{"type": "FeatureCollection", "features": [{"id": "10.3389/fmicb.2016.01446", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:23:18Z", "type": "Journal Article", "created": "2016-09-14", "description": "Soil management is fundamental to all agricultural systems and fertilization practices have contributed substantially to the impressive increases in food production. Despite the pivotal role of soil microorganisms in agro-ecosystems, we still have a limited understanding of the complex response of the soil microbiota to organic and mineral fertilization in the very long-term. Here, we report the effects of different fertilization regimes (mineral, organic and combined mineral and organic fertilization), carried out for more than a century, on the structure and activity of the soil microbiome. Organic matter content, nutrient concentrations, and microbial biomass carbon were significantly increased by mineral, and even more strongly by organic fertilization. Pyrosequencing revealed significant differences between the structures of bacterial and fungal soil communities associated to each fertilization regime. Organic fertilization increased bacterial diversity, and stimulated microbial groups (Firmicutes, Proteobacteria, and Zygomycota) that are known to prefer nutrient-rich environments, and that are involved in the degradation of complex organic compounds. In contrast, soils not receiving manure harbored distinct microbial communities enriched in oligotrophic organisms adapted to nutrient-limited environments, as Acidobacteria. The fertilization regime also affected the relative abundances of plant beneficial and detrimental microbial taxa, which may influence productivity and stability of the agroecosystem. As expected, the activity of microbial exoenzymes involved in carbon, nitrogen, and phosphorous mineralization were enhanced by both types of fertilization. However, in contrast to comparable studies, the highest chitinase and phosphatase activities were observed in the solely mineral fertilized soil. Interestingly, these two enzymes showed also a particular high biomass-specific activities and a strong negative relation with soil pH. As many soil parameters are known to change slowly, the particularity of unchanged fertilization treatments since 1902 allows a profound assessment of linkages between management and abiotic as well as biotic soil parameters. Our study revealed that pH and TOC were the majors, while nitrogen and phosphorous pools were minors, drivers for structure and activity of the soil microbial community. Due to the long-term treatments studied, our findings likely represent permanent and stable, rather than transient, responses of soil microbial communities to fertilization.", "keywords": ["Soil nutrients", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "long-term fertilization", "microbial biomass", "15. Life on land", "microbial activity", "Microbiology", "QR1-502", "03 medical and health sciences", "13. Climate action", "soil microbial communities", "soil nutrients", "454 pyrosequencing"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2016.01446"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.01446", "name": "item", "description": "10.3389/fmicb.2016.01446", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.01446"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-14T00:00:00Z"}}, {"id": "10.1007/s00374-010-0455-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:15:33Z", "type": "Journal Article", "created": "2010-03-26", "title": "Reversing Agriculture From Intensive To Sustainable Improves Soil Quality In A Semiarid South Italian Soil", "description": "Intensive agriculture (IA) is widespread in South Italy, although it requires frequent tillage, large amounts of fertilizers and irrigation water. We have assessed the efficacy of reversing IA to sustainable agriculture (SA) in recovering quality of a typical South Italy soil (Lithic Haploxeralf). This reversion, lasting from 2000 to 2007, replaced 75% of nutrients formerly supplied inorganically by farmyard manuring and reduced the tillage frequency. Several chemical and biochemical properties, functionally related to C and N mineralisation\u2013immobilisation processes and to P and S nutrient cycles, were monitored annually from 2005 to 2007 in the spring. Reversing IA to SA decreased soil bulk density, almost doubled the soil organic matter (SOM) as favoured the immobilisation of C and N, increased most soil microbial indicators but decreased contents of nitrate, mineral N and K2SO4-extractable C. The K2SO4-extractable C/K2SO4-extractable organic N ratio suggested that substrate quality rather than the mass of readily available C and N affected biomass and activity of soil microflora. Also, the largely higher 10-day-evolved CO2\u2013C-to-inorganic N ratio under SA than IA indicated that higher C mineralisation, associated with higher microbial biomass N immobilisation, occurred under SA than IA. Decreases in most soil enzyme activities under IA, compared to SA, were much higher than concomitant decreases in SOM content. Soil salinity and sodicity were always higher in IA than SA soil, although not critically high, likely due to the intensive inorganic fertilisation as irrigation waters were qualitatively and quantitatively the same between the two soils. Thus, we suggest that the cumulative small but long-term saline (osmotic) and sodic (dispersing) effects in IA soil decreased the microbial variables more than total organic C and increased soil bulk density.", "keywords": ["2. Zero hunger", "Soil quality; Sustainable agriculture; Soil microbial biomass; Microbial activity; Semiarid Mediterranean environment", "Sustainable agriculture", "Soil microbial biomass", "Soil quality ; Sustainable agriculture .; Soil chemstry", "04 agricultural and veterinary sciences", "15. Life on land", "Soil quality", "6. Clean water", "12. Responsible consumption", "Microbial activity", "Semiarid Mediterranean environment", "13. Climate action", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1007/s00374-010-0455-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00374-010-0455-y", "name": "item", "description": "10.1007/s00374-010-0455-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00374-010-0455-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-03-27T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2007.12.012", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:16:55Z", "type": "Journal Article", "created": "2008-02-11", "title": "Effects Of Living Mulches Or Residue Amendments On Soil Microbial Properties In Direct Seeded Cropping Systems Of Madagascar", "description": "Abstract   There is growing recognition for the need to study the impact of agricultural land uses on biological and biochemical properties of soils. In Madagascar, cropping systems based on direct seeding with permanent vegetation cover provide a new means for sustainable agriculture to protect the environment and make the most of natural resources. This study assessed the effects of different direct seeding mulch-based cropping systems on soil microbial biomass and activities. The soil was andic Dystrustept. Samples of the soil were taken from 0 to 5\u00a0cm soil layer of three direct seeding mulch-based cropping systems (DMC using crop residues and living mulches). The samples were compared with samples from conventionally tilled plots (CT) and natural fallows (NF). The field experiments were carried out over a 12-year-period and two types of amendment were applied once a year at sowing, farmyard manure (FYM) and farmyard manure combined with an NPK chemical fertilizer. The C and N content, microbial basal respiration and biomass and \u03b2-glucosidase, urease and acid phosphatase activities were determined. The results showed that there was no interaction between soil management strategies and the use of fertilizer. Furthermore, the fertilizer did not affect the soil C and N content or the acid phosphatase and urease activities. Farmyard manure with added NPK had a significantly greater effect than farmyard manure on its own, increasing the microbial biomass, soil respiration and \u03b2-glucosidase activity up to 26%, 52% and 20%, respectively but there was no significant difference between natural fallows and direct seeding mulch-based cropping systems. However, conventional tillage showed a significantly lower soil microbial biomass, C content, microbial respiration and urease activity than natural fallows. The results for direct seeding mulch-based systems varied according to the microbial activities measured. However, soil \u03b2-glucosidase and acid phosphatase activities were significantly higher for the direct seeding mulch-based systems using crop residues than for the direct seeding mulch-based systems using living mulches. Direct seeding mulch-based systems with Desmodium uncinatum living mulch had significantly lower microbial biomass and respiration than the other direct seeding mulch-based systems. There was less evidence of change in the soil between natural fallow and direct seeding mulch-based systems but a higher build-up of some microbial properties was obtained for direct seeding mulch-based systems soil than in conventionally tilled soils and natural fallow.", "keywords": ["crop residues", "Glycine max", "http://aims.fao.org/aos/agrovoc/c_7170", "living mulch", "microbial activity", "Zea mays", "630", "http://aims.fao.org/aos/agrovoc/c_36167", "micro-organisme du sol", "http://aims.fao.org/aos/agrovoc/c_33553", "http://aims.fao.org/aos/agrovoc/c_3301", "http://aims.fao.org/aos/agrovoc/c_4510", "http://aims.fao.org/aos/agrovoc/c_16118", "http://aims.fao.org/aos/agrovoc/c_35657", "F07 - Fa\u00e7ons culturales", "2. Zero hunger", "microbial biomass", "http://aims.fao.org/aos/agrovoc/c_2604", "r\u00e9sidu de r\u00e9colte", "activit\u00e9 enzymatique", "P35 - Fertilit\u00e9 du sol", "04 agricultural and veterinary sciences", "http://aims.fao.org/aos/agrovoc/c_25803", "15. Life on land", "andic Dystrustept", "semis direct", "6. Clean water", "fertilit\u00e9 du sol", "http://aims.fao.org/aos/agrovoc/c_8504", "[SDE]Environmental Sciences", "direct seeding", "respiration du sol", "0401 agriculture", " forestry", " and fisheries", "F04 - Fertilisation", "mati\u00e8re organique du sol"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2007.12.012"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2007.12.012", "name": "item", "description": "10.1016/j.apsoil.2007.12.012", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2007.12.012"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-06-01T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2010.04.006", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:16:56Z", "type": "Journal Article", "created": "2010-05-22", "title": "Effect Of The Endogeic Earthworm Pontoscolex Corethrurus On The Microbial Structure And Activity Related To Co2 And N2o Fluxes From A Tropical Soil (Madagascar)", "description": "Abstract   The objective of this laboratory study was to determine the influence of a tropical endogeic earthworm,  Pontoscolex corethrurus , on CO 2  and N 2 O fluxes from a tropical Ferralsol and microorganisms potentially involved in these gases emissions. CO 2  and N 2 O fluxes were measured during 35 days from soil mesocosms with and without earthworms. At the end of the incubation, 7% of soil was egested as cast in the earthworm treatment. Then, casts which may be aged from few hours to 35 days old were isolated from non-ingested soil. Different descriptive parameters (activity, density, and structure) of the microbial communities were investigated in the control, the non-ingested soils, and the casts. Quantitative PCR of denitrification genes encoding the nitrite ( nirK ) and nitrous oxide ( nosZ ) reductases was used to study denitrifier density in the earthworm casts. The presence of  P. corethrurus  induced a significant increase in CO 2  emissions but did not affect N 2 O fluxes when measured at mesocosm level. Despite the absence of significant differences in C and N contents between soils and casts, the near infra-red spectra analysis clearly underlined a specific organic signature for the casts. Fungal and bacterial biomass significantly decreased (\u223c2-fold) in casts compared to parent soil, but the fungal-to-bacterial ratio was not modified by the earthworm casting activity. Data suggested that bacterial communities, especially denitrifiers, were modified in casts. The relative abundance of  nirK  and  nosZ  genes increased in the casts while the genetic structures of total bacteria and denitrifying communities were slightly modified in the casts. This study highlighted the importance of earthworm casts as a specific soil habitat where a subset of soil functional bacterial communities (such as denitrifiers) found favourable condition for their growth. However the effect of  P. corethrurus  was less evident when up-scaling from casts to mesocosm level.", "keywords": ["Pontoscolex corethrurus", "Quantitative PCR", "Denitrification genes", "Madagascar", "0401 agriculture", " forestry", " and fisheries", "Microbial activity potentials", "04 agricultural and veterinary sciences", "01 natural sciences", "630", "FAME", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2010.04.006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2010.04.006", "name": "item", "description": "10.1016/j.apsoil.2010.04.006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2010.04.006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-07-01T00:00:00Z"}}, {"id": "10.1016/j.catena.2016.12.013", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-06-27T16:17:08Z", "type": "Journal Article", "created": "2016-12-29", "title": "Manure Fertilization Increases Soil Respiration And Creates A Negative Carbon Budget In A Mediterranean Maize (Zea Mays L.)