{"type": "FeatureCollection", "features": [{"id": "10.1038/s41598-023-49194-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:16:49Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "Abstract

Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.

The genus Pseudomonas comprises many known plant-associated microbes with plant growth promotion and disease suppression properties. Genome-based studies allow the prediction of the underlying mechanisms using genome mining tools and the analysis of the genes unique for a strain by implementing comparative genomics. Here, we provide the genome sequence of the strain Pseudomonas brassicacearum 3Re2-7, formerly known as P. trivialis and P. reactans, elucidate its revised taxonomic classification, experimentally verify the gene predictions by transcriptome sequencing, describe its genetic biocontrol potential and contextualize it to other known Pseudomonas biocontrol agents. The P. brassicacearum 3Re2-7 genome comprises a circular chromosome with a size of 6,738,544 bp and a GC-content of 60.83%. 6267 genes were annotated, of which 6113 were shown to be transcribed in rich medium and/or in the presence of Rhizoctonia solani. Genome mining identified genes related to biocontrol traits such as secondary metabolite and siderophore biosynthesis, plant growth promotion, inorganic phosphate solubilization, biosynthesis of lipo- and exopolysaccharides, exoproteases, volatiles and detoxification. Core genome analysis revealed, that the 3Re2-7 genome exhibits a high collinearity with the representative genome for the species, P. brassicacearum subsp. brassicacearum NFM421. Comparative genomics allowed the identification of 105 specific genes and revealed gene clusters that might encode specialized biocontrol mechanisms of strain 3Re2-7. Moreover, we captured the transcriptome of P. brassicacearum 3Re2-7, confirming the transcription of the predicted biocontrol-related genes. The gene clusters coding for 2,4-diacetylphloroglucinol (phlABCDEFGH) and hydrogen cyanide (hcnABC) were shown to be highly transcribed. Further genes predicted to encode putative alginate production enzymes, a pyrroloquinoline quinone precursor peptide PqqA and a matrixin family metalloprotease were also found to be highly transcribed. With this study, we provide a basis to further characterize the mechanisms for biocontrol in Pseudomonas species, towards a sustainable and safe application of P. brassicacearum biocontrol agents.

", "keywords": ["COMPARATIVE GENOMICS", "0301 basic medicine", "570", "Antifungal Agents", "Plant-growth promotion", "Biolog\u00eda", "comparative genomics", "Phloroglucinol", "PLANT-GROWTH PROMOTION", "Article", "Rhizoctonia", "12. Responsible consumption", "transcriptomics", "03 medical and health sciences", "https://purl.org/becyt/ford/1.6", "Genome mining", "Hydrogen Cyanide", "Pseudomonas", "genome mining", "RNA SEQUENCING", "TRANSCRIPTOMICS", "biocontrol", "GENOME MINING", "PSEUDOMONASBRASSICACEARUM", "https://purl.org/becyt/ford/1", "Transcriptomics", "2. Zero hunger", "0303 health sciences", "Comparative genomics", "Biocontrol", "RNA sequencing", "Pseudomonas brassicacearum", "3. Good health", "BIOCONTROL", "Pseudomonas brassicacearum", "Biological Control Agents", "Genes", " Bacterial", "Transcriptome", "plant-growth promotion"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://doi.org/10.3390/genes10080601"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes10080601", "name": "item", "description": "10.3390/genes10080601", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes10080601"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-09T00:00:00Z"}}, {"id": "10.3390/v12060675", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:19:33Z", "type": "Journal Article", "created": "2020-06-23", "title": "Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato", "description": "

Tomato plants can establish symbiotic interactions with arbuscular mycorrhizal fungi (AMF) able to promote plant nutrition and prime systemic plant defenses against pathogens attack; the mechanism involved is known as mycorrhiza-induced resistance (MIR). However, studies on the effect of AMF on viral infection, still limited and not conclusive, indicate that AMF colonization may have a detrimental effect on plant defenses against viruses, so that the term \uffe2\uff80\uff9cmycorrhiza-induced susceptibility\uffe2\uff80\uff9d (MIS) has been proposed for these cases. To expand the case studies to a not yet tested viral family, that is, Bromoviridae, we investigated the effect of the colonization by the AMF Funneliformis mosseae on cucumber mosaic virus (CMV) infection in tomato by phenotypic, physiological, biochemical, and transcriptional analyses. Our results showed that the establishment of a functional AM symbiosis is able to limit symptoms development. Physiological and transcriptomic data highlighted that AMF mitigates the drastic downregulation of photosynthesis-related genes and the reduction of photosynthetic CO2 assimilation rate caused by CMV infection. In parallel, an increase of salicylic acid level and a modulation of reactive oxygen species (ROS)-related genes, toward a limitation of ROS accumulation, was specifically observed in CMV-infected mycorrhizal plants. Overall, our data indicate that the AM symbiosis influences the development of CMV infection in tomato plants and exerts a priming effect able to enhance tolerance to viral infection.

