{"type": "FeatureCollection", "features": [{"id": "10.1007/s10646-009-0329-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:35Z", "type": "Journal Article", "created": "2009-06-06", "title": "Trace Elements And Activity Of Antioxidative Enzymes In Cistus Ladanifer L. Growing On An Abandoned Mine Area", "description": "The Mediterranean shrub Cistus ladanifer grows naturally in S\u00e3o Domingos (Portugal), an abandoned copper mine. High levels of trace elements in plants can generate oxidative stress increasing the activity of antioxidant enzymes. The aim of this work was to evaluate and compare As, Cu, Pb and Zn concentrations and the activity of the soluble and cell wall ionically bounded forms of the enzymes catalase, peroxidase and superoxide dismutase in leaves of C. ladanifer, collected in spring and summer, growing on S\u00e3o Domingos mine and on a non-contaminated area (Pomar\u00e3o). S\u00e3o Domingos soils showed high total concentrations of As (2.6 g kg(-1)) and Pb (7.3 g kg(-1)) however the available fraction represented less than 1.5% of the total. C. ladanifer population from mine showed tolerance to Pb and Zn, which attain in leaves concentrations considered toxic for plants. The enzymatic activity of catalase, peroxidise and superoxide dismutase varied with plant populations and seasons, although with no particular trend, being specific to each trace element and enzyme cell localization. Catalase activity was evenly distributed between the soluble and ionically bounded forms, whereas the ionically bounded form of peroxidase predominated relatively to total activity, and the opposite was observed for superoxide dismutase. Spring and summer leaves from the two areas presented enzymatic activities in both fractions except to peroxidase soluble activities in leaves collected in summer. C. ladanifer enzymatic activity seems to be related with the co-existence of different stress factors (trace elements concentration, temperature, UV radiation and drought). The survival and growth of this species on contaminated mining soils is due to the presence of effective antioxidant enzyme-based defence systems.", "keywords": ["0106 biological sciences", "trace elements", "Industrial Waste", "antioxidative enzymes", "Antioxidative enzymes", "01 natural sciences", "Mining", "Soil", "Cell Wall", "Soil Pollutants", "adaptative capacity", "0105 earth and related environmental sciences", "Trace elements", "Portugal", "Cistus", "Adaptative capacity", "15. Life on land", "Sulphide abandoned mine", "cistus ladanifer L.", "Trace Elements", "Plant Leaves", "Oxidative Stress", "sulphide abandoned mine", "Cistus ladanifer L.", "Oxidoreductases", "Copper"]}, "links": [{"href": "https://doi.org/10.1007/s10646-009-0329-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecotoxicology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10646-009-0329-y", "name": "item", "description": "10.1007/s10646-009-0329-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10646-009-0329-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-06-05T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.174667", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:16:03Z", "type": "Journal Article", "created": "2024-07-10", "title": "Reproduction, growth and oxidative stress in earthworm Eisenia andrei exposed to conventional and biodegradable mulching film microplastics", "description": "Plastic contamination in agricultural soils has become increasingly evident. Plastic mulching films are widely used in agricultural practices. However, the increased use of biodegradable plastics has, to some extent, replaced their non-degradable counterparts. The fragmentation of plastics generates microplastics (MPs), posing risk to soil functions and organisms. In this study the effects of low-density polyethylene microplastics (PE-MP) and polybutylene adipate terephthalate biodegradable microplastics (PBAT-BD-MP) originating from mulching films on the earthworm Eisenia andrei were studied. The earthworms were exposed to seven concentrations (0, 0.005, 0.05, 0.1, 0.5, 1, and 5\u00a0% w/w) based on environmentally relevant levels and worst-case scenarios on soil contamination. Survival, growth, reproduction, and biomarkers for oxidative stress [superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST), glutathione (GSH), and lipid peroxidation (LPO)] were analysed. Additionally, the Integrated Biomarker Response Index (IBR) was calculated to assess the overall oxidative stress status of the earthworms. Results showed that PE-MP exposure slightly decreased the biomass of the earthworms towards higher concentrations, whereas PBAT-BD-MPs induced growth at lower concentrations. MPs did not have a significant effect on Eisenia andrei reproduction; however, a slight negative trend was observed in juvenile production with increasing PE-MP concentrations. Both PE-MP and PBAT-BD-MP affected antioxidant system, PE-MPs with changes in CAT and GR levels and PBAT-BD-MPs inducing effects on SOD and LPO levels. Additionally, both MPs exhibited effects on soil parameters, resulting in increased soil pH and water-holding capacity at 5\u00a0% concentration. Changes in soil parameters can further affect soil organisms such as earthworms. This study provides understanding of the ecotoxicological effects of conventional and biodegradable microplastics on the earthworm Eisenia andrei. It also shows that MP particles of both conventional and biodegradable mulching films induce oxidative stress, considered as an early-warning indicator for adverse ecological effects, in environmentally relevant concentrations.", "keywords": ["lierot", "soil ecotoxicology", "LDPE", "Microplastics", "School of Resource Wisdom", "maaper\u00e4biologia", "Biodegradable Plastics", "Resurssiviisausyhteis\u00f6", "maatalous", "Animals", "Soil Pollutants", "PBAT", "Oligochaeta", "oksidatiivinen stressi", "Glutathione Transferase", "mikromuovi", "2. Zero hunger", "maaper\u00e4", "agricultural soil", "Superoxide Dismutase", "Reproduction", "biodegradable plastic", "Catalase", "ymp\u00e4rist\u00f6kuormitus", "biohajoaminen", "environmental stress", "ekotoksikologia", "Oxidative Stress", "maaper\u00e4el\u00e4imist\u00f6", "muovi", "Polyethylene", "13. Climate action", "Lipid