-Based Cropping System", "description": "Abstract   Agronomic research is important to identify suitable options for improving soil carbon (C) sequestration and reducing soil CO2 emissions. Therefore, the objectives of this study were i) to analyse the on-farm effects of different nitrogen fertilization sources on soil respiration, ii) to explore the effect of fertilization on soil respiration sensitivity to soil temperature (T) and iii) to assess the effect of the different fertilization regimes on the soil C balance. We hypothesized that i) the soil CO2 emission dynamics in Mediterranean irrigated cropping systems were mainly affected by fertilization management and T and ii) fertilization affected the soil C budget via different C inputs and CO2 efflux. Four fertilization systems (farmyard manure, cattle slurry, cattle slurry\u00a0+\u00a0mineral, and mineral) were compared in a double-crop rotation based on silage maize (Zea mays L.) and a mixture of Italian ryegrass (Lolium multiflorum Lam.) and oats (Avena sativa L.). The research was performed in the dairy district of Arborea, in the coastal zone of Sardinia (Italy), from May 2011 to May 2012. The soil was a Psammentic Palexeralfs with a sandy texture (940\u00a0g\u00a0sand\u00a0kg\u2212\u00a01). The soil total respiration (SR), heterotrophic respiration (Rh), T and soil water content (SWC) were simultaneously measured in situ. The soil C balance was computed considering the Rh C losses and the soil C inputs from fertilizer and crop residues. The results showed that the maximum soil CO2 emission rates soon after the application of organic fertilizer reached values up to 12\u00a0\u03bcmol\u00a0m\u2212\u00a02\u00a0s\u2212\u00a01. On average, the manure fertilizer showed significantly higher CO2 emissions, which resulted in a negative annual C balance (\u2212\u00a02.9\u00a0t\u00a0ha\u2212\u00a01). T also affected the soil respiration temporal dynamics during the summer, consistently with results obtained in other temperate climatic regions that are characterized by wet summers and contrary to results from rainfed Mediterranean systems where the summer SR and Rh are constrained by the low SWC. The sensitivity of soil respiration to temperature significantly increased with C input from fertilizer. In conclusion, this research supported the hypotheses tested. Furthermore, the results indicated that i) soil CO2 efflux was significantly affected by fertilization management and T, and ii) fertilization with manure increased the soil respiration and resulted in a significantly negative soil C budget. This latter finding could be primarily explained by a reduction in productivity and, consequently, in crop residue with organic fertilization alone as compared to mineral, by the favourable SWC and T for mineralization, and by the sandy soil texture, which hindered the formation of macroaggregates and hence soil C stabilization, making fertilizer organic inputs highly susceptible to mineralization.", "keywords": ["2. Zero hunger", "13. Climate action", "Biomass C turnover GHG emission Microbial activity Soil moisture", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.1016/j.catena.2016.12.013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/CATENA", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.catena.2016.12.013", "name": "item", "description": "10.1016/j.catena.2016.12.013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.catena.2016.12.013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-04-01T00:00:00Z"}}, {"id": "10.1016/j.ejsobi.2013.10.007", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-06-27T16:17:21Z", "type": "Journal Article", "created": "2013-11-02", "title": "Effect Of Biochar Addition On Soil Microbial Community In A Wheat Crop", "description": "Biochar is known to enhance soil fertility and C sequestration, but relatively little information is currently available about its effect on soil microbial community, a component of terrestrial ecosystems that plays a key role in nutrient cycling. This study tested the effects of soil amendment with two loads of wood-derived biochar (30 and 60 t ha(-1)) in a wheat crop in Tuscany (Italy). Soil samples were collected 3 and 14 months after treatments over two successive growing seasons, and analysed for pH, total organic C (C-org), extractable C (C-ext), microbial biomass-C (C-mic), 25 specific microbial activities, mean substrate-induced respiration (mSIR) for 25 substrates, functional microbial diversity and bacterial genetic diversity. No significant effect of biochar treatment was observed on C-org, C-ext, C-mic, microbial quotient (C-mic % C-org) or genetic diversity. An increase in mSIR, some specific microbial activities and soil pH, and a significant change in functional diversity were observed 3 months after treatment. In contrast, no effect of biochar was detected 14 months after treatment for the parameters considered, except for a small but significant increase in pH. Our data suggest that biochar addition stimulated soil microbial activity without causing any apparent disturbance, but this positive effect was very short-lived. (C) 2013 Published by Elsevier Masson SAS.", "keywords": ["2. Zero hunger", "Soil management", "Wood-derived biochar", "Soil bacterial diversity; Soil management; Soil microbial activity; Soil microbial functional diversity; Wheat crop; Wood-derived biochar;", "Soil microbial functional diversity", "Wheat crop", "Soil microbial activity", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Soil bacterial diversity", "15. Life on land", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1016/j.ejsobi.2013.10.007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ejsobi.2013.10.007", "name": "item", "description": "10.1016/j.ejsobi.2013.10.007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ejsobi.2013.10.007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-01-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2015.11.018", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:18:31Z", "type": "Journal Article", "created": "2016-01-08", "title": "Peat Origin And Land Use Effects On Microbial Activity, Respiration Dynamics And Exo-Enzyme Activities In Drained Peat Soils In The Netherlands", "description": "This study assessed the risk of decomposition-driven soil subsidence in drained peat soils in the Netherlands, contrasting in peat origin and current land use. In a full factorial design, fen peat and bog peat were sampled from sites in use for nature conservation and for dairy farming, which contrast in history of drainage and fertilisation. In these four peat types, which frequently occur in the Netherlands, the microbial activity and respiration dynamics were studied in samples from superficial oxic peat layers by measuring Substrate Induced Respiration (SIR) and Substrate Induced Growth Response (SIGR). Total and active microbial biomass, microbial growth potential and potential exo-enzyme activities were determined in unamended samples and after nitrogen and/or glucose amendments.<br/><br/>Remarkably, peat origin and land use did not affect basal respiration rates. In contrast, land use affected microbial biomass and potential growth rates as they were quadrupled in dairy meadows compared to nature reserves. This may be attributable to the pulses of organic and inorganic fertiliser that are being supplied in agricultural peatlands. Potential activities of oxidative exo-enzymes (phenol oxidase, POX, and phenol peroxidase, POD), in contrast, depended more on peat type, indicating a difference in peat substrate quality. Basal respiration rates and enzyme activities were not related. Phosphorus enrichment was identified as a potential driver of increased peat decomposition. The activity of the oxidative enzyme phenol oxidase and the concentration of phenolic compounds, which are considered to be the main regulators of peat decomposition according to the enzymic latch theory, were not related to respiration rates. It was concluded that decomposition theories like the enzymic latch theory (attributing a main role in the regulation of decomposition to phenolic compounds and phenol oxidase) were not supported by our research in the drained peat soils in the Netherlands.", "keywords": ["Decomposition", "Peat", "national", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Microbial activity", "Energy limitation", "13. Climate action", "Nutrient limitation", "SIR", "0401 agriculture", " forestry", " and fisheries", "SDG 2 - Zero Hunger", "SDG 15 - Life on Land", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2015.11.018"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2015.11.018", "name": "item", "description": "10.1016/j.soilbio.2015.11.018", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2015.11.018"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-04-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2016.05.004", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-06-27T16:18:31Z", "type": "Journal Article", "created": "2016-05-17", "title": "Gasification Biochar Has Limited Effects On Functional And Structural Diversity Of Soil Microbial Communities In A Temperate Agroecosystem", "description": "Abstract   Biochar may enhance soil fertility and carbon (C) sequestration but there is still a lack of comprehensive understanding of its effects on soil microbial communities and functioning. This study tested the differential effects of two doses (6\u20138 and 0.8\u20131.4\u00a0t\u00a0ha \u22121  for High and Low doses, respectively) of wheat straw gasification biochar (GBC) and fresh straw incorporated as soil amendments into an agricultural field in Denmark. Soils were analysed three months after the amendments for pH, total organic matter, microbial biomass (ATP), ten enzymatic activities, catabolic potential by substrate-induced respiration (MicroResp\u2122), soil toxicity test (BioTox\u2122) and bacterial community structure (Illumina 16S rRNA gene sequencing). No significant effect of biochar treatment was observed regarding ATP content, catabolic community profiles and soil toxicity. The higher dose of GBC increased phenol oxidase activity and soil pH, and decreased the cellulase activity. No major effect of high dose GBC was observed on the soil community diversity, and only minor effect on the community composition, with an increase in the relative abundance of a single OTU associated with  Acidobacteria_Gp16 . Addition of low dose of GBC caused an increase in the relative abundance of the rare members in the microbial communities thus increasing the diversity of soil microorganisms. A comparable effect was observed with the addition of fresh straw. Overall, our results indicated that GBC as soil amendment had a limited effect on the functional and structural diversity of soil microbial communities in a Danish temperate agroecosystem.", "keywords": ["Carbon sequestration", "2. Zero hunger", "16S Illumina sequencing", "Microbial activity", "MicroResp", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "Toxicity test", "16S Illumina sequencing; Acidobacteria; Carbon sequestration; Microbial activity; MicroResp; Toxicity test; Soil Science; Microbiology", "Acidobacteria"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2016.05.004"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2016.05.004", "name": "item", "description": "10.1016/j.soilbio.2016.05.004", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2016.05.004"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-08-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2006.05.017", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:18:21Z", "type": "Journal Article", "created": "2006-07-11", "title": "Hydrolase Activities, Microbial Biomass And Bacterial Community In A Soil After Long-Term Amendment With Different Composts", "description": "Open AccessThe use of composts in agricultural soils is a widespread practice and the positive effects on soil and plants are known from numerous studies. However, there have been few attempts to compare the effects of different kinds of composts in one single study. The aim of this paper is to investigate to what extent and to which soil depth four major types of composts would affect the soil and its microbiota. In a crop-rotation field experiment, composts produced from (i) urban organic wastes, (ii) green wastes, (iii) manure and (iv) sewage sludge were applied at a rate equivalent to 175 kg N ha\u22121 yr\u22121 for 12 years. General (total organic C (Corg), total N (Nt), microbial biomass C (Cmic), and basal respiration), specific (enzyme activities related to C, N and P cycles), biochemical properties and bacterial genetic diversity (based on DGGE analysis of 16S rDNA) were analyzed at different depths (0\u201310, 10\u201320 and 20\u201330 cm). Compost treatment increased Corg at all depths from 11 g kg\u22121 for control soil to 16.7 g kg\u22121 for the case of sewage sludge compost. Total N increased with compost treatment at 0\u201310 cm and 10\u201320 cm depths, but not at 20\u201330 cm. Basal respiration and Cmic declined with depth, and the composts resulted in an increase of Cmic and basal respiration. Enzyme activities were different depend on the enzyme and among compost treatments, but in general, the enzyme activities were higher in the upper layers (0\u201310 and 10\u201320 cm) than in the 20\u201330 cm layer. Diversity of ammonia oxidizers and bacteria was lower in the control than in the compost soils. The type of compost had less influence on the composition of the microbial communities than did soil depth.", "keywords": ["PCR-DGGE", "2. Zero hunger", "Microbial activity", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Composts", "Hydrolase activities", "04 agricultural and veterinary sciences", "15. Life on land", "Bacterial communities", "6. Clean water", "Ammonia oxidizers", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2006.05.017"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2006.05.017", "name": "item", "description": "10.1016/j.soilbio.2006.05.017", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2006.05.017"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-12-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2014.11.003", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:18:29Z", "type": "Journal Article", "created": "2014-11-17", "title": "Short- And Long-Term Effects Of Nutrient Enrichment On Microbial Exoenzyme Activity In Mangrove Peat", "description": "Abstract Mangroves receive increasing quantities of nutrients as a result of coastal development, which could lead to significant changes in carbon sequestration and soil subsidence. We hypothesised that mangrove-produced tannins induce a nitrogen (N) limitation on microbial decomposition even when plant growth is limited by phosphorus (P). As a result, increased N influx would lead to a net loss of sequestered carbon negating the ability to compensate for sea level rise in P-limited mangroves. To examine this, we quantified the short- and long-term effects of N and P enrichment on microbial biomass and decomposition-related enzyme activities in a Rhizophora mangle-dominated mangrove, which had been subjected to fertilisation treatments for a period of fifteen years. We compared microbial biomass, elemental stoichiometry and potential enzyme activity in dwarf and fringe-type R. mangle-dominated sites, where primary production is limited by P or N depending on the proximity to open water. Even in P-limited mangroves, microbial activity was N-limited as indicated by stoichiometry and an increase in enzymic activity upon N amendment. Nevertheless, microbial biomass increased upon field additions of P, indicating that the carbon supply played even a larger role. Furthermore, we found that P amendment suppressed phenol oxidase activity, while N amendment did not. The possible differential nutrient limitations of microbial decomposers versus primary producers implies that the direction of the effect of eutrophication on carbon sequestration is nutrient-specific. In addition, this study shows that phenol oxidase activities in this system decrease through P, possibly