", "keywords": ["0301 basic medicine", "plant\u2013virus interaction", "arbuscular mycorrhizal symbiosis", "Microbiology", "Cucumovirus", "Plant Roots", "Article", "03 medical and health sciences", "Solanum lycopersicum", "Gene Expression Regulation", " Plant", "Mycorrhizae", "arbuscular mycorrhizal symbiosis", " cucumber mosaic virus", " Funneliformis mosseae", " gene expression", " priming tolerance", " plant-virus interaction", " RNA sequencing", " Solanum lycopersicum L.", "Photosynthesis", "Symbiosis", "Funneliformis mosseae", "Plant Diseases", "2. Zero hunger", "0303 health sciences", "cucumber mosaic virus", "Fungi", "RNA sequencing", "Carbon Dioxide", "QR1-502", "3. Good health", "Solanum lycopersicum L.", "gene expression", "arbuscular mycorrhizal symbiosis; cucumber mosaic virus; Funneliformis mosseae; gene expression; priming tolerance; plant-virus interaction; RNA sequencing; Solanum lycopersicum L.", "priming tolerance", "Arbuscular mycorrhizal symbiosis; Cucumber mosaic virus; Funneliformis mosseae; Gene expression; Plant-virus interaction; Priming tolerance; RNA sequencing; Solanum lycopersicum L", "Reactive Oxygen Species"]}, "links": [{"href": "http://www.mdpi.com/1999-4915/12/6/675/pdf"}, {"href": "https://iris.cnr.it/bitstream/20.500.14243/410166/1/prod_424799-doc_151509.pdf"}, {"href": "https://iris.unito.it/bitstream/2318/1765477/1/Miozzi%20et%20al%20Viruses%202020.pdf"}, {"href": "https://www.mdpi.com/1999-4915/12/6/675/pdf"}, {"href": "https://doi.org/10.3390/v12060675"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Viruses", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/v12060675", "name": "item", "description": "10.3390/v12060675", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/v12060675"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-06-22T00:00:00Z"}}, {"id": "11336/151981", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:22:16Z", "type": "Journal Article", "created": "2019-08-09", "title": "Genetic Potential of the Biocontrol Agent Pseudomonas brassicacearum (Formerly P. trivialis) 3Re2-7 Unraveled by Genome Sequencing and Mining, Comparative Genomics and Transcriptomics", "description": "

The genus Pseudomonas comprises many known plant-associated microbes with plant growth promotion and disease suppression properties. Genome-based studies allow the prediction of the underlying mechanisms using genome mining tools and the analysis of the genes unique for a strain by implementing comparative genomics. Here, we provide the genome sequence of the strain Pseudomonas brassicacearum 3Re2-7, formerly known as P. trivialis and P. reactans, elucidate its revised taxonomic classification, experimentally verify the gene predictions by transcriptome sequencing, describe its genetic biocontrol potential and contextualize it to other known Pseudomonas biocontrol agents. The P. brassicacearum 3Re2-7 genome comprises a circular chromosome with a size of 6,738,544 bp and a GC-content of 60.83%. 6267 genes were annotated, of which 6113 were shown to be transcribed in rich medium and/or in the presence of Rhizoctonia solani. Genome mining identified genes related to biocontrol traits such as secondary metabolite and siderophore biosynthesis, plant growth promotion, inorganic phosphate solubilization, biosynthesis of lipo- and exopolysaccharides, exoproteases, volatiles and detoxification. Core genome analysis revealed, that the 3Re2-7 genome exhibits a high collinearity with the representative genome for the species, P. brassicacearum subsp. brassicacearum NFM421. Comparative genomics allowed the identification of 105 specific genes and revealed gene clusters that might encode specialized biocontrol mechanisms of strain 3Re2-7. Moreover, we captured the transcriptome of P. brassicacearum 3Re2-7, confirming the transcription of the predicted biocontrol-related genes. The gene clusters coding for 2,4-diacetylphloroglucinol (phlABCDEFGH) and hydrogen cyanide (hcnABC) were shown to be highly transcribed. Further genes predicted to encode putative alginate production enzymes, a pyrroloquinoline quinone precursor peptide PqqA and a matrixin family metalloprotease were also found to be highly transcribed. With this study, we provide a basis to further characterize the mechanisms for biocontrol in Pseudomonas species, towards a sustainable and safe application of P. brassicacearum biocontrol agents.

", "keywords": ["COMPARATIVE GENOMICS", "0301 basic medicine", "570", "Antifungal Agents", "Plant-growth promotion", "Biolog\u00eda", "comparative genomics", "Phloroglucinol", "PLANT-GROWTH PROMOTION", "Article", "Rhizoctonia", "12. Responsible consumption", "transcriptomics", "03 medical and health sciences", "https://purl.org/becyt/ford/1.6", "Genome mining", "Hydrogen Cyanide", "Pseudomonas", "genome mining", "RNA SEQUENCING", "TRANSCRIPTOMICS", "biocontrol", "GENOME MINING", "PSEUDOMONASBRASSICACEARUM", "https://purl.org/becyt/ford/1", "Transcriptomics", "2. Zero hunger", "0303 health sciences", "Comparative genomics", "Biocontrol", "RNA sequencing", "Pseudomonas brassicacearum", "3. Good health", "BIOCONTROL", "Pseudomonas brassicacearum", "Biological Control Agents", "Genes", " Bacterial", "Transcriptome", "plant-growth promotion"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://doi.org/11336/151981"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11336/151981", "name": "item", "description": "11336/151981", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11336/151981"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-09T00:00:00Z"}}, {"id": "11336/226991", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-21T16:22:16Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "Abstract

Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.