{"type": "FeatureCollection", "features": [{"id": "10.1007/s13762-019-02264-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:20Z", "type": "Journal Article", "created": "2019-02-12", "title": "High-solids anaerobic digestion requires a trade-off between total solids, inoculum-to-substrate ratio and ammonia inhibition", "description": "Increasing total solids in anaerobic digestion can reduce the methane yield by highly complex bio-physical\u2013chemical mechanisms. Therefore, understanding those mechanisms and their main drivers becomes crucial to optimize this waste treatment biotechnology. In this study, seven batch experiments were conducted to investigate the effects of increasing the initial total solids in high-solids anaerobic digestion of the organic fraction of municipal solid waste. With inoculum-to-substrate ratio\u2009=\u20091.5 g VS/g VS and maximum total solids \u2264\u200919.6%, mono-digestion of the organic fraction of municipal solid waste showed a methane yield\u2009=\u2009174\u2013236 NmL CH4/g VS. With inoculum-to-substrate ratio \u2264\u20091.0 g VS/g VS and maximum total solids \u2265\u200924.0%, mono-digestion experiments acidified. Co-digestion of the organic fraction of municipal solid waste and beech sawdust permitted to reduce the inoculum-to-substrate ratio to 0.16 g VS/g VS while increasing total solids up to 30.2%, though achieving a lower methane yield (117\u2013156 NmL CH4/g VS). At each inoculum-to-substrate ratio, higher total solids corresponded to higher ammonia and volatile fatty acid accumulation. Thus, a 40% lower methane yield for mono-digestion was observed at a NH3 concentration \u2265\u20092.3 g N\u2013NH3/kg reactor content and total solids\u2009=\u200915.0%. Meanwhile, co-digestion lowered the nitrogen content, being the risk of acidification exacerbated only at total solids \u2265\u200920.0%. Therefore, the biodegradability of the substrate, as well as the operational total solids and inoculum-to-substrate ratio, are closely interrelated parameters determining the success of methanogenesis, but also the risk of ammonia inhibition in high-solids anaerobic digestion.", "keywords": ["Environmental Engineering", "[SDE.IE]Environmental Sciences/Environmental Engineering", "Organic fraction of municipal solid waste", "0211 other engineering and technologies", "500", "High-solids anaerobic digestion", "02 engineering and technology", "Co-digestion", "01 natural sciences", "7. Clean energy", "6. Clean water", "12. Responsible consumption", "Agricultural and Biological Sciences (all)", "Thermophilic", "13. Climate action", "Batch experiments", "11. Sustainability", "Environmental Chemistry", "Volatile fatty acids", "[SDE.IE] Environmental Sciences/Environmental Engineering", "Batch experiments; Co-digestion; High-solids anaerobic digestion; Methane yield; Organic fraction of municipal solid waste; Thermophilic; Volatile fatty acids; Environmental Engineering; Environmental Chemistry; Agricultural and Biological Sciences (all)", "Methane yield", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/743139/1/Batch%20Manuscript%20last%20for%20IRIS.pdf"}, {"href": "http://link.springer.com/content/pdf/10.1007/s13762-019-02264-z.pdf"}, {"href": "https://hal.inrae.fr/hal-02961893/file/Batch%20Manuscript_revf.pdf"}, {"href": "https://doi.org/10.1007/s13762-019-02264-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Environmental%20Science%20and%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s13762-019-02264-z", "name": "item", "description": "10.1007/s13762-019-02264-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s13762-019-02264-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-12T00:00:00Z"}}, {"id": "10.1016/j.agee.2013.09.013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:30Z", "type": "Journal Article", "created": "2013-10-29", "title": "Effects Of Grass-Clover Management And Cover Crops On Nitrogen Cycling And Nitrous Oxide Emissions In A Stockless Organic Crop Rotation", "description": "Nitrogen (N) supply in stockless organic farming may be improved through use of grass-clover for anaerobic digestion, producing biogas and digested manure for use as fertilizer in the crop rotation. We studied the effects of grass-clover management on N cycling, nitrous oxide (N2O) emissions and cash-crop yields in an organic arable crop rotation on a sandy loam soil in a cool temperate climate. The four-course crop rotation included spring barley (with undersown grass-clover), grass-clover, potato and winter wheat (with undersown cover crop). Two fertilization treatments were compared: \u201c\u2212M\u201d where plant material from grass-clover cuts was left in the field to decompose and no fertilizer or manure was applied to any crop in the rotation; and \u201c+M\u201d where plant material from grass-clover cuts was harvested and equivalent amounts of N in digested manure used for fertilization of cash crops in the rotation (spring barley, potato and winter wheat); actual digestion of grass-clover cuttings was not possible, instead digested pig manure was used as substitute for digested grass-clover. Nitrous oxide fluxes were monitored between April 2008 and May 2009. In general, application of digested manure had little or no effect on N2O emissions. Periods of high N2O emissions coincided with cover crop and grass-clover residue turnover, with little added effect of digested manure application. Annual N2O emissions did not vary between fertilization treatments, but the +M treatment had cash crop dry matter yields that were 14% higher than in the \u2212M treatment, and cash crop N yields were increased by 40%. The results show that reallocation of nutrients from grass-clover to cash crops following anaerobic digestion can help solve problems with low N availability. However, issues remain regarding N2O from cover crops and grass-clover in spring.", "keywords": ["anaerobic digestion", "2. Zero hunger", "nitrous oxide", "organic farming", "13. Climate action", "nitrate leaching", "grass-clover", "0401 agriculture", " forestry", " and fisheries", "cover crop", "04 agricultural and veterinary sciences", "15. Life on land", "7. Clean energy", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2013.09.013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2013.09.013", "name": "item", "description": "10.1016/j.agee.2013.09.013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2013.09.013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-12-01T00:00:00Z"}}, {"id": "10.1016/j.biortech.2018.06.099", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:50Z", "type": "Journal Article", "created": "2018-07-03", "title": "ADM1 based mathematical model of trace element precipitation/dissolution in anaerobic digestion processes", "description": "Due to the complex biogeochemistry of trace elements (TEs, e.g. Fe, Ni and Co) in anaerobic digestion processes, their role and fate is poorly understood. Challenging, time consuming and low detection limits of analytical procedures necessitate recruitment of mathematical models. A dynamic mathematical model based on anaerobic digestion model no.1 (ADM1) has been proposed to simulate the effect of TEs. New chemical equilibrium association/dissociation and precipitation/dissolution reactions have been implemented to determine TE bioavailability and their effect on anaerobic digestion. The model considers interactions with inorganic carbonate (HCO3- and CO32-), phosphate (PO43-, HPO42-, H2PO4-) and sulfide (HS- and S2-). The effect of deficiency, activation, inhibition and toxicity of TEs on the biochemical processes has been modelled based on a dose-response type inhibition function. The new model can predict: the dynamics of TEs (among carbonate, sulfide and phosphate); the starvation of TEs; and the effect of initial sulfur-phosphorus ratio in an in-silico batch anaerobic system.", "keywords": ["ADM1", "Trace elements", "0211 other engineering and technologies", "Phosphorus", "Precipitation", "02 engineering and technology", "Models", " Theoretical", "01 natural sciences", "7. Clean energy", "Trace Elements", "Bioreactors", "Solubility", "Anaerobic digestion", "Anaerobic digestion; Trace elements; Mathematical modeling; Precipitation; ADM1", "Mathematical modeling", "Anaerobiosis", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2018.06.099"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2018.06.099", "name": "item", "description": "10.1016/j.biortech.2018.06.099", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2018.06.099"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-11-01T00:00:00Z"}}, {"id": "10.1016/j.biortech.2018.12.064", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:50Z", "type": "Journal