{"type": "FeatureCollection", "features": [{"id": "10.3389/fpls.2019.00045", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2019-01-29", "title": "Construction of a High-Density Genetic Map and Identification of Loci Related to Hollow Stem Trait in Broccoli (Brassic oleracea L. italica)", "description": "A high-quality genetic map is important for mapping of compound traits. In this study, a genetic map was constructed based on the reference genome TO1000 after specific locus amplified fragment (SLAF) sequencing in a double-haploid segregation population of broccoli, and loci controlling hollow stem trait were identified in the genetic map. The genetic map contains 4,787 SLAF markers, with a mean marker distance of 0.22 cM and the mean sequencing depths of 91.14-fold in the maternal line, 88.97-fold in the paternal line and 17.11-fold in each DH progeny. A locus controlling the hollow stem trait, QHS.C09-2, which could explain 14.1% of the phenotypic variation, was steadily detected on the linkage group nine in the indicated data of 3 years' trials and BLUE analysis. The genetic map could lay an important foundation for mapping of compound traits, and mapping of hollow stem trait would be basis to clone the genes related to hollow stems in broccoli.", "keywords": ["broccoli", "0301 basic medicine", "locus", "0303 health sciences", "Locus", "Broccoli; Genetic map; Hollow stems; Locus; SLAF; Plant Science", "Broccoli", "Plant culture", "Plant Science", "hollow stems", "SB1-1110", "03 medical and health sciences", "Genetic map", "Hollow stems", "genetic map", "SLAF"]}, "links": [{"href": "https://www.iris.unict.it/bitstream/20.500.11769/361610/5/fpls-10-00045.pdf"}, {"href": "https://doi.org/10.3389/fpls.2019.00045"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2019.00045", "name": "item", "description": "10.3389/fpls.2019.00045", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2019.00045"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-01-29T00:00:00Z"}}, {"id": "10.31219/osf.io/jfdb9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:20:57Z", "type": "Journal Article", "created": "2021-03-15", "title": "Overcoming the challenges to enhancing experimental plant biology with computational modeling", "description": "

The study of complex biological systems necessitates computational modeling approaches that are currently underutilized in plant biology. Many plant biologists have trouble identifying or adopting modeling methods to their research, particularly mechanistic mathematical modeling. Here we address challenges that limit the use of computational modeling methods, particularly mechanistic mathematical modeling. We divide computational modeling techniques into either pattern models (e.g., bioinformatics, machine learning, or morphology) or mechanistic mathematical models (e.g., biochemical reactions, biophysics, or population models), which both contribute to plant biology research at different scales to answer different research questions. We present arguments and recommendations for the increased adoption of modeling by plant biologists interested in incorporating more modeling into their research programs. As some researchers find math and quantitative methods to be an obstacle to modeling, we provide suggestions for easy-to-use tools for non-specialists and for collaboration with specialists. This may especially be the case for mechanistic mathematical modeling, and we spend some extra time discussing this. Through a more thorough appreciation and awareness of the power of different kinds of modeling in plant biology, we hope to facilitate interdisciplinary, transformative research.

", "keywords": ["Other Physical Sciences and Mathematics", "computational modeling", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "experimental design", "Systems Biology", "Plant Sciences", "Research Methods in Life Sciences", "mathematical modeling", "Life Sciences", "Plant culture", "bioinformatics", "Plant Science", "collaboration", "SB1-1110", "03 medical and health sciences", "Other Life Sciences", "Physical Sciences and Mathematics"]}, "links": [{"href": "https://doi.org/10.31219/osf.io/jfdb9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.31219/osf.io/jfdb9", "name": "item", "description": "10.31219/osf.io/jfdb9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.31219/osf.io/jfdb9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-15T00:00:00Z"}}, {"id": "10.3389/fagro.2020.605655", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:20:59Z", "type": "Journal Article", "created": "2020-12-04", "title": "Combining Seed Dressing and Foliar Applications of Phosphorus Fertilizer Can Give Similar Crop Growth and Yield Benefits to Soil Applications Together With Greater Recovery Rates", "description": "

Phosphorus (P) fertilizers have a dramatic effect on agricultural productivity, but conventional methods of application result in only limited recovery of the applied P. Given the increasing volatility in rock phosphate prices, more efficient strategies for P fertilizer use would be of economic and environmental benefit in the drive for sustainable intensification. This study used a combination of controlled-environment experiments and radioisotopic labeling to investigate the fertilizer use efficiency of a combination of seed (grain) dressing and foliar applications of P to spring wheat (Triticum aestivumL.). Radioisotopic labeling showed that the application of foliar P in the presence of photosynthetic light substantially increased both P-uptake into the leaf and P-mobilization within the plant, especially when an adjuvant was used. When compared with soil application of inorganic P buried into the rooting zone, a combination of a 3 \uffce\uffbcmol seed dressing and three successive 46.3 \uffce\uffbcmol plant\uffe2\uff88\uff921foliar applications were far more efficient at providing P fertilization benefits in P-limiting conditions. We conclude that a combination of seed dressing and foliar applications of P is potentially a better alternative to conventional soil-based application, offering greater efficiency in use of applied P both in terms of P-uptake rate and grain yield. Further work is required to evaluate whether these results can be obtained under a range of field conditions.Weed control is necessary to ensure a high crop yield with good quality. Herbicide application and mechanical weeding are the most common methods worldwide. The use of herbicides has led to the increasing occurrence of herbicide-resistant weeds and unwanted contamination of the environment. Mechanical weed control harms beneficial organisms, increases the degradation of organic matter, may dry out the soil, and stimulate new cohorts of weed seeds to germinate. Therefore, there is a need to develop more sustainable weed control means. We suggest using small autonomous vehicles equipped with lasers as a sustainable alternative method. Laser beams are based on electricity, which can be produced from non-fossil fuels. Deep learning methods can be used to locate and identify weed and crop plants for targeting and delivery of laser energy with robotic actuators. Given the targeted nature of laser beams, the area exposed for weed control can be reduced substantially compared to commonly used weed control methods. Therefore, the risk of affecting non-target organisms is minimized, and the soil will be kept untouched in the field, avoiding triggering weed seeds to germinate. Small autonomous vehicles may have limited weeding capacity, and precautions need to be taken as reflections from the laser beam can be harmful to humans and animals. In this paper, we discuss the pros and cons of replacing or supplementing common used weed control methods with laser weeding. The ability to use laser weeding technology is relatively new and not yet widely practiced or commercially available. Therefore, we do not discuss and compare the costs of the various methods at this early stage of the development of the technology.

