Selectively fluorinated compounds are found frequently in pharmaceutical and agrochemical products where currently 25\uffe2\uff80\uff9330\uffe2\uff80\uff89% of optimised compounds emerge from development containing at least one fluorine atom. There are many methods for the site\uffe2\uff80\uff90specific introduction of fluorine, but all are chemical and they often use environmentally challenging reagents. Biochemical processes for C\uffe2\uff88\uff92F bond formation are attractive, but they are extremely rare. In this work, the fluorinase enzyme, originally identified from the actinomycete bacterium Streptomyces cattleya, is engineered into Escherichia coli in such a manner that the organism is able to produce 5\uffe2\uff80\uffb2\uffe2\uff80\uff90fluorodeoxyadenosine (5\uffe2\uff80\uffb2\uffe2\uff80\uff90FDA) from S\uffe2\uff80\uff90adenosyl\uffe2\uff80\uff90l\uffe2\uff80\uff90methionine (SAM) and fluoride in live E.\uffe2\uff80\uff85coli cells. Success required the introduction of a SAM transporter and deletion of the endogenous fluoride efflux capacity in order to generate an E.\uffe2\uff80\uff85coli host that has the potential for future engineering of more elaborate fluorometabolites.
", "keywords": ["SAM transporters", "0301 basic medicine", "570", "S-Adenosylmethionine", "0303 health sciences", "Deoxyadenosines", "Halogenation", "DAS", "Fluorine", "Halogenations", "540", "QD Chemistry", "Streptomyces", "3. Good health", "03 medical and health sciences", "Bacterial Proteins", "Isomerism", "Escherichia coli", "QD", "Fluoride channels", "Genetic Engineering", "Oxidoreductases", "Fluorinases"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.202000051"}, {"href": "https://doi.org/10.1002/cbic.202000051"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ChemBioChem", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/cbic.202000051", "name": "item", "description": "10.1002/cbic.202000051", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/cbic.202000051"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-03T00:00:00Z"}}, {"id": "10.1016/j.colsurfb.2023.113433", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:16:22Z", "type": "Journal Article", "created": "2023-06-28", "title": "Contrasting transport and fate of hydrophilic and hydrophobic bacteria in wettable and water-repellent porous media: Straining or attachment?", "description": "Bacterial transport and retention likely depend on bacterial and soil surface properties, especially hydrophobicity. We used a controlled experimental setup to explore hydrophilic Escherichia coli (E. coli) and hydrophobic Rhodococcus erythropolis (PTCC1767) (R. erythropolis) transport through dry (-\u00a015,000\u00a0cm water potential) and water saturated (0\u00a0cm water potential) wettable and water-repellent sand columns. A pulse of bacteria (1\u00a0\u00d7\u00a0108 CFU mL-1) and bromide (10\u00a0mmol\u00a0L-1) moved through the columns under saturated flow (0\u00a0cm) for four pore volumes. A second bacteria and bromide pulse was then poured on the column surfaces and leaching was extended six more pore volumes. In dry wettable sand attachment dominated E. coli retention, whereas R. erythropolis was dominated by straining. Once wetted, the dominant retention mechanisms flipped between these bacteria. Attachment by either bacteria decreased markedly in water-repellent sand, so straining was the main retention mechanism. We explain this from capillary potential energy, which enhanced straining under the formation of water films at very early times (i.e., imbibing) and film thinning at much later times (i.e., draining). The interaction between the hydrophobicity of bacteria and soil on transport, retention and release mechanisms needs greater consideration in predictions.", "keywords": ["Bromides", "2040 Environment and Biodiversity", "570", "Supplementary Information", "Wetting characteristics", "Vadose zone", "610", "Soil", "Colloid and Surface Chemistry", "Sand", "Pore-scale processes", "Escherichia coli", "Physical and Theoretical Chemistry", "European Commission", "101026287", "SDG 15 - Life on Land", "Drought", "T", "Water", "Surfaces and Interfaces", "T Technology", "Interfacial processes", "3. Good health", "TC Hydraulic engineering. Ocean engineering", "Marie Sklodowska-Curie grant", "EU Horizon 2020", "SDG 6 - Clean Water and Sanitation", "TC", "Porosity", "Hydrophobic and Hydrophilic Interactions", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1016/j.colsurfb.2023.113433"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Colloids%20and%20Surfaces%20B%3A%20Biointerfaces", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.colsurfb.2023.113433", "name": "item", "description": "10.1016/j.colsurfb.2023.113433", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.colsurfb.2023.113433"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-08-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2018.09.128", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:16:36Z", "type": "Journal Article", "created": "2018-09-28", "title": "A rationale for the high limits of quantification of antibiotic resistance genes in soil", "description": "The determination of values of abundance of antibiotic resistance genes (ARGs) per mass of soil is extremely useful to assess the potential impacts of relevant sources of antibiotic resistance, such as irrigation with treated wastewater or manure application. Culture-independent methods and, in particular, quantitative PCR (qPCR), have been regarded as suitable approaches for such a purpose. However, it is arguable if these methods are sensitive enough to measure ARGs abundance at levels that may represent a risk for environmental and human health. This study aimed at demonstrating the range of values of ARGs quantification that can be expected based on currently used procedures of DNA extraction and qPCR analyses. The demonstration was based on the use of soil samples spiked with known amounts of wastewater antibiotic resistant bacteria (ARB) (Enterococcus faecalis, Escherichia coli, Acinetobacter johnsonii, or Pseudomonas aeruginosa), harbouring known ARGs, and also on the calculation of expected values determined based on qPCR. The limits of quantification (LOQ) of the ARGs (vanA, qnrS, blaTEM, blaOXA, blaIMP, blaVIM) were observed to be approximately 4 log-units per gram of soil dry weight, irrespective of the type of soil tested. These values were close to the theoretical LOQ values calculated based on currently used DNA extraction methods and qPCR procedures. The observed LOQ values can be considered extremely high to perform an accurate assessment of the impacts of ARGs discharges in soils. A key message is that ARGs accumulation will be noticeable only at very high doses. The assessment of the impacts of ARGs discharges in soils, of associated risks of propagation and potential transmission to humans, must take into consideration this type of evidence, and avoid the simplistic assumption that no detection corresponds to risk absence.", "keywords": ["0301 basic medicine", "2. Zero hunger", "LOD - Limit of detection", "0303 health sciences", "Acinetobacter", "Drug Resistance", " Microbial", "Wastewater", "Real-Time Polymerase Chain Reaction", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Manure", "Quantitative PCR", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "13. Climate action", "Pseudomonas aeruginosa", "Enterococcus faecalis", "Escherichia coli", "LOQ - Limit of quantification", "Soil Microbiology", "Risk assessment"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2018.09.128"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2018.09.128", "name": "item", "description": "10.1016/j.envpol.2018.09.128", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2018.09.128"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-01T00:00:00Z"}}, {"id": "10.1016/j.fcr.2012.09.011", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-05-30T16:16:39Z", "type": "Journal Article", "created": "2012-10-23", "title": "Upland Rice Production Under Conservation Agriculture Cropping Systems In Cold Conditions Of Tropical Highlands", "description": "In response to the extensive development of upland rice on the hillsides of the Malagasy highlands, alternative cropping systems based on conservation agriculture have been recommended to halt loss of soil fertility. To assess the yield performances of these cropping systems, an experiment was set up in 2003 at Andranomanelatra (1640 m asl) in the Malagasy highlands. Grain yield, yield components, biomass accumulation and nitrogen uptake of upland rice were analyzed in the 2004-2005, 2006-2006, and 2006-2007 seasons, and root length density was measured in the 2007-2008 season. The rice crop was planted every second year following two different crops: maize intercropped with soybean (M + S, with both conventional tillage and no tillage) and maize intercropped with Brachiaria ruziziensis (M + B only with no tillage). For each cropping system, two levels of fertilization were used: no fertilizer or application of organic inputs and mineral fertilizer. The season, cropping system, and fertilization treatment had significant effects on rice grain yields. Higher yields were associated with a greater number of plants per m2, which decreased significantly over the three seasons, probably due to the highly variable beginning of the rains, and in the final season, with attacks by soil insects. The rice yield with conventional tillage was the highest and differed significantly from rice yield when maize was intercropped with Brachiaria under the no-till system, but not when the maize was intercropped with soybean with no tillage. In all three seasons, grain yields were closely linked to crop N at harvest. Differences in N uptake between treatments appeared very early in the crop cycle. Under conventional tillage, root length density at 68 days after sowing was higher between 0 and 30 cm depth than with no tillage. In these cold highlands conditions, plant establishment appeared to be more difficult with no tillage and resulted in reduced plant development and plant N uptake, particularly when rice was planted after maize intercropped with Brachiaria.", "keywords": ["[SDE] Environmental Sciences", "0106 biological sciences", "Glycine max", "F08 - Syst\u00e8mes et modes de culture", "rendement des cultures", "F50 - Anatomie et morphologie des plantes", "Zea mays", "01 natural sciences", "630", "fertilisation", "http://aims.fao.org/aos/agrovoc/c_10176", "http://aims.fao.org/aos/agrovoc/c_16034", "http://aims.fao.org/aos/agrovoc/c_8076", "syst\u00e8me de culture", "http://aims.fao.org/aos/agrovoc/c_3301", "http://aims.fao.org/aos/agrovoc/c_4510", "http://aims.fao.org/aos/agrovoc/c_8511", "http://aims.fao.org/aos/agrovoc/c_10795", "F07 - Fa\u00e7ons culturales", "2. Zero hunger", "syst\u00e8me racinaire", "engrais azot\u00e9", "http://aims.fao.org/aos/agrovoc/c_26419", "Oryza", "non-travail du sol", "04 agricultural and veterinary sciences", "15. Life on land", "riz pluvial", "rotation culturale", "6. Clean water", "Brachiaria ruziziensis", "densit\u00e9", "http://aims.fao.org/aos/agrovoc/c_2186", "http://aims.fao.org/aos/agrovoc/c_8504", "http://aims.fao.org/aos/agrovoc/c_5435", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries", "http://aims.fao.org/aos/agrovoc/c_1971", "http://aims.fao.org/aos/agrovoc/c_5195", "http://aims.fao.org/aos/agrovoc/c_6662", "F04 - Fertilisation"]}, "links": [{"href": "https://doi.org/10.1016/j.fcr.2012.09.011"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Field%20Crops%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.fcr.2012.09.011", "name": "item", "description": "10.1016/j.fcr.2012.09.011", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.fcr.2012.09.011"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-10-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.176196", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:17:23Z", "type": "Journal Article", "created": "2024-09-13", "title": "Modeling bacterial transport and fate: Insight into the cascading consequences of soil water repellency and contrasting hydraulic conditions", "description": "The mechanisms governing bacteria transport and fate rely on their hydrophobicity and the wettability of porous media across a wide range of soil moisture conditions, extending from extreme dryness to highly saturated states. However, it largely remains unknown how transport, retention, and release mechanisms change in natural soil systems in such conditions. We thus optimized our previously published unique transport data for hydrophilic Escherichia coli (E. coli) and hydrophobic Rhodococcus erythropolis (R. erythropolis) bacteria, and bromide (Br-) in two distinct wettable and water-repellent soils at column scale. The soils were initially dry, followed by injecting influents in two pulses followed by a flushing step under saturated flow conditions for six pore volumes. We conducted simulations for each pulse separately and simultaneously for soils. There were differences in hydraulic properties of the soils due to their contrasting wetting characteristic in separate and simultaneously modeling of each pulse affecting Br- and bacteria transport fate. Bacteria attachment was the dominant retention mechanism in both soils in these conditions. Notably, the 82.4\u00a0min-1 attachment rate in wettable soil was almost 10\u00d7 greater than in the water-repellent soil and it governed optimization of bacteria die-off. Physicochemical detachment and physical release unraveled the effect of bacteria size and hydrophobicity interacting with soil wettability. The smaller and hydrophobic R. erythropolis detached more easily while hydrophilic E. coli released; the rates were enhanced by soil water repellency. Further research is needed to reveal the effects of surface wettability properties on bacteria survival especially at the nanoscale.", "keywords": ["690", "Bromides", "Bacteria", "QH301 Biology", "Transport processes", "610", "Attachment", "Water", "QH301", "Water repellency", "Soil", "Straining", "Escherichia coli", "Wettability", "Rhodococcus", "Hydrophobic and Hydrophilic Interactions", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2024.176196"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.176196", "name": "item", "description": "10.1016/j.scitotenv.2024.176196", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.176196"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.1016/j.still.2010.07.011", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:17:44Z", "type": "Journal Article", "created": "2010-08-15", "title": "Impact Of Pasture, Agriculture And Crop-Livestock Systems On Soil C Stocks In Brazil", "description": "Abstract Changes in land use can result in either sources or sinks of atmospheric carbon (C), depending on management practices. In Brazil, significant changes in land use result from the conversion of native vegetation to pasture and agriculture, conversion of pasture to agriculture and, more recently, the conversion of pasture and agriculture to integrated crop-livestock systems (ICL). The ICL system proposes a diversity of activities that include the strategic incorporation of pastures to agriculture so as to benefit both. In agricultural areas, for example, the implementation of ICL requires the production of quality forage for animals between crops as well as the production of straw to facilitate the sustainability of the no-tillage (NT) management system. The objective of this study was to evaluate the modifications in soil C stocks resulting from the main processes involved in the changes of land use in Amazonia and Cerrado biomes. For comparison purposes, areas under native vegetation, pastures, crop succession and ICL under different edapho-climatic conditions in Amazonia and Cerrado biomes were evaluated. This study demonstrated that the conversion of native vegetation to pasture can cause the soil to function either as a source or a sink of atmospheric CO2, depending on the land management applied. Non-degraded pasture under fertile soil showed a mean accumulation rate of 0.46\u00a0g\u00a0ha\u22121\u00a0year\u22121. Carbon losses from pastures implemented in naturally low fertile soil ranged from 0.15 to 1.53\u00a0Mg\u00a0ha\u22121\u00a0year\u22121, respectively, for non-degraded and degraded pasture. The conversion of native vegetation to agriculture in areas under the ICL system, even when cultivated under NT, resulted in C losses of 1.31 in six years and of 0.69\u00a0Mg\u00a0ha\u22121 in 21 years. The conversion of a non-degraded pasture to cropland (soybean/sorghum) released, in average, 1.44 Mg of C ha\u22121year\u22121to the atmosphere. The ICL system in agricultural areas has shown evidences that it always functions as a sink of C with accumulation rates ranging from 0.82 to 2.58\u00a0Mg\u00a0ha\u22121\u00a0year\u22121. The ICL produces soil C accumulation and, as a consequence, reduces atmospheric CO2 in areas formerly cultivated under crop succession. However, the magnitude of C accumulation in soil depends on factors such as the types of crops, the edapho-climatic conditions and the amount of time the area is under ICL.", "keywords": ["[SDV.SA]Life Sciences [q-bio]/Agricultural sciences", "550", "limiting water range", "01 natural sciences", "630", "atlantic forest", "Amazonia", "Crop-livestock systems", "Land use change", "0105 earth and related environmental sciences", "2. Zero hunger", "[SDV.SA] Life Sciences [q-bio]/Agricultural sciences", "region", "Crop-livestock", "native cerrado", "organic-carbon sequestration", "grassland management", "nitrogen stocks", "Cerrado", "04 agricultural and veterinary sciences", "15. Life on land", "greenhouse-gas emissions", "matter", "6. Clean water", "brachiaria pastures", "Soil carbon stock", "13. Climate action", "tillage", "systems", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1016/j.still.2010.07.011"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20and%20Tillage%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.still.2010.07.011", "name": "item", "description": "10.1016/j.still.2010.07.011", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.still.2010.07.011"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-09-01T00:00:00Z"}}, {"id": "10.1016/j.still.2013.02.008", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-05-30T16:17:46Z", "type": "Journal Article", "created": "2013-03-19", "title": "Cover Crops And No-Till Effects On Physical Fractions Of Soil Organic Matter", "description": "Brazilian Agricultural Research Corporation (EMBRAPA) Rice and Beans Research Center, Santo Antonio de Goias, GO", "keywords": ["land use change", "Soil management", "Aggregates", "Millet", "fallow", "grass", "Cultivation", "Soil pollution", "soil depth", "Crops", "cover crop", "Plants (botany)", "soil organic matter", "Organic compounds", "soil quality", "zero tillage", "Agricultural machinery", "soil aggregate", "Panicum maximum", "2. Zero hunger", "soil surface", "rice", "Brachiaria brizantha", "Biological materials", "04 agricultural and veterinary sciences", "Biogeochemistry", "15. Life on land", "sustainability", "Agronomy", "Brachiaria ruziziensis", "13. Climate action", "Soils", "conservation tillage", "0401 agriculture", " forestry", " and fisheries", "total organic carbon", "plowing"]}, "links": [{"href": "https://doi.org/10.1016/j.still.2013.02.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20and%20Tillage%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.still.2013.02.008", "name": "item", "description": "10.1016/j.still.2013.02.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.still.2013.02.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-06-01T00:00:00Z"}}, {"id": "10.1016/j.ultsonch.2022.105919", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:17:52Z", "type": "Journal Article", "created": "2022-01-18", "title": "Bacterial cell wall material properties determine E. coli resistance to sonolysis", "description": "The applications of bacterial sonolysis in industrial settings are plagued by the lack of the knowledge of the exact mechanism of action of sonication on bacterial cells, variable effectiveness of cavitation on bacteria, and inconsistent data of its efficiency. In this study we have systematically changed material properties of E. coli cells to probe the effect of different cell wall layers on bacterial resistance to ultrasonic irradiation (20\u00a0kHz, output power 6,73\u00a0W, horn type, 3\u00a0mm probe tip diameter, 1\u00a0ml sample volume). We have determined the rates of sonolysis decay for bacteria with compromised major capsular polymers, disrupted outer membrane, compromised peptidoglycan layer, spheroplasts, giant spheroplasts, and in bacteria with different cell physiology. The non-growing bacteria were 5-fold more resistant to sonolysis than growing bacteria. The most important bacterial cell wall structure that determined the outcome during sonication was peptidoglycan. If peptidoglycan was remodelled, weakened, or absent the cavitation was very efficient. Cells with removed peptidoglycan had sonolysis resistance equal to lipid vesicles and were extremely sensitive to sonolysis. The results suggest that bacterial physiological state as well as cell wall architecture are major determinants that influence the outcome of bacterial sonolysis.", "keywords": ["sonication", "0301 basic medicine", "cell envelope", "Cell envelope", "ultrazvo\u010dna sonikacija", "Short Communication", "celi\u010dna