Peroxidation", "Biomarkers"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2024.174667"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.174667", "name": "item", "description": "10.1016/j.scitotenv.2024.174667", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.174667"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2024.125307", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:30Z", "type": "Journal Article", "created": "2024-11-12", "title": "Biodegradable microplastics induce profound changes in lettuce (Lactuca sativa) defense mechanisms and to some extent deteriorate growth traits", "description": "The development of agricultural technologies has intensified the use of plastic in this sector. Products of plastic degradation, such as microplastics (MPs), potentially threaten living organisms, biodiversity and agricultural ecosystem functioning. Thus, biodegradable plastic materials have been introduced to agriculture. However, the effects of biodegradable plastic substitutes on soil ecosystems are even less known than those of traditional ones. Here, we studied the effects of environmentally relevant concentrations of MPs prepared from a biodegradable plastic (a starch-polybutylene adipate terephthalate blend, PBAT-BD-MPs) on the growth and defense mechanisms of lettuce (Lactuca sativa) in CLIMECS system (CLImatic Manipulation of ECosystem Samples). PBAT-BD-MPs in the highest concentrations negatively affected some traits of growth, i.e., dry weight percentage, specific leaf area, and both C and N contents. We observed more profound changes in plant physiology and biochemistry, as PBAT-BD-MPs decreased chlorophyll content and triggered a concerted response of plant defense mechanisms against oxidative stress. In conclusion, exposure to PBAT-BD-MPs induced plant oxidative stress and activated plant defense mechanisms, leading to oxidative homeostasis that sustained plant growth and functioning. Our study highlights the need for in-depth understanding of the effect of bioplastics on plants.", "keywords": ["580", "Chlorophyll", "0301 basic medicine", "570", "0303 health sciences", "salicylic acid", "Microplastics", "Lipid peroxidation", "lipid peroxidation", "Salicylic acid", "Biodegradable Plastics", "Plant Leaves", "Oxidative Stress", "03 medical and health sciences", "Starch-polybutylene adipate terephthalate", "Biodegradation", " Environmental", "total phenolic content", "starch-polybutylene adipate terephthalate", "Soil Pollutants", "PBAT", "Total phenolic content", "CLIMECS system", "Lactuca"], "contacts": [{"organization": "Sylwia Adamczyk, Laura J. Zantis, Sam van Loon, Cornelis A.M. van Gestel, Thijs Bosker, Rachel Hurley, Luca Nizzetto, Bartosz Adamczyk, Sannakajsa Velmala,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2024.125307"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2024.125307", "name": "item", "description": "10.1016/j.envpol.2024.125307", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2024.125307"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.1007/s11356-014-3661-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:49Z", "type": "Journal Article", "created": "2014-10-13", "title": "Effects Of Ambient And Elevated Co2 On Growth, Chlorophyll Fluorescence, Photosynthetic Pigments, Antioxidants, And Secondary Metabolites Of Catharanthus Roseus (L.) G Don. Grown Under Three Different Soil N Levels", "description": "Catharanthus roseus L. plants were grown under ambient (375\u2009\u00b1\u200930 ppm) and elevated (560\u2009\u00b1\u200925 ppm) concentrations of atmospheric CO2 at different rates of N supply (without supplemental N, 0 kg N ha(-1); recommended N, 50 kg N ha(-1); and double recommended N, 100 kg N ha(-1)) in open top chambers under field condition. Elevated CO2 significantly increased photosynthetic pigments, photosynthetic efficiency, and organic carbon content in leaves at recommended (RN) and double recommended N (DRN), while significantly decreased total nitrogen content in without supplemental N (WSN). Activities of superoxide dismutase, catalase, and ascorbate peroxidase were declined, while glutathione reductase, peroxidase, and phenylalanine-ammonia lyase were stimulated under elevated CO2. However, the responses of the above enzymes were modified with different rates of N supply. Elevated CO2 significantly reduced superoxide production rate, hydrogen peroxide, and malondialdehyde contents in RN and DRN. Compared with ambient, total alkaloids content increased maximally at recommended level of N, while total phenolics in WSN under elevated CO2. Elevated CO2 stimulated growth of plants by increasing plant height and numbers of branches and leaves, and the magnitude of increment were maximum in DRN. The study suggests that elevated CO2 has positively affected plants by increasing growth and alkaloids production and reducing the level of oxidative stress. However, the positive effects of elevated CO2 were comparatively lesser in plants grown under limited N availability than in moderate and higher N availability. Furthermore, the excess N supply in DRN has stimulated the growth but not the alkaloids production under elevated CO2.", "keywords": ["Chlorophyll", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Catharanthus", "Nitrogen", "Secondary Metabolism", "Hydrogen Peroxide", "Carbon Dioxide", "Plants", "15. Life on land", "Antioxidants", "Fluorescence", "6. Clean water", "3. Good health", "Plant Leaves", "Oxidative Stress", "Soil", "03 medical and health sciences", "Superoxides", "Malondialdehyde", "Photosynthesis"], "contacts": [{"organization": "Madhoolika Agrawal, Aradhana Singh,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s11356-014-3661-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-014-3661-6", "name": "item", "description": "10.1007/s11356-014-3661-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-014-3661-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-10-12T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2024.143131", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:20Z", "type": "Journal Article", "created": "2024-08-19", "title": "Photoaging of polystyrene-based microplastics amplifies inflammatory response in macrophages", "description": "The continuous release of municipal and industrial products into the environment poses a growing concern for public health. Among environmental pollutants, polystyrene (PS) stands out as a primary constituent of environmental plastic waste, given its widespread use and high production