Advancements in microbially explicit ecosystem models incorporate increasingly accurate representations of microbial physiology and enzyme\uffe2\uff80\uff90mediated depolymerization of soil organic matter in predicting biogeochemical responses to global change. However, a major challenge with model structural improvements is the requirement for additional parameters, which are often poorly constrained sources of uncertainty. Furthermore, it is often unclear how to best focus data collection efforts toward reducing model uncertainty. Here, we use Dual Arrhenius Michaelis\uffe2\uff80\uff90Menten Microbial Carbon and Nitrogen Physiology, a microbially mediated, coupled soil C and N cycling model, as a tool to explore the influence of microbial physiological and enzyme kinetic parameters on model estimates. We first quantify the potential for constraining model parameters using empirical measurements of soil respiration. We then use simulated data to identify which additional sources of data collection from the field would provide the greatest impact for constraining model estimates. We find that modeled soil C and N pools and fluxes are disproportionately sensitive to only a few parameters (e.g., activation energies and microbial CUE), while others exert less influence (e.g., Michaelis\uffe2\uff80\uff90Menten half\uffe2\uff80\uff90saturation constants). While some parameters can be constrained by the available data on heterotrophic respiration, the collection of additional data on dissolved organic C and N pools in the soil is identified as a high\uffe2\uff80\uff90priority data need. Improving our ability to model the interactions of soil microbial physiology, soil chemistry, enzyme activities, and environmental factors on C and N cycling will require closely considering model uncertainties and prioritizing future data collection opportunities based on their impact on model performance.Advancements in microbially explicit ecosystem models incorporate increasingly accurate representations of microbial physiology and enzyme\uffe2\uff80\uff90mediated depolymerization of soil organic matter in predicting biogeochemical responses to global change. However, a major challenge with model structural improvements is the requirement for additional parameters, which are often poorly constrained sources of uncertainty. Furthermore, it is often unclear how to best focus data collection efforts toward reducing model uncertainty. Here, we use Dual Arrhenius Michaelis\uffe2\uff80\uff90Menten Microbial Carbon and Nitrogen Physiology, a microbially mediated, coupled soil C and N cycling model, as a tool to explore the influence of microbial physiological and enzyme kinetic parameters on model estimates. We first quantify the potential for constraining model parameters using empirical measurements of soil respiration. We then use simulated data to identify which additional sources of data collection from the field would provide the greatest impact for constraining model estimates. We find that modeled soil C and N pools and fluxes are disproportionately sensitive to only a few parameters (e.g., activation energies and microbial CUE), while others exert less influence (e.g., Michaelis\uffe2\uff80\uff90Menten half\uffe2\uff80\uff90saturation constants). While some parameters can be constrained by the available data on heterotrophic respiration, the collection of additional data on dissolved organic C and N pools in the soil is identified as a high\uffe2\uff80\uff90priority data need. Improving our ability to model the interactions of soil microbial physiology, soil chemistry, enzyme activities, and environmental factors on C and N cycling will require closely considering model uncertainties and prioritizing future data collection opportunities based on their impact on model performance.Arctic and alpine tundra ecosystems are large reservoirs of organic carbon1,2. Climate warming may stimulate ecosystem respiration and release carbon into the atmosphere3,4. The magnitude and persistency of this stimulation and the environmental mechanisms that drive its variation remain uncertain5\uffe2\uff80\uff937. This hampers the accuracy of global land carbon\uffe2\uff80\uff93climate feedback projections7,8. Here we synthesize 136 datasets from 56 open-top chamber in situ warming experiments located at 28 arctic and alpine tundra sites which have been running for less than 1\uffe2\uff80\uff89year up to 25\uffe2\uff80\uff89years. We show that a mean rise of 1.4\uffe2\uff80\uff89\uffc2\uffb0C [confidence interval (CI) 0.9\uffe2\uff80\uff932.0\uffe2\uff80\uff89\uffc2\uffb0C] in air and 0.4\uffe2\uff80\uff89\uffc2\uffb0C [CI 0.2\uffe2\uff80\uff930.7\uffe2\uff80\uff89\uffc2\uffb0C] in soil temperature results in an increase in growing season ecosystem respiration by 30% [CI 22\uffe2\uff80\uff9338%] (n\uffe2\uff80\uff89=\uffe2\uff80\uff89136). Our findings indicate that the stimulation of ecosystem respiration was due to increases in both plant-related and microbial respiration (n\uffe2\uff80\uff89=\uffe2\uff80\uff899) and continued for at least 25\uffe2\uff80\uff89years (n\uffe2\uff80\uff89=\uffe2\uff80\uff89136). The magnitude of the warming effects on respiration was driven by variation in warming-induced changes in local soil conditions, that is, changes in total nitrogen concentration and pH and by context-dependent spatial variation in these conditions, in particular total nitrogen concentration and the carbon:nitrogen ratio. Tundra sites with stronger nitrogen limitations and sites in which warming had stimulated plant and microbial nutrient turnover seemed particularly sensitive in their respiration response to warming. The results highlight the importance of local soil conditions and warming-induced changes therein for future climatic impacts on respiration.50\u2009years of soil warming. Warming of >50\u2009years drove the ecosystem to a new steady state possessing a distinct biotic composition and reduced species richness, biomass and soil organic matter. However, the warmed state was preceded by an overreaction to warming, which was related to organism physiology and was evident after 5-8\u2009years. Ignoring this overreaction yielded errors of >100% for 83\u2009variables when predicting their responses to a realistic warming scenario of 1\u2009\u00b0C over 50\u2009years, although some, including soil carbon content, remained stable after 5-8\u2009years. This study challenges long-term ecosystem predictions made from short-term observations, and provides a framework for characterization of ecosystem responses to sustained climate change.", "keywords": ["0301 basic medicine", "570", "Environmental management", "INCREASES", "Ecosystem ecology", "Climate Change", "Evolutionary biology", "TERM", "630", "Article", "Carbon Cycle", "Soil", "03 medical and health sciences", "SDG 13 - Climate Action", "106026 Ecosystem research", "Life Below Water", "Ecosystem", "106022 Mikrobiologie", "0303 health sciences", "Ecology", "Climate-change ecology", "SHIFTS", "Biological Sciences", "15. Life on land", "Grassland", "106026 \u00d6kosystemforschung", "13. Climate action", "SDG 13 \u2013 Ma\u00dfnahmen zum Klimaschutz", "FEEDBACKS", "106022 Microbiology", "VEGETATION", "SENSITIVITY", "Environmental Sciences", "SOIL RESPIRATION", "RESPONSES"]}, "links": [{"href": "https://escholarship.org/content/qt99v0g8pc/qt99v0g8pc.pdf"}, {"href": "https://doi.org/10.1038/s41559-019-1055-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Ecology%20%26amp%3B%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41559-019-1055-3", "name": "item", "description": "10.1038/s41559-019-1055-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41559-019-1055-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-09T00:00:00Z"}}, {"id": "10.1038/s41558-023-01868-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:24Z", "type": "Journal Article", "created": "2023-12-04", "title": "The soil microbiome governs the response of microbial respiration to warming across the globe", "description": "Open AccessThe sensitivity of soil microbial respiration to warming (Q10) remains a major source of uncertainty surrounding the projections of soil carbon emissions to the atmosphere as the factors driving Q10 patterns across ecosystems have been assessed in isolation from each other. Here we report the results of a warming experiment using soils from 332 sites across all continents and major biomes to simultaneously evaluate the main drivers of global Q10 patterns. Compared with biochemical recalcitrance, mineral protection, substrate quantity and environmental factors, the soil microbiome (that is, microbial biomass and bacterial taxa) explained the largest portion of variation in Q10 values. Our work provides solid evidence that soil microbiomes largely govern the responses of soil heterotrophic respiration to warming and thus need to be explicitly accounted for when assessing land carbon\u2013climate feedbacks.", "keywords": ["2. Zero hunger", "Soil microbiome", "Microbial respiration", "13. Climate action", "XXXXXX - Unknown", "Warming", "15. Life on land", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.1038/s41558-023-01868-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Climate%20Change", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41558-023-01868-1", "name": "item", "description": "10.1038/s41558-023-01868-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41558-023-01868-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1038/srep34786", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:30Z", "type": "Journal Article", "created": "2016-10-10", "title": "Contrasting Effects Of Nitrogen And Phosphorus Addition On Soil Respiration In An Alpine Grassland On The Qinghai-Tibetan Plateau", "description": "Abstract
High soil organic carbon content, extensive root biomass, and low nutrient availability make alpine grasslands an important ecosystem for assessing the influence of nutrient enrichment on soil respiration (SR). We conducted a four-year (2009\uffe2\uff80\uff932012) field experiment in an alpine grassland on the Qinghai-Tibetan Plateau to examine the individual and combined effects of nitrogen (N, 100\uffe2\uff80\uff89kg ha\uffe2\uff88\uff921year\uffe2\uff88\uff921) and phosphorus (P, 50\uffe2\uff80\uff89kg ha\uffe2\uff88\uff921year\uffe2\uff88\uff921) addition on SR. We found that both N and P addition did not affect the overall growing-season SR but effects varied by year: with N addition SR increased in the first year but decreased during the last two years. However, while P addition did not affect SR during the first two years, SR increased during the last two years. No interactive effects of N and P addition were observed, and both N addition and P addition reduced heterotrophic respiration during the last year of the experiment. N and P addition affected SR via different processes: N mainly affected heterotrophic respiration, whereas P largely influenced autotrophic respiration. Our results highlight the divergent effects of N and P addition on SR and address the important potential of P enrichment for regulating SR and the carbon balance in alpine grasslands.