Article", "created": "2018-12-22", "title": "ADM1 based mathematical model of trace element complexation in anaerobic digestion processes", "description": "In this study, a new model based on anaerobic digestion model no.1 (ADM1) approach has been proposed to simulate trace elements (TEs) complexation, precipitation and their effect on the anaerobic batch methane production. TEs complexation reactions with VFAs and EDTA have been incorporated in an extended ADM1 model which considers TE precipitation/dissolution reactions as well as biodegradation processes. The kinetic model tracks the dynamics of 90 state variables which constitute the components of the proposed anaerobic digestion (AD) model. The incorporation of the complexation reactions required the definition of new inorganic components (EDTA species) and new complexation process rates in the ADM1 framework. The charge balance was modified accordingly to consider the effects of the additional components. The new model is able to predict: a) the effect of TE-EDTA/VFA complexation on methane production, and b) the effect of the initial calcium and magnesium concentrations on process performance.", "keywords": ["ADM1", "Trace elements", "0211 other engineering and technologies", "02 engineering and technology", "Models", " Theoretical", "Fatty Acids", " Volatile", "01 natural sciences", "7. Clean energy", "Trace Elements", "[SDU] Sciences of the Universe [physics]", "Kinetics", "Biodegradation", " Environmental", "Bioreactors", "13. Climate action", "Anaerobic digestion", "[SDU.STU] Sciences of the Universe [physics]/Earth Sciences", "Complexation", "Mathematical modeling", "Anaerobic digestion; Trace elements; Complexation; Mathematical modeling; ADM1;", "Anaerobiosis", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2018.12.064"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2018.12.064", "name": "item", "description": "10.1016/j.biortech.2018.12.064", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2018.12.064"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-03-01T00:00:00Z"}}, {"id": "10.1016/j.jenvman.2018.10.002", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:38Z", "type": "Journal Article", "created": "2018-10-05", "title": "Semi-continuous mono-digestion of OFMSW and Co-digestion of OFMSW with beech sawdust: Assessment of the maximum operational total solid content", "description": "In this study, mono-digestion of the organic fraction of municipal solid waste (OFMSW) and co-digestion of OFMSW with beech sawdust, simulating green waste, were used to investigate the maximum operational total solid (TS) content in semi-continuous high-solids anaerobic digestion (HS-AD). To alleviate substrate overloading in HS-AD, the effluent mass was relatively reduced compared to the influent mass, extending the mass retention time. To this aim, the reactor mass was daily evaluated, permitting to assess the reactor content removal by biogas production. During mono-digestion of OFMSW, the NH3 inhibition and the rapid TS removal prevented to maintain HS-AD conditions (i.e. TS\u202f\u2265\u202f10%), without exacerbating the risk of reactor acidification. In contrast, the inclusion of sawdust in OFMSW permitted to operate HS-AD up to 30% TS, before acidification occurred. Therefore, including a lignocellulosic substrate in OFMSW can prevent acidification and stabilize HS-AD at very high TS contents (i.e. 20-30%).", "keywords": ["[SDE] Environmental Sciences", "[SDV]Life Sciences [q-bio]", "substrate overloading", "Substrate overloading", "High-solids anaerobic digestion", "02 engineering and technology", "Solid Waste", "Influent/effluent uncoupling", "01 natural sciences", "7. Clean energy", "influent/effluent uncoupling", "12. Responsible consumption", "Acidification", "high-solids anaerobic digestion", "acidification", "Bioreactors", "11. Sustainability", "Fagus", "0202 electrical engineering", " electronic engineering", " information engineering", "Anaerobiosis", "ammonia inhibition", "Acidification; Ammonia inhibition; High-solids anaerobic digestion; Influent/effluent uncoupling; Substrate overloading", "0105 earth and related environmental sciences", "Ammonia inhibition", "660", "6. Clean water", "Refuse Disposal", "[SDV] Life Sciences [q-bio]", "13. Climate action", "[SDE]Environmental Sciences", "Methane"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/726869/1/Resubmission%202018_09_07.pdf"}, {"href": "https://hal.inrae.fr/hal-02623440/file/Vicente_Pastor_OFMSW_JEMA-D-18-02234R1.pdf"}, {"href": "https://doi.org/10.1016/j.jenvman.2018.10.002"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jenvman.2018.10.002", "name": "item", "description": "10.1016/j.jenvman.2018.10.002", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jenvman.2018.10.002"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-01T00:00:00Z"}}, {"id": "10.1016/j.jenvman.2019.03.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:38Z", "type": "Journal Article", "created": "2019-03-11", "title": "Modelling non-ideal bio-physical-chemical effects on high-solids anaerobic digestion of the organic fraction of municipal solid waste", "description": "This study evaluates the main effects of including 'non-ideal' bio-physical-chemical corrections in high-solids anaerobic digestion (HS-AD) of the organic fraction of municipal solid waste (OFMSW), at total solid (TS) between 10 and 40%. As a novel approach, a simple 'non-ideal' module, accounting for the effects of ionic strength (I) on the main acid-base equilibriums, was coupled to a HS-AD model, to jointly evaluate the effects of 'non-ideality' and the TS content dynamics on the HS-AD bio-physical-chemistry. 'Non-ideality' influenced the pH, concentration of inhibitors (i.e. NH3), and liquid-gas transfer (i.e. CO2), particularly at higher TS (i.e. \u2265 20%). Meanwhile, fitting the experimental data for batch assays at 15% TS showed that HS-AD of OFMSW might be operated at I\u202f\u2265\u202f0.5\u202fM. Therefore, all HS-AD simulations should account for 'non-ideal' corrections, when assessing the main inhibitory mechanisms (i.e. NH3 buildup and acidification) potentially occurring in HS-AD of OFMSW.", "keywords": ["[SDE] Environmental Sciences", "[SDV]Life Sciences [q-bio]", "high-solids anaerobic digestion model", "Solid Waste", "01 natural sciences", "7. Clean energy", "total solids dynamics", "Refuse Disposal", "12. Responsible consumption", "[SDV] Life Sciences [q-bio]", "Bioreactors", "[SDE]Environmental Sciences", "11. Sustainability", "High-solids anaerobic digestion modelNon-ideal bio-physical-chemical correctionsIonic strengthTotal solids dynamicsAmmonia inhibition", "Anaerobiosis", "ionic strength", "Methane", "ammonia inhibition", "non-ideal bio-physical-chemical corrections", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/746250/1/post-print.pdf"}, {"href": "https://hal.inrae.fr/hal-02623438/file/S0301479719303044.pdf"}, {"href": "https://doi.org/10.1016/j.jenvman.2019.03.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jenvman.2019.03.014", "name": "item", "description": "10.1016/j.jenvman.2019.03.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jenvman.2019.03.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-01T00:00:00Z"}}, {"id": "10.1186/s40793-020-00354-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:19:30Z", "type": "Journal Article", "created": "2020-03-02", "title": "Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics", "description": "Abstract                 Background                 <p>Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage two-phase biogas reactor systems at low vs. increased organic loading rates (OLRs) under mesophilic (37\uffe2\uff80\uff89\uffc2\uffb0C) and thermophilic (55\uffe2\uff80\uff89\uffc2\uffb0C) temperatures. To follow the adaptive response of the biomass-attached cellulolytic/hydrolytic biofilms at increasing ammonium/ammonia contents, genome-centered metagenomics and transcriptional profiling based on metagenome assembled genomes (MAGs) were conducted.