{"type": "FeatureCollection", "features": [{"id": "11585/996230", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:05:35Z", "type": "Journal Article", "created": "2023-10-10", "title": "Beyond PLFA: Concurrent extraction of neutral and glycolipid fatty acids provides new insights into soil microbial communities", "description": "The analysis of phospholipid fatty acids (PLFAs) is one of the most common methods used to quantify the abundance, and analyse the community structure, of soil microbes. The PLFA extraction method can yield two additional lipid fractions\u2014neutral lipids and glycolipids\u2014which potentially hold additional, valuable information on soil microbial communities. Yet its quantitative sensitivity on complete neutral lipid (NLFA) and glycolipid fatty acid (GLFA) profiles has never been validated. In this study we tested (i) if the high-throughput PLFA method can be expanded to concurrently extract complete NLFA and GLFA profiles, as well as sterols, (ii) whether taxonomic specificities of signature fatty acids are retained across the three lipid fractions in pure culture strains, and (iii) whether NLFAs and GLFAs allow soil-specific fingerprinting to the same extent as PLFA analysis. By adjusting the polarity of chloroform with 2% ethanol for solid phase extraction, pure lipid standards were fully fractionated into neutral lipids, glycolipids, and phospholipids. Sterols eluted in the neutral lipid fraction, and a betaine lipid co-eluted with phospholipids. We found consistent taxonomic specificities of fatty acid markers across the three lipid fractions by analysing pure culture extracts representative of soil microbes. Fatty acid profiles from soil extracts, however, showed stronger differences between PLFAs, NLFAs, and GLFAs than between soil types. This indicates that PLFAs and NLFAs signify different community properties (biomass vs. carbon storage, putatively), and that GLFAs are sensitive markers for community traits which behave differently than PLFAs. Although we consistently found high abundances of characteristic sterols in fungal extracts, the PLFA extraction method only yielded miniscule amounts of ergosterol from soil extracts. We argue that concomitant measurement of fatty acid profiles from all three lipid fractions is a low-effort and potentially information-rich addition to the PLFA method, and discuss its applicability for soil microbial community analyses.", "keywords": ["0301 basic medicine", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "15. Life on land", "Soil lipids", "03 medical and health sciences", "106026 \u00d6kosystemforschung", "NLFA", "Ergosterol", "Ergosterol; GLFA; NLFA; Phospholipid fatty acids; Soil lipids", "Phospholipid fatty acid", "soil lipids", "Phospholipid fatty acids", "106022 Microbiology", "GLFA", "106026 Ecosystem research"]}, "links": [{"href": "https://doi.org/11585/996230"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11585/996230", "name": "item", "description": "11585/996230", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11585/996230"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1007/bf00335811", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:55:17Z", "type": "Journal Article", "created": "2002-08-25", "title": "Prolonged Simulated Acid Rain Treatment In The Subarctic: Effect On The Soil Respiration Rate And Microbial Biomass", "description": "Humus chemistry and respiration rate, ATP, ergosterol, and muramic acid concentration as measures of chemical properties, microbial activity, biomass, and indicators of fungal and bacterial biomass were studied in a long-term acid rain experiment in the far north of Finnish Lapland. The treatments used in this study were dry control, irrigated control (spring water, pH 6), and two levels of simulated acid rain (pH 4 and pH 3). Originally (1985\u20131988), simulated acid rain was prepared by adding both H2SO4 and HNO3 (1.9:1 by weight). In 1989 the treatments were modified as follows. In subarea 1 the treatments continued unchanged (H2SO4+HNO3 in rain to pH 4 and pH 3), but in subarea 2 only H2SO4 was applied. The plots were sampled in 1992. The acid application affected humus chemistry by lowering the pH, cation exchange capacity, and base saturation (due to a decrease in Ca and Mg) in the treatment with H2SO4+HNO3 to pH 4 (total proton load over 8 years 2.92 kmol ha-1), whereas the microbial variables were not affected at this proton load, and only the respiration rate decreased by 20% in the strongest simulated acid rain treatment (total proton load 14.9 kmol ha-1). The different ratios of H2SO4+HNO3 in subareas 1 and 2 did not affect the results.", "keywords": ["havumets\u00e4t", "570", "ergosteroli", "0401 agriculture", " forestry", " and fisheries", "hapan sade", "mikrobibiomassa", "04 agricultural and veterinary sciences", "humus", "maan hengitys", "01 natural sciences", "6. Clean water", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Vanhala, P., Fritze, H., Neuvonen, S.