", "keywords": ["Biomass (ecology)", "0106 biological sciences", "Mechanics and Transport in Unsaturated Soils", "Nitrogen", "Soil Science", "Organic chemistry", "Plant Science", "Thermal Effects on Soil", "01 natural sciences", "Article", "Environmental science", "Agricultural and Biological Sciences", "Engineering", "Soil water", "Genetics", "Biology", "Ecosystem", "Civil and Structural Engineering", "2. Zero hunger", "Soil Fertility", "Ecology", "Bacteria", "Respiration", "Botany", "Life Sciences", "Plant Nutrient Uptake and Signaling Pathways", "Phosphorus", "Soil respiration", "04 agricultural and veterinary sciences", "15. Life on land", "Grassland", "Soil carbon", "Agronomy", "Chemistry", "13. Climate action", "FOS: Biological sciences", "Physical Sciences", "Heterotroph", "Growing season", "0401 agriculture", " forestry", " and fisheries", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "Animal science", "Nutrient"]}, "links": [{"href": "https://doi.org/10.1038/srep34786"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/srep34786", "name": "item", "description": "10.1038/srep34786", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/srep34786"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-10-10T00:00:00Z"}}, {"id": "10.1046/j.1365-2486.2001.00388.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:34Z", "type": "Journal Article", "created": "2003-03-11", "title": "Chemistry And Decomposition Of Litter From Populus Tremuloides Michaux Grown At Elevated Atmospheric Co2 And Varying N Availability", "description": "SummaryIt has been hypothesized that greater production of total nonstructural carbohydrates (TNC) in foliage grown under elevated atmospheric carbon dioxide (CO2) will result in higher concentrations of defensive compounds in tree leaf litter, possibly leading to reduced rates of decomposition and nutrient cycling in forest ecosystems of the future. To evaluate the effects of elevated atmospheric CO2on litter chemistry and decomposition, we performed a 111 day laboratory incubation with leaf litter of trembling aspen (Populus tremuloidesMichaux) produced at 36\uffe2\uff80\uff83Pa and 56\uffe2\uff80\uff83Pa CO2and two levels of soil nitrogen (N) availability. Decomposition was quantified as microbially respired CO2and dissolved organic carbon (DOC) in soil solution, and concentrations of nonstructural carbohydrates, N, carbon (C), and condensed tannins were monitored throughout the incubation. Growth under elevated atmospheric CO2did not significantly affect initial litter concentrations of TNC, N, or condensed tannins. Rates of decomposition, measured as both microbially respired CO2and DOC did not differ between litter produced under ambient and elevated CO2. Total C lost from the samples was 38\uffe2\uff80\uff83mg\uffe2\uff80\uff83g\uffe2\uff88\uff921litter as respired CO2and 138\uffe2\uff80\uff83mg\uffe2\uff80\uff83g\uffe2\uff88\uff921litter as DOC, suggesting short\uffe2\uff80\uff90term pulses of dissolved C in soil solution are important components of the terrestrial C cycle. We conclude that litter chemistry and decomposition in trembling aspen are minimally affected by growth under higher concentrations of CO2.
", "keywords": ["Ecology and Evolutionary Biology", "carbohydrates", "Quaking aspen", "forest-soil", "litter-plant", "nitrogen", "nitrogen-", "Microlysimeter", "soil-chemistry", "cycling-", "populus-tremuloides", "Geology and Earth Sciences", "Soil Carbon", "Microbiology of soils", "Carbon cycle", "04 agricultural and veterinary sciences", "GLOBAL-ECOLOGY", "chemical-composition", "Organic-matter", "soil-solution", "nutrient-availability", "Tannin", "leaf-litter", "Science", "decomposition-", "Nutrient enrichment", "Carbohydrates", "carbohydrates-", "respiration-", "carbon-dioxide-enrichment", "Nitrogen in soil", "michigan-", "carbon sinks", "C", "Nutrient budget of forests", "Litter", "Populus tremuloides", "Global Change", "tannins-", "Decomposition", "forest-litter", "Foliage", "Carbon dioxide effects on forest litter", "Climatic changes", "15. Life on land", "carbon-nitrogen-ratio", "Forest litter decomposition", "N Ratio", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "microbial-activities", "nitrogen-content"]}, "links": [{"href": "https://doi.org/10.1046/j.1365-2486.2001.00388.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1046/j.1365-2486.2001.00388.x", "name": "item", "description": "10.1046/j.1365-2486.2001.00388.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1046/j.1365-2486.2001.00388.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2001-01-01T00:00:00Z"}}, {"id": "10.1046/j.1365-2486.1999.00211.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:34Z", "type": "Journal Article", "created": "2003-03-11", "title": "Elevated Co2 And Temperature Impacts On Different Components Of Soil Co2 Efflux In Douglas-Fir Terracosms", "description": "AbstractAlthough numerous studies indicate that increasing atmospheric CO2 or temperature stimulate soil CO2 efflux, few data are available on the responses of three major components of soil respiration [i.e. rhizosphere respiration (root and root exudates), litter decomposition, and oxidation of soil organic matter] to different CO2 and temperature conditions. In this study, we applied a dual stable isotope approach to investigate the impact of elevated CO2 and elevated temperature on these components of soil CO2 efflux in Douglas\uffe2\uff80\uff90fir terracosms. We measured both soil CO2 efflux rates and the 13C and 18O isotopic compositions of soil CO2 efflux in 12 sun\uffe2\uff80\uff90lit and environmentally controlled terracosms with 4\uffe2\uff80\uff90year\uffe2\uff80\uff90old Douglas fir seedlings and reconstructed forest soils under two CO2 concentrations (ambient and 200 ppmv above ambient) and two air temperature regimes (ambient and 4 \uffc2\uffb0C above ambient). The stable isotope data were used to estimate the relative contributions of different components to the overall soil CO2 efflux. In most cases, litter decomposition was the dominant component of soil CO2 efflux in this system, followed by rhizosphere respiration and soil organic matter oxidation. Both elevated atmospheric CO2 concentration and elevated temperature stimulated rhizosphere respiration and litter decomposition. The oxidation of soil organic matter was stimulated only by increasing temperature. Release of newly fixed carbon as root respiration was the most responsive to elevated CO2, while soil organic matter decomposition was most responsive to increasing temperature. Although some assumptions associated with this new method need to be further validated, application of this dual\uffe2\uff80\uff90isotope approach can provide new insights into the responses of soil carbon dynamics in forest ecosystems to future climate changes.
", "keywords": ["elevated CO2", "13. Climate action", "stable isotopes", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "forest ecosystem", "15. Life on land", "global warming", "soil respiration"], "contacts": [{"organization": "Guanghui Lin, Guanghui Lin, Mark Johnson, David T. Tingey, James R. Ehleringer, Paul T. Rygiewicz,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1046/j.1365-2486.1999.00211.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1046/j.1365-2486.1999.00211.x", "name": "item", "description": "10.1046/j.1365-2486.1999.00211.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1046/j.1365-2486.1999.00211.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1999-02-01T00:00:00Z"}}, {"id": "10.1046/j.1469-8137.2003.00911.x", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:18:37Z", "type": "Journal Article", "created": "2003-11-17", "title": "Fine-Root Respiration In A Loblolly Pine And Sweetgum Forest Growing In Elevated Co2", "description": "\u2022\u2002 The loss of carbon below-ground through respiration of fine roots may be modified by global change. Here we tested the hypothesis that a reduction in N concentration of tree fine-roots grown in an elevated atmospheric CO2 concentration would reduce maintenance respiration and that more energy would be used for root growth and N uptake. We partitioned total fine-root respiration (RT ) between maintenance (RM ), growth (RG ), and N uptake respiration (RN ) for loblolly pine (Pinus taeda) and sweetgum (Liquidambar styraciflua) forests exposed to elevated CO2 . \u2022\u2002 A substantial increase in fine-root production contributed to a 151% increase in RG for loblolly pine in elevated CO2 . Root specific RM for pine was 24% lower under elevated CO2 but when extrapolated to the entire forest, no treatment effect could be detected. \u2022\u2002 R G (<\u00a010%) and RN (<\u00a03%) were small components of RM in both forests. Maintenance respiration was the vast majority of RT , and contributed 92% and 86% of these totals at the pine and sweetgum forests, respectively. \u2022\u2002 The hypothesis was rejected because the majority of fine-root respiration was used for maintenance and was not reduced by changes in root N concentration in elevated CO2 . Because of its large contribution to RT and total soil CO2 efflux, changes in RM caused by warming may greatly alter carbon losses from forests to the atmosphere.", "keywords": ["0106 biological sciences", "Temperate forest", "Sweetgum (Liquidambar styeaciflua)", "Growth respiration", "Loblolly pine (Pinus taeda)", "Maintenance respiration", "Nitrogen uptake respiration", "15. Life on land", "Free-air CO enrichment (FACE) 2", "01 natural sciences", "Annual fine-root respiration"]}, "links": [{"href": "https://doi.org/10.1046/j.1469-8137.2003.00911.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1046/j.1469-8137.2003.00911.x", "name": "item", "description": "10.1046/j.1469-8137.2003.00911.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1046/j.1469-8137.2003.00911.