</p>                                Results                 <p>In total, 78 bacterial and archaeal MAGs representing the most abundant members of the communities, and featuring defined quality criteria were selected and characterized in detail. Determination of MAG abundances under the tested conditions by mapping of the obtained metagenome sequence reads to the MAGs revealed that MAG abundance profiles were mainly shaped by the temperature but also by the OLR. However, the OLR effect was more pronounced for the mesophilic systems as compared to the thermophilic ones. In contrast, metatranscriptome mapping to MAGs subsequently normalized to MAG abundances showed that under thermophilic conditions, MAGs respond to increased OLRs by shifting their transcriptional activities mainly without adjusting their proliferation rates. This is a clear difference compared to the behavior of the microbiome under mesophilic conditions. Here, the response to increased OLRs involved adjusting of proliferation rates and corresponding transcriptional activities. The analysis led to the identification of MAGs positively responding to increased OLRs. The most outstanding MAGs in this regard, obviously well adapted to higher OLRs and/or associated conditions, were assigned to the order Clostridiales (Acetivibrio sp.) for the mesophilic biofilm and the orders Bacteroidales (Prevotella sp. and an unknown species), Lachnospirales (Herbinix sp. and Kineothrix sp.) and Clostridiales (Clostridium sp.) for the thermophilic biofilm. Genome-based metabolic reconstruction and transcriptional profiling revealed that positively responding MAGs mainly are involved in hydrolysis of grass silage, acidogenesis and / or\uffc2\uffa0acetogenesis.</p>                                Conclusions                 <p>An integrated -omics approach enabled the identification of new AD biofilm keystone species featuring outstanding performance under stress conditions such as increased OLRs. Genome-based knowledge on the metabolic potential and transcriptional activity of responsive microbiome members will contribute to the development of improved microbiological AD management strategies for biomethanation of renewable biomass.</p>", "keywords": ["Integrated -omics", "Bioconversion", "0301 basic medicine", "570", "Polyomics", "Integrated-omics", "Biogas", "Microbiology", "7. Clean energy", "03 medical and health sciences", "Anaerobic digestion", "GE1-350", "2. Zero hunger", "0303 health sciences", "Metagenome assembled genomes", "Microbial community structure", "15. Life on land", "QR1-502", "6. Clean water", "3. Good health", "Environmental sciences", "Metagenome assembled genomes", " Integrated -omics", " Polyomics", " Anaerobic digestion", " Biogas", " Bioconversion", " Microbial community structure", " Methane", " Metabolic activity", "13. Climate action", "Metabolic activity", "Methane", "Research Article"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1186/s40793-020-00354-x.pdf"}, {"href": "https://doi.org/10.1186/s40793-020-00354-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-020-00354-x", "name": "item", "description": "10.1186/s40793-020-00354-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-020-00354-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-02T00:00:00Z"}}, {"id": "10.1016/j.watres.2018.06.016", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:17:23Z", "type": "Journal Article", "created": "2018-06-14", "title": "High-solids anaerobic digestion model for homogenized reactors", "description": "During high-solids anaerobic digestion (HS-AD) of the organic fraction of municipal solid waste (OFMSW), an important total solid (TS) removal occurs, leading to the modification of the reactor content mass/volume, in contrast to 'wet' anaerobic digestion (AD). Therefore, HS-AD mathematical simulations need to be approached differently than 'wet' AD simulations. This study aimed to develop a modelling tool based on the anaerobic digestion model 1 (ADM1) capable of simulating the TS and the reactor mass/volume dynamics in HS-AD of OFMSW. Four hypotheses were used, including the effects of apparent concentrations at high TS. The model simulated adequately HS-AD of OFMSW in batch and continuous mode, particularly the evolution of TS, reactor mass, ammonia and volatile fatty acids. By adequately simulating the reactor content mass/volume and the TS, the HS-AD model might bring further insight about potentially inhibitory mechanisms (i.e. NH3 buildup and/or acidification) occurring in HS-AD of OFMSW.", "keywords": ["[SDE] Environmental Sciences", "ADM1", "570", "[SDV]Life Sciences [q-bio]", "High-solids anaerobic digestion", " ADM1", " Reactor mass simulation", " Total solids", " Apparent concentrations", "0211 other engineering and technologies", "High-solids anaerobic digestion", "02 engineering and technology", "Solid Waste", "01 natural sciences", "7. Clean energy", "Reactor mass simulation", "total solids", "12. Responsible consumption", "high-solids anaerobic digestion", "Bioreactors", "Ammonia", "Apparent concentrations", "reactor mass simulation", "Anaerobiosis", "0105 earth and related environmental sciences", "apparent concentrations", "Total solids", "Models", " Theoretical", "Fatty Acids", " Volatile", "6. Clean water", "Refuse Disposal", "[SDV] Life Sciences [q-bio]", "[SDE]Environmental Sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/718585/1/1-s2.0-S0043135418304603-main.pdf"}, {"href": "https://doi.org/10.1016/j.watres.2018.06.016"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2018.06.016", "name": "item", "description": "10.1016/j.watres.2018.06.016", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2018.06.016"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-10-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2019.114932", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:17:23Z", "type": "Journal Article", "created": "2019-07-30", "title": "Assessing practical identifiability during calibration and cross-validation of a structured model for high-solids anaerobic digestion", "description": "High-solids anaerobic digestion (HS-AD) of the organic fraction of municipal solid waste (OFMSW) is operated at a total solid (TS) content\u202f\u2265\u202f10% to enhance the waste treatment economy, though it might be associated to free ammonia (NH3) inhibition. This study aimed to calibrate and cross-validate a HS-AD model for homogenized reactors in order to assess the effects of high NH3 levels in HS-AD of OFMSW, but also to evaluate the suitability of the reversible non-competitive inhibition function to reproduce the effect of NH3 on the main acetogenic and methanogenic populations. The practical identifiability of structural/biochemical parameters (i.e. 35) and initial conditions (i.e. 32) was evaluated using batch experiments at different TS and/or inoculum-to-substrate ratios. Variance-based global sensitivity analysis and approximate Bayesian computation were used for parameter optimization. The experimental data in this study permitted to estimate up to 8 biochemical parameters, whereas the rest of parameters and biomass contents were poorly identifiable. The study also showed the relatively high levels of NH3 (i.e. up to 2.3\u202fg\u202fN/L) and ionic strength (i.e. up to 0.9\u202fM) when increasing TS in HS-AD of OFMSW. However, the NH3 non-competitive function was unable to capture the acetogenic/methanogenic inhibition. Therefore, the calibration emphasized the need for target-oriented experimental data to enhance the practical identifiability and the predictive capabilities of structured HS-AD models, but also the need for further testing the NH3 inhibition function used in these simulations.", "keywords": ["[SDE] Environmental Sciences", "[SDV]Life Sciences [q-bio]", "0207 environmental engineering", "high-solids anaerobic digestion model", "Bayes Theorem", "02 engineering and technology", "Solid Waste", "01 natural sciences", "7. Clean energy", "6. Clean water", "Refuse Disposal", "12. Responsible consumption", "[SDV] Life Sciences [q-bio]", "High-solids anaerobic digestion model", " ammonia inhibition", " ionic strength", " global sensitivity analysis", " approximate bayesian computation", "Bioreactors", "global sensitivity analysis", "[SDE]Environmental Sciences", "Calibration", "High-solids anaerobic digestion model", "Anaerobiosis", "ionic strength", "Methane", "ammonia inhibition", "approximate bayesian computation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/757589/1/Post-print%20for%20IRIS.pdf"}, {"href": "https://hal.inrae.fr/hal-02623443/file/S0043135419307067.pdf"}, {"href": "https://doi.org/10.1016/j.watres.2019.114932"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2019.114932", "name": "item", "description": "10.1016/j.watres.2019.114932", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2019.114932"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-01T00:00:00Z"}}, {"id": "10.1021/es3024435", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:17:31Z", "type": "Journal