{"type": "FeatureCollection", "features": [{"id": "10.1002/ecs2.2226", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:14:28Z", "type": "Journal Article", "created": "2018-05-31", "title": "Mycorrhiza in tree diversity-ecosystem function relationships: conceptual framework and experimental implementation", "description": "Abstract

The widely observed positive relationship between plant diversity and ecosystem functioning is thought to be substantially driven by complementary resource use of plant species. Recent work suggests that biotic interactions among plants and between plants and soil organisms drive key aspects of resource use complementarity. Here, we provide a conceptual framework for integrating positive biotic interactions across guilds of organisms, more specifically between plants and mycorrhizal types, to explain resource use complementarity in plants and its consequences for plant competition. Our overarching hypothesis is that ecosystem functioning increases when more plant species associate with functionally dissimilar mycorrhizal fungi because differing mycorrhizal types will increase coverage of habitat space for and reduce competition among plants. We introduce a recently established field experiment (MyDiv) that uses different pools of tree species that associate with either arbuscular or ectomycorrhizal fungi to create orthogonal experimental gradients in tree species richness and mycorrhizal associations and present initial results. Finally, we discuss options for future mechanistic studies on resource use complementarity within MyDiv. We show how mycorrhizal types and biotic interactions in MyDiv can be used in the future to test novel questions regarding the mechanisms underlying biodiversity\uffe2\uff80\uff93ecosystem function relationships.

", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "biodiversity\u2013ecosystem functioning", "experimental design", "Ecology", "arbuscular mycorrhiza", "15. Life on land", "01 natural sciences", "ectomycorrhiza", "Article", "biotic interactions", "03 medical and health sciences", "biodiversity effects", "QH540-549.5"]}, "links": [{"href": "https://esajournals.onlinelibrary.wiley.com/doi/pdf/10.1002/ecs2.2226"}, {"href": "https://doi.org/10.1002/ecs2.2226"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecs2.2226", "name": "item", "description": "10.1002/ecs2.2226", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecs2.2226"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-01T00:00:00Z"}}, {"id": "10.1016/j.foreco.2022.120608", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:16:50Z", "type": "Journal Article", "created": "2022-11-01", "title": "Tree species traits and mycorrhizal association shape soil microbial communities via litter quality and species mediated soil properties", "description": "Open AccessLes sols abritent une grande diversit\u00e9 de microbiote du sol, qui jouent un r\u00f4le crucial dans les processus \u00e9cosyst\u00e9miques cl\u00e9s tels que la transformation de la liti\u00e8re et la min\u00e9ralisation, mais la fa\u00e7on dont les interactions complexes plante-sol fa\u00e7onnent la diversit\u00e9 et la composition du microbiote du sol reste insaisissable. Nous avons effectu\u00e9 le s\u00e9quen\u00e7age de l'amplicon de l'ADN isol\u00e9 \u00e0 partir de la couche arable min\u00e9rale de six arbres europ\u00e9ens communs plant\u00e9s dans des peuplements de monoculture de jardins communs multi-sites d'\u00e9rables \u00e0 feuilles larges et de fr\u00eanes associ\u00e9s \u00e0 des mycorhizes arbusculaires (MA), de h\u00eatres \u00e0 feuilles larges, de chaux et de ch\u00eanes associ\u00e9s \u00e0 des champignons ectomycorhiziens (MCE) et d'\u00e9pinettes de conif\u00e8res associ\u00e9es \u00e0 la MCE. L'objectif principal de cette \u00e9tude \u00e9tait d'\u00e9valuer les effets de l'identit\u00e9 des esp\u00e8ces d'arbres, des traits et des associations mycorhiziennes sur la diversit\u00e9, la structure de la communaut\u00e9, la coh\u00e9sion et le changement dans l'abondance relative des groupes taxonomiques et fonctionnels de bact\u00e9ries, de champignons et de n\u00e9matodes du sol. Nos r\u00e9sultats ont r\u00e9v\u00e9l\u00e9 que les sols sous les feuillus abritaient une plus grande richesse en bact\u00e9ries, champignons et n\u00e9matodes que sous l'\u00e9pinette de Norv\u00e8ge. Les esp\u00e8ces d'arbres \u00e0 feuilles larges associ\u00e9es aux champignons de la MA ont montr\u00e9 une plus grande coh\u00e9sion des communaut\u00e9s bact\u00e9riennes et fongiques que les arbres \u00e0 feuilles larges associ\u00e9s aux champignons de la mec, mais la coh\u00e9sion des communaut\u00e9s de n\u00e9matodes \u00e9tait plus \u00e9lev\u00e9e sous les arbres associ\u00e9s aux champignons de la mec que sous les arbres associ\u00e9s aux champignons de la MA. Les bact\u00e9ries copiotrophes, les saprotrophes fongiques et les n\u00e9matodes bact\u00e9rivores \u00e9taient associ\u00e9s au fr\u00eane, \u00e0 l'\u00e9rable et \u00e0 la chaux ayant un pH du sol \u00e9lev\u00e9 et des indices de d\u00e9composition de la liti\u00e8re \u00e9lev\u00e9s, tandis que les bact\u00e9ries oligotrophes, les champignons ectomycorhiziens et les n\u00e9matodes fongivores \u00e9taient associ\u00e9s au h\u00eatre, au ch\u00eane et \u00e0 l'\u00e9pinette de Norv\u00e8ge qui avaient un pH du sol faible et des indices de d\u00e9composition de la liti\u00e8re faibles. Les esp\u00e8ces d'arbres associ\u00e9es aux champignons AM pr\u00e9sentaient une forte proportion de bact\u00e9ries copiotrophes et de champignons saprotrophes, tandis que les arbres associ\u00e9s aux champignons ECM pr\u00e9sentaient une abondance relative \u00e9lev\u00e9e de bact\u00e9ries oligotrophes, de champignons ECM et de n\u00e9matodes fongivores. Les diff\u00e9rentes abondances de ces groupes fonctionnels soutiennent l'\u00e9conomie nutritive plus inorganique des esp\u00e8ces d'arbres AM par rapport \u00e0 l'\u00e9conomie nutritive plus organique des esp\u00e8ces d'arbres ECM. La communaut\u00e9 bact\u00e9rienne a \u00e9t\u00e9 indirectement affect\u00e9e par la qualit\u00e9 de la liti\u00e8re via les propri\u00e9t\u00e9s du sol, tandis que la communaut\u00e9 fongique a \u00e9t\u00e9 directement affect\u00e9e par la qualit\u00e9 de la liti\u00e8re et les esp\u00e8ces d'arbres. Les groupes fonctionnels des n\u00e9matodes refl\u00e9taient les communaut\u00e9s de bact\u00e9ries et de champignons, indiquant ainsi les groupes principaux et actifs des communaut\u00e9s microbiennes sp\u00e9cifiques aux esp\u00e8ces d'arbres. Notre \u00e9tude a sugg\u00e9r\u00e9 que l'identit\u00e9, les traits et l'association mycorhizienne des esp\u00e8ces d'arbres fa\u00e7onnent consid\u00e9rablement les communaut\u00e9s microbiennes via un effet direct de la chimie de la liti\u00e8re ainsi que via les propri\u00e9t\u00e9s du sol m\u00e9di\u00e9es par la liti\u00e8re.", "keywords": ["Fagus sylvatica", "Soil Science", "Plant Science", "Plant litter", "Agricultural and Biological Sciences", "Soil biology", "Mycorrhizal Fungi and Plant Interactions", "Soil water", "Genetics", "Saproxylic Insect Ecology and Forest Management", "Soil microbiota", "Symbiosis", "Plant Interactions", "Biology", "Ecosystem", "Amplicon sequencing", "Beech", "Ecology", "Bacteria", "Common garden experiment", "Botany", "Life Sciences", "04 agricultural and veterinary sciences", "15. Life on land", "Ectomycorrhiza", "Insect Science", "FOS: Biological sciences", "Functional groups", "Community cohesion", "0401 agriculture", " forestry", " and fisheries", "Trophic interactions", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "Mycorrhiza"]}, "links": [{"href": "https://doi.org/10.1016/j.foreco.2022.120608"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forest%20Ecology%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.foreco.2022.120608", "name": "item", "description": "10.1016/j.foreco.2022.120608", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.foreco.2022.120608"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10.1007/s00572-015-0655-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:15:03Z", "type": "Journal Article", "created": "2015-07-25", "title": "The Ectomycorrhizal Community Of Conifer Stands On Peat Soils 12 Years After Fertilization With Wood Ash", "description": "We studied long-term effects of fertilization with wood ash on biomass, vitality and mycorrhizal colonization of fine roots in three conifer forest stands growing in Vacciniosa turf. mel. (V), Myrtillosa turf. mel. (M) and Myrtillosa turf. mel./Caricoso-phragmitosa (MC) forest types on peat soils. Fertilization trials amounting 5 kg/m(2) of wood ash were established 12 years prior to this