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-11-17T00:00:00Z"}}, {"id": "10.1093/femsec/fiae152", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:03Z", "type": "Journal Article", "created": "2024-11-19", "title": "A respiro-fermentative strategy to survive nanoxia in Acidobacterium capsulatum", "description": "AbstractMicrobial soil habitats are characterized by rapid shifts in substrate and nutrient availabilities, as well as chemical and physical parameters. One such parameter that can vary in soil is oxygen; thus, microbial survival is dependent on adaptation to this substrate. To better understand the metabolic abilities and adaptive strategies to oxygen-deprived environments, we combined genomics with transcriptomics of a model organism, Acidobacterium capsulatum, to explore the effect of decreasing, environmentally relevant oxygen concentrations. The decrease from 10 to 0.1\uffc2\uffa0\uffc2\uffb5M oxygen (3.6 to 0.036 pO2% present atmospheric level, respectively) caused the upregulation of the transcription of genes involved in signal transduction mechanisms, energy production and conversion and secondary metabolites biosynthesis, transport, and catabolism based on clusters of orthologous group categories. Contrary to established observations for aerobic metabolism, key genes in oxidative stress response were significantly upregulated at lower oxygen concentrations, presumably due to an NADH/NAD+ redox ratio imbalance as the cells transitioned into nanoxia. Furthermore, A. capsulatum adapted to nanoxia by inducing a respiro-fermentative metabolism and rerouting fluxes of its central carbon and energy pathways to adapt to high NADH/NAD+ redox ratios. Our results reveal physiological features and metabolic capabilities that allowed A. capsulatum to adapt to oxygen-limited conditions, which could expand into other environmentally relevant soil strains.Microplastic pollution is a topic of increasing concern, especially since this issue was first addressed in soils. Results have so far been variable in terms of effects, suggesting that there is substantial context-dependency in microplastic effects in soil. To better define conditions that may affect microplastic-related impacts, we here examined effects as a function of microplastic shape and polymer type, and we tested if effects on soil properties and soil microbial activities change with incubation time. In our laboratory study, we evaluated twelve different secondary microplastics representing four microplastic shapes: fibers, films, foams and fragments; and eight polymer types: polyamide (PA), polycarbonate (PC), polyethylene (PE), polyester (PES), polyethylene terephthalate (PET), polypropylene (PP), polystyrene (PS), and polyurethane (PU). We mixed the microplastics with a sandy soil (0.4% w/w) and incubated at 25\uffc2\uffb0C for 31\uffc2\uffa0days. Then, we collected soil samples on the 3rd, 11th, and 31st\uffc2\uffa0day, and measured soil pH, respiration and four enzyme activities (soil enzymatic activities). Our results showed that microplastics could affect soil pH, respiration and enzymatic activities depending on microplastic shape and polymer type, effects that were altered with incubation time. Soil pH increased with foams and fragments and overall decreased in the first days of incubation and then increased. Soil respiration increased with PE foams and was affected by the incubation time, declining over time. Overall, acid phosphatase activity was not affected by shape or polymer type. \uffce\uffb2-D-glucosidase activity decreased with foams, cellobiosidase activity decreased with fibers, films and foams while N-acetyl-\uffce\uffb2-glucosaminidase activities decreased with fibers and fragments. Enzymatic activities fluctuated during the incubation time, except N-acetyl-\uffce\uffb2-glucosaminidase, which showed a declining trend with incubation time. Enzymatic activities were negatively correlated with soil pH and this relationship was less strong when microplastics were added to the soil. Our study adds to the evidence that research should embrace the complexity and diversity of microplastics, highlighting the role of microplastic shape and polymer type in influencing effects; additionally, we show that incubation time is also a parameter to consider, as effects are dynamic even in the short term.
", "keywords": ["580", "2. Zero hunger", "pH", "foams", "04 agricultural and veterinary sciences", "fibers", "15. Life on land", "soil respiration", "01 natural sciences", "6. Clean water", "Environmental sciences", "soil enzymatic activities", "500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::580 Pflanzen (Botanik)", "13. Climate action", "fragments", "0401 agriculture", " forestry", " and fisheries", "GE1-350", "films", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.3389/fenvs.2021.675803"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fenvs.2021.675803", "name": "item", "description": "10.3389/fenvs.2021.675803", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fenvs.2021.675803"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-23T00:00:00Z"}}, {"id": "10.3389/fnut.2016.00019", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:33Z", "type": "Journal Article", "created": "2016-06-16", "title": "Drivers Of Plant-Availability Of Phosphorous From Thermally Conditioned Sewage Sludge As Assessed By Isotopic Labeling", "description": "Urban sewage sludge is a potential source of phosphorus (P) for agriculture and represents an alternative way to recycle P as fertilizer. However, the use of thermally conditioned sewage sludge (TCSS) required an accurate assessment of its value as P-fertilizer. This work aimed at assessing the plant-availability of P from TCSS. Uptake of P by a mixture of ryegrass and fescue from TCSS and triple super phosphate (TSP) fertilizers was studied using (32)P-labeling technique in a greenhouse experiment. Phosphorus was applied at the rate of 50\u2009mg P kg(-1).We also conducted incubation experiments considering the same treatments to assess soil microbial respiration. Applications of TCSS and TSP increased plant P uptake that is related to the root P acquisition. The P taken up by plant from soil plant-available P was lower for control compared to TSP or TCSS that was attributed to the increase of root interception of soil P. The contribution of TSP to ryegrass nutrition (Pdff%) was 55% with 22% of the applied P which was taken up by plants (CPU%). The Pdff value for TCSS was 56% with 14% of fertilizer P recovery (CPU%). Shoot biomass and total P uptake from TCSS were lower than those from TSP. As a result, the agronomic effectiveness of TCSS calculated from Pdff value (in comparison with TSP treatment) was 102%, while the AE of TCSS estimated from CPU value (in % TSP) was 64%, which is attributed to microbial activity stimulation inducing P immobilization onto soil constituents and microbial biomass during plant growth. The high C/N ratio of TCSS stimulated soil microbial biomass that competes with plant roots to acquire nutrients, such as P. As a consequence, the P taken up from either native soil or TCSS decreased in similar proportions. The AE value calculated with Pdff% took into account these interactions between soil, plant, and microbial biomass, and is less dependent on operational conditions than the AE value calculated with %Precovery.", "keywords": ["[SDV.SA]Life Sciences [q-bio]/Agricultural sciences", "ryegrass", "recyclage des d\u00e9chets", "microbial P", "soil respiration", "7. Clean energy", "12. Responsible consumption", "ray-grass", "f\u00e9tuque", "waste recycling", "disponibilit\u00e9 en phosphore", "Nutrition", "580", "2. Zero hunger", "P immobilization", "[SDV.SA] Life Sciences [q-bio]/Agricultural sciences", "traitement des boues", "sewage sludge", "04 agricultural and veterinary sciences", "15. Life on land", "fertilizer", "6. Clean water", "plant-available P", "engrais", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "32P-labeling technique"]}, "links": [{"href": "https://doi.org/10.3389/fnut.2016.00019"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fnut.2016.00019", "name": "item", "description": "10.3389/fnut.2016.00019", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fnut.2016.00019"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-06-16T00:00:00Z"}}, {"id": "10.1093/femsle/fnab100", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:03Z", "type": "Journal Article", "created": "2021-07-30", "title": "Transcriptomic markers of fungal growth, respiration and carbon-use efficiency", "description": "ABSTRACTFungal metabolic carbon acquisition and its subsequent partitioning between biomass production and respiration, i.e. the carbon-use efficiency (CUE), are central parameters in biogeochemical modeling. However, current available techniques for estimating these parameters are all associated with practical and theoretical shortcomings, making assessments unreliable. Gene expression analyses hold the prospect of phenotype prediction by indirect means, providing new opportunities to obtain information about metabolic priorities. We cultured four different fungal isolates (Chalara longipes, Laccaria bicolor, Serpula lacrymans and Trichoderma harzianum) in liquid media with contrasting nitrogen availability and measured growth rates and respiration to calculate CUE. By relating gene expression markers to measured carbon fluxes, we identified genes coding for 1,3-\uffce\uffb2-glucan synthase and 2-oxoglutarate dehydrogenase as suitable markers for growth and respiration, respectively, capturing both intraspecific variation as well as within-strain variation dependent on growth medium. A transcript index based on these markers correlated significantly with differences in CUE between the fungal isolates. Our study paves the way for the use of these markers to assess differences in growth, respiration and CUE in natural fungal communities, using metatranscriptomic or the RT-qPCR approach.With continuous nitrogen (N) enrichment and sulfur (S) deposition, soil acidification has accelerated and become a global environmental issue. However, a full understanding of the general pattern of ecosystem belowground processes in response to soil acidification due to the impacting factors remains elusive. We conducted a meta-analysis of soil acidification impacts on belowground functions using 304 observations from 49 independent studies, mainly including soil cations, soil nutrient, respiration, root and microbial biomass. Our results show that acid addition significantly reduced soil pH by 0.24 on average, with less pH decrease in forest than non-forest ecosystems. The response ratio of soil pH was positively correlated with site precipitation and temperature, but negatively with initial soil pH. Soil base cations (Ca2+, Mg2+, Na+) decreased while non-base cations (Al3+, Fe3+) increased with soil acidification. Soil respiration, fine root biomass, microbial biomass carbon and nitrogen were significantly reduced by 14.7%, 19.1%, 9.6% and 12.1%, respectively, under acid addition. These indicate that soil carbon processes are sensitive to soil acidification. Overall, our meta-analysis suggests a strong negative impact of soil acidification on belowground functions, with the potential to suppress soil carbon emission. It also arouses our attention to the toxic effects of soil ions on terrestrial ecosystems.