Article", "created": "2012-11-05", "title": "Bioenergy Production From Perennial Energy Crops: A Consequential Lca Of 12 Bioenergy Scenarios Including Land Use Changes", "description": "In the endeavor of optimizing the sustainability of bioenergy production in Denmark, this consequential life cycle assessment (LCA) evaluated the environmental impacts associated with the production of heat and electricity from one hectare of Danish arable land cultivated with three perennial crops: ryegrass (Lolium perenne), willow (Salix viminalis) and Miscanthus giganteus. For each, four conversion pathways were assessed against a fossil fuel reference: (I) anaerobic co-digestion with manure, (II) gasification, (III) combustion in small-to-medium scale biomass combined heat and power (CHP) plants and IV) co-firing in large scale coal-fired CHP plants. Soil carbon changes, direct and indirect land use changes as well as uncertainty analysis (sensitivity, MonteCarlo) were included in the LCA. Results showed that global warming was the bottleneck impact, where only two scenarios, namely willow and Miscanthus co-firing, allowed for an improvement as compared with the reference (-82 and -45 t CO\u2082-eq. ha\u207b\u00b9, respectively). The indirect land use changes impact was quantified as 310 \u00b1 170 t CO\u2082-eq. ha\u207b\u00b9, representing a paramount average of 41% of the induced greenhouse gas emissions. The uncertainty analysis confirmed the results robustness and highlighted the indirect land use changes uncertainty as the only uncertainty that can significantly change the outcome of the LCA results.", "keywords": ["Crops", " Agricultural", "Manures", "Nitrogen", "Life cycle", "Coal gasification plants", "Sus scrofa", "0211 other engineering and technologies", "Crops", "02 engineering and technology", "/dk/atira/pure/sustainabledevelopmentgoals/responsible_consumption_and_production; name=SDG 12 - Responsible Consumption and Production", "Global Warming", "7. Clean energy", "Environmental impact", "/dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energy; name=SDG 7 - Affordable and Clean Energy", "Anaerobic digestion", "11. Sustainability", "0202 electrical engineering", " electronic engineering", " information engineering", "Animals", "Anaerobiosis", "Gas emissions", "2. Zero hunger", "Fossil fuels", "Global warming", "/dk/atira/pure/sustainabledevelopmentgoals/life_on_land; name=SDG 15 - Life on Land", "Agriculture", "Carbon Dioxide", "15. Life on land", "Carbon", "Coal combustion", "Manure", "Greenhouse gases", "Carbon dioxide", "13. Climate action", "Biofuels", "Land use", "Uncertainty analysis", "Cogeneration plants", "Power generation"]}, "links": [{"href": "https://doi.org/10.1021/es3024435"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/es3024435", "name": "item", "description": "10.1021/es3024435", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/es3024435"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-11-30T00:00:00Z"}}, {"id": "21.11116/0000-0007-B312-A", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:52Z", "type": "Journal Article", "created": "2020-12-17", "title": "The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species.</p></article>", "keywords": ["Bioconversion", "anaerobic digestion", "0301 basic medicine", "2. Zero hunger", "bioconversion", "Biomethanation", "QH301-705.5", "570 Biologie", "polysaccharide utilization loci", "metabolic pathway reconstruction", "16. Peace & justice", "7. Clean energy", "Article", "660.6", "biomethanation", "03 medical and health sciences", "Metabolic pathway reconstruction", "Polysaccharide utilization loci", "Anaerobic digestion", "carbohydrate-active enzymes", "Carbohydrate-active enzymes", "ddc:570", "Biology (General)"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://doi.org/21.11116/0000-0007-B312-A"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "21.11116/0000-0007-B312-A", "name": "item", "description": "21.11116/0000-0007-B312-A", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/21.11116/0000-0007-B312-A"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-17T00:00:00Z"}}, {"id": "10.3390/microorganisms11102412", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:11Z", "type": "Journal Article", "created": "2023-09-27", "title": "Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>The current focus on renewable energy in global policy highlights the importance of methane production from biomass through anaerobic digestion (AD). To improve biomass digestion while ensuring overall process stability, microbiome-based management strategies become more important. In this study, metagenomes and metaproteomes were used for metagenomically assembled genome (MAG)-centric analyses to investigate a full-scale biogas plant consisting of three differentially operated digesters. Microbial communities were analyzed regarding their taxonomic composition, functional potential, as well as functions expressed on the proteome level. Different abundances of genes and enzymes related to the biogas process could be mostly attributed to different process parameters. Individual MAGs exhibiting different abundances in the digesters were studied in detail, and their roles in the hydrolysis, acidogenesis and acetogenesis steps of anaerobic digestion could be assigned. Methanoculleus thermohydrogenotrophicum was an active hydrogenotrophic methanogen in all three digesters, whereas Methanothermobacter wolfeii was more prevalent at higher process temperatures. Further analysis focused on MAGs, which were abundant in all digesters, indicating their potential to ensure biogas process stability. The most prevalent MAG belonged to the class Limnochordia; this MAG was ubiquitous in all three digesters and exhibited activity in numerous pathways related to different steps of AD.</p></article>", "keywords": ["anaerobic digestion", "biogas process chain", "metagenome analyses", "13. Climate action", "QH301-705.5", "metagenomic binning", "biogas microbiome", "metaproteome analyses", "15. Life on land", "Biology (General)", "7. Clean energy", "Article", "660.6"]}, "links": [{"href": "https://www.mdpi.com/2076-2607/11/10/2412/pdf"}, {"href": "https://doi.org/10.3390/microorganisms11102412"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms11102412", "name": "item", "description": "10.3390/microorganisms11102412", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms11102412"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-27T00:00:00Z"}}, {"id": "10.3390/microorganisms8122024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:12Z", "type": "Journal Article", "created": "2020-12-17", "title": "The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species.</p></article>", "keywords": ["Bioconversion", "anaerobic digestion", "0301 basic medicine", "2. Zero hunger", "bioconversion", "Biomethanation", "QH301-705.5", "570 Biologie", "polysaccharide utilization loci", "metabolic pathway reconstruction", "16. Peace & justice", "7. Clean energy", "Article", "660.6", "biomethanation", "03 medical and health sciences", "Metabolic pathway reconstruction", "Polysaccharide utilization loci", "carbohydrate-active enzymes; polysaccharide utilization loci; anaerobic digestion; biomethanation; metabolic pathway reconstruction; bioconversion", "Anaerobic digestion", "carbohydrate-active enzymes", "Carbohydrate-active enzymes", "ddc:570", "Biology (General)"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://doi.org/10.3390/microorganisms8122024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms8122024", "name": "item", "description": "10.3390/microorganisms8122024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms8122024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-17T00:00:00Z"}}, {"id": "10.3390/app11062746", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:03Z", "type": "Journal Article", "created": "2021-03-19", "title": "Halophyte Plants and Their Residues as Feedstock for Biogas Production\u2014Chances and Challenges", "description": "<p>The importance of green technologies is steadily growing. Salt-tolerant plants have been proposed as energy crops for cultivation on saline lands. Halophytes such as Salicornia