rates owing to its durability and user-friendly properties. The detection of polystyrene microparticles (PS-MPs) in various living organisms has been well-documented, posing a serious threat due to their potential passage into the human ecosystem. In this manuscript, we aimed to study the toxicological effects of low concentrations of pristine and photoaged PS-MPs in a murine macrophage cell line. To this purpose, PS-MPs were photoaged by indoor exposure to visible light to simulate environmental weathering due to solar irradiation (PS-MPs3h). Physical characterization revealed that the irradiation treatment results in particle degradation and the possible release of nanoparticles. Monocultures of the RAW264.7\u00a0cell line were then exposed to PS-MPs and PS-MPs3h at concentrations comparable to experimental measurements from biological samples, to assess cytotoxicity, intracellular oxidative stress, primary genotoxicity, and inflammatory effects. Significant toxicity-related outcomes were observed in cells treated with both pristine PS-MPs and PS-MPs3h even at low concentrations (0,10\u00a0\u03bcg/ml and 1\u00a0\u03bcg/ml). PS-MPs3h exhibited greater adverse effects compared to PS-MPs, including reduced cell viability, increased ROS production, elevated DNA damage, and upregulation of IL-6 and NOS2 gene expression. Therefore, we can conclude that changes induced by environmental aging in the physicochemical composition of PS microplastics play a crucial role in the adverse health outcomes associated with microplastic exposure.", "keywords": ["Inflammation", "Mice", "Oxidative Stress", "RAW 264.7 Cells", "microplastics", " polystyrene", " macrophages", "Cell Survival", "Microplastics", "Macrophages", "Polystyrenes", "Animals", "Environmental Pollutants", "Reactive Oxygen Species", "DNA Damage"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/493702/1/Aloi%20et%20al%20Chemospere%202024.pdf"}, {"href": "https://doi.org/10.1016/j.chemosphere.2024.143131"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2024.143131", "name": "item", "description": "10.1016/j.chemosphere.2024.143131", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2024.143131"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2006.12.071", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:19Z", "type": "Journal Article", "created": "2007-02-21", "title": "The Copper Tolerance Strategies And The Role Of Antioxidative Enzymes In Three Plant Species Grown On Copper Mine", "description": "This study was undertaken to identify the strategies and the status of antioxidant enzyme activities involved in three plant species tolerance against Cu-toxicity in copper mine. The following methods were used for evaluations in three wild type species; Datura stramonium, Malva sylvestris and Chenopodium ambrosioides. The level of chlorophyll and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) by spectrometry, malondialdehyde (MDA) and dityrosine by HPLC and the levels of Cu in tissues and soils by atomic absorption spectrometry (AAS). Analysis showed that total and available copper were at toxic levels for plants growing on contaminated soil (zone 1). However, there were not any visual and conspicuous symptoms of Cu toxicity in plant species. Among three species, excess copper was transferred only into the D. stramonium and C. ambrosioides tissues. The C. ambrosioides accumulated Cu in roots and then in leaves, in which the leaves chloroplasts stored Cu around two times of vacuoles. In D. stramonium most of Cu was accumulated in leaves in which the storage rate in vacuoles and chloroplasts were 42% and 8%, respectively. In zone 1, the chlorophyll levels increased significantly in leaves of C. ambrosioides with respect to the same plant growing on uncontaminated soil (zone 2). There was insignificant decrease in chlorophyll content of D. stramonium leaves, collected from zone 1 with respect to zone 2. The D. stramonium and C. ambrosioides in zone 1, both revealed significant increase in their tissues antioxidant enzyme activities in comparison with the same samples of zone 2. There was significant elevation in oxidative damage biomarkers; MDA and dityrosine, when the aerial parts of D. stramonium in zone 1 were compared with the same parts of zone 2. We concluded that there were different tolerance strategies in studied plant species that protected them against copper toxicity. In M. sylvestris, exclusion of Cu from the roots or its stabilization in the soil restricted Cu toxicity effects. On the other hand D. stramonium and C. ambrosioides, elevated their antioxidative enzyme activities in response to cu-toxicity. In addition, the species D. stramonium accumulated excess of Cu in leaves vacuoles.", "keywords": ["Chlorophyll", "0106 biological sciences", "0301 basic medicine", "Chloroplasts", "Malva", "Plants", "15. Life on land", "Lipid Metabolism", "Plant Roots", "01 natural sciences", "Antioxidants", "Mining", "6. Clean water", "Chenopodium", "Oxidative Stress", "Soil", "03 medical and health sciences", "Malondialdehyde", "Vacuoles", "Datura", "Tyrosine", "Biomass", "Copper", "Plant Proteins"], "contacts": [{"organization": "Massod Mashhadi Akbar Boojar, Faranak Goodarzi,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2006.12.071"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2006.12.071", "name": "item", "description": "10.1016/j.chemosphere.2006.12.071", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2006.12.071"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-05-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2024.143519", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:20Z", "type": "Journal Article", "created": "2024-10-10", "title": "Evaluation of per- and polyfluoroalkyl substances (PFAS) toxic effects on the acute inflammatory response in the medicinal leech Hirudo verbana", "description": "Per- and polyfluoroalkyl (PFAS) substances are a large group of chemicals with elevated water and oil-resistance properties, widely implicated in various applicative fields. Due to the extensive use and high resistance to degradative factors, these compounds pose a significant risk of environmental spreading, bioaccumulating also in living organisms. In this context, despite many researches have been performed to demonstrate 'legacy' PFAS harmfulness, only few data are still available about all the emerging fluorinated