strengthening the enzymic latch effect of mangrove tannins. Furthermore, it is argued that the often used division between N-harvesting, P-harvesting, and carbon-harvesting exoenzymes needs to be reconsidered.", "keywords": ["Rhizophora", "Decomposition", "Peat", "Differential nutrient limitation", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Microbial activity", "Microbial elemental stoichiometry", "13. Climate action", "international", "Taverne", "11. Sustainability", "Mangroves", "0401 agriculture", " forestry", " and fisheries", "SDG 14 - Life Below Water", "SOC", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2014.11.003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2014.11.003", "name": "item", "description": "10.1016/j.soilbio.2014.11.003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2014.11.003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-02-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2019.107701", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:18:32Z", "type": "Journal Article", "created": "2020-01-02", "title": "Do cover crops benefit soil microbiome? A meta-analysis of current research", "description": "Abstract   Cover cropping is a promising sustainable agricultural method with the potential to enhance soil health and mitigate consequences of soil degradation. Because cover cropping can form an agroecosystem distinct from that of bare fallow, the soil microbiome is hypothesized to respond to the altered environmental circumstances. Despite the growing number of primary literature sources investigating the relationship between cover cropping and the soil microbiome, there has not been a quantitative research synthesis that is sufficiently comprehensive and specific to this relationship. We conducted a meta-analysis by compiling the results of 60 relevant studies reporting cover cropping effects on soil microbial properties to estimate global effect sizes and explore the current landscape of this topic. Overall, cover cropping significantly increased parameters of soil microbial abundance, activity, and diversity by 27%, 22%, and 2.5% respectively, compared to those of bare fallow. Moreover, cover cropping effect sizes varied by agricultural covariates like cover crop termination or tillage methods. Notably, cover cropping effects were less pronounced under conditions like continental climate, chemical cover crop termination, and conservation tillage. This meta-analysis showed that the soil microbiome can become more robust under cover cropping when properly managed with other agricultural practices. However, more primary research is still needed to control between-study heterogeneity and to more elaborately assess the relationships between cover cropping and the soil microbiome.", "keywords": ["CLIMATE", "2. Zero hunger", "MICROBIAL ACTIVITY", "13. Climate action", "SOIL ORDER", "https://purl.org/becyt/ford/4.1", "0401 agriculture", " forestry", " and fisheries", "https://purl.org/becyt/ford/4", "AGRONOMY", "MICROBIAL ABUNDANCE", "04 agricultural and veterinary sciences", "15. Life on land", "MICROBIAL DIVERSITY"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2019.107701"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2019.107701", "name": "item", "description": "10.1016/j.soilbio.2019.107701", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2019.107701"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-01T00:00:00Z"}}, {"id": "10.1016/j.still.2013.01.005", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:18:45Z", "type": "Journal Article", "created": "2013-02-27", "title": "Soil Biochemical Properties In A Semiarid Mediterranean Agroecosystem As Affected By Long-Term Tillage And N Fertilization", "description": "Tillage and N fertilization practices contribute to the balance between soil C inputs and outputs. Thus, the impacts of both practices and their interactions on soil organic C (SOC) dynamics must be studied. The main objective of this study was to determine long-term effects of tillage and N fertilization on soil biochemical properties in a long-term experiment established in 1996 on a dryland Typic Xerofluvent soil cropped with barley (Hordeum vulgare L.) in NE Spain. The response of SOC concentration, soil microbial biomass carbon (SMBC) and soil enzyme activities (DHA, dehydrogenase, and PRA, protease) to different tillage (no-tillage, NT; reduced tillage, RT; and conventional tillage, CT) and N treatments (zero, 0 kg N ha\u22121; medium, 60 kg N ha\u22121; and high, 120 kg N ha\u22121) were measured in 2008 at four soil depths (i.e., 0\u20135, 5\u201310, 10\u201325 and 25\u201350 cm). All the soil biochemical properties studied showed significant differences for tillage, depth and the interaction between tillage and soil depth. However, N fertilization rates only affected the SMBC content, which was greater under 120 kg N ha\u22121 than under 0 kg N ha\u22121 in the 10\u201325 cm soil layer. In the soil surface layer (0\u20135 cm), SOC, SMBC and DHA levels in CT were about 50% of the levels in the NT plots. However, in the 10\u201325 cm soil layer, a greater SOC concentration in CT compared with NT and RT was also accompanied by SMBC and DHA values 30% higher in CT. Below 25 cm soil depth, similar values of soil biochemical properties were found among tillage systems. There was a significant correlation among almost all the parameters studied, with the greatest correlations between SOC and SMBC and between SOC and DHA. In semiarid Mediterranean conditions, after 12 years of experiment, tillage impacted soil biochemical properties in a greater extent compared with N fertilization even though this effect was only limited to the upper soil layers. This work was supported by the Comisi\u00f3n Interministerial de Ciencia y Tecnolog\u00eda of Spain (grants AGL2007-66320-CO2-01 and AGL2010-22050-CO3-01/02) and the European Union (FEDER funds).", "keywords": ["2. Zero hunger", "Traditional tillage", "Dryland farming", "Soil microbial activity", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "Conservation tillage", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1016/j.still.2013.01.005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20and%20Tillage%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.still.2013.01.005", "name": "item", "description": "10.1016/j.still.2013.01.005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.still.2013.01.005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-05-01T00:00:00Z"}}, {"id": "10.1029/2021jg006688", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:19:16Z", "type": "Journal Article", "created": "2022-06-11", "title": "Bidirectional Exchange of Biogenic Volatile Organic Compounds in Subarctic Heath Mesocosms During Autumn Climate Scenarios", "description": "Abstract<p>Biogenic volatile organic compound (BVOC) flux dynamics during the subarctic autumn are largely unexplored and have been considered insignificant due to the relatively low biological activity expected during autumn. Here, we exposed subarctic heath ecosystems to predicted future autumn climate scenarios (ambient, warming, and colder, dark conditions), changes in light availability, and flooding, to mimic the more extreme rainfall or snowmelt events expected in the future. We used climate chambers to measure the net ecosystem fluxes and bidirectional exchange of BVOCs from intact heath mesocosms using a dynamic enclosure technique coupled to a proton\uffe2\uff80\uff90transfer\uffe2\uff80\uff90reaction time\uffe2\uff80\uff90of\uffe2\uff80\uff90flight mass spectrometer (PTR\uffe2\uff80\uff93ToF\uffe2\uff80\uff93MS). We focused on six BVOCs (methanol, acetic acid, acetaldehyde, acetone, isoprene, and monoterpenes) that were among the most dominant and that were previously identified in arctic tundra ecosystems. Warming increased ecosystem respiration and resulted in either net BVOC release or increased uptake compared to the ambient scenario. None of the targeted BVOCs showed net release in the cold and dark scenario. Acetic acid exhibited significantly lower net uptake in the cold and dark scenario than in the ambient scenario, which suggests reduced microbial activity. Flooding was characterized by net uptake of the targeted BVOCs and overruled any temperature effects conferred by the climate scenarios. Monoterpenes were mainly taken up by the mesocosms and their fluxes were not affected by the climate scenarios or flooding. This study shows that although autumn BVOC fluxes on a subarctic heath are generally low, changes in future climate may strongly modify them.</p>", "keywords": ["LITTER", "volatile organic compound", "FLUXES", "flooding", "Arctic", "Flooding", "Autumn", "11. Sustainability", "arctic", "autumn", "Ecosystem-atmosphere interactions", "Global change", "global change", "EMISSIONS", "MICROBIAL ACTIVITY", "ecosystem-atmosphere interactions", "Volatile organic compound", "15. Life on land", "FOREST", "TUNDRA", "SOIL", "NITROGEN", "SUMMER", "13. Climate action", "WINTER", "Research Article"]}, "links": [{"href": "https://agupubs.onlinelibrary.wiley.com/doi/pdf/10.1029/2021JG006688"}, {"href": "https://doi.org/10.1029/2021jg006688"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Geophysical%20Research%3A%20Biogeosciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1029/2021jg006688", "name": "item", "description": "10.1029/2021jg006688", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1029/2021jg006688"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-01T00:00:00Z"}}, {"id": "10.1038/nclimate1692", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:19:20Z", "type": "Journal Article", "created": "2013-01-31", "title": "Greenhouse-Gas Emissions From Soils Increased By Earthworms", "description": "Earthworms play an essential part in determining the greenhouse-gas balance of soils worldwide but whether their activity moves soils towards being a net source or sink remains controversial. This Review of the overall effect of earthworms on the greenhouse-gas balance of soils suggests that although beneficial to fertility, earthworms tend to increase the net soil emissions of such gases.", "keywords": ["organic-matter dynamics", "2. Zero hunger", "ecosystem engineers", "suelo", "soil fertility", "n2o emission", "earthworms", "04 agricultural and veterinary sciences", "fertilidad del suelo", "endogeic earthworms", "15. Life on land", "carbon-dioxide", "microbial activity", "soil", "12. Responsible consumption", "crop residue", "13. Climate action", "greenhouse gases", "11. Sustainability", "gases de efecto invernadero", "0401 agriculture", " forestry", " and fisheries", "nitrous-oxide fluxes", "agricultural intensification", "nitrifier denitrification", "lombriz de tierra"]}, "links": [{"href": "https://doi.org/10.1038/nclimate1692"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Climate%20Change", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/nclimate1692", "name": "item", "description": "10.1038/nclimate1692", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/nclimate1692"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-02-03T00:00:00Z"}}, {"id": "10.1046/j.1365-2486.2003.00656.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:19:37Z", "type": "Journal Article", "created": "2003-07-30", "title": "Substantial Labile Carbon Stocks And Microbial Activity In Deeply Weathered Soils Below A Tropical Wet Forest", "description": "Abstract<p>Contrary to large areas in Amazonia of tropical moist forests with a pronounced dry season, tropical wet forests in Costa Rica do not depend on deep roots to maintain an evergreen forest canopy through the year. At our Costa Rican tropical wet forest sites, we found a large carbon stock in the subsoil of deeply weathered Oxisols, even though only 0.04\uffe2\uff80\uff930.2% of the measured root biomass (&gt;2\uffe2\uff80\uff83mm diameter) to 3\uffe2\uff80\uff83m depth was below 2\uffe2\uff80\uff83m. In addition, we demonstrate that 20% or more of this deep soil carbon (depending on soil type) can be mobilized after forest clearing for pasture establishment. Microbial activity between 0.3 and 3\uffe2\uff80\uff83m depth contributed about 50% to the microbial activity in these soils, confirming the importance of the subsoil in C cycling. Depending on soil type, forest clearing for pasture establishment led from no change to a slight addition of carbon in the topsoil (0\uffe2\uff80\uff930.3\uffe2\uff80\uff83m depth). However, this effect was countered by a substantial loss of C stocks in the subsoil (1\uffe2\uff80\uff933\uffe2\uff80\uff83m depth). Our results show that large stocks of relatively labile carbon are not limited to areas with a prolonged dry season, but can also be found in deeply weathered soils below tropical wet forests. Forest clearing in such areas may produce unexpectedly high C losses from the subsoil.</p>", "keywords": ["0401 agriculture", " forestry", " and fisheries", "Costa Rica; deforestation; land-use change; microbial activity; pasture; soil organic carbon; tropical rain forest", "04 agricultural and veterinary sciences", "15. Life on land"]}, "links": [{"href": "https://doi.org/10.1046/j.1365-2486.2003.00656.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1046/j.1365-2486.2003.00656.x", "name": "item", "description": "10.1046/j.1365-2486.2003.00656.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1046/j.1365-2486.2003.00656.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-07-30T00:00:00Z"}}, {"id": "10.1111/1365-2745.12593", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:20:28Z", "type": "Journal Article", "created": "2016-04-22", "title": "Drought History Affects Grassland Plant And Microbial Carbon Turnover During And After A Subsequent Drought Event", "description": "Summary<p>   <p>Drought periods are projected to become more severe and more frequent in many European regions. While effects of single strong droughts on plant and microbial carbon (C) dynamics have been studied in some detail, impacts of recurrent drought events are still little understood.</p>  <p>We tested whether the legacy of extreme experimental drought affects responses of plant and microbial C and nitrogen (N) turnover to further drought and rewetting. In a mountain grassland, we conducted a 13C pulse\uffe2\uff80\uff90chase experiment during a naturally occurring drought and rewetting event in plots previously exposed to experimental droughts and in ambient controls (AC). After labelling, we traced 13C below\uffe2\uff80\uff90ground allocation and incorporation into soil microbes using phospholipid fatty acid biomarkers.</p>  <p>Drought history (DH) had no effects on the standing shoot and fine root plant biomass. However, plants with experimental DH displayed decreased shoot N concentrations and increased fine root N concentrations relative to those in AC. During the natural drought, plants with DH assimilated and allocated less 13C below\uffe2\uff80\uff90ground; moreover, fine root respiration was reduced and not fuelled by fresh C compared to plants in AC.