europaea, Tripolium pannonicum, Crithmum maritimum and Chenopodium quinoa, among many other species, can be cultivated in saline lands, in coastal areas or for treating saline wastewater, and the biomass might be used for biogas production as an integrated process of biorefining. However, halophytes have different salt tolerance mechanisms, including compartmentalization of salt in the vacuole, leading to an increase of sodium in the plant tissues. The sodium content of halophytes may have an adverse effect on the anaerobic digestion process, which needs adjustments to achieve stable and efficient conversion of the halophytes into biogas. This review gives an overview of the specificities of halophytes that needs to be accounted for using their biomass as feedstocks for biogas plants in order to expand renewable energy production. First, the different physiological mechanisms of halophytes to grow under saline conditions are described, which lead to the characteristic composition of the halophyte biomass, which may influence the biogas production. Next, possible mechanisms to avoid negative effects on the anaerobic digestion process are described, with an overview of full-scale applications. Taking all these aspects into account, halophyte plants have a great potential for biogas and methane production with yields similar to those produced by other energy crops and the simultaneous benefit of utilization of saline soils.</p>", "keywords": ["anaerobic digestion", "0301 basic medicine", "Technology", "Inoculum adaptation", "QH301-705.5", "QC1-999", "Plant physiology", "Salicornia europaea", "Co-digestion", "7. Clean energy", "biogas production", "03 medical and health sciences", "Anaerobic digestion", "co-digestion", "Biology (General)", "Chenopodium quinoa", "QD1-999", "<i>Crithmum maritimum</i>", "2. Zero hunger", "0303 health sciences", "T", "Physics", "Sa-linity", "Crithmum maritimum", "Tripolium pannonicum", "15. Life on land", "Engineering (General). Civil engineering (General)", "Biogas production", "Dewey Decimal Classification::600 | Technik", "6. Clean water", "<i>Chenopodium quinoa</i>", "Chemistry", "13. Climate action", "TA1-2040", "Halophyte composition", "halophyte composition"]}, "links": [{"href": "https://www.mdpi.com/2076-3417/11/6/2746/pdf"}, {"href": "https://doi.org/10.3390/app11062746"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/app11062746", "name": "item", "description": "10.3390/app11062746", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/app11062746"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-18T00:00:00Z"}}, {"id": "10.3390/bioengineering6030080", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:03Z", "type": "Journal Article", "created": "2019-09-09", "title": "Comparison of Dry Versus Wet Milling to Improve Bioethanol or Methane Recovery from Solid Anaerobic Digestate", "description": "<p>Biogas plants for waste treatment valorization are presently experiencing rapid development, especially in the agricultural sector, where large amounts of digestate are being generated. In this study, we investigated the effect of vibro-ball milling (VBM) for 5 and 30 min at a frequency of 20 s\uffe2\uff88\uff921 on the physicochemical composition and enzymatic hydrolysis (30 U g\uffe2\uff88\uff921 total solids (TS) of cellulase and endo-1,4-xylanase from Trichoderma longibrachiatum) of dry and wet solid separated digestates from an agricultural biogas plant. We found that VBM of dry solid digestate improved the physical parameters as both the particle size and the crystallinity index (from 27% to 75%) were reduced. By contrast, VBM of wet solid digestate had a minimal effect on the physicochemical parameters. The best results in terms of cellulose and hemicelluloses hydrolysis were noted for 30 min of VBM of dry solid digestate, with hydrolysis yields of 64% and 85% for hemicelluloses and cellulose, respectively. At the condition of 30 min of VBM, bioethanol and methane production on the dry solid separated digestate was investigated. Bioethanol fermentation by simultaneous saccharification and fermentation resulted in an ethanol yield of 98 geth kg\uffe2\uff88\uff921 TS (corresponding to 90% of the theoretical value) versus 19 geth kg\uffe2\uff88\uff921 TS for raw solid digestate. Finally, in terms of methane potential, VBM for 30 min lead to an increase of the methane potential of 31% compared to untreated solid digestate.</p>", "keywords": ["anaerobic digestion", "Technology", "[SDV.BIO]Life Sciences [q-bio]/Biotechnology", "QH301-705.5", "sugars recovery", "Biotechnologies", "7. Clean energy", "01 natural sciences", "Article", "milling process", "12. Responsible consumption", "Autre (Chimie)", "Biology (General)", "solid digestate", "0105 earth and related environmental sciences", "660", "T", "bioethanol production ; sugars recovery ; energy balances ; solid digestate ; milling process ; anaerobic digestion", "anaerobic digestion;solid digestate;milling process;sugars recovery;energy balances;bioethanol production", "600", "540", "energy balances", "6. Clean water", "[SDV.BIO] Life Sciences [q-bio]/Biotechnology", "[CHIM.OTHE] Chemical Sciences/Other", "bioethanol production", "Other", "[CHIM.OTHE]Chemical Sciences/Other"]}, "links": [{"href": "http://www.mdpi.com/2306-5354/6/3/80/pdf"}, {"href": "https://www.mdpi.com/2306-5354/6/3/80/pdf"}, {"href": "https://doi.org/10.3390/bioengineering6030080"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioengineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/bioengineering6030080", "name": "item", "description": "10.3390/bioengineering6030080", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/bioengineering6030080"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-09-06T00:00:00Z"}}, {"id": "10.3390/fermentation8040189", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:06Z", "type": "Journal Article", "created": "2022-04-19", "title": "Halophytes as Feedstock for Biogas Production: Composition Analysis and Biomethane Potential of Salicornia spp. Plant Material from Hydroponic and Seawater Irrigation Systems", "description": "<p>The halophyte plant species Salicornia europaea and Salicornia ramosissima were investigated for their potential to serve as a substrate for biogas production. Salicornia europaea was cultivated in hydroponic systems under varying salt concentrations (0, 10, 20, and 30 g/L NaCl), while S. ramosissima was grown in greenhouse farming with aquaculture effluent irrigation. The biomethane potential of the two halophyte feedstocks was determined through batch experiments, and correlations to the plant biochemical composition were investigated. Ash and mineral content of S. europaea was correlated to the increasing salt concentration used for plant cultivation in hydroponic systems. No indication of inhibition of the anaerobic digestion process was detected for sodium concentrations of up to 2400 mg/L in the anaerobic batch-test assays. The highest biomethane yield of S. europaea of 250 mL CH4/gVS was obtained when grown under 20 g/L NaCl and up to 300 mL CH4/gVS for S. ramosissima. By concentrating the dry matter content, the biomethane yield per ton of feedstock could be increased from 24 m3 CH4/t of the fresh halophyte plant to 74 m3 CH4/t by fractionation into a pulp fraction and to 149 m3 CH4/t by drying of the plant at room temperature for 1 week.