Haloalkane dehalogenases (HLDs) are \uffce\uffb1/\uffce\uffb2\uffe2\uff80\uff90hydrolases that convert halogenated compounds to their corresponding alcohols. The overall kinetic mechanism proceeds via four steps: (i) binding of halogenated substrate, (ii) bimolecular nucleophilic substitution (SN2) leading to the cleavage of a carbon\uffe2\uff80\uff90halogen bond and the formation of an alkyl\uffe2\uff80\uff90enzyme intermediate, (iii) nucleophilic addition of a water molecule resulting in the hydrolysis of the intermediate to the corresponding alcohol and (iv) release of the reaction products \uffe2\uff80\uff93 an alcohol, a halide ion and a proton. Although, the overall reaction has been reported as irreversible, several kinetic evidences from previous studies suggest the reversibility of the first SN2 chemical step. To study this phenomenon, we have engineered HLDs to stop the catalytic cycle at the stage of the alkyl\uffe2\uff80\uff90enzyme intermediate. The ability of the intermediate to exchange halides was confirmed by a stopped\uffe2\uff80\uff90flow fluorescence binding analysis. Finally, the transhalogenation reaction was confirmed with several HLDs and 2,3\uffe2\uff80\uff90dichloropropene in the presence of a high concentration of iodide. The formation of the transhalogenation product 3\uffe2\uff80\uff90iodo\uffe2\uff80\uff902\uffe2\uff80\uff90chloropropene catalysed by five mutant HLDs was identified by gas chromatography coupled with mass spectrometry. Hereby we demonstrated the reversibility of the cleavage of the carbon\uffe2\uff80\uff90halogen bond by HLDs resulting in a transhalogenation. After optimization, the transhalogenation reaction can possibly find its use in biocatalytic applications. Enabling this reaction by strategically engineering the enzyme to stop at an intermediate in the catalytic cycle that is synthetically more useful than the product of the natural pathway is a novel concept.
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", "keywords": ["0301 basic medicine", "enzyme catalysis; halide exchange; haloalkane dehalogenase; nucleophilic substitution; redirection of reaction; transhalogenation", "0303 health sciences", "03 medical and health sciences"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/adsc.201900132"}, {"href": "https://doi.org/10.1002/adsc.201900132"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Advanced%20Synthesis%20%26amp%3B%20Catalysis", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/adsc.201900132", "name": "item", "description": "10.1002/adsc.201900132", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/adsc.201900132"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-17T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2004.11.097", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:15:36Z", "type": "Journal Article", "created": "2005-01-27", "title": "Microbial Biomass And Enzyme Activities In Submerged Rice Soil Amended With Municipal Solid Waste Compost And Decomposed Cow Manure", "description": "We studied the suitability of municipal solid waste compost (MSWC) application to submerged rice paddies in the perspective of metal pollution hazards associated with such materials. Experiments were conducted during the wet seasons of 1997, 1998 and 1999 on rice grown under submerged condition, at the Agriculture Experimental Farm, Calcutta University at Baruipur, West Bengal, India. The treatments consisted of control, no input; MSWC, at 60 kgNha(-1); well decomposed cow manure (DCM), at 60 kgNha(-1); MSWC (30 kgNha(-1)) +Urea (30 kgNha(-1)); DCM (30 kgNha(-1)) +U (30 kgNha(-1)) and Fertilizer, (at 60:30:30 NPK kgha(-1) through urea, single superphosphate and muriate of potash respectively). Soil microbial biomass-C (MBC), MBC as percentage of organic-C (ratio index value, RIV), urease and acid phosphatase activities were higher in DCM than MSWC-treated soils, due to higher amount of biogenic organic materials like water soluble organic carbon, carbohydrate and mineralizable nitrogen in the former. The studied parameters were higher when urea was integrated with DCM or MSWC, compared to their single applications. Soil MBC, urease and acid phosphatase activities periodically declined up to 60 day after transplanting (DAT) and then increased after crop harvest. The heavy metals in MSWC did not detrimentally influence MBC, urease and acid phosphatase activities of soil. In the event of long term MSWC application, changes in soil quality parameters should be monitored regularly, since heavy metals once entering into soil persist over a long period.", "keywords": ["2. Zero hunger", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "6. Clean water", "Enzymes", "Refuse Disposal", "12. Responsible consumption", "Manure", "Soil", "13. Climate action", "11. Sustainability", "Animals", "0401 agriculture", " forestry", " and fisheries", "Cattle", "Biomass", "Fertilizers", "Soil Microbiology", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Ashis Kumar Chakraborty, Kalyan Chakrabarti, Pradip Bhattacharyya,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2004.11.097"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2004.11.097", "name": "item", "description": "10.1016/j.chemosphere.2004.11.097", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2004.11.097"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-07-01T00:00:00Z"}}, {"id": "10.1002/jsfa.4349", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:14:02Z", "type": "Journal Article", "created": "2011-03-29", "title": "Efficiency Of Urease And Nitrification Inhibitors In Reducing Ammonia Volatilization From Diverse Nitrogen Fertilizers Applied To Different Soil Types And Wheat Straw Mulching", "description": "Some authors suggest that the absence of tillage in agricultural soils might have an influence on the efficiency of nitrogen applied in the soil surface. In this study we investigate the influence of no-tillage and soil characteristics on the efficiency of a urease inhibitor (N-(n-butyl)thiophosphoric triamide, NBPT) and a nitrification inhibitor (diciandiamide, DCD) in decreasing ammonia volatilization from urea and ammonium nitrate (AN), respectively.The results indicate that ammonia volatilization in soils amended with urea was significantly higher than in those fertilized with AN. Likewise, the main soil factors affecting ammonia volatilization from urea are clay and sand soil contents. While clay impedes ammonia volatilization, sand favours it. The presence of organic residues on soil surface (no-tillage) tends to increase ammonia volatilization from urea, although this fact depended on soil type. The presence of NBPT in urea fertilizer significantly reduced soil ammonia volatilization. This action of NBPT was negatively affected by acid soil pH and favoured by soil clay content.The presence of organic residues on soil surface amended with urea increased ammonia volatilization, and was particularly high in sandy compared with clay soils. Application of NBPT reduced ammonia volatilization although its efficiency is reduced in acid soils. Concerning AN fertilization, there were no differences in ammonia volatilization with or without DCD in no-tillage soils.", "keywords": ["2. Zero hunger", "Nitrates", "Plant Stems", "Nitrogen", "Agriculture", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "Silicon Dioxide", "Nitrification", "Urease", "6. Clean water", "Soil", "Organophosphorus Compounds", "Ammonia", "Clay", "Urea", "0401 agriculture", " forestry", " and fisheries", "Aluminum Silicates", "Enzyme Inhibitors", "Volatilization", "Fertilizers", "Humic Substances", "Triticum"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.4349"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.4349", "name": "item", "description": "10.1002/jsfa.4349", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.4349"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-03-29T00:00:00Z"}}, {"id": "10.1007/978-3-030-21359-6_80", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:07Z", "type": "Report", "created": "2019-06-22", "title": "Soil Stabilization Against Water Erosion via Calcite Precipitation by Plant-Derived Urease", "description": "Stabilization is often necessary to improve the stiffness, strength and durability of both natural and compacted soils. Soils are typically stabilized via the addition of hydraulic binders, such as cement and lime, which present however relatively high carbon and energy footprints. Alternative stabilization techniques are therefore explored to minimize environmental impact while preserving good material properties. Among these techniques, enzyme induced calcite precipitation (EICP) has gained prominence in recent years. EICP exploits the action of the urease enzyme to catalyze the hydrolysis of urea and to produce carbonate ions, which then react with calcium ions inside the pore water to cause precipitation of calcium carbonate (i.e. calcite). The precipitated mineral bonds particles together, thus improving the hydro-mechanical characteristics of the soil. This paper presents a preliminary investigation on the use of plant-derived urease, instead of pure reagent-grade urease, to minimize environmental and financial costs. The urease enzyme is obtained from a liquid soybeans extract, inside which urea and calcium chloride are dissolved. This stabilizing solution is then mixed with a silty clay to produce stabilized samples, which are subjected to water erosion tests.", "keywords": ["2. Zero hunger", "Bio-cementation; Bio-mineralization; Enzyme induced calcite precipitation; Ground improvement; Plant-derived urease; Soil stabilization", "15. Life on land", "6. Clean water"]}, "links": [{"href": "https://iris.unige.it/bitstream/11567/997777/1/Cuccurullo%2c%20Gallipoli%2c%20Bruno%2c%20Augarde%2c%20Hughes%20and%20La%20Borderie%20%282019%29.pdf"}, {"href": "http://link.springer.com/content/pdf/10.1007/978-3-030-21359-6"}, {"href": "http://link.springer.com/content/pdf/10.1007/978-3-030-21359-6_80"}, {"href": "https://doi.org/10.1007/978-3-030-21359-6_80"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/978-3-030-21359-6_80", "name": "item", "description": "10.1007/978-3-030-21359-6_80", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/978-3-030-21359-6_80"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-23T00:00:00Z"}}, {"id": "10.1007/s00248-005-5156-y", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:14:16Z", "type": "Journal