", "keywords": ["Biomass (ecology)", "Organic chemistry", "Soil pH", "soil respiration", "Environmental technology. Sanitary engineering", "Agricultural and Biological Sciences", "Engineering", "Terrestrial ecosystem", "Soil water", "Climate change", "GE1-350", "TD1-1066", "Ecology", "Physics", "Soil Water Retention", "Ocean acidification", "Q", "Life Sciences", "Soil respiration", "04 agricultural and veterinary sciences", "Soil carbon", "6. Clean water", "Chemistry", "Physical Sciences", "Environmental chemistry", "soil cations", "microbes", "Mechanics and Transport in Unsaturated Soils", "Nitrogen", "Science", "QC1-999", "Materials Science", "Soil Science", "Thermal Effects on Soil", "Environmental science", "Biomaterials", "soil pH", "acid deposition", "Soil Carbon Sequestration", "Biology", "Soil acidification", "Ecosystem", "Civil and Structural Engineering", "Applications of Clay Nanotubes in Various Fields", "Soil science", "Soil organic matter", "Soil Fertility", "15. Life on land", "Soil biodiversity", "Agronomy", "meta-analysis", "Environmental sciences", "Soil Hydraulic Properties", "13. Climate action", "FOS: Biological sciences", "Bulk soil", "0401 agriculture", " forestry", " and fisheries", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "Nutrient"]}, "links": [{"href": "https://doi.org/10.1088/1748-9326/ab239c"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1088/1748-9326/ab239c", "name": "item", "description": "10.1088/1748-9326/ab239c", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1088/1748-9326/ab239c"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-07-01T00:00:00Z"}}, {"id": "10.1093/ismejo/wrae025", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:04Z", "type": "Journal Article", "created": "2024-02-12", "title": "Stronger compensatory thermal adaptation of soil microbial respiration with higher substrate availability", "description": "AbstractOngoing global warming is expected to augment soil respiration by increasing the microbial activity, driving self-reinforcing feedback to climate change. However, the compensatory thermal adaptation of soil microorganisms and substrate depletion may weaken the effects of rising temperature on soil respiration. To test this hypothesis, we collected soils along a large-scale forest transect in eastern China spanning a natural temperature gradient, and we incubated the soils at different temperatures with or without substrate addition. We combined the exponential thermal response function and a data-driven model to study the interaction effect of thermal adaptation and substrate availability on microbial respiration and compared our results to those from two additional continental and global independent datasets. Modeled results suggested that the effect of thermal adaptation on microbial respiration was greater in areas with higher mean annual temperatures, which is consistent with the compensatory response to warming. In addition, the effect of thermal adaptation on microbial respiration was greater under substrate addition than under substrate depletion, which was also true for the independent datasets reanalyzed using our approach. Our results indicate that thermal adaptation in warmer regions could exert a more pronounced negative impact on microbial respiration when the substrate availability is abundant. These findings improve the body of knowledge on how substrate availability influences the soil microbial community\uffe2\uff80\uff93temperature interactions, which could improve estimates of projected soil carbon losses to the atmosphere through respiration.Excess water can induce flooding stress resulting in yield loss, even in wetland crops such as rice (Oryza). However, traits from species of wild Oryza have already been used to improve tolerance to abiotic stress in cultivated rice. This study aimed to establish root responses to sudden soil flooding among eight wild relatives of rice with different habitat preferences benchmarked against three genotypes of O. sativa. Plants were raised hydroponically, mimicking drained or flooded soils, to assess the plasticity of adventitious roots. Traits included were apparent permeance (PA) to O2 of the outer part of the roots, radial water loss, tissue porosity, apoplastic barriers in the exodermis, and root anatomical traits. These were analysed using a plasticity index and hierarchical clustering based on principal component analysis. For example, O. brachyantha, a wetland species, possessed very low tissue porosity compared with other wetland species, whereas dryland species O. latifolia and O. granulata exhibited significantly lower plasticity compared with wetland species and clustered in their own group. Most species clustered according to growing conditions based on PA, radial water loss, root porosity, and key anatomical traits, indicating strong anatomical and physiological responses to sudden soil flooding.Carbon (C) assimilation can be severely impaired during periods of environmental stress, like drought or defoliation, making trees heavily dependent on the use of C reserve pools for survival; yet, the dynamics of reserve use during periods of reduced C supply are still poorly understood. We used stem girdling in mature poplar trees (Populus tremula L. hybrids), a lipid-storing species, to permanently interrupt the phloem C transport and induced C shortage in the isolated stem section below the girdle and monitored metabolic activity during three campaigns in the growing seasons of 2018, 2019 and 2021. We measured respiratory fluxes (CO2 and O2), non-structural carbon concentration, the respiratory substrate (based on isotopic analysis and CO2/O2 ratio) and the age of the respiratory substrate (based on radiocarbon analysis). Our study shows that poplar trees can survive long periods of reduced C supply from the canopy by switching in metabolism from recent carbohydrates to older storage pools with a potential mixture of respiratory substrates, including lipids. This mechanism of stress resilience can explain why tree decline may take many years before death occurs.
Drought periods are projected to become more severe and more frequent in many European regions. While effects of single strong droughts on plant and microbial carbon (C) dynamics have been studied in some detail, impacts of recurrent drought events are still little understood.
We tested whether the legacy of extreme experimental drought affects responses of plant and microbial C and nitrogen (N) turnover to further drought and rewetting. In a mountain grassland, we conducted a 13C pulse\uffe2\uff80\uff90chase experiment during a naturally occurring drought and rewetting event in plots previously exposed to experimental droughts and in ambient controls (AC). After labelling, we traced 13C below\uffe2\uff80\uff90ground allocation and incorporation into soil microbes using phospholipid fatty acid biomarkers.
Drought history (DH) had no effects on the standing shoot and fine root plant biomass. However, plants with experimental DH displayed decreased shoot N concentrations and increased fine root N concentrations relative to those in AC. During the natural drought, plants with DH assimilated and allocated less 13C below\uffe2\uff80\uff90ground; moreover, fine root respiration was reduced and not fuelled by fresh C compared to plants in AC.
Regardless of DH, microbial biomass remained stable during natural drought and rewetting. Although microbial communities initially differed in their composition between soils with and without DH, they responded to the natural drought and rewetting in a similar way: gram\uffe2\uff80\uff90positive bacteria increased, while fungal and gram\uffe2\uff80\uff90negative bacteria remained stable. In soils with DH, a strongly reduced uptake of recent plant\uffe2\uff80\uff90derived 13C in microbial biomarkers was observed during the natural drought, pointing to a smaller fraction of active microbes or to a microbial community that is less dependent on plant C.
Synthesis. Drought history can induce changes in above\uffe2\uff80\uff90 vs. below\uffe2\uff80\uff90ground plant N concentrations and affect the response of plant C turnover to further droughts and rewetting by decreasing plant C uptake and below\uffe2\uff80\uff90ground allocation. DH does not affect the responses of the microbial community to further droughts and rewetting, but alters microbial functioning, particularly the turnover of recent plant\uffe2\uff80\uff90derived carbon, during and after further drought periods.
", "keywords": ["0301 basic medicine", "plant-soil (below-ground) interactions", "NITROGEN TURNOVER", "Biomass Allocation", "microbial community composition", "Negibacteria", "drought", "phospholipid fatty acid", "nitrogen", "Microbial community composition", "Plant\u2013Soil (Below\u2010ground) Interactions", "Recovery", "ROOT RESPIRATION", "Plant-soil (below-ground) interactions", "CLIMATE EXTREMES", "C pulse labelling", "Below-ground carbon allocation", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "SOIL INTERACTIONS", "below-ground carbon allocation", "C-13 pulse labelling", "Grassland", "6. Clean water", "Europe", "Phospholipid", "ORGANIC-MATTER", "Mountain Region", "Posibacteria", "DIOXIDE PULSES", "Phospholipid fatty acid", "106022 Microbiology", "Root/shoot Ratio", "Belowground Biomass", "Ecosystem Resilience", "Nitrogen", "Microbial Community", "Carbon Isotope", "Soil-vegetation Interaction", "recovery", "SUMMER DROUGHT", "03 medical and health sciences", "Rewetting", "Community Composition", "plant\u2013soil (below-ground) interactions", "WATER-STRESS", "resilience", "Drought", "Resilience", "RESILIENCE", "15. Life on land", "Turnover", "Microbial Activity", "13. Climate action", "Fatty Acid", "RESPONSES"]}, "links": [{"href": "https://doi.org/10.1111/1365-2745.12593"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1365-2745.12593", "name": "item", "description": "10.1111/1365-2745.12593", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1365-2745.12593"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-05-24T00:00:00Z"}}, {"id": "10.1111/geb.13371", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:29Z", "type": "Journal Article", "created": "2021-08-18", "title": "Large-scale drivers of relationships between soil microbial properties and organic carbon across Europe", "description": "AbstractAimQuantify direct and indirect relationships between soil microbial community properties (potential basal respiration, microbial biomass) and abiotic factors (soil, climate) in three major land\uffe2\uff80\uff90cover types.
LocationEurope.
Time period2018.
Major taxa studiedMicrobial community (fungi and bacteria).
MethodsWe collected 881 soil samples from across Europe in the framework of the Land Use/Land Cover Area Frame Survey (LUCAS). We measured potential soil basal respiration at 20\uffc2\uffa0\uffc2\uffbaC and microbial biomass (substrate\uffe2\uff80\uff90induced respiration) using an O2\uffe2\uff80\uff90microcompensation apparatus. Soil and climate data were obtained from the same LUCAS survey and online databases. Structural equation models (SEMs) were used to quantify relationships between variables, and equations extracted from SEMs were used to create predictive maps. Fatty acid methyl esters were measured in a subset of samples to distinguish fungal from bacterial biomass.