</p>", "keywords": ["anaerobic digestion", "TP500-660", "Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften", " Biologie", "biodegradability", "Fermentation industries. Beverages. Alcohol", "halophyte biomass", "biomethane potential", "7. Clean energy", "01 natural sciences", "6. Clean water", "biomethane potential; biodegradability; anaerobic digestion; halophyte biomass", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://www.mdpi.com/2311-5637/8/4/189/pdf"}, {"href": "https://www.mdpi.com/2311-5637/8/4/189/pdf"}, {"href": "https://doi.org/10.3390/fermentation8040189"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Fermentation", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/fermentation8040189", "name": "item", "description": "10.3390/fermentation8040189", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/fermentation8040189"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-18T00:00:00Z"}}, {"id": "10.3390/microorganisms9071457", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:12Z", "type": "Journal Article", "created": "2021-07-07", "title": "Indicative Marker Microbiome Structures Deduced from the Taxonomic Inventory of 67 Full-Scale Anaerobic Digesters of 49 Agricultural Biogas Plants", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>There are almost 9500 biogas plants in Germany, which are predominantly operated with energy crops and residues from livestock husbandry over the last two decades. In the future, biogas plants must be enabled to use a much broader range of input materials in a flexible and demand-oriented manner. Hence, the microbial communities will be exposed to frequently varying process conditions, while an overall stable process must be ensured. To accompany this transition, there is the need to better understand how biogas microbiomes respond to management measures and how these responses affect the process efficiency. Therefore, 67 microbiomes originating from 49 agricultural, full-scale biogas plants were taxonomically investigated by 16S rRNA gene amplicon sequencing. These microbiomes were separated into three distinct clusters and one group of outliers, which are characterized by a specific distribution of 253 indicative taxa and their relative abundances. These indicative taxa seem to be adapted to specific process conditions which result from a different biogas plant operation. Based on these results, it seems to be possible to deduce/assess the general process condition of a biogas digester based solely on the microbiome structure, in particular on the distribution of specific indicative taxa, and without knowing the corresponding operational and chemical process parameters. Perspectively, this could allow the development of detection systems and advanced process models considering the microbial diversity.</p></article>", "keywords": ["580", "anaerobic digestion", "0301 basic medicine", "2. Zero hunger", "NMDS", "0303 health sciences", "QH301-705.5", "biogas microbiome", "anaerobic digestion; biogas microbiome; taxonomic profiling; 16S rRNA gene amplicon sequencing; NMDS; indicative taxa; Pearson correlations", "Pearson correlations", "7. Clean energy", "Article", "6. Clean water", "660.6", "03 medical and health sciences", "13. Climate action", "indicative taxa", "taxonomic profiling", "Biology (General)", "16S rRNA gene amplicon sequencing"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/9/7/1457/pdf"}, {"href": "https://www.mdpi.com/2076-2607/9/7/1457/pdf"}, {"href": "https://doi.org/10.3390/microorganisms9071457"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms9071457", "name": "item", "description": "10.3390/microorganisms9071457", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms9071457"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-07-07T00:00:00Z"}}, {"id": "10.3390/su12093676", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:20Z", "type": "Journal Article", "created": "2020-05-05", "title": "Assessing New Biotechnologies by Combining TEA and TM-LCA for an Efficient Use of Biomass Resources", "description": "<p>An efficient use of biomass resources is a key element of the bioeconomy. Ideally, options leading to the highest environmental and economic gains can be singled out for any given region. In this study, to achieve this goal of singling out an ideal technology for a given region, biotechnologies are assessed by a combination of techno-economic assessment (TEA) and territorial metabolism life cycle assessment (TM-LCA). Three technology variations for anaerobic digestion (AD) were assessed at two different scales (200 kW and 1 MW) and for two different regions. First, sustainable feedstock availability for two European regions was quantified. Then, the environmental impact and economic potential of each technology when scaled up to the regional level, considering all of the region\uffe2\uff80\uff99s unique sustainably available feedstock, was investigated. Multiple criteria decision analysis and internalized damage monetization were used to generate single scores for the assessments. Preference for the technology scenario producing the most energy was shown for all regions and scales, while producing bioplastic was less preferable since the value of the produced bioplastic plastic was not great enough to offset the resultant reduction in energy production. Assessing alternatives in a regional context provided valuable information about the influence of different types of feedstock on environmental performance.</p>", "keywords": ["Techno-economic assessment", "anaerobic digestion", "Regional assessment", "0211 other engineering and technologies", "Biogas", "techno-economic assessment", "02 engineering and technology", "/dk/atira/pure/sustainabledevelopmentgoals/responsible_consumption_and_production; name=SDG 12 - Responsible Consumption and Production", "biomass valorization", "7. Clean energy", "territorial metabolism", "12. Responsible consumption", "regional assessment", "Life cycle assessment", "/dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energy; name=SDG 7 - Affordable and Clean Energy", "life cycle assessment", "Anaerobic digestion", "11. Sustainability", "biogas", "0202 electrical engineering", " electronic engineering", " information engineering", "Territorial metabolism", "Polyhydroxyalkanoates", "polyhydroxyalkanoates", "Wet oxidation", "Biomass valorization", "wet oxidation", "13. Climate action", "8. Economic growth"]}, "links": [{"href": "http://www.mdpi.com/2071-1050/12/9/3676/pdf"}, {"href": "https://www.mdpi.com/2071-1050/12/9/3676/pdf"}, {"href": "https://doi.org/10.3390/su12093676"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/su12093676", "name": "item", "description": "10.3390/su12093676", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/su12093676"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-02T00:00:00Z"}}, {"id": "10.3390/su152416948", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:21Z", "type": "Journal Article", "created": "2023-12-18", "title": "Microalgae Production on Biogas Digestate in Sub-Alpine Region of Europe\u2014Development of Simple Management Decision Support Tool", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>In a one-and-a-half-year study conducted in the ALS6 region in Europe (Ljubljana, Slovenia), the cultivation of microalgae in anaerobic digestate from food waste, mainly Scenedesmus dimorphus and Scenedesmus quadricauda, was investigated in three ponds (1260 L each) under a greenhouse. The effects of changing digestate quality and quantity as well as seasonal fluctuations on the productivity of the microalgae were investigated in three stages: Learning/Design (SI), Testing (SII), and Verification/Calibration (SIII). A decision support tool (DST) was developed using easy-to-measure parameters such as pH, temperature, electrical conductivity, mineral nitrogen forms and physical, biological parameters (OD, delayed fluorescence intensity). To control optimal pond operation, we proposed the photosynthetic culture index (PCI) as an early indicator for necessary interventions. Flocculation and nitrite levels (above 3 mg NO2-N L\u22121) were signals for the immediate remediation of the algae culture. Under optimal conditions in summer SIII, an average algal biomass production of 11 \u00b1 1.5 g m\u22122 day\u22121 and a nitrogen use efficiency of 28 \u00b1 2.6 g biomass/g N-input were achieved with the developed DST. The developed DST tool was, in this study, successfully implemented and used for the cultivation of microalgae consortia predominated by Scenedesmus dimorphus and S. quadricauda with biogas digestate. DST offers the possibility to be modified according to producers\u2019 specific needs, facility, digestate and climate conditions, and as such, could be used for different microalgae cultivation processes with biogas digestate as a food source.