Article", "created": "2006-01-02", "title": "Microbial Responses To Long-Term N Deposition In A Semiarid Grassland", "description": "Nitrogen (N) enrichment of the biosphere is an expanding problem to which arid ecosystems may be particularly sensitive. In semiarid grasslands, scarce precipitation uncouples plant and microbial activities, and creates within the soil a spatial mosaic of rhizosphere and cyanobacterial crust communities. We investigated the impact of elevated N deposition on these soil microbial communities at a grama-dominated study site located incentral New Mexico (USA). The study plots were established in 1995 and receive 10 kg ha(-1) year(-1) of supplemental N in the form of NH(4)NO(3). Soil samples were collected in July 2004, following 2 years of severe drought, and again in March 2005 following a winter of record high precipitation. Soils were assayed for potential activities of 20 extracellular enzymes and N(2)O production. The rhizosphere and crust-associated soils had peptidase and peroxidase potentials that were extreme in relation to those of temperate soils. N addition enhanced glycosidase and phosphatase activities and depressed peptidase. In contrast to temperate forest soils, oxidative enzyme activity did not respond to N treatment. Across sampling dates, extracellular enzyme activity responses correlated with inorganic N concentrations. N(2)O generation did not vary significantly with soil cover or N treatment. Microbial responses to N deposition in this semiarid grassland were distinct from those of forest ecosystems and appear to be modulated by inorganic N accumulation, which is linked to precipitation patterns.", "keywords": ["2. Zero hunger", "Principal Component Analysis", "Ecology", "Nitrogen", "Climate", "beta-Glucosidase", "Nitrous Oxide", "04 agricultural and veterinary sciences", "15. Life on land", "Poaceae", "01 natural sciences", "Carbon", "6. Clean water", "Enzymes", "Trees", "Leucyl Aminopeptidase", "Soil", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Soil Microbiology", "Peroxidase", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00248-005-5156-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-005-5156-y", "name": "item", "description": "10.1007/s00248-005-5156-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-005-5156-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-01-01T00:00:00Z"}}, {"id": "10.1007/s00267-012-9890-x", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:14:17Z", "type": "Journal Article", "created": "2012-06-28", "title": "Changes In Labile Organic Carbon Fractions And Soil Enzyme Activities After Marshland Reclamation And Restoration In The Sanjiang Plain In Northeast China", "description": "The extensive reclamation of marshland into cropland has tremendously impacted the ecological environment of the Sanjiang Plain in northeast China. To understand the impacts of marshland reclamation and restoration on soil properties, we investigated the labile organic carbon fractions and the soil enzyme activities in an undisturbed marshland, a cultivated marshland and three marshlands that had been restored for 3, 6 and 12 years. Soil samples collected from the different management systems at a depth of 0-20 cm in July 2009 were analyzed for soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass carbon (MBC) and easily degradable organic carbon. In addition, the activities of the invertase, \u03b2-glucosidase, urease and acid phosphatase were determined. These enzymes are involved in C, N and P cycling, respectively. Long-term cultivation resulted in decreased SOC, DOC, MBC, microbial quotient and C (invertase, \u03b2-glucosidase) and N-transforming (urease) enzyme activities compared with undisturbed marshland. After marshland restoration, the MBC and DOC concentrations and the soil invertase, \u03b2-glucosidase and urease activities increased. Soil DOC and MBC concentrations are probably the main factors responsible for the different invertase, \u03b2-glucosidase and urease activities. In addition, marshland restoration caused a significant increase in the microbial quotient, which reflects enhanced efficiency of organic substrate use by microbial biomass. Our observations demonstrated that soil quality recovered following marshland restoration. DOC, MBC and invertase, \u03b2-glucosidase and urease activities were sensitive for discriminating soil ecosystems under the different types of land use. Thus, these parameters should be considered to be indicators for detecting changes in soil quality and environmental impacts in marshlands.", "keywords": ["2. Zero hunger", "China", "Conservation of Natural Resources", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "6. Clean water", "Enzymes", "Soil", "13. Climate action", "Wetlands", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00267-012-9890-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00267-012-9890-x", "name": "item", "description": "10.1007/s00267-012-9890-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00267-012-9890-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-06-29T00:00:00Z"}}, {"id": "10.1007/s00374-010-0462-z", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:20Z", "type": "Journal Article", "created": "2010-04-26", "title": "The Effect Of Different Tree Species On The Chemical And Microbial Properties Of Reclaimed Mine Soils", "description": "The chemical and microbial properties of afforested mine soils are likely to depend on the species composition of the introduced vegetation. This study compared the chemical and microbial properties of organic horizons and the uppermost mineral layers in mine soils under pure pine (Pinus sylvestris), birch (Betula pendula), larch (Larix decidua), alder (Alnus glutinosa), and mixed pine\u2013alder and birch\u2013alder forest stands. The studied properties included soil pH, content of organic C (Corg) and total N (Nt), microbial biomass (Cmic), basal respiration, nitrogen mineralization rate (Min-N), and the activities of dehydrogenase, acid phosphomonoesterase, and urease. Near-infrared spectroscopy (NIR) was used to detect differences in the chemical composition of soil organic matter under the studied forest stands. There were significant differences in Corg and Nt contents between stands in both O and mineral soil horizons and also in the chemical composition of the accumulated organic matter, as indicated by NIR spectra differences. Alder was associated with the largest Corg and Nt accumulation but also with a significant decrease of pH in the mineral soil. Microbial biomass, respiration, the percentage of Corg present as Cmic, Min-N, and dehydrogenase activity were the highest under the birch stand, indicating a positive effect of birch on soil microflora. Admixture of alder to coniferous stand increased basal respiration, Min-N, and activities of dehydrogenase and acid phosphomonoesterase as compared with the pure pine stand. In the O horizon, soil pH and Nt content had the most important effects on all microbial properties. In this horizon, the activities of urease and acid phosphomonoesterase did not depend on microbial biomass. In the mineral layer, however, the amount of accumulated C and microbial biomass were of primary importance for the enzyme activities.", "keywords": ["microbial biomass", "13. Climate action", "soil enzyme activities", "NIR spectroscopy", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "mine soils", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00374-010-0462-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00374-010-0462-z", "name": "item", "description": "10.1007/s00374-010-0462-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00374-010-0462-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-04-27T00:00:00Z"}}, {"id": "10.1007/s00374-012-0761-7", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:21Z", "type": "Journal Article", "created": "2012-12-10", "title": "Short-Term Effects Of Organic And Inorganic Fertilizers On Soil Microbial Community Structure And Function", "description": "Open AccessA field study was carried out to analyze the short-term impacts of replacing mineral by organic fertilizers on the microbial and biochemical parameters relevant for soil fertility and crop yield. Three types of fertilization regimes were compared: (1) conventional fertilizer regime with inorganic fertilizer, and combined integrated fertilizer regimes in which 25 % of the nutrients were supplied by either (2) rabbit manure or (3) vermicompost. The effects on microbial community structure and function (phospholipid fatty acid [PLFA] profiles, bacterial growth, fungal growth, basal respiration, \u03b2-glucosidase, protease and phosphomonoesterase activities), soil biochemical properties (total C, dissolved organic carbon [DOC], N-NH4 +, N-NO3 \u2212, PO4, total K) and crop yield were investigated in the samples collected from the experimental soil at harvest, 3 months after addition of fertilizer. The integrated fertilizer regimes stimulated microbial growth, altered the structure of soil microbial community and increased enzyme activity relative to inorganic fertilization. Bacterial growth was particularly influenced by the type of fertilizer regime supplied, while fungal growth only responded to the amount of fertilizer provided. The use of manure produced a fast increase in the abundance of PLFA biomarkers for Gram-negative bacteria as compared to inorganic fertilizer. Nutrient supply and crop yield with organic fertilizers were maintained at similar levels to those obtained with inorganic fertilizer. The effects of the organic amendments were observed even when they involved a small portion of the total amount of nutrients supplied; thereby confirming that some of the beneficial effects of integrated fertilizer strategies may occur in the short term.", "keywords": ["Manure", "2. Zero hunger", "2511 Ciencias del Suelo (Edafolog\u00eda)", "13. Climate action", "Sustainable