molecules, industrially introduced to replace the previous ones. For this reason, we proposed the medicinal leech Hirudo verbana as consolidated invertebrate model to assess the effects of four different PFAS (HFPO-DA, PFMoBa, PFOA and PFMOPrA) following freshwater dispersion. Morphological, immunohistochemical and molecular analyses demonstrate that, despite all the compounds basically induce an acute inflammatory and oxidative stress response, a different cellular and molecular response has been observed. Whereas for PFOA and PFMOPrA an increase in the tested concentration leads to a corresponding rise in the immune response, HFPO-DA and PFMoBa trigger an entirely opposite effect. Indeed, the significant recruitment of both granulocytes and macrophage like cells, typically involved in the removal of non-self, is inhibited with increasing concentrations of these compounds. The data collected revealed a different sensitivity of the leech immune system following PFAS exposure, requiring to deepen the current knowledge on the potential toxicity of these compounds.", "keywords": ["Inflammation", "Fluorocarbons", "PFAS", "Freshwater environment", "Inflammatory response", "610", "Toxicology", "Hirudo medicinalis", "Oxidative Stress", "Leeches", "628", "Oxidative stre", "Animals", "Medicinal leech", "Water Pollutants", " Chemical"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2024.143519"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2024.143519", "name": "item", "description": "10.1016/j.chemosphere.2024.143519", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2024.143519"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2006.10.013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:28Z", "type": "Journal Article", "created": "2006-12-01", "title": "Behavior Of Trifolium Repens And Lolium Perenne Growing In A Heavy Metal Contaminated Field: Plant Metal Concentration And Phytotoxicity", "description": "The use of a vegetation cover for the management of heavy metal contaminated soils needs prior investigations on the plant species the best sustainable. In this work, behaviors of Trifolium repens and Lolium perenne, growing in a metal-polluted field located near a closed lead smelter, were investigated through Cd, Pb and Zn-plant metal concentrations and their phytotoxicity. In these plant species, metals were preferentially accumulated in roots than in shoots, as follow: Cd>Zn>Pb. Plant exposure to such metals induced oxidative stress in the considered organs as revealed by the variations in malondialdehyde levels and superoxide dismutase activities. These oxidative changes were closely related to metal levels, plant species and organs. Accordingly, L. perenne seemed to be more affected by metal-induced oxidative stress than T. repens. Taken together, these findings allow us to conclude that both the plant species could be suitable for the phytomanagement of metal-polluted soils.", "keywords": ["Superoxide Dismutase", "Environmental Exposure", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "01 natural sciences", "Oxidative Stress", "Soil", "Zinc", "Biodegradation", " Environmental", "Lead", "Malondialdehyde", "Metals", " Heavy", "Lolium", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Trifolium", "Plant Shoots", "Cadmium", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2006.10.013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2006.10.013", "name": "item", "description": "10.1016/j.envpol.2006.10.013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2006.10.013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-06-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2017.10.007", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:16:26Z", "type": "Journal Article", "created": "2017-10-27", "title": "The role of operating parameters and oxidative damage mechanisms of advanced chemical oxidation processes in the combat against antibiotic-resistant bacteria and resistance genes present in urban wastewater", "description": "An upsurge in the study of antibiotic resistance in the environment has been observed in the last decade. Nowadays, it is becoming increasingly clear that urban wastewater is a key source of antibiotic resistance determinants, i.e. antibiotic-resistant bacteria and antibiotic resistance genes (ARB&ARGs). Urban wastewater reuse has arisen as an important component of water resources management in the European Union and worldwide to address prolonged water scarcity issues. Especially, biological wastewater treatment processes (i.e. conventional activated sludge), which are widely applied in urban wastewater treatment plants, have been shown to provide an ideal environment for the evolution and spread of antibiotic resistance. The ability of advanced chemical oxidation processes (AOPs), e.g. light-driven oxidation in the presence of H2O2, ozonation, homogeneous and heterogeneous photocatalysis, to inactivate ARB and remove ARGs in wastewater effluents has not been yet evaluated through a systematic and integrated approach. Consequently, this review seeks to provide an extensive and critical appraisal on the assessment of the efficiency of these processes in inactivating ARB and removing ARGs in wastewater effluents, based on recent available scientific literature. It tries to elucidate how the key operating conditions may affect the process efficiency, while pinpointing potential areas for further research and major knowledge gaps which need to be addressed. Also, this review aims at shedding light on the main oxidative damage pathways involved in the inactivation of ARB and removal of ARGs by these processes. In general, the lack and/or heterogeneity of the available scientific data, as well as the different methodological approaches applied in the various studies, make difficult the accurate evaluation of the efficiency of the processes applied. Besides the operating conditions, the variable behavior observed by the various examined genetic constituents of the microbial community, may be directed by the process distinct oxidative damage mechanisms in place during the application of each treatment technology. For example, it was shown in various studies that the majority of cellular damage by advanced chemical oxidation may be on cell wall and membrane structures of the targeted bacteria, leaving the internal components of the cells relatively intact/able to repair