", "keywords": ["2. Zero hunger", "S", "alternative weed control", " integrated weed management", " non-chemical weed control", " site-specific weed management", " thermal weed control", " weed killers", "non-chemical weed control", "Plant culture", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "SB1-1110", "thermal weed control", "integrated weed management", "13. Climate action", "site-specific weed management", "0401 agriculture", " forestry", " and fisheries", "weed killers", "alternative weed control"], "contacts": [{"organization": "Andreasen Christian, Scholle Karsten, Saberi Mahin,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fagro.2022.841086"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fagro.2022.841086", "name": "item", "description": "10.3389/fagro.2022.841086", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fagro.2022.841086"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-07T00:00:00Z"}}, {"id": "10.3389/fpls.2015.00574", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2015-08-11", "title": "Leaf \u03b415N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated [CO2], temperature and low water availability", "description": "The natural (15)N/(14)N isotope composition (\u03b4(15)N) of a tissue is a consequence of its N source and N physiological mechanisms in response to the environment. It could potentially be used as a tracer of N metabolism in plants under changing environmental conditions, where primary N metabolism may be complex, and losses and gains of N fluctuate over time. In order to test the utility of \u03b4(15)N as an indicator of plant N status in N2-fixing plants grown under various environmental conditions, alfalfa (Medicago sativa L.) plants were subjected to distinct conditions of [CO2] (400 vs. 700 \u03bcmol mol(-1)), temperature (ambient vs. ambient +4\u00b0C) and water availability (fully watered vs. water deficiency-WD). As expected, increased [CO2] and temperature stimulated photosynthetic rates and plant growth, whereas these parameters were negatively affected by WD. The determination of \u03b4(15)N in leaves, stems, roots, and nodules showed that leaves were the most representative organs of the plant response to increased [CO2] and WD. Depletion of heavier N isotopes in plants grown under higher [CO2] and WD conditions reflected decreased transpiration rates, but could also be related to a higher N demand in leaves, as suggested by the decreased leaf N and total soluble protein (TSP) contents detected at 700 \u03bcmol mol(-1) [CO2] and WD conditions. In summary, leaf \u03b4(15)N provides relevant information integrating parameters which condition plant responsiveness (e.g., photosynthesis, TSP, N demand, and water transpiration) to environmental conditions.", "keywords": ["0106 biological sciences", "0301 basic medicine", "Physiology", "growth", "Climate Change", "Plant physiology", "Plantes", "Growth", "Plant Science", "01 natural sciences", "SB1-1110", "03 medical and health sciences", "Climate change", "2. Zero hunger", "Alfalfa", "Plant culture", "Plants", "15. Life on land", "delta15N", "6. Clean water", "climate change", "Fisiologia vegetal", "Carbon dioxide", "physiology", "Di\u00f2xid de carboni", "alfalfa", "\u03b415N"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2015.00574"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2015.00574", "name": "item", "description": "10.3389/fpls.2015.00574", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2015.00574"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-08-11T00:00:00Z"}}, {"id": "10.3389/fpls.2015.01010", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2015-11-20", "title": "Elevated Co2 Reduced Floret Death In Wheat Under Warmer Average Temperatures And Terminal Drought", "description": "Elevated CO2 often increases grain yield in wheat by enhancing grain number per ear, which can result from an increase in the potential number of florets or a reduction in the death of developed florets. The hypotheses that elevated CO2 reduces floret death rather than increases floret development, and that grain size in a genotype with more grains per unit area is limited by the rate of grain filling, were tested in a pair of sister lines contrasting in tillering capacity (restricted- vs. free-tillering). The hypotheses were tested under elevated CO2, combined with +3\u00b0C above ambient temperature and terminal drought, using specialized field tunnel houses. Elevated CO2 increased net leaf photosynthetic rates and likely the availability of carbon assimilates, which significantly reduced the rates of floret death and increased the potential number of grains at anthesis in both sister lines by an average of 42%. The restricted-tillering line had faster grain-filling rates than the free-tillering line because the free-tillering line had more grains to fill. Furthermore, grain-filling rates were faster under elevated CO2 and +3\u00b0C above ambient. Terminal drought reduced grain yield in both lines by 19%. Elevated CO2 alone increased the potential number of grains, but a trade-off in yield components limited grain yield in the free-tillering line. This emphasizes the need for breeding cultivars with a greater potential number of florets, since this was not affected by the predicted future climate variables.", "keywords": ["0106 biological sciences", "2. Zero hunger", "restricted tillering", "Climate Change", "Triticum aestivum", "free -tillering", "Restricted tillering", "Plant culture", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "yield", "01 natural sciences", "630", "6. Clean water", "SB1-1110", "compensation", "climate change", "13. Climate action", "florets", "0401 agriculture", " forestry", " and fisheries", "Compensation"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2015.01010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2015.01010", "name": "item", "description": "10.3389/fpls.2015.01010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2015.01010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-11-20T00:00:00Z"}}, {"id": "10.3389/fpls.2016.01730", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2016-11-21", "description": "Tillage operation and fertilizer type play important roles in soil properties as far as soil microbial condition is concerned. Information regarding the simultaneous evaluation of the effect of long-term tillage and fertilization on the soil microbial traits of soybean farms is not available. Accordingly, it was hypothesized that, the microbial biomass and enzyme activity, more often than not, respond quickly to changes in soil tillage and fertilization. Therefore, the experiments were aimed at analyzing the responses of soil microbial traits to tillage and fertilization in a soybean field in Kurdistan University, Iran. The field soil is categorized into coarse Loamy, mixed, superactive, calcareous, and mesic Typic Xerorthents. The experiments were arranged in split plot, based on randomized complete block design with three replications. Main plots consisted of long-term (since 2002) tillage systems including conventional tillage (CT), minimum tillage (MT), and no-tillage (NT). Eight fertilization methods were employed in the sub-plots, including (F1): farmyard manure (FYM); (F2): compost; (F3): chemical fertilizers; (F4): FYM + compost; (F5): FYM + chemical fertilizers; (F6): compost + chemical fertilizers; (F7): FYM + compost + chemical fertilizers and (F8): Control (without fertilizer). The highest microbial biomass carbon (385.1 \u03bcg) was observed in NT-F4 treatment. The NT treatment comparatively recorded higher values of acid phosphatase (189.1 \u03bcg PNP g-1 h-1), alkaline phosphatase (2879.6 \u03bcg PNP g-1 h-1) and dehydrogenase activity (68.1 \u03bcg PNP g-1 h-1). The soil treated with a mixture of compost and FYM inputs had the maximum urease activity of all tillage treatments. Organically manured treatment (F4) showed more activity in dehydrogenase (85.7 \u03bcg PNP g-1 h-1), acid phosphatase (199.1 \u03bcg PNP g-1 h-1), and alkaline phosphatase (3183.6 \u03bcg PNP g-1 h-1) compared to those treated with chemical fertilizers. In NT-F4 treatment, using on-farm inputs is most suitable for sustainable management and improvement in soil biological activities in soybean cultivation. We concluded that applying organic manures and employing reduced tillage systems increased soil microbial biomass and enzyme activities.", "keywords": ["urease", "2. Zero hunger", "compost", "microbial biomass", "Plant culture", "Compost", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "Urease", "6. Clean water", "phosphatase", "SB1-1110", "12. Responsible consumption", "dehydrogenase", "tillage", "0401 agriculture", " forestry", " and fisheries", "tillage."]}, "links": [{"href": "https://doi.org/10.3389/fpls.2016.01730"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2016.01730", "name": "item", "description": "10.3389/fpls.2016.01730", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2016.01730"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-11-21T00:00:00Z"}}, {"id": "10.3389/fpls.2017.00996", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2017-06-20", "description": "Open AccessLas pr\u00e1cticas de agricultura de conservaci\u00f3n (AC) se est\u00e1n promoviendo ampliamente en muchas \u00e1reas del \u00c1frica subsahariana para recuperar los suelos degradados y mejorar los servicios ecosist\u00e9micos. Este estudio examin\u00f3 los efectos de tres pr\u00e1cticas de labranza [arado convencional con vertedera (CT), azada manual (MT) y labranza sin labranza (NT)], y tres sistemas de cultivo (ma\u00edz continuo, rotaci\u00f3n anual de soja-ma\u00edz y cultivo intercalado de soja/ma\u00edz) en la calidad del suelo, la productividad de los cultivos y la rentabilidad en ensayos en finca administrados por investigadores y agricultores de 2010 a 2013 en el noroeste de Ghana. En el ensayo madre gestionado por el investigador, las pr\u00e1cticas de AC de NT, retenci\u00f3n de residuos y rotaci\u00f3n/intercalaci\u00f3n de cultivos mantuvieron un mayor carbono org\u00e1nico del suelo y N total del suelo en comparaci\u00f3n con las pr\u00e1cticas de labranza convencionales despu\u00e9s de 4 a\u00f1os. La densidad aparente del suelo fue mayor en los suelos NT que en los suelos CT en los senderos madre gestionados por el investigador o en los ensayos de beb\u00e9s gestionados por los agricultores despu\u00e9s de 4 a\u00f1os. En el ensayo madre gestionado por el investigador, no hubo diferencias significativas entre los sistemas de labranza o los sistemas de cultivo en los rendimientos de ma\u00edz o soja en las primeras tres temporadas. En la cuarta temporada, la rotaci\u00f3n de cultivos tuvo el mayor impacto en los rendimientos de ma\u00edz con ma\u00edz CT despu\u00e9s de que la soja aumentara los rendimientos en un 41 y 49% en comparaci\u00f3n con el ma\u00edz MT y NT, respectivamente. En los ensayos gestionados por los agricultores, el rendimiento del ma\u00edz oscil\u00f3 entre 520 y 2700 kg ha-1 y entre 300 y 2000 kg ha-1 para CT y NT, respectivamente, lo que refleja las diferencias en la experiencia de los agricultores con NT. En promedio entre los agricultores, los sistemas de cultivo CT aumentaron el rendimiento de ma\u00edz y soja en un rango de 23 a 39% en comparaci\u00f3n con los sistemas de cultivo NT. El an\u00e1lisis parcial del presupuesto mostr\u00f3 que el costo de producir ma\u00edz o soja es 20-29% m\u00e1s barato con los sistemas NT y da mayores rendimientos al trabajo en comparaci\u00f3n con la pr\u00e1ctica de CT. Las relaciones beneficio/coste tambi\u00e9n muestran que los sistemas de cultivo NT son m\u00e1s rentables que los sistemas CT. Concluimos que con el tiempo, la implementaci\u00f3n de pr\u00e1cticas de AC que involucran NT, rotaci\u00f3n de cultivos, cultivos intercalados de ma\u00edz y soja junto con la retenci\u00f3n de residuos de cultivos presenta un escenario de beneficio mutuo debido a la mejora del rendimiento de los cultivos, el aumento del rendimiento econ\u00f3mico y las tendencias de aumento de la fertilidad del suelo. Sin embargo, el mayor desaf\u00edo sigue siendo producir suficiente biomasa y retenerla en el campo.", "keywords": ["Conservation agriculture", "Cropping", "Agricultural Innovation and Livelihood Diversification", "no-tillage", "Soil Science", "Plant Science", "Crop", "Soil quality", "Environmental science", "SB1-1110", "Tillage", "Agricultural and Biological Sciences", "residue retention", "crop rotation", "Crop rotation", "FOS: Mathematics", "profitability", "Crop residue", "Crop yield", "soil quality", "Crop Yield Stability", "Agroforestry", "Biology", "2. Zero hunger", "Conventional tillage", "Geography", "Crop Diversity", "Plant culture", "Life Sciences", "Agriculture", "04 agricultural and veterinary sciences", "crop yield", "Soil Nutrient Management", "15. Life on land", "Plough", "Agronomy", "conservation agriculture", "Intercropping", "Archaeology", "Agricultural science", "0401 agriculture", " forestry", " and fisheries", "Intercropping in Agricultural Systems", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "General Agricultural and Biological Sciences", "intercropping", "Agronomy and Crop Science", "Mathematics", "Cropping system"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2017.00996"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2017.00996", "name": "item", "description": "10.3389/fpls.2017.00996", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2017.00996"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-06-21T00:00:00Z"}}, {"id": "10.3389/fpls.2021.715676", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-07-02", "title": "Editorial: Plant Root Interaction With Associated Microbiomes to Improve Plant Resiliency and Crop Biodiversity", "description": "Plant beneficial microorganisms are now accepted as potential alternatives to chemical fertilizers and pesticides in agriculture. However, despite the enormous research efforts, there is still much to be learnt about the underlying processes that affect the efficacy of biocontrol and biofertilizer products in crop systems. A deeper ecological understanding of complex interactions among introduced beneficial strains and resident microbiota under changing environment is essential (Xu and Jeger, 2020). These complex interactions are greatly influenced by the formulation of beneficial microbial strains (Vassilev and de Oliveira Mendes, 2018) as well as the growing substrate characteristics (Ponzio et al., 2013) and commercial agricultural practices (Malus\u00e1 et al., 2016).", "keywords": ["2. Zero hunger", "0106 biological sciences", "agronomic strategies", "Plant culture", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "soil", "SB1-1110", "plant-associated microbiome", "0401 agriculture", " forestry", " and fisheries", "biocontrol", "biofertilization"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2021.715676"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2021.715676", "name": "item", "description": "10.3389/fpls.2021.715676", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2021.715676"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-07-02T00:00:00Z"}}, {"id": "10.3389/fpls.2017.02260", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2018-01-22", "description": "Agroecosystem nitrogen (N) loss produces greenhouse gases, induces eutrophication, and is costly for farmers; therefore, conservation agricultural management practices aimed at reducing N loss are increasingly adopted. However, the ecosystem consequences of these practices have not been well-studied. We quantified N loss via leaching, NH3 volatilization, N2O emissions, and N retention in plant and soil pools of corn conservation agroecosystems in Kentucky, USA. Three systems were evaluated: (1) an unfertilized, organic system with cover crops hairy vetch (Vicia villosa), winter wheat (Triticum aestivum), or a mix of the two (bi-culture); (2) an organic system with a hairy vetch cover crop employing three fertilization schemes (0 N, organic N, or a fertilizer N-credit approach); and (3) a conventional system with a winter wheat cover crop and three fertilization schemes (0 N, urea N, or organic N). In the unfertilized organic system, cover crop species affected NO3-N leaching (vetch > bi-culture > wheat) and N2O-N emissions and yield during corn growth (vetch, bi-culture > wheat). Fertilization increased soil inorganic N, gaseous N loss, N leaching, and yield in the organic vetch and conventional wheat systems. Fertilizer scheme affected the magnitude of growing season N2O-N loss in the organic vetch system (organic N > fertilizer N-credit) and the timing of loss (organic N delayed N2O-N loss vs. urea) and NO3-N leaching (urea >> organic N) in the conventional wheat system, but had no effect on yield. Cover crop selection and N fertilization techniques can reduce N leaching and greenhouse gas emissions without sacrificing yield, thereby enhancing N conservation in both organic and conventional conservation agriculture systems.", "keywords": ["2. Zero hunger", "nitrous oxide emissions", "Plant culture", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "SB1-1110", "conservation agriculture", "ammonia volatilization", "13. Climate action", "nitrate leaching", "0401 agriculture", " forestry", " and fisheries", "cover crops"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2017.02260"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2017.02260", "name": "item", "description": "10.3389/fpls.2017.02260", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2017.02260"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-22T00:00:00Z"}}, {"id": "10.3389/fpls.2018.01158", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2018-08-08", "title": "Simulation of Soil Organic Carbon Effects on Long-Term Winter Wheat (Triticum aestivum) Production Under Varying Fertilizer Inputs", "description": "Soil organic carbon (SOC) has a vital role to enhance agricultural productivity and for mitigation of climate change. To quantify SOC effects on productivity, process models serve as a robust tool to keep track of multiple plant and soil factors and their interactions affecting SOC dynamics. We used soil-plant-atmospheric model viz. DAISY, to assess effects of SOC on nitrogen (N) supply and plant available water (PAW) under varying N fertilizer rates in winter wheat (Triticum aestivum) in Denmark. The study objective was assessment of SOC effects on winter wheat grain and aboveground biomass accumulation at three SOC levels (low: 0.7% SOC; reference: 1.3% SOC; and high: 2% SOC) with five nitrogen rates (0-200 kg N ha-1) and PAW at low, reference, and high SOC levels. The three SOC levels had significant effects on grain yields and aboveground biomass accumulation at only 0-100 kg N ha-1 and the SOC effects decreased with increasing N rates until no effects at 150-200 kg N ha-1. PAW had significant positive correlation with SOC content, with high SOC retaining higher PAW compared to low and reference SOC. The mean PAW and SOC correlation was given by PAW% = 1.0073 \u00d7 SOC% + 15.641. For the 0.7-2% SOC range, the PAW increase was small with no significant effects on grain yields and aboveground biomass accumulation. The higher winter wheat grain and aboveground biomass was attributed to higher N supply in N deficient wheat production system. Our study suggested that building SOC enhances agronomic productivity at only 0-100 kg N ha-1. Maintenance of SOC stock will require regular replenishment of SOC, to compensate for the mineralization process degrading SOC over time. Hence, management can maximize realization of SOC benefits by building up SOC and maintaining N rates in the range 0-100 kg N ha-1, to reduce the off-farm N losses depending on the environmental zones, land use and the production system.", "keywords": ["0301 basic medicine", "Crop productivity; DAISY model; Grain yield; Long-term experiment; Nitrogen; Pedotransfer functions; Plant available water;", "Nitrogen", "QH301 Biology", "DAISY model", "pedotransfer functions", "Plant Science", "nitrogen", "SB1-1110", "QH301", "03 medical and health sciences", "Long-term experiment", "SDG 13 - Climate Action", "Grain yield", "SDG 2 - Zero Hunger", "European Commission", "289694", "crop productivity", "SDG 15 - Life on Land", "2. Zero hunger", "020", "Pedotransfer functions", "0303 health sciences", "grain yield", "Plant culture", "15. Life on land", "plant available water", "13. Climate action", "Crop productivity", "Plant available water", "SMARTSOIL", "long-term experiment"]}, "links": [{"href": "https://flore.unifi.it/bitstream/2158/1138671/1/Ghaley%20et%20al%202018_Frontiers%20in%20Plant%20Science.pdf"}, {"href": "https://doi.org/10.3389/fpls.2018.01158"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2018.01158", "name": "item", "description": "10.3389/fpls.2018.01158", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2018.01158"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-08-08T00:00:00Z"}}, {"id": "10.3389/fpls.2018.01270", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2018-09-04", "title": "Beneficial Services of Arbuscular Mycorrhizal Fungi \u2013 From Ecology to Application", "description": "Arbuscular mycorrhiza (AM) is the most common symbiotic association of plants with microbes. AM fungi occur in the majority of natural habitats and they provide a range of important ecological services, in particular by improving plant nutrition, stress resistance and tolerance, soil structure and fertility. AM fungi also interact with most crop plants including cereals, vegetables, and fruit trees, therefore, they receive increasing attention for their potential use in sustainable agriculture. Basic research of the past decade has revealed the existence of a dedicated recognition and signaling pathway that is required for AM. Furthermore, recent evidence provided new insight into the exchange of nutritional benefits between the symbiotic partners. The great potential for application of AM has given rise to a thriving industry for AM-related products for agriculture, horticulture, and landscaping. Here, we discuss new developments in these fields, and we highlight future potential and limits toward the use of AM fungi for plant production.", "keywords": ["2. Zero hunger", "0301 basic medicine", "plant protection", "0303 health sciences", "abiotic stress", "arbuscular mycorrhiza", "Plant culture", "plant nutrition", "plant growth", "Plant Science", "15. Life on land", "symbiosis", "SB1-1110", "12. Responsible consumption", "03 medical and health sciences"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2018.01270"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2018.01270", "name": "item", "description": "10.3389/fpls.2018.01270", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2018.01270"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-04T00:00:00Z"}}, {"id": "10.3389/fpls.2019.00191", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2019-02-22", "title": "Interannual and Seasonal Dynamics of Volatile Organic Compound Fluxes From the Boreal Forest Floor", "description": "In the northern hemisphere, boreal forests are a major source of biogenic volatile organic compounds (BVOCs), which drive atmospheric processes and lead to cloud formation and changes in the Earth's radiation budget. Although forest vegetation is known to be a significant source of BVOCs, the role of soil and the forest floor, and especially interannual variations in fluxes, remains largely unknown due to a lack of long-term measurements. Our aim was to determine the interannual, seasonal and diurnal dynamics of boreal forest floor volatile organic compound (VOC) fluxes and to estimate how much they contribute to ecosystem VOC fluxes. We present here an 8-year data set of forest floor VOC fluxes, measured with three automated chambers connected to the quadrupole proton transfer reaction mass spectrometer (quadrupole PTR-MS). The exceptionally long data set shows that forest floor fluxes were dominated by monoterpenes and methanol, with relatively comparable emission rates between the years. Weekly mean monoterpene fluxes from the forest floor were highest in spring and in autumn (maximum 59 and 86 \u03bcg m-2 h-1, respectively), whereas the oxygenated VOC fluxes such as methanol had highest weekly mean fluxes in spring and summer (maximum 24 and 79 \u03bcg m-2 h-1, respectively). Although the chamber locations differed from each other in emission rates, the inter-annual dynamics were very similar and systematic. Accounting for this chamber location dependent variability, temperature and relative humidity, a mixed effects linear model was able to explain 79-88% of monoterpene, methanol, acetone, and acetaldehyde fluxes from the boreal forest floor. The boreal forest floor was a significant contributor in the forest stand fluxes, but its importance varies between seasons, being most important in autumn. The forest floor emitted 2-93% of monoterpene fluxes in spring and autumn and 1-72% of methanol fluxes in spring and early summer. The forest floor covered only a few percent of the forest stand fluxes in summer.", "keywords": ["VOC EMISSIONS", "Plant Science", "ATMOSPHERIC OH", "01 natural sciences", "forest floor", "SB1-1110", "MONOTERPENE EMISSIONS", "vegetation", "biogenic volatile organic compound", "11. Sustainability", "SCOTS PINE", "EXCHANGE", "0105 earth and related environmental sciences", "decomposition", "CLIMATE-CHANGE", "seasonality", "temperature", "Plant culture", "Forestry", "15. Life on land", "SOIL", "MODEL", "Environmental sciences", "flux", "13. Climate action", "PTR-TOF", "METHANOL"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2019.00191"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2019.00191", "name": "item", "description": "10.3389/fpls.2019.00191", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2019.00191"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-22T00:00:00Z"}}, {"id": "10.3389/fpls.2021.782072", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-12-20", "title": "Phenotyping of Different Italian Durum Wheat Varieties in Early Growth Stage With the Addition of Pure or Digestate-Activated Biochars", "description": "