</p></article>", "keywords": ["anaerobic digestion", "2. Zero hunger", "0106 biological sciences", "decision support tool", "anaerobna presnova", "circular economy", "orodja za podporo odlo\u010danju", "15. Life on land", "7. Clean energy", "01 natural sciences", "microalgae Scenedesmus", "6. Clean water", "open ponds", "circular economy", " anaerobic digestion", " food waste digestate", " microalgae Scenedesmus", " open ponds", " decision support tool", "kro\u017eno gospodarstvo", "digestat \u017eivilskih odpadkov", "mikroalge", "13. Climate action", "\u017eivilski odpadki", "food waste digestate", "kro\u017eno gospodarstvo", " anaerobna presnova", " digestat \u017eivilskih odpadkov", " \u017eivilski odpadki", " mikroalge", " Scenedesmus", " odprti ribniki", " orodja za podporo odlo\u010danju", "info:eu-repo/classification/udc/63", "odprti ribniki", "Scenedesmus", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.mdpi.com/2071-1050/15/24/16948/pdf"}, {"href": "https://doi.org/10.3390/su152416948"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/su152416948", "name": "item", "description": "10.3390/su152416948", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/su152416948"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-18T00:00:00Z"}}, {"id": "10.5281/zenodo.3371224", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:23:28Z", "type": "Report", "title": "Circular Agronomics: producing high-quality organic fertilizers from digestates in an agro-industrial biogas plant", "description": "Six high-value organic fertilizers (OF) will be produce in a combined process based on anaerobic digestion of pig manure with agro-industrial organic wastes, followed by an efficient solid-liquid separation (centrifuge) and solar drying. The digestate centrates will be submitted to a stripping treatment to produce two liquid streams, one of them enriched in N and available for fertilization. The mass (C, N, P, K) and energy balances will be performed, including gaseous emission monitoring, along the production of different OFs. Among physic-chemical parameters, the agronomical potential of such products will be tested in field crop rotations, as well as horticultural crops. The overall process of OFs production will be characterized, based on compiled data from balances, emissions and products\u00b4 yield and characteristics. Finally, once the OFs production process will be well defined, these data will feed a Life Cycle Analysis assessment. This digestate valorization process is one of the case studies of the European project \u2018Circular Agronomics\u2019.", "keywords": ["2. Zero hunger", "Organic fertilizers", " anaerobic digestion", " solar drying.", "13. Climate action", "7. Clean energy", "12. Responsible consumption"], "contacts": [{"organization": "Arenas, Victor Riau, Gual, Lluis Morey, Porta, \u00c1ngel, Soler, Joan, Fern\u00e1ndez, Bel\u00e9n,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.3371224"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.3371224", "name": "item", "description": "10.5281/zenodo.3371224", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.3371224"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-19T00:00:00Z"}}, {"id": "10033/622632", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:24:41Z", "type": "Journal Article", "created": "2020-03-02", "title": "Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics", "description": "Abstract                 Background                 <p>Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage two-phase biogas reactor systems at low vs. increased organic loading rates (OLRs) under mesophilic (37\uffe2\uff80\uff89\uffc2\uffb0C) and thermophilic (55\uffe2\uff80\uff89\uffc2\uffb0C) temperatures. To follow the adaptive response of the biomass-attached cellulolytic/hydrolytic biofilms at increasing ammonium/ammonia contents, genome-centered metagenomics and transcriptional profiling based on metagenome assembled genomes (MAGs) were conducted.</p>                                Results                 <p>In total, 78 bacterial and archaeal MAGs representing the most abundant members of the communities, and featuring defined quality criteria were selected and characterized in detail. Determination of MAG abundances under the tested conditions by mapping of the obtained metagenome sequence reads to the MAGs revealed that MAG abundance profiles were mainly shaped by the temperature but also by the OLR. However, the OLR effect was more pronounced for the mesophilic systems as compared to the thermophilic ones. In contrast, metatranscriptome mapping to MAGs subsequently normalized to MAG abundances showed that under thermophilic conditions, MAGs respond to increased OLRs by shifting their transcriptional activities mainly without adjusting their proliferation rates. This is a clear difference compared to the behavior of the microbiome under mesophilic conditions. Here, the response to increased OLRs involved adjusting of proliferation rates and corresponding transcriptional activities. The analysis led to the identification of MAGs positively responding to increased OLRs. The most outstanding MAGs in this regard, obviously well adapted to higher OLRs and/or associated conditions, were assigned to the order Clostridiales (Acetivibrio sp.) for the mesophilic biofilm and the orders Bacteroidales (Prevotella sp. and an unknown species), Lachnospirales (Herbinix sp. and Kineothrix sp.) and Clostridiales (Clostridium sp.) for the thermophilic biofilm. Genome-based metabolic reconstruction and transcriptional profiling revealed that positively responding MAGs mainly are involved in hydrolysis of grass silage, acidogenesis and / or\uffc2\uffa0acetogenesis.</p>                                Conclusions                 <p>An integrated -omics approach enabled the identification of new AD biofilm keystone species featuring outstanding performance under stress conditions such as increased OLRs. Genome-based knowledge on the metabolic potential and transcriptional activity of responsive microbiome members will contribute to the development of improved microbiological AD management strategies for biomethanation of renewable biomass.</p>", "keywords": ["Integrated -omics", "Bioconversion", "0301 basic medicine", "2. Zero hunger", "570", "0303 health sciences", "Polyomics", "Integrated-omics", "Metagenome assembled genomes", "Biogas", "Microbial community structure", "15. Life on land", "Microbiology", "7. Clean energy", "QR1-502", "6. Clean water", "3. Good health", "Environmental sciences", "03 medical and health sciences", "13. Climate action", "Anaerobic digestion", "Metabolic activity", "GE1-350", "Methane", "Research Article"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1186/s40793-020-00354-x.pdf"}, {"href": "https://doi.org/10033/622632"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10033/622632", "name": "item", "description": "10033/622632", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10033/622632"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-02T00:00:00Z"}}, {"id": "20.500.12556/RUL-153431", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:47Z", "type": "Journal Article", "created": "2023-12-18", "title": "Microalgae Production on Biogas Digestate in Sub-Alpine Region of Europe\u2014Development of Simple Management Decision Support Tool", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>In a one-and-a-half-year study conducted in the ALS6 region in Europe (Ljubljana, Slovenia), the cultivation of microalgae in anaerobic digestate from food waste, mainly Scenedesmus dimorphus and Scenedesmus quadricauda, was investigated in three ponds (1260 L each) under a greenhouse. The effects of changing digestate quality and quantity as well as seasonal fluctuations on the productivity of the microalgae were investigated in three stages: Learning/Design (SI), Testing (SII), and Verification/Calibration (SIII). A decision support tool (DST) was developed using easy-to-measure parameters such as pH, temperature, electrical conductivity, mineral nitrogen forms and physical, biological parameters (OD, delayed fluorescence intensity). To control optimal pond operation, we proposed the photosynthetic culture index (PCI) as an early indicator for necessary interventions. Flocculation and nitrite levels (above 3 mg NO2-N L\u22121) were signals for the immediate remediation of the algae culture. Under optimal conditions in summer SIII, an average algal biomass production of 11 \u00b1 1.5 g m\u22122 day\u22121 and a nitrogen use efficiency of 28 \u00b1 2.6 g biomass/g N-input were achieved with the developed DST. The developed DST tool was, in this study, successfully implemented and used for the cultivation of microalgae consortia predominated by Scenedesmus dimorphus and S. quadricauda with biogas digestate. DST offers the possibility to be modified according to producers\u2019 specific needs, facility, digestate and climate conditions, and as such, could be used for different microalgae cultivation processes with biogas digestate as a food source.