damage. As a result, further in-depth mechanistic studies are required, to establish the optimum operating conditions under which oxidative mechanisms target internal cell components such as genetic material and ribosomal structures more intensively, thus conferring permanent damage and/or death and preventing potential post-treatment re-growth.", "keywords": ["Titanium", "Photolysis", "Bacteria", "Sewage", "Sulfates", "Ultraviolet Rays", "0211 other engineering and technologies", "Drug Resistance", " Microbial", "Hydrogen Peroxide", "02 engineering and technology", "Wastewater", "Oxidants", "01 natural sciences", "6. Clean water", "Water Purification", "12. Responsible consumption", "Oxidative Stress", "Ozone", "Genes", " Bacterial", "13. Climate action", "Antibiotic resistance Advanced chemical oxidation Inactivation mechanisms Wastewater treatment", "Drug Resistance", " Bacterial", "11. Sustainability", "Oxidation-Reduction", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2017.10.007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2017.10.007", "name": "item", "description": "10.1016/j.watres.2017.10.007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2017.10.007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-01T00:00:00Z"}}, {"id": "10.1093/femsec/fiae152", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:17:14Z", "type": "Journal Article", "created": "2024-11-19", "title": "A respiro-fermentative strategy to survive nanoxia in Acidobacterium capsulatum", "description": "Abstract                <p>Microbial soil habitats are characterized by rapid shifts in substrate and nutrient availabilities, as well as chemical and physical parameters. One such parameter that can vary in soil is oxygen; thus, microbial survival is dependent on adaptation to this substrate. To better understand the metabolic abilities and adaptive strategies to oxygen-deprived environments, we combined genomics with transcriptomics of a model organism, Acidobacterium capsulatum, to explore the effect of decreasing, environmentally relevant oxygen concentrations. The decrease from 10 to 0.1\uffc2\uffa0\uffc2\uffb5M oxygen (3.6 to 0.036 pO2% present atmospheric level, respectively) caused the upregulation of the transcription of genes involved in signal transduction mechanisms, energy production and conversion and secondary metabolites biosynthesis, transport, and catabolism based on clusters of orthologous group categories. Contrary to established observations for aerobic metabolism, key genes in oxidative stress response were significantly upregulated at lower oxygen concentrations, presumably due to an NADH/NAD+ redox ratio imbalance as the cells transitioned into nanoxia. Furthermore, A. capsulatum adapted to nanoxia by inducing a respiro-fermentative metabolism and rerouting fluxes of its central carbon and energy pathways to adapt to high NADH/NAD+ redox ratios. Our results reveal physiological features and metabolic capabilities that allowed A. capsulatum to adapt to oxygen-limited conditions, which could expand into other environmentally relevant soil strains.</p", "keywords": ["0301 basic medicine", "106022 Mikrobiologie", "0303 health sciences", "Acidobacteriota", "NADH imbalances", "microaerobic respiration", "Gene Expression Regulation", " Bacterial", "Adaptation", " Physiological", "oxygen limitation", "Acidobacteria", "Oxygen", "Oxidative Stress", "03 medical and health sciences", "106026 \u00d6kosystemforschung", "Fermentation", "106022 Microbiology", "106026 Ecosystem research", "fermentation", "transcriptome", "Oxidation-Reduction", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/10.1093/femsec/fiae152"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiae152", "name": "item", "description": "10.1093/femsec/fiae152", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiae152"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-11-18T00:00:00Z"}}, {"id": "10.1093/jxb/erq249", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:17:16Z", "type": "Journal Article", "created": "2010-08-27", "title": "Plant Physiology And Proteomics Reveals The Leaf Response To Drought In Alfalfa (Medicago Sativa L.)", "description": "Despite its relevance, protein regulation, metabolic adjustment, and the physiological status of plants under drought is not well understood in relation to the role of nitrogen fixation in nodules. In this study, nodulated alfalfa plants were exposed to drought conditions. The study determined the physiological, metabolic, and proteomic processes involved in photosynthetic inhibition in relation to the decrease in nitrogenase (N(ase)) activity. The deleterious effect of drought on alfalfa performance was targeted towards photosynthesis and N(ase) activity. At the leaf level, photosynthetic inhibition was mainly caused by the inhibition of Rubisco. The proteomic profile and physiological measurements revealed that the reduced carboxylation capacity of droughted plants was related to limitations in Rubisco protein content, activation state, and RuBP regeneration. Drought also decreased amino acid content such as asparagine, and glutamic acid, and Rubisco protein content indicating that N availability limitations were caused by N(ase) activity inhibition. In this context, drought induced the decrease in Rubisco binding protein content at the leaf level and proteases were up-regulated so as to degrade Rubisco protein. This degradation enabled the reallocation of the Rubisco-derived N to the synthesis of amino acids with osmoregulant capacity. Rubisco degradation under drought conditions was induced so as to remobilize Rubisco-derived N to compensate for the decrease in N associated with N(ase) inhibition. Metabolic analyses showed that droughted plants increased amino acid (proline, a major compound involved in osmotic regulation) and soluble sugar (D-pinitol) levels to contribute towards the decrease in osmotic potential (\u03a8(s)). At the nodule level, drought had an inhibitory effect on N(ase) activity. This decrease in N(ase) activity was not induced by substrate shortage, as reflected by an increase in total soluble sugars (TSS) in the nodules. Proline accumulation in the nodule could also be associated with an osmoregulatory response to drought and might function as a protective agent against ROS. In droughted nodules, the decrease in N(2) fixation was caused by an increase in oxygen