{"type": "FeatureCollection", "features": [{"id": "10.5281/zenodo.8109600", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:22:58Z", "type": "Dataset", "title": "Data on soil compounds, respiration and incorporation of 13C-labeled substrate", "description": "Open AccessSee Readme.pdf", "keywords": ["2. Zero hunger", "microdialysis", "respiration rates", "compound concentration in soil solution", "PLFA and NLFA", "13C isotopic labeling", "15. Life on land", "6. 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This creates \u2018hotspots\u2019 of microbial activity characterized by accelerated soil organic matter turnover with direct implications for nutrient availability for plants. However, our knowledge of the microbial metabolic processes occurring in the immediate vicinity of roots during and after a root exudation event is still limited.<br/><br/>Using reverse microdialysis, we simulated root exudation by releasing a13C-labelled mix of low-molecular-weight organic C compounds at mm-sized locations in undisturbed soil. Combined with stable isotope tracing, we investigated the fine-scale temporal and spatial response of microbial metabolism, soil chemistry, and traced microbial respiration and uptake of exuded compounds.<br/><br/>Our results show that a 9-h simulated root exudation pulse leads to i) a large local respiration event and ii) alteration of the temporal dynamics of soil metabolites over the following 12\u202fday\u202fat the exudation spot. Notably, we observed a threefold increase in ammonium concentrations at 12\u202fh and increased nitrate concentrations five days after the pulse. Moreover, various short-chain fatty acids (acetate, propionate, formate) increased over the following days, indicating altered microbial metabolic pathways and activity. Phospholipid and neutral lipid fatty acids (PLFAs, NLFAs) of all major microbial groups were significantly 13C-enriched within a 5\u202fmm radius around the microdialysis probes, but not beyond. The highest relative 13C enrichment was observed in fungal NLFAs, indicating that a significant proportion of the exuded compounds had been incorporated into fungal storage compounds.<br/><br/>Our findings indicate that the punctual release of low-molecular-weight organic C compounds into intact soil significantly changes microbial metabolism and activity in its immediate surroundings, enhancing mineralization of native organic nitrogen. This highlights the versatility of microbial metabolic pathways in response to rapidly changing C availability and their effectiveness in increasing nutrient availability near plant roots.", "keywords": ["Oxygen depletion", "2. Zero hunger", "570", "106022 Mikrobiologie", "Root exudation", "short-chain fatty acids", "Reverse microdialysis", "reverse microdialysis", "[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study", "15. Life on land", "root exudation", "6. 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We utilized three different artificial root exudates: sugars (glucose, fructose), organic acids (acetate, succinate), and a combination of sugars and organic acids (glucose, fructose, acetate, succinate); alongside a water-only control for comparison.  We collected compounds from soil solutions and measured respiration. Due to 13C-labeled substrate we could differentiate between substrate-derived respiration and SOM-derived respiration. Additionally, we extracted lipid fatty acids from soil and measured their 13C incorporation.", "keywords": ["microdialysis", "respiration rates", "NLFA", "PLFA", "13C isotopic labeling", "soil compounds"], "contacts": [{"organization": "Wiesenbauer, Julia, Kaiser, Christina,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.13338423"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.13338423", "name": "item", "description": "10.5281/zenodo.13338423", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.13338423"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-18T00:00:00Z"}}, {"id": "10.5281/zenodo.8109601", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:22:58Z", "type": "Dataset", "title": "Data on soil compounds, respiration and incorporation of 13C-labeled substrate", "description": "Open AccessSee Readme.pdf", "keywords": ["2. 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