</p></article>", "keywords": ["anaerobic digestion", "2. Zero hunger", "0106 biological sciences", "decision support tool", "anaerobna presnova", "circular economy", "orodja za podporo odlo\u010danju", "15. Life on land", "7. Clean energy", "01 natural sciences", "microalgae Scenedesmus", "6. Clean water", "open ponds", "kro\u017eno gospodarstvo", "digestat \u017eivilskih odpadkov", "mikroalge", "13. Climate action", "\u017eivilski odpadki", "food waste digestate", "info:eu-repo/classification/udc/63", "odprti ribniki", "Scenedesmus", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.mdpi.com/2071-1050/15/24/16948/pdf"}, {"href": "https://doi.org/20.500.12556/RUL-153431"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.12556/RUL-153431", "name": "item", "description": "20.500.12556/RUL-153431", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.12556/RUL-153431"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-18T00:00:00Z"}}, {"id": "21.11116/0000-0008-DFA2-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:52Z", "type": "Journal Article", "created": "2021-07-07", "title": "Indicative Marker Microbiome Structures Deduced from the Taxonomic Inventory of 67 Full-Scale Anaerobic Digesters of 49 Agricultural Biogas Plants", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>There are almost 9500 biogas plants in Germany, which are predominantly operated with energy crops and residues from livestock husbandry over the last two decades. In the future, biogas plants must be enabled to use a much broader range of input materials in a flexible and demand-oriented manner. Hence, the microbial communities will be exposed to frequently varying process conditions, while an overall stable process must be ensured. To accompany this transition, there is the need to better understand how biogas microbiomes respond to management measures and how these responses affect the process efficiency. Therefore, 67 microbiomes originating from 49 agricultural, full-scale biogas plants were taxonomically investigated by 16S rRNA gene amplicon sequencing. These microbiomes were separated into three distinct clusters and one group of outliers, which are characterized by a specific distribution of 253 indicative taxa and their relative abundances. These indicative taxa seem to be adapted to specific process conditions which result from a different biogas plant operation. Based on these results, it seems to be possible to deduce/assess the general process condition of a biogas digester based solely on the microbiome structure, in particular on the distribution of specific indicative taxa, and without knowing the corresponding operational and chemical process parameters. Perspectively, this could allow the development of detection systems and advanced process models considering the microbial diversity.</p></article>", "keywords": ["580", "anaerobic digestion", "2. Zero hunger", "0301 basic medicine", "NMDS", "0303 health sciences", "QH301-705.5", "biogas microbiome", "Pearson correlations", "7. Clean energy", "Article", "6. Clean water", "660.6", "03 medical and health sciences", "13. Climate action", "indicative taxa", "taxonomic profiling", "Biology (General)", "16S rRNA gene amplicon sequencing"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/9/7/1457/pdf"}, {"href": "https://www.mdpi.com/2076-2607/9/7/1457/pdf"}, {"href": "https://doi.org/21.11116/0000-0008-DFA2-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "21.11116/0000-0008-DFA2-6", "name": "item", "description": "21.11116/0000-0008-DFA2-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/21.11116/0000-0008-DFA2-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-07-07T00:00:00Z"}}, {"id": "21.11116/0000-000D-E197-A", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:53Z", "type": "Journal Article", "created": "2023-09-27", "title": "Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>The current focus on renewable energy in global policy highlights the importance of methane production from biomass through anaerobic digestion (AD). To improve biomass digestion while ensuring overall process stability, microbiome-based management strategies become more important. In this study, metagenomes and metaproteomes were used for metagenomically assembled genome (MAG)-centric analyses to investigate a full-scale biogas plant consisting of three differentially operated digesters. Microbial communities were analyzed regarding their taxonomic composition, functional potential, as well as functions expressed on the proteome level. Different abundances of genes and enzymes related to the biogas process could be mostly attributed to different process parameters. Individual MAGs exhibiting different abundances in the digesters were studied in detail, and their roles in the hydrolysis, acidogenesis and acetogenesis steps of anaerobic digestion could be assigned. Methanoculleus thermohydrogenotrophicum was an active hydrogenotrophic methanogen in all three digesters, whereas Methanothermobacter wolfeii was more prevalent at higher process temperatures. Further analysis focused on MAGs, which were abundant in all digesters, indicating their potential to ensure biogas process stability. The most prevalent MAG belonged to the class Limnochordia; this MAG was ubiquitous in all three digesters and exhibited activity in numerous pathways related to different steps of AD.</p></article>", "keywords": ["anaerobic digestion", "biogas process chain", "metagenome analyses", "13. Climate action", "QH301-705.5", "metagenomic binning", "biogas microbiome", "metaproteome analyses", "15. Life on land", "Biology (General)", "7. Clean energy", "Article", "660.6"]}, "links": [{"href": "https://www.mdpi.com/2076-2607/11/10/2412/pdf"}, {"href": "https://doi.org/21.11116/0000-000D-E197-A"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "21.11116/0000-000D-E197-A", "name": "item", "description": "21.11116/0000-000D-E197-A", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/21.11116/0000-000D-E197-A"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-27T00:00:00Z"}}, {"id": "38554957", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:26:50Z", "type": "Journal Article", "created": "2024-03-29", "title": "Effect of manure co-digestion on methane production, carbon retention, and fertilizer value of digestate", "description": "Anaerobic digestion can provide benefits not only from the perspective of renewable energy production but also in the form of fertilization effect and increased retention of C in soils after digestate application. This study consisted of two phases, where the first phase assessed the suitability of carbon-rich co-feedstocks for methane production via laboratory testing. The second phase assessed the balance and stability of C before and after anaerobic digestion by systematic digestate characterization, and by evaluating its carbon retention potential using a modeling approach. The results indicated that pyrolysis chars had a negligible effect on the methane production potential of cattle manure, while wheat straw expectedly increased methane production. Thus, a mixture of cattle manure and wheat straw was digested in pilot-scale leach-bed reactors and compared with undigested manure and straw. Although the total amount of C in the digestate was lower than in the untreated feedstocks, the digestion process stabilized C and was modeled to be more effective in retaining C in the soil than untreated cattle manure and wheat straw. In addition, digestion converted 23-27\u00a0% of the C into valuable methane, increasing the valorization of the total C in the feedstock. Considering anaerobic digestion processes as a strategy to optimize both carbon and nutrient valorization provides a more holistic approach to addressing climate change and improving soil health.", "keywords": ["anaerobic digestion", "straw", "630", "333", "Carbon", "Manure", "Soil", "nutrients", "biogas", "Animals", "Cattle", "Anaerobiosis", "soil carbon", "Fertilizers", "Methane", "Triticum"]}, "links": [{"href": "https://doi.org/38554957"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "38554957", "name": "item", "description": "38554957", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/38554957"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-06-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=anaerobic+digestion&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=anaerobic+digestion&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=anaerobic+digestion&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=anaerobic+digestion&offset=25", "hreflang": "en-US"}], "numberMatched": 25, "numberReturned": 25, "distributedFeatures": [], "timeStamp": "2026-05-30T15:15:37.718875Z"}