</p>  <p>Regardless of DH, microbial biomass remained stable during natural drought and rewetting. Although microbial communities initially differed in their composition between soils with and without DH, they responded to the natural drought and rewetting in a similar way: gram\uffe2\uff80\uff90positive bacteria increased, while fungal and gram\uffe2\uff80\uff90negative bacteria remained stable. In soils with DH, a strongly reduced uptake of recent plant\uffe2\uff80\uff90derived 13C in microbial biomarkers was observed during the natural drought, pointing to a smaller fraction of active microbes or to a microbial community that is less dependent on plant C.</p>  <p>Synthesis. Drought history can induce changes in above\uffe2\uff80\uff90 vs. below\uffe2\uff80\uff90ground plant N concentrations and affect the response of plant C turnover to further droughts and rewetting by decreasing plant C uptake and below\uffe2\uff80\uff90ground allocation. DH does not affect the responses of the microbial community to further droughts and rewetting, but alters microbial functioning, particularly the turnover of recent plant\uffe2\uff80\uff90derived carbon, during and after further drought periods.</p>  </p>", "keywords": ["0301 basic medicine", "plant-soil (below-ground) interactions", "NITROGEN TURNOVER", "Biomass Allocation", "microbial community composition", "Negibacteria", "drought", "phospholipid fatty acid", "nitrogen", "Microbial community composition", "Plant\u2013Soil (Below\u2010ground) Interactions", "Recovery", "ROOT RESPIRATION", "Plant-soil (below-ground) interactions", "CLIMATE EXTREMES", "C pulse labelling", "Below-ground carbon allocation", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "SOIL INTERACTIONS", "below-ground carbon allocation", "C-13 pulse labelling", "Grassland", "6. Clean water", "Europe", "Phospholipid", "ORGANIC-MATTER", "Mountain Region", "Posibacteria", "DIOXIDE PULSES", "Phospholipid fatty acid", "106022 Microbiology", "Root/shoot Ratio", "Belowground Biomass", "Ecosystem Resilience", "Nitrogen", "Microbial Community", "Carbon Isotope", "Soil-vegetation Interaction", "recovery", "SUMMER DROUGHT", "03 medical and health sciences", "Rewetting", "Community Composition", "plant\u2013soil (below-ground) interactions", "WATER-STRESS", "resilience", "Drought", "Resilience", "RESILIENCE", "15. Life on land", "Turnover", "Microbial Activity", "13. Climate action", "Fatty Acid", "RESPONSES"]}, "links": [{"href": "https://doi.org/10.1111/1365-2745.12593"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1365-2745.12593", "name": "item", "description": "10.1111/1365-2745.12593", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1365-2745.12593"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-05-24T00:00:00Z"}}, {"id": "10.1111/1462-2920.15132", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:20:29Z", "type": "Journal Article", "created": "2020-06-18", "title": "Trait\u2010based approaches reveal fungal adaptations to nutrient\u2010limiting conditions", "description": "Summary<p>The dependency of microbial activity on nutrient availability in soil is only partly understood, but highly relevant for nutrient cycling dynamics. In order to achieve more insight on microbial adaptations to nutrient limiting conditions, precise physiological knowledge is needed. Therefore, we developed an experimental system assessing traits of 16 saprobic fungal isolates in nitrogen (N) limited conditions. We tested the hypotheses that (1) fungal traits are negatively affected by N deficiency to a similar extent and (2) fungal isolates respond in a phylogenetically conserved fashion. Indeed, mycelial density, spore production and fungal activity (respiration and enzymatic activity) responded similarly to limiting conditions by an overall linear decrease. By contrast, mycelial extension and hyphal elongation peaked at lowest N supply (C:N 200), causing maximal biomass production at intermediate N contents. Optimal N supply rates differed among isolates, but only the extent of growth reduction was phylogenetically conserved. In conclusion, growth responses appeared as a switch from explorative growth in low nutrient conditions to exploitative growth in nutrient\uffe2\uff80\uff90rich patches, as also supported by responses to phosphorus and carbon limitations. This detailed trait\uffe2\uff80\uff90based pattern will not only improve fungal growth models, but also may facilitate interpretations of microbial responses observed in field studies.</p>", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "Nitrogen", "Fungi", "577", "Phosphorus", "Nutrients", "Spores", " Fungal", "15. Life on land", "microbial activity", "01 natural sciences", "Carbon", "Soil", "fungal adaptations", "03 medical and health sciences", "Biomass", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::577 \u00d6kologie", "Soil Microbiology", "nutrient\u2010limiting conditions"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15132"}, {"href": "https://doi.org/10.1111/1462-2920.15132"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.15132", "name": "item", "description": "10.1111/1462-2920.15132", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.15132"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-08T00:00:00Z"}}, {"id": "10.1111/ejss.70078", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:20:35Z", "type": "Journal Article", "created": "2025-03-11", "title": "The Effect of Crop Diversification and Season on Microbial Carbon Use Efficiency Across a European Pedoclimatic Gradient", "description": "ABSTRACT<p>Microbial transformation of soil organic matter plays a critical role in carbon (C) cycling making it essential to understand how land use and management practices influence microbial physiology and its connection to C dynamics. One factor that is likely to impact soil microbial physiology is crop diversification via its influence on belowground diversity (e.g., chemical heterogeneity of C inputs, microbial community composition). However, the effect of crop diversification measures on microbial physiology and potential effects on C cycling in agricultural soils is still unclear. To address this knowledge gap, we sampled topsoil from eight experimental sites covering different crop diversification measures across Europe (i.e., cover crops, ley farming, vegetation stripes). We used the 18O\uffe2\uff80\uff90labelling method to analyse microbial C use efficiency (CUE), growth, respiration and biomass C. Additionally, a second sampling at five selected sites examined whether the growing season influenced the impact of crop diversification. Meta\uffe2\uff80\uff90analysis revealed no overall effect of crop diversification on CUE, microbial activity, biomass or soil organic C (SOC). However, the effects varied with the type of diversification measure: cover crops did not affect carbon processing, vegetation stripes increased microbial activity, and ley farming enhanced CUE. The largest variation in CUE was observed between samplings at the same sites, indicating seasonal dynamics. Temperature, precipitation and photosynthetically active radiation predicted seasonal variation in CUE (R2\uffe2\uff80\uff89=\uffe2\uff80\uff890.36). While cover crops did not significantly impact C storage in our study, both ley farming and vegetation stripes increased SOC. The overall effect of crop diversification on SOC seems to be decoupled from highly temporally variable CUE in the bulk soil and rather relate to C\uffe2\uff80\uff90inputs.</p", "keywords": ["[SDE] Environmental Sciences", "570", "ley farming", "microbial activity", "pedoclimatic gradient", "630", "[SDV] Life Sciences [q-bio]", "O-CUE", "soil organic carbon", "18 O-CUE", "[SDE]Environmental Sciences", "vegetation stripes", "cover crops", "[SDV.SA.SDS] Life Sciences [q-bio]/Agricultural sciences/Soil study", "EJP-SOIL", "microbial physiology"]}, "links": [{"href": "https://doi.org/10.1111/ejss.70078"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/ejss.70078", "name": "item", "description": "10.1111/ejss.70078", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/ejss.70078"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-24T00:00:00Z"}}, {"id": "10.1111/nph.19112", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:21:08Z", "type": "Journal Article", "created": "2023-06-29", "title": "Gold\u2010FISH enables targeted NanoSIMS analysis of plant\u2010associated bacteria", "description": "Summary<p> <p>Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of in\uffc2\uffa0situ evidence of bacterial activity.</p> <p>To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold\uffe2\uff80\uff90based in\uffc2\uffa0situ hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity.</p> <p>We incubated Kosakonia strain DS\uffe2\uff80\uff901\uffe2\uff80\uff90associated, gnotobiotically grown rice plants with 15N\uffe2\uff80\uff93N2 gas to detect in\uffc2\uffa0situ N2 fixation activity. Bacterial cells along the rhizoplane showed\uffc2\uffa0heterogeneous patterns of 15N enrichment, ranging from the natural isotope abundance levels up to 12.07 at% 15N (average and median of 3.36 and 2.85 at% 15N, respectively, n\uffe2\uff80\uff89=\uffe2\uff80\uff89697 cells).</p> <p>The presented correlative optical and chemical imaging analysis is applicable to a broad range of studies investigating plant\uffe2\uff80\uff93microbe interactions. For example, it enables verification of the in\uffc2\uffa0situ metabolic activity of host\uffe2\uff80\uff90associated commercialized strains or plant growth\uffe2\uff80\uff90promoting bacteria, thereby disentangling their role in plant nutrition. Such data facilitate the design of plant\uffe2\uff80\uff93microbe combinations for improvement of crop management.</p> </p", "keywords": ["2. Zero hunger", "106022 Mikrobiologie", "rhizosphere bacteria", "Bacteria", "plant growth-promoting bacteria", "plant\u2013microbe interaction", "Research", "Oryza", "biological nitrogen fixation", "Plants", "microbial activity", "in\u00a0situ hybridization", "Plant Roots", "106026 \u00d6kosystemforschung", "Rhizosphere", "106022 Microbiology", "NanoSIMS", "in situ hybridization", "106026 Ecosystem research", "In situ hybridization", "In Situ Hybridization", "Soil Microbiology", "plant-microbe interaction"]}, "links": [{"href": "https://doi.org/10.1111/nph.19112"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.19112", "name": "item", "description": "10.1111/nph.19112", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.19112"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-06-28T00:00:00Z"}}, {"id": "10.1111/sum.13150", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:21:10Z", "type": "Journal Article", "created": "2024-12-09", "title": "Long\u2010term conservation tillage in organic farming maintains sandy loam soil functioning despite increased penetration resistance", "description": "Abstract<p>Conservation tillage is an attractive practice in organic farming; however, it requires more trafficking for weed management than conventional tillage. This scenario can lead to soil compaction below the tillage working depth. However, it is not clear whether long\uffe2\uff80\uff90term conservation tillage practices impair soil functions. Therefore, this study investigated whether long\uffe2\uff80\uff90term conservation tillage causes soil compaction and impairs water retention, gaseous exchange capability and microbial activity of the soil below the tillage working depth. A long\uffe2\uff80\uff90term (16\uffe2\uff80\uff89years) organic farming experiment consisting of conservation tillage (VST, shallow tillage to 7\uffe2\uff80\uff89cm depth) and conventional tillage (MP, mouldboard ploughing to 30\uffe2\uff80\uff89cm depth) was conducted on a sandy loam soil. Soil penetration resistance (PR), saturated hydraulic conductivity (SHC), water retention, gaseous exchange properties and microbial activity were determined in the 20\uffe2\uff80\uff9330\uffe2\uff80\uff89cm soil layer. The PR did not significantly differ between the tillage treatments at any depth; however, VST recorded 30%\uffe2\uff80\uff9363% higher PR values in the 10\uffe2\uff80\uff9330\uffe2\uff80\uff89cm soil layer. Among the measured soil properties, only microbial activity could yield a statistical difference between the two tillage practices. The water retention behaviour, plant available water capacity, SHC and relative gas diffusivity were very similar under both tillage treatments. The mean effective air\uffe2\uff80\uff90filled porosity (E\uffe2\uff80\uff90AFP) at pF 1.5 was 21% lower under VST than that under MP; however, the difference was reduced to 3% at pF 2. At field capacity, the E\uffe2\uff80\uff90AFP was approximately 14%\uffe2\uff80\uff94above the critical limit (10%) for plant growth\uffe2\uff80\uff94under both tillage practices. Air permeability was 76% and 57% higher under VST at pF 1.5 and 1.7, respectively, compared with MP. The microbial activity was 56% lower (p\uffe2\uff80\uff89=\uffe2\uff80\uff89.04) under VST than that under MP. Likewise, 31%, 65% and 34% higher microbial biomass carbon, microbial biomass nitrogen and dissolved organic carbon, respectively, were observed under MP compared with VST. These results indicated that despite higher penetration resistance, long\uffe2\uff80\uff90term conservation tillage did not limit water retention and aeration capability of soil in the 20\uffe2\uff80\uff9330\uffe2\uff80\uff89cm soil layer. However, long\uffe2\uff80\uff90term conservation tillage may induce vertical stratification of organic matter and microbial activity, which have implications for crop production.</p", "keywords": ["soil compaction", "soil organic carbon", "air permeability", "relative gas diffusivity", "shallow tillage", "air-filled porosity", "microbial activity", "water retention"]}, "links": [{"href": "https://doi.org/10.1111/sum.13150"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Use%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/sum.13150", "name": "item", "description": "10.1111/sum.13150", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/sum.13150"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-01T00:00:00Z"}}, {"id": "10.1128/aem.00033-11", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:21:13Z", "type": "Journal Article", "created": "2011-04-23", "title": "Association Of Earthworm-Denitrifier Interactions With Increased Emission Of Nitrous Oxide From Soil Mesocosms Amended With Crop Residue", "description": "ABSTRACT           <p>             Earthworm activity is known to increase emissions of nitrous oxide (N             2             O) from arable soils. Earthworm gut, casts, and burrows have exhibited higher denitrification activities than the bulk soil, implicating priming of denitrifying organisms as a possible mechanism for this effect. Furthermore, the earthworm feeding strategy may drive N             2             O emissions, as it determines access to fresh organic matter for denitrification. Here, we determined whether interactions between earthworm feeding strategy and the soil denitrifier community can predict N             2             O emissions from the soil. We set up a 90-day mesocosm experiment in which             15             N-labeled maize (             Zea mays             L.) was either mixed in or applied on top of the soil in the presence or absence of the epigeic earthworm             Lumbricus rubellus             and/or the endogeic earthworm             Aporrectodea caliginosa             . We measured N             2             O fluxes and tested the bulk soil for denitrification enzyme activity and the abundance of 16S rRNA and denitrifier genes             nirS             and             nosZ             through real-time quantitative PCR. Compared to the control,             L. rubellus             increased denitrification enzyme activity and N             2             O emissions on days 21 and 90 (day 21,             P             = 0.034 and             P             = 0.002, respectively; day 90,             P             = 0.001 and             P             = 0.007, respectively), as well as cumulative N             2             O emissions (76%;             P             = 0.014).             