ResultsSoil microbial properties in croplands were more heavily affected by climate variables than those in forests. Potential soil basal respiration and microbial biomass were correlated in forests but decoupled in grasslands and croplands, where microbial biomass depended on soil carbon. Forests had a higher ratio of fungi to bacteria than grasslands or croplands.
Main conclusionsSoil microbial communities in grasslands and croplands are likely carbon\uffe2\uff80\uff90limited in comparison with those in forests, and forests have a higher dominance of fungi indicating differences in microbial community composition. Notably, the often already\uffe2\uff80\uff90degraded soils of croplands could be more vulnerable to climate change than more natural soils. The provided maps show potentially vulnerable areas that should be explicitly accounted for in future management plans to protect soil carbon and slow the increasing vulnerability of European soils to climate change.
", "keywords": ["2. Zero hunger", "570", "Land cover", "Take urgent action to combat climate change and its impacts", "Soil microbial biomass", "soil microbial respiration", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie", "04 agricultural and veterinary sciences", "structural equation modelling", "15. Life on land", "Soil carbon", "croplands", "soil microbial biomass", "Europe", "climate change", "land cover", "Structural equation modelling", "13. Climate action", "Climate change", "0401 agriculture", " forestry", " and fisheries", "http://metadata.un.org/sdg/13", "Croplands", "soil carbon", "Soil microbial respiration"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/geb.13371"}, {"href": "https://doi.org/10.1111/geb.13371"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Ecology%20and%20Biogeography", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/geb.13371", "name": "item", "description": "10.1111/geb.13371", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/geb.13371"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-18T00:00:00Z"}}, {"id": "10.1111/1365-2664.13839", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:18Z", "type": "Journal Article", "created": "2021-01-19", "title": "Effects of microplastics and drought on soil ecosystem functions and multifunctionality", "description": "Abstract
Microplastics in soils have become an important threat for terrestrial systems as they may potentially alter the geochemical/biophysical soil environment and can interact with drought. As microplastics may affect soil water content, this could exacerbate the well\uffe2\uff80\uff90known negative effects of drought on ecosystem functionality. Thus, functions including litter decomposition, soil aggregation or those related with nutrient cycling can be altered. Despite this potential interaction, we know relatively little about how microplastics, under different soil water conditions, affect ecosystem functions and multifunctionality.
To address this gap, we performed an experiment using grassland plant communities growing in microcosms. Microplastic fibres (absent, present) and soil water conditions (well\uffe2\uff80\uff90watered, drought) were applied in a fully factorial design. At harvest, we measured soil ecosystem functions related to nutrient cycling (\uffce\uffb2\uffe2\uff80\uff90glucosaminidase, \uffce\uffb2\uffe2\uff80\uff90D\uffe2\uff80\uff90cellobiosidase, phosphatase, \uffce\uffb2\uffe2\uff80\uff90glucosidase enzymes), respiration, nutrient retention, pH, litter decomposition and soil aggregation (water stable aggregates). As terrestrial systems provide these functions simultaneously, we also assessed ecosystem multifunctionality, an index that encompasses the array of ecosystem functions measured here.
We found that the interaction between microplastic fibres and drought affected ecosystem functions and multifunctionality. Drought had negatively affected nutrient cycling by decreasing enzymatic activities by up to ~39%, while microplastics increased soil aggregation by ~18%, soil pH by ~4% and nutrient retention by up to ~70% by diminishing nutrient leaching. Microplastic fibres also impacted soil enzymes, respiration and ecosystem multifunctionality, but importantly, the direction of these effects depended on soil water status. That is, under well\uffe2\uff80\uff90watered conditions, these functions decreased with microplastic fibres by up to ~34% while under drought they had similar values irrespective of the microplastic presence, or tended to increase with microplastics. Litter decomposition had a contrary pattern increasing with microplastics by ~6% under well\uffe2\uff80\uff90watered conditions while decreasing to a similar percentage under drought.
Synthesis and applications. Single ecosystem functions can be positively or negatively affected by microplastics fibres depending on soil water status. However, our results suggest that microplastic fibres may cause negative effects on ecosystem soil multifunctionality of a similar magnitude as drought. Thus, strategies to counteract this new global change factor are necessary.
", "keywords": ["2. Zero hunger", "570", "ddc:630", "nutrient cycling", "litter decomposition", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie", "04 agricultural and veterinary sciences", "15. Life on land", "soil respiration", "01 natural sciences", "6. Clean water", "soil aggregation", "soil pH", "grasslands ecosystem", "13. Climate action", "nutrient leaching", "0401 agriculture", " forestry", " and fisheries", "ddc:570", "Institut f\u00fcr Biochemie und Biologie", "enzymatic activities", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://besjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1365-2664.13839"}, {"href": "https://doi.org/10.1111/1365-2664.13839"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Applied%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1365-2664.13839", "name": "item", "description": "10.1111/1365-2664.13839", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1365-2664.13839"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-10T00:00:00Z"}}, {"id": "10.1111/ejss.12095", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:21Z", "type": "Journal Article", "created": "2013-10-12", "title": "Effect Of Biochar Addition On Soil Respiration Partitioning And Root Dynamics In An Apple Orchard", "description": "SummaryBiochar addition to soil has been suggested as a promising strategy to increase soil carbon storage with important side\uffe2\uff80\uff90effects on soil fertility and crop productivity. Understanding the effect of biochar on soil respiration partitioning into rhizosphere\uffe2\uff80\uff90derived (Fr) and soil organic carbon\uffe2\uff80\uff90derived (Fsoc) components and on plant root dynamics and microbial activity is a crucial issue in the prediction of the impact of biochar on soil organic carbon and nutrient cycles. Within this framework, an experiment was carried out in an apple (Malus domestica Bork) orchard located in the experimental farm of the Bologna University (Italy). In spring 2009, 10\uffe2\uff80\uff89t of biochar per hectare were incorporated into the surface 20\uffe2\uff80\uff90cm soil layer by soil ploughing. The trenching method was used in order to partition total soil respiration (Fs) into Fr and Fsoc components in both biochar\uffe2\uff80\uff90treated and control soil. Soil respiration measurements were performed from June 2009 to March 2011. To study root dynamics, polycarbonate boxes were built and buried into the soil. Soil profile pictures were collected fortnightly with a CCD sensor scanner inserted in the boxes and analysed with the WinRHIZO Tron MF software. Biochar addition increased Fsoc and reduced Fr, even if the root length intensity (La) increased in biochar\uffe2\uff80\uff90treated soils relative to that in the control. A decrease in root metabolic activity was postulated to explain these contrasting results.
", "keywords": ["2. Zero hunger", "biochar", " soil respiration", " root growth", " apple", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "3. Good health"]}, "links": [{"href": "https://doi.org/10.1111/ejss.12095"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/ejss.12095", "name": "item", "description": "10.1111/ejss.12095", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/ejss.12095"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-10-02T00:00:00Z"}}, {"id": "10.1111/ejss.13290", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:22Z", "type": "Journal Article", "created": "2022-07-25", "title": "Post\u2010fire recovery of soil microbial functions is promoted by plant growth", "description": "AbstractForest fires can alter the biological properties of soils. There is increasing evidence that fires cause a shift in soil microbial communities, which play a central role in forest carbon and nutrient cycling. In this study, we evaluate the effect of soil heating on soil microbial functions. We hypothesised that fire reduces the catabolic functional diversity of soil, and that post\uffe2\uff80\uff90fire plant growth enhances its recovery. To test this, we experimentally heated a forest soil at 200\uffc2\uffb0C (T200) or 450\uffc2\uffb0C (T450). Heated and unheated soils were then incubated in tubs with or without live grass (Lolium perenne L.). We determined the functional profiles by measuring the substrate\uffe2\uff80\uff90induced respiration (SIR) using the Microresp\uffe2\uff84\uffa2 technique and analysed nutrient availability at the end of the incubation. At both temperatures, soil heating altered the respiration responses to substrate additions and the catabolic functional diversity of soils. Functional diversity was initially reduced in T200 soils but recovered at the end of the incubation. In contrast, T450 soils initially maintained the catabolic functional diversity, but decreased at the end of the incubation. Heating\uffe2\uff80\uff90induced nutrient availability stimulated the growth of grass, which in turn increased the response to several substrates and increased the functional diversity to values similar to the unheated controls. Our results suggest that fire\uffe2\uff80\uff90driven alteration of soil microbial communities has consequences at a functional level, and that the recovery of plant communities enhances the recovery of soil microbial functions.
Highlights
Soil experimental heating altered microbial functions and reduced soil functional diversity.
Soil heating also increased nutrient availability, enhancing plant growth.
Growth of plants promoted the recovery of soil functional diversity.
Post\uffe2\uff80\uff90fire recovery of functional diversity may be related to the recovery of photosynthetic tissues.