agriculture", "0401 agriculture", " forestry", " and fisheries", "Soil enzymes", "PLFAs", "04 agricultural and veterinary sciences", "15. Life on land", "Vermicompost", "6. Clean water", "Organic fertilizers"]}, "links": [{"href": "https://doi.org/10.1007/s00374-012-0761-7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00374-012-0761-7", "name": "item", "description": "10.1007/s00374-012-0761-7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00374-012-0761-7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-12-11T00:00:00Z"}}, {"id": "10.1007/s00442-012-2578-3", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:25Z", "type": "Journal Article", "created": "2013-01-07", "title": "Effects Of Drought And N-Fertilization On N Cycling In Two Grassland Soils", "description": "Open AccessOecologia, 171 (3)", "keywords": ["[SDE] Environmental Sciences", "N2O fluxes", "550", "functional genes", "Nitrogen", "[SDV]Life Sciences [q-bio]", "Climate", "Climate Change", "Nitrification and denitrification", "enzyme activites", "Urine", "630", "10127 Institute of Evolutionary Biology and Environmental Studies", "Soil", "Quantitative PCR", "Climate change; Enzyme activities; Functional genes; Quantitative PCR; Nitrification and denitrification; N2O fluxes", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Animals", "Climate change", "Enzyme activities", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "Ecosystem", "Soil Microbiology", "Functional genes", "Nitrogen Cycle", "Plants", "Archaea", "Droughts", "[SDV] Life Sciences [q-bio]", "1105 Ecology", " Evolution", " Behavior and Systematics", "climate change", "Genes", " Bacterial", "[SDE]Environmental Sciences", "quantitative PCR", "Denitrification", "570 Life sciences; biology", "590 Animals (Zoology)", "Cattle", "nitrification and denitrification"]}, "links": [{"href": "https://doi.org/10.1007/s00442-012-2578-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-012-2578-3", "name": "item", "description": "10.1007/s00442-012-2578-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-012-2578-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-01-08T00:00:00Z"}}, {"id": "10.1007/s10021-020-00512-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:30Z", "type": "Journal Article", "created": "2020-05-21", "title": "Effects of Litter Quality Diminish and Effects of Vegetation Type Develop During Litter Decomposition of Two Shrub Species in an Alpine Treeline Ecotone", "description": "Because climate change is predicted to have a strong impact on high-altitude ecosystems, a better knowledge of litter decomposition in alpine ecosystems is critical to improve our predictions of the effect of climate change on ecosystem processes and services such as nutrient cycling, carbon sequestration, and below-ground biodiversity. To evaluate the effects of vegetation types [alpine shrubland (AS) and alpine meadow (AM)] and litter quality on litter decomposition and related biochemical processes, the decomposition of leaf litter of two dominant shrub species, Sorbus rufopilosa (SR, high quality) and Rhododendron lapponicum (RL, low quality), was studied using the litterbag method in an alpine treeline ecotone on the eastern Tibetan Plateau. After 1 year of decomposition, cellulolytic enzyme activities and gram-negative bacterial biomass were higher in shrubland than in meadow. However, higher fungal biomass, fungal/bacteria ratio and ligninolytic activity were observed in meadow than in shrubland after 2 years of decomposition. During the first year of decomposition, litter decomposition was faster in shrubland than in meadow probably due to the home-field advantage (HFA) effect and the bacteria-dominated decomposition, whereas in later decomposition stages, litter decomposition was faster in meadow than in shrubland, as the HFA effect diminished and fungal-dominated decomposition of recalcitrant components took over. These results indicated that litter quality effects were generally strongest in the first year and diminished in later stages when the effect of vegetation type in incubation sites developed.", "keywords": ["Lignocellulolytic enzyme", "2. Zero hunger", "0106 biological sciences", "Litter quality", "Litter decomposition", "500", "15. Life on land", "Soil carbon", "01 natural sciences", "Alpine treeline ecotone", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "13. Climate action", "Microbial community", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology"]}, "links": [{"href": "https://doi.org/10.1007/s10021-020-00512-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10021-020-00512-9", "name": "item", "description": "10.1007/s10021-020-00512-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10021-020-00512-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-21T00:00:00Z"}}, {"id": "10.1007/s10533-004-7112-1", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:35Z", "type": "Journal Article", "created": "2005-11-04", "title": "Extracellular Enzyme Activities And Soil Organic Matter Dynamics For Northern Hardwood Forests Receiving Simulated Nitrogen Deposition", "description": "Anthropogenic nitrogen enrichment alters decomposition processes that control the flux of carbon (C) and nitrogen (N) from soil organic matter (SOM) pools. To link N-driven changes in SOM to microbial responses, we measured the potential activity of several extracellular enzymes involved in SOM degradation at nine experimental sites located in northern Michigan. Each site has three treatment plots (ambient, +30 and +80 kg N ha 1 y 1 ). Litter and soil samples were collected on five dates over the third growing season of N treatment. Phenol oxidase, peroxidase and cellobiohydrolase activities showed significant responses to N additions. In the Acer saccha- rum-Tilia americana ecosystem, oxidative activity was 38% higher in the litter horizon of high N treatment plots, relative to ambient plots, while oxidative activity in mineral soil showed little change. In the A. saccharum-Quercus rubra and Q. velutina-Q. alba ecosystems, oxidative activities declined in both litter (15 and 23%, respectively) and soil (29 and 38%, respectively) in response to high N treatment while cellobiohydrolase activity increased (6 and 39% for litter, 29 and 18% for soil, respectively). Over 3 years, SOM content in the high N plots has decreased in the Acer-Tilia ecosystem and increased in the two Quercus ecosystems, relative to ambient plots. For all three ecosystems, differences in SOM content in relation to N treatment were directly related (r 2 = 0.92) to an enzyme activity factor that included both oxidative and hydrolytic enzyme responses.", "keywords": ["Soil Science & Conservation", "Decomposition", "Science", "Ecology and Evolutionary Biology", "Terrestrial Pollution", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "15. Life on land", "Biochemistry", "Phenol Oxidase", "Geochemistry", "Cellulase", "Soil Organic Matter", "Health Sciences", "0401 agriculture", " forestry", " and fisheries", "Nitrogen Deposition", "Cellular and Developmental Biology", "General", "Extracellular Enzyme Activity", "Geosciences"]}, "links": [{"href": "https://doi.org/10.1007/s10533-004-7112-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10533-004-7112-1", "name": "item", "description": "10.1007/s10533-004-7112-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10533-004-7112-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-08-01T00:00:00Z"}}, {"id": "10.1007/s10533-008-9257-9", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:14:36Z", "type": "Journal Article", "created": "2008-11-11", "title": "Nitrogen Deposition Effects On Soil Organic Matter Chemistry Are Linked To Variation In Enzymes, Ecosystems And Size Fractions", "description": "Recent research has dramatically advanced our understanding of soil organic matter chemistry and the role of N in some organic matter transformations, but the effects of N deposition on soil C dynamics remain difficult to anticipate. We examined soil organic matter chemistry and enzyme kinetics in three size fractions (>250 \u03bcm, 63\u2013250 \u03bcm, and 250 \u03bcm fraction of the sugar maple\u2013basswood ecosystem from 0.9 to 3.3 but there were no changes in other size classes or in the black oak\u2013white oak ecosystem. Third, simulated N deposition increased the ratio of lignin derivatives to N-bearing compounds in the 63\u2013250 and >250 \u03bcm fractions in both ecosystems but not in the 63 \u03bcm) where there were multiple correlations between oxidative enzyme activities and concentrations of lignin derivatives and between glycanolytic enzyme activities and concentrations of carbohydrates. Within silt-clay fractions (<63 \u03bcm), these enzyme-substrate correlations were attenuated by interactions with particle surfaces. Our results demonstrate that variation in enzyme activity resulting from atmospheric N deposition is directly linked to changes in soil organic matter chemistry, particularly those that occur within coarse soil size fractions.", "keywords": ["Carbon structure", "Soil organic matter", "13. Climate action", "Pyrolysis gas chromatography/mass spectrometry", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Biogeochemistry", "15. Life on land", "Nitrogen deposition", "Enzymes"]}, "links": [{"href": "https://doi.org/10.1007/s10533-008-9257-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10533-008-9257-9", "name": "item", "description": "10.1007/s10533-008-9257-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10533-008-9257-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-10-01T00:00:00Z"}}, {"id": "10.1007/s10533-015-0157-5", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:37Z", "type": "Journal Article", "created": "2015-11-14", "title": "Chronic Nitrogen Fertilization And Carbon Sequestration In Grassland Soils: Evidence Of A Microbial Enzyme Link", "description": "Chronic nitrogen (N) fertilization can greatly affect soil carbon (C) sequestration by altering biochemical interactions between plant detritus and soil microbes. In lignin-rich forest soils, chronic N additions tend to increase soil C content partly by decreasing the activity of lignin-degrading enzymes. In cellulose-rich