ovojnica", "QC221-246", "Peptidoglycan", "viability", " bacteria", " Escherichia coli", " sonication", " cell envelope", "Sonication", "03 medical and health sciences", "Cell Wall", "bakterijske celice", "Escherichia coli", "bacteria", "QD1-999", "info:eu-repo/classification/udc/579.23:577.352:544.57", "0303 health sciences", "liza celic", "Bacteria", "viability", "sonoliza", "Escherichia coli Proteins", "Acoustics. Sound", "bakterijske celice", " Escherichia coli", " celi\u010dna ovojnica", " liza celic", " ultrazvo\u010dna sonikacija", " sonoliza", "info:eu-repo/classification/udc/579", "Chemistry", "Viability"]}, "links": [{"href": "https://doi.org/10.1016/j.ultsonch.2022.105919"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ultrasonics%20Sonochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ultsonch.2022.105919", "name": "item", "description": "10.1016/j.ultsonch.2022.105919", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ultsonch.2022.105919"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2019.05.025", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:17:52Z", "type": "Journal Article", "created": "2019-05-09", "title": "Continuous ozonation of urban wastewater: Removal of antibiotics, antibiotic-resistant Escherichia coli and antibiotic resistance genes and phytotoxicity", "description": "This work evaluated the removal of a mixture of eight antibiotics (i.e. ampicillin (AMP), azithromycin (AZM), erythromycin (ERY), clarithromycin (CLA), ofloxacin (OFL), sulfamethoxazole (SMX), trimethoprim (TMP) and tetracycline (TC)) from urban wastewater, by ozonation operated in continuous mode at different hydraulic retention times (HRTs) (i.e. 10, 20, 40 and 60\u202fmin) and specific ozone doses (i.e. 0.125, 0.25, 0.50 and 0.75 gO3 gDOC- 1). As expected, the efficiency of ozonation was highly ozone dose- and contact time-dependent. The removal of the parent compounds of the selected antibiotics to levels below their detection limits was achieved with HRT of 40\u202fmin and specific ozone dose of 0.125 gO3 gDOC- 1. The effect of ozonation was also investigated at a microbiological and genomic level, by studying the efficiency of the process with respect to the inactivation of Escherichia coli and antibiotic-resistant E.\u00a0coli, as well as to the reduction of the abundance of selected antibiotic resistance genes (ARGs). The inactivation of total cultivable E.\u00a0coli was achieved under the experimental conditions of HRT 40\u202fmin and 0.25 gO3 gDOC-1, at which all antibiotic compounds were already degraded. The regrowth examinations revealed that higher ozone concentrations were required for the permanent inactivation of E.\u00a0coli below the Limit of Quantification (The effects of supplementing a tropical, low-quality grass hay (Brachiaria dictyoneura) with legume foliage (Cratylia argentea) or fruits of the multipurpose tree Sapindus saponaria on ruminal fermentation, methane release and nitrogen (N) utilization were evaluated. Six Swiss White Hill lambs were used in a 6 \uffe2\uff9c\uff95 6 Latin-square design with a 3 \uffe2\uff9c\uff95 2 factorial arrangement of treatments with measurements of energy metabolism being conducted using open-circuit respiratory chambers. Treatments consisted of three basal diets, either grass alone or legume: grass ratios of 1: 2 or 2: 1. These basal diets were supplemented (1: 3) with a control concentrate or with a concentrate containing 250 g/kg dry matter ofS. saponariafruits. The apparent total tract digestibilities of organic matter (OM) and neutral-detergent fibre (NDF) were reduced and the proportionate crude protein (CP) losses through faeces were increased (P 0\uffc2\uffb701) by supplementation withS. saponaria, and digestibilities of OM and NDF were linearly reduced (P 0\uffc2\uffb7001) with increasing legume proportion. Body energy retention, however, was similar in all diets. Along with CP intake, the proportionate CP losses through faeces decreased (P 0\uffc2\uffb7001) with increasing legume proportion which was associated with improved (P 0\uffc2\uffb7001) body protein retention and reduced (P 0\uffc2\uffb71) fat retention. Ruminal fluid ammonia concentration was not significantly affected (P > 0\uffc2\uffb71) by the inclusion ofS. saponariain the concentrate, but increased linearly (P 0\uffc2\uffb7001) as dietary legume proportion was elevated. Supplementation with fruits ofS. saponariaincreased (P 0\uffc2\uffb701) total bacteria count, and decreased (P 0\uffc2\uffb7001) total ciliate protozoa count by more than proportionately 0\uffc2\uffb750. Daily methane release was reduced (P 0\uffc2\uffb701) byS. saponariasupplementation in all basal diet types. Although being not clearly affected on a daily basis, methane release relative to body protein retention decreased linearly (P 0\uffc2\uffb705) with increasing legume proportion. The fact that interactions were mostly non-significant (P > 0\uffc2\uffb705) indicates that supplementation withS. saponariafruits is a useful means to reduce methane emission from sheep given both tropical grass-based and grass-legume-based diets. Likewise, including legumes in N-limited tropical diets seems to represent an environmentally friendly way to improve animal productivity.", "keywords": ["2. Zero hunger", "sheep", "digesti\u00f3n ruminal", "brachiaria dictyoneura", "methane", "0402 animal and dairy science", "ovinos", "saponinas", "04 agricultural and veterinary sciences", "suplementos", "feed crops", "supplements", "metano", "saponins", "sapindus saponaria", "cratylia", "rumen digestion"], "contacts": [{"organization": "Hess, HD, Beuret, RA, Lotscher, M, Hindrichsen, I.K., Machmuller, A, Carulla, Juan E., Lascano, Carlos E., Kreuzer, M.,", "roles": ["creator"]}]}, "links": [{"href": "http://www.veterinaria.unal.edu.co/inv/nutricion/Hess_2004_AS.pdf"}, {"href": "https://doi.org/10.1017/s1357729800054643"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1017/s1357729800054643", "name": "item", "description": "10.1017/s1357729800054643", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1017/s1357729800054643"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-04-01T00:00:00Z"}}, {"id": "10.1017/s1751731108001791", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:17:57Z", "type": "Journal Article", "created": "2008-04-15", "title": "Effect Of The Tropical Tannin-Rich Shrub Legumes Calliandra Calothyrsus And Flemingia Macrophylla On Methane Emission And Nitrogen And Energy Balance In Growing Lambs", "description": "Open AccessISSN:1751-7311", "keywords": ["Brachiaria; methane; ruminants; tannins; Vigna", "ruminants", "tannins", "methane", "Vigna", "0402 animal and dairy science", "Brachiaria", "04 agricultural and veterinary sciences", "SF1-1100", "Animal culture"], "contacts": [{"organization": "Tiemann, T. T., Lascano, C. E., Wettstein, H.-R, Mayer, A. C., Kreuzer, M., Hess, H. D.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1017/s1751731108001791"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Animal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1017/s1751731108001791", "name": "item", "description": "10.1017/s1751731108001791", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1017/s1751731108001791"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-01-01T00:00:00Z"}}, {"id": "10.1038/371236a0", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:18:15Z", "type": "Journal Article", "created": "2003-08-12", "title": "Carbon Storage By Introduced Deep-Rooted Grasses In The South American Savannas", "description": "ESTIMATES of the global carbon dioxide balance have identified a substantial 'missing sink' of 0.4\u20134.3 Gt per year1. It has been suggested that much of this may reside in the terrestrial biosphere2. Here we present an analysis of the carbon stored by pastures based on deep-rooted grasses which have been introduced in the South American savannas. Although the deep-rooted grasses were chosen principally for agricultural reasons3, we find that they also sequester significant amounts of organic carbon deep in the soil. If our study sites are representative of similar pastures throughout South America, this process could account for the sequestration of 100\u2013507 Mt carbon per year\u2014a substantial part of the 'missing sink'. Thus, although some land-use changes4 (such as burning tropical rainforests) contribute to the atmospheric CO2 burden, we conclude that the introduced pastures studied here help to offset the effect of anthropogenic CO2emissions.", "keywords": ["2. Zero hunger", "carb\u00f3n", "carbon", "arachis pintoi", "root systems", "04 agricultural and veterinary sciences", "15. Life on land", "feed crops", "01 natural sciences", "sistema radicular", "brachiaria humidicola", "savannas", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "andropogon gayanus", "sabanas", "stylosanthes", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1038/371236a0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/371236a0", "name": "item", "description": "10.1038/371236a0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/371236a0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1994-09-01T00:00:00Z"}}, {"id": "10.1038/ismej.2016.169", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:18:16Z", "type": "Journal Article", "created": "2017-01-03", "title": "The Pseudomonas putida T6SS is a plant warden against phytopathogens", "description": "Abstract Bacterial type VI secretion systems (T6SSs) are molecular weapons designed to deliver toxic effectors into prey cells. These nanomachines have an important role in inter-bacterial competition and provide advantages to T6SS active strains in polymicrobial environments. Here we analyze the genome of the biocontrol agent Pseudomonas putida KT2440 and identify three T6SS gene clusters (K1-, K2- and K3-T6SS). Besides, 10 T6SS effector\uffe2\uff80\uff93immunity pairs were found, including putative nucleases and pore-forming colicins. We show that the K1-T6SS is a potent antibacterial device, which secretes a toxic Rhs-type effector Tke2. Remarkably, P. putida eradicates a broad range of bacteria in a K1-T6SS-dependent manner, including resilient phytopathogens, which demonstrates that the T6SS is instrumental to empower P. putida to fight against competitors. Furthermore, we observed a drastically reduced necrosis on the leaves of Nicotiana benthamiana during co-infection with P. putida and Xanthomonas campestris. Such protection is dependent on the activity of the P. putida T6SS. Many routes have been explored to develop biocontrol agents capable of manipulating the microbial composition of the rhizosphere and phyllosphere. Here we unveil a novel mechanism for plant biocontrol, which needs to be considered for the selection of plant wardens whose mission is to prevent phytopathogen infections.