Rising levels of atmospheric CO2 frequently stimulate plant inputs to soil, but the consequences of these changes for soil carbon (C) dynamics are poorly understood. Plant\uffe2\uff80\uff90derived inputs can accumulate in the soil and become part of the soil C pool (\uffe2\uff80\uff9cnew soil C\uffe2\uff80\uff9d), or accelerate losses of pre\uffe2\uff80\uff90existing (\uffe2\uff80\uff9cold\uffe2\uff80\uff9d) soil C. The dynamics of the new and old pools will likely differ and alter the long\uffe2\uff80\uff90term fate of soil C, but these separate pools, which can be distinguished through isotopic labeling, have not been considered in past syntheses. Using meta\uffe2\uff80\uff90analysis, we found that while elevated CO2 (ranging from 550 to 800 parts per million by volume) stimulates the accumulation of new soil C in the short term (<1\uffc2\uffa0year), these effects do not persist in the longer term (1\uffe2\uff80\uff934\uffc2\uffa0years). Elevated CO2 does not affect the decomposition or the size of the old soil C pool over either temporal scale. Our results are inconsistent with predictions of conventional soil C models and suggest that elevated CO2 might increase turnover rates of new soil C. Because increased turnover rates of new soil C limit the potential for additional soil C sequestration, the capacity of land ecosystems to slow the rise in atmospheric CO2 concentrations may be smaller than previously assumed.", "keywords": ["roots", "0106 biological sciences", "570", "550", "soil respiration", "01 natural sciences", "Carbon Cycle", "Soil", "atmospheric carbon dioxide", "XXXXXX - Unknown", "soil carbon", "soils", "isotopes", "Ecosystem", "0105 earth and related environmental sciences", "2. Zero hunger", "carbon", "turnover", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Plants", "15. Life on land", "Carbon", "meta-analysis", "roots (botany)", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "respiration"]}, "links": [{"href": "https://doi.org/10.1111/gcb.13752"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.13752", "name": "item", "description": "10.1111/gcb.13752", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.13752"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-06-02T00:00:00Z"}}, {"id": "10.1111/j.1365-2435.2009.01683.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:31Z", "type": "Journal Article", "created": "2010-01-28", "title": "Impacts Of Experimentally Imposed Drought On Leaf Respiration And Morphology In An Amazon Rain Forest", "description": "Summary1.\uffe2\uff80\uff82The Amazon region may experience increasing moisture limitation over this century. Leaf dark respiration (R) is a key component of the Amazon rain forest carbon (C) cycle, but relatively little is known about its sensitivity to drought.
2.\uffe2\uff80\uff82Here, we present measurements of R standardized to 25\uffe2\uff80\uff83\uffc2\uffb0C and leaf morphology from different canopy heights over 5\uffe2\uff80\uff83years at a rain forest subject to a large\uffe2\uff80\uff90scale through\uffe2\uff80\uff90fall reduction (TFR) experiment, and nearby, unmodified Control forest, at the Caxiuan\uffc3\uffa3 reserve in the eastern Amazon.
3.\uffe2\uff80\uff82In all five post\uffe2\uff80\uff90treatment measurement campaigns, mean R at 25\uffe2\uff80\uff83\uffc2\uffb0C was elevated in the TFR forest compared to the Control forest experiencing normal rainfall. After 5\uffe2\uff80\uff83years of the TFR treatment, R per unit leaf area and mass had increased by 65% and 42%, respectively, relative to pre\uffe2\uff80\uff90treatment means. In contrast, leaf area index (L) in the TFR forest was consistently lower than the Control, falling by 23% compared to the pre\uffe2\uff80\uff90treatment mean, largely because of a decline in specific leaf area (S).
4.\uffe2\uff80\uff82The consistent and significant effects of the TFR treatment on R, L and S suggest that severe drought events in the Amazon, of the kind that may occur more frequently in future, could cause a substantial increase in canopy carbon dioxide emissions from this ecosystem to the atmosphere.
", "keywords": ["tropical forest", "0301 basic medicine", "Through-fall exclusion experiment", "moisture transfer", "03 medical and health sciences", "Specific leaf area", "Amazonia", "Tropical forest", "Keywords: carbon cycle", "Climate change", "Para [Brazil] Climate change", "Caxiuana National Forest", "0303 health sciences", "leaf area index", "Night-time foliar carbon emissions", "exclusion experiment", "15. Life on land", "6. Clean water", "Leaf dark respiration", "forest canopy", "Moisture deficit", "climate change", "13. Climate action", "Leaf area index", "carbon emission", "throughfall", "rainforest", "Brazil"]}, "links": [{"href": "https://openresearch-repository.anu.edu.au/bitstream/1885/79379/5/f5625xPUB7833.pdf.jpg"}, {"href": "https://openresearch-repository.anu.edu.au/bitstream/1885/79379/7/01_Metcalfe_Impacts_of_experimentally_2010.pdf.jpg"}, {"href": "https://doi.org/10.1111/j.1365-2435.2009.01683.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Functional%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2435.2009.01683.x", "name": "item", "description": "10.1111/j.1365-2435.2009.01683.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2435.2009.01683.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-04-08T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2004.00729.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:32Z", "type": "Journal Article", "created": "2004-12-24", "title": "Root Production Is Determined By Radiation Flux In A Temperate Grassland Community", "description": "AbstractAccurate knowledge of the response of root turnover to a changing climate is needed to predict growth and produce carbon cycle models. A soil warming system and shading were used to vary soil temperature and received radiation independently in a temperate grassland dominated by Holcus lanatus L. Minirhizotrons allowed root growth and turnover to be examined non\uffe2\uff80\uff90destructively. In two short\uffe2\uff80\uff90term (8 week) experiments, root responses to temperature were seasonally distinct. Root number increased when heating was applied during spring, but root death increased during autumnal heating. An experiment lasting 12 months demonstrated that any positive response to temperature was short\uffe2\uff80\uff90lived and that over a full growing season, soil warming led to a reduction in root number and mass due to increased root death during autumn and winter. Root respiration was also insensitive to soil temperature over much of the year. In contrast, root growth was strongly affected by incident radiation. Root biomass, length, birth rate, number and turnover were all reduced by shading. Photosynthesis in H. lanatus exhibited some acclimation to shading, but assimilation rates at growth irradiance were still lower in shaded plants. The negative effects of shading and soil warming on roots were additive. Comparison of root data with environmental measurements demonstrated a number of positive relationships with photosynthetically active radiation, but not with soil temperature. This was true both across the entire data set and within a shade treatment. These results demonstrate that root growth is unlikely to be directly affected by increased soil temperatures as a result of global warming, at least in temperate areas, and that predictions of net primary productivity should not be based on a positive root growth response to temperature.
", "keywords": ["Plantago lanceolata Acclimation", "Root respiration", "belowground production", "soil temperature", "warming", "Belowground net primary production", "550", "Received photosynthetically active radiation", "Root turnover", "Plantago lanceolata", "photosynthetically active radiation", "Plantago", "580", "2. Zero hunger", "Root demography", "Temperature", "04 agricultural and veterinary sciences", "15. Life on land", "Minirhizotrons", "Keywords: acclimation", "climate change", "Holcus lanatus", "13. Climate action", "Lanceolata", "Soil warming", "0401 agriculture", " forestry", " and fisheries", "root system", "grassland", "shading", "respiration"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/495/1/fitterah10.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2004.00729.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2004.00729.x", "name": "item", "description": "10.1111/j.1365-2486.2004.00729.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2004.00729.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-01-26T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2006.01240.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:33Z", "type": "Journal Article", "created": "2006-09-26", "title": "Interactions between plant growth and soil nutrient cycling under elevated CO2: a meta-analysis", "description": "Abstractfree air carbon dioxide enrichment (FACE) and open top chamber (OTC) studies are valuable tools for evaluating the impact of elevated atmospheric CO2 on nutrient cycling in terrestrial ecosystems. Using meta\uffe2\uff80\uff90analytic techniques, we summarized the results of 117 studies on plant biomass production, soil organic matter dynamics and biological N2 fixation in FACE and OTC experiments. The objective of the analysis was to determine whether elevated CO2 alters nutrient cycling between plants and soil and if so, what the implications are for soil carbon (C) sequestration. Elevated CO2 stimulated gross N immobilization by 22%, whereas gross and net N mineralization rates remained unaffected. In addition, the soil C\uffe2\uff80\uff83:\uffe2\uff80\uff83N ratio and microbial N contents increased under elevated CO2 by 3.8% and 5.8%, respectively. Microbial C contents and soil respiration increased by 7.1% and 17.7%, respectively. Despite the stimulation of microbial activity, soil C input still caused soil C contents to increase by 1.2%\uffe2\uff80\uff83yr\uffe2\uff88\uff921. Namely, elevated CO2 stimulated overall above\uffe2\uff80\uff90 and belowground plant biomass by 21.5% and 28.3%, respectively, thereby outweighing the increase in CO2 respiration. In addition, when comparing experiments under both low and high N availability, soil C contents (+2.2%\uffe2\uff80\uff83yr\uffe2\uff88\uff921) and above\uffe2\uff80\uff90 and belowground plant growth (+20.1% and+33.7%) only increased under elevated CO2 in experiments receiving the high N treatments. Under low N availability, above\uffe2\uff80\uff90 and belowground plant growth increased by only 8.8% and 14.6%, and soil C contents did not increase. Nitrogen fixation was stimulated by elevated CO2 only when additional nutrients were supplied. These results suggest that the main driver of soil C sequestration is soil C input through plant growth, which is strongly controlled by nutrient availability. In unfertilized ecosystems, microbial N immobilization enhances acclimation of plant growth to elevated CO2 in the long\uffe2\uff80\uff90term. Therefore, increased soil C input and soil C sequestration under elevated CO2 can only be sustained in the long\uffe2\uff80\uff90term when additional nutrients are supplied.