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/bf00335811"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/bf00335811", "name": "item", "description": "10.1007/bf00335811", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/bf00335811"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1996-07-01T00:00:00Z"}}, {"id": "10.1007/bf00418673", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:55:18Z", "type": "Journal Article", "created": "2004-11-09", "title": "Wood-Ash Fertilization And Fire Treatments In A Scots Pine Forest Stand: Effects On The Organic Layer, Microbial Biomass, And Microbial Activity", "description": "We studied the reactions of humus layer (F/H) microbial respiratory activity, microbial biomass C, and the fungal biomass, measured as the soil ergosterol content, to the application of three levels of wood ash (1000, 2500, and 5000 kg ha-1) and to fire treatment in a Scots pine (Pinus sylvestris L.) stand. Physicochemical measurements (pH, organic matter content, extractable and total C content, NH                   4                   +                 and total N content, cation-exchange capacity, base saturation) showed similarity between the fire-treated plots and those treated with the lowest dose of wood ash (1000 kg ha-1). The ash application did not change the level of microbial biomass C or fungal ergosterol when compared to the control, being around 7500 and 350 \u03bcg g-1 organic matter for the biomass C and ergosterol, respectively. The fire treatment lowered the values of both biomass measurements to about half that of the control values. The fire treatment caused a sevenfold fall in the respiration rate of fieldmoist soil to 1.8 \u03bcl h-1 g-1 organic matter compared to the values of the control or ash treatments. However, in the same soils adjusted to a water-holding capacity of 60%, the differences between the fire treatment and the control were diminished, and the ash-fertilized plots were characterized by a higher respiration rate compared to the control plots. The glucose-induced respiration reacted in the same way as the water-adjusted soil respiration. The metabolic quotient, qCO2, gradually increased from the control level with increasing applications of ash, reaching a maximum in the fire treatment. Nitrification was not observed in the treatment plots.", "keywords": ["maaper\u00e4n respiraatio", "580", "havumets\u00e4t", "570", "Pinus sylvestris", "nitrifikaatio", "04 agricultural and veterinary sciences", "humus", "15. Life on land", "6. Clean water", "ergosteroli", "metabolia", "kasvualustan indusoima respira", "0401 agriculture", " forestry", " and fisheries"], "contacts": [{"organization": "Fritze, H., Smolander, A., Levula, T., Kitunen, V., M\u00e4lk\u00f6nen, E.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/bf00418673"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/bf00418673", "name": "item", "description": "10.1007/bf00418673", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/bf00418673"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1994-01-01T00:00:00Z"}}, {"id": "10.1007/s00248-007-9308-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:55:21Z", "type": "Journal Article", "created": "2007-09-01", "title": "Long-Term Effect Of Municipal Solid Waste Amendment On Microbial Abundance And Humus-Associated Enzyme Activities Under Semiarid Conditions", "description": "Microbial ecology is the key to understanding the function of soil biota for organic matter cycling after a single amendment of organic waste in semiarid soils. Therefore, in this paper, the long-term effect (17 years) of adding different doses of a solid municipal waste to an arid soil on humus-enzyme complexes, a very stable and long-lasting fraction of soil enzymes, as well as on microbial and plant abundance, was studied. Humic substances were extracted by 0.1 M pH 7 sodium pyrophosphate from soil samples collected in experimental plots amended with different doses of a solid municipal waste (0, 65, 130, 195, and 260 t/ha) 17 years before. The activity of different hydrolases related with the C (beta-glucosidase), N (urease), and P (alkaline phosphatase) cycles and with the formation of humic substances (o-diphenol oxidase) were determined in this extract. The density and diversity of plant cover in the plots, as well as the fungal and bacterial biomass (by analyzing phopholipid fatty acids) were also determined. In general, the amended plots showed greater humic substance-related enzymatic activity than the unamended plots. This activity increased with the dose but only up to a certain level, above which it leveled off or even diminished. Plant diversity and cover density followed the same trend. Fungal and bacterial biomass also benefited in a dose-dependent manner. Different signature molecules representing gram+ and gram- bacteria, and those corresponding to monounsaturated and saturated fatty acids showed a similar behavior. The results demonstrate that organic amendment had a noticeable long-term effect on the vegetal development, humic