", "keywords": ["PROTEIN SECRETION", "Nicotiana", "0301 basic medicine", "570", "INTESTINAL INFLAMMATION", "05 Environmental Sciences", "VIBRIO-CHOLERAE", "Environmental Sciences & Ecology", "VI SECRETION SYSTEM", "Xanthomonas campestris", "Microbiology", "03 medical and health sciences", "Bacterial Proteins", "10 Technology", "Plant Diseases", "0303 health sciences", "Science & Technology", "Ecology", "Pseudomonas putida", "ROOT MICROBIOME", "Gene Expression Regulation", " Bacterial", "06 Biological Sciences", "Type VI Secretion Systems", "GENOMIC ANALYSIS", "Biological Control Agents", "ESCHERICHIA-COLI", "EFFECTORS", "IMMUNITY PROTEINS", "Original Article", "HOST-RANGE", "Life Sciences & Biomedicine"]}, "links": [{"href": "http://www.nature.com/articles/ismej2016169.pdf"}, {"href": "https://doi.org/10.1038/ismej.2016.169"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/ismej.2016.169", "name": "item", "description": "10.1038/ismej.2016.169", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/ismej.2016.169"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-03T00:00:00Z"}}, {"id": "10.1038/s41467-021-25665-6", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:18:19Z", "type": "Journal Article", "created": "2021-09-06", "title": "Growth-coupled selection of synthetic modules to accelerate cell factory development.", "description": "Synthetic biology has brought about a conceptual shift in our ability to redesign microbial metabolic networks. Combining metabolic pathway-modularization with growth-coupled selection schemes is a powerful tool that enables deep rewiring of the cell factories\u2019 biochemistry for rational bioproduction.", "keywords": ["0301 basic medicine", "0303 health sciences", "Science", "Q", "Comment", "Recombinant Proteins", "Biological Factors", "03 medical and health sciences", "Metabolic Engineering", "Saccharomycetales", "Escherichia coli", "Life Science", "Humans", "Synthetic Biology", "Metabolic Networks and Pathways"]}, "links": [{"href": "https://www.nature.com/articles/s41467-021-25665-6.pdf"}, {"href": "https://doi.org/10.1038/s41467-021-25665-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-021-25665-6", "name": "item", "description": "10.1038/s41467-021-25665-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-021-25665-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-09-06T00:00:00Z"}}, {"id": "10.1038/s41598-023-49194-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:18:24Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "AbstractBacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.The effects of two kinds ofEscherichia coli(E. coli) strain, wild-typeE. coliW3110 andE. colinir-Ptac, which has enhanced NO2reduction activity, on oral CH4emission and NO3toxicity in NO3-treated sheep were assessed in a respiratory hood system in a 4\uffc3\uff976 Youden square design. NO3(1\uffc2\uffb73g NaNO3/kg0\uffc2\uffb775body weight) and/orE. colistrains were delivered into the rumen through a fistula as a single dose 30min after the morning meal.Escherichia colicells were inoculated for sheep to provide an initialE. colicell density of optical density at 660nm of 2, which corresponded to 2\uffc3\uff971010cells/ml. The six treatments consisted of saline,E. coliW3110,E. colinir-Ptac, NO3, NO3plusE. coliW3110, and NO3plusE. colinir-Ptac. CH4emission from sheep was reduced by the inoculation ofE. coliW3110 orE. colinir-Ptac by 6% and 12%, respectively. NO3markedly inhibited CH4emission from sheep. Compared with sheep given NO3alone, the inoculation ofE. coliW3110 to NO3-infused sheep lessened ruminal and plasma toxic NO2accumulation and blood methaemoglobin production, while keeping ruminal methanogenesis low. Ruminal and plasma toxic NO2accumulation and blood methaemoglobin production in sheep were unaffected by the inoculation ofE. colinir-Ptac. These results suggest that ruminal methanogenesis may be reduced by the inoculation ofE. coliW3110 orE. colinir-Ptac. The inoculation ofE. coliW3110 may abate NO3toxicity when NO3is used to inhibit CH4emission from ruminants.
", "keywords": ["Male", "2. Zero hunger", "Nitrates", "Rumen", "Sheep", "Metabolic Clearance Rate", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Nitrate Reductase", "3. Good health", "Oxygen Consumption", "Fermentation", "Escherichia coli", "Animals", "Infusions", " Parenteral", "Methane", "Methemoglobin", "Nitrites"], "contacts": [{"organization": "Junichi Takahashi, I. Shinzato, Y. Asakura, C. Sar, B. Pen, R. Morikawa, B. Mwenya, A. Tsujimoto, K. Kuwaki, K. Takaura, N. Isogai, Yasuhiko Toride,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1079/bjn20051517"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/British%20Journal%20of%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1079/bjn20051517", "name": "item", "description": "10.1079/bjn20051517", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1079/bjn20051517"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-11-01T00:00:00Z"}}, {"id": "10.1111/1462-2920.15751", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:19:16Z", "type": "Journal Article", "created": "2021-09-28", "title": "Novel Alcaligenes ammonioxydans sp. nov. from wastewater treatment sludge oxidizes ammonia to N2 with a previously unknown pathway", "description": "SummaryHeterotrophic nitrifiers are able to oxidize and remove ammonia from nitrogen\uffe2\uff80\uff90rich wastewaters but the genetic elements of heterotrophic ammonia oxidation are poorly understood. Here, we isolated and identified a novel heterotrophic nitrifier, Alcaligenes ammonioxydans sp. nov. strain HO\uffe2\uff80\uff901, oxidizing ammonia to hydroxylamine and ending in the production of N2 gas. Genome analysis revealed that strain HO\uffe2\uff80\uff901 encoded a complete denitrification pathway but lacks any genes coding for homologous to known ammonia monooxygenases or hydroxylamine oxidoreductases. Our results demonstrated strain HO\uffe2\uff80\uff901 denitrified nitrite (not nitrate) to N2 and N2O at anaerobic and aerobic conditions respectively. Further experiments demonstrated that inhibition of aerobic denitrification did not stop ammonia oxidation and N2 production. A gene cluster (dnfT1RT2ABCD) was cloned from strain HO\uffe2\uff80\uff901 and enabled E. coli accumulated hydroxylamine. Sub\uffe2\uff80\uff90cloning showed that genetic cluster dnfAB or dnfABC already enabled E. coli cells to produce hydroxylamine and further to 15N2 from (15NH4)2SO4. Transcriptome analysis revealed these three genes dnfA, dnfB and dnfC were significantly upregulated in response to ammonia stimulation. Taken together, we concluded that strain HO\uffe2\uff80\uff901 has a novel dnf genetic cluster for ammonia oxidation and this dnf genetic cluster encoded a previously unknown pathway of direct ammonia oxidation (Dirammox) to N2.
", "keywords": ["Alcaligenes ammonioxydans sp. nov.", "0301 basic medicine", "106014 Genomics", "Nitrogen", "HYDROXYLAMINE OXIDASE", "direct ammonia oxidation (Dirammox)", "OXIDATION", "REDUCTASE", "Water Purification", "THIOSPHAERA-PANTOTROPHA", "PYRUVIC-OXIME", "03 medical and health sciences", "heterotrophic nitrifier", "Ammonia", "106014 Genomik", "Escherichia coli", "Alcaligenes", "wastewater", "Nitrites", "106022 Mikrobiologie", "HETEROTROPHIC NITRIFICATION", "0303 health sciences", "PURIFICATION", "Sewage", "AEROBIC DENITRIFICATION", "Nitrification", "Aerobiosis", "6. Clean water", "NITROGEN", "FAECALIS", "Denitrification", "106022 Microbiology", "Oxidation-Reduction"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15751"}, {"href": "https://doi.org/10.1111/1462-2920.15751"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.15751", "name": "item", "description": "10.1111/1462-2920.15751", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.15751"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-09-28T00:00:00Z"}}, {"id": "10.17221/559/2012-pse", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:20:39Z", "type": "Journal Article", "created": "2018-02-10", "title": "Response Of Brachiaria Grass To Selenium Forms Applied In A Tropical Soil", "description": "In Brazil the total area of native and cultivated pasture used for livestock is around 180 million hectares, and selenium (Se) is absent from mineral fertilizer formulas. Nutritional supplementation of this element takes place along with provision of mineral salts in the form of sodium selenite. In the present work, the effects of adding selenate and selenite on Se biofortification, antioxidant activity and anatomy alterations in Brachiaria brizantha were evaluated. The experiments were disposed in a completely randomized design in a 6 \u00d7 2 factorial scheme, by means of five levels of Se (0; 0.5; 1.0; 3.0 and 6.0 mg/kg) applied along with grass plant fertilizer, and two Se forms (sodium selenate and sodium selenite), with six replications. The results of the present study suggest that, in tropical soil conditions, the application of Se as selenate at low doses is more appropriate for B. brizantha biofortification than Se as selenite, because it favors a greater shoot Se levels, better activation of the antioxidant system and reduces on lipid peroxidation. Finally, with an increase of Se rates, cellular modifications were observed in internal structures of roots in B. brizantha, with aerenchyma appearing.", "keywords": ["0106 biological sciences", "2. Zero hunger", "Forage", "Brachiaria brizantha", "Root anatomy", "forage", "Plant culture", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "SB1-1110", "biofortification", "root anatomy", "antioxidant enzymes", "0401 agriculture", " forestry", " and fisheries", "Antioxidant enzymes", "brachiaria brizantha", "Biofortification"], "contacts": [{"organization": "Ramos, S\u00edlvio Junio, \u00c1vila, Fabricio William de, Boldrin, Paulo Fernandes, Pereira, Fabr\u00edcio Jos\u00e9, Castro, Evaristo Mauro de, Faquin, Valdemar, Reis, Andr\u00e9 Rodrigues dos, Guilherme, Luiz Roberto Guimar\u00e3es,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.17221/559/2012-pse"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%2C%20Soil%20and%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.17221/559/2012-pse", "name": "item", "description": "10.17221/559/2012-pse", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.17221/559/2012-pse"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-11-30T00:00:00Z"}}, {"id": "10.2134/jeq2011.0124", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-05-30T16:21:01Z", "type": "Journal Article", "created": "2012-06-29", "description": "Acid weathered soils often require lime and fertilizer application to overcome nutrient deficiencies and metal toxicity to increase soil productivity. Slow-pyrolysis chicken manure biochars, produced at 350 and 700\u00b0C with and without subsequent steam activation, were evaluated in an incubation study as soil amendments for a representative acid and highly weathered soil from Appalachia. Biochars were mixed at 5, 10, 20, and 40 g kg into a Gilpin soil (fine-loamy, mixed, active, mesic Typic Hapludult) and incubated in a climate-controlled chamber for 8 wk, along with a nonamended control and soil amended with agronomic dolomitic lime (AgLime). At the end of the incubation, soil pH, nutrient availability (by Mehlich-3 and ammonium bicarbonate diethylene