study. A total of 63 soil samples with roots were collected and analysed. Ectomycorrhizal (ECM) fungi in roots were identified by morphotyping and sequencing of the fungal internal transcribed spacer (ITS) region. In all forest types, fine root biomass was higher in fertilized plots than in control plots. In M forest type, proportion of living fine roots was greater in fertilized plots than in control plots, while in V and MC, the result was opposite. Fifty ECM species were identified, of which eight were common to both fertilized and control plots. Species richness and Shannon diversity index were generally higher in fertilized plots than in control plots. The most common species in fertilized plots were Amphinema byssoides (17.8%) and Tuber cf. anniae (12.2%), while in control plots, it was Tylospora asterophora (18.5%) and Lactarius tabidus (20.3%). Our results showed that forest fertilization with wood ash has long-lasting effect on diversity and composition of ECM fungal communities.", "keywords": ["0106 biological sciences", "570", "forest fertilization", "m\u00e4nty", "Molecular Sequence Data", "organic soils", "fine roots", "Plant Roots", "01 natural sciences", "630", "mets\u00e4nlannoitus", "Mycorrhizae", "ectomycorrhizae", "DNA", " Ribosomal Spacer", "Muut aihealueet", "DNA", " Fungal", "2. Zero hunger", "Picea abies", "Pinus sylvestris", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "15. Life on land", "Biota", "hienojuuret", "kuusi", "Tracheophyta", "eloper\u00e4iset maat", "0401 agriculture", " forestry", " and fisheries", "ektomykorritsa"]}, "links": [{"href": "https://doi.org/10.1007/s00572-015-0655-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00572-015-0655-2", "name": "item", "description": "10.1007/s00572-015-0655-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00572-015-0655-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-07-26T00:00:00Z"}}, {"id": "10.1016/j.ejsobi.2010.04.003", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:16:31Z", "type": "Journal Article", "created": "2010-04-26", "title": "Seasonal Dynamics Of The Physicochemical And Biological Properties Of Soils In Naturally Regenerating, Unmanaged And Clear-Cut Beech Stands In Northern Spain", "description": "The physicochemical and biological properties of soils within an unmanaged beech stand and two stands clear-cut in 2001 or 1996 were studied and compared across the year 2008. The clear-cut stands were left to naturally regenerate and exhibited very different levels of tree density. Soil from the stand clear-cut in 2001 had the lowest contents of organic matter and nitrogen, showed high resistance to penetration and the pH varied throughout the seasons. Basal respiration achieved minimum values in summer in both the unmanaged stand and the stand clear-cut in 1996. However, basal respiration slightly fluctuated from spring to autumn in the stand clear-cut in 2001. The seasonal dynamics of protease and phosphatase activities were similar within the three stands: the maximum protease activity was detected in spring and the highest phophatase activity in winter. \u03b2-Glucosidase activity in autumn and dehydrogenase in winter were greater in the unmanaged than in the clear-cut stands. Moreover, dehydrogenase activity was extremely low in the stand clear-cut in 1996. Microclimatic parameters within the stands were significantly correlated with several biological properties of soils, with microclimate being strongly determined by the density of trees. Results also suggested that ectomycorrhizal fungi would be key components of the soil microflora in the beech forests.", "keywords": ["0106 biological sciences", "Clear-cutting", "Beech forests", "0401 agriculture", " forestry", " and fisheries", "Ectomycorrhizas", "Soil basal respiration", "04 agricultural and veterinary sciences", "Soil enzymatic activities", "15. Life on land", "01 natural sciences", "Soil physicochemical properties"]}, "links": [{"href": "https://doi.org/10.1016/j.ejsobi.2010.04.003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ejsobi.2010.04.003", "name": "item", "description": "10.1016/j.ejsobi.2010.04.003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ejsobi.2010.04.003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-05-01T00:00:00Z"}}, {"id": "10.1038/s41467-019-11993-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:18:19Z", "type": "Journal Article", "created": "2019-09-04", "title": "Plant roots increase both decomposition and stable organic matter formation in boreal forest soil", "description": "Abstract

Boreal forests are ecosystems with low nitrogen (N) availability that store globally significant amounts of carbon (C), mainly in plant biomass and soil organic matter (SOM). Although crucial for future climate change predictions, the mechanisms controlling boreal C and N pools are not well understood. Here, using a three-year field experiment, we compare SOM decomposition and stabilization in the presence of roots, with exclusion of roots but presence of fungal hyphae and with exclusion of both roots and fungal hyphae. Roots accelerate SOM decomposition compared to the root exclusion treatments, but also promote a different soil N economy with higher concentrations of organic soil N compared to inorganic soil N accompanied with the build-up of stable SOM-N. In contrast, root exclusion leads to an inorganic soil N economy (i.e., high level of inorganic N) with reduced stable SOM-N build-up. Based on our findings, we provide a framework on how plant roots affect SOM decomposition and stabilization.

", "keywords": ["roots", "0106 biological sciences", "330", "Nitrogen", "Science", "ta1171", "Hyphae", "Models", " Biological", "Plant Roots", "01 natural sciences", "Article", "LITTER DECOMPOSITION", "Soil", "POLYPHENOLS", "CARBON SEQUESTRATION", "soil organic matter", "Taiga", "SDG 13 - Climate Action", "SUGAR MAPLE", "Biomass", "Organic Chemicals", "forest ecology", "106026 Ecosystem research", "Ecosystem", "Soil Microbiology", "TANNINS", "2. Zero hunger", "106022 Mikrobiologie", "ECTOMYCORRHIZAL FUNGI", "MYCORRHIZA", "Q", "ta1182", "Forestry", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "Carbon", "Environmental sciences", "NITROGEN", "Boreal forests", "106026 \u00d6kosystemforschung", "13. Climate action", "SDG 13 \u2013 Ma\u00dfnahmen zum Klimaschutz", "106022 Microbiology", "ta1181", "0401 agriculture", " forestry", " and fisheries", "COMMUNITIES", "STORAGE"]}, "links": [{"href": "https://www.nature.com/articles/s41467-019-11993-1.pdf"}, {"href": "https://doi.org/10.1038/s41467-019-11993-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-019-11993-1", "name": "item", "description": "10.1038/s41467-019-11993-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-019-11993-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-09-04T00:00:00Z"}}, {"id": "10.1093/femsec/fiz133", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:19:00Z", "type": "Journal Article", "created": "2019-08-22", "title": "Restriction of plant roots in boreal forest organic soils affects the microbial community but does not change the dominance from ectomycorrhizal to saprotrophic fungi", "description": "ABSTRACT

Boreal forest soils store significant amounts of carbon and are cohabited by saprotrophic and ectomycorrhizal fungi (ECM). The \uffe2\uff80\uff98Gadgil effect\uffe2\uff80\uff99 implies antagonistic interactions between saprotrophic fungi and ECM. Plant photosynthates support the competitive fitness of the ECM, and may also shape the soil bacterial communities. Many \uffe2\uff80\uff98Gadgil effect\uffe2\uff80\uff99 experiments have focused on litter layer (OL) or have litter and root-fragments present, and thus possibly favor the saprotrophs. We compared how the restriction of plant roots and exudates affect soil microbial community structures in organic soil (mixed OF and OH). For this, we established a 3-yr field experiment with 3 different mesh treatments affecting the penetration of plant roots and external fungal hyphae. Exclusion of plant photosynthates induced modest changes in both fungal and bacterial community structures, but not to potential functionality of the microbial community. The microbial community was resilient towards rather short-term disturbances. Contrary to the \uffe2\uff80\uff98Gadgil effect\uffe2\uff80\uff99, mesh treatments restricting the entrance of plant roots and external fungal hyphae did not favor saprotrophs that originally inhabited the soil. Thus, we propose that different substrate preferences (fresh litter vs. fermented or humified soil), rather than antagonism, maintain the spatial separation of saprotrophs and mycorrhizal fungi in boreal forest soils.