substances-related enzyme activity and on the development of bacteria and fungi in semiarid conditions.", "keywords": ["2. Zero hunger", "Analysis of Variance", "Time Factors", "Bacteria", "Fatty Acids", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "6. Clean water", "Enzymes", "Refuse Disposal", "12. Responsible consumption", "Soil", "Ergosterol", "11. Sustainability", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Humic Substances", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9308-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9308-0", "name": "item", "description": "10.1007/s00248-007-9308-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9308-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-09-02T00:00:00Z"}}, {"id": "10.1007/s00442-005-0191-4", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:55:30Z", "type": "Journal Article", "created": "2005-07-22", "title": "Fine Root Chemistry And Decomposition In Model Communities Of North-Temperate Tree Species Show Little Response To Elevated Atmospheric Co2 And Varying Soil Resource Availability", "description": "Rising atmospheric [CO2] has the potential to alter soil carbon (C) cycling by increasing the content of recalcitrant constituents in plant litter, thereby decreasing rates of decomposition. Because fine root turnover constitutes a large fraction of annual NPP, changes in fine root decomposition are especially important. These responses will likely be affected by soil resource availability and the life history characteristics of the dominant tree species. We evaluated the effects of elevated atmospheric [CO2] and soil resource availability on the production and chemistry, mycorrhizal colonization, and decomposition of fine roots in an early- and late-successional tree species that are economically and ecologically important in north temperate forests. Open-top chambers were used to expose young trembling aspen (Populus tremuloides) and sugar maple (Acer saccharum) trees to ambient (36 Pa) and elevated (56 Pa) atmospheric CO2. Soil resource availability was composed of two treatments that bracketed the range found in the Upper Lake States, USA. After 2.5 years of growth, sugar maple had greater fine root standing crop due to relatively greater allocation to fine roots (30% of total root biomass) relative to aspen (7% total root biomass). Relative to the low soil resources treatment, aspen fine root biomass increased 76% with increased soil resource availability, but only under elevated [CO2]. Sugar maple fine root biomass increased 26% with increased soil resource availability (relative to the low soil resources treatment), and showed little response to elevated [CO2]. Concentrations of N and soluble phenolics, and C/N ratio in roots were similar for the two species, but aspen had slightly higher lignin and lower condensed tannins contents compared to sugar maple. As predicted by source-sink models of carbon allocation, pooled constituents (C/N ratio, soluble phenolics) increased in response to increased relative carbon availability (elevated [CO2]/low soil resource availability), however, biosynthetically distinct compounds (lignin, starch, condensed tannins) did not always respond as predicted. We found that mycorrhizal colonization of fine roots was not strongly affected by atmospheric [CO2] or soil resource availability, as indicated by root ergosterol contents. Overall, absolute changes in root chemical composition in response to increases in C and soil resource availability were small and had no effect on soil fungal biomass or specific rates of fine root decomposition. We conclude that root contributions to soil carbon cycling will mainly be influenced by fine root production and turnover responses to rising atmospheric [CO2], rather than changes in substrate chemistry.", "keywords": ["0106 biological sciences", "Science", "Climate", "Ecology and Evolutionary Biology", "Plant Roots", "01 natural sciences", "Trees", "Sugar Maple", "Soil", "Ergosterol", "Health Sciences", "Carbon-based Secondary Compounds", "Biomass", "Cellular and Developmental Biology", "Ecosystem", "Soil Microbiology", "Atmosphere", "Fungi", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Soil C Cycling", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Trembling Aspen"]}, "links": [{"href": "https://doi.org/10.1007/s00442-005-0191-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-005-0191-4", "name": "item", "description": "10.1007/s00442-005-0191-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-005-0191-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-07-23T00:00:00Z"}}, {"id": "10.1007/s11356-017-0378-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:56:03Z", "type": "Journal Article", "created": "2017-10-09", "title": "Intra- and inter-specific variations in chitin in lichens along a N-deposition gradient", "description": "The mechanisms of nitrogen (N) tolerance in lichens are not yet fully understood. Here, we investigated how the increase of chitin content is