triamine pentaacetic acid [AB-DTPA] extractions), and soil leachate composition were evaluated. Biochar effect on soil pH was process- and rate-dependent. Biochar increased soil pH from 4.8 to 6.6 at the high application rate (40 g kg), but was less effective than AgLime. Biochar produced at 350\u00b0C without activation had the least effect on soil pH. Biochar increased soil Mehlich-3 extractable micro- and macronutrients. On the basis of unit element applied, increase in pyrolysis temperature and biochar activation decreased availability of K, P, and S compared to nonactivated biochar produced at 350\u00b0C. Activated biochars reduced AB-DTPA extractable Al and Cd more than AgLime. Biochar did not increase NO in leachate, but increased dissolved organic carbon, total N and P, PO, SO, and K at high application rate (40 g kg). Risks of elevated levels of dissolved P may limit chicken manure biochar application rate. Applied at low rates, these biochars provide added nutritional value with low adverse impact on leachate composition.", "keywords": ["2. Zero hunger", "Appalachian Region", "Temperature", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "15. Life on land", "01 natural sciences", "6. Clean water", "Manure", "Soil", "13. Climate action", "Charcoal", "Animals", "0401 agriculture", " forestry", " and fisheries", "Chickens", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Isabel M. Lima, Harry W. Godwin, Jonathan J. Halvorson, Amir Hass, Javier M. Gonzalez, Douglas G. Boyer,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2134/jeq2011.0124"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2011.0124", "name": "item", "description": "10.2134/jeq2011.0124", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2011.0124"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-07-01T00:00:00Z"}}, {"id": "10.2134/jeq2011.0207", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-05-30T16:21:01Z", "type": "Journal Article", "created": "2012-01-05", "title": "Biochar Pyrolyzed At Two Temperatures Affects Escherichia Coli Transport Through A Sandy Soil", "description": "The incorporation of biochar into soils has been proposed as a means to sequester carbon from the atmosphere. An added environmental benefit is that biochar has also been shown to increase soil retention of nutrients, heavy metals, and pesticides. The goal of this study was to evaluate whether biochar amendments affect the transport of Escherichia coli through a water-saturated soil. We looked at the transport of three E. coli isolates through 10-cm columns packed with a fine sandy soil amended with 2 or 10% (w/w) poultry litter biochar pyrolyzed at 350 or 700\u00b0C. For all three isolates, mixing the high-temperature biochar at a rate of 2% into the soil had no impact on transport behavior. When added at a rate of 10%, a reduction of five orders of magnitude in the amount of E. coli transported through the soil was observed for two of the isolates, and a 60% reduction was observed for the third isolate. Mixing the low-temperature biochar into the soil resulted in enhanced transport through the soil for two of the isolates, whereas no significant differences in transport behavior were observed between the low-temperature and high-temperature biochar amendments for one isolate. Our results show that the addition of biochar can affect the retention and transport behavior of E. coli and that biochar application rate, biochar pyrolysis temperature, and bacterial surface characteristics were important factors determining the transport of E. coli through our test soil.", "keywords": ["2. Zero hunger", "Hot Temperature", "04 agricultural and veterinary sciences", "01 natural sciences", "6. Clean water", "3. Good health", "Soil", "13. Climate action", "Charcoal", "Escherichia coli", "Water Movements", "0401 agriculture", " forestry", " and fisheries", "Adsorption", "Water Microbiology", "Soil Microbiology", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Sergio M. Abit, Carl H. Bolster,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2134/jeq2011.0207"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2011.0207", "name": "item", "description": "10.2134/jeq2011.0207", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2011.0207"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-01-01T00:00:00Z"}}, {"id": "10.3389/fenvs.2018.00032", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:21:35Z", "type": "Journal Article", "created": "2018-05-25", "title": "Correlative Visualization of Root Mucilage Degradation Using X-ray CT and MRI", "description": "Root exudates are a crucial component of the rhizosphere. Often, they take a form of a gel exuded by the plant roots and are thought to influence the soil aggregation, root penetration into soil, soil nutrient availability, immobilization of toxic cations, and microbial activity amongst other things. In addition, the capacity of exudates to store water makes the plants potentially less susceptive to drought. Major components of root exudates are high molecular weight organic compounds consisting of predominantly polysaccharides and proteins, which makes it challenging to visualize using current rhizosphere visualization techniques, such as X-ray computed tomography (CT). In this contribution, we use correlative X-ray CT (resolution ~20 \u03bcm) in combination with Magnetic Resonance Imaging (MRI, resolution ~120 \u03bcm) to set up groundwork to enable in situ visualization of mucilage in soil. This multimodal approach is necessary because mucilage density closely matches that of water. We use chia seeds as mucilage analog, because it has been found to have a similar consistency to root mucilage. Moreover, to understand mucilage development in time, a series of samples made by chia seeds placed in different porous media were prepared. Structurally and chemically, mucilage breaks down toward a water-like substance over a course of 2 weeks. Depending on its relative concentration, these changes were found to be less dominant when seeds were mixed in porous media. Having set up the groundwork for correlative imaging of chia seeds in water and an artificial soil (Nafion and sand/beads) this enables us to expand this imaging to deal with plant root exudates under natural conditions.", "keywords": ["2. Zero hunger", "570", "X-ray CT", "chia", "polysaccharides", "04 agricultural and veterinary sciences", "15. Life on land", "630", "6. Clean water", "root-exudate", "Environmental sciences", "1H-MRI", "0401 agriculture", " forestry", " and fisheries", "GE1-350", "mucilage"]}, "links": [{"href": "https://eprints.soton.ac.uk/421014/1/NMRI_imaging_chia_review_final_2_TR_01_05_2018.pdf"}, {"href": "https://eprints.soton.ac.uk/421014/2/fenvs_06_00032.pdf"}, {"href": "https://doi.org/10.3389/fenvs.2018.00032"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fenvs.2018.00032", "name": "item", "description": "10.3389/fenvs.2018.00032", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fenvs.2018.00032"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-25T00:00:00Z"}}, {"id": "10.3390/s18072250", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:22:01Z", "type": "Journal Article", "created": "2018-07-12", "title": "Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples", "description": "Bacterial contamination of water sources (e.g., lakes, rivers and springs) from waterborne bacteria is a crucial water safety issue and its prevention is of the utmost significance since it threatens the health and well-being of wildlife, livestock, and human populations and can lead to serious illness and even death. Rapid and multiplexed measurement of such waterborne pathogens is vital and the challenge is to instantly detect in these liquid samples different types of pathogens with high sensitivity and specificity. In this work, we propose a biosensing system in which the bacteria are labelled with streptavidin coated magnetic markers (MPs\u2014magnetic particles) forming compounds (MLBs\u2014magnetically labelled bacteria). Video microscopy in combination with a particle tracking software are used for their detection and quantification. When the liquid containing the MLBs is introduced into the developed, microfluidic platform, the MLBs are accelerated towards the outlet by means of a magnetic field gradient generated by integrated microconductors, which are sequentially switched ON and OFF by a microcontroller. The velocities of the MLBs and that of reference MPs, suspended in the same liquid in a parallel reference microfluidic channel, are calculated and compared in real time by a digital camera mounted on a conventional optical microscope in combination with a particle trajectory tracking software. The MLBs will be slower than the reference MPs due to the enhanced Stokes\u2019 drag force exerted on them, resulting from their greater volume and altered hydrodynamic shape. The results of the investigation showed that the parameters obtained from this method emerged as reliable predictors for E. coli concentrations.
", "keywords": ["0301 basic medicine", "0303 health sciences", "magnetophoresis", "magnetic microparticles", "Chemical technology", "magnetic labeling", "Microfluidics", "TP1-1185", "Biosensing Techniques", "Article", "6. Clean water", "particle tracking", "Magnetics", "03 medical and health sciences", "bacteria quantification", "13. Climate action", "Escherichia coli", "biosensing", "Water Microbiology"]}, "links": [{"href": "http://www.mdpi.com/1424-8220/18/7/2250/pdf"}, {"href": "https://doi.org/10.3390/s18072250"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sensors", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/s18072250", "name": "item", "description": "10.3390/s18072250", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/s18072250"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-12T00:00:00Z"}}, {"id": "10.3897/zookeys.688.13721", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:22:10Z", "type": "Journal Article", "created": "2017-08-08", "title": "The complete mitochondrial DNA sequence of the pantropical earthworm Pontoscolex corethrurus (Rhinodrilidae, Clitellata): Mitogenome characterization and phylogenetic positioning", "description": "Pontoscolex corethrurus (M\uffc3\uffbcller, 1857) plays an important role in soil terrestrial ecosystems and has been widely used as an animal model for a large variety of ecological studies, in particular due to its common presence and generally high abundance in human-disturbed tropical soils. In this study we describe the complete mitochondrial genome of the peregrine earthworm Pontoscolex corethrurus. This is the first record of a mitochondrial genome within the Rhinodrilidae family. Its mitochondrial genome is 14 835 bp in length containing 37 genes (13 protein-coding genes (PCG), 2 rRNA genes and 22 tRNA genes). It has the same gene content and structure as in other sequenced earthworms, but unusual among invertebrates it has several overlapping open reading frames. All genes are encoded on the same strand, most of the PCGs use ATG as the start codon except for NAD3, which uses GTG as the start codon. The T+A content of the mitochondrial genome is 59.9% (31.9% A, 27.9% T, 14.9% G, and 25.3% for C). The annotated genome sequence has been deposited in GenBank under the accession number KT988053.
