Sustainability transitions in agriculture are explored through an analysis of niche initiatives within a common production system, relying on sustainable transitions, multi-level perspectives, and agroecological frameworks, and involving multi-actor, agricultural knowledge, and innovation systems (AKIS). The article focuses on how experimental niches and sustainable activities affect farmers\u2019 relationships with nature, and the reconceptualisation of the production system in which they operate, particularly where this system is embedded in less sustainable conventional or dominant regimes and landscapes. The need for fundamental changes, in the way that humans interact with nature, is widely argued for in order to achieve sustainable development, and farmers occupy a central role through participation in complex networks of agri-food systems. They have also found themselves disconnected from nature through conventional agri-industrial production practices. Four niches (biological control, ecological restoration, soil health, and ecological pond management) within the greenhouse sector of Almeria (SE Spain) are explored in a case study. Our results indicate that a farmer\u2019s interaction with nature is functional, but through agroecological practices, a deeper understanding of the ecosystems in which greenhouse landscapes are embedded may be gained. As they become more connected to nature and benefit from ecosystem services, they can transition to more sustainable agricultural systems.