This study aims to highlight the major effects of biochar incorporation into potting soil substrate on plant growth and performance in early growth stages of five elite Italian varieties of durum wheat (Triticum durum). The biochars used were obtained from two contrasting feedstocks, namely wood chips and wheat straw, by gasification under high temperature conditions, and were applied in a greenhouse experiment either as pure or as nutrient-activated biochar obtained by incubation with digestate. The results of the experiment showed that specific genotypes as well as different treatments with biochar have significant effects on plant response when looking at shoot traits related to growth. The evaluated genotypes could be clustered in two main distinct groups presenting, respectively, significantly increasing (Duilio, Iride, and Saragolla varieties) and decreasing (Marco Aurelio and Grecale varieties) values of projected shoot system area (PSSA), fresh weight (FW), dry weight (DW), and plant water loss by evapotranspiration (ET). All these traits were correlated with Pearson correlation coefficients ranging from 0.74 to 0.98. Concerning the treatment effect, a significant alteration of the mentioned plant traits was observed when applying biochar from wheat straw, characterized by very high electrical conductivity (EC), resulting in a reduction of 34.6% PSSA, 43.2% FW, 66.9% DW, and 36.0% ET, when compared to the control. Interestingly, the application of the same biochar after nutrient spiking with digestate determined about a 15\uffe2\uff80\uff9330% relief from the abovementioned reduction induced by the application of the sole pure wheat straw biochar. Our results reinforce the current basic knowledge available on biological soil amendments as biochar and digestate. remaining sound up to 6\u201312 months after harvest. Most have been selected under semiarid
Mediterranean summer conditions with poor irrigation or rain-fed and thus, are
drought tolerant. Besides the convergence in the latter traits, local selection criteria have
been very variable, leading to a wide variation in fruit morphology and quality traits. The
different soil characteristics and agricultural management techniques across the
Mediterranean denote also a wide range of plant adaptive traits to different conditions.
Despite the notorious traits for fruit quality and environment adaptation, the LSL landraces
have been poorly exploited in tomato breeding programs, which rely basically on wild
tomato species. In this review, we describe most of the information currently available for
Mediterranean LSL landraces in order to highlight the importance of this genetic resource.
We focus on the origin and diversity, the main selective traits, and the determinants of the
extended fruit shelf-life and the drought tolerance. Altogether, the Mediterranean LSL
landraces are a very valuable heritage to be revalued, since constitutes an alternative
source to improve fruit quality and shelf-life in tomato, and to breed for more resilient
cultivars under the predicted climate change conditions.", "keywords": ["0106 biological sciences", "2. Zero hunger", "Yield", "extended fruit shelf-life", "drought tolerance", "Plant culture", "Extended fruit shelf-life", "gas exchange", "Plant Science", "tomato", "Drought tolerance", "15. Life on land", "01 natural sciences", "Mediterranean landraces", "Tomato", "SB1-1110", "Fruit quality traits", "Gas exchange", "fruit quality traits"], "contacts": [{"organization": "Conesa, Miguel A., Fullana-Pericas, Mateu, GRANELL RICHART, ANTONIO, Galmes, Jeroni,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fpls.2019.01651"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2019.01651", "name": "item", "description": "10.3389/fpls.2019.01651", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2019.01651"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-10T00:00:00Z"}}, {"id": "10.3389/fpls.2021.813380", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-12-03", "title": "Corrigendum: Involvement of Arabidopsis Multi-Copper Oxidase-Encoding LACCASE12 in Root-to-Shoot Iron Partitioning: A Novel Example of Copper-Iron Crosstalk", "description": "2 Pags.- 1 Fig. \u00a9 2021 Bernal and Kr\u00e4mer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). A Corrigendum on Involvement of Arabidopsis Multi-Copper Oxidase-Encoding LACCASE12 in Root-to-Shoot Iron Partitioning: A Novel Example of Copper-Iron Crosstalk / by Bernal, M., and Kr\u00e4mer, U. (2021). Front. Plant Sci. 12:688318. doi: 10.3389/fpls.2021.688318. In the original article, there was a mistake in Figure 2B as published. The panel B showed the positive control of panel A and it was accidentally taken from a different experiment when the figure was prepared. The corrected Figure 2 with the correct positive control plate appears below. Peer reviewed", "keywords": ["iron", "multicopper oxidase", "copper", "homeostasis", "Plant culture", "deficiency", "Plant Science", "SB1-1110"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2021.813380"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2021.813380", "name": "item", "description": "10.3389/fpls.2021.813380", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2021.813380"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-03T00:00:00Z"}}, {"id": "10.3389/fpls.2020.01068", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2020-07-16", "title": "Towards Better Understanding of the Interactions and Efficient Application of Plant Beneficial Prebiotics, Probiotics, Postbiotics and Synbiotics", "description": "Project EXCALIBUR from the European Union's Horizon 2020 research and innovation programme 817946", "keywords": ["2. Zero hunger", "0301 basic medicine", "microbial metabolites", "0303 health sciences", "Microbial metabolites", "plant beneficial microorganisms", "Plant culture", "formulation", "Microbiome management", "Plant beneficial microorganisms", "Plant Science", "microbiome management", "15. Life on land", "6. Clean water", "SB1-1110", "03 medical and health sciences", "Prebiotics", "Formulation", "13. Climate action", "prebiotics"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2020.01068"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2020.01068", "name": "item", "description": "10.3389/fpls.2020.01068", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2020.01068"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-16T00:00:00Z"}}, {"id": "10.3389/fpls.2020.00270", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2020-03-10", "title": "Formulation of Microbial Inoculants by Encapsulation in Natural Polysaccharides: Focus on Beneficial Properties of Carrier Additives and Derivatives", "description": "In the last 10-15 years, the wide application of bioformulated plant beneficial microorganisms is accepted as an effective alternative of chemical agro-products. Two main problems can be distinguished in their production and application: (a) economical competiveness based on the overall up-stream and down-stream operational costs, and (b) development of commercial products with a high soil-plant colonization potential in controlled conditions but not able to effectively mobilize soil nutrients and/or combat plant pathogens in the field. To solve the above problems, microbe-based formulations produced by immobilization methods are gaining attention as they demonstrate a large number of advantages compared to other solid and liquid formulations. This mini-review summarizes the knowledge of additional compounds that form part of the bioformulations. The additives can exert economical, price-decreasing effects as bulking agents or direct effects improving microbial survival during storage and after introduction into soil with simultaneous beneficial effects on soil and plants. In some studies, combinations of additives are used with a complex impact, which improves the overall characteristics of the final products. Special attention is paid to polysaccharide carriers and their derivates, which play stimulatory role on plants but are less studied. The mini-review also focuses on the potential difficulty in evaluating the effects of complex bio-formulations.", "keywords": ["biofertilizers", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "biofertilizers", " formulation", " immobilization", " polysaccharides", " additives", "polysaccharides", "Plant culture", "formulation", "Plant Science", "Biofertilizers", "6. Clean water", "SB1-1110", "12. Responsible consumption", "Immobilization", "03 medical and health sciences", "Formulation", "Polysaccharides", "immobilization", "additives"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2020.00270"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2020.00270", "name": "item", "description": "10.3389/fpls.2020.00270", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2020.00270"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-10T00:00:00Z"}}, {"id": "10.3389/fpls.2020.00274", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2020-03-03", "title": "Editorial: Interactions of Plants With Bacteria and Fungi: Molecular and Epigenetic Plasticity of the Host", "description": "Interactions of Plants with Bacteria and Fungi:Molecular and Epigenetic Plasticity of the Host", "keywords": ["genotypes; molecular mechanisms; omics tools; plant-bacterial interactions; plant-fungal interactions", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "plant-bacterial interactions", "omics tools", "molecular mechanisms", "Plant culture", "Molecular mechanisms", "Plant Science", "SB1-1110", "Plant-fungal Interactions", "03 medical and health sciences", "plant-fungal interactions", "Plant-bacterial Interactions", "genotypes", "Omics Tools"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2020.00274"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2020.00274", "name": "item", "description": "10.3389/fpls.2020.00274", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2020.00274"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-04T00:00:00Z"}}, {"id": "10.3389/fpls.2020.00885", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2020-06-24", "title": "A Compost Treatment Acts as a Suppressive Agent in Phytophthora capsici \u2013 Cucurbita pepo Pathosystem by Modifying the Rhizosphere Microbiota", "description": "Phytophthora capsici Leonian (PHC) is a filamentous pathogen oomycete that causes root, fruit, foliar and crown rot over a wide host range, including the economically and nutritionally important summer squash (Cucurbita pepo var. cylindrica L.) crop. PHC chemical control strategies are difficult to adopt, due to the limited number of registered chemicals that are permitted and the scalar harvest system. For these reasons, other strategies, such as the use of waste-based composts that can act as suppressive agents against several soilborne pathogens, have been studied intensively. It is well known that compost's microbiota plays an important role to confer its suppressive ability. In this study, four different composts were analyzed with both 16S rRNA gene and 18S rRNA gene real-time PCR amplification and with 26S gene amplicon-based sequencing; the total abundance of the bacterial and fungal communities was found to be higher compared to literature, thus confirming that the four composts were a good inoculum source for agricultural applications. The core mycobiota was mainly composed of 31 genera; nevertheless, it was possible to observe a clear predominance of the same few taxa in all the composts. The four composts were then tested, at different concentrations (1-10-20% v/v), to establish their ability to confer suppressiveness to the Phytophthora capsici (PHC) - Cucurbita pepo pathosystem in controlled greenhouse pot trials. A total of 12 compost mixtures were considered, and of these, one (Trichoderma-enriched compost at 10% v/v) was able to statistically reduce the disease incidence caused by PHC (by 50% compared to the untreated control). Hence, the microbiota composition of the most effective compost treatment was investigated and compared with untreated and chemical (metalaxyl) controls. Mycobiota sequencing showed genera differences between the three treatments, with relative abundances of several fungal genera that were significantly different among the samples. Moreover, PCA analyses clustered the compost treatment differently from the chemical and the untreated controls. These findings suggest that suppressive activity of a compost is strictly influenced by its microbiota and the applied dosage, but the ability to induce a shaping in the rhizosphere microbial composition is also required.", "keywords": ["Trichoderma", "0301 basic medicine", "2. Zero hunger", "compost", "0303 health sciences", "Plant culture", "Plant Science", "SB1-1110", "compost; Cucurbita pepo; mycobiota; Phytophthora capsici; soil metataxonomy; Trichoderma", "Cucurbita pepo", "03 medical and health sciences", "soil metataxonomy", "Phytophthora capsici", "mycobiota"]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/1762457/1/Bellini%20et%20al%202020%20Frontiers%20in%20plant%20science.pdf"}, {"href": "https://doi.org/10.3389/fpls.2020.00885"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2020.00885", "name": "item", "description": "10.3389/fpls.2020.00885", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2020.00885"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-06-24T00:00:00Z"}}, {"id": "10.3389/fpls.2020.00889", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-04-28T16:21:04Z", "type": "Journal Article", "created": "2020-06-23", "title": "An Optimized in situ Quantification Method of Leaf H2O2 Unveils Interaction Dynamics of Pathogenic and Beneficial Bacteria in Wheat", "description": "Hydrogen peroxide (H2O2) functions as an important signaling molecule in plants during biotic interactions. However, the extent to which H2O2 accumulates during these interactions and its implications in the development of disease symptoms is unclear. In this work, we provide a step-by-step optimized protocol for in situ quantification of relative H2O2 concentrations in wheat leaves infected with the pathogenic bacterium Pseudomonas syringae pv. atrofaciens (Psa), either alone or in the presence of the beneficial bacterium Herbaspirillum seropedicae (RAM10). This protocol involved the use of 3-3'diaminobenzidine (DAB) staining method combined with image processing to conduct deconvolution and downstream analysis of the digitalized leaf image. The application of a linear regression model allowed to relate the intensity of the pixels resulting from DAB staining with a given concentration of H2O2. Decreasing H2O2 accumulation patterns were detected at increasing distances from the site of pathogen infection, and H2O2 concentrations were different depending on the bacterial combinations tested. Notably, Psa-challenged plants in presence of RAM10 accumulated less H2O2 in the leaf and showed reduced necrotic symptoms, pointing to a potential role of RAM10 in reducing pathogen-triggered H2O2 levels in young wheat plants.", "keywords": ["biotic interactions", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "color deconvolution", "hydrogen peroxide (H2O2)", "Plant culture", "Plant Science", "3-3\u2032diaminobenzidine (DAB)", "image processing", "SB1-1110"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2020.00889"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2020.00889", "name": "item", "description": "10.3389/fpls.2020.00889", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2020.00889"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-06-23T00:00:00Z"}}, {"id": "10.3389/fpls.2020.558979", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2020-10-27", "title": "Insect Herbivory Strongly Modifies Mountain Birch Volatile Emissions", "description": "Insect herbivory is known to augment emissions of biogenic volatile organic compounds (BVOCs). Yet few studies have quantified BVOC responses to insect herbivory in natural populations in pan-Arctic regions. Here, we assess how quantitative and qualitative BVOC emissions change with increasing herbivore feeding intensity in the Subarctic mountain birch (Betula pubescens var pumila (L.)) forest. We conducted three field experiments in which we manipulated the larval density of geometrid moths (Operophtera brumata and Epirrita autumnata), on branches of mountain birch and measured BVOC emissions using the branch enclosure method and gas chromatography-mass spectrometry. Our study showed that herbivory significantly increased BVOC emissions from the branches damaged by larvae. BVOC emissions increased due to insect herbivory at relatively low larvae densities, causing up to 10% of leaf area loss. Insect herbivory also changed the blend composition of BVOCs, with damaged plants producing less intercorrelated BVOC blends than undamaged ones. Our results provide a quantitative understanding of the relationship between the severity of insect herbivore damage and emissions of BVOCs at larvae densities corresponding to background herbivory levels in the Subarctic mountain birch. The results have important and practical implications for modeling induced and constitutive BVOC emissions and their feedbacks to atmospheric chemistry.", "keywords": ["0301 basic medicine", "0303 health sciences", "VDP::Zoologiske og botaniske fag: 480", "insect herbivory", "Plant culture", "Plant Science", "15. Life on land", "geometrid moth", "SB1-1110", "03 medical and health sciences", "biotic stress", "mountain birch", "stress severity", "13. Climate action", "volatile organic compounds", "11. Sustainability", "arctic", "VDP::Zoology and botany: 480"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2020.558979"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2020.558979", "name": "item", "description": "10.3389/fpls.2020.558979", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2020.558979"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-10-27T00:00:00Z"}}, {"id": "10.3389/fpls.2020.604781", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-01-12", "title": "Using Genome-Wide Predictions to Assess the Phenotypic Variation of a Barley (Hordeum sp.) Gene Bank Collection for Important Agronomic Traits and Passport Information", "description": "