A. caliginosa             activity led to a transient increase of N             2             O emissions on days 8 to 18 of the experiment. Abundance of             nosZ             was significantly increased (100%) on day 90 in the treatment mixture containing             L. rubellus             alone. We conclude that             L. rubellus             increased cumulative N             2             O emissions by affecting denitrifier community activity via incorporation of fresh residue into the soil and supplying a steady, labile carbon source.           </p>", "keywords": ["2. Zero hunger", "agricultural soil", "Bacteria", "nosz genes", "carbon", "Nitrous Oxide", "n2o emission", "n2o-producing microorganisms", "04 agricultural and veterinary sciences", "15. Life on land", "pcr data", "microbial activity", "Animal Feed", "Zea mays", "lumbricus-rubellus", "Soil", "Denitrification", "Animals", "0401 agriculture", " forestry", " and fisheries", "community composition", "Oligochaeta", "organic-matter"]}, "links": [{"href": "https://doi.org/10.1128/aem.00033-11"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20and%20Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/aem.00033-11", "name": "item", "description": "10.1128/aem.00033-11", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/aem.00033-11"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-06-15T00:00:00Z"}}, {"id": "10.1371/journal.pone.0070224", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:21:38Z", "type": "Journal Article", "created": "2013-07-16", "title": "Effects Of Added Organic Matter And Water On Soil Carbon Sequestration In An Arid Region", "description": "Open AccessEn general, se predice que el calentamiento global estimular\u00e1 la producci\u00f3n primaria y conducir\u00e1 a m\u00e1s aportes de carbono (C) al suelo. Sin embargo, muchos estudios han encontrado que el suelo C no necesariamente aumenta con el aumento de la entrada de basura vegetal. Las precipitaciones han aumentado en Asia central \u00e1rida y se prev\u00e9 que aumenten m\u00e1s, por lo que probamos los efectos de la adici\u00f3n de materia org\u00e1nica fresca (FOM) y agua en el secuestro de C del suelo en una regi\u00f3n \u00e1rida en el noroeste de China. Los resultados sugirieron que el FOM a\u00f1adido se descompuso r\u00e1pidamente y tuvo efectos menores en el dep\u00f3sito de carbono org\u00e1nico del suelo (SOC) a una profundidad de 30 cm. Tanto la FOM como la adici\u00f3n de agua tuvieron efectos significativos en la biomasa microbiana del suelo. La biomasa microbiana del suelo aument\u00f3 con la adici\u00f3n de FOM, alcanz\u00f3 un m\u00e1ximo y luego disminuy\u00f3 a medida que la FOM se descompon\u00eda. El FOM tuvo un efecto estimulante m\u00e1s significativo sobre la biomasa microbiana con la adici\u00f3n de agua. Bajo los rangos de humedad del suelo utilizados en este experimento (21.0% -29.7%), el aporte de FOM fue m\u00e1s importante que la adici\u00f3n de agua en el proceso de mineralizaci\u00f3n del suelo C. Concluimos que la entrada de FOM a corto plazo en el suelo subterr\u00e1neo y la adici\u00f3n de agua no afectan la piscina de SOC en los matorrales en una regi\u00f3n \u00e1rida.", "keywords": ["Carbon sequestration", "550", "Arid", "Growth", "630", "Agricultural and Biological Sciences", "Soil", "Agricultural soil science", "Tropical forest", "Soil water", "Carbon fibers", "Biomass", "Land-use", "2. Zero hunger", "Analysis of Land Cover and Ecosystems", "Ecology", "Respiration", "Q", "Temperature", "R", "Soil Chemical Properties", "Life Sciences", "Composite number", "04 agricultural and veterinary sciences", "Soil carbon", "6. Clean water", "Chemistry", "Physical Sciences", "Environmental chemistry", "Medicine", "Organic matter", "Research Article", "Composite material", "Carbon Sequestration", "China", "Desert shrubs", "Science", "Soil Science", "Ecosystems", "Environmental science", "Meta-analysis in Ecology and Agriculture Research", "Organic Matter Dynamics", "Climate-change", "Soil Carbon Sequestration", "Biology", "Ecology", " Evolution", " Behavior and Systematics", "Soil science", "Soil organic matter", "Soil Fertility", "Water", "Soil Properties", "15. Life on land", "Soil biodiversity", "Materials science", "Microbial activity", "Carbon dioxide", "13. Climate action", "FOS: Biological sciences", "Environmental Science", "Fine-root", "0401 agriculture", " forestry", " and fisheries", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "CO2 flux"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0070224"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0070224", "name": "item", "description": "10.1371/journal.pone.0070224", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0070224"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-07-16T00:00:00Z"}}, {"id": "10.3390/genes13050850", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:23:34Z", "type": "Journal Article", "created": "2022-05-11", "title": "Short-Term Responses of Soil Microbial Communities to Changes in Air Temperature, Soil Moisture and UV Radiation", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>We analyzed the effects on a soil microbial community of short-term alterations in air temperature, soil moisture and ultraviolet radiation and assessed the role of invertebrates (species Enchytraeus crypticus) in modulating the community\u2019s response to these factors. The reference soil, Lufa 2.2, was incubated for 48 h, with and without invertebrates, under the following conditions: standard (20 \u00b0C + 50% water holding capacity (WHC)); increased air temperature (15\u201325 \u00b0C or 20\u201330 \u00b0C + 50% WHC); flood (20 \u00b0C + 75% WHC); drought (20 \u00b0C + 25% WHC); and ultraviolet radiation (UV) (20 \u00b0C + 50% WHC + UV). BIOLOG EcoPlates and 16S rDNA sequencing (Illumina) were used to assess the microbial community\u2019s physiological profile and the bacterial community\u2019s structure, respectively. The bacterial abundance (estimated by 16S rDNA qPCR) did not change. Most of the conditions led to an increase in microbial activity and a decrease in diversity. The structure of the bacterial community was particularly affected by higher air temperatures (20\u201330 \u00b0C, without E. crypticus) and floods (with E. crypticus). Effects were observed at the class, genera and OTU levels. The presence of invertebrates mostly resulted in the attenuation of the observed effects, highlighting the importance of considering microbiome\u2013invertebrate interactions. Considering future climate changes, the effects described here raise concern. This study provides fundamental knowledge to develop effective strategies to mitigate these negative outcomes. However, long-term studies integrating biotic and abiotic factors are needed.</p></article>", "keywords": ["0301 basic medicine", "Soil invertebrates", "Ultraviolet Rays", "drought", "microbial activity", "DNA", " Ribosomal", "Flood", "Article", "Quantitative PCR", "Soil", "03 medical and health sciences", "soil microbiome", "2. Zero hunger", "metagenomics", "increased temperature; drought; flood; UV exposure; microbial activity; bacterial diversity; metagenomics; quantitative PCR; soil microbiome; soil invertebrates", "Soil microbiome", "0303 health sciences", "Drought", "Bacteria", "Microbiota", "bacterial diversity", "Temperature", "Water", "flood", "15. Life on land", "soil invertebrates", "6. Clean water", "UV exposure", "Microbial activity", "Bacterial diversity", "13. Climate action", "quantitative PCR", "Metagenomics", "Increased temperature", "increased temperature"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/13/5/850/pdf"}, {"href": "https://doi.org/10.3390/genes13050850"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes13050850", "name": "item", "description": "10.3390/genes13050850", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes13050850"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-05-10T00:00:00Z"}}, {"id": "10.1590/s0100-06832011000600035", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:21:57Z", "type": "Journal Article", "created": "2012-02-01", "title": "Microbial And Soil Properties In Restoration Areas In The Jequitinhonha Valley, Minas Gerais", "description": "<p>To mitigate the impacts of eucalypt monoculture, forestry companies in the Upper Jequitinhonha Valley (MG) have adopted the insertion of strips of native vegetation in-between the commercial plantations. The method used for the creation of these corridors is to allow spontaneous regrowth of native vegetation in areas previously under eucalypt. The objective of this study was to evaluate the effect of cover crops on microbial and soil properties for a detailed description of the restoration process of native vegetation in forest soils of the Jequitinhonha Valley. The treatments were represented by an initial restoration stage (&lt; 4 years) with or without remaining eucalypt and the advanced restoration stage (&gt; 4 years) with or without remaining eucalypt, plus the three controls: commercial eucalypt plantation, Cerrado vegetation and native forest. Soil samples were collected for three consecutive years in the dry and rainy season (August and February, respectively). The microbial activity, regardless of the presence of remaining eucalypt , did not differ among the restoration areas, except for the metabolic quotient (qCO2) in the rainy season of February 2007. At this time, this microbial activity was higher in the advanced restoration stage without eucalypt than initial restoration without eucalypt and advanced restoration with eucalypt. The restoration areas, in general, did not differ from the control: eucalypt plantation and Cerrado either. Compared to the forest, the levels of organic C, microbial C, basal respiration (Rbasal) and hydrolysis of fluorescein diacetate (FDA) in the restoration areas were, in general, lower and did not differ in qCO2 and microbial quotient (qMIC). In general, the soil quality was similar in the initial and advanced restoration stages. Most of the soil and microbial properties in the three years indicated that the restoration areas were most similar to the Cerrado. In the advanced restoration areas without eucalypt compared to Cerrado, the lower Rbasal in the 3rd year and the lower FDA and qMIC and higher qCO2 in the 2nd year indicated that the removal of the remaining eucalypt trees was unfavorable for restoration.</p>", "keywords": ["microbial biomass", "revegeta\u00e7\u00e3o espont\u00e2nea", "Agriculture (General)", "qualidade de solo", "\u00e1reas degradadas", "04 agricultural and veterinary sciences", "15. Life on land", "microbial activity", "degraded areas", "atividade microbiana", "S1-972", "biomassa microbiana", "spontaneous regrowth", "0401 agriculture", " forestry", " and fisheries", "soil quality"]}, "links": [{"href": "https://doi.org/10.1590/s0100-06832011000600035"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20Brasileira%20de%20Ci%C3%AAncia%20do%20Solo", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0100-06832011000600035", "name": "item", "description": "10.1590/s0100-06832011000600035", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0100-06832011000600035"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-12-01T00:00:00Z"}}, {"id": "10.1590/s0100-06832013000600006", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:21:57Z", "type": "Journal Article", "created": "2014-03-26", "title": "Chemical And Biological Properties Of Phosphorus-Fertilized Soil Under Legume And Grass Cover (Cerrado Region, Brazil)", "description": "<p>The use of cover crops has been suggested as an effective method to maintain and/or increase the organic matter content, while maintaining and/or enhancing the soil physical, chemical and biological properties. The fertility of Cerrado soils is low and, consequently, phosphorus levels as well. Phosphorus is required at every metabolic stage of the plant, as it plays a role in the processes of protein and energy synthesis and influences the photosynthetic process. This study evaluated the influence of cover crops and phosphorus rates on soil chemical and biological properties after two consecutive years of common bean. The study analyzed an Oxisol in Selv\uffc3\uffadria (Mato Grosso do Sul, Brazil), in a randomized block, split plot design, in a total of 24 treatments with three replications. The plot treatments consisted of cover crops (millet, pigeon pea, crotalaria, velvet bean, millet + pigeon pea, millet + crotalaria, and millet + velvet bean) and one plot was left fallow. The subplots were represented by phosphorus rates applied as monoammonium phosphate (0, 60 and 90 kg ha-1 P2O5). In August 2011, the soil chemical properties were evaluated (pH, organic matter, phosphorus, potential acidity, cation exchange capacity, and base saturation) as well as biological variables (carbon of released CO2, microbial carbon, metabolic quotient and microbial quotient). After two years of cover crops in rotation with common bean, the cover crop biomass had not altered the soil chemical properties and barely influenced the microbial activity. The biomass production of millet and crotalaria (monoculture or intercropped) was highest. The biological variables were sensitive and responded to increasing phosphorus rates with increases in microbial carbon and reduction of the metabolic quotient.