resistance that was induced in the nodule. This was a mechanism to avoid oxidative damage associated with reduced respiration activity and the consequent increase in oxygen content. This study highlighted that even though drought had a direct effect on leaves, the deleterious effects of drought on nodules also conditioned leaf responsiveness.", "keywords": ["Proteomics", "0301 basic medicine", "570", "Rubisco", "Proteome", "[SDV]Life Sciences [q-bio]", "proteome", "N-2 FIXATION RESPONSE", "drought", "N2 fixation", "03 medical and health sciences", "XANTHOPHYLL CYCLE", "N-2 fixation", "2-CYSTEINE PEROXIREDOXIN", "Nitrogenase", "oxidative stress", "AMINO-ACIDS", "Photosynthesis", "climate", "agriculture", "Plant Proteins", "580", "N remobilization", "2. Zero hunger", "0303 health sciences", "photosynthesis", "PINITOL ACCUMULATION", "Drought", "RIBULOSE-1", "5-BISPHOSPHATE CARBOXYLASE-OXYGENASE", "Water", "BRASSICA-NAPUS", "N(O)-TERT-BUTYLDIMETHYLSILYL DERIVATIVES", "15. Life on land", "Research Papers", "6. Clean water", "Droughts", "[SDV] Life Sciences [q-bio]", "Plant Leaves", "nitrogen fixation", "Oxidative stress", "rubisco", "NITROGEN-FIXATION", "WATER-WATER CYCLE", "Medicago sativa"]}, "links": [{"href": "https://doi.org/10.1093/jxb/erq249"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jxb/erq249", "name": "item", "description": "10.1093/jxb/erq249", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jxb/erq249"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-25T00:00:00Z"}}, {"id": "10.1111/j.1438-8677.2012.00627.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:17:39Z", "type": "Journal Article", "created": "2012-06-05", "title": "Physiological And Biochemical Responses Ofquercus Pubescensto Air Warming And Drought On Acidic And Calcareous Soils", "description": "Abstract<p>The drought\uffe2\uff80\uff90 and thermo\uffe2\uff80\uff90tolerant Quercus pubescens, a tree species growing on both acidic and calcareous soils in the sub\uffe2\uff80\uff90Mediterranean region, was exposed to soil drought (\uffe2\uff88\uff9260% to \uffe2\uff88\uff9280% soil water content) and air warming (+1.2\uffe2\uff80\uff83\uffc2\uffb0C daytime temperature), singly and in combination. The experiment was conducted on two natural forest soils with similar texture but different pH (acidic and calcareous soils). The physiological (photosynthesis) and biochemical (antioxidant system) responses of Q.\uffc2\uffa0pubescens were investigated. On acidic soil, Q.\uffc2\uffa0pubescens had a higher reactive oxygen species (ROS) content than on calcareous soil, confirming that this species is better adapted to the latter soil type. A down\uffe2\uff80\uff90regulation of ascorbate\uffe2\uff80\uff90glutathione cycle enzymes suggests that ROS were used as signalling molecules. Air warming stimulated stomatal opening, while soil drought induced stomatal closure in the late afternoon and reduced Rubisco carboxylation efficiency. Photosynthetic performance in the combined treatment was higher than under single drought stress and similar to control and air warming. Q.\uffc2\uffa0pubescens biochemical responses depended on soil pH. On acidic soil, Q.\uffc2\uffa0pubescens trees exposed to air warming used ROS as signalling molecules. On calcareous soil, these trees were able to balance both soil drought and air warming stress, avoiding ROS toxic effects by increasing antioxidant enzyme activitiy and maintaining a high enzymatic antioxidant defence. When combined, drought and air warming induced either more severe (higher oxidative pressure and impairment of the light\uffe2\uff80\uff90harvesting complex) or different responses (decline of the thermal energy dissipation capacity) relative to the single stressors. Overall, however, Q.\uffc2\uffa0pubescens preserved the functionality of the photosynthetic apparatus and controlled the antioxidant system response, thus confirming its drought and thermo\uffe2\uff80\uff90tolerance and therefore its potential to adapt to the ongoing climate change.</p>", "keywords": ["0106 biological sciences", "0301 basic medicine", "Hot Temperature", "Climate", "Ribulose-Bisphosphate Carboxylase", "Light-Harvesting Protein Complexes", "Down-Regulation", "Global Warming", "01 natural sciences", "Antioxidants", "Quercus", "Soil", "03 medical and health sciences", "Antioxidant enzyme; ascorbate-glutathione cycle; chlorophyll a fluorescence; gas exchange; oak", "Stress", " Physiological", "Photosynthesis", "Ecosystem", "Antioxidant enzyme; ascorbate-glutathione cycle; chlorophyll a fluorescence; gas exchange; oak.", "Air", "Water", "15. Life on land", "Hydrogen-Ion Concentration", "Adaptation", " Physiological", "6. Clean water", "Droughts", "Oxidative Stress", "13. Climate action", "Plant Stomata"]}, "links": [{"href": "https://doi.org/10.1111/j.1438-8677.2012.00627.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1438-8677.2012.00627.x", "name": "item", "description": "10.1111/j.1438-8677.2012.00627.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1438-8677.2012.00627.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-06-05T00:00:00Z"}}, {"id": "10.1371%2fjournal.pone.0044697", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:18:02Z", "type": "Journal Article", "created": "2012-09-11", "title": "A Novel Stress-Induced Sugarcane Gene Confers Tolerance To Drought, Salt And Oxidative Stress In Transgenic Tobacco Plants", "description": "Drought is a major abiotic stress that affects crop productivity worldwide. Sugarcane can withstand periods of water scarcity during the final stage of culm maturation, during which sucrose accumulation occurs. Meanwhile, prolonged periods of drought can cause severe plant losses.In a previous study, we evaluated the transcriptome of drought-stressed plants to better understand sugarcane responses to drought. Among the up-regulated genes was Scdr1 (sugarcane drought-responsive 1). The aim of the research reported here was to characterize this gene. Scdr1 encodes a putative protein containing 248 amino acids with a large number of proline (19%) and cysteine (13%) residues. Phylogenetic analysis showed that ScDR1is in a clade with homologs from other monocotyledonous plants, separate from those of dicotyledonous plants. The expression of Scdr1 in different varieties of sugarcane plants has not shown a clear association with drought tolerance.The overexpression of Scdr1 in transgenic