", "keywords": ["0301 basic medicine", "570", "Hyphae", "577", "Plant Roots", "ectomycorrhiza", "Trees", "Soil", "03 medical and health sciences", "boreal forest soil", "Mycorrhizae", "Taiga", "saprotrophs", "Soil Microbiology", "2. Zero hunger", "0303 health sciences", "Microbiota", "Fungi", "Plants", "15. Life on land", "Gadgil effect", "Carbon", "functional gene profile", "13. Climate action", "ta1181", "microbial community"]}, "links": [{"href": "http://academic.oup.com/femsec/article-pdf/95/9/fiz133/29808832/fiz133.pdf"}, {"href": "https://doi.org/10.1093/femsec/fiz133"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiz133", "name": "item", "description": "10.1093/femsec/fiz133", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiz133"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-23T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2008.01549.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:19:34Z", "type": "Journal Article", "created": "2008-02-11", "title": "Microbial Activity And Soil Respiration Under Nitrogen Addition In Alaskan Boreal Forest", "description": "Abstract

Climate warming could increase rates of soil organic matter turnover and nutrient mineralization, particularly in northern high\uffe2\uff80\uff90latitude ecosystems. However, the effects of increasing nutrient availability on microbial processes in these ecosystems are poorly understood. To determine how soil microbes respond to nutrient enrichment, we measured microbial biomass, extracellular enzyme activities, soil respiration, and the community composition of active fungi in nitrogen (N) fertilized soils of a boreal forest in central Alaska. We predicted that N addition would suppress fungal activity relative to bacteria, but stimulate carbon (C)\uffe2\uff80\uff90degrading enzyme activities and soil respiration. Instead, we found no evidence for a suppression of fungal activity, although fungal sporocarp production declined significantly, and the relative abundance of two fungal taxa changed dramatically with N fertilization. Microbial biomass as measured by chloroform fumigation did not respond to fertilization, nor did the ratio of fungi\uffe2\uff80\uff83:\uffe2\uff80\uff83bacteria as measured by quantitative polymerase chain reaction. However, microbial biomass C\uffe2\uff80\uff83:\uffe2\uff80\uff83N ratios narrowed significantly from 16.0 \uffc2\uffb1 1.4 to 5.2 \uffc2\uffb1 0.3 with fertilization. N fertilization significantly increased the activity of a cellulose\uffe2\uff80\uff90degrading enzyme and suppressed the activities of protein\uffe2\uff80\uff90 and chitin\uffe2\uff80\uff90degrading enzymes but had no effect on soil respiration rates or 14C signatures. These results indicate that N fertilization alters microbial community composition and allocation to extracellular enzyme production without affecting soil respiration. Thus, our results do not provide evidence for strong microbial feedbacks to the boreal C cycle under climate warming or N addition. However, organic N cycling may decline due to a reduction in the activity of enzymes that target nitrogenous compounds.

", "keywords": ["2. Zero hunger", "nucleotide analog", "Ecology", "microbial biomass", "ectomycorrhizal fungi", "extracellular enzyme", "nitrogen fertilization", "04 agricultural and veterinary sciences", "15. Life on land", "Biological Sciences", "soil respiration", "Environmental sciences", "Biological sciences", "Earth sciences", "13. Climate action", "carbon cycle", "0401 agriculture", " forestry", " and fisheries", "boreal forest", "bacteria", "Alaska", "Environmental Sciences"]}, "links": [{"href": "https://escholarship.org/content/qt5dg6p7gm/qt5dg6p7gm.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2008.01549.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2008.01549.x", "name": "item", "description": "10.1111/j.1365-2486.2008.01549.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2008.01549.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-01-20T00:00:00Z"}}, {"id": "10.5061/dryad.4qrfj6qg2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:22Z", "type": "Dataset", "created": "2023-07-07", "title": "Depth-dependent effects of Ericoid Mycorrhizal shrubs on soil carbon and nitrogen pools are accentuated under Arbuscular Mycorrhizal Trees", "description": "unspecifiedWe worked in a 3,213-ha second-growth, mixed-hardwood forest in Connecticut, USA (41\u00b057\u2019 N, 72\u00b007\u2019 W). We established 18 10-m radius plots, each containing a pair of 1-m radius subplots (n =36), evenly arrayed across three forest stands that contained areas of both high AM and high EcM tree relative basal area as well as a patchy distribution of the ErM shrub Kalmia latifolia.\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0 \u00a0 Within each of the 18 plots, we established paired 1-m radius subplots with and without K. latifolia in the understory ( \u201c+/- ErM subplot\u201d) within 2 m of the center of the 10-m radius plot. In each 1-m radius subplot, we measured trees \u22651 cm diameter at breast height (DBH; 1.37 m). We also measured DBH of all trees \u226520 cm DBH within 10 m and trees \u22655 cm DBH within 5 m of plot center. We calculated the percentage of EcM tree basal area out of total basal area, scaled to m2 ha-1. \u00a0 In June 2021, we collected and pooled two soil samples for each of three depths within the 36 paired subplots (i.e. 18 +ErM and 18 -ErM subplots). The three depths included: (1) the Oa horizon (depth varied depending on the thickness of the horizon); (2) the top 10 cm of the A horizon, beginning at the base of the Oa horizon; and (3) a second, contiguous A horizon sample that reached a cumulative sampling depth of 30 cm, inclusive of the depth of the Oa horizon. For the organic layer, we removed the litter layer (i.e. the Oi and Oe horizons) and collected and pooled two 25 by 25-cm areas of the Oa horizon using a square template. For the mineral layers, we collected two contiguous depth increments from the A horizon within the footprint of the 25 by 25-cm areas using a 5.08-cm diameter hammer corer. In each instance, we recorded the exact sampling depth. Two subplots did not have an Oa horizon, so we collected a total of 106 samples (3 sites \u00d7 6 plots \u00d7 2 subplots \u00d7 3 depths \u2212 2 Oa samples). Soils were stored at 4\u00b0C prior to their analysis. \u00a0 To prepare the soil samples for analysis, we weighed and homogenized each sample, air dried a representative subsample of non-sieved soil, and passed the remaining field-moist sample through a 4-mm sieve. Using the non-sieved subsample, we estimated the mass and volume of roots and stones and calculated soil bulk density values. For total soil organic matter (SOM) content, we heated samples at 550\u00b0C for 12-h in a muffle furnace and calculated loss on ignition. \u00a0 We used a modified substrate-induced respiration method as an indicator of active saprotrophic microbial biomass. Using autolyzed yeast extract solution as a labile C substrate, we measured rates of CO2 efflux over a 4-h incubation period with an Infra-Red Gas Analyzer and calculated the rate of C-CO2 production per unit of equivalent soil dry mass. For microbially-available C, we estimated potential CO2 production rates over a 14-d incubation period. We measured CO2 efflux over 24-h periods at days 1, 5, 8, and 14 and integrated the four measurements to calculate cumulative C-CO2 production. We estimated water holding capacity by saturating each field-moist sample with water and allowing it to drain freely for 2 h. To calculate the equivalent dry mass of field-moist samples, we measured gravimetric water content by oven-drying the samples to constant mass at 105\u00b0C. \u00a0 We separated the >53 and <53\u2009\u00b5m particle size fractions to quantify particulate (POM) and mineral-associated soil organic matter fractions. We passed air-dried samples through a 2-mm sieve and then dispersed soil aggregates by shaking ~30 g of the sieved, air-dried sample with 30\u2009mL of sodium hexametaphosphate (NaHMP) solution for 18 h. We rinsed each sample over a 53-\u00b5m sieve with deionized water until the water passing through the sieve ran clear. We oven-dried the >53-\u00b5m fraction retained on the top of the sieve and a representative subsample of the <53-\u00b5m fraction suspended in solution at 70\u00b0C. To estimate the mass of the <53-\u00b5m fraction, we calculated the difference between the initial soil mass (105\u00b0C equivalent) and the recovered mass of the >53-\u00b5m fraction (105\u00b0C equivalent). To convert air-dried soil mass to oven-dried mass we dried a subsample of each air-dried sample at 105\u00b0C. Fractions were ground to a fine powder and analyzed for total carbon (C) and nitrogen (N) concentrations using a Costech ESC 4010 Elemental Analyzer. \u00a0 We used an equivalent soil mass approach to calculate soil C, N, SOM, microbial biomass, and microbially-available C stocks in three equivalent soil mass layers as well as the sum of the three layers to estimate cumulative stocks at the subplot level. Following this approach, we report stocks to a standard soil mass and therefore allow the depth of the equivalent soil mass layers to vary depending on soil bulk density. To calculate equivalent soil mass stocks, we added or subtracted elemental stocks of the deeper soil layer to the upper soil layer in 1-mm increments until the soil mass from the upper layer is closest to that of the target soil mass. We chose reference soil masses using the median or target field sampling depth and the mean bulk density value for each of the three depth increments to make