grassland soils it is not clear whether cellulose-degrading enzymes are also inhibited by N additions and what consequences this might have on changes in soil C content. Here we address whether chronic N fertilization has affected (1) the C content of light versus heavier soil fractions, and (2) the activity of four extracellular enzymes including the C-acquiring enzyme \u03b2-1,4-glucosidase (BG; necessary for cellulose hydrolysis). We found that 19\u00a0years of chronic N-only addition to permanent grassland have significantly increased soil C sequestration in heavy but not in light soil density fractions, and this C accrual was associated with a significant increase (and not decrease) of BG activity. Chronic N fertilization may increase BG activity because greater N availability reduces root C:N ratios thus increasing microbial demand for C, which is met by C inputs from enhanced root C pools in N-only fertilized soils. However, BG activity and total root mass strongly decreased in high pH soils under the application of lime (i.e. CaCO3), which reduced the ability of these organo-mineral soils to gain more C per units of N added. Our study is the first to show a potential \u2018enzyme link\u2019 between (1) long-term additions of inorganic N to grassland soils, and (2) the greater C content of organo-mineral soil fractions. Our new hypothesis is that the \u2018enzyme link\u2019 occurs because (a) BG activity is stimulated by increased microbial C demand relative to N under chronic fertilization, and (b) increased BG activity causes more C from roots and from microbial metabolites to accumulate and stabilize into organo-mineral C fractions. We suggest that any combination of management practices that can influence the BG \u2018enzyme link\u2019 will have far reaching implications for long-term C sequestration in grassland soils.", "keywords": ["DECOMPOSITION", "DYNAMICS", "570", "\u03b2-1", "4-Glucosidase", "/dk/atira/pure/subjectarea/asjc/2300/2304", "NUTRIENT RELEASE", "Environmental Sciences & Ecology", "Root C:N ratio", "Extracellular enzyme activity", "LITTER DECAY", "FOREST ECOSYSTEMS", "0399 Other Chemical Sciences", "0402 Geochemistry", "Environmental Chemistry", "Geosciences", " Multidisciplinary", "beta-1", "4-Glucosidase", "Earth-Surface Processes", "Water Science and Technology", "2. Zero hunger", "Multidisciplinary", "Science & Technology", "/dk/atira/pure/subjectarea/asjc/1900/1904", "Geology", "sequestration", "Agronomy & Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Soil carbon", "N DEPOSITION", "ORGANIC-MATTER", "PHOSPHORUS", "Fertilization", "Physical Sciences", "N ratio [Root C]", "0401 agriculture", " forestry", " and fisheries", "Soil carbon sequestration", "Liming", "TURNOVER", "Life Sciences & Biomedicine", "Geosciences", "/dk/atira/pure/subjectarea/asjc/2300/2312", "Environmental Sciences", "RESPONSES"]}, "links": [{"href": "https://doi.org/10.1007/s10533-015-0157-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10533-015-0157-5", "name": "item", "description": "10.1007/s10533-015-0157-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10533-015-0157-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-11-14T00:00:00Z"}}, {"id": "10.1007/s10533-020-00728-w", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:37Z", "type": "Journal Article", "created": "2020-11-19", "title": "How will a drier climate change carbon sequestration in soils of the deciduous forests of Central Europe?", "description": "AbstractGlobal warming is accompanied by increasing water stress across much of our planet. We studied soil biological processes and changes in soil organic carbon (SOC) storage in 30 Hungarian oak forest sites in the Carpathian Basin along a climatic gradient (mean annual temperature (MAT) 9.6\uffe2\uff80\uff9312.1\uffc2\uffa0\uffc2\uffb0C, mean annual precipitation (MAP) 545\uffe2\uff80\uff93725\uffc2\uffa0mm) but on similar gently sloped hillsides where the parent materials are loess and weathered dust inputs dating from the end of the ice age. The purpose of this research was to understand how a drying climate, predicted for this region, might regulate long-term SOC sequestration. To examine the effects of decreasing water availability, we compared soil parameters and processes in three categories of forest that represented the moisture extremes along our gradient and that were defined using a broken-stick regression model. Soil biological activity was significantly lower in the driest (\uffe2\uff80\uff9cdry\uffe2\uff80\uff9d) forests, which had more than double the SOC concentration in the upper 30\uffc2\uffa0cm layer (3.28\uffc2\uffa0g C/100\uffc2\uffa0g soil\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff890.11 SE) compared to soils of the wettest (\uffe2\uff80\uff9chumid\uffe2\uff80\uff9d) forests (1.32\uffc2\uffa0g C/100\uffc2\uffa0g soil\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff890.09 SE), despite the fact that annual surface litter production in humid forests was\uffe2\uff80\uff89~\uffe2\uff80\uff8937% higher than in dry forests. A two-pool SOM model constrained to fit radiocarbon data indicates that turnover times for fast and slow pools are about half as long in the humid soil compared to the dry soil, and humid soils transfer C twice as efficiently from fast to slow pools. Enzyme activity and fungal biomass data also imply shorter turnover times associated with faster degradation processes in the soils of humid forests. Thermogravimetry studies suggest that more chemically recalcitrant compounds are accumulating in the soils of dry forests. Taken together, our results suggest that the predicted climate drying in this region might increase SOC storage in Central European mesic deciduous forests even as litter production decreases.50% higher in the organic than the inorganic fertilizer-based system (P < 0.05). Crop yields in 2008 were generally lower in the low-input organic rotations than in the high-input inorganic fertilizer-based system; only spring barley in rotations with pig slurry application and incorporation of a catch crop prior to sowing obtained grain yields similar to levels achieved in the system where inorganic fertilizer was applied. These results suggest that within organic cropping systems, both microbial activity and crop yields could be enhanced through inclusion of catch crops. However, the timing of catch crop incorporation is critical.", "keywords": ["2. Zero hunger", "microbial biomass", "Nutrient turnover", "inorganic fertilizer", "15. Life on land", "potential ammonium oxidation", "Air and water emissions", "6. Clean water", "12. Responsible consumption", "denitrifier enzyme activity", "Soil biology", "/dk/atira/pure/core/keywords/Life", "13. Climate action", "potential mineralizable nitrogen", "catch drop", "gas diffusivity", "11. Sustainability", "Former LIFE faculty"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2010.10.001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2010.10.001", "name": "item", "description": "10.1016/j.agee.2010.10.001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2010.10.001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-12-01T00:00:00Z"}}, {"id": "10.1007/s11104-017-3235-8", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:52Z", "type": "Journal Article", "created": "2017-03-29", "title": "Responses Of Soil Extracellular Enzyme Activities To Experimental Warming And Co2 Enrichment At The Alpine Treeline", "description": "Climate warming and elevated CO2 can modify nutrient cycling mediated by enzymes in soils, especially in cold-limited ecosystems with a low availability of nutrients and a high temperature sensitivity of decomposition and mineralization. We estimated responses of soil extracellular enzyme activities (EEAs) to 6\u00a0years of soil warming and 9\u00a0years of CO2 enrichment at an Alpine treeline site. EEAs were measured in the litter (L), fermentation (F) and humified (H) horizons under Larix decidua and Pinus uncinata trees. Soil warming indirectly affected EEAs through altered soil moisture, fine root biomass, and C:N ratio of the organic horizons. Warming increased \u03b2-glucosidase and \u03b2-xylosidase activities in the F horizon but led to reduced laccase activity in the L horizon, probably caused by drying of the litter horizon associated with the treatment. In the H horizon, previous CO2 enrichment altered the activity of leucine amino peptidase, N-acetylglucosaminidase, and phosphatase. No interactive effects between warming and CO2 enrichment were detected. Warming affected the temperature sensitivity of \u03b2-xylosidase but not of the other enzymes. Altered EEAs after six years of soil warming indicate a sustained stimulation of carbon, nitrogen and nutrient cycling under climatic warming at the alpine treeline.", "keywords": ["0106 biological sciences", "High Temperature", "Nutrient Cycling", "Climate Change", "Larix Decidua", "Fine Root", "04 agricultural and veterinary sciences", "Alpine Environment", "Carbon Dioxide", "15. Life on land", "01 natural sciences", "Treeline", "Enzyme Activity", "10122 Institute of Geography", "Coniferous Tree", "Pinus Uncinata", "13. Climate action", "Fermentation", "1110 Plant Science", "0401 agriculture", " forestry", " and fisheries", "Global Change", "Warming", "910 Geography & travel", "1111 Soil Science"]}, "links": [{"href": "https://doi.org/10.1007/s11104-017-3235-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-017-3235-8", "name": "item", "description": "10.1007/s11104-017-3235-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-017-3235-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-03-29T00:00:00Z"}}, {"id": "10.1007/s11104-022-05340-5", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:14:53Z", "type": "Journal Article", "created": "2022-03-22", "title": "The role of microbes in the increase of organic phosphorus availability in the rhizosheath of cover crops", "description": "AbstractBackground and aims
The characterisation of plant-available phosphorus (P) pools and the assessment of the microbial community in the rhizosheath of cover crops can improve our understanding of plant\uffe2\uff80\uff93microbe interactions and P availability.