", "keywords": ["peregrine species", "0301 basic medicine", "Annelida", "Archiannelida", "corethrurus", "Haplotaxida", "Pontoscolex", "Eudrilidae", "03 medical and health sciences", "[SDV.EE]Life Sciences [q-bio]/Ecology", "Glossoscolecidae", "Crassiclitellata", "Animalia", "Oligochaeta", "earthworm", "Azores", "Pontoscolex corethrurus", "0303 health sciences", "500", "Cephalornis", "15. Life on land", "Rhinodrilidae", "mitochondria", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "[SDV.EE] Life Sciences [q-bio]/Ecology", " environment", "Periscolex", "QL1-991", "mitochondrial genome", "Clitellata", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "environment", "Zoology", "Research Article"]}, "links": [{"href": "https://orca.cardiff.ac.uk/id/eprint/103658/8/ZK_article_13721.pdf"}, {"href": "https://peerj.com/preprints/2847.pdf"}, {"href": "https://peerj.com/preprints/2847v1.pdf"}, {"href": "https://doi.org/10.3897/zookeys.688.13721"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ZooKeys", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3897/zookeys.688.13721", "name": "item", "description": "10.3897/zookeys.688.13721", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3897/zookeys.688.13721"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-03-03T00:00:00Z"}}, {"id": "10.5281/zenodo.14054263", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:23:35Z", "type": "Dataset", "title": "SERENA EJP Soil Green house gas aplication Moravia region, Czechia", "description": "The present dataset corresponds to a map of Net Ecosystem Productivity (NEP) for Moravia region in Czechia classified as wheat by the Eurocrop 2028 spatial product (d\u2019Andrimont et al. 2021). The map is the result of applying the NEP cookbook developed in SERENA/EJP-Soil to the area of interest (AOI) input data. NEP is expressed for a single 8-day period in early summer as it is the date with the highest value. The NEP value is a 2010-2014 average for the particular 8-day period. The data description document of the cookbook (hereafter \u201cGHG cookbook\u201d) itself corresponds to other document of the SERENA project.", "keywords": ["Czechia", "net ecosystem productivity", "Moravia-region", "GHG"], "contacts": [{"organization": "Reyes-Rojas, Jessica", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.14054263"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.14054263", "name": "item", "description": "10.5281/zenodo.14054263", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.14054263"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-11-08T00:00:00Z"}}, {"id": "10.5281/zenodo.14054264", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:23:35Z", "type": "Dataset", "title": "SERENA EJP Soil Green house gas aplication Moravia region, Czechia", "description": "The present dataset corresponds to a map of Net Ecosystem Productivity (NEP) for Moravia region in Czechia classified as wheat by the Eurocrop 2028 spatial product (d\u2019Andrimont et al. 2021). The map is the result of applying the NEP cookbook developed in SERENA/EJP-Soil to the area of interest (AOI) input data. NEP is expressed for a single 8-day period in early summer as it is the date with the highest value. The NEP value is a 2010-2014 average for the particular 8-day period. The data description document of the cookbook (hereafter \u201cGHG cookbook\u201d) itself corresponds to other document of the SERENA project.", "keywords": ["Czechia", "net ecosystem productivity", "Moravia-region", "GHG"], "contacts": [{"organization": "Reyes-Rojas, Jessica", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.14054264"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.14054264", "name": "item", "description": "10.5281/zenodo.14054264", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.14054264"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-11-08T00:00:00Z"}}, {"id": "11336/226991", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:26:08Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "AbstractBacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.Bacterial contamination of water sources (e.g., lakes, rivers and springs) from waterborne bacteria is a crucial water safety issue and its prevention is of the utmost significance since it threatens the health and well-being of wildlife, livestock, and human populations and can lead to serious illness and even death. Rapid and multiplexed measurement of such waterborne pathogens is vital and the challenge is to instantly detect in these liquid samples different types of pathogens with high sensitivity and specificity. In this work, we propose a biosensing system in which the bacteria are labelled with streptavidin coated magnetic markers (MPs\u2014magnetic particles) forming compounds (MLBs\u2014magnetically labelled bacteria). Video microscopy in combination with a particle tracking software are used for their detection and quantification. When the liquid containing the MLBs is introduced into the developed, microfluidic platform, the MLBs are accelerated towards the outlet by means of a magnetic field gradient generated by integrated microconductors, which are sequentially switched ON and OFF by a microcontroller. The velocities of the MLBs and that of reference MPs, suspended in the same liquid in a parallel reference microfluidic channel, are calculated and compared in real time by a digital camera mounted on a conventional optical microscope in combination with a particle trajectory tracking software. The MLBs will be slower than the reference MPs due to the enhanced Stokes\u2019 drag force exerted on them, resulting from their greater volume and altered hydrodynamic shape. The results of the investigation showed that the parameters obtained from this method emerged as reliable predictors for E. coli concentrations.
", "keywords": ["0301 basic medicine", "0303 health sciences", "magnetophoresis", "magnetic microparticles", "Chemical technology", "magnetic labeling", "Microfluidics", "TP1-1185", "Biosensing Techniques", "Article", "6. Clean water", "particle tracking", "Magnetics", "03 medical and health sciences", "bacteria quantification", "13. Climate action", "Escherichia coli", "biosensing", "Water Microbiology"]}, "links": [{"href": "http://www.mdpi.com/1424-8220/18/7/2250/pdf"}, {"href": "https://doi.org/2867552234"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sensors", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2867552234", "name": "item", "description": "2867552234", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2867552234"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-12T00:00:00Z"}}, {"id": "81a067f8c5ec7a73c4f671d23d2d352e", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:29:00Z", "type": "Report", "title": "Eutanasia y nazismo", "description": "Open AccessEl siguiente trabajo est\u00e1 basado en el programa \u201ceutan\u00e1sico\u201d llamado Aktion T4 impuesto en 1939 por Adolf Hitler y que se cobr\u00f3 las vidas de cientos de personas. Comienza mostrando los antecedentes hist\u00f3ricos y la fundamentaci\u00f3n ideol\u00f3gica causantes del comienzo del programa eutan\u00e1sico; contin\u00faa con las bases organizativas del programa y finaliza explicando su aplicaci\u00f3n en la sociedad alemana. El m\u00e9todo para elaborar dicho trabajo ha sido la b\u00fasqueda bibliogr\u00e1fica de art\u00edculos y obras tanto nacionales como internacionales. La principal conclusi\u00f3n de este trabajo radica en la necesidad de estudiar el pasado para evitar acontecimientos similares en el presente.", "keywords": ["Psychiatry", "Eutanasia", "Alemania", "Euthanasia", "Nazism", "Germany", "Aktion T4", "Nazismo", "Psiquiatr\u00eda"], "contacts": [{"organization": "Campos P\u00e9rez, Mar\u00eda Paz", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/81a067f8c5ec7a73c4f671d23d2d352e"}, {"rel": "self", "type": "application/geo+json", "title": "81a067f8c5ec7a73c4f671d23d2d352e", "name": "item", "description": "81a067f8c5ec7a73c4f671d23d2d352e", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/81a067f8c5ec7a73c4f671d23d2d352e"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-01-01T00:00:00Z"}}, {"id": "50|userclaim___::85c2e6974a364f4a96fdee6d82a5dd41", "type": "Feature", "geometry": null, "properties": {"license": "OPEN OPEN", "updated": "2026-05-30T16:28:14Z", "type": "Other", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "keywords": ["Bacteriocin", "Gene", "Synteny", "Microbiology", "Agricultural and Biological Sciences", "Biochemistry", " Genetics and Molecular Biology", "Genetics", "Escherichia coli", "RNA Sequencing Data Analysis", "Molecular Biology", "Biology", "GC-content", "Genome", "Acinetobacter", "Bacteria", "Probiotics and Prebiotics", "In silico", "Life Sciences", "Ribosomal RNA", "Whole genome sequencing", "FOS: Biological sciences", "Microbial Enzymes and Biotechnological Applications", "metagenomics assembly", "Biotechnology", "Food Science", "Phylogenetic tree"], "contacts": [{"organization": "Mariano Pistorio, Mar\u00eda Julia Estrella, Edgardo Jofr\u00e9, Gonzalo Torres Tejerizo, Bruno Contreras-Moreira, Daniela Medeot, Anal\u00eda Sannazzaro, Medeot, Daniela, Daniela B. Medeot, Sannazzaro, Anal\u00eda, Anal\u00eda In\u00e9s Sannazzaro, Estrella, Maria Julia, Mar\u00eda Julia Estrella, Torres Tejerizo, Gonzalo, Gonzalo Torres Tejerizo, Contreras\u2011Moreira, Bruno, Bruno Contreras\u2010Moreira, Pistorio, Mariano, Mariano Pistorio, Jofr\u00e9, Edgardo, Edgardo Jofr\u00e9,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/50|userclaim___::85c2e6974a364f4a96fdee6d82a5dd41"}, {"rel": "self", "type": "application/geo+json", "title": "50|userclaim___::85c2e6974a364f4a96fdee6d82a5dd41", "name": "item", "description": "50|userclaim___::85c2e6974a364f4a96fdee6d82a5dd41", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/50|userclaim___::85c2e6974a364f4a96fdee6d82a5dd41"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-13T00:00:00Z"}}, {"id": "PMC1608625", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:29:49Z", "type": "Journal