them roughly equivalent to the sampled depths. Based on this method, the organic layer had an equivalent mass of ~2.5 kg soil m-2 (median Oa depth = 2.5 cm; mean Oa bulk density = 0.10 g cm-3), the surface mineral layer had an equivalent mass of ~37 kg soil m-2 (target sampling depth = 10 cm; mean bulk density = 0.37 g cm-3), and the subsurface mineral layer had an equivalent mass of ~126 kg soil m-2 (the target sampling depth was 17.5 cm for a sample with a 2.5 cm Oa depth; mean bulk density = 0.72 g cm-3). The cumulative equivalent soil mass for the subplot-level stocks was the sum of the three layers, or ~166 kg soil m-2.", "keywords": ["equivalent soil mass", "ericoid mycorrhizal fungi", "13. Climate action", "ectomycorrhizal fungi", "Particulate organic matter", "FOS: Biological sciences", "soil nitrogen", "Arbuscular mycorrhizal fungi", "Mineral-associated organic matter", "soil carbon stocks", "15. Life on land"], "contacts": [{"organization": "Ward, Elisabeth", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.4qrfj6qg2"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.4qrfj6qg2", "name": "item", "description": "10.5061/dryad.4qrfj6qg2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.4qrfj6qg2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-07-12T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.00168", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:21:37Z", "type": "Journal Article", "created": "2019-02-26", "title": "Rapid Transfer of Plant Photosynthates to Soil Bacteria via Ectomycorrhizal Hyphae and Its Interaction With Nitrogen Availability", "description": "Plant roots release recent photosynthates into the rhizosphere, accelerating decomposition of organic matter by saprotrophic soil microbes ('rhizosphere priming effect') which consequently increases nutrient availability for plants. However, about 90% of all higher plant species are mycorrhizal, transferring a significant fraction of their photosynthates directly to their fungal partners. Whether mycorrhizal fungi pass on plant-derived carbon (C) to bacteria in root-distant soil areas, i.e., incite a 'hyphosphere priming effect,' is not known. Experimental evidence for C transfer from mycorrhizal hyphae to soil bacteria is limited, especially for ectomycorrhizal systems. As ectomycorrhizal fungi possess enzymatic capabilities to degrade organic matter themselves, it remains unclear whether they cooperate with soil bacteria by providing photosynthates, or compete for available nutrients. To investigate a possible C transfer from ectomycorrhizal hyphae to soil bacteria, and its response to changing nutrient availability, we planted young beech trees (Fagus sylvatica) into 'split-root' boxes, dividing their root systems into two disconnected soil compartments. Each of these compartments was separated from a litter compartment by a mesh penetrable for fungal hyphae, but not for roots. Plants were exposed to a 13C-CO2-labeled atmosphere, while 15N-labeled ammonium and amino acids were added to one side of the split-root system. We found a rapid transfer of recent photosynthates via ectomycorrhizal hyphae to bacteria in root-distant soil areas. Fungal and bacterial phospholipid fatty acid (PLFA) biomarkers were significantly enriched in hyphae-exclusive compartments 24 h after 13C-CO2-labeling. Isotope imaging with nanometer-scale secondary ion mass spectrometry (NanoSIMS) allowed for the first time in situ visualization of plant-derived C and N taken up by an extraradical fungal hypha, and in microbial cells thriving on hyphal surfaces. When N was added to the litter compartments, bacterial biomass, and the amount of incorporated 13C strongly declined. Interestingly, this effect was also observed in adjacent soil compartments where added N was only available for bacteria through hyphal transport, indicating that ectomycorrhizal fungi were acting on soil bacteria. Together, our results demonstrate that (i) ectomycorrhizal hyphae rapidly transfer plant-derived C to bacterial communities in root-distant areas, and (ii) this transfer promptly responds to changing soil nutrient conditions.", "keywords": ["Hyphosphere priming", "DYNAMICS", "0301 basic medicine", "PLFAs", "Microbiology", "ectomycorrhiza", "03 medical and health sciences", "Mycorrhizosphere", "MICROBIAL COMMUNITY COMPOSITION", "NanoSIMS", "hyphal carbon transfer", "hyphosphere bacteria", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "IDENTIFICATION", "RHIZOSPHERE", "15. Life on land", "QR1-502", "EXTRACTION METHOD", "Ectomycorrhiza", "ORGANIC-MATTER", "MYCORRHIZAL FUNGI", "hyphosphere priming", "mycorrhizosphere", "Hyphal carbon transfer", "106022 Microbiology", "FATTY-ACIDS", "Hyphosphere bacteria", "BAYESIAN CLASSIFIER", "CARBON ALLOCATION"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.00168"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.00168", "name": "item", "description": "10.3389/fmicb.2019.00168", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.00168"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-26T00:00:00Z"}}, {"id": "10.3389/fpls.2021.682142", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:21:40Z", "type": "Journal Article", "created": "2021-07-21", "title": "Shifts in the Abundances of Saprotrophic and Ectomycorrhizal Fungi With Altered Leaf Litter Inputs", "description": "

Ectomycorrhizal (EcM) and saprotrophic fungi interact in the breakdown of organic matter, but the mechanisms underlying the EcM role on organic matter decomposition are not totally clear. We hypothesized that the ecological relations between EcM and saprotroph fungi are modulated by resources availability and accessibility, determining decomposition rates. We manipulated the amount of leaf litter inputs (No-Litter, Control Litter, Doubled Litter) on Trenched (root exclusion) and Non-Trenched plots (with roots) in a temperate deciduous forest of EcM-associated trees. Resultant shifts in soil fungal communities were determined by phospholipid fatty acids and DNA sequencing after 3 years, and CO2 fluxes were measured throughout this period. Different levels of leaf litter inputs generated a gradient of organic substrate availability and accessibility, altering the composition and ecological relations between EcM and saprotroph fungal communities. EcM fungi dominated at low levels of fresh organic substrates and lower organic matter quality, where short-distances exploration types seem to be better competitors, whereas saprotrophs and longer exploration types of EcM fungi tended to dominate at high levels of leaf litter inputs, where labile organic substrates were easily accessible. We were, however, not able to detect unequivocal signs of competition between these fungal groups for common resources. These results point to the relevance of substrate quality and availability as key factors determining the role of EcM and saprotroph fungi on litter and soil organic matter decay and represent a path forward on the capacity of organic matter decomposition of different exploration types of EcM fungi.Study area The study was conducted in the Abitibi-T\u00e9miscamingue region, Quebec (Canada). The study area is located in northern Quebec\u2019s clay plain, within the balsam fir-paper birch (Abies balsamea (Linnaeus) Miller-Betula papyriferaMarshall) bioclimatic domain (Grondin 1996). Climatic conditions are subpolar, subhumid, and continental with an annual average temperature and precipitation of respectively between 1-2 \u00b0C and 825-975 mm according to the Canadian climate normal 1981-2010 station data (Environment Canada, 2023). Forest vegetation mainly consisted of jack pine stands with interspersed stems of Picea mariana (Miller) Britton, Sterns & Poggenburgh, Populus tremuloides Michaux, Betula papyrifera Marshall, Abies balsamea (Linnaeus) Miller, and Acer rubrum Linnaeus. Specifically, the Abitibi region is characterized by natural gradients of soil physicochemical properties, from sandy eskers to clayey soil types. Indeed, esker soils account for ~3% of the Abitibi\u2019s surface while the remainder of the region\u2019s flat topography (~57%) is dominated by clayey soils (Robitaille and Saucier 1998; Cloutier et al. 2007). Abitibi eskers have mineral surface deposits with an overall thickness of more than 25 cm, an illuvial horizon with coarse texture, and a high stone content. Conversely, glacio-lacustrine clayey soils, which are associated with Luvisols or Gleysols in the Abitibi region (Laverdi\u00e8re and De Kimpe 1984; Bergeron et al. 2007), are characterized by an illuvial horizon with medium texture and a low stone content (Blouin and Berger 2002). Site selection Site selection was based on four main criteria, namely soil type (sandy esker/clayey), dominant vegetation (jack pine, minimum 50-75% of canopy cover), stand age (minimum 50-60 years), stand origin (natural fire-origin, totally or mostly unimpacted by anthropogenic disturbance), and accessibility (maximum 350 m from forest roads). We used satellite images and forest inventory data from the Quebec government\u2019s online platform \u2018For\u00eat Ouverte\u2019 for site selection. We selected 18 natural jack pine stands (12 on sandy eskers, 6 on the clay plain). Only six clayey sites fitting our selection criteria were found within the prospected territory due to the scarcity of unmanaged (natural) jack pine stands on clayey soils. Sites were separated by a minimum distance of 5 km to ensure independence between samples and avoid pseudoreplication. Finally, the study design consisted of a first-level factor (soil type) with two levels (sand and clay), and a second-level factor (soil horizons) with three