MethodsMustard (Sinapis alba), phacelia (Phacelia tanacetifolia) and buckwheat (Fagopyrum esculentum) were grown as cover crops before soybean (Glycine max) in an on-farm experiment on a soil low in available P in southwest Germany. The cycling of P through the cover crop biomass and the enzyme-availability of organic P (Porg) pools in the cover crop rhizosheath were characterised. The soil microbial community (PLFA), activity (acid and alkaline phosphomonoesterase, as well as phosphodiesterase), and microbial P were assessed. The abundance of 16S-rRNA andphoD, coding for alkaline phosphomonoesterase in bacteria, were quantified using real-time qPCR.
ResultsMustard contained the greatest amount of P in its large biomass. In the rhizosheath of all cover crops, the concentration of enzyme-labile Porgwas higher than that in the control bulk soil, along with substantial increases of microbial abundance and activity. There were little differences among cover crop species, few changes in the bulk soil and only a limited carryover effect to soybean, except for fungi.
ConclusionsTurnover of microbial biomass, especially saprotrophic fungi, increased by rhizodeposition of cover crop roots; this was likely responsible for the observed increases in enzyme-available Porg. Microbial function was correlated linearly with microbial biomass, and the data of enzyme activity andphoDdid not suggest a difference of their specific activity between bulk and rhizosheath soil.
\u2022 Reduced oxygen enhanced microbial specific C-, N- and P-acquiring enzyme activity.
\u2022 Reduced oxygen increased microbial C relative to N and P limitation. \u2022 Reduced oxygen increased microbial N relative to P limitation. \u2022 Specific enzyme activity was positively related to qCO2 under reduced oxygen.
Mangroves are one of the most ecologically sensitive ecosystems to global climate change, which have cascading impacts on soil carbon (C), nitrogen (N) and phosphorus (P) cycling. Moreover, mangroves are experiencing increasing N and P loadings and reduced oxygen availability due to intensified climate change and human activities. However, both direct and interactive effects of these perturbations on microbially mediated soil C, N and P cycling are poorly understood. Here, we simultaneously investigated the effects of N and P loadings and reduced oxygen on microbial biomass, microbial respiration, and extracellular enzyme activities (EEAs) in mangrove soils. We calculated the microbial metabolic quotient (qCO2), which is regarded as a useful inverse metric of microbial C use efficiency (CUE). Our results show that reduced oxygen significantly increases both qCO2 and microbial specific EEAs (enzyme activity per unit of microbial biomass) for C-, N- and P-acquisition regardless of N or P loadings. Furthermore, we found that qCO2 positively correlated with microbial specific EEAs under reduced oxygen, whereas no clear relationship was detected under ambient oxygen. These results suggest that reduced oxygen increases microbial specific EEAs at the expense of increasing microbial respiration per unit biomass, indicating higher energy cost per unit enzyme production.", "keywords": ["mangrove", "nutrient acquisition", "microbial respiration", "nutrient addition", "13. Climate action", "extracellular enzyme", "0401 agriculture", " forestry", " and fisheries", "reduced oxygen", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s42832-022-0157-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Ecology%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s42832-022-0157-z", "name": "item", "description": "10.1007/s42832-022-0157-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s42832-022-0157-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-29T00:00:00Z"}}, {"id": "10.1016/j.agee.2011.06.015", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-07-26T16:15:11Z", "type": "Journal Article", "created": "2011-07-29", "title": "Short Term Effects Of Bioenergy By-Products On Soil C And N Dynamics, Nutrient Availability And Biochemical Properties", "description": "Abstract The shift towards a biobased economy will probably trigger the application of bioenergy by-products to the soil as either amendments or fertilizers. However, limited research has been done to determine how this will influence C and N dynamics and soil functioning. The aim of this work was to investigate the effects of different bioenergy by-products on C and N mineralisation, nutrient availability and microbial content and activity of amended soil and compare them to other more commonly used organic amendments. Two agricultural soils were amended (0.5% w/w) with four different bioenergy by-products (anaerobic digestate, rapeseed meal, bioethanol residue, biochar) and three other commonly used organic amendments (sewage sludge and two composts) and incubated at 20\u00a0\u00b0C in the laboratory for 30 days. During incubation, soil CO2 and N2O evolution were measured every 4\u00a0h by an automatic chromatographic system. After 2, 7 and 30 days of incubation, soil samples were analysed for K2SO4\u2013extractable C, N, NO3\u2212, NH4+ and P, microbial biomass C and three enzymatic activities (\u03b2-glucosidase, alkaline phosphatase and leucine aminopeptidase). Soil amendment led to a general increase in soil respiration, available N and P and microbial content and activity, but with remarkably different dynamics and values. Particularly, rapeseed meal and the bioethanol by-product led to N2O emissions and the greatest increases in soil respiration, N availability and enzymatic activity compared with the other amendments. The exception was represented by biochar that did not cause any significant variation with respect to the control, but promoted C accumulation. According to their impact on soil biochemical properties, the materials can be ranked as follows: rapeseed meal, bioethanol residue\u00a0>\u00a0anaerobic digestate, sewage sludge\u00a0>\u00a0composts\u00a0>\u00a0biochar. For each measured parameter, soil properties did not affect the response pattern found for the different treatments, but modified the magnitude of the response. In particular, soil respiration and enzymatic activity were higher in the slightly acidic soil, while greater values of available P were found in the alkaline soil. This study clearly indicates that the impact on GHG emissions and soil functioning of bioenergy by-products needs to be taken into account for a correct life cycle assessment of the bioenergy chain. Moreover, when properly managed, they may represent an effective alternative to usual amendments to improve the quality and nutrient balance of amended soils.", "keywords": ["2. Zero hunger", "crop residues", "decomposition", "microbial biomass", "carbon", "enzyme-activities", "04 agricultural and veterinary sciences", "15. Life on land", "composts", "7. Clean energy", "6. Clean water", "12. Responsible consumption", "organic amendments", "13. Climate action", "nitrous-oxide emission", "0401 agriculture", " forestry", " and fisheries", "mineralization", "management"], "contacts": [{"organization": "Galvez, A., Sinicco, T., Cayuela, M.L, Mingorance, M.D., Fornasier, F., Mondini, C.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2011.06.015"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2011.06.015", "name": "item", "description": "10.1016/j.agee.2011.06.015", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2011.06.015"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-10-01T00:00:00Z"}}, {"id": "10.1016/j.agrformet.2025.110503", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-07-26T16:15:19Z", "type": "Journal Article", "created": "2025-03-18", "title": "Linking soil extracellular enzymes with soil respiration under altered litter inputs", "description": "
Climate and land-use changes have altered both litter quality and quantity, with cascading impacts on soil respiration (SR). Soil extracellular enzymes (EEs) like cellulase and ligninase are crucial for deconstructing plant litter because they convert polymers into monomers. However, whether and how changes in litter inputs influence soil cellulase and ligninase activities as well as the implications for SR remain poorly understood. We conducted a global meta-analysis of 827 observations on the responses of SR and soil cellulase and ligninase activities to litter addition and litter removal. Litter addition significantly increased cellulase activity by 25 %, whereas litter removal decreased it by 26 %. Neither litter addition nor litter removal affected ligninase activity. Changes in cellulase activity correlated positively with SR under both litter addition and litter removal, but no such relationship was found for ligninase activity. These results indicate that changes in litter inputs affect SR primarily by affecting the microbial decomposition of readily decomposable rather than more structurally complex carbon pools. In addition, the effects of changes in litter inputs on cellulase activity decreased with treatment duration, suggesting that the long-term effects of changes in litter inputs on SR might be smaller than previously thought. Our results underscore the dominant role of cellulase in mediating the responses of SR to altered litter inputs. Integrating cellulase responses to altered litter inputs into Earth system models could improve the representation of microbial processes and refine the predictions of soil carbon dynamics.