", "keywords": ["2. Zero hunger", "enrichment", "microbial biomass", "atmospheric carbon-dioxide", "nitrogen-fixation", "dynamics", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "forest", "tallgrass prairie", "13. Climate action", "responses", "0401 agriculture", " forestry", " and fisheries", "organic-matter", "respiration"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2006.01240.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2006.01240.x", "name": "item", "description": "10.1111/j.1365-2486.2006.01240.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2006.01240.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-09-26T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2006.01146.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:33Z", "type": "Journal Article", "created": "2006-04-03", "title": "Soil Carbon Balance In A Clonal Eucalyptus Plantation In Congo: Effects Of Logging On Carbon Inputs And Soil Co2 Efflux", "description": "AbstractSoil CO2 efflux was measured in clear\uffe2\uff80\uff90cut and intact plots in order to quantify the impact of harvest on soil respiration in an intensively managed Eucalyptus plantation, and to evaluate the increase in heterotrophic component of soil respiration because of the decomposition of harvest residues. Soil CO2 effluxes showed a pronounced seasonal trend, which was well related to the pattern of precipitation and soil water content and were always significantly lower in the clear\uffe2\uff80\uff90cut plots than in the intact plots. On an annual basis, soil respiration represented 1.57 and 0.91\uffe2\uff80\uff83kgC\uffe2\uff80\uff83m\uffe2\uff88\uff922\uffe2\uff80\uff83yr\uffe2\uff88\uff921 in intact and clear\uffe2\uff80\uff90cut plots, respectively. During the first year following harvest, residues have lost 0.79\uffe2\uff80\uff83kgC\uffe2\uff80\uff83m\uffe2\uff88\uff922\uffe2\uff80\uff83yr\uffe2\uff88\uff921. Our estimate of heterotrophic respiration was calculated assuming that it was similar to soil respiration in the clear\uffe2\uff80\uff90cut area except that the decomposition of residues did not occur, and it was further corrected for differences in soil water content between intact and clear\uffe2\uff80\uff90cut plots and for the cessation of leaf and fine root turnover in clear cut. Heterotrophic respiration in clear\uffe2\uff80\uff90cut plots was estimated at 1.18\uffe2\uff80\uff83kgC\uffe2\uff80\uff83m\uffe2\uff88\uff922\uffe2\uff80\uff83yr\uffe2\uff88\uff921 whereas it was only 0.65\uffe2\uff80\uff83kgC\uffe2\uff80\uff83m\uffe2\uff88\uff922\uffe2\uff80\uff83yr\uffe2\uff88\uff921 in intact plots (41% of soil respiration). Assumptions and uncertainties with these calculations are discussed.
", "keywords": ["DECOMPOSITION", "0106 biological sciences", "550", "[SDE.MCG]Environmental Sciences/Global Changes", "F60 - Physiologie et biochimie v\u00e9g\u00e9tale", "FOREST MANAGEMENT", "01 natural sciences", "EUCALYPTUS", "http://aims.fao.org/aos/agrovoc/c_1301", "http://aims.fao.org/aos/agrovoc/c_2159", "http://aims.fao.org/aos/agrovoc/c_3047", "CLEAR-CUT", "2. Zero hunger", "Eucalyptus", "liti\u00e8re foresti\u00e8re", "http://aims.fao.org/aos/agrovoc/c_2847", "abattage d'arbres", "04 agricultural and veterinary sciences", "15. Life on land", "CARBON BUDGET", "[SDE.MCG] Environmental Sciences/Global Changes", "LITTERFALL", "d\u00e9gradation", "0401 agriculture", " forestry", " and fisheries", "carbone", "SOIL RESPIRATION", "http://aims.fao.org/aos/agrovoc/c_8500", "http://aims.fao.org/aos/agrovoc/c_2683"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2006.01146.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2006.01146.x", "name": "item", "description": "10.1111/j.1365-2486.2006.01146.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2006.01146.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-04-03T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2008.01793.x", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-06-23T16:19:35Z", "type": "Journal Article", "created": "2008-11-03", "title": "Precipitation Pulses Enhance Respiration Of Mediterranean Ecosystems: The Balance Between Organic And Inorganic Components Of Increased Soil Co2efflux", "description": "AbstractIn regions characterized by arid seasons, such as the Mediterranean basin, soil moisture is a major driver of ecosystem CO2 efflux during periods of drought stress. Here, a rain event can induce a disproportional respiratory pulse, releasing an amount of CO2 to the atmosphere that may significantly contribute to the annual ecosystem carbon balance. The mechanisms behind this pulse are unclear, and it is still unknown whether it is due to the stimulation of autotrophic, heterotrophic and/or inorganic CO2 fluxes. On the Mediterranean island of Pianosa, eddy flux measurements showed respiratory pulses after rain events following prolonged drought periods, which occurred in the summer of 2003 and 2006. To investigate the mechanisms of this observed enhanced respiration fluxes and partition of the soil CO2 sources, two water manipulation experiments were performed. The first was designed to estimate the effect of soil rewetting on soil CO2 efflux, in the different ecosystem types existing on the island (i.e. woodland, ex\uffe2\uff80\uff90agricultural and Mediterranean shrubland). The second was a soil CO2 partitioning experiment to investigate the relative contribution of inorganic and organic CO2 sources to soil respiration, under dry and wet soil conditions. Our results suggest that the pulse in the CO2 efflux is primarily due to the enhancement of heterotrophic respiration, likely caused by the degradation of easily decomposable substrates, accumulated in soils during the dry period. In fact, the vegetation at the site was senescent and did not play any significant role in CO2 exchange, as suggested by the absence of diurnal CO2 uptake in eddy covariance measurements. In addition, soil rewetting did not significantly enhance inorganic CO2 efflux.
", "keywords": ["13. Climate action", "mediterranean", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "precipitation", "15. Life on land", "ecosystems", "respiration", "6. Clean water", "components"]}, "links": [{"href": "https://air.uniud.it/bitstream/11390/864899/1/Inglima_et_al_2009.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2008.01793.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2008.01793.x", "name": "item", "description": "10.1111/j.1365-2486.2008.01793.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2008.01793.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-04-07T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2004.00737.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:32Z", "type": "Journal Article", "created": "2004-12-24", "title": "Simulated Chronic No3\u2212Deposition Reduces Soil Respiration In Northern Hardwood Forests", "description": "AbstractChronic N additions to forest ecosystems can enhance soil N availability, potentially leading to reduced C allocation to root systems. This in turn could decrease soil CO2 efflux. We measured soil respiration during the first, fifth, sixth and eighth years of simulated atmospheric NO3\uffe2\uff88\uff92 deposition (3\uffe2\uff80\uff83g\uffe2\uff80\uff83N\uffe2\uff80\uff83m\uffe2\uff88\uff922\uffe2\uff80\uff83yr\uffe2\uff88\uff921) to four sugar maple\uffe2\uff80\uff90dominated northern hardwood forests in Michigan to assess these possibilities. During the first year, soil respiration rates were slightly, but not significantly, higher in the NO3\uffe2\uff88\uff92\uffe2\uff80\uff90amended plots. In all subsequent measurement years, soil respiration rates from NO3\uffe2\uff88\uff92\uffe2\uff80\uff90amended soils were significantly depressed. Soil temperature and soil matric potential were measured concurrently with soil respiration and used to develop regression relationships for predicting soil respiration rates. Estimates of growing season and annual soil CO2 efflux made using these relationships indicate that these C fluxes were depressed by 15% in the eighth year of chronic NO3\uffe2\uff88\uff92 additions. The decrease in soil respiration was not due to reduced C allocation to roots, as root respiration rates, root biomass, and root turnover were not significantly affected by N additions. Aboveground litter also was unchanged by the 8 years of treatment. Of the remaining potential causes for the decline in soil CO2 efflux, reduced microbial respiration appears to be the most likely possibility. Documented reductions in microbial biomass and the activities of extracellular enzymes used for litter degradation on the NO3\uffe2\uff88\uff92\uffe2\uff80\uff90amended plots are consistent with this explanation.
", "keywords": ["Nitrogen Fertilization", "Soil CO 2 Efflux", "Geology and Earth Sciences", "Science", "Atmospheric Nitrate Deposition", "Ecology and Evolutionary Biology", "Root Respiration", "0401 agriculture", " forestry", " and fisheries", "Temperature and Moisture Effects", "04 agricultural and veterinary sciences", "15. Life on land", "Root Biomass"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2004.00737.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2004.00737.x", "name": "item", "description": "10.1111/j.1365-2486.2004.00737.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2004.00737.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-05-07T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2004.00868.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:32Z", "type": "Journal Article", "created": "2004-12-02", "title": "Experimental Warming And Burn Severity Alter Soil Co2 Flux And Soil Functional Groups In A Recently Burned Boreal Forest", "description": "AbstractGlobal warming is projected to be greatest in northern regions, where forest fires are also increasing in frequency. Thus, interactions between fire and temperature on soil respiration at high latitudes should be considered in determining feedbacks to climate. We tested the hypothesis that experimental warming will augment soil CO2 flux in a recently burned boreal forest by promoting microbial and root growth, but that this increase will be less apparent in more severely burned areas. We used open\uffe2\uff80\uff90top chambers to raise temperatures 0.4\uffe2\uff80\uff930.9\uffc2\uffb0C across two levels of burn severity in a fire scar in Alaskan black spruce forest. After 3 consecutive years of warming, soil respiration was measured through a portable gas exchange system. Abundance of active microbes was determined by using Biolog EcoPlates\uffe2\uff84\uffa2 for bacteria and ergosterol analysis for fungi. Elevated temperatures increased soil CO2 flux by 20% and reduced root biomass, but had no effect on bacterial or fungal abundance or soil organic matter (SOM) content. Soil respiration, fungal abundance, SOM, and root biomass decreased with increasing burn severity. There were no significant interactions between temperature and burn severity with respect to any measurement. Higher soil respiration rates in the warmed plots may be because of higher metabolic activity of microbes or roots. All together, we found that postfire soils are a greater source of CO2 to the atmosphere under elevated temperatures even in severely burned areas, suggesting that global warming may produce a positive feedback to atmospheric CO2, even in young boreal ecosystems.