tobacco plants increased their tolerance to drought, salinity and oxidative stress, as demonstrated by increased photosynthesis, water content, biomass, germination rate, chlorophyll content and reduced accumulation of ROS. Physiological parameters, such as transpiration rate (E), net photosynthesis (A), stomatal conductance (gs) and internal leaf CO(2) concentration, were less affected by abiotic stresses in transgenic Scdr1 plants compared with wild-type plants. Overall, our results indicated that Scdr1 conferred tolerance to multiple abiotic stresses, highlighting the potential of this gene for biotechnological applications.", "keywords": ["Chlorophyll", "Nicotiana", "0301 basic medicine", "Sucrose", "Science", "Molecular Sequence Data", "03 medical and health sciences", "Amino Acid Sequence", "Biomass", "Transgenes", "Photosynthesis", "Phylogeny", "Plant Proteins", "2. Zero hunger", "0303 health sciences", "Base Sequence", "Sequence Homology", " Amino Acid", "Q", "R", "Water", "15. Life on land", "Plants", " Genetically Modified", "6. Clean water", "Droughts", "Saccharum", "Oxidative Stress", "Medicine", "Salts", "Reactive Oxygen Species", "Research Article", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1371%2fjournal.pone.0044697"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371%2fjournal.pone.0044697", "name": "item", "description": "10.1371%2fjournal.pone.0044697", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371%2fjournal.pone.0044697"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-09-11T00:00:00Z"}}, {"id": "11579/193142", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:21:57Z", "type": "Journal Article", "created": "2024-10-10", "title": "Evaluation of per- and polyfluoroalkyl substances (PFAS) toxic effects on the acute inflammatory response in the medicinal leech Hirudo verbana", "description": "Per- and polyfluoroalkyl (PFAS) substances are a large group of chemicals with elevated water and oil-resistance properties, widely implicated in various applicative fields. Due to the extensive use and high resistance to degradative factors, these compounds pose a significant risk of environmental spreading, bioaccumulating also in living organisms. In this context, despite many researches have been performed to demonstrate 'legacy' PFAS harmfulness, only few data are still available about all the emerging fluorinated molecules, industrially introduced to replace the previous ones. For this reason, we proposed the medicinal leech Hirudo verbana as consolidated invertebrate model to assess the effects of four different PFAS (HFPO-DA, PFMoBa, PFOA and PFMOPrA) following freshwater dispersion. Morphological, immunohistochemical and molecular analyses demonstrate that, despite all the compounds basically induce an acute inflammatory and oxidative stress response, a different cellular and molecular response has been observed. Whereas for PFOA and PFMOPrA an increase in the tested concentration leads to a corresponding rise in the immune response, HFPO-DA and PFMoBa trigger an entirely opposite effect. Indeed, the significant recruitment of both granulocytes and macrophage like cells, typically involved in the removal of non-self, is inhibited with increasing concentrations of these compounds. The data collected revealed a different sensitivity of the leech immune system following PFAS exposure, requiring to deepen the current knowledge on the potential toxicity of these compounds.", "keywords": ["Inflammation", "Fluorocarbons", "PFAS", "Freshwater environment", "Inflammatory response", "610", "Toxicology", "Hirudo medicinalis", "Oxidative Stress", "Leeches", "628", "Oxidative stre", "Animals", "Medicinal leech", "Water Pollutants", " Chemical"]}, "links": [{"href": "https://doi.org/11579/193142"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11579/193142", "name": "item", "description": "11579/193142", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11579/193142"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-01T00:00:00Z"}}, {"id": "10447/688224", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:21:47Z", "type": "Journal Article", "created": "2024-08-19", "title": "Photoaging of polystyrene-based microplastics amplifies inflammatory response in macrophages", "description": "The continuous release of municipal and industrial products into the environment poses a growing concern for public health. Among environmental pollutants, polystyrene (PS) stands out as a primary constituent of environmental plastic waste, given its widespread use and high production rates owing to its durability and user-friendly properties. The detection of polystyrene microparticles (PS-MPs) in various living organisms has been well-documented, posing a serious threat due to their potential passage into the human ecosystem. In this manuscript, we aimed to study the toxicological effects of low concentrations of pristine and photoaged PS-MPs in a murine macrophage cell line. To this purpose, PS-MPs were photoaged by indoor exposure to visible light to simulate environmental weathering due to solar irradiation (PS-MPs3h). Physical characterization revealed that the irradiation treatment results in particle degradation and the possible release of nanoparticles. Monocultures of the RAW264.7\u00a0cell line were then exposed to PS-MPs and PS-MPs3h at concentrations comparable to experimental measurements from biological samples, to assess cytotoxicity, intracellular oxidative stress, primary genotoxicity, and inflammatory effects. Significant toxicity-related outcomes were observed in cells treated with both pristine PS-MPs and PS-MPs3h even at low concentrations (0,10\u00a0\u03bcg/ml and 1\u00a0\u03bcg/ml). PS-MPs3h exhibited greater adverse effects compared to PS-MPs, including reduced cell viability, increased ROS production, elevated DNA damage, and upregulation of IL-6 and NOS2 gene expression. Therefore, we can conclude that changes induced by environmental aging in the physicochemical composition of PS microplastics play a crucial role in the adverse health outcomes associated with microplastic exposure.", "keywords": ["Inflammation", "microplastics", " polystyrene", " macrophages", "Cell Survival", "Microplastics", "Macrophages", "Mice", "Oxidative Stress", "RAW 264.7 Cells", "N/A", "Polystyrenes", "Animals", "Environmental Pollutants", "Reactive Oxygen Species", "DNA Damage"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/493702/1/Aloi%20et%20al%20Chemospere%202024.pdf"}, {"href": "https://iris.unipa.it/bitstream/10447/688224/2/Aloi%20et%20al.