", "keywords": ["Soil carbon pools", "Litter alternations", "Soil respiration", "Global changes", "Soil extracellular enzymes", "Soil microorganisms"]}, "links": [{"href": "https://doi.org/10.1016/j.agrformet.2025.110503"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agricultural%20and%20Forest%20Meteorology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agrformet.2025.110503", "name": "item", "description": "10.1016/j.agrformet.2025.110503", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agrformet.2025.110503"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-05-01T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2013.10.009", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:15:25Z", "type": "Journal Article", "created": "2013-11-30", "title": "Microplate-Scale Fluorometric Soil Enzyme Assays As Tools To Assess Soil Quality In A Long-Term Agricultural Field Experiment", "description": "Abstract We investigated the potential of microplate-scale fluorometric soil enzyme assays to differentiate plots under contrasting long-term organic and mineral N fertilization regimens to determine the relevance of this analytical approach to soil quality related studies. Enzymes involved in the breakdown of cellulose and hemicellulose showed maximum activities in plots amended with manure. Conversely, the enzymes involved in the hydrolysis of starch and phosphate esters peaked under mineral N fertilization. Linear regression analysis indicated close associations between enzyme activities and other fundamental soil properties related to soil quality, and principal component analysis separated the soil samples according to their responses to organic and mineral N fertilization. We conclude that microplate-scale fluorimetry is a fast throughput tool for the measurement of multiple soil enzyme activities as soil quality indicators.", "keywords": ["2. Zero hunger", "Soil enzyme activities", " Microplate-scale fluorimetry", " 4-Methylumbelliferone", " Soil quality", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2013.10.009"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2013.10.009", "name": "item", "description": "10.1016/j.apsoil.2013.10.009", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2013.10.009"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-03-01T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2010.08.002", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:25Z", "type": "Journal Article", "created": "2010-08-31", "title": "Effect Of Texture And Tree Species On Microbial Properties Of Mine Soils", "description": "Reestablishment of soil microbial communities is a prerequisite for successful reclamation of post-mining barrens. The objective of this study was to assess the effect of texture of soil substrate and the planted tree species on microbial properties of mine soils reclaimed for forestry. Soil samples were taken from loamy sands and sands afforested with Scots pine and silver birch either in monocultures or in the mixed stands. The samples were measured for the contents of organic C (Corg), total N (Nt) and pH. The examined microbial properties included basal respiration (RESP), microbial biomass (Cmic), Cmic-to-Corg ratio, activities of dehydrogenase, acid phosphomonoesterase and urease and community level physiological profiles (CLPPs) studied using Biolog\u00ae Ecoplates. The loamy sands had higher pH, contained more Corg, Nt and Cmic and exhibited higher basal respiration and enzyme activities than the sands. However, their Cmic-to-Corg ratio was lower indicating less availability of Corg for soil microbes compared with the sands. The CLPPs in the loamy sands differed from those in the sands although there was no difference in microbial diversity (expressed as Shannon's diversity index) and activity on the Biolog\u00ae plates between the two textural classes. Tree species did not affect Corg, Nt and Corg-to-Nt ratio and had only a weak effect on CLPPs. However, the values of Cmic, RESP, Cmic-to-Corg ratio, dehydrogenase and urease activities were significantly lower under pine compared with the birch and mixed stands. The obtained results suggest that the texture of soil substrate is of higher importance for microbial properties of the studied mine soils than the planted vegetation.", "keywords": ["tree species", "microbial biomass", "enzyme activities", "CLPP", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "mine soils", "soils texture"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2010.08.002"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2010.08.002", "name": "item", "description": "10.1016/j.apsoil.2010.08.002", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2010.08.002"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-10-01T00:00:00Z"}}, {"id": "10.1016/j.ecolind.2018.03.034", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:42Z", "type": "Journal Article", "created": "2018-03-26", "title": "Grazing reduces the capacity of Landscape Function Analysis to predict regional-scale nutrient availability or decomposition, but not total nutrient pools", "description": "Open AccessPeer reviewed", "keywords": ["2. Zero hunger", "0106 biological sciences", "Aridity; Enzyme activities; Carbon; Nitrogen; Phosphorus; Drylands", "13. Climate action", "XXXXXX - Unknown", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.ecolind.2018.03.034"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecological%20Indicators", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ecolind.2018.03.034", "name": "item", "description": "10.1016/j.ecolind.2018.03.034", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ecolind.2018.03.034"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-01T00:00:00Z"}}, {"id": "10.1016/j.biortech.2017.03.095", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:32Z", "type": "Journal Article", "created": "2017-03-20", "title": "Hydrothermal treatment followed by enzymatic hydrolysis and hydrothermal carbonization as means to valorise agro- and forest-based biomass residues", "description": "The suitability of several abundant but underutilized agro and forest based biomass residues for hydrothermal treatment followed by enzymatic hydrolysis as well as for hydrothermal carbonization was studied. The selected approaches represent simple biotechnical and thermochemical treatment routes suitable for wet biomass. Based on the results, the hydrothermal pre-treatment followed by enzymatic hydrolysis seemed to be most suitable for processing of carbohydrate rich corn leaves, corn stover, wheat straw and willow. High content of thermally stable components (i.e. lignin) and low content of ash in the biomass were advantageous for hydrothermal carbonization of grape pomace, coffee cake, Scots pine bark and willow.", "keywords": ["2. Zero hunger", "biomass", "Hydrolysis", "ta220", "0211 other engineering and technologies", "02 engineering and technology", "Forests", "15. Life on land", "enzyme hydrolysis", "Lignin", "Zea mays", "01 natural sciences", "7. Clean energy", "hydrothermal carbonization", "hydrothermal treatment", "lignocellulose", "13. Climate action", "ta219", "SDG 7 - Affordable and Clean Energy", "Biomass", "ta218", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2017.03.095"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2017.03.095", "name": "item", "description": "10.1016/j.biortech.2017.03.095", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2017.03.095"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-01T00:00:00Z"}}, {"id": "10.1016/j.catena.2016.02.013", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:34Z", "type": "Journal Article", "created": "2016-02-27", "title": "Land Use Affects Soil Biochemical Properties In Mt. Kilimanjaro Region", "description": "\u00a9 2016 Elsevier B.V.Microbial parameters have been used to monitor changes in soil quality. Soils from four land use systems common in East Africa and present in the Mt. Kilimanjaro region: (1) montane forest, (2) savannah (3) maize fields and (4) Chagga homegardens were used in laboratory incubations to assess the effects of landuse changes on soil quality. Soil organic matter mineralization and the following microbial parameters: microbial biomass C, mineralization quotient, metabolic quotient and activities of four enzymes: \u03b2-glucosidase, cellobiohydrolase, phosphatase and chitinase were determined. Microbial biomass C content, \u03b2-glucosidase, cellobiohydrolase and chitinase activities were higher in natural systems compared to agricultural soils. High phosphatase activity observed in all land use types reflected strong phosphorus limitation in andic soils of the Mt. Kilimanjaro region. Chitinase activity in montane forest soils was 3 times higher than in Chagga homegardens. Mineralization quotient and cellobiohydrolase activity best exhibited the effect of land-use changes on soil quality in the Mt. Kilimanjaro region. Cellobiohydrolase activity was up to 3 times higher under natural ecosystems compared to agroecosystems. A high percentage of microbial biomass C content in total organic C and low metabolic quotient were observed in Chagga homegarden soils. Soil enzymes (especially cellobiohydrolase) best distinguished between natural and agricultural ecosystems, and are therefore useful for monitoring changes in soil quality. In conclusion, the measured microbial parameters clearly show that the microbial organisms in traditional Chagga homegardens system have high substrate use efficiency. This demonstrates that traditional agroforestry systems promotes soil fertility and are more suitable for agricultural production in the tropics compared to monocropping systems like maize plantations.", "keywords": ["Mineralization quotient", "2. Zero hunger", "Metabolic quotient", "Microbial biomass content", "13. Climate action", "Enzyme activity", "Agroforestry", "Chagga homegardens", "15. Life on land", "630"]}, "links": [{"href": "https://doi.org/10.1016/j.catena.2016.02.013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/CATENA", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.catena.2016.02.013", "name": "item", "description": "10.1016/j.catena.2016.02.013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.catena.2016.02.013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-06-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2012.04.043", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:36Z", "type": "Journal Article", "created": "2012-05-13", "title": "Effectiveness Of Urease Inhibition On The Abatement Of Ammonia, Nitrous Oxide And Nitric Oxide Emissions In A Non-Irrigated Mediterranean Barley Field", "description": "Urea is considered the cheapest and most commonly used form of inorganic N fertilizer worldwide. However, its use is associated with emissions of ammonia (NH(3)), nitrous oxide (N(2)O) and nitric oxide (NO), which have both economic and environmental impact. Urease activity inhibitors have been proposed as a means to reduce NH(3) emissions, although limited information exists about their effect on N(2)O and NO emissions. In this context, a field experiment was carried out with a barley crop (Hordeum vulgare L.) under Mediterranean conditions to test the effectiveness of the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) on reducing these gaseous N losses from surface applied urea. Crop yield, soil mineral N concentrations, dissolved organic carbon (DOC), denitrification potential, NH(3), N(2)O and NO fluxes were measured during