", "keywords": ["warming", "carbon", "temperature", "04 agricultural and veterinary sciences", "15. Life on land", "root", "01 natural sciences", "soil", "microbe", "storage", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "fungi", "bacteria", "respiration", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://escholarship.org/content/qt8qh265s2/qt8qh265s2.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2004.00868.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2004.00868.x", "name": "item", "description": "10.1111/j.1365-2486.2004.00868.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2004.00868.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-12-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2005.001058.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:32Z", "type": "Journal Article", "created": "2005-11-28", "title": "Effects Of Experimental Drought On Soil Respiration And Radiocarbon Efflux From A Temperate Forest Soil", "description": "AbstractSoil moisture affects microbial decay of SOM and rhizosphere respiration (RR) in temperate forest soils, but isolating the response of soil respiration (SR) to summer drought and subsequent wetting is difficult because moisture changes are often confounded with temperature variation. We distinguished between temperature and moisture effects by simulation of prolonged soil droughts in a mixed deciduous forest at the Harvard Forest, Massachusetts. Roofs constructed over triplicate 5 \uffc3\uff97 5\uffe2\uff80\uff83m2plots excluded throughfall water during the summers of 2001 (168\uffe2\uff80\uff83mm) and 2002 (344\uffe2\uff80\uff83mm), while adjacent control plots received ambient throughfall and the same natural temperature regime. In 2003, throughfall was not excluded to assess the response of SR under natural weather conditions after two prolonged summer droughts. Throughfall exclusion significantly decreased mean SR rate by 53\uffe2\uff80\uff83mg\uffe2\uff80\uff83C\uffe2\uff80\uff83m\uffe2\uff88\uff922\uffe2\uff80\uff83h\uffe2\uff88\uff921over 84 days in 2001, and by 68\uffe2\uff80\uff83mg\uffe2\uff80\uff83C\uffe2\uff80\uff83m\uffe2\uff88\uff922\uffe2\uff80\uff83h\uffe2\uff88\uff921over 126 days in 2002, representing 10\uffe2\uff80\uff9330% of annual SR in this forest and 35\uffe2\uff80\uff9375% of annual net ecosystem exchange (NEE) of C. The differences in SR were best explained by differences in gravimetric water content in the Oi horizon (r2=0.69) and the Oe/Oa horizon (r2=0.60). Volumetric water content of the A horizon was not significantly affected by throughfall exclusion. The radiocarbon signature of soil CO2efflux and of CO2respired during incubations of O horizon, A horizon and living roots allowed partitioning of SR into contributions from young C substrate (including RR) and from decomposition of older SOM. RR (root respiration and microbial respiration of young substrates in the rhizosphere) made up 43\uffe2\uff80\uff9371% of the total C respired in the control plots and 41\uffe2\uff80\uff9380% in the exclusion plots, and tended to increase with drought. An exception to this trend was an interesting increase in CO2efflux of radiocarbon\uffe2\uff80\uff90rich substrates during a period of abundant growth of mushrooms.
Our results suggest that prolonged summer droughts decrease primarily heterotrophic respiration in the O horizon, which could cause increases in the storage of soil organic carbon in this forest. However, the C stored during two summers of simulated drought was only partly released as increased respiration during the following summer of natural throughfall. We do not know if this soil C sink during drought is transient or long lasting. In any case, differential decomposition of the O horizon caused by interannual variation of precipitation probably contributes significantly to observed interannual variation of NEE in temperate forests.
", "keywords": ["Ecology", "04 agricultural and veterinary sciences", "Biological Sciences", "15. Life on land", "soil respiration", "6. Clean water", "soil drought", "heterotrophic respiration", "rhizosphere respiration", "13. Climate action", "soil organic matter", "temperate forest", "radiocarbon", "0401 agriculture", " forestry", " and fisheries", "soil wetting", "soil moisture", "Q(10)", "Environmental Sciences"]}, "links": [{"href": "https://escholarship.org/content/qt3mk9v58k/qt3mk9v58k.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2005.001058.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2005.001058.x", "name": "item", "description": "10.1111/j.1365-2486.2005.001058.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2005.001058.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-11-28T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2007.01415.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:34Z", "type": "Journal Article", "created": "2007-08-18", "title": "Microbial Soil Respiration And Its Dependency On Carbon Inputs, Soil Temperature And Moisture", "description": "AbstractThis experiment was designed to study three determinant factors in decomposition patterns of soil organic matter (SOM): temperature, water and carbon (C) inputs. The study combined field measurements with soil lab incubations and ends with a modelling framework based on the results obtained. Soil respiration was periodically measured at an oak savanna woodland and a ponderosa pine plantation. Intact soils cores were collected at both ecosystems, including soils with most labile C burnt off, soils with some labile C gone and soils with fresh inputs of labile C. Two treatments, dry\uffe2\uff80\uff90field condition and field capacity, were applied to an incubation that lasted 111 days. Short\uffe2\uff80\uff90term temperature changes were applied to the soils periodically to quantify temperature responses. This was done to prevent confounding results associated with different pools of C that would result by exposing treatments chronically to different temperature regimes. This paper discusses the role of the above\uffe2\uff80\uff90defined environmental factors on the variability of soil C dynamics. At the seasonal scale, temperature and water were, respectively, the main limiting factors controlling soil CO2 efflux for the ponderosa pine and the oak savanna ecosystems. Spatial and seasonal variations in plant activity (root respiration and exudates production) exerted a strong influence over the seasonal and spatial variation of soil metabolic activity. Mean residence times of bulk SOM were significantly lower at the Nitrogen (N)\uffe2\uff80\uff90rich deciduous savanna than at the N\uffe2\uff80\uff90limited evergreen dominated pine ecosystem. At shorter time scales (daily), SOM decomposition was controlled primarily by temperature during wet periods and by the combined effect of water and temperature during dry periods. Secondary control was provided by the presence/absence of plant derived C inputs (exudation). Further analyses of SOM decomposition suggest that factors such as changes in the decomposer community, stress\uffe2\uff80\uff90induced changes in the metabolic activity of decomposers or SOM stabilization patterns remain unresolved, but should also be considered in future SOM decomposition studies. Observations and confounding factors associated with SOM decomposition patterns and its temperature sensitivity are summarized in the modeling framework.
", "keywords": ["2. Zero hunger", "Soil organic matter", "Climate change", "Cambio clim\u00e1tico", "0401 agriculture", " forestry", " and fisheries", "Soil respiration", "04 agricultural and veterinary sciences", "15. Life on land", "Materia org\u00e1nica del suelo", "Respiraci\u00f3n del suelo"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2007.01415.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2007.01415.x", "name": "item", "description": "10.1111/j.1365-2486.2007.01415.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2007.01415.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-07-21T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2008.01549.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:35Z", "type": "Journal Article", "created": "2008-02-11", "title": "Microbial Activity And Soil Respiration Under Nitrogen Addition In Alaskan Boreal Forest", "description": "AbstractClimate warming could increase rates of soil organic matter turnover and nutrient mineralization, particularly in northern high\uffe2\uff80\uff90latitude ecosystems. However, the effects of increasing nutrient availability on microbial processes in these ecosystems are poorly understood. To determine how soil microbes respond to nutrient enrichment, we measured microbial biomass, extracellular enzyme activities, soil respiration, and the community composition of active fungi in nitrogen (N) fertilized soils of a boreal forest in central Alaska. We predicted that N addition would suppress fungal activity relative to bacteria, but stimulate carbon (C)\uffe2\uff80\uff90degrading enzyme activities and soil respiration. Instead, we found no evidence for a suppression of fungal activity, although fungal sporocarp production declined significantly, and the relative abundance of two fungal taxa changed dramatically with N fertilization. Microbial biomass as measured by chloroform fumigation did not respond to fertilization, nor did the ratio of fungi\uffe2\uff80\uff83:\uffe2\uff80\uff83bacteria as measured by quantitative polymerase chain reaction. However, microbial biomass C\uffe2\uff80\uff83:\uffe2\uff80\uff83N ratios narrowed significantly from 16.0 \uffc2\uffb1 1.4 to 5.2 \uffc2\uffb1 0.3 with fertilization. N fertilization significantly increased the activity of a cellulose\uffe2\uff80\uff90degrading enzyme and suppressed the activities of protein\uffe2\uff80\uff90 and chitin\uffe2\uff80\uff90degrading enzymes but had no effect on soil respiration rates or 14C signatures. These results indicate that N fertilization alters microbial community composition and allocation to extracellular enzyme production without affecting soil respiration. Thus, our results do not provide evidence for strong microbial feedbacks to the boreal C cycle under climate warming or N addition. However, organic N cycling may decline due to a reduction in the activity of enzymes that target nitrogenous compounds.
", "keywords": ["2. Zero hunger", "nucleotide analog", "Ecology", "microbial biomass", "ectomycorrhizal fungi", "extracellular enzyme", "nitrogen fertilization", "04 agricultural and veterinary sciences", "15. Life on land", "Biological Sciences", "soil respiration", "Environmental sciences", "Biological sciences", "Earth sciences", "13. Climate action", "carbon cycle", "0401 agriculture", " forestry", " and fisheries", "boreal forest", "bacteria", "Alaska", "Environmental Sciences"]}, "links": [{"href": "https://escholarship.org/content/qt5dg6p7gm/qt5dg6p7gm.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2008.01549.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2008.01549.x", "name": "item", "description": "10.1111/j.1365-2486.2008.01549.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2008.01549.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-01-20T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Respiration&offset=50&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Respiration&offset=50&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "prev", "title": "items (prev)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Respiration&offset=0", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Respiration&offset=100", "hreflang": "en-US"}], "numberMatched": 223, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-06-24T15:13:33.014682Z"}