Genome-wide predictions are a powerful tool for predicting trait performance. Against this backdrop we aimed to evaluate the potential and limitations of genome-wide predictions to inform the barley collection of theFederal ex situ Genebank for Agricultural and Horticultural Cropswith phenotypic data on complex traits including flowering time, plant height, thousand grain weight, as well as on growth habit and row type. We used previously published sequence data, providing information on 306,049 high-quality SNPs for 20,454 barley accessions. The prediction abilities of the two unordered categorical traits row type and growth type as well as the quantitative traits flowering time, plant height and thousand grain weight were investigated using different cross validation scenarios. Our results demonstrate that the unordered categorical traits can be predicted with high precision. In this way genome-wide prediction can be routinely deployed to extract information pertinent to the taxonomic status of gene bank accessions. In addition, the three quantitative traits were also predicted with high precision, thereby increasing the amount of information available for genotyped but not phenotyped accessions. Deeply phenotyped core collections, such as the barley 1,000 core set of the IPK Gatersleben, are a promising training population to calibrate genome-wide prediction models. Consequently, genome-wide predictions can substantially contribute to increase the attractiveness of gene bank collections and help evolve gene banks into bio-digital resource centers.

", "keywords": ["2. Zero hunger", "0301 basic medicine", "genetic resources", "0303 health sciences", "03 medical and health sciences", "bio-digital resource center", "genome-wide prediction ; gene bank genomics ; barley ; bio-digital resource center ; genetic resources", "gene bank genomics", "barley", "Plant culture", "genome-wide prediction", "Plant Science", "SB1-1110"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2020.604781"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2020.604781", "name": "item", "description": "10.3389/fpls.2020.604781", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2020.604781"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-11T00:00:00Z"}}, {"id": "10.3389/fpls.2021.608967", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-03-10", "title": "Remote Sensing Energy Balance Model for the Assessment of Crop Evapotranspiration and Water Status in an Almond Rootstock Collection", "description": "

One of the objectives of many studies conducted by breeding programs is to characterize and select rootstocks well-adapted to drought conditions. In recent years, field high-throughput phenotyping methods have been developed to characterize plant traits and to identify the most water use efficient varieties and rootstocks. However, none of these studies have been able to quantify the behavior of crop evapotranspiration in almond rootstocks under different water regimes. In this study, remote sensing phenotyping methods were used to assess the evapotranspiration of almond cv. \uffe2\uff80\uff9cMarinada\uffe2\uff80\uff9d grafted onto a rootstock collection. In particular, the two-source energy balance and Shuttleworth and Wallace models were used to, respectively, estimate the actual and potential evapotranspiration of almonds grafted onto 10 rootstock under three different irrigation treatments. For this purpose, three flights were conducted during the 2018 and 2019 growing seasons with an aircraft equipped with a thermal and multispectral camera. Stem water potential (\uffce\uffa8stem) was also measured concomitant to image acquisition. Biophysical traits of the vegetation were firstly assessed through photogrammetry techniques, spectral vegetation indices and the radiative transfer model PROSAIL. The estimates of canopy height, leaf area index and daily fraction of intercepted radiation had root mean square errors of 0.57 m, 0.24 m m\uffe2\uff80\uff931 and 0.07%, respectively. Findings of this study showed significant differences between rootstocks in all of the evaluated parameters. Cadaman\uffc2\uffae and Garnem\uffc2\uffae had the highest canopy vigor traits, evapotranspiration, \uffce\uffa8stem and kernel yield. In contrast, Rootpac\uffc2\uffae 20 and Rootpac\uffc2\uffae R had the lowest values of the same parameters, suggesting that this was due to an incompatibility between plum-almond species or to a lower water absorption capability of the rooting system. Among the rootstocks with medium canopy vigor, Adesoto and IRTA 1 had a lower evapotranspiration than Rootpac\uffc2\uffae 40 and Ishtara\uffc2\uffae. Water productivity (WP) (kg kernel/mm water evapotranspired) tended to decrease with \uffce\uffa8stem, mainly in 2018. Cadaman\uffc2\uffae and Garnem\uffc2\uffae had the highest WP, followed by INRA GF-677, IRTA 1, IRTA 2, and Rootpac\uffc2\uffae 40. Despite the low \uffce\uffa8stem of Rootpac\uffc2\uffae R, the WP of this rootstock was also high.

", "keywords": ["stem water potential", "2. Zero hunger", "Plant culture", "field phenotyping", "633", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "TSEB model", "6. Clean water", "thermal", "SB1-1110", "631", "crown area", "water productivity", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2021.608967"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2021.608967", "name": "item", "description": "10.3389/fpls.2021.608967", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2021.608967"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-10T00:00:00Z"}}, {"id": "10.3389/fpls.2021.616645", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-02-17", "title": "Microplastic Shape, Polymer Type, and Concentration Affect Soil Properties and Plant Biomass", "description": "

Microplastics may enter the soil in a wide range of shapes and polymers. However, little is known about the effects that microplastics of different shapes, polymers, and concentration may have on soil properties and plant performance. To address this, we selected 12 microplastics representing different shapes (fibers, films, foams, and fragments) and polymers, and mixed them each with soil at a concentration of 0.1, 0.2, 0.3, and 0.4%. A phytometer (Daucus carota) grew in each pot during 4 weeks. Shoot, root mass, soil aggregation, and microbial activity were measured. All shapes increased plant biomass. Shoot mass increased by \uffe2\uff88\uffbc27% with fibers, \uffe2\uff88\uffbc60% with films, \uffe2\uff88\uffbc45% with foams, and by \uffe2\uff88\uffbc54% with fragments, as fibers hold water in the soil for longer, films decrease soil bulk density, and foams and fragments can increase soil aeration and macroporosity, which overall promote plant performance. By contrast, all shapes decreased soil aggregation by \uffe2\uff88\uffbc25% as microplastics may introduce fracture points into aggregates and due to potential negative effects on soil biota. The latter may also explain the decrease in microbial activity with, for example, polyethylene films. Our findings show that shape, polymer type, and concentration are key properties when studying microplastic effects on terrestrial systems.

", "keywords": ["580", "porosity", "0211 other engineering and technologies", "Plant culture", "Plant Science", "02 engineering and technology", "15. Life on land", "01 natural sciences", "6. Clean water", "SB1-1110", "Daucus carota", "500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::580 Pflanzen (Botanik)", "soil water status", "water-stable aggregates", "microresp", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2021.616645"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2021.616645", "name": "item", "description": "10.3389/fpls.2021.616645", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2021.616645"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-16T00:00:00Z"}}, {"id": "10.3389/fpls.2021.626709", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-02-01", "title": "Potential Effects of Microplastic on Arbuscular Mycorrhizal Fungi", "description": "

Microplastics (MPs) are ubiquitously found in terrestrial ecosystems and are increasingly recognized as a factor of global change (GCF). Current research shows that MP can alter plant growth, soil inherent properties, and the composition and activity of microbial communities. However, knowledge about how microplastic affects arbuscular mycorrhizal fungi (AMF) is scarce. For plants it has been shown that microplastic can both increase and decrease the aboveground biomass and reduce the root diameter, which could indirectly cause a change in AMF abundance and activity. One of the main direct effects of microplastic is the reduction of the soil bulk density, which translates to an altered soil pore structure and water transport. Moreover, especially fibers can have considerable impacts on soil structure, namely the size distribution and stability of soil aggregates. Therefore, microplastic alters a number of soil parameters that determine habitat space and conditions for AMF. We expect that this will influence functions mediated by AMF, such as soil aggregation, water and nutrient transport. We discuss how the impacts of microplastic on AMF could alter how plants deal with other GCFs in the context of sustainable food production. The co-occurrence of several GCFs, e.g., elevated temperature, drought, pesticides, and microplastic could modify the impact of microplastic on AMF. Furthermore, the ubiquitous presence of microplastic also relates to earth system processes, e.g., net primary production (NPP), carbon and nitrogen cycling, which involve AMF as key soil organisms. For future research, we outline which experiments should be prioritized.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "570", "0303 health sciences", "Plant culture", "arbuscular mycorrhizal fungi", "Plant Science", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie", "15. Life on land", "6. Clean water", "SB1-1110", "03 medical and health sciences", "13. Climate action", "earth system processes", "pollution", "microplastic", "global change"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2021.626709"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2021.626709", "name": "item", "description": "10.3389/fpls.2021.626709", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2021.626709"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10.3389/fpls.2021.642027", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-04-09", "title": "Microbiome-Assisted Breeding to Understand Cultivar-Dependent Assembly in Cucurbita pepo", "description": "

Recently, it was shown that long-term plant breeding does not only shape plant characteristics but also impacts plant-associated microbiota substantially. This requires a microbiome-integrative breeding approach, which was not yet shown. Here we investigate this for the Styrian oil pumpkin (Cucurbita pepoL. subsp.pepovar.styriacaGreb.) by analyzing the microbiome of six genotypes (the complete pedigree of a three-way cross-hybrid, consisting of three inbred lines and one open pollinating cultivar) in the seed and rhizosphere as well as the progeny seeds. Using high-throughput amplicon sequencing targeting the 16S rRNA and the ITS1 genes, the bacterial and fungal microbiomes were accessed. Seeds were found to generally carry a significantly lower microbial diversity compared to the rhizosphere and soil as well as a different microbial composition, with an especially high fraction ofEnterobacteriaceae(40\uffe2\uff80\uff9383%). Additionally, potential plant-beneficial bacterial taxa, includingBacillaceae,Burkholderiaceae, andPseudomonadaceae, were found to be enriched in progeny seeds. Between genotypes, more substantial changes can be observed for seed microbiomes compared to the rhizosphere. Moreover, rhizosphere communities were assembled for the most part from soil. Interestingly, bacterial signatures are mainly linked from seed to seed, while fungal communities are shaped by the soil and rhizosphere. Our findings provide a deep look into the rhizosphere and seed microbiome assembly of pumpkin-associated communities and represent the first steps into microbiome-driven breeding for plant-beneficial microbes.The current lack of efficient methods for high throughput field phenotyping is a constraint on the goal of increasing durum wheat yields. This study illustrates a comprehensive methodology for phenotyping this crop's water use through the use of the two-source energy balance (TSEB) model employing very high resolution imagery. An unmanned aerial vehicle (UAV) equipped with multispectral and thermal cameras was used to phenotype 19 durum wheat cultivars grown under three contrasting irrigation treatments matching crop evapotranspiration levels (ETc): 100%ETc treatment meeting all crop water requirements (450 mm), 50%ETc treatment meeting half of them (285 mm), and a rainfed treatment (122 mm). Yield reductions of 18.3 and 48.0% were recorded in the 50%ETc and rainfed treatments, respectively, in comparison with the 100%ETc treatment. UAV flights were carried out during jointing (April 4th), anthesis (April 30th), and grain-filling (May 22nd). Remotely-sensed data were used to estimate: (1) plant height from a digital surface model (H, R2 = 0.95, RMSE = 0.18m), (2) leaf area index from multispectral vegetation indices (LAI, R2 = 0.78, RMSE = 0.63), and (3) actual evapotranspiration (ETa) and transpiration (T) through the TSEB model (R2 = 0.50, RMSE = 0.24 mm/h). Compared with ground measurements, the four traits estimated at grain-filling provided a good prediction of days from sowing to heading (DH, r = 0.58\uffe2\uff80\uff930.86), to anthesis (DA, r = 0.59\uffe2\uff80\uff930.85) and to maturity (r = 0.67\uffe2\uff80\uff930.95), grain-filling duration (GFD, r = 0.54\uffe2\uff80\uff930.74), plant height (r = 0.62\uffe2\uff80\uff930.69), number of grains per spike (NGS, r = 0.41\uffe2\uff80\uff930.64), and thousand kernel weight (TKW, r = 0.37\uffe2\uff80\uff930.42). The best trait to estimate yield, DH, DA, and GFD was ETa at anthesis or during grain filling. Better forecasts for yield-related traits were recorded in the irrigated treatments than in the rainfed one. These results show a promising perspective in the use of energy balance models for the phenotyping of large numbers of durum wheat genotypes under Mediterranean conditions.

", "keywords": ["2. Zero hunger", "grain weight", "Plant culture", "633", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "yield", "6. Clean water", "transpiration", "plant height", "SB1-1110", "631", "remote sensing", "0401 agriculture", " forestry", " and fisheries", "grain number"], "contacts": [{"organization": "G\u00f3mez-Cand\u00f3n, David, Bellvert, Joaquim, Royo, Conxita,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fpls.2021.658357"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2021.658357", "name": "item", "description": "10.3389/fpls.2021.658357", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2021.658357"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-04-16T00:00:00Z"}}, {"id": "10.3389/fpls.2021.670236", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-06-04", "title": "Impact of Plant Growth-Promoting Rhizobacteria Inoculation and Grafting on Tolerance of Tomato to Combined Water and Nutrient Stress Assessed via Metabolomics Analysis", "description": "

In the current study, inoculation with plant growth-promoting rhizobacteria (PGPR) and grafting were tested as possible cultural practices that may enhance resilience of tomato to stress induced by combined water and nutrient shortage. The roots of tomato grown on perlite were either inoculated or not with PGPR, applying four different treatments. These were PGPR-T1, a mix of two Enterobacter sp. strains (C1.2 and C1.5); PGPR-T2, Paenibacillus sp. strain DN1.2; PGPR-T3, Enterobacter mori strain C3.1; and PGPR-T4, Lelliottia sp. strain D2.4. PGPR-treated plants were either self-grafted or grafted onto Solanum lycopersicum cv. M82 and received either full or 50% of their standard water, nitrogen, and phosphorus needs. The vegetative biomass of plants subjected to PGPR-T1 was not reduced when plants were cultivated under combined stress, while it was reduced by stress to the rest of the PGPR treatments. However, PGPR-T3 increased considerably plant biomass of non-stressed tomato plants than did all other treatments. PGPR application had no impact on fruit biomass, while grafting onto \uffe2\uff80\uff99M82\uffe2\uff80\uff99 increased fruit production than did self-grafting. Metabolomics analysis in tomato leaves revealed that combined stress affects several metabolites, most of them already described as stress-related, including trehalose, myo-inositol, and monopalmitin. PGPR inoculation with E. mori strain C3.1 affected metabolites, which are important for plant/microbe symbiosis (myo-inositol and monopalmitin). The rootstock M82 did not affect many metabolites in plant leaves, but it clearly decreased the levels of malate and D-fructose and imposed an accumulation of oleic acid. In conclusion, PGPR are capable of increasing tomato tolerance to combined stress. However, further research is required to evaluate more strains and refine protocols for their application. Metabolites that were discovered as biomarkers could be used to accelerate the screening process for traits such as stress tolerance to abiotic and/or abiotic stresses. Finally, \uffe2\uff80\uff98M82\uffe2\uff80\uff99 is a suitable rootstock for tomato, as it is capable of increasing fruit biomass production.

", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "Plant culture", "hydroponics", "Plant Science", "tomato", "15. Life on land", "grafting", "metabolomics", "01 natural sciences", "6. Clean water", "SB1-1110", "water stress", "03 medical and health sciences", "PGPR", "M82", "biostimulant"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2021.670236"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2021.670236", "name": "item", "description": "10.3389/fpls.2021.670236", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2021.670236"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-04T00:00:00Z"}}, {"id": "10.3389/fpls.2022.965576", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:06Z", "type": "Journal Article", "created": "2022-08-08", "title": "Legacy effect of microplastics on plant-soil feedbacks", "description": "

Microplastics affect plants and soil biota and the processes they drive. However, the legacy effect of microplastics on plant\uffe2\uff80\uff93soil feedbacks is still unknown. To address this, we used soil conditioned from a previous experiment, where Daucus carota grew with 12 different microplastic types (conditioning phase). Here, we extracted soil inoculum from those 12 soils and grew during 4\uffe2\uff80\uff89weeks a native D. carota and a range-expanding plant species Calamagrostis epigejos in soils amended with this inoculum (feedback phase). At harvest, plant biomass and root morphological traits were measured. Films led to positive feedback on shoot mass (higher mass with inoculum from soil conditioned with microplastics than with inoculum from control soil). Films may decrease soil water content in the conditioning phase, potentially reducing the abundance of harmful soil biota, which, with films also promoting mutualist abundance, microbial activity and carbon mineralization, would positively affect plant growth in the feedback phase. Foams and fragments caused positive feedback on shoot mass likely via positive effects on soil aeration in the conditioning phase, which could have increased mutualistic biota and soil enzymatic activity, promoting plant growth. By contrast, fibers caused negative feedback on root mass as this microplastic may have increased soil water content in the conditioning phase, promoting the abundance of soil pathogens with negative consequences for root mass. Microplastics had a legacy effect on root traits: D. carota had thicker roots probably for promoting mycorrhizal associations, while C. epigejos had reduced root diameter probably for diminishing pathogenic infection. Microplastic legacy on soil can be positive or negative depending on the plant species identity and may affect plant biomass primarily via root traits. This legacy may contribute to the competitive success of range-expanding species via positive effects on root mass (foams) and on shoot mass (PET films). Overall, microplastics depending on their shape and polymer type, affect plant\uffe2\uff80\uff93soil feedbacks.

", "keywords": ["580", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "soil inocula", "Plant culture", "Plant Science", "plant\u2013soil interactions", "04 agricultural and veterinary sciences", "15. Life on land", "SB1-1110", "root morphological traits", "03 medical and health sciences", "500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::580 Pflanzen (Botanik)", "polymer type", "0401 agriculture", " forestry", " and fisheries", "microplastic shape", "plant biomass"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2022.965576"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2022.965576", "name": "item", "description": "10.3389/fpls.2022.965576", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2022.965576"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-13T00:00:00Z"}}, {"id": "10.3389/fpls.2021.682142", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:05Z", "type": "Journal Article", "created": "2021-07-21", "title": "Shifts in the Abundances of Saprotrophic and Ectomycorrhizal Fungi With Altered Leaf Litter Inputs", "description": "

Ectomycorrhizal (EcM) and saprotrophic fungi interact in the breakdown of organic matter, but the mechanisms underlying the EcM role on organic matter decomposition are not totally clear. We hypothesized that the ecological relations between EcM and saprotroph fungi are modulated by resources availability and accessibility, determining decomposition rates. We manipulated the amount of leaf litter inputs (No-Litter, Control Litter, Doubled Litter) on Trenched (root exclusion) and Non-Trenched plots (with roots) in a temperate deciduous forest of EcM-associated trees. Resultant shifts in soil fungal communities were determined by phospholipid fatty acids and DNA sequencing after 3 years, and CO2 fluxes were measured throughout this period. Different levels of leaf litter inputs generated a gradient of organic substrate availability and accessibility, altering the composition and ecological relations between EcM and saprotroph fungal communities. EcM fungi dominated at low levels of fresh organic substrates and lower organic matter quality, where short-distances exploration types seem to be better competitors, whereas saprotrophs and longer exploration types of EcM fungi tended to dominate at high levels of leaf litter inputs, where labile organic substrates were easily accessible. We were, however, not able to detect unequivocal signs of competition between these fungal groups for common resources. These results point to the relevance of substrate quality and availability as key factors determining the role of EcM and saprotroph fungi on litter and soil organic matter decay and represent a path forward on the capacity of organic matter decomposition of different exploration types of EcM fungi.Numerous central biological processes depend on the participation of the essential elements iron (Fe) or copper (Cu), including photosynthesis, respiration, cell wall remodeling and oxidative stress protection. Yet, both Fe and Cu metal cations can become toxic when accumulated in excess. Because of the potent ligand-binding and redox chemistries of these metals, there is a need for the tight and combined homeostatic control of their uptake and distribution. Several known examples pinpoint an inter-dependence of Fe and Cu homeostasis in eukaryotes, mostly in green algae, yeast and mammals, but this is less well understood in multicellular plants to date. In Arabidopsis, Cu deficiency causes secondary Fe deficiency, and this is associated with reduced in vitro ferroxidase activity and decreased root-to-shoot Fe translocation. Here we summarize the current knowledge of the cross-talk between Cu and Fe homeostasis and present a partial characterization of LACCASE12 (LAC12) that encodes a member of the multicopper oxidase (MCO) protein family in Arabidopsis. LAC12 transcript levels increase under Fe deficiency. The phenotypic characterization of two mutants carrying T-DNA insertions suggests a role of LAC12 in root-to-shoot Fe partitioning and in maintaining growth on Fe-deficient substrates. A molecular understanding of the complex interactions between Fe and Cu will be important for combating Fe deficiency in crops and for advancing biofortification approaches.Predicting species responses to climate change and land use practices requires understanding both the direct effects of environmental factors as well as the indirect effects mediated by changes in belowground and aboveground competition. Belowground root competition from surrounding vegetation and aboveground light competition are two important factors affecting seedling establishment. However, few studies have jointly examined the effect of belowground root and light competition on seedling establishment, especially under long-term nitrogen addition and mowing. Here, we examined how belowground root competition from surrounding vegetation and aboveground light competition affect seedling establishment within a long-term nitrogen addition and mowing experiment. Seedlings of two grasses (Stipa krylovii and Cleistogenes squarrosa) were grown with and without belowground root competition under control, nitrogen addition, and mowing treatments, and their growth characteristics were monitored. The seedlings of the two grasses achieved higher total biomass, height, mean shoot and root mass, but a lower root/shoot ratio in the absence than in the presence of belowground root competition. Nitrogen addition significantly decreased shoot biomass, root biomass, and the survival of the two grasses. Regression analyses revealed that the biomass of the two grass was strongly negatively correlated with net primary productivity under belowground root competition, but with the intercept photosynthetic active radiation in the absence of belowground root competition. This experiment demonstrates that belowground root competition can alter the grass seedling establishment response to light in a long-term nitrogen addition and mowing experiment.Root architectural and anatomical phenotypes are highly diverse. Specific root phenotypes can be associated with better plant growth under low nutrient and water availability. Therefore, root ideotypes have been proposed as breeding targets for more stress-resilient and resource-efficient crops. For example, root phenotypes that correspond to the Topsoil Foraging ideotype are associated with better plant growth under suboptimal phosphorus availability, and root phenotypes that correspond to the Steep, Cheap and Deep ideotype are linked to better performance under suboptimal availability of nitrogen and water. We propose that natural variation in root phenotypes translates into a diversity of different niches for microbial associations in the rhizosphere, rhizoplane and root cortex, and that microbial traits could have synergistic effects with the beneficial effect of specific root phenotypes. Oxygen and water content, carbon rhizodeposition, nutrient availability, and root surface area are all factors that are modified by root anatomy and architecture and determine the structure and function of the associated microbial communities. Recent research results indicate that root characteristics that may modify microbial communities associated with maize include aerenchyma, rooting angle, root hairs, and lateral root branching density. Therefore, the selection of root phenotypes linked to better plant growth under specific edaphic conditions should be accompanied by investigating and selecting microbial partners better adapted to each set of conditions created by the corresponding root phenotype. Microbial traits such as nitrogen transformation, phosphorus solubilization, and water retention could have synergistic effects when correctly matched with promising plant root ideotypes for improved nutrient and water capture. We propose that elucidation of the interactive effects of root phenotypes and microbial functions on plant nutrient and water uptake offers new opportunities to increase crop yields and agroecosystem sustainability.

The indiscriminate use of chemical fertilizers adversely affects ecological health and soil microbiota provoking loss of soil fertility and greater pathogen and pest presence in soil-plant systems, which further reduce the quality of food and human health. Therefore, the sustainability, circular economy, environmental safety of agricultural production, and health concerns made possible the practical realization of eco-friendly biotechnological approaches like organic matter amendments, biofertilizers, biopesticides, and reuse of agro-industrial wastes by applying novel and traditional methods and processes. However, the advancement in the field of Biotechnology/Agriculture is related to the safety of these microorganism-bearing products. While the existing regulations in this field are well-known and are applied in the preparation and application of waste organic matter and microbial inoculants, more attention should be paid to gene transfer, antibiotic resistance, contamination of the workers and environment in farms and biotech-plants, and microbiome changes. These risks should be carefully assessed, and new analytical tools and regulations should be applied to ensure safe and high-quality food and a healthy environment for people working in the field of bio-based soil amendments.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "risks of contamination", "0303 health sciences", "safety measures and regulations", "Plant culture", "pathogens", "Plant Science", "SB1-1110", "12. Responsible consumption", "3. Good health", "03 medical and health sciences", "13. Climate action", "11. Sustainability", "microbial inoculants", "organic matter"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2022.862875"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2022.862875", "name": "item", "description": "10.3389/fpls.2022.862875", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2022.862875"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-28T00:00:00Z"}}, {"id": "10.3389/fpls.2022.903661", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:06Z", "type": "Journal Article", "created": "2022-06-10", "title": "Diversity and Agronomic Performance of Lupinus mutabilis Germplasm in European and Andean Environments", "description": "