related with N excess at inter- and intra-specific levels, by using species with differing ecological N tolerances (the tolerant Xanthoria parietina and Parmotrema hypoleucinum and the sensitive Evernia prunastri and Usnea sp.) and thalli of X. parietina and P. hypoleucinum from sites with different availabilities of N of agricultural origin (livestock), as confirmed by lichen N content and \u03b415N. Nitrogen, chitin (N-containing compound), and ergosterol contents were measured in lichen thalli. Nitrogen and chitin contents were higher in tolerant species than those in sensitive ones (inter-specific level) and in thalli collected from the N-polluted site than in thalli from the clean site (intra-specific level). We suggest that chitin contributes to N stress tolerance in lichens, and that excess N can be partially stored as chitin (non-toxic form) in the cell walls of tolerant species.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Ascomycota", "Biological Variation", " Population", "Lichens", "Nitrogen", "Ergosterol", "Chitin", "15. Life on land"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s11356-017-0378-3.pdf"}, {"href": "https://doi.org/10.1007/s11356-017-0378-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-017-0378-3", "name": "item", "description": "10.1007/s11356-017-0378-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-017-0378-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-10-09T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2006.03.038", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:56:42Z", "type": "Journal Article", "created": "2006-05-09", "title": "Decomposition Of Heavy Metal Contaminated Nettles (Urtica Dioica L.) In Soils Subjected To Heavy Metal Pollution By River Sediments", "description": "Two incubation experiments were conducted to evaluate differences in the microbial use of non-contaminated and heavy metal contaminated nettle (Urtica dioica L.) shoot residues in three soils subjected to heavy metal pollution (Zn, Pb, Cu, and Cd) by river sediments. The microbial use of shoot residues was monitored by changes in microbial biomass C, biomass N, biomass P, ergosterol, N mineralisation, CO(2) production and O(2) consumption rates. Microbial biomass C, N, and P were estimated by fumigation extraction. In the non-amended soils, the mean microbial biomass C to soil organic C ratio decreased from 2.3% in the low metal soil to 1.1% in the high metal soils. In the 42-d incubation experiment, the addition of 2% nettle residues resulted in markedly increased contents of microbial biomass P (+240%), biomass C (+270%), biomass N (+310%), and ergosterol (+360%). The relative increase in the four microbial properties was similar for the three soils and did not show any clear heavy metal effect. The contents of microbial biomass C, N and P and ergosterol contents declined approximately by 30% during the incubation as in the non-amended soils. The ratios microbial biomass C to N, microbial biomass C to P, and ergosterol to microbial biomass C remained constant at 5.2, 26, and 0.5%, respectively. In the 6-d incubation experiment, the respiratory quotient CO(2)/O(2) increased from 0.74 in the low metal soil to 1.58 in the high metal soil in the non-amended soils. In the treatments amended with 4% nettle residues, the respiratory quotient was constant at 1.13, without any effects of the three soils or the two nettle treatments. Contaminated nettle residues led generally to significantly lower N mineralisation, CO(2) production and O(2) consumption rates than non-contaminated nettle residues. However, the absolute differences were small.", "keywords": ["2. Zero hunger", "Geologic Sediments", "Minerals", "Time Factors", "Nitrogen", "Urtica dioica", "04 agricultural and veterinary sciences", "Carbon Dioxide", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "Oxygen", "Rivers", "13. Climate action", "Ergosterol", "Metals", " Heavy", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology", "Environmental Monitoring", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Rainer Georg Joergensen, Khalid Saifullah Khan,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2006.03.038"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2006.03.038", "name": "item", "description": "10.1016/j.chemosphere.2006.03.038", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2006.03.038"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-11-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2023.109205", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:57:51Z", "type": "Journal Article", "created": "2023-10-10", "title": "Beyond PLFA: Concurrent extraction of neutral and glycolipid fatty acids provides new insights into soil microbial communities", "description": "The analysis of phospholipid fatty acids (PLFAs) is one of the most common methods used to quantify the abundance, and analyse the community structure, of soil microbes. The PLFA extraction method can yield two additional lipid fractions\u2014neutral lipids and