the growing season. The inclusion of the inhibitor reduced NH(3) emissions in the 30 d following urea application by 58% and net N(2)O and NO emissions in the 95 d following urea application by 86% and 88%, respectively. NBPT addition also increased grain yield by 5% and N uptake by 6%, although neither increase was statistically significant. Under the experimental conditions presented here, these results demonstrate the potential of the urease inhibitor NBPT in abating NH(3), N(2)O and NO emissions from arable soils fertilized with urea, slowing urea hydrolysis and releasing lower concentrations of NH(4)(+) to the upper soil layer.", "keywords": ["2. Zero hunger", "Nitrous Oxide", "Hordeum", "04 agricultural and veterinary sciences", "Nitric Oxide", "Urease", "6. Clean water", "Organophosphorus Compounds", "Ammonia", "Air Pollution", "Urea", "0401 agriculture", " forestry", " and fisheries", "Enzyme Inhibitors", "Fertilizers"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2012.04.043"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2012.04.043", "name": "item", "description": "10.1016/j.chemosphere.2012.04.043", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2012.04.043"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-09-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2015.06.044", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:37Z", "type": "Journal Article", "created": "2015-07-02", "title": "Contrasting Effects Of Biochar Versus Manure On Soil Microbial Communities And Enzyme Activities In An Aridisol", "description": "Biochar can increase microbial activity, alter microbial community structure, and increase soil fertility in arid and semi-arid soils, but at relatively high rates that may be impractical for large-scale field studies. This contrasts with organic amendments such as manure, which can be abundant and inexpensive if locally available, and thus can be applied to fields at greater rates than biochar. In a field study comparing biochar and manure, a fast pyrolysis hardwood biochar (22.4 Mg ha(-1)), dairy manure (42 Mg ha(-1) dry wt), a combination of biochar and manure at the aforementioned rates, or no amendment (control) was applied to an Aridisol (n=3) in fall 2008. Plots were annually cropped to corn (Zea maize L.). Surface soils (0-30 cm) were sampled directly under corn plants in late June 2009 and early August 2012, and assayed for microbial community fatty acid methyl ester (FAME) profiles and six extracellular enzyme activities involved in soil C, N, and P cycling. Arbuscular mycorrhizal (AM) fungal colonization was assayed in corn roots in 2012. Biochar had no effect on microbial biomass, community structure, extracellular enzyme activities, or AM fungi root colonization of corn. In the short-term, manure amendment increased microbial biomass, altered microbial community structure, and significantly reduced the relative concentration of the AM fungal biomass in soil. Manure also reduced the percent root colonization of corn by AM fungi in the longer-term. Thus, biochar and manure had contrasting short-term effects on soil microbial communities, perhaps because of the relatively low application rate of biochar.", "keywords": ["2. Zero hunger", "04 agricultural and veterinary sciences", "15. Life on land", "Zea mays", "6. Clean water", "Enzymes", "Manure", "Chemistry", "Soil", "Fertility", "Charcoal", "Mycorrhizae", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Extracellular Space", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2015.06.044"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2015.06.044", "name": "item", "description": "10.1016/j.chemosphere.2015.06.044", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2015.06.044"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-01T00:00:00Z"}}, {"id": "10.1016/j.egypro.2011.03.006", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:43Z", "type": "Journal Article", "created": "2011-05-03", "title": "Effects Of Conservation Tillage On Organic Carbon, Nitrogen And Enzyme Activities In A Hydragric Anthrosol Of Chongqing, China", "description": "AbstractPurple paddy (Hydragric Anthrosol in FAO soil classification) is one of important soil resources in Chongqing, China. Long-term conservation tillage may alter distribution of soil organic carbon, nitrogen, and enzyme activities. The objectives of this study were to investigate the impacts of different tillage systems (conventional tillage with rotation of rice and winter fallow (CT-r) system, no-till and ridge culture with rotation of rice and winter fallow (NT-r) system, no-till and ridge culture with rotation of rice and rape (NT-rr) system and conventional tillage with rotation of rice and rape (CT-rr) system) on the depth distribution of soil total organic carbon, nitrogen and enzyme activities (catalase, intverase, and urease activity) in a purple paddy soil after 18 years. Soil total organic carbon and labile organic carbon were significant increased in surface soil layer (0-10cm) under CT-r, NT-r, and NT-rr systems compared to that under CT-rr system. It indicated that conservation tillage practices can sequester soil organic carbon and reduced CO2/CH4 emission. Soil total nitrogen also significant increased in surface soil layer (0-10cm) under CT-r, NT-r, and NT-rr systems with the greatest under CT-r system (36%), followed by under NT-rr system (34%), and the least under NT-r system (20%) compared to CT-rr system. No-till, ridge culture, and rotation of rice and winter fallow were increased soil catalase and urease activities, but the greatest was not observed under NT-r system, under which the catalase activities was significant decreased. Soil invertase activities were significant increased under CT-r system compared to CT-rr systems and only a little increased in 0-20cm soil layer under NT-rr system. Conservation tillage could construct good soil biochemistry environment and maintain soil fertility, and promote agroecosystem sustainable development.", "keywords": ["2. Zero hunger", "Soil organic carbon", "Nitrogen", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "Energy(all)", "Labile organic carbon", "Soil enzyme", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Conservation tillage", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Wang Zi-fang, Luo Youjin, Wei Chaofu, Gao Ming,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.egypro.2011.03.006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Energy%20Procedia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.egypro.2011.03.006", "name": "item", "description": "10.1016/j.egypro.2011.03.006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.egypro.2011.03.006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-01-01T00:00:00Z"}}, {"id": "10.1016/j.ejsobi.2008.09.011", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:15:45Z", "type": "Journal Article", "created": "2008-10-15", "title": "Experimental Drought Reduced Acid And Alkaline Phosphatase Activity And Increased Organic Extractable P In Soil In A Quercus Ilex Mediterranean Forest", "description": "Open AccessPeer reviewed", "keywords": ["NUTRIENT CONTENT", "Quercusilex", "POSTFIRE REGENERATION", "Total soil-P", "Soil", "OAK FOREST", "Litter", "PINUS-HALEPENSIS", "ENZYME-ACTIVITIES", "2. Zero hunger", "Soil organic matter", "Drought", "NE SPAIN", "MICROBIAL BIOMASS", "Leaf P concentration", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Quercus ilex", "PHOSPHORUS LIMITATION", "PLANT-GROWTH", "13. Climate action", "SHORT-TERM", "Alkaline phosphatase activity", "0401 agriculture", " forestry", " and fisheries", "Acid phosphatase activity", "Soil moisture", "Short-term available-P", "Soilorganic matter"]}, "links": [{"href": "https://doi.org/10.1016/j.ejsobi.2008.09.011"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ejsobi.2008.09.011", "name": "item", "description": "10.1016/j.ejsobi.2008.09.011", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ejsobi.2008.09.011"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-09-01T00:00:00Z"}}, {"id": "10.1016/j.ejsobi.2017.10.009", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-07-26T16:15:47Z", "type": "Journal Article", "created": "2017-11-09", "title": "Influence Of A Tropical Grass ( Brachiaria Brizantha Cv. Mulato ) As Cover Crop On Soil Biochemical Properties In A Degraded Agricultural Soil", "description": "Fil: Perez Brandan, Carolina Gabriela. Instituto Nacional de Tecnologia Agropecuaria; Argentina", "keywords": ["2. Zero hunger", "SUSTAINABILITY", "SOIL FUNCTIONALITY", "https://purl.org/becyt/ford/1.6", "MONOCULTURE", "MACRONUTRIENTS", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "https://purl.org/becyt/ford/1", "ENZYMES", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1016/j.ejsobi.2017.10.009"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ejsobi.2017.10.009", "name": "item", "description": "10.1016/j.ejsobi.2017.10.009", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ejsobi.2017.10.009"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-11-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2007.01313.x", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:18:42Z", "type": "Journal Article", "created": "2007-01-19", "title": "Plant Species Richness, Elevated Co2, And Atmospheric Nitrogen Deposition Alter Soil Microbial Community Composition And Function", "description": "AbstractWe determined soil microbial community composition and function in a field experiment in which plant communities of increasing species richness were exposed to factorial elevated CO2 and nitrogen (N) deposition treatments. Because elevated CO2 and N deposition increased plant productivity to a greater extent in more diverse plant assemblages, it is plausible that heterotrophic microbial communities would experience greater substrate availability, potentially increasing microbial activity, and accelerating soil carbon (C) and N cycling. We, therefore, hypothesized that the response of microbial communities to elevated CO2 and N deposition is contingent on the species richness of plant communities. Microbial community composition was determined by phospholipid fatty acid analysis, and function was measured using the activity of key extracellular enzymes involved in litter decomposition. Higher plant species richness, as a main effect, fostered greater microbial biomass, cellulolytic and chitinolytic capacity, as well as the abundance of saprophytic and arbuscular mycorrhizal (AM) fungi. Moreover, the effect of plant species richness on microbial communities was significantly modified by elevated CO2 and N deposition. For instance, microbial biomass and fungal abundance increased with greater species richness, but only under combinations of elevated CO2 and ambient N, or ambient CO2 and N deposition. Cellobiohydrolase activity increased with higher plant species richness, and this trend was amplified by elevated CO2. In most cases, the effect of plant species richness remained significant even after accounting for the influence of plant biomass. Taken together, our results demonstrate that plant species richness can directly regulate microbial activity and community composition, and that plant species richness is a significant determinant of microbial response to elevated CO2 and N deposition. The strong positive effect of plant species richness on cellulolytic capacity and microbial biomass indicate that the rates of soil C cycling may decline with decreasing plant species richness.