The introduction of Lupinus mutabilis (Andean lupin) in Europe will provide a new source of protein and oil for plant-based diets and biomass for bio-based products, while contributing to the improvement of marginal soils. This study evaluates for the first time the phenotypic variability of a large panel of L. mutabilis accessions both in their native environment and over two cropping conditions in Europe (winter crop in the Mediterranean region and summer crop in North-Central Europe), paving the way for the selection of accessions adapted to specific environments. The panel of 225 accessions included both germplasm pools from the Andean region and breeding lines from Europe. Notably, we reported higher grain yield in Mediterranean winter-cropping conditions (18 g/plant) than in the native region (9 g/plant). Instead, North European summer-cropping conditions appear more suitable for biomass production (up to 2 kg/plant). The phenotypic evaluation of 16 agronomical traits revealed significant variation in the panel. Principal component analyses pointed out flowering time, yield, and architecture-related traits as the main factors explaining variation between accessions. The Peruvian material stands out among the top-yielding accessions in Europe, characterized by early lines with high grain yield (e.g., LIB065, LIB072, and LIB155). Bolivian and Ecuadorian materials appear more valuable for the selection of genotypes for Andean conditions and for biomass production in Europe. We also observed that flowering time in the different environments is influenced by temperature accumulation. Within the panel, it is possible to identify both early and late genotypes, characterized by different thermal thresholds (600\uffc2\uffb0C\uffe2\uff80\uff93700\uffc2\uffb0C and 1,000\uffe2\uff80\uff931,200\uffc2\uffb0C GDD, respectively). Indications on top-yielding and early/late accessions, heritability of morpho-physiological traits, and their associations with grain yield are reported and remain largely environmental specific, underlining the importance of selecting useful genetic resources for specific environments. Altogether, these results suggest that the studied panel holds the genetic potential for the adaptation of L. mutabilis to Europe and provide the basis for initiating a breeding program based on exploiting the variation described herein.Establishment of the root-nodule symbiosis in legumes involves rhizobial infection of nodule primordia in the root cortex that is dependent on rhizobia crossing the root epidermal barrier. Two mechanisms have been described: either through root hair infection threads or through the intercellular passage of bacteria. Among the legume genera investigated, around 75% use root hair entry and around 25% the intercellular entry mode. Root-hair infection thread-mediated infection has been extensively studied in the model legumes Medicago truncatula and Lotus japonicus. In contrast, the molecular circuit recruited during intercellular infection, which is presumably an ancient and simpler pathway, remains poorly known. In recent years, important discoveries have been made to better understand the transcriptome response and the genetic components involved in legumes with obligate (Aeschynomene and Arachis spp.) and conditional (Lotus and Sesbania spp.) intercellular rhizobial infections. This review addresses these novel findings and briefly considers possible future research to shed light on the molecular players that orchestrate intercellular infection in legumes.Since soil compaction of potato fields delays shoot emergence and decreases total yield, the causes and effects of this compaction need to be better understood. In a controlled environment trial with young (before tuber initiation) plants, roots of cv. Inca Bella (a phureja group cultivar) were more sensitive to increased soil resistance (3.0 MPa) than cv. Maris Piper (a tuberosum group cultivar). Such variation was hypothesized to cause yield differences in two field trials, in which compaction treatments were applied after tuber planting. Trial 1 increased initial soil resistance from 0.15 MPa to 0.3 MPa. By the end of the growing season, soil resistance increased three-fold in the upper 20\uffc2\uffa0cm of the soil, but resistance in Maris Piper plots was up to twice that of Inca Bella plots. Maris Piper yield was 60% higher than Inca Bella and independent of soil compaction treatment, whilst compacted soil reduced Inca Bella yield by 30%. Trial 2 increased initial soil resistance from 0.2 MPa to 1.0 MPa. Soil resistance in the compacted treatments increased to similar, cultivar-dependent resistances as trial 1. Maris Piper yield was 12% higher than Inca Bella, but cultivar variation in yield response to compacted soil did not occur. Soil water content, root growth and tuber growth were measured to determine whether these factors could explain cultivar differences in soil resistance. Soil water content was similar between cultivars, thus did not cause soil resistance to vary between cultivars. Root density was insufficient to cause observed increases soil resistance. Finally, differences in soil resistance between cultivars became significant during tuber initiation, and became more pronounced until harvest. Increased tuber biomass volume (yield) of Maris Piper increased estimated mean soil density (and thus soil resistance) more than Inca Bella. This increase seems to depend on initial compaction, as soil resistance did not significantly increase in uncompacted soil. While increased soil resistance caused cultivar-dependent restriction of root density of young plants that was consistent with cultivar variation in yield, tuber growth likely caused cultivar-dependent increases in soil resistance in field trials, which may have further limited Inca Bella yield.

Global agricultural production must double by 2050 to meet the demands of an increasing world human population but this challenge is further exacerbated by climate change. Environmental stress, heat, and drought are key drivers in food security and strongly impacts on crop productivity. Moreover, global warming is threatening the survival of many species including those which we rely on for food production, forcing migration of cultivation areas with further impoverishing of the environment and of the genetic variability of crop species with fall out effects on food security. This review considers the relationship of climatic changes and their bearing on sustainability of natural and agricultural ecosystems, as well as the role of omics-technologies, genomics, proteomics, metabolomics, phenomics and ionomics. The use of resource saving technologies such as precision agriculture and new fertilization technologies are discussed with a focus on their use in breeding plants with higher tolerance and adaptability and as mitigation tools for global warming and climate changes. Nevertheless, plants are exposed to multiple stresses. This study lays the basis for the proposition of a novel research paradigm which is referred to a holistic approach and that went beyond the exclusive concept of crop yield, but that included sustainability, socio-economic impacts of production, commercialization, and agroecosystem management.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "omic", "Plant culture", "food security", "Plant Science", "15. Life on land", "global warming", "sustainability", "630", "omics", "SB1-1110", "03 medical and health sciences", "food security", " global change", " global warming", " holistic approach", " omics", " sustainability", "13. Climate action", "holistic approach", "global change"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/517112/1/Plant%20responses%20to%20climate%20change%2c%20how%20global%20warming%20may%20impact%20on%20food%20security%3a%20a%20critical%20review.pdf"}, {"href": "https://doi.org/10.3389/fpls.2023.1297569"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2023.1297569", "name": "item", "description": "10.3389/fpls.2023.1297569", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2023.1297569"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-05T00:00:00Z"}}, {"id": "10.3389/fpls.2023.1326766", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:06Z", "type": "Journal Article", "created": "2024-01-05", "title": "A collection of novel Lotus japonicus LORE1 mutants perturbed in the nodulation program induced by the Agrobacterium pusense strain IRBG74", "description": "

The Lotus japonicus population carrying new Lotus retrotransposon 1 (LORE1) insertions represents a valuable biological resource for genetic research. New insertions were generated by activation of the endogenous retroelement LORE1a in the germline of the G329-3 plant line and arranged in a 2-D system for reverse genetics. LORE1 mutants identified in this collection contributes substantially to characterize candidate genes involved in symbiotic association of L. japonicus with its cognate symbiont, the nitrogen-fixing bacteria Mesorhizobium loti that infects root nodules intracellularly. In this study we aimed to identify novel players in the poorly explored intercellular infection induced by Agrobacterium pusense IRBG74 sp. For this purpose, a forward screen of > 200,000 LORE1 seedlings, obtained from bulk propagation of G329-3 plants, inoculated with IRBG74 was performed. Plants with perturbed nodulation were scored and the offspring were further tested on plates to confirm the symbiotic phenotype. A total of 110 Lotus mutants with impaired nodulation after inoculation with IRBG74 were obtained. A comparative analysis of nodulation kinetics in a subset of 20 mutants showed that most of the lines were predominantly affected in nodulation by IRBG74. Interestingly, additional defects in the main root growth were observed in some mutant lines. Sequencing of LORE1 flanking regions in 47 mutants revealed that 92 Lotus genes were disrupted by novel LORE1 insertions in these lines. In the IM-S34 mutant, one of the insertions was located in the 5\uffc2\uffb4UTR of the LotjaGi5g1v0179800 gene, which encodes the AUTOPHAGY9 protein. Additional mutant alleles, named atg9-2 and atg9-3, were obtained in the reverse genetic collection. Nodule formation was significantly reduced in these mutant alleles after M. loti and IRBG74 inoculation, confirming the effectiveness of the mutant screening. This study describes an effective forward genetic approach to obtain novel mutants in Lotus with a phenotype of interest and to identify the causative gene(s).Flavonoids are among the main plant root exudation components, and, in addition to their role in symbiosis, they can broadly affect the functionality of plant-associated microbes: in polluted environments, for instance, flavonoids can induce the expression of the enzymatic degradative machinery to clean-up soils from xenobiotics like polychlorinated biphenyls (PCBs). However, their involvement in root community recruitment and assembly involving non-symbiotic beneficial interactions remains understudied and may be crucial to sustain the holobiont fitness under PCB stress.

Methods

By using a set of model pure flavonoid molecules and a natural blend of root exudates (REs) with altered flavonoid composition produced by Arabidopsis mutant lines affected in flavonoid biosynthesis and abundance (null mutant tt4, flavonoid aglycones hyperproducer tt8, and flavonoid conjugates hyperaccumulator ttg), we investigated flavonoid contribution in stimulating rhizocompetence traits and the catabolic potential of the model bacterial strain for PCB degradation Paraburkholderia xenovorans LB400.

Results

Flavonoids influenced the traits involved in bacterial recruitment in the rhizoplane by improving chemotaxis and motility responses, by increasing biofilm formation and by promoting the growth and activation of the PCB-degradative pathway of strain LB400, being thus potentially exploited as carbon sources, stimulating factors and chemoattractant molecules. Indeed, early rhizoplane colonization was favored in plantlets of the tt8 Arabidopsis mutant and reduced in the ttg line. Bacterial growth was promoted by the REs of mutant lines tt4 and tt8 under control conditions and reduced upon PCB-18 stress, showing no significant differences compared with the WT and ttg, indicating that unidentified plant metabolites could be involved. PCB stress presumably altered the Arabidopsis root exudation profile, although a sudden \uffe2\uff80\uff9ccry-for-help\uffe2\uff80\uff9d response to recruit strain LB400 was excluded and flavonoids appeared not to be the main determinants. In the in vitro plant\uffe2\uff80\uff93microbe interaction assays, plant growth promotion and PCB resistance promoted by strain LB400 seemed to act through flavonoid-independent mechanisms without altering bacterial colonization efficiency and root adhesion pattern.

Discussions

This study further contributes to elucidate the vast array of functions provided by flavonoids in orchestrating the early events of PCB-degrading strain LB400 recruitment in the rhizosphere and to support the holobiont fitness by stimulating the catabolic machinery involved in xenobiotics decomposition and removal.The importance of plant rhizodeposition to sustain microbial growth and induce xenobiotic degradation in polluted environments is increasingly recognized.

Methods

Here the \uffe2\uff80\uff9ccry-for-help\uffe2\uff80\uff9d hypothesis, consisting in root chemistry remodeling upon stress, was investigated in the presence of polychlorinated biphenyls (PCBs), highly recalcitrant and phytotoxic compounds, highlighting its role in reshaping the nutritional and signaling features of the root niche to accommodate PCB-degrading microorganisms.