</p>", "keywords": ["2. Zero hunger", "carbono microbiano", "Agriculture (General)", "Cerrado", "04 agricultural and veterinary sciences", "15. Life on land", "aduba\u00e7\u00e3o verde", "microbial activity", "cover plants", "6. Clean water", "atividade microbiana", "mat\u00e9ria org\u00e2nica", "S1-972", "cerrado", "microbial biomass carbon", "0401 agriculture", " forestry", " and fisheries", "organic matter"]}, "links": [{"href": "https://doi.org/10.1590/s0100-06832013000600006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20Brasileira%20de%20Ci%C3%AAncia%20do%20Solo", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0100-06832013000600006", "name": "item", "description": "10.1590/s0100-06832013000600006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0100-06832013000600006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-12-01T00:00:00Z"}}, {"id": "10.1590/s0100-204x2009000600012", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:21:58Z", "type": "Journal Article", "created": "2009-08-13", "title": "Biological Indicator Attributes Of Soil Quality Under Cultivated And Natural Pasture In The Pantanal Wetlands", "description": "<p>O objetivo deste trabalho foi avaliar as altera\uffc3\uffa7\uffc3\uffb5es nos atributos biol\uffc3\uffb3gicos do solo, em raz\uffc3\uffa3o da convers\uffc3\uffa3o floresta nativa pastagem cultivada e submiss\uffc3\uffa3o de pastagens nativas a sistema pastejo cont\uffc3\uffadnuo, um Neossolo Quartzar\uffc3\uffaanico, no Pantanal sul-mato-grossense. O trabalho consistiu avalia\uffc3\uffa7\uffc3\uffa3o tr\uffc3\uffaas florestas nativas; Brachiaria decumbens com diferentes idades de forma\uffc3\uffa7\uffc3\uffa3o, implantadas em substitui\uffc3\uffa7\uffc3\uffa3o \uffc3\uffa0s florestas nativas; e tr\uffc3\uffaas pastagens nativas (uma sob sistema de pastejo cont\uffc3\uffadnuo e duas sem pastejo por 3 e 19 anos). Foram coletadas amostras de solo nas profundidades 0-10 e 10-20 cm, em tr\uffc3\uffaas transectos de 100 m estabelecidos em cada ambiente de estudo, cada um dos quais constituiu uma repeti\uffc3\uffa7\uffc3\uffa3o. Os atributos avaliados foram: carbono org\uffc3\uffa2nico total (COT), carbono microbiano (Cmic), quociente microbiano (qMIC), respira\uffc3\uffa7\uffc3\uffa3o basal (RB) e quociente metab\uffc3\uffb3lico (qCO2). A substitui\uffc3\uffa7\uffc3\uffa3o da floresta nativa por pastagem cultivada promoveu redu\uffc3\uffa7\uffc3\uffa3o nos teores de COT, Cmic e qMIC e eleva\uffc3\uffa7\uffc3\uffa3o da RB; o sistema de pastejo cont\uffc3\uffadnuo em pastagem nativa promoveu redu\uffc3\uffa7\uffc3\uffa3o nos teores de COT e no Cmic. O Cmic \uffc3\uffa9 o atributo mais sens\uffc3\uffadvel \uffc3\uffa0s altera\uffc3\uffa7\uffc3\uffb5es impostas ao solo, pelos sistemas de pastagens cultivada e nativa, pois foi marcado por redu\uffc3\uffa7\uffc3\uffb5es mais expressivas.</p>", "keywords": ["microbial quotient", "carbono microbiano", "04 agricultural and veterinary sciences", "quociente metab\u00f3lico", "microbial activity", "01 natural sciences", "atividade microbiana", "microbial biomass carbon", "basal respiration", "respira\u00e7\u00e3o basal", "0401 agriculture", " forestry", " and fisheries", "metabolic quotient", "quociente microbiano", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Cardoso, Evaldo Luis, Silva, Marx Leandro Naves, Moreira, F\u00e1tima Maria de Souza, Curi, Nilton,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1590/s0100-204x2009000600012"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Pesquisa%20Agropecu%C3%A1ria%20Brasileira", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0100-204x2009000600012", "name": "item", "description": "10.1590/s0100-204x2009000600012", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0100-204x2009000600012"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-06-01T00:00:00Z"}}, {"id": "10.1590/s0103-90162011000200013", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:21:59Z", "type": "Journal Article", "created": "2011-04-07", "title": "Soil Biochemistry And Microbial Activity In Vineyards Under Conventional And Organic Management At Northeast Brazil", "description": "<p>The S\uffc3\uffa3o Francisco Submedium Valley is located at the Brazilian semiarid region and is an important center for irrigated fruit growing. This region is responsible for 97% of the national exportation of table grapes, including seedless grapes. Based on the fact that organic fertilization can improve soil quality, we compared the effects of conventional and organic soil management on microbial activity and mycorrhization of seedless grape crops. We measured glomerospores number, most probable number (MPN) of propagules, richness of arbuscular mycorrhizal fungi (AMF) species, AMF root colonization, EE-BRSP production, carbon microbial biomass (C-MB), microbial respiration, fluorescein diacetate hydrolytic activity (FDA) and metabolic coefficient (qCO2). The organic management led to an increase in all variables with the exception of EE-BRSP and qCO2. Mycorrhizal colonization increased from 4.7% in conventional crops to 15.9% in organic crops. Spore number ranged from 4.1 to 12.4 per 50 g-1 soil in both management systems. The most probable number of AMF propagules increased from 79 cm-3 soil in the conventional system to 110 cm-3 soil in the organic system. Microbial carbon, CO2 emission, and FDA activity were increased by 100 to 200% in the organic crop. Thirteen species of AMF were identified, the majority in the organic cultivation system. Acaulospora excavata, Entrophospora infrequens, Glomus sp.3 and Scutellospora sp. were found only in the organically managed crop. S. gregaria was found only in the conventional crop. Organically managed vineyards increased mycorrhization and general soil microbial activity.</p>", "keywords": ["semiarid", "sustainable agriculture", "Soil", "atividade microbiana do solo", "semi-\u00e1rido", "agricultura sustent\u00e1vel", "Vitis vinifera L.", "0401 agriculture", " forestry", " and fisheries", "arbuscular mycorrhizal fungi", "04 agricultural and veterinary sciences", "soil microbial activity", "fungos micorr\u00edzicos arbusculares"]}, "links": [{"href": "https://doi.org/10.1590/s0103-90162011000200013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientia%20Agricola", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0103-90162011000200013", "name": "item", "description": "10.1590/s0103-90162011000200013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0103-90162011000200013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-04-01T00:00:00Z"}}, {"id": "10.1590/s1413-70542008000200001", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:21:59Z", "type": "Journal Article", "created": "2008-08-12", "title": "Avalia\u00e7\u00e3o De Indicadores Biol\u00f3gicos De Qualidade Do Solo Sob Sistemas De Cultivo Convencional E Org\u00e2nico De Frutas", "description": "<p>Objetivou-se com este trabalho avaliar os indicadores biol\uffc3\uffb3gicos de qualidade do solo sob sistemas de cultivo convencional e org\uffc3\uffa2nico de frutas no estado do Piau\uffc3\uffad. Amostras de solo foram coletadas na proje\uffc3\uffa7\uffc3\uffa3o da copa das plantas e nas entrelinhas nas profundidades de 0-10, 10-20 e 20-40 cm em duas \uffc3\uffa1reas: sistema org\uffc3\uffa2nico com acerola (SO), sistema convencional com goiaba (SC). Uma \uffc3\uffa1rea adjacente com vegeta\uffc3\uffa7\uffc3\uffa3o nativa (AVN) foi utilizada como referencial, sendo coletadas amostras de solo nas mesmas profundidades em toda \uffc3\uffa1rea. As vari\uffc3\uffa1veis analisadas foram a respira\uffc3\uffa7\uffc3\uffa3o basal, carbono org\uffc3\uffa2nico e microbiano e os quocientes respirat\uffc3\uffb3rio e microbiano do solo. O maior valor de respira\uffc3\uffa7\uffc3\uffa3o basal foi observado no solo do SOcopa, na profundidade de 10-20 cm. Os solos amostrados na \uffc3\uffa1rea de vegeta\uffc3\uffa7\uffc3\uffa3o nativa (AVN) e sob a copa das plantas no sistema de cultivo org\uffc3\uffa2nico (SOcopa) apresentaram teores elevados de Corg na superf\uffc3\uffadcie (0-10 cm) e em profundidade (20-40 cm). Em rela\uffc3\uffa7\uffc3\uffa3o ao solo da AVN, houve aumento no carbono da biomassa microbiana (Cmic), nas profundidades de 0-10 cm e 10-20 cm, para o SOcopa e SCcopa. A ado\uffc3\uffa7\uffc3\uffa3o do sistema org\uffc3\uffa2nico aumentou a atividade microbiana e o conte\uffc3\uffbado de carbono org\uffc3\uffa2nico do solo, mostrando benef\uffc3\uffadcios para esse sistema agr\uffc3\uffadcola.</p>", "keywords": ["Sustainability", "Sustentabilidade", "microrganismos", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "microorganisms", "microbial activity", "atividade microbiana"], "contacts": [{"organization": "Sampaio, Deusiane Batista, Ara\u00fajo, Ademir S\u00e9rgio Ferreira de, Santos, Valdinar Bezerra dos,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1590/s1413-70542008000200001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ci%C3%AAncia%20e%20Agrotecnologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s1413-70542008000200001", "name": "item", "description": "10.1590/s1413-70542008000200001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s1413-70542008000200001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-04-01T00:00:00Z"}}, {"id": "10.1590/s1413-70542009000400010", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:21:59Z", "type": "Journal Article", "created": "2010-01-08", "title": "Indicadores Biol\u00f3gicos De Qualidade Do Solo Em Diferentes Sistemas De Uso No Brejo Paraibano", "description": "<p>A avalia\uffc3\uffa7\uffc3\uffa3o da qualidade do solo \uffc3\uffa9 uma ferramenta importante para monitorar a sua degrada\uffc3\uffa7\uffc3\uffa3o, bem como planejar a implanta\uffc3\uffa7\uffc3\uffa3o de pr\uffc3\uffa1ticas sustent\uffc3\uffa1veis de manejo. Neste trabalho, objetivou-se avaliar indicadores biol\uffc3\uffb3gicos de qualidade do solo em um Latossolo Amarelo distr\uffc3\uffb3fico, submetido a diferentes sistemas de uso em Areia-PB. Foram utilizadas amostras de solo coletadas na camada ar\uffc3\uffa1vel (0-20 cm) em \uffc3\uffa1reas de mata nativa, fruticultura, cana-de-a\uffc3\uffa7\uffc3\uffbacar, sucess\uffc3\uffa3o de cultivos, pastagem e cons\uffc3\uffb3rcio de culturas. Foram avaliados o carbono org\uffc3\uffa2nico total (COT), carbono da biomassa microbiana (CBM), quociente microbiano (qMic), respira\uffc3\uffa7\uffc3\uffa3o basal (RB) e quociente metab\uffc3\uffb3lico (qCO2). Os resultados obtidos indicaram que as \uffc3\uffa1reas sob gram\uffc3\uffadneas apresentaram uma tend\uffc3\uffaancia de manuten\uffc3\uffa7\uffc3\uffa3o do COT e menores redu\uffc3\uffa7\uffc3\uffb5es no CBM, entretanto, apresentaram elevados valores de qCO2, indicando a ocorr\uffc3\uffaancia de um processo degradativo. A \uffc3\uffa1rea sob fruticultura apresentou leves redu\uffc3\uffa7\uffc3\uffb5es no COT e CBM e baixos valores de qCO2, indicando que esse sistema pode estar se ajustando a um novo estado de equil\uffc3\uffadbrio. As \uffc3\uffa1reas sob sucess\uffc3\uffa3o de cultivos e cons\uffc3\uffb3rcio de culturas apresentaram as maiores redu\uffc3\uffa7\uffc3\uffb5es no COT e CBM e elevados valores de qCO2, demonstrando um elevado est\uffc3\uffa1gio de degrada\uffc3\uffa7\uffc3\uffa3o desses sistemas. Pode-se concluir que todos os sistemas agr\uffc3\uffadcolas estudados promoveram perda de qualidade do solo, sendo esse fato mais pronunciado nas \uffc3\uffa1reas sob manejo mais intensivo. O CBM e qCO2 mostraram-se bastantes sens\uffc3\uffadveis \uffc3\uffa0s altera\uffc3\uffa7\uffc3\uffb5es decorrentes do uso agr\uffc3\uffadcola do solo, apresentando grande potencial para estudos de sua qualidade.</p>", "keywords": ["2. Zero hunger", "microbial quotient", "microbial biomass", "Agroecosystem", "04 agricultural and veterinary sciences", "15. Life on land", "quociente metab\u00f3lico", "microbial activity", "Agroecossistema", "6. Clean water", "atividade microbiana", "biomassa microbiana", "0401 agriculture", " forestry", " and fisheries", "metabolic quotient", "quociente microbiano"]}, "links": [{"href": "https://doi.org/10.1590/s1413-70542009000400010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ci%C3%AAncia%20e%20Agrotecnologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s1413-70542009000400010", "name": "item", "description": "10.1590/s1413-70542009000400010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s1413-70542009000400010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-08-01T00:00:00Z"}}, {"id": "10.1590/s1415-43662012000500004", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:22:00Z", "type": "Journal Article", "created": "2012-04-28", "title": "Soil Microbial Biomass Under Different Tillage And Levels Of Applied Pig Slurry", "description": "<p>ABSTRACT The objective of this work was to evaluate the changes in microbial biomass C, N and P due to the application of pig slurry under different soil tillage systems. The experiment was established in a clayey Oxisol, Eutrophic Red Latossol in Palotina, PR. Different quantities of pig slurry (0, 30, 60 and 120 m3 ha-1 year-1) were applied to the soil prior to the summer and winter crop season under conventional tillage (CT) and no tillage (NT), in three replicates. The area was cultivated with soybean (Glycine max L.) or maize (Zea mays L.) in the summer and wheat (Triticum sativum Lam.) or oat (Avena sativa L.) in the winter. The soil samples were collected in March and October of 1998 and 1999 at depths of 0-5, 5-10 and 10-20 cm. The soil tillage and pig slurry application influenced the microbial biomass C, N and P. The microbial biomass and the microbial activity presented high sensibility to detect changes in the soil due to tillage and the application of pig slurry. The soil microbial biomass and Cmic/Corg relation increased as the quantity of applied pig slurry increased. The metabolic quotient under CT increased with depth while under NT it decreased. The soil microbial biomass was enriched in N and P under NT and as the quantity of applied pig slurry increased.</p>", "keywords": ["2. Zero hunger", "plantio direto", "swine manure", "res\u00c3duos de su\u00c3nos", "0401 agriculture", " forestry", " and fisheries", "nutrient cycling", "ciclagem de nutrientes", "04 agricultural and veterinary sciences", "15. Life on land", "soil management", "microbial activity", "6. Clean water", "atividade microbiana"], "contacts": [{"organization": "Balota, Elcio L., Machineski, Oswaldo, Matos, Maria A.