glycolipids\u2014which potentially hold additional, valuable information on soil microbial communities. Yet its quantitative sensitivity on complete neutral lipid (NLFA) and glycolipid fatty acid (GLFA) profiles has never been validated. In this study we tested (i) if the high-throughput PLFA method can be expanded to concurrently extract complete NLFA and GLFA profiles, as well as sterols, (ii) whether taxonomic specificities of signature fatty acids are retained across the three lipid fractions in pure culture strains, and (iii) whether NLFAs and GLFAs allow soil-specific fingerprinting to the same extent as PLFA analysis. By adjusting the polarity of chloroform with 2% ethanol for solid phase extraction, pure lipid standards were fully fractionated into neutral lipids, glycolipids, and phospholipids. Sterols eluted in the neutral lipid fraction, and a betaine lipid co-eluted with phospholipids. We found consistent taxonomic specificities of fatty acid markers across the three lipid fractions by analysing pure culture extracts representative of soil microbes. Fatty acid profiles from soil extracts, however, showed stronger differences between PLFAs, NLFAs, and GLFAs than between soil types. This indicates that PLFAs and NLFAs signify different community properties (biomass vs. carbon storage, putatively), and that GLFAs are sensitive markers for community traits which behave differently than PLFAs. Although we consistently found high abundances of characteristic sterols in fungal extracts, the PLFA extraction method only yielded miniscule amounts of ergosterol from soil extracts. We argue that concomitant measurement of fatty acid profiles from all three lipid fractions is a low-effort and potentially information-rich addition to the PLFA method, and discuss its applicability for soil microbial community analyses.", "keywords": ["0301 basic medicine", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "15. Life on land", "Soil lipids", "03 medical and health sciences", "106026 \u00d6kosystemforschung", "NLFA", "Ergosterol", "Ergosterol; GLFA; NLFA; Phospholipid fatty acids; Soil lipids", "Phospholipid fatty acid", "soil lipids", "Phospholipid fatty acids", "106022 Microbiology", "GLFA", "106026 Ecosystem research"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2023.109205"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2023.109205", "name": "item", "description": "10.1016/j.soilbio.2023.109205", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2023.109205"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1111/nph.19450", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:59:53Z", "type": "Journal Article", "created": "2023-11-30", "title": "A step forward in fungal biomass estimation \u2013 a new protocol for more precise measurements of soil ergosterol with liquid chromatography\u2010mass spectrometry and comparison of extraction methods", "description": "Significant differences between ergosterol protocols and their yield call for harmonization of methodologies. We propose an extraction protocol with KOH in methanol with cyclohexane for liquid\u2013liquid extraction. The novel LC\u2013MS method proved to be superior to the HPLC-UV method for soil samples because of the higher quality of the peaks and the possibility of following peak purity. Moreover, the LC\u2013MS method has 10 times lower detection limit than that of HPLC-UV.", "keywords": ["Soil", "selected ion recording", "Liquid Chromatography-Mass Spectrometry", "Ergosterol", "mineral soil", "ta1182", "Fungi", "ergosterol fragmentation", "Biomass", "540", "forest soil", "peatlands", "Soil Microbiology"]}, "links": [{"href": "https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.19450"}, {"href": "https://doi.org/10.1111/nph.19450"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.19450", "name": "item", "description": "10.1111/nph.19450", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.19450"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-11-29T00:00:00Z"}}, {"id": "10.5061/dryad.79cnp5htw", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:02:13Z", "type": "Dataset", "title": "Data from: A tipping-point in carbon storage when forest expands into tundra is related to mycorrhizal recycling of nitrogen", "description": "unspecifiedTundra ecosystems are global belowground sinks for atmospheric CO2.  Ongoing warming-induced encroachment by shrubs and trees risks turning  this sink into a CO2 source, resulting in a positive feedback on climate  warming. To advance mechanistic understanding of how shifts in mycorrhizal  types affect long-term carbon (C) and nitrogen (N) stocks, we studied  small-scale soil depth profiles of fungal communities and C-N dynamics  across a subarctic-alpine forest-heath vegetation gradient. Belowground  organic stocks decreased abruptly at the transition from heath to forest,  linked to the presence of certain tree-associateds ectomycorrhizal fungi  that contribute to decomposition when mining N from organic matter. In  contrast, ericoid mycorrhizal plants and fungi were associated with  organic matter accumulation and slow decomposition. If climatic controls  on arctic-alpine forest lines are relaxed, increased decomposition will  likely outbalance increased plant productivity, decreasing the overall C  sink capacity of displaced tundra.", "keywords": ["C-N dynamics", "ectomycorrhizal exploration type", "functional genes", "ergosterol", "ITS2 meta-barcoding", "Fungal community", "Arctic greening", "Climate feedback", "15. Life on land", "litter saprotrophs", "mycorrhizal type", "litter bags", "13. 