levels (litter, organic, and mineral). Soil sampling Soil samples were collected during the summer of 2022 to characterize soil fungal communities and obtain the physicochemical composition across the soil profile. A circular plot with a 28 m radius and an area of 2,500 m2 was established at each study site, at least 20 m from the forest edge to avoid related variability bias (Dickie and Reich 2005). Within each plot, five jack pine trees were randomly selected (at least 10 m apart), and two soil sample replicates per tree (2-3 m apart) were collected, following a modified protocol by Tedersoo et al. (2014; 2021). Sampling locations around trees were randomly selected with the restriction criteria of being strictly opposite (angle of 180\u00b0). In total, 180 soil cores (2 replicates \u00d7 5 trees \u00d7 18 sites) were collected. For each sampling spot, forest fallen litter (dead leaves, needles) (hereafter referred to as litter horizon) was collected in plastic bags for DNA and physicochemical analyses, followed by samples from the organic layer (hereafter referred to as organic horizon). The latter represented a dark-colored layer rich in organic matter at various decomposition stages, mainly composed of fallen plant material. These organic samples included both F and H horizons. After the removal of the organic layer, we sampled the mineral soil (hereafter referred to as mineral horizon) using a pedological auger (25 cm in length and 7.5 cm in diameter), capturing both eluvial and illuvial horizons. For esker sites, the mineral soil profile was characterized by the formation and accumulation of organo-metallic assemblages, involving Al and Fe elements, in a leached grey eluvial and rust-brown illuvial horizon, respectively. Due to the variable thickness of the eluvial horizons within both esker and clayey soil profiles, the proportion of this horizon in the mineral soil samples varied among sites. Large roots and coarse woody debris were systematically removed from organic and mineral material while sampling. Samples were pooled per horizon for each site, resulting in one composite sample for each horizon. This represents 18 composite samples per horizon for a total of 54 composite soil samples (3 horizons \u00d7 18 sites). Each composite soil samples were divided into two sub-samples: one for eDNA-based soil fungal community analysis and the other for physicochemical analysis. Composite organic and mineral soil samples for eDNA analysis were sieved with a 6 mm mesh in the field, placed in plastic bags, transported in an ice-filled cooler and stored at \u2013 25\u00b0C in the Ecology Research Group of Abitibi RCM (GREMA) laboratory until further processing. Composite litter samples for eDNA analysis were crushed in liquid nitrogen using a mortar and pestle prior to DNA extraction. Composite soil samples for physicochemical analyses were sieved with a 4 mm mesh using an automatic vibrating sieve AS 200 Control (ATS Care Retsch, Haan, Germany) after being forced-air dried at room temperature for 14 days to facilitate the sieving process. Soil physicochemical analyses Composite soil samples (> 50g) were sent to the organic and inorganic chemistry laboratory of the Forest Research Direction (Quebec, QC, Canada) for physicochemical analysis. Total nitrogen (N) and carbon (C) contents were determined by combustion using a CN 928 elemental analyzer (LECO Corp., St Joseph, MI, USA) with thermal conductivity detection for nitrogen and non-dispersive infra-red (NDIR) cell detection for carbon. Organic matter content (hereafter OM) and percentage of humidity (hereafter humidity) were determined by incineration, a method commonly referred to as the loss on ignition (LOI) method (Davies 1974). Soil pH was measured using 10 g of soil mixed with 20 mL of distilled water with an Orion VersaStar Pro pH meter (Thermo Fisher Scientific Inc., Pittsburgh, PA, USA). Elements (P, K, Ca, Mg, Mn, Zn, Al, Fe) were extracted using the Mehlich-III method (Mehlich 1984) and measured by plasma atomic emission spectroscopy (Optima 8300 model, ICP-OES, Perkin Elmer, Waltham, MA, USA). Particle size distribution and textural class determination were performed on soil samples consisting of fine earth (<2 mm) containing 5% or less carbon using the Bouyoucos method (Bouyoucos 1962). DNA extraction, amplification and library preparation DNA extraction was carried out at the Environmental Genomic Laboratory of the Laurentian Forestry Centre (EGL-CFL) on 150 mg of composite litter and organic samples, and 250 mg of composite mineral samples using a DNeasy powersoil pro kit (Qiagen, Valencia, CA, US) and the QIAcube automated instrument (Qiagen, Hilden, Germany) in accordance with the manufacturer\u2019s instructions. DNA was quantified with the Qubit\u2122 dsDNA BR Assay Kit (Thermo Fisher Scientific Inc, Wilmington, USA). Fungal DNA amplicon libraries were prepared at the EGL-CFL following a previously described procedure (Samad et al. 2023) and sequenced on an Illumina MiSeq platform at the Next Generation Sequencing Platform of the CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre using a MiSeq v3 600-cycle Reagent Kit. Even though one ITS region contains less genetic information than the entire ITS (Tedersoo et al. 2022), the ITS2 region of the fungal ribosomal DNA was amplified using the primer set ITS9F (5\u2019-GAACGCAGCRAAIIGYGA-3\u2019) and ITS4R (5\u2019-TCCTCCGCTTATTGATATGC-3\u2019 (White et al. 1990; Ihrmark et al. 2012; Rivers 2016) because of limitations to sequence length that can be obtained with Illumina Sequencing. Negative controls in the DNA extraction and PCR amplification were used to control potential contaminants during the process. Bioinformatic and sequence analyses All bioinformatics analyses were performed in QIIME2 v2023.2.0 (Bolyen et al. 2019). Demultiplexed FASTQ (R1 and R2) files were first imported in QIIME2 using the \u2018qiime import\u2019 command which converts the data into a QZA archive file so it can be processed by the rest of the QIIME2 workflow. First, primers were removed using the QIIME2 implementation of CutAdapt (Martin 2011). Resulting forward and reverse reads went through the DADA2 (Callahan et al. 2016) pipeline for sequence quality control and feature table construction using the \u2018\u2019qiime dada2 denoise-paired\u2019\u2019 command. During this process, low-quality regions of the sequences were trimmed, paired reads assembled, chimeric sequences filtered, remaining high-quality sequences dereplicated, and singletons and very low-frequency abundance ASVs removed using the \u201cqiime feature-table filter-features\u201d command. The output of this step was a feature table (i.e., ASV table) which contains read counts for each unique sequence in each sample of the dataset, and feature data which contains sequences corresponding to each ASV. A taxonomy was assigned to each ASV based on the UNITE reference database (version 9.0) (Abarenkov et al. 2010, 2023; K\u00f5ljalg et al. 2005, 2013; Nilsson et al. 2019b) using the \u2018\u2018classify-sklearn\u2019\u2019 with a Naive Bayes classifier. All ASVs not assigned to the \u2018Fungi\u2019 kingdom were removed from further analyses using the \u201cqiime taxa filter-table\u201d. The resulting fungal ASV table was used to perform downstream statistical analyses. Functional annotation of ASVs was performed for most fungal guilds using FUNGuild version 1.1 (Nguyen et al. 2016).", "keywords": ["Ectomycorrhiza", "eDNA metabarcoding", "Clay belt", "Pinus banksiana", "FOS: Biological sciences", "esker ecosystems", "saprotrophs"], "contacts": [{"organization": "Cazabonne, Jonathan, DesRochers, Annie, Martineau, Christine, Roy, M\u00e9lanie, Girona, Miguel Montoro,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.ffbg79d33"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.ffbg79d33", "name": "item", "description": "10.5061/dryad.ffbg79d33", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.ffbg79d33"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-13T00:00:00Z"}}, {"id": "10.5061/dryad.79cnp5htw", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:24Z", "type": "Dataset", "title": "Data from: A tipping-point in carbon storage when forest expands into tundra is related to mycorrhizal recycling of nitrogen", "description": "unspecifiedTundra ecosystems are global belowground sinks for atmospheric CO2. Ongoing warming-induced encroachment by shrubs and trees risks turning this sink into a CO2 source, resulting in a positive feedback on climate warming. To advance mechanistic understanding of how shifts in mycorrhizal types affect long-term carbon (C) and nitrogen (N) stocks, we studied small-scale soil depth profiles of fungal communities and C-N dynamics across a subarctic-alpine forest-heath vegetation gradient. Belowground organic stocks decreased abruptly at the transition from heath to forest, linked to the presence of certain tree-associateds ectomycorrhizal fungi that contribute to decomposition when mining N from organic matter. In contrast, ericoid mycorrhizal plants and fungi were associated with organic matter accumulation and slow decomposition. If climatic controls on arctic-alpine forest lines are relaxed, increased decomposition will likely outbalance increased plant productivity, decreasing the overall C sink capacity of displaced tundra.", "keywords": ["C-N dynamics", "ectomycorrhizal exploration type", "functional genes", "ergosterol", "ITS2 meta-barcoding", "Fungal community", "Arctic greening", "Climate feedback", "15. Life on land", "litter saprotrophs", "mycorrhizal type", "litter bags", "13. Climate action", "soil solution", "FOS: Biological sciences", "soil carbon storage", "quantitative PCR", "soil profiles", "Ectomycorrhizal fungal community", "Ericoid Mycorrhiza", "treeline ecotone"], "contacts": [{"organization": "Clemmensen, Karina E, Durling, Mikael B, Michelsen, Anders, Hallin, Sara, Finlay, Roger D, Lindahl, Bj\u00f6rn D,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.79cnp5htw"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.79cnp5htw", "name": "item", "description": "10.5061/dryad.79cnp5htw", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.79cnp5htw"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-28T00:00:00Z"}}, {"id": "10.5061/dryad.3xsj3txkf", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:22Z", "type": "Dataset", "title": "The diversity of mycorrhiza-associated fungi and trees shape subtropical mountain forest ecosystem functioning", "description": "Aim: Mycorrhiza play key roles in ecosystem structure and functioning in forests. However, how different mycorrhizal types influence mountain forest biodiversity-ecosystem functioning relationships is largely unknown. We evaluate how the diversity of distinct mycorrhiza-associated fungi and trees shape forest carbon storage along elevational gradients. Location: Gaoligong Mountains within Hengduan Mountains, Southwest China. Taxon: Seed plants and mycorrhizal fungi. Methods: We used the data from 31 subtropical forest plots along elevational gradients on two aspects (east and west) of the mountain. We quantified species richness of trees and symbiotic fungi and assigned both to their mycorrhizal type (arbuscular mycorrhiza (AM), ectomycorrhiza (EcM) and ericoid mycorrhiza (ErM)). We then examined the diversity effects of mycorrhiza-associated fungi and trees on above-ground carbon stored in trees and organic carbon stored in soils. Results: Species richness was highest for AM trees (79.5%), followed by ErM trees (13.4%) and then EcM trees (7.1%). Species richness of AM-associated trees and fungi decreased with increasing elevation, while ErM-associated trees and fungi showed an opposite trend. EcM-associated diversity followed a hump-shaped relationship with elevation. Positive relationships between diversity and above-ground carbon were detected in all three mycorrhizal associations, but despite low species number, canopy-dominating EcM trees comprised 64.4% of the amount of above-ground carbon. Furthermore, community-weighted means of height exhibited positive correlations with forest above-ground carbon, indicating that positive selection effects occur. Soil organic carbon was positively related to EcM-associated fungi diversity, above-ground carbon mass and soil nitrogen availability, with the latter having the strongest direct effects. Main conclusions: The distributions of forest biodiversity and carbon storage can be modulated by distinct mycorrhizal fungi and trees. Moreover, future global changes (e.g., climate warming, intensifying nitrogen deposition) could alter the mycorrhizal-mediated biodiversity-ecosystem functioning relationships in mountain forests.", "keywords": ["Ectomycorrhiza", "soil organic carbon", "13. Climate action", "arbuscular mycorrhiza", "FOS: Biological sciences", "elevational gradients", "14. Life underwater", "15. Life on land", "above-ground carbon", "functional diversity"], "contacts": [{"organization": "Luo, Ya-Huang, Ma, Liang-Liang, Seibold, Sebastian, Cadotte, Marc W., Burgess, Kevin, Tan, Shao-Lin, Ye, Lin-Jiang, Zheng, Wei, Zou, Jia-Yun, Chen, Zhi-Fa, Liu, De-Tuan, Zhu, Guang-Fu, Shi, Xiao-Chun, Zhao, Wei, Li, De-Zhu, Liu, Jie, Gao, Lian-Ming,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.3xsj3txkf"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.3xsj3txkf", "name": "item", "description": "10.5061/dryad.3xsj3txkf", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.3xsj3txkf"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-20T00:00:00Z"}}, {"id": "10.5061/dryad.4b8gthtcn", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:22Z", "type": "Dataset", "title": "Ericoid mycorrhizal shrubs alter the relationship between tree mycorrhizal dominance and soil carbon and nitrogen", "description": "unspecifiedThis dataset is comprised of three tabs in a single excel file. See the 'metadata' tab for information pertaining to the variables measured and analyzed. The 'CT_data' tab includes values for all the soil variables analyzed and reported on from the Connecticut site. The 'USNPS_data' tab includes the percent vegetation cover for each plant taxon by stratum/vegetation layer for each of the U.S. National Park Service plots analyzed and reported on in the manuscript.", "keywords": ["ectomycorrhizal fungi", "soil organic matter", "fungal interactions", "Forest understorey", "Arbuscular mycorrhizal fungi", "15. Life on land"], "contacts": [{"organization": "Ward, Elisabeth, Duguid, Marlyse, Kuebbing, Sara, Lendemer, James, Warren II, Robert, Bradford, Mark,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.4b8gthtcn"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.4b8gthtcn", "name": "item", "description": "10.5061/dryad.4b8gthtcn", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.4b8gthtcn"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-28T00:00:00Z"}}, {"id": "10.5061/dryad.0m9n57k", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:20Z", "type": "Dataset", "title": "Data from: The effect of drought and season on root life span in temperate arbuscular mycorrhizal and ectomycorrhizal tree species", "description": "unspecifiedLiese_etal_morphological_and_functional_traitsBelowground and aboveground morphological and functional traitsLiese_etal_lifespan_and_proportional_hazardsRoot lifespan and data for the calculation of proportional hazards", "keywords": ["2. Zero hunger", "Ectomycorrhiza", "plant-soil (below-ground) interactions", "mini-rhizotrons", "deciduous tree species", "arbuscular mycorrhiza", "Season", "15. Life on land", "root morphology", "6. Clean water"], "contacts": [{"organization": "Liese, Rebecca, Leuschner, Christoph, Meier, Ina Christin,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.0m9n57k"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.0m9n57k", "name": "item", "description": "10.5061/dryad.0m9n57k", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.0m9n57k"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-10T00:00:00Z"}}, {"id": "10.5061/dryad.845kg37", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:24Z", "type": "Dataset", "title": "Data from: Resource acquisition strategies facilitate Gilbertiodendron dewevrei monodominance in African lowland forests", "description": "unspecified1. Tropical forests are hyperdiverse, yet extensive areas of monodominant forest occur in the tropics worldwide. Most long-lived and persistent monodominant tree species form ectomycorrhizal fungi symbioses, allowing them to obtain nutrients directly from soil organic matter. This might promote monodominance by reducing nutrient availability to co-occurring species, the majority of which form associations with arbuscular mycorrhizal fungi. 2. Gilbertiodendron dewevrei forest is the most widespread monodominant forest in tropical Africa. Its distribution appears determined in part by moisture availability, but its monodominance is not thought to be driven by its fungal partner or soil fertility. 3. Here we compare soil fertility of twenty G. dewevrei stands to mixed forest from three sites across an 8,400 km2 region of the Central African Republic and the Republic of Congo. In contrast to previous studies, we find monodominant G. dewevrei stands associated with infertile soils, as base cations (calcium, magnesium, total exchangeable bases) and extractable manganese are extremely low, and significantly lower in soils under G. dewevrei forest compared to mixed forest. Further, and consistent with ectomycorrhizal forests globally, soil carbon to nitrogen and carbon to phosphorus ratios are significantly higher in G. dewevrei stands than in mixed forest stands, providing evidence in support of direct acquisition of nitrogen and phosphorus from soil organic matter by ectomycorrhizal fungi. 4. Gilbertiodendron dewevrei recruits from the seedling bank, with its large seedlings surviving in high densities for over a decade. We tested whether light plasticity could facilitate monodominance by growing seedlings of G. dewevrei under controlled light conditions. We found that its seedlings grow well under a wide range of irradiance levels and conclude that this plasticity affords a competitive advantage. 