", "keywords": ["Extracellular Enzymes", "Complementary Resource Use", "Science", "Ecology and Evolutionary Biology", "Grassland Ecosystem", "Phospholipid Fatty Acid (PLFA)", "Global Change", "14. Life underwater", "complimentary resource use", "global change", "580", "2. Zero hunger", "Plant Diversity", "microbial biomass", "Geology and Earth Sciences", "grasslands", "Soil Fungi", "extracellular enzymes", "04 agricultural and veterinary sciences", "15. Life on land", "Microbial Biomass", "Soil C Cycling", "plant diversity", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "FACE (Free-air Carbon Dioxide Enrichment)"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2007.01313.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2007.01313.x", "name": "item", "description": "10.1111/j.1365-2486.2007.01313.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2007.01313.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-01-19T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2009.04.088", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:16:21Z", "type": "Journal Article", "created": "2009-05-05", "title": "Metal Availability In Heavy Metal-Contaminated Open Burning And Open Detonation Soil: Assessment Using Soil Enzymes, Earthworms, And Chemical Extractions", "description": "The effects of heavy metal contamination on soil enzyme activity and earthworm health (bioaccumulation and condition) were studied in contaminated soils collected from an formerly open burning and open detonation (OBOD) site. Soil extraction methods were also evaluated using CaCl(2) and DTPA solutions as surrogate measures of metal bioavailability and ecotoxicity. Total heavy metal content of the soils ranged from 0.45 to 9.68 mg Cd kg(-1), 8.96 to 5103 mg Cu kg(-1), 40.21 to 328 mg Pb kg(-1), and 56.61 to 10,890 mg Zn kg(-1). Elevated metal concentrations are assumed to be primarily responsible for the reduction in enzyme activities and earthworm health indices. We found significant negative relationships between CaCl(2)- and DTPA-extractable metal content (Cd, Cu, and Zn) and soil enzyme activity (P<0.01). Therefore, it could be concluded that soil enzyme activity and metal bioaccumulation by earthworms can be used as an ecological indicator of metal availability. Furthermore, CaCl(2) and DTPA extraction methods are proved as promising, precise, and inexpensive surrogate measures of Cd, Cu, Pb, and Zn bioavailability from heavy metal-contaminated soils.", "keywords": ["Incineration", "04 agricultural and veterinary sciences", "Pentetic Acid", "01 natural sciences", "6. Clean water", "Enzymes", "Calcium Chloride", "Soil", "13. Climate action", "Metals", " Heavy", "Animals", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Oligochaeta", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2009.04.088"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2009.04.088", "name": "item", "description": "10.1016/j.jhazmat.2009.04.088", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2009.04.088"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-10-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2014.06.074", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-26T16:16:22Z", "type": "Journal Article", "created": "2014-07-14", "title": "Molecular Characterization Of Biochars And Their Influence On Microbiological Properties Of Soil", "description": "The tentative connection between the biochar surface chemical properties and their influence on microbially mediated mineralization of C, N, and S with the help of enzymes is not well established. This study was designed to investigate the effect of different biomass conversion processes (microwave pyrolysis, carbon optimized gasification, and fast pyrolysis using electricity) on the composition and surface chemistry of biochar materials produced from corn stover (Zea mays L.), switchgrass (Panicum virgatum L.), and Ponderosa pine wood residue (Pinus ponderosa Lawson and C. Lawson) and determine the effect of biochars on mineralization of C, N, and S and associated soil enzymatic activities including esterase (fluorescein diacetate hydrolase, FDA), dehydrogenase (DHA), \u03b2-glucosidase (GLU), protease (PROT), and aryl sulfatase (ARSUL) in two different soils collected from footslope (Brookings) and crest (Maddock) positions of a landscape. Chemical properties of biochar materials produced from different batches of gasification process were fairly consistent. Biochar materials were found to be highly hydrophobic (low H/C values) with high aromaticity, irrespective of biomass feedstock and pyrolytic process. The short term incubation study showed that biochar had negative effects on microbial activity (FDA and DHA) and some enzymes including \u03b2-glucosidase and protease.", "keywords": ["2. Zero hunger", "Nitrogen", "04 agricultural and veterinary sciences", "15. Life on land", "Panicum", "Pinus", "Zea mays", "01 natural sciences", "Carbon", "6. Clean water", "Enzymes", "13. Climate action", "Charcoal", "Microscopy", " Electron", " Scanning", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology", "Sulfur", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2014.06.074"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2014.06.074", "name": "item", "description": "10.1016/j.jhazmat.2014.06.074", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2014.06.074"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-08-01T00:00:00Z"}}, {"id": "10.1016/j.jaridenv.2016.01.010", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:16:18Z", "type": "Journal Article", "created": "2016-02-27", "title": "Soil Microbial Functional Capacity And Diversity In A Millet-Shrub Intercropping System Of Semi-Arid Senegal", "description": "Abstract A few species of shrubs grow with dryland row crops in farmers\u2019 fields throughout the Sahel and can significantly increase crop yield. The presence of shrub roots and litter inputs should have implications for soil nutrient pool sizes but there is limited information on the interactions of these shrubs with microbial communities involved in biogeochemical processes. Therefore, the objective of this study was to determine the microbial composition and functional capacity of soil from the rooting zone of pearl millet (Pennisetum glaucum) grown in the presence or absence of the shrub Piliostigma reticulatum in Senegal. Soil samples were collected from a long-term field study where millet was cultivated alone or intercropped with P.\u00a0reticulatum with annual incorporation of coppiced shrub residues. Higher nutrient contents and distinct differences in microbial communities (DGGE profiles) were found between soils from beneath the canopy compared to soil outside the influence of shrubs. The catabolic response profile (MicroResp\u2122) showed that the soil microbial community at both shrub and non-shrub sampling locations, metabolized a wide range of substrates. Trehalose that can work as a signaling molecule was more rapidly degraded in the rooting zone of millet growing in the presence of P.\u00a0reticulatum over millet\u00a0alone. Urease, arylsulfatase and dehydrogenase activities in the millet root zone soil were higher when intercropped with P.\u00a0reticulatum which indicates enhanced potential of biogeochemical processes to proceed in the presence of this shrub. It is concluded that the native shrub P.\u00a0reticulatum promotes a more diverse and active microbial community in the rooting zone of millet and further indicates greater potential to perform decomposition and mineralize nutrients.", "keywords": ["[SDV.SA]Life Sciences [q-bio]/Agricultural sciences", "580", "2. Zero hunger", "[SDV.SA] Life Sciences [q-bio]/Agricultural sciences", "Sub-Saharan Africa", "16S- and ITS-DGGE profiling", "04 agricultural and veterinary sciences", "MicroResp\u2122", "15. Life on land", "Piliostigma reticulatum", "630", "6. Clean water", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "MicroResp (TM)", "16S-and ITS-DGGE profiling", "Pennisetum glaucum (L.) R. Br", "0401 agriculture", " forestry", " and fisheries", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "Pennisetum glaucum (L.) R. Br.", "Soil enzyme activity"]}, "links": [{"href": "https://doi.org/10.1016/j.jaridenv.2016.01.010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Arid%20Environments", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jaridenv.2016.01.010", "name": "item", "description": "10.1016/j.jaridenv.2016.01.010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jaridenv.2016.01.010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-06-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2020.123208", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:16:22Z", "type": "Journal Article", "created": "2020-06-17", "title": "Effects of prescription antibiotics on soil- and root-associated microbiomes and resistomes in an agricultural context", "description": "The use of treated wastewater for crop irrigation is rapidly increasing to respond to the ever-growing demands for water and food resources. However, this practice may contribute to the spread of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) in agricultural settings. To evaluate this potential risk, we analyzed microbiomes and resistomes of soil and Lactuca sativa L. (lettuce) root samples from pots irrigated with tap water spiked with 0, 20, or 100 \u03bcg L-1 of a mixture of three antibiotics (Trimethoprim, Ofloxacin, Sulfamethoxazole). The presence of antibiotics induced changes in bacterial populations, particularly in soil, as revealed by 16S rDNA sequence analysis. Parallel shotgun sequencing identified a total of 56 different ARGs conferring resistance against 14 antibiotic families. Antibiotic -treated samples showed increased loads of ARGs implicated in mutidrug resistance or in both direct and indirect acquired resistance. These changes correlated with the prevalence of Xantomonadales species in the root microbiomes. We interpret these data as indicating different strategies of soil and root microbiomes to cope with the presence of antibiotics, and as a warning that their presence may increase the loads of ARBs and ARGs in edible plant parts, therefore constituting a potential risk for human consumers.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Microbiota", "Angiotensin-Converting Enzyme Inhibitors", "Drug Resistance", " Microbial", "6. Clean water", "Anti-Bacterial Agents", "Angiotensin Receptor Antagonists", "Soil", "03 medical and health sciences", "Prescriptions", "Genes", " Bacterial", "Humans", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2020.123208"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2020.123208", "name": "item", "description": "10.1016/j.jhazmat.2020.123208", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2020.123208"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=enzyme&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=enzyme&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=enzyme&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=enzyme&offset=50", "hreflang": "en-US"}], "numberMatched": 197, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-07-26T16:56:53.568864Z"}