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1590/s1415-43662012000500004"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20Brasileira%20de%20Engenharia%20Agr%C3%ADcola%20e%20Ambiental", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s1415-43662012000500004", "name": "item", "description": "10.1590/s1415-43662012000500004", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s1415-43662012000500004"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-05-01T00:00:00Z"}}, {"id": "10.17221/567/2015-pse", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:22:05Z", "type": "Journal Article", "created": "2016-01-05", "title": "Soil Characteristics And Crop Yields Under Different Tillage Techniques", "description": "The field experiment with different soil tillage treatments has been carried out in Prague-Ruzyn\u011b locality (Czech Republic) since 1995. Data of two growing cycles in the years 2007-2010 and 2011-2014 were evaluated. Tillage technique was decisive for changes in soil characteristics and crop yields. Bulk density, organic carbon (Corg) and microbial biomass C (Cmic) were more equilibrated throughout all tested soil layers (0-0.1; 0.1-0.2 and 0.2-0.3 m) in conventional tillage (CT). In reduced tillage (RT) and no-tillage (NT) treatments significant accumulation of Corg and increase of Cmic in the surface layer were found, compared to CT. No significant differences in Corg and Cmic between two growing cycles were determined; however, mostly higher values were obtained in the top layer of NT during the second growing period. Higher bulk density under conservation tillage techniques did not negatively affect soil characteristics and should be taken in consideration for data evaluation as it can alter interpretation of their changes in the soil profile. Crop yields were comparable in CT and RT. Yield decrease in NT was mostly observed for winter wheat and pea. Beneficial effects of RT and NT conserving soil moisture on crop yield were not observed in dry years.", "keywords": ["soil organic carbon", "Plant culture", "0401 agriculture", " forestry", " and fisheries", "triticum aestivum", "04 agricultural and veterinary sciences", "microbial activity", "01 natural sciences", "pisum sativum", "ploughing", "SB1-1110", "0105 earth and related environmental sciences"], "contacts": [{"organization": "H. Kus\u00e1, Gabriela M\u00fchlbachov\u00e1, P. R\u016f\u017eek,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.17221/567/2015-pse"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%2C%20Soil%20and%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.17221/567/2015-pse", "name": "item", "description": "10.17221/567/2015-pse", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.17221/567/2015-pse"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-12-31T00:00:00Z"}}, {"id": "10.17221/9/2008-swr", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:22:06Z", "type": "Journal Article", "created": "2018-02-11", "title": "The Impact Of Windthrow And Fire Disturbances On Selected Soil Properties In The Tatra National Park", "description": ": In November 2004, forest stands in the Tatra National Park (TANAP) were affected by windthrow and in July 2005, the wildfire broke out on a part of the affected area. The objective of this study is to evaluate the impact of the windthrow and fire disturbances on soil microbial activity. Basal and potential soil respiration, N-mineralisation, catalase activity, soil microbial biomass, and cellulase activity were measured in soil samples taken from the A-horizon (depth of 0-10 cm) along 100 m transects established on 4 plots (reference site, burnt, non-extracted, and extracted sites) in October 2006. Some soil microbial characteristics exhibited a high spatial variability, especially microbial biomass and N-mineralisation. Significant differences in soil microbial characteristics (especially basal soil respiration and catalase activity) between plots were found. Generally, the highest microbial activity was revealed on the plot affected by fire. Soil microbial activity was similar on the extracted and non-extracted sites.", "keywords": ["0106 biological sciences", "windthrow", "S", "0401 agriculture", " forestry", " and fisheries", "Agriculture", "04 agricultural and veterinary sciences", "forest soil", "microbial activity", "01 natural sciences", "wildfire", "spruce stands"]}, "links": [{"href": "https://doi.org/10.17221/9/2008-swr"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20and%20Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.17221/9/2008-swr", "name": "item", "description": "10.17221/9/2008-swr", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.17221/9/2008-swr"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-12-31T00:00:00Z"}}, {"id": "10.3390/microorganisms8071093", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:23:39Z", "type": "Journal Article", "created": "2020-07-22", "title": "New insight into antimicrobial compounds from food and marine-sourced Carnobacterium species through phenotype and genome analyses.", "description": "<p>Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid non-ribosomal peptide synthetase-polyketide synthase), RiPP (ribosomally synthesized and post-translationally modified peptide). In parallel, the antimicrobial activity of 260 strains from seafood products was evaluated. Among the 60% of active strains, three genomes were sequenced and submitted to a dereplication process. C. inhibens MIP2551 produced a high amountof H2O2, probably thanks to the presence of four oxidase-encoding genes. C. maltaromaticum EBP3019 and SF668 strains were highly efficient against Listeria monocytogenes. A new extracellular 16 kDa unmodified bacteriocin in the EBP3019 strain and five different bacteriocins in SF668 were highlighted. In this study, the overview of antimicrobial BGC and inhibitory activities of Carnobacterium spp. allowed the prediction of potential innovative natural products that could be relevant for biotechnological applications.</p>", "keywords": ["Carnobacteriumspp", "0301 basic medicine", "2. Zero hunger", "570", "natural product", "antimicrobial activity", "QH301-705.5", "[SDV]Life Sciences [q-bio]", "NRPS", "hydrogen peroxide", "630", "<i>Carnobacterium </i>spp.", "<i>Carnobacterium</i> spp.", "Article", "lactic acid bacteria", "03 medical and health sciences", "bacteriocin", "genome mining", "RiPP", "Biology (General)", "terpene"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/8/7/1093/pdf"}, {"href": "https://www.mdpi.com/2076-2607/8/7/1093/pdf"}, {"href": "https://doi.org/10.3390/microorganisms8071093"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms8071093", "name": "item", "description": "10.3390/microorganisms8071093", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms8071093"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-21T00:00:00Z"}}, {"id": "10.3390/su1020268", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:23:49Z", "type": "Journal Article", "created": "2009-06-05", "description": "<p>The aim of this study was to evaluate microbial activity in soils under conventional and organic agricultural system management regimes. Soil samples were collected from plots under conventional management (CNV), organic management (ORG) and native vegetation (AVN). Soil microbial activity and biomass was significantly greater in ORG compared with CNV. Soil bulk density decreased three years after adoption of organic system. Soil organic carbon (SOC) was higher in the ORG than in the CNV. The soil under organic agricultural system presents higher microbial activity and biomass and lower bulk density than the conventional agricultural system.</p>", "keywords": ["2. Zero hunger", "jel:O13", "jel:Q", "0401 agriculture", " forestry", " and fisheries", "jel:Q0", "04 agricultural and veterinary sciences", "jel:Q2", "jel:Q56", "15. Life on land", "jel:Q3", "jel:Q5", "microbial activity; microbial biomass; soil organic matter; bulk density"], "contacts": [{"organization": "Ademir S.F. Ara\u00fajo, Luiz F.C. Leite, Valdinar B. Santos, Romero F.V. Carneiro,", "roles": ["creator"]}]}, "links": [{"href": "http://www.mdpi.com/2071-1050/1/2/268/pdf"}, {"href": "https://doi.org/10.3390/su1020268"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/su1020268", "name": "item", "description": "10.3390/su1020268", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/su1020268"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-06-04T00:00:00Z"}}, {"id": "10.5061/dryad.5t76p", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-06-27T16:24:17Z", "type": "Dataset", "title": "Data from: High nighttime humidity and dissolved organic carbon content support rapid decomposition of standing litter in a semi-arid landscape", "description": "unspecifiedDataset_Wang et al.  2017The file contains all  the original data including the temperature, relative humidity, litter  mass remaining, litter DOC concentrations and cumulative C  loss.", "keywords": ["nighttime humidity", "13. Climate action", "standing litter", "PLFA", "SOC", "15. Life on land", "DOC", "microbial activity"], "contacts": [{"organization": "Wang, Jing, Liu, Lingli, Wang, Xin, Yang, Sen, Zhang, Beibei, Li, Ping, Qiao, Chunlian, Deng, Meifeng, Liu, Weixing,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.5t76p"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.5t76p", "name": "item", "description": "10.5061/dryad.5t76p", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.5t76p"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-06T00:00:00Z"}}, {"id": "10.5194/bg-22-601-2025", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:24:39Z", "type": "Journal Article", "created": "2025-01-31", "title": "Contrasting seasonal patterns in particle aggregation and dissolved organic matter transformation in a sub-Arctic fjord", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Abstract. Particulate (POM) and dissolved (DOM) organic matter in the ocean are important components of the Earth's biogeochemical cycle. The two are in a constant state of dynamic change as a result of physical and biochemical processes; however, they are mostly treated as two distinct entities, separated operationally by a filter. We studied the seasonal transition of DOM and POM pools and their drivers in a sub-Arctic fjord by means of monthly environmental sampling and by performing experiments at selected time points. For the experiments, surface water (5\u2009m) was either pre-filtered through a GF/F filter (0.7\u2009\u00b5m) or left unfiltered, followed by 36\u2009h incubations. Before and after incubation, samples were collected for dissolved and particulate organic carbon concentrations (DOC, POC), extracellular polymeric substances (EPSs), microbial community (flow cytometry), and molecular composition of DOM (high-performance liquid chromatography coupled to high-resolution mass spectrometry \u2013 HPLC-HRMS). During the biologically productive period, when environmental POC concentrations were high (April, June, September), the filtered water showed an increase in POC concentrations. While POC concentrations increased in September, DOM lability decreased based on changes in the average hydrogen saturation and aromaticity of DOM molecules. In contrast, during the winter period (December and February), when environmental POC concentrations were low, lower concentrations of POC were measured at the end of the experiments compared to at the start. The change in POC concentrations was significantly different between the biologically productive period and the winter period (t test; p&lt;0.05). Simultaneously, the DOM pool became more labile during the incubation period, as indicated by changes in the average hydrogen saturation, aromaticity, and oxygen saturation, with implications for carbon cycling. The change in POC was not directly associated with an antagonistic change in DOC concentrations, highlighting the complexity of organic matter transformations, making the dynamics between POC and DOC difficult to quantify. However, in both periods, bacterial activity and EPS concentrations increased throughout the incubations, showing that bacterial degradation and physical DOM aggregation drive the transformations of POM and DOM in concert but at varying degrees under different environmental conditions.                     </p></article>", "keywords": ["particulate organic carbon", "seasonal variation", "QE1-996.5", "Ecology", "saturation", "aggregation", "surface water", "fjord", "Geology", "biogeochemical cycle", "Milj\u00f6vetenskap", "dissolved organic carbon", "microbial activity", "environmental conditions", "Life", "QH501-531", "microbial community", "Environmental Sciences", "QH540-549.5"], "contacts": [{"organization": "Maria G. Digernes, Yasemin V. Bodur, Mart\u00ed Amargant-Arum\u00ed, Oliver M\u00fcller, Jeffrey A. Hawkes, Stephen G. Kohler, Ulrike Dietrich, Marit Reigstad, Maria L. Paulsen,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5194/bg-22-601-2025"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeosciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/bg-22-601-2025", "name": "item", "description": "10.5194/bg-22-601-2025", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/bg-22-601-2025"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-31T00:00:00Z"}}, {"id": "10.5281/zenodo.14825718", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-06-27T16:26:04Z", "type": "Dataset", "title": "Raw data for the manuscript: Conventional and biodegradable agricultural microplastics affecting soil properties and microbial functions across a European pedoclimatic gradient", "description": "Clay, Silt, Fine sand, Coarse sand, dry matter, bulk density, pH, electric conductivity, Water extractable organic carbon, Water extractable total nitrogen, Water extractable organic nitrogen,\u00a0Ammonium, Nitrate, Phosphate, Soil organic carbon, Soil total nitrogen, Potential ammonium oxidation, Potential ammonification, Basal respiration, Substrate-induced respiration, Remaining mass of green and black tea litter, Ergosterol concentration, Soil aggregation, Bact and fungi Chao, Bact and fungi Shannon, Bact and fungi InvSimpson, CH4, CO2, N2O", "keywords": ["Microbial community composition", "Microbial activity", "Greenhouse gases", "Teabag index", "Agricultural plastics", "eDNA", "Field experiment"], "contacts": [{"organization": "Smidova, Klara, Hofman, Jakub, Velmala, Sannakajsa, Soinne, Helena, Kim, Shin Woong, Tirroniemi, Jyri, Selonen, Salla,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.14825718"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.14825718", "name": "item", "description": "10.5281/zenodo.14825718", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.14825718"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-06T00:00:00Z"}}, {"id": "10.5281/zenodo.15690367", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:26:33Z", "type": "Report", "title": "AGRIFOODPLAST 2023 International Conference on Micro and Nano-Plastics in the Agri-Food chain", "description": "This is a collection of presentations by PAPILLONS community at AGRIFOODPLAST 2023 International Conference on Micro and Nano-Plastics in the Agri-Food chain.\u00a0 News here: https://www.papillons-h2020.eu/events/agrifoodplast-international-conference-on-micro-and-nano-plastics-in-the-agri-food-chain/", "keywords": ["plants", "microplastic", "soil invertebrates", "soil microbial activity", "mesocosm", "agriculture"], "contacts": [{"organization": "van Loon, Sam, van Gestel, Kees,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.15690367"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.15690367", "name": "item", "description": "10.5281/zenodo.15690367", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.15690367"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-06-18T00:00:00Z"}}, {"id": "10.5281/zenodo.5547311", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:27:03Z", "type": "Dataset", "title": "Energetic return on investment determines overall soil microbial activity.", "description": "<strong>Data</strong> and <strong>R codes</strong> used for the manuscript entitled ' <strong>Energetic return on investment determines overall soil microbial activity.'