5. Synthesis: We reframe the discussion of factors contributing to monodominance of G. dewevrei into one of resource acquisition and use efficiency. In particular, G. dewevrei is associated with moist and infertile soils and competes well under a variety of light conditions. Our data is consistent with a model where root associations with ectomycorrhizal fungi drive monodominance through the direct acquisition of nutrients from soil organic matter, promoting nutrient limitation of co-occurring species.", "keywords": ["2. Zero hunger", "C:N and C:P ratios", "Congo Basin", "Central Africa", "ectomycorrhizal fungi", "15. Life on land", "Monodominant tropical forest", "Gilbertiodendron dewevrei"], "contacts": [{"organization": "Hall, Jefferson, Harris, David, Saltonsall, Kristin, Medjibe, Vincent, Ashton, Mark, Turner, Benjamin,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.845kg37"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.845kg37", "name": "item", "description": "10.5061/dryad.845kg37", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.845kg37"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-26T00:00:00Z"}}, {"id": "10.5061/dryad.ht4t3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:28Z", "type": "Dataset", "title": "Data from: Host phenology and potential saprotrophism of ectomycorrhizal fungi in the boreal forest", "description": "Open AccessPhenology-induced changes in carbon assimilation by trees may affect carbon stored in fine roots and as a consequence, alter carbon allocated to ectomycorrhizal fungi. Two competing models exist to explain carbon mobilization by ectomycorrhizal fungi. Under the \u2018saprotrophy model\u2019, decreased allocation of carbon may induce saprotrophic behaviour in ectomycorrhizal fungi, resulting in the decomposition of organic matter to mobilize carbon. Alternatively, under the \u2018nutrient acquisition model\u2019, decomposition may instead be driven by the acquisition of nutrients locked within soil organic matter compounds, with carbon mobilization a secondary process. We tested whether phenology-induced shifts in carbon reserves of fine roots of aspen (Populus tremuloides) affect potential activity of four carbon-compound degrading enzymes, \u03b2-glucuronidase, \u03b2-glucosidase, N-acetylglucosaminidase and laccase, by ectomycorrhizal fungi. Ectomycorrhizal roots from mature aspen were collected across eight stands in north-eastern Alberta, Canada, and analysed during tree dormancy, leaf flush, full leaf expansion and leaf abscission. We predicted potential extracellular enzyme activity to be highest when root carbon reserves were lowest, should host phenology induce saprotrophism. Further, we anticipated enzyme activity to be mediated by invertase, a plant-derived enzyme which makes carbon available to fungal symbionts in the plant\u2013fungus interface. Root carbon reserves were positively correlated with invertase, suggesting phenology may affect carbon allocation to ectomycorrhizal fungi. However, of the four enzymes, host phenology had the largest effect on \u03b2-glucuronidase, but activity of this enzyme was not correlated with root carbon reserves or invertase. Low-biomass ectomycorrhizas had greater potential laccase activity than high-biomass ectomycorrhizas, highlighting discrete functional traits in fungi for litter decomposition. Our results suggest that the decomposition of organic matter may be driven by foraging by fungi for nutrients locked within organic compounds rather than for mobilizing carbon. Furthermore, the potential ability to degrade lignin was more common in low-biomass ectomycorrhizas when compared to high-biomass ectomycorrhizas.", "keywords": ["2. Zero hunger", "ectomycorrhizal exploration type", "extracellular enzymes", "15. Life on land", "nonstructural carbohydrates", "invertase"], "contacts": [{"organization": "Hupperts, Stefan F., Pritsch, Karin, Landh\u00e4usser, Simon M., Karst, Justine,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.ht4t3"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.ht4t3", "name": "item", "description": "10.5061/dryad.ht4t3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.ht4t3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-06-06T00:00:00Z"}}, {"id": "10.5061/dryad.jwstqjqc0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:29Z", "type": "Dataset", "title": "More soil organic carbon is sequestered through the mycelium-pathway than through the root-pathway under nitrogen enrichment in an alpine forest", "description": "Open AccessPeer reviewed", "keywords": ["roots", "SOC sequestration", "ectomycorrhizal mycelia", "Alpine forests", "15. Life on land", "Roots", "alpine forests", "6. Clean water", "N deposition", "Ectomycorrhizal mycelia", "Natural sciences", "microbial C pump", "Microbial C pump", "FOS: Natural sciences"], "contacts": [{"organization": "Zhu, Xiaomin, Zhang, Ziliang, Wang, Qitong, Pe\u00f1uelas, Josep, Sardans, Jordi, Li, Na, Liu, Qing, Yin, Huajun, Liu, Zhanfeng, Lambers, Hans,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.jwstqjqc0"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.jwstqjqc0", "name": "item", "description": "10.5061/dryad.jwstqjqc0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.jwstqjqc0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10.5061/dryad.rbnzs7hhb", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:31Z", "type": "Dataset", "created": "2023-09-28", "title": "Carbon availability affects already large species-specific differences in chemical composition of ectomycorrhizal fungal mycelia in pure culture", "description": "unspecifiedAlthough ectomycorrhizal (ECM) contribution to soil organic matter processes receives increased attention, little is known about fundamental differences in chemical composition among species, and how that may be affected by carbon (C) availability. Here we study how 16 species (incl. 19 isolates) grown in pure culture at three different C:N ratios (10:1, 20:1 and 40:1) vary in chemical structure, using Fourier transform infrared (FTIR) spectroscopy. We hypothesised that C availability impacts directly on chemical composition, expecting increased C availability to lead to more carbohydrates and less proteins in the mycelia. There were strong and significant effects of ECM species (R2 = 0.873 and P = 0.001) and large species-specific differences in chemical composition. Chemical composition also changed significantly with C availability, and increased C led to more polysaccharides and less proteins for many species, but not all. Understanding how chemical composition change with altered C availability is a first step towards understanding their role in organic matter accumulation and decomposition.", "keywords": ["Pure culture", "cell wall composition", "carbon availability", "ectomycorrhizal fungi", "Carbohydrates", "Fungi", "Chemical composition", "Fourier-transform infrared spectroscopy", "Proteins", "15. Life on land", "C:N ratio", "soil organic carbon", "FTIR spectra", "FOS: Biological sciences", "mycelia"], "contacts": [{"organization": "Fransson, Petra, Robertson, A H Jean, Campbell, Colin D,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.rbnzs7hhb"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.rbnzs7hhb", "name": "item", "description": "10.5061/dryad.rbnzs7hhb", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.rbnzs7hhb"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-05T00:00:00Z"}}, {"id": "10.5061/dryad.s4mw6m97j", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:31Z", "type": "Dataset", "title": "Links between boreal forest management, soil fungal communities and belowground carbon sequestration", "description": "Forest management has a potential to alter belowground carbon storage. However, the underlying mechanisms, and the relative importance of carbon input and decomposition in regulation of soil carbon dynamics are poorly understood. We examined whether interactive effects of forest fertilization and thinning on carbon stocks in the topsoil of boreal forests were linked to changes in fungal community composition, biomass, and enzyme activities, in a long-term fertilization and thinning experiment distributed across 29 Pinus sylvestris forests along a 1300 km latitudinal transect in Sweden. Nitrogen fertilization increased fungal biomass, particularly towards the north and mainly by promoting root associated Ascomycetes, but the response was moderated by thinning. Fungal biomass correlated positively with carbon stocks in the organic topsoil. However, ectomycorrhizal Cortinarius species were reduced in abundance by fertilization and correlated negatively with carbon stocks. Plausibly, increased soil carbon stocks after fertilization are linked to increased input of carbon in the form of root-associated mycelium combined with loss of ectomycorrhizal decomposers within the genus Cortinarius. These fungal responses to fertilization may mediate a natural climate solution by promoting carbon sequestration in the organic topsoil, but the effect of fertilization may also be undesired from a biodiversity perspective.", "keywords": ["Ectomycorrhiza", "13. Climate action", "Nitrogen", "Fungal community", "thinning", "fungal biomass", "15. Life on land", "Carbon"], "contacts": [{"organization": "J\u00f6rgensen, Karolina, Granath, Gustaf, Strengbom, Joachim, Lindahl, Bj\u00f6rn,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.s4mw6m97j"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.s4mw6m97j", "name": "item", "description": "10.5061/dryad.s4mw6m97j", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.s4mw6m97j"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-22T00:00:00Z"}}, {"id": "10451/59994", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:25:58Z", "type": "Journal Article", "created": "2022-10-27", "title": "Sustainable production of ectomycorrhizal fungi in the Mediterranean region to support the European Green Deal", "description": "Societal Impact Statement

The planet faces a climate crisis with severe health, economic and environmental consequences. Political actions such as the European Green Deal aim to mitigate climate change by shifting production and consumption patterns, and the production of mycorrhizal sporocarps\uffe2\uff80\uff94the fruiting body of fungi\uffe2\uff80\uff94is no exception. The production of mycorrhizal sporocarps has a high economic, cultural and environmental impact in the Mediterranean region. With a key role in forest ecosystems, ectomycorrhizal fungi provide services and goods essential to maintain soil quality, ecosystem functions and food, contributing to the achievement of sustainable production and the European Green Deal goals\uffe2\uff80\uff94a climate\uffe2\uff80\uff90neutral Europe.