Results

Arabidopsis exposure to 70 \uffc2\uffb5M PCB-18 triggered plant-detrimental effects, stress-related traits, and PCB-responsive gene expression, reproducing PCB phytotoxicity. The root exudates of plantlets exposed for 2 days to the pollutant were collected and characterized through untargeted metabolomics analysis by liquid chromatography\uffe2\uff80\uff93mass spectrometry. Principal component analysis disclosed a different root exudation fingerprint in PCB-18-exposed plants, potentially contributing to the \uffe2\uff80\uff9ccry-for-help\uffe2\uff80\uff9d event. To investigate this aspect, the five compounds identified in the exudate metabolomic analysis (i.e., scopoletin, N-hydroxyethyl-\uffce\uffb2-alanine, hypoxanthine, L-arginyl-L-valine, and L-seryl-L-phenylalanine) were assayed for their influence on the physiology and functionality of the PCB-degrading strains Pseudomonas alcaliphila JAB1, Paraburkholderia xenovorans LB400, and Acinetobacter calcoaceticus P320. Scopoletin, whose relative abundance decreased in PCB-18-stressed plant exudates, hampered the growth and proliferation of strains JAB1 and P320, presumably due to its antimicrobial activity, and reduced the beneficial effect of Acinetobacter P320, which showed a higher degree of growth promotion in the scopoletin-depleted mutant f6\uffe2\uff80\uff99h1 compared to Arabidopsis WT plants exposed to PCB. Nevertheless, scopoletin induced the expression of the bph catabolic operon in strains JAB1 and LB400. The primary metabolites hypoxanthine, L-arginyl-L-valine, and L-seryl-L-phenylalanine, which increased in relative abundance upon PCB-18 stress, were preferentially used as nutrients and growth-stimulating factors by the three degrading strains and showed a variable ability to affect rhizocompetence traits like motility and biofilm formation.

Discussion

These findings expand the knowledge on PCB-triggered \uffe2\uff80\uff9ccry-for-help\uffe2\uff80\uff9d and its role in steering the PCB-degrading microbiome to boost the holobiont fitness in polluted environments.

High rates of fertilizer applications potentially have significant environmental consequences, such as soil and water contamination and biodiversity loss. This study aimed to compare the use of biofertilizers and inorganic fertilizers in a broccoli crop to determine their impact on soil microorganism abundance, microbial community structure, functional gene diversity, yield, and greenhouse gas emissions. Four different fertilization treatments were designed: (i) inorganic fertilizers applied at a rate to cover the nutritional demands of the crop (F100); (ii) 50% of the rate of inorganic fertilizers added in F100 (F50); (iii) F50 + the application of a formulation of various bacteria (BA); and (iv) F50 + the application of a formulation of bacteria and non-mycorrhizal fungi (BA + FU). The results showed that reduced fertilization and the addition of both biofertilizer products had no significant effect on soil nutrients, microbial population, microbial activity, or yield when compared to conventional inorganic fertilization. Thus, microbial inoculants were ineffective in enhancing soil microbial abundance and activity, and there were no changes in GHG emissions or crop yields. Nonetheless, crop yield was positively related to total soil N, microbial activity, and CO2 emissions, confirming the positive effect of soil biodiversity on production. The application of biofertilizers can help reduce mineral fertilization in a broccoli crop with no negative effect on yield.

", "keywords": ["CO2", "Brassica oleracea var italica Plenck", "PLFAs", "Biofertilizers", "N2O", "CH4", "01 natural sciences", "SB1-1110", "12. Responsible consumption", "11. Sustainability", "Enzyme activities", "0105 earth and related environmental sciences", "biofertilizers", "2. Zero hunger", "CH4", "N2O", "Plant culture", "Nutrients", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Edafolog\u00eda y Qu\u00edmica Agr\u00edcola", "enzyme activities", "13. Climate action", "3101.02 Fabricaci\u00f3n de Abonos", "0401 agriculture", " forestry", " and fisheries", "CO2"]}, "links": [{"href": "https://doi.org/10.3390/horticulturae10010042"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Horticulturae", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/horticulturae10010042", "name": "item", "description": "10.3390/horticulturae10010042", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/horticulturae10010042"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-31T00:00:00Z"}}, {"id": "10.3390/horticulturae11020213", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:16Z", "type": "Journal Article", "created": "2025-02-17", "title": "Ecological Pea Production in Hungary: Integrating Conservation Tillage with the Application of Rhizobium spp., Ensifer spp., Pseudomonas spp., and Bacillus spp. Bacterial Inoculants for Sustainable Farming", "description": "

This study examines the impact of agroecological practices on soil quality and crop yields in small-scale farming, focusing on the combination of microbial soil inoculation, crop rotation, and conservation tillage methods. Conducted at the SZIA Agroecological Garden MATE in G\u00f6d\u00f6ll\u0151, Hungary, the experiment used 12 plots, employing various conservation tillage techniques, including soil loosening with and without microbial inoculants, as well as no-till systems with and without inoculation. Six of the plots were inoculated with beneficial bacteria to enhance nitrogen fixation, phosphorus mobility, nutrient solubilization, phytohormone production, and pathogen suppression. In 2024, peas (Pisum sativum L.) were planted following potatoes in a small-scale market-oriented crop rotation, with the continuous monitoring of crop performance and soil characteristics. This ongoing study focuses on evaluating the long-term effects of crop rotation on key agricultural parameters, aiming to optimize practices over time. Statistical analysis (one-way ANOVA) revealed no significant differences across most parameters (p > 0.05), except for total sugar content (p < 0.05), which aligns with expectations given the limited tillage prior to the study. The standard significance level of p < 0.05 was used to balance error risks, ensure adequate statistical power, and maintain consistency with established agricultural research practices. However, the study trends indicated potential long-term benefits, particularly in plots with microbial inoculants, where pea yield and pod size showed improvement compared to non-inoculated and control plots. Microbial inoculants may show long-term effects, as they gradually improve soil health, support microbial communities, and enhance nutrient cycling, which takes time to become noticeable. These findings highlight the potential advantages of combining conservation tillage with microbial inoculants, suggesting that this combination could foster enhanced soil health and productivity over time. The novel setting of this study underscores the importance of long-term monitoring to fully capture the benefits of agroecological interventions, emphasizing their role in achieving sustainable agricultural practices and improving small-scale farming outcomes.

", "keywords": ["biofertilizers", "agroecology", "no-till", "plant growth-promoting rhizobacpromoteteria", "conservation tillage", "Plant culture", "soil biology", "SB1-1110"]}, "links": [{"href": "https://doi.org/10.3390/horticulturae11020213"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Horticulturae", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/horticulturae11020213", "name": "item", "description": "10.3390/horticulturae11020213", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/horticulturae11020213"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-17T00:00:00Z"}}, {"id": "10.3390/horticulturae7120561", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:16Z", "type": "Journal Article", "created": "2021-12-10", "title": "Brown Marmorated Stink Bug (Halyomorpha halys St\u00e5l.) Attack Induces a Metabolic Response in Strawberry (Fragaria \u00d7 ananassa Duch.) Fruit", "description": "

The polyphagous brown marmorated stink bug (Halyomorpha halys St\u00e5l.) is an important pest in many countries. Recently it was noticed that it can feed on and cause damage to strawberries (Fragaria \u00d7 ananassa Duch.). The metabolic response of strawberries to brown marmorated stink bug attacks was studied. Brown marmorated stink bugs attacked strawberry fruit which had 18% lower total sugar content compared to that of the control treatment. However, organic acid content had no significant difference among the three treatments, with the exception of shikimic acid, which had the highest content in the attacked fruit. Thirty-one phenolic compounds were identified. Results showed a strong effect on secondary metabolites due to H. halys attacks. Halyomorpha halys treatment had 27% higher total analyzed phenolic content compared to the indirect Halyomorpha halys treatment. The brown marmorated stink bug significantly increased total ellagic acid derivatives (33.1% to 37% higher), hydroxycinnamic acids (22.3% higher) and anthocyanins\u2019 (39% higher) contents. Fruit attacked by Halyomorpha halys also had higher catechin and epicatechin content than that of the control treatments. This pest had a significant influence on the plant\u2019s secondary metabolism, and this improved our understanding of how a strawberry plant reacts to the attacks of this very important pest. H. halys-infested fruit are not suitable for commercial production, due to the production of off-flavors.

", "keywords": ["0106 biological sciences", "2. Zero hunger", "sladkorji", "derivati elagi\u010dne kisline", "hidroksicimetne kisline", "ellagic acid derivatives", "Plant culture", "jagode", "organske kisline", "hydroxycinnamic acids", "anthocyanins", "01 natural sciences", "SB1-1110", "3. Good health", "total organic acids", "antocianini", "info:eu-repo/classification/udc/632", "total sugars", "anthocyanins; ellagic acid derivatives; hydroxycinnamic acids; strawberry; total sugars; total organic acids", "strawberry", "Fragaria \u00d7 ananassa Duch.", "Halyomorpha halys St\u00e5l."]}, "links": [{"href": "http://www.mdpi.com/2311-7524/7/12/561/pdf"}, {"href": "https://doi.org/10.3390/horticulturae7120561"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Horticulturae", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/horticulturae7120561", "name": "item", "description": "10.3390/horticulturae7120561", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/horticulturae7120561"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-08T00:00:00Z"}}, {"id": "10.3390/horticulturae8040307", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-28T16:21:16Z", "type": "Journal Article", "created": "2022-04-05", "title": "Root-Zone Amendments of Biochar-Based Fertilizers: Yield Increases of White Cabbage in Temperate Climate", "description": "

The use of biochar is an important tool to improve soil fertility, reduce the negative environmental impacts of agriculture, and build up terrestrial carbon sinks. However, crop yield increases by biochar amendment were not shown consistently for fertile soils under temperate climate. Recent studies show that biochar is more likely to increase crop yields when applied in combination with nutrients to prepare biochar-based fertilizers. Here, we focused on the root-zone amendment of biochar combined with mineral fertilizers in a greenhouse trial with white cabbage (Brassica oleracea convar. Capitata var. Alba) cultivated in a nutrient-rich silt loam soil originating from the temperate climate zone (Bavaria, Germany). Biochar was applied at a low dosage (1.3 t ha\uffe2\uff88\uff921). The biochar was placed either as a concentrated hotspot below the seedling or it was mixed into the soil in the root zone representing a mixture of biochar and soil in the planting basin. The nitrogen fertilizer (ammonium nitrate or urea) was either applied on the soil surface or loaded onto the biochar representing a nitrogen-enhanced biochar. On average, a 12% yield increase in dry cabbage heads was achieved with biochar plus fertilizer compared to the fertilized control without biochar. Most consistent positive yield responses were observed with a hotspot root-zone application of nitrogen-enhanced biochar, showing a maximum 21% dry cabbage-head yield increase. Belowground biomass and root-architecture suggested a decrease in the fine root content in these treatments compared to treatments without biochar and with soil-mixed biochar. We conclude that the hotspot amendment of a nitrogen-enhanced biochar in the root zone can optimize the growth of white cabbage by providing a nutrient depot in close proximity to the plant, enabling efficient nutrient supply. The amendment of low doses in the root zone of annual crops could become an economically interesting application option for biochar in the temperate climate zone.

", "keywords": ["2. Zero hunger", "pyrogenic carbon capture and storage", "Plant culture", "04 agricultural and veterinary sciences", "root architecture", "15. Life on land", "PyCCS", "630", "PyCCS; pyrogenic carbon capture and storage; nitrogen fertilizer; root architecture; Shovelomics", "6. Clean water", "SB1-1110", "Shovelomics", "0401 agriculture", " forestry", " and fisheries", "nitrogen fertilizer"]}, "links": [{"href": "http://www.mdpi.com/2311-7524/8/4/307/pdf"}, {"href": "https://www.mdpi.com/2311-7524/8/4/307/pdf"}, {"href": "https://doi.org/10.3390/horticulturae8040307"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Horticulturae", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/horticulturae8040307", "name": "item", "description": "10.3390/horticulturae8040307", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/horticulturae8040307"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-05T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=SB1-1110&offset=50&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=SB1-1110&offset=50&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "prev", "title": "items (prev)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=SB1-1110&offset=0", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=SB1-1110&offset=100", "hreflang": "en-US"}], "numberMatched": 154, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-04-29T05:44:43.903371Z"}