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Data presented by  litterbag and by plot. Soil properties include: mineral N, potential N  mineralisation, soil organic matter, soil pH, and also concentrations of  PLFA markers and ergosterol. Daily averages of soil temperature and  moisture present for limited number of plots. Names of cover crops  abbreviated as follows: Lolium perenne (Lope), Trifolium repense (Trre),  Raphanus sativus (Rasa), Vicia sativa (Visa). Main crops: Avena sativa  (Avsa), Cichorium endivia (Cien).Barel-JAPPL-2017-01119.R3  data.xlsx", "keywords": ["2. Zero hunger", "decomposition", "ergosterol", "Lolium perenne", "Vicia sativa", "Verwerkte data", "Raphanus sativus", "Avena sativa", "microbial community composition", "carbon cycling", "Soil pH", "15. Life on land", "mineral nitrogen", "Cichorium endivia", "nitrogen cycling", "crop rotation", "standardised substrates", "13. 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Here, we investigated how the increase of chitin content is related with N excess at inter- and intra-specific levels, by using species with differing ecological N tolerances (the tolerant Xanthoria parietina and Parmotrema hypoleucinum and the sensitive Evernia prunastri and Usnea sp.) and thalli of X. parietina and P. hypoleucinum from sites with different availabilities of N of agricultural origin (livestock), as confirmed by lichen N content and \u03b415N. Nitrogen, chitin (N-containing compound), and ergosterol contents were measured in lichen thalli. Nitrogen and chitin contents were higher in tolerant species than those in sensitive ones (inter-specific level) and in thalli collected from the N-polluted site than in thalli from the clean site (intra-specific level). We suggest that chitin contributes to N stress tolerance in lichens, and that excess N can be partially stored as chitin (non-toxic form) in the cell walls of tolerant species.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Ascomycota", "Biological Variation", " Population", "Lichens", "Nitrogen", "Ergosterol", "Chitin", "15. 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Here, we investigated how the increase of chitin content is related with N excess at inter- and intra-specific levels, by using species with differing ecological N tolerances (the tolerant Xanthoria parietina and Parmotrema hypoleucinum and the sensitive Evernia prunastri and Usnea sp.) and thalli of X. parietina and P. hypoleucinum from sites with different availabilities of N of agricultural origin (livestock), as confirmed by lichen N content and \u03b415N. Nitrogen, chitin (N-containing compound), and ergosterol contents were measured in lichen thalli. Nitrogen and chitin contents were higher in tolerant species than those in sensitive ones (inter-specific level) and in thalli collected from the N-polluted site than in thalli from the clean site (intra-specific level). We suggest that chitin contributes to N stress tolerance in lichens, and that excess N can be partially stored as chitin (non-toxic form) in the cell walls of tolerant species.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Ascomycota", "Biological Variation", " Population", "Lichens", "Nitrogen", "Ergosterol", "Chitin", "15. 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Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of the ZALF Datenerfassung's research activities.\" Although every care has been taken in preparing and testing the data, the ZALF Datenerfassung and the BonaRes Data Centre cannot guarantee that the data are correct; neither does the ZALF Datenerfassung and the BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The ZALF Datenerfassung and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data.", "updated": "2025-03-07", "type": "Dataset", "created": "2024-12-04", "language": "eng", "title": "Can potato cropping be made regenerative? Cover crops and dead organic mulch support soil microbial activity.", "description": "This dataset contains the results of microbial biomass carbon and nitrogen, basal respiration and fungal ergosterol which were measured and analyzed during 2019 and 2023 at the experimental farm of Kassel University in Neu-Eichenberg, Hesse, Germany. Three experiments were conducted to investigate the effects of cover crop type prior to potato cropping and the application of dead organic mulch to potatoes on soil microbial indices. 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