Article", "created": "2009-04-02", "title": "Consultation-liaison scheme for self-poisoned patients in a general hospital.", "description": "In a prospective clinical trial 276 self-poisoned patients consecutively admitted to hospital were randomly allocated to medical teams or to psychiatrists for an initial psychiatric assessment and a decision about 'disposal.' Junior doctors and nurses received instruction in this work. While awaiting the outcome of the trial the randomisation was continued for 13 months and 729 allocations were made altogether. Physicians requested psychiatric opinions for roughly one in five of their patients. In other respects medical teams performed similarly to psychiatrists. Provided that due attention is given to teaching junior staff and to ensuring that psychiatric treatment and social-work support are available once patients have been assessed, such a consultation-liaison scheme could be adopted in other hospitals. This would help to change unfavourable attitudes towards self-poisoned patients and contribute to the general training of doctors and nurses.", "keywords": ["Psychiatry", "Psychotherapy", "03 medical and health sciences", "0302 clinical medicine", "England", "Poisoning", "Humans", "Education", " Medical", " Continuing", "Suicide", " Attempted", "Hospitals", " General", "Referral and Consultation", "3. Good health"], "contacts": [{"organization": "R, Gardner, R, Hanka, B, Evison, P M, Mountford, V C, O'Brien, S J, Roberts,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/PMC1608625"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/BMJ", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC1608625", "name": "item", "description": "PMC1608625", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC1608625"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1978-11-18T00:00:00Z"}}, {"id": "PMC2569841", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:29:49Z", "type": "Journal Article", "created": "2008-06-03", "title": "Transcription increases methylmethane sulfonate-induced mutations in alkB strains of Escherichia coli", "description": "Methylmethane sulfonate (MMS) produces DNA base lesions, including 3-methylcytosine (m3C), more effectively in single-stranded DNA. The repair of m3C in Escherichia coli is mediated by AlkB through oxidative demethylation and in the absence of repair, m3C leads to base-substitution mutations. We describe here results of experiments that were designed to investigate whether transcription of a gene in E. coli affects the process of mutagenesis by MMS and the roles played by AlkB and lesion bypass polymerase PolV. Using a genetic reversion assay, we have confirmed that MMS mutagenesis is suppressed by AlkB, but is enhanced by PolV. High transcription of the target gene enhances reversion frequency in an orientation-dependent manner. When the cytosines that are the likely targets of MMS were in the non-template strand (NTS), transcription increased the MMS-induced reversion frequency several fold. This increase was dependent on the presence of PolV. In contrast, when the same cytosines were present in the template strand, transcription had little effect on reversion frequency induced by MMS. These data suggest that MMS creates 3-methylcytosine adducts in the NTS and are consistent with an idea proposed previously that transcription makes the NTS transiently single-stranded and more accessible to chemicals. We propose that this is the underlying cause of its increased sensitivity to MMS and suggest that transcriptionally active DNA may be a preferred target for the action of alkylating agents that prefer single-stranded DNA.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Transcription", " Genetic", "Mutagenesis", "Escherichia coli Proteins", "Escherichia coli", "Methyl Methanesulfonate", "Mixed Function Oxygenases", "Mutagens", "3. Good health"], "contacts": [{"organization": "Douglas, Fix, Chandrika, Canugovi, Ashok S, Bhagwat,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/PMC2569841"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/DNA%20Repair", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC2569841", "name": "item", "description": "PMC2569841", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC2569841"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-08-01T00:00:00Z"}}, {"id": "PMC4683817", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:29:49Z", "type": "Journal Article", "created": "2016-04-22", "title": "Dignity- a fundamental principle of mental health care", "description": "The term \u2018Dignity\u2019 can be traced to more than 2,500 years back. \u2018Dignitas hominis\u2019 in classical Roman thought usually meant \u2018status of honor and respect\u2019 which was provided to someone only who was worthy of that honor and respect because of a particular status that he or she had (e.g. because of a specific public position)1. Afterwards, the concept has been influenced by religions, philosophies across hundreds of years until, it was introduced into legal frameworks in twentieth century through the Universal Declaration of Human Rights (UDHR) in its first article which enshrined the notion of universality of human dignity in international law, by stating that, \u201cAll human beings are born free and equal in dignity and rights\u201d. Article 25 of UDHR has stated that, \u201cEveryone has the right to a standard of living adequate for the health and well-being.., including\u2026. medical care and necessary social services\u201d2. The UDHR has contributed in establishing a moral grounding for improved standards of care on the basis of our basic responsibilities towards each other as equal members of \u201chumanity\u201d3. The concept of \u2018universality of dignity\u2019 has also been reflected in several other international treaties and conventions including, most recently, the Convention on Rights of People with Disabilities4. The UK's Royal College of Nursing provides a useful definition of dignity in relation to nursing care, which can be applied to the health care field more generally as \u201cDignity is concerned with how people feel, think and behave in relation to the worth or value of themselves and others. To treat someone with dignity is to treat them as being of worth, in a way that is respectful of them as valued individuals\u201d. The definition identifies two dimensions of dignity:first is the self-worth as perceived by the individual and the second is the worth of the individual which is reflected in the respectful attitudes and practices of others5. All around the world, many people with mental disorders are deprived of their right to be treated as valued individuals. In fact, they are not only marginalized in their communities but they are also subject to wide ranging forms of human rights violations in treatment facilities.", "keywords": ["Personhood", "03 medical and health sciences", "Editorial", "Mental Health", "0302 clinical medicine", "Human Rights", "Humans", "Psychiatric Nursing", "10. No inequality", "16. Peace & justice", "3. Good health"], "contacts": [{"organization": "Saxena, Shekhar, Hanna, Fahmy,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/PMC4683817"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Indian%20Journal%20of%20Medical%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC4683817", "name": "item", "description": "PMC4683817", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC4683817"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-01T00:00:00Z"}}, {"id": "PMC6068504", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:29:50Z", "type": "Journal Article", "created": "2018-07-12", "title": "Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples", "description": "Bacterial contamination of water sources (e.g., lakes, rivers and springs) from waterborne bacteria is a crucial water safety issue and its prevention is of the utmost significance since it threatens the health and well-being of wildlife, livestock, and human populations and can lead to serious illness and even death. Rapid and multiplexed measurement of such waterborne pathogens is vital and the challenge is to instantly detect in these liquid samples different types of pathogens with high sensitivity and specificity. In this work, we propose a biosensing system in which the bacteria are labelled with streptavidin coated magnetic markers (MPs\u2014magnetic particles) forming compounds (MLBs\u2014magnetically labelled bacteria). Video microscopy in combination with a particle tracking software are used for their detection and quantification. When the liquid containing the MLBs is introduced into the developed, microfluidic platform, the MLBs are accelerated towards the outlet by means of a magnetic field gradient generated by integrated microconductors, which are sequentially switched ON and OFF by a microcontroller. The velocities of the MLBs and that of reference MPs, suspended in the same liquid in a parallel reference microfluidic channel, are calculated and compared in real time by a digital camera mounted on a conventional optical microscope in combination with a particle trajectory tracking software. The MLBs will be slower than the reference MPs due to the enhanced Stokes\u2019 drag force exerted on them, resulting from their greater volume and altered hydrodynamic shape. The results of the investigation showed that the parameters obtained from this method emerged as reliable predictors for E. coli concentrations.
