{"type": "FeatureCollection", "features": [{"id": "10.1007/s00374-011-0539-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:14:45Z", "type": "Journal Article", "created": "2011-01-18", "title": "Effects Of Organic And Inorganic Fertilization On Soil Bacterial And Fungal Microbial Diversity In The Kabete Long-Term Trial, Kenya", "description": "The effects of crop manure and inorganic fertilizers on composition of microbial communities of central high land soils of Kenya are poorly known. For this reason, we have carried out a thirty-two-year-old long-term trial in Kabete, Kenya. These soils were treated with organic (maize stover (MS) at 10 t ha\u22121, farmyard manure (FYM) at 10 t ha\u22121) and inorganic fertilizers 120 kg N, 52.8 kg P (N2P2), N2P2 + MS, N2P2 + FYM, a control, and a fallow for over 30 years. We examined 16S rRNA gene and 28S rRNA gene fingerprints of bacterial and fungal diversity by PCR amplification and denaturing gradient gel electrophoresis separation, respectively. The PCR bacterial community structure and diversity were negatively affected by N2P2 and were more closely related to the bacterial structure in the soils without any addition (control) than that of soils with a combination of inorganic and organic or inorganic fertilizers alone. The effect on fungal diversity by N2P2 was different than the effect on bacterial diversity since the fungal diversity was similar to that of the N2P2 + FYM and N2P2 + MS-treated. However, soils treated with organic inputs clustered away from soils amended with inorganic inputs. Organic inputs had a positive effect on both bacterial and fungal diversity with or without chemical fertilizers. Results from this study suggested that total diversity of bacterial and fungal communities was closely related to agro-ecosystem management practices and may partially explain the yield differences observed between the different treatments.", "keywords": ["[SDV.SA]Life Sciences [q-bio]/Agricultural sciences", "Microbial diversity", "soil microorganisms", "engrais organique", "http://aims.fao.org/aos/agrovoc/c_27870", "Organic and inorganic amendments", "F08 - Syst\u00e8mes et modes de culture", "rendement des cultures", "630", "fertilisation", "biodiversit\u00e9", "http://aims.fao.org/aos/agrovoc/c_4592", "http://aims.fao.org/aos/agrovoc/c_36669", "http://aims.fao.org/aos/agrovoc/c_2018", "inorganic fertilizers", "http://aims.fao.org/aos/agrovoc/c_10795", "http://aims.fao.org/aos/agrovoc/c_34326", "fertility", "2. Zero hunger", "[SDV.SA] Life Sciences [q-bio]/Agricultural sciences", "http://aims.fao.org/aos/agrovoc/c_33949", "g\u00e9n\u00e9tique des populations", "04 agricultural and veterinary sciences", "agro\u00e9cosyst\u00e8me", "6. Clean water", "fertilit\u00e9 du sol", "PCR", "http://aims.fao.org/aos/agrovoc/c_34079", "polymerization", "community structure", "abonos inorg\u00e1nicos", "management", "570", "http://aims.fao.org/aos/agrovoc/c_7170", "http://aims.fao.org/aos/agrovoc/c_7172", "flore microbienne", "soil", "http://aims.fao.org/aos/agrovoc/c_36167", "micro-organisme du sol", "http://aims.fao.org/aos/agrovoc/c_10176", "organic fertilizers", "abonos org\u00e1nicos", "pratique culturale", "microorganismos del suelo", "suelo", "flore du sol", "P35 - Fertilit\u00e9 du sol", "P34 - Biologie du sol", "polimerizaci\u00f3n", "15. Life on land", "engrais min\u00e9ral", "http://aims.fao.org/aos/agrovoc/c_16367", "http://aims.fao.org/aos/agrovoc/c_4086", "0401 agriculture", " forestry", " and fisheries", "F04 - Fertilisation"]}, "links": [{"href": "https://doi.org/10.1007/s00374-011-0539-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00374-011-0539-3", "name": "item", "description": "10.1007/s00374-011-0539-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00374-011-0539-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-01-19T00:00:00Z"}}, {"id": "10.1007/s11104-016-2949-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:15:20Z", "type": "Journal Article", "created": "2016-06-14", "title": "Phosphorus Availability And Microbial Community In The Rhizosphere Of Intercropped Cereal And Legume Along A P-Fertilizer Gradient", "description": "Positive below-ground interactions (facilitation) should be more pronounced when resources limit crop growth, according to the stress-gradient hypothesis. Our aim was to test this hypothesis for intercropped durum wheat and faba bean along a P-fertilizer gradient. A field experiment was conducted in a long-term P-fertilizer trial with three rates of P-fertilization (No, Low and High P). Microbial biomass was assessed by chloroform fumigation-extraction. Quantitative PCR was applied to evaluate the abundance of relevant microbial groups. Phosphorus availability and microbial biomass systematically increased in the rhizosphere compared to bulk soil. P-fertilization resulted in higher abundance of targeted bacterial phyla, whole bacterial and fungal communities, and depressed mycorrhizal colonization of durum wheat, but not faba bean. Microbial biomass carbon significantly increased in the rhizosphere only in P-fertilized treatments, pointing to P limitation of microbial communities. Intercropping yielded a significant effect on rhizosphere microbial properties only at High P. Microbial biomass P increased in the rhizosphere of intercropped faba bean only at No P level, and was thus the sole finding supporting the stress-gradient hypothesis. P-fertilization was the main driver of microbial communities in this field trial, and P-fertilizer application modulated the species-specific effect in the intercrop. Plant performance did not validate the stress-gradient hypothesis as positive plant-plant interactions occurred regardless of the level of P-fertilization.", "keywords": ["[SDE] Environmental Sciences", "engrais phosphat\u00e9", "F08 - Syst\u00e8mes et modes de culture", "[SDV]Life Sciences [q-bio]", "F62 - Physiologie v\u00e9g\u00e9tale - Croissance et d\u00e9veloppement", "mycorhization", "Triticum turgidum", "630", "fertilisation", "[SHS]Humanities and Social Sciences", "http://aims.fao.org/aos/agrovoc/c_37554", "http://aims.fao.org/aos/agrovoc/c_5800", "http://aims.fao.org/aos/agrovoc/c_10795", "http://aims.fao.org/aos/agrovoc/c_24199", "2. 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