</strong>", "keywords": ["organic carbon", "community composition", "microorganisms", "bioenergetics", "microbial activity", "FT-ICR-MS", "calorimetry", "soil"], "contacts": [{"organization": "Dufour, Louis J.P., Herrmann, Anke M., Leloup, Julie, Przybylski, C\ufffd\ufffddric, Foti, Ludovic, Abbadie, Luc, Nunan, Naoise,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.5547311"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.5547311", "name": "item", "description": "10.5281/zenodo.5547311", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.5547311"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "11250/3212345", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:29:15Z", "type": "Journal Article", "created": "2025-01-31", "title": "Contrasting seasonal patterns in particle aggregation and dissolved organic matter transformation in a sub-Arctic fjord", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Abstract. Particulate (POM) and dissolved (DOM) organic matter in the ocean are important components of the Earth's biogeochemical cycle. The two are in a constant state of dynamic change as a result of physical and biochemical processes; however, they are mostly treated as two distinct entities, separated operationally by a filter. We studied the seasonal transition of DOM and POM pools and their drivers in a sub-Arctic fjord by means of monthly environmental sampling and by performing experiments at selected time points. For the experiments, surface water (5\u2009m) was either pre-filtered through a GF/F filter (0.7\u2009\u00b5m) or left unfiltered, followed by 36\u2009h incubations. Before and after incubation, samples were collected for dissolved and particulate organic carbon concentrations (DOC, POC), extracellular polymeric substances (EPSs), microbial community (flow cytometry), and molecular composition of DOM (high-performance liquid chromatography coupled to high-resolution mass spectrometry \u2013 HPLC-HRMS). During the biologically productive period, when environmental POC concentrations were high (April, June, September), the filtered water showed an increase in POC concentrations. While POC concentrations increased in September, DOM lability decreased based on changes in the average hydrogen saturation and aromaticity of DOM molecules. In contrast, during the winter period (December and February), when environmental POC concentrations were low, lower concentrations of POC were measured at the end of the experiments compared to at the start. The change in POC concentrations was significantly different between the biologically productive period and the winter period (t test; p&lt;0.05). Simultaneously, the DOM pool became more labile during the incubation period, as indicated by changes in the average hydrogen saturation, aromaticity, and oxygen saturation, with implications for carbon cycling. The change in POC was not directly associated with an antagonistic change in DOC concentrations, highlighting the complexity of organic matter transformations, making the dynamics between POC and DOC difficult to quantify. However, in both periods, bacterial activity and EPS concentrations increased throughout the incubations, showing that bacterial degradation and physical DOM aggregation drive the transformations of POM and DOM in concert but at varying degrees under different environmental conditions.</p></article>", "keywords": ["particulate organic carbon", "seasonal variation", "QE1-996.5", "Ecology", "saturation", "aggregation", "surface water", "fjord", "Geology", "biogeochemical cycle", "Milj\u00f6vetenskap", "dissolved organic carbon", "microbial activity", "environmental conditions", "Life", "QH501-531", "microbial community", "Environmental Sciences", "QH540-549.5"]}, "links": [{"href": "https://doi.org/11250/3212345"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeosciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11250/3212345", "name": "item", "description": "11250/3212345", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11250/3212345"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-31T00:00:00Z"}}, {"id": "10261/405692", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:29:00Z", "type": "Journal Article", "created": "2025-10-03", "title": "Comparison of Isothermal Microcalorimetry Versus Respirometry Assays to Evaluate Short- and Long-term Impact of Microplastics on Soil Microbial Activity", "description": "Abstract           <p>Soil microbial activity is a key indicator of soil health, traditionally assessed using methods like enzymatic activity, community-level physiological profiling (CLPP), microbial biomass, and soil respiration. This study introduces isothermal microcalorimetry assessed with the CalScreener\uffe2\uff84\uffa2 system as a novel tool for evaluating soil activity and validates its use in assessing the impact of anthropogenic pollutants like microplastics. Soil samples were exposed to microplastic particles from conventional and biodegradable plastic mulches, one of the main sources of microplastic pollution in agricultural soils. Microbial activity was assessed after short-term (10\uffc2\uffa0days) and long-term (12\uffc2\uffa0months) microplastic exposure using respirometry, isothermal microcalorimetry, and microbial CLPP. Isothermal microcalorimetry's effectiveness to assess microbial activity was validated by observing distinct thermograms between active and autoclaved soil, and differences between treatments under basal and glucose-amended conditions. Both respirometry and microcalorimetry showed similar results, revealing higher basal activity in soils with biodegradable microplastics after long-term exposure, compared to conventional microplastics and unamended soil. Isothermal microcalorimetry offers advantages over traditional methods, including shorter assessment periods and the need for smaller soil sample amounts. While CLPP did not detect significant differences in overall soil activity among treatments, it may be a useful technique for characterizing microbial functional traits. This study provides, for the first time, insights into the use of isothermal microcalorimetry as a novel methodological approach to evaluate the potential impact of microplastics on soil biological activity.</p", "keywords": ["Microbial activity", "Microcalorimetry", "Microplastics", "Soil health", "Respirometry", "Environmental pollution"], "contacts": [{"organization": "Giovana P. F. Macan, Guillermo Le\u00f3n-Ropero, Juan A. Navas-Cort\u00e9s, Blanca B. Landa,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10261/405692"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%2C%20Air%2C%20%26amp%3B%20Soil%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/405692", "name": "item", "description": "10261/405692", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/405692"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-10-03T00:00:00Z"}}, {"id": "10261/407328", "type": "Feature", "geometry": null, "properties": {"license": "Embargo", "updated": "2026-06-27T16:29:01Z", "type": "Dataset", "title": "HARVEST Project Dataset-France Apple Orchard Soil biological and chemical parameters (2019\u20132020) [Dataset]", "description": "Embargo[ES] Este conjunto de datos incluye informaci\u00f3n qu\u00edmica y biol\u00f3gica del suelo recolectada en un campo de manzanos manejado bajo dos sistemas (Ecol\u00f3gico y Manejo Integrado de Plagas) y tres modalidades de cubierta en las calles (Control, Cubierta de gram\u00edneas, Cubierta de gram\u00edneas y leguminosas). Las mediciones incluyen biomasa microbiana del suelo, propiedades qu\u00edmicas del suelo, colonizaci\u00f3n por hongos micorr\u00edcicos arbusculares (AMF), perfiles catab\u00f3licos microbianos del suelo y la estructura y taxonom\u00eda de la comunidad f\u00fangica del suelo, evaluadas en tres momentos (T0, T1 y T2).", "keywords": ["soil chemistry", "soil health", "soil microorganisms", "Soil microbial biomass", "Soil microbial activity", "Arbuscular mycorrhizal fungi", "Soil microbial catabolic profile", "arbuscular mycorrhizal fungi", "soil microbial biomass", "soil fungal communicity structure and taxonomy", "soil microbial catabolic profile", "Soil health", "soil fungi", "mycorrhizae", "soil microbial activity", "Soil fungal communicity structure and taxonomy"], "contacts": [{"organization": "Mart\u00ednez-Garc\u00eda, Laura B., Creamer, Rachel, Allaphilippe, Aude,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10261/407328"}, {"rel": "self", "type": "application/geo+json", "title": "10261/407328", "name": "item", "description": "10261/407328", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/407328"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-01T00:00:00Z"}}, {"id": "10400.14/37827", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-27T16:29:02Z", "type": "Journal Article", "created": "2022-05-10", "title": "Short-Term Responses of Soil Microbial Communities to Changes in Air Temperature, Soil Moisture and UV Radiation", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>We analyzed the effects on a soil microbial community of short-term alterations in air temperature, soil moisture and ultraviolet radiation and assessed the role of invertebrates (species Enchytraeus crypticus) in modulating the community\u2019s response to these factors. The reference soil, Lufa 2.2, was incubated for 48 h, with and without invertebrates, under the following conditions: standard (20 \u00b0C + 50% water holding capacity (WHC)); increased air temperature (15\u201325 \u00b0C or 20\u201330 \u00b0C + 50% WHC); flood (20 \u00b0C + 75% WHC); drought (20 \u00b0C + 25% WHC); and ultraviolet radiation (UV) (20 \u00b0C + 50% WHC + UV). BIOLOG EcoPlates and 16S rDNA sequencing (Illumina) were used to assess the microbial community\u2019s physiological profile and the bacterial community\u2019s structure, respectively. The bacterial abundance (estimated by 16S rDNA qPCR) did not change. Most of the conditions led to an increase in microbial activity and a decrease in diversity. The structure of the bacterial community was particularly affected by higher air temperatures (20\u201330 \u00b0C, without E. crypticus) and floods (with E. crypticus). Effects were observed at the class, genera and OTU levels. The presence of invertebrates mostly resulted in the attenuation of the observed effects, highlighting the importance of considering microbiome\u2013invertebrate interactions. Considering future climate changes, the effects described here raise concern. This study provides fundamental knowledge to develop effective strategies to mitigate these negative outcomes. However, long-term studies integrating biotic and abiotic factors are needed.</p></article>", "keywords": ["0301 basic medicine", "Soil invertebrates", "Ultraviolet Rays", "drought", "microbial activity", "DNA", " Ribosomal", "Flood", "Article", "Quantitative PCR", "Soil", "03 medical and health sciences", "soil microbiome", "2. Zero hunger", "metagenomics", "increased temperature; drought; flood; UV exposure; microbial activity; bacterial diversity; metagenomics; quantitative PCR; soil microbiome; soil invertebrates", "Soil microbiome", "0303 health sciences", "Drought", "Bacteria", "Microbiota", "bacterial diversity", "Temperature", "Water", "flood", "15. Life on land", "soil invertebrates", "6. Clean water", "UV exposure", "Microbial activity", "Bacterial diversity", "13. Climate action", "quantitative PCR", "Metagenomics", "Increased temperature", "increased temperature"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/13/5/850/pdf"}, {"href": "https://doi.org/10400.14/37827"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10400.14/37827", "name": "item", "description": "10400.14/37827", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10400.14/37827"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-05-10T00:00:00Z"}}, {"id": "11353/10.2037573", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-27T16:29:16Z", "type": "Journal Article", "created": "2023-06-29", "title": "Gold\u2010FISH enables targeted NanoSIMS analysis of plant\u2010associated bacteria", "description": "Summary<p> <p>Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of in\uffc2\uffa0situ evidence of bacterial activity.</p> <p>To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold\uffe2\uff80\uff90based in\uffc2\uffa0situ hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity.</p> <p>We incubated Kosakonia strain DS\uffe2\uff80\uff901\uffe2\uff80\uff90associated, gnotobiotically grown rice plants with 15N\uffe2\uff80\uff93N2 gas to detect in\uffc2\uffa0situ N2 fixation activity. Bacterial cells along the rhizoplane showed\uffc2\uffa0heterogeneous patterns of 15N enrichment, ranging from the natural isotope abundance levels up to 12.07 at% 15N (average and median of 3.36 and 2.85 at% 15N, respectively, n\uffe2\uff80\uff89=\uffe2\uff80\uff89697 cells).</p> <p>The presented correlative optical and chemical imaging analysis is applicable to a broad range of studies investigating plant\uffe2\uff80\uff93microbe interactions. For example, it enables verification of the in\uffc2\uffa0situ metabolic activity of host\uffe2\uff80\uff90associated commercialized strains or plant growth\uffe2\uff80\uff90promoting bacteria, thereby disentangling their role in plant nutrition. Such data facilitate the design of plant\uffe2\uff80\uff93microbe combinations for improvement of crop management.</p> </p", "keywords": ["2. Zero hunger", "106022 Mikrobiologie", "rhizosphere bacteria", "Bacteria", "plant growth-promoting bacteria", "plant\u2013microbe interaction", "Research", "Oryza", "biological nitrogen fixation", "Plants", "microbial activity", "in\u00a0situ hybridization", "Plant Roots", "106026 \u00d6kosystemforschung", "Rhizosphere", "106022 Microbiology", "NanoSIMS", "in situ hybridization", "106026 Ecosystem research", "In situ hybridization", "In Situ Hybridization", "Soil Microbiology", "plant-microbe interaction"]}, "links": [{"href": "https://doi.org/11353/10.2037573"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11353/10.2037573", "name": "item", "description": "11353/10.2037573", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11353/10.2037573"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-06-28T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=microbial+activity&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=microbial+activity&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=microbial+activity&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=microbial+activity&offset=44", "hreflang": "en-US"}], "numberMatched": 44, "numberReturned": 44, "distributedFeatures": [], "timeStamp": "2026-06-27T22:25:09.520201Z"}