", "keywords": ["0301 basic medicine", "0303 health sciences", "magnetophoresis", "magnetic microparticles", "Chemical technology", "magnetic labeling", "Microfluidics", "TP1-1185", "Biosensing Techniques", "Article", "6. Clean water", "particle tracking", "Magnetics", "03 medical and health sciences", "bacteria quantification", "13. Climate action", "Escherichia coli", "biosensing", "Water Microbiology"]}, "links": [{"href": "http://www.mdpi.com/1424-8220/18/7/2250/pdf"}, {"href": "https://doi.org/PMC6068504"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sensors", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC6068504", "name": "item", "description": "PMC6068504", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC6068504"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-12T00:00:00Z"}}, {"id": "85c2e6974a364f4a96fdee6d82a5dd41", "type": "Feature", "geometry": null, "properties": {"license": "OPEN OPEN", "updated": "2026-05-30T16:29:05Z", "type": "Other", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "keywords": ["Bacteriocin", "Gene", "Synteny", "Microbiology", "Agricultural and Biological Sciences", "Biochemistry", " Genetics and Molecular Biology", "Genetics", "Escherichia coli", "RNA Sequencing Data Analysis", "Molecular Biology", "Biology", "GC-content", "Genome", "Acinetobacter", "Bacteria", "Probiotics and Prebiotics", "In silico", "Life Sciences", "Ribosomal RNA", "Whole genome sequencing", "FOS: Biological sciences", "Microbial Enzymes and Biotechnological Applications", "metagenomics assembly", "Biotechnology", "Food Science", "Phylogenetic tree"], "contacts": [{"organization": "Mariano Pistorio, Mar\u00eda Julia Estrella, Edgardo Jofr\u00e9, Gonzalo Torres Tejerizo, Bruno Contreras-Moreira, Daniela Medeot, Anal\u00eda Sannazzaro, Medeot, Daniela, Daniela B. Medeot, Sannazzaro, Anal\u00eda, Anal\u00eda In\u00e9s Sannazzaro, Estrella, Maria Julia, Mar\u00eda Julia Estrella, Torres Tejerizo, Gonzalo, Gonzalo Torres Tejerizo, Contreras\u2011Moreira, Bruno, Bruno Contreras\u2010Moreira, Pistorio, Mariano, Mariano Pistorio, Jofr\u00e9, Edgardo, Edgardo Jofr\u00e9,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/85c2e6974a364f4a96fdee6d82a5dd41"}, {"rel": "self", "type": "application/geo+json", "title": "85c2e6974a364f4a96fdee6d82a5dd41", "name": "item", "description": "85c2e6974a364f4a96fdee6d82a5dd41", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/85c2e6974a364f4a96fdee6d82a5dd41"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-13T00:00:00Z"}}, {"id": "1c2af514-cc4f-4c16-b452-b38ecd952a3a", "type": "Feature", "geometry": null, "properties": {"themes": [{"concepts": [{"id": "environment"}], "scheme": "https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_TopicCategoryCode"}, {"concepts": [], "scheme": "https://rod.eionet.europa.eu/obligations"}, {"concepts": [{"id": "2011 1.1.1"}], "scheme": "https://applications.eea.europa.eu/ManagementPlan/default.aspx#"}, {"concepts": [{"id": "Industry"}, {"id": "Air pollution"}], "scheme": "https://www.eea.europa.eu/themes"}, {"concepts": [], "scheme": "http://geonetwork-opensource.org/gemet"}, {"concepts": [{"id": "Lithuania"}, {"id": "Slovakia"}, {"id": "Finland"}, {"id": "Malta"}, {"id": "Cyprus"}, {"id": "Latvia"}, {"id": "Norway"}, {"id": "Germany"}, {"id": "United Kingdom"}, {"id": "Portugal"}, {"id": "Austria"}, {"id": "Slovenia"}, {"id": "Iceland"}, {"id": "Liechtenstein"}, {"id": "Belgium"}, {"id": "Switzerland"}, {"id": "Italy"}, {"id": "Greece"}, {"id": "Poland"}, {"id": "France"}, {"id": "Bulgaria"}, {"id": "Netherlands"}, {"id": "Romania"}, {"id": "Hungary"}, {"id": "Denmark"}, {"id": "Luxembourg"}, {"id": "Estonia"}, {"id": "Spain"}, {"id": "Sweden"}, {"id": "Czechia"}, {"id": "Ireland"}], "scheme": "http://geonetwork-opensource.org/thesaurus/naturalearth-and-seavox"}], "license": "No limitations to public access", "rights": "License CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/). Copyright holder: Directorate-General for Environment (DG ENV).", "updated": "2026-02-09T18:09:34.756653Z", "type": "Dataset", "created": "2016-09-14T11:02:41+00:00", "language": "eng", "title": "Diffuse Air Releases (Art 8) under the European Pollutant Release and Transfer Register (E-PRTR) Regulation", "description": "The European Pollutant Release and Transfer Register (E-PRTR) is a web-based register established by Regulation (EC) No 166/2006 which implements the UNECE PRTR Protocol.", "formats": [{"name": "GeoTIFF"}, {"name": "EEA:FOLDERPATH"}, {"name": "WWW:URL"}], "keywords": ["2011 1.1.1", "Industry", "Air pollution", "Lithuania", "Slovakia", "Finland", "Malta", "Cyprus", "Latvia", "Norway", "Germany", "United Kingdom", "Portugal", "Austria", "Slovenia", "Iceland", "Liechtenstein", "Belgium", "Switzerland", "Italy", "Greece", "Poland", "France", "Bulgaria", "Netherlands", "Romania", "Hungary", "Denmark", "Luxembourg", "Estonia", "Spain", "Sweden", "Czechia", "Ireland"], "contacts": [{"name": null, "organization": "European Environment Agency", "position": null, "roles": ["pointOfContact"], "phones": [{"value": null}], "emails": [{"value": null}], "addresses": [{"deliveryPoint": ["Kongens Nytorv 6"], "city": "Copenhagen", "administrativeArea": "K", "postalCode": "1050", "country": "Denmark"}], "links": [{"href": null}]}, {"name": null, "organization": "European Environment Agency", "position": null, "roles": ["publisher"], "phones": [{"value": null}], "emails": [{"value": null}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": "http://www.eea.europa.eu#organization", "protocol": null, "protocol_url": "", "name": null, "name_url": "", "description": null, "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": null, "organization": "Directorate-General for Environment (DG ENV)", "position": null, "roles": ["processor"], "phones": [{"value": null}], "emails": [{"value": null}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": "http://ec.europa.eu/dgs/environment/index_en.htm", "protocol": null, "protocol_url": "", "name": null, "name_url": "", "description": null, "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}], "denominator": "1000000", "distanceuom": "m"}, "links": [{"href": "https://sdi.eea.europa.eu/webdav/datastore/public/eea_v_4326_1_mio_diffuse-air-releases-e-prtr_p_2008_v01_r00", "protocol": "EEA:FOLDERPATH", "rel": "download"}, {"href": "https://sdi.eea.europa.eu/data/1c2af514-cc4f-4c16-b452-b38ecd952a3a", "name": "Direct download", "description": "The set of 32 maps shows the distribution of air pollutant emissions from diffuse sources. The data is based on a scale of 5 by 5 km grid and includes details of nitrogen oxides (NOx), sulphur oxides (SOx), carbon monoxide (CO), ammonia (NH3), carbon dioxide (CO2) and particulate matter (PM10). It complements existing data on emissions from individual industrial plants included in the European Pollutant Release and Transfer Register (E-PRTR).", "protocol": "WWW:URL", "rel": "download"}, {"href": "https://sdi.eea.europa.eu/catalogue/srv/api/records/1c2af514-cc4f-4c16-b452-b38ecd952a3a/attachments/EPRTR_thumbnail.png", "name": "preview", "description": "Web image thumbnail (URL)", "protocol": "WWW:LINK-1.0-http--image-thumbnail", "rel": "preview"}, {"rel": "self", "type": "application/geo+json", "title": "1c2af514-cc4f-4c16-b452-b38ecd952a3a", "name": "item", "description": "1c2af514-cc4f-4c16-b452-b38ecd952a3a", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1c2af514-cc4f-4c16-b452-b38ecd952a3a"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"interval": ["2008-05-30T00:00:00Z", "2008-05-30T00:00:00Z"]}}, {"id": "81754d01-8bc3-49aa-a52c-86b3d212f94e", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[-14.0, 35.0], [-14.0, 72.0], [41.0, 72.0], [41.0, 35.0], [-14.0, 35.0]]]}, "properties": {"themes": [{"concepts": [{"id": "environment"}], "scheme": "https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_TopicCategoryCode"}, {"concepts": [{"id": "Habitats and biotopes"}, {"id": "Species distribution"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}, {"concepts": [{"id": "forest policy"}, {"id": "primary forest"}, {"id": "environmental indicator"}, {"id": "conservation"}, {"id": "forest conservation"}, {"id": "forest"}, {"id": "forest ecosystem"}, {"id": "indigenous forest"}], "scheme": "GEMET"}, {"concepts": [{"id": "Poland"}, {"id": "Malta"}, {"id": "Bulgaria"}, {"id": "Slovakia"}, {"id": "Slovenia"}, {"id": "Romania"}, {"id": "Lithuania"}, {"id": "Czechia"}, {"id": "Estonia"}, {"id": "Latvia"}, {"id": "Hungary"}, {"id": "EU15"}], "scheme": "Continents, countries, sea regions of the world."}, {"concepts": [{"id": "European"}], "scheme": "http://inspire.ec.europa.eu/metadata-codelist/SpatialScope"}, {"concepts": [{"id": "2019 1.7.8"}], "scheme": "EEA Management Plan"}, {"concepts": [{"id": "Biodiversity"}, {"id": "Forests and forestry"}, {"id": "Nature protection and restoration"}], "scheme": "EEA topics"}], "license": "no limitations to public access", "rights": "License CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/). Copyright holder: European Environment Agency (EEA).", "updated": "2025-10-09T11:00:27.48536Z", "type": "Dataset", "created": "2018-11-30", "language": "eng", "title": "Richness of forest-related species and habitats indicator 2012 dataset, Nov. 2018", "description": "This dataset refers to the Richness index of Species and Habitats of Conservation Concern indicator. This indicator has been developed to be used as a sub-indicator for contributing to the identification of the High Nature Value (HNV) Forest Areas as it will be integrated with other sub-indicators of horizontal structure, management and naturalness to generate the final composite indicator. It is composed itself of three sub-indicators: \u201cForest Non-bird species\u201d, \u201cForest bird species\u201d and \u201cForest habitats\u201d. All the three sub-indicators build on distribution data from the reporting of habitat and species conservation status under Article 17 of the Habitats Directive and Article 12 of the Birds directive which describe their distribution at 10km grid resolution. The forest species and the forest habitats proposed to be used for the HNV forest area identification were selected based on expert judgement (ETC/BD) and raster files reporting the count of forest species and habitats were created. At this stage, no weight is applied based on Habitat and Species prioritization, conservation status or endemism. \nThe sub-indicators were then normalized for each European forest type and successively combined not assigning any specific weight to a particular sub-indicator.\nThe values for this indicator, present in this dataset, ranges between 0 and 1. The values close to 1 mean high presence of habitats and species related to forest, whereas the lower richness are closer to 0. It covers the forested areas of the EU 27 (2007) Member States except for Cyprus (data from Croatia will be reported starting from the next update regarding the period 2013-2018).", "formats": [{"name": "GeoTIFF"}, {"name": "EEA:FOLDERPATH"}, {"name": "WWW:URL"}, {"name": "ESRI:REST"}, {"name": "OGC:WMS"}], "keywords": ["Habitats and biotopes", "Species distribution", "forest policy", "primary forest", "environmental indicator", "conservation", "forest conservation", "forest", "forest ecosystem", "indigenous forest", "Poland", "Malta", "Bulgaria", "Slovakia", "Slovenia", "Romania", "Lithuania", "Czechia", "Estonia", "Latvia", "Hungary", "EU15", "European", "2019 1.7.8", "Biodiversity", "Forests and forestry", "Nature protection and restoration"], "contacts": [{"name": null, "organization": "European Environment Agency", "position": null, "roles": ["pointOfContact"], "phones": [{"value": null}], "emails": [{"value": "sdi@eea.europa.eu"}], "addresses": [{"deliveryPoint": ["Kongens Nytorv 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UrbClim (De Ridder et al., 2015). 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