Summary

Ectomycorrhizal fungi (ECMF) cultivation is an important economic activity in the Mediterranean region. Sporocarps from ECMF species such as Terfezia claveryi, Tuber melanosporum, Tuber aestivum and Lactarius delicious have been successfully cultivated. Due to biotechnological advances, a considerable evolution in ECMF cultivation techniques was observed in the last decade. New technologies and intensified Research and Development allow for a better understanding of the physiology of the plant\uffe2\uff80\uff90fungi symbioses and how climate change affects them. Studying forest management practices is also essential to optimise the natural production of ectomycorrhizal sporocarps and help develop sustainable production practices. This knowledge revealed the importance of ECMF and their role in the rural bioeconomy and highlighted the need to establish sustainable cultivation practices. A successful example of ECMF cultivation is the production of Terfezia species, namely, Terfezia claveryi and Terfezia boudieri. Terfezia truffles are traditional delicacies with high socioeconomic relevance and numerous biotechnological applications. Furthermore, these Mediterranean native species are an important tool to develop the bioeconomy in rural areas by creating new production strategies. Furthermore, exploiting these and other native Mediterranean species can promote sustainable practices in line with new European Green Deal strategies, such as the Farm to Fork strategy, the EU Biodiversity strategy for 2030 and the Climate Law. This work reviews ECMF cultivation practices and forest management studies, presenting the case of Terfezia cultivation and how the sustainable production of wild and planted ECMF may contribute to achieving the European Green Deal objectives and to a more resilient Europe.Ectomycorrhizal (EcM) and saprotrophic fungi interact in the breakdown of organic matter, but the mechanisms underlying the EcM role on organic matter decomposition are not totally clear. We hypothesized that the ecological relations between EcM and saprotroph fungi are modulated by resources availability and accessibility, determining decomposition rates. We manipulated the amount of leaf litter inputs (No-Litter, Control Litter, Doubled Litter) on Trenched (root exclusion) and Non-Trenched plots (with roots) in a temperate deciduous forest of EcM-associated trees. Resultant shifts in soil fungal communities were determined by phospholipid fatty acids and DNA sequencing after 3 years, and CO2 fluxes were measured throughout this period. Different levels of leaf litter inputs generated a gradient of organic substrate availability and accessibility, altering the composition and ecological relations between EcM and saprotroph fungal communities. EcM fungi dominated at low levels of fresh organic substrates and lower organic matter quality, where short-distances exploration types seem to be better competitors, whereas saprotrophs and longer exploration types of EcM fungi tended to dominate at high levels of leaf litter inputs, where labile organic substrates were easily accessible. We were, however, not able to detect unequivocal signs of competition between these fungal groups for common resources. These results point to the relevance of substrate quality and availability as key factors determining the role of EcM and saprotroph fungi on litter and soil organic matter decay and represent a path forward on the capacity of organic matter decomposition of different exploration types of EcM fungi.

It is known that ectomycorrhizal (ECM) fungi can modulate below and aboveground communities. They are a key part of belowground communication as they produce a vast array of metabolites, including volatile organic compounds (VOCs) such as 1-octen-3-ol. Here, we tested if the VOC 1-octen-3-ol may be involved in the ECM fungal mechanisms that modulate below and aboveground communities. For that, we conducted three in vitro assays with ECM fungi and the 1-octen-3-ol volatile to (i) explore the effects of mycelium growth of three ECM species, (ii) investigate the impact on the germination of six host Cistaceae species, and (iii) study the impact on host plant traits. The effects of 1-octen-3-ol on mycelium growth of the three ECM species depended on the dose and species: Boletus reticulatus was the most sensitive species to the low (VOC) dose, while T. leptoderma was the most tolerant. In general, the presence of the ECM fungi resulted in higher seed germination, while 1-octen-3-ol resulted in lower seed germination. The combined application of the ECM fungus and the volatile further inhibited seed germination, possibly due to the accumulation of 1-octen-3-ol above the plant species\u2019 threshold. Seed germination and plant development of Cistaceae species were influenced by ECM fungal volatiles, suggesting that 1-octen-3-ol may mediate changes in below and aboveground communities.

", "keywords": ["0301 basic medicine", "0303 health sciences", "ectomycorrhizal fungi", "QH301-705.5", "Cistaceae", "15. Life on land", "Article", "fungal volatiles", "03 medical and health sciences", "1-octen-3-ol", "Biology (General)", "C-8 volatiles", "Terfezia", "1-octen-3-ol; C-8 volatiles; fungal volatiles; ectomycorrhizal fungi; Terfezia; Cistaceae"]}, "links": [{"href": "http://www.mdpi.com/2309-608X/9/2/180/pdf"}, {"href": "https://repositorio.ulisboa.pt/bitstream/10451/58419/1/jof-09-00180.pdf"}, {"href": "https://repositorio.ulisboa.pt/bitstream/10451/60000/1/Ferreira%20et%20al%202023.pdf"}, {"href": "https://www.mdpi.com/2309-608X/9/2/180/pdf"}, {"href": "https://doi.org/10451/60000"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Fungi", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10451/60000", "name": "item", "description": "10451/60000", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10451/60000"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-29T00:00:00Z"}}, {"id": "11353/10.1146400", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:26:08Z", "type": "Journal Article", "created": "2019-02-26", "title": "Rapid Transfer of Plant Photosynthates to Soil Bacteria via Ectomycorrhizal Hyphae and Its Interaction With Nitrogen Availability", "description": "Plant roots release recent photosynthates into the rhizosphere, accelerating decomposition of organic matter by saprotrophic soil microbes ('rhizosphere priming effect') which consequently increases nutrient availability for plants. However, about 90% of all higher plant species are mycorrhizal, transferring a significant fraction of their photosynthates directly to their fungal partners. Whether mycorrhizal fungi pass on plant-derived carbon (C) to bacteria in root-distant soil areas, i.e., incite a 'hyphosphere priming effect,' is not known. Experimental evidence for C transfer from mycorrhizal hyphae to soil bacteria is limited, especially for ectomycorrhizal systems. As ectomycorrhizal fungi possess enzymatic capabilities to degrade organic matter themselves, it remains unclear whether they cooperate with soil bacteria by providing photosynthates, or compete for available nutrients. To investigate a possible C transfer from ectomycorrhizal hyphae to soil bacteria, and its response to changing nutrient availability, we planted young beech trees (Fagus sylvatica) into 'split-root' boxes, dividing their root systems into two disconnected soil compartments. Each of these compartments was separated from a litter compartment by a mesh penetrable for fungal hyphae, but not for roots. Plants were exposed to a 13C-CO2-labeled atmosphere, while 15N-labeled ammonium and amino acids were added to one side of the split-root system. We found a rapid transfer of recent photosynthates via ectomycorrhizal hyphae to bacteria in root-distant soil areas. Fungal and bacterial phospholipid fatty acid (PLFA) biomarkers were significantly enriched in hyphae-exclusive compartments 24 h after 13C-CO2-labeling. Isotope imaging with nanometer-scale secondary ion mass spectrometry (NanoSIMS) allowed for the first time in situ visualization of plant-derived C and N taken up by an extraradical fungal hypha, and in microbial cells thriving on hyphal surfaces. When N was added to the litter compartments, bacterial biomass, and the amount of incorporated 13C strongly declined. Interestingly, this effect was also observed in adjacent soil compartments where added N was only available for bacteria through hyphal transport, indicating that ectomycorrhizal fungi were acting on soil bacteria. Together, our results demonstrate that (i) ectomycorrhizal hyphae rapidly transfer plant-derived C to bacterial communities in root-distant areas, and (ii) this transfer promptly responds to changing soil nutrient conditions.", "keywords": ["Hyphosphere priming", "DYNAMICS", "0301 basic medicine", "PLFAs", "Microbiology", "ectomycorrhiza", "03 medical and health sciences", "Mycorrhizosphere", "MICROBIAL COMMUNITY COMPOSITION", "NanoSIMS", "hyphal carbon transfer", "hyphosphere bacteria", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "IDENTIFICATION", "RHIZOSPHERE", "15. Life on land", "QR1-502", "EXTRACTION METHOD", "Ectomycorrhiza", "ORGANIC-MATTER", "MYCORRHIZAL FUNGI", "hyphosphere priming", "mycorrhizosphere", "Hyphal carbon transfer", "106022 Microbiology", "FATTY-ACIDS", "Hyphosphere bacteria", "BAYESIAN CLASSIFIER", "CARBON ALLOCATION"]}, "links": [{"href": "https://doi.org/11353/10.1146400"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11353/10.1146400", "name": "item", "description": "11353/10.1146400", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11353/10.1146400"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-26T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=ectomycorrhiza&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=ectomycorrhiza&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=ectomycorrhiza&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=ectomycorrhiza&offset=25", "hreflang": "en-US"}], "numberMatched": 25, "numberReturned": 25, "distributedFeatures": [], "timeStamp": "2026-05-31T06:54:24.399377Z"}