Andean lupin in Europe has regained interest in recent years due to its high seed protein and oil content and its potential use for food, feed as well as a crop used in biorefineries. A search for suitable germplasm in combination with a determination of appropriate agronomical practices such as planting date are needed for commercial farming in new areas. The current study aims to evaluate the performance of six selected L. mutabilis accessions in a Mediterranean area using two different planting dates for two consecutive experimental years. A split plot design with accessions as subplots and planting dates as main plots was used. Measurements such as days to flowering, plant height, total number of pods and seeds per plant, seed yield and seed crude protein content were taken. All accessions performed better when cultivation started not later than the end of autumn since high temperatures occurred during late spring shortened the growth cycle and reduced yield. Earlier cultivation applied, in the middle of autumn, did not enhance significantly seed yield production. Among the accessions, LIB223 was characterized by the highest seed crude protein content in the early planting treatment, while accessions with prolonged growth cycle (LIB209, LIB214 and LIB223) produced higher seed yield than other accessions and could be further studied as promising breeding material for cultivation under the edaphoclimatic conditions tested.