ambient, 150% of ambient during growing season, and 50% of ambient year-round) and four levels of warming treatments (unwarmed to ~4\u00b0C above ambient) over the course of a year. Warming, precipitation and season had very little effect on potential enzyme activity. Most models assume that enzyme dynamics follow microbial biomass, because enzyme production should be directly controlled by the size and activity of microbial biomass. We observed differences among seasons and treatments in mass-specific potential enzyme activity, suggesting that this assumption is invalid. In June 2009, mass-specific potential enzyme activity, using chloroform fumigation-extraction MBC, increased with temperature, peaking under medium warming and then declining under the highest warming. This finding suggests that either enzyme production increased with temperature or turnover rates decreased. Increased maintenance costs associated with warming may have resulted in increased mass-specific enzyme activities due to increased nutrient demand. Our research suggests that allocation of resources to enzyme production could be affected by climate-induced changes in microbial efficiency and maintenance costs.", "keywords": ["2. Zero hunger", "570", "decomposition", "550", "microbial biomass", "Nitrogen", "carbon", "enzymes", "temperature", "04 agricultural and veterinary sciences", "precipitation", "15. Life on land", "Microbiology", "nitrogen", "Carbon", "QR1-502", "6. Clean water", "Enzymes", "13. Climate action", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2013.00146"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2013.00146", "name": "item", "description": "10.3389/fmicb.2013.00146", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2013.00146"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-01-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2014.00516", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:47Z", "type": "Journal Article", "created": "2014-10-02", "title": "Soil Bacterial Community Composition Altered By Increased Nutrient Availability In Arctic Tundra Soils", "description": "The pool of soil organic carbon (SOC) in the Arctic is disproportionally large compared to those in other biomes. This large quantity of SOC accumulated over millennia due to slow rates of decomposition relative to net primary productivity. Decomposition is constrained by low temperatures and nutrient concentrations, which limit soil microbial activity. We investigated how nutrients limit bacterial and fungal biomass and community composition in organic and mineral soils within moist acidic tussock tundra ecosystems. We sampled two experimental arrays of moist acidic tussock tundra that included fertilized and non-fertilized control plots. One array included plots that had been fertilized annually since 1989 and the other since 2006. Fertilization significantly altered overall bacterial community composition and reduced evenness, to a greater degree in organic than mineral soils, and in the 1989 compared to the 2006 site. The relative abundance of copiotrophic \u03b1-Proteobacteria and \u03b2-Proteobacteria was higher in fertilized than control soils, and oligotrophic Acidobacteria were less abundant in fertilized than control soils at the 1989 site. Fungal community composition was less sensitive to increased nutrient availability, and fungal responses to fertilization were not consistent between soil horizons and sites. We detected two ectomycorrhizal genera, Russula and Cortinarius spp., associated with shrubs. Their relative abundance was not affected by fertilization despite increased dominance of their host plants in the fertilized plots. Our results indicate that fertilization, which has been commonly used to simulate warming in Arctic tundra, has limited applicability for investigating fungal dynamics under warming.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "550", "Bacteria", "Nitrogen", "Fungi", "Phosphorus", "15. Life on land", "Microbiology", "nitrogen", "QR1-502", "soil", "03 medical and health sciences", "fertilization", "13. Climate action", "Fertilization", "Mycorrhizae", "fungi", "Arctic tundra", "phosphorus", "bacteria", "mycorrhizae"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2014.00516"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2014.00516", "name": "item", "description": "10.3389/fmicb.2014.00516", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2014.00516"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-10-02T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR1-502&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR1-502&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR1-502&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=QR1-502&offset=50", "hreflang": "en-US"}], "numberMatched": 156, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-07-26T05:19:37.234551Z"}