%202024.pdf"}, {"href": "https://doi.org/10447/688224"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10447/688224", "name": "item", "description": "10447/688224", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10447/688224"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "11353/10.2110681", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:21:54Z", "type": "Journal Article", "created": "2024-11-19", "title": "A respiro-fermentative strategy to survive nanoxia in Acidobacterium capsulatum", "description": "Abstract                <p>Microbial soil habitats are characterized by rapid shifts in substrate and nutrient availabilities, as well as chemical and physical parameters. One such parameter that can vary in soil is oxygen; thus, microbial survival is dependent on adaptation to this substrate. To better understand the metabolic abilities and adaptive strategies to oxygen-deprived environments, we combined genomics with transcriptomics of a model organism, Acidobacterium capsulatum, to explore the effect of decreasing, environmentally relevant oxygen concentrations. The decrease from 10 to 0.1\uffc2\uffa0\uffc2\uffb5M oxygen (3.6 to 0.036 pO2% present atmospheric level, respectively) caused the upregulation of the transcription of genes involved in signal transduction mechanisms, energy production and conversion and secondary metabolites biosynthesis, transport, and catabolism based on clusters of orthologous group categories. Contrary to established observations for aerobic metabolism, key genes in oxidative stress response were significantly upregulated at lower oxygen concentrations, presumably due to an NADH/NAD+ redox ratio imbalance as the cells transitioned into nanoxia. Furthermore, A. capsulatum adapted to nanoxia by inducing a respiro-fermentative metabolism and rerouting fluxes of its central carbon and energy pathways to adapt to high NADH/NAD+ redox ratios. Our results reveal physiological features and metabolic capabilities that allowed A. capsulatum to adapt to oxygen-limited conditions, which could expand into other environmentally relevant soil strains.</p", "keywords": ["0301 basic medicine", "106022 Mikrobiologie", "0303 health sciences", "Acidobacteriota", "NADH imbalances", "microaerobic respiration", "Gene Expression Regulation", " Bacterial", "Adaptation", " Physiological", "oxygen limitation", "Acidobacteria", "Oxygen", "Oxidative Stress", "03 medical and health sciences", "106026 \u00d6kosystemforschung", "Fermentation", "106022 Microbiology", "106026 Ecosystem research", "fermentation", "transcriptome", "Oxidation-Reduction", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/11353/10.2110681"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11353/10.2110681", "name": "item", "description": "11353/10.2110681", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11353/10.2110681"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-11-18T00:00:00Z"}}, {"id": "1887/4172281", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:22:06Z", "type": "Journal Article", "created": "2024-11-12", "title": "Biodegradable microplastics induce profound changes in lettuce (Lactuca sativa) defense mechanisms and to some extent deteriorate growth traits", "description": "The development of agricultural technologies has intensified the use of plastic in this sector. Products of plastic degradation, such as microplastics (MPs), potentially threaten living organisms, biodiversity and agricultural ecosystem functioning. Thus, biodegradable plastic materials have been introduced to agriculture. However, the effects of biodegradable plastic substitutes on soil ecosystems are even less known than those of traditional ones. Here, we studied the effects of environmentally relevant concentrations of MPs prepared from a biodegradable plastic (a starch-polybutylene adipate terephthalate blend, PBAT-BD-MPs) on the growth and defense mechanisms of lettuce (Lactuca sativa) in CLIMECS system (CLImatic Manipulation of ECosystem Samples). PBAT-BD-MPs in the highest concentrations negatively affected some traits of growth, i.e., dry weight percentage, specific leaf area, and both C and N contents. We observed more profound changes in plant physiology and biochemistry, as PBAT-BD-MPs decreased chlorophyll content and triggered a concerted response of plant defense mechanisms against oxidative stress. In conclusion, exposure to PBAT-BD-MPs induced plant oxidative stress and activated plant defense mechanisms, leading to oxidative homeostasis that sustained plant growth and functioning. Our study highlights the need for in-depth understanding of the effect of bioplastics on plants.", "keywords": ["580", "Chlorophyll", "0301 basic medicine", "570", "0303 health sciences", "salicylic acid", "Microplastics", "Lipid peroxidation", "lipid peroxidation", "Salicylic acid", "Biodegradable Plastics", "Plant Leaves", "Oxidative Stress", "03 medical and health sciences", "Starch-polybutylene adipate terephthalate", "Biodegradation", " Environmental", "total phenolic content", "starch-polybutylene adipate terephthalate", "Soil Pollutants", "PBAT", "Total phenolic content", "CLIMECS system", "Lactuca"]}, "links": [{"href": "https://doi.org/1887/4172281"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1887/4172281", "name": "item", "description": "1887/4172281", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1887/4172281"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "20.500.14243/493702", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:22:17Z", "type": "Journal Article", "created": "2024-08-19", "title": "Photoaging of polystyrene-based microplastics amplifies inflammatory response in macrophages", "description": "The continuous release of municipal and industrial products into the environment poses a growing concern for public health. Among environmental pollutants, polystyrene (PS) stands out as a primary constituent of environmental plastic waste, given its widespread use and high production rates owing to its durability and user-friendly properties. The detection of polystyrene microparticles (PS-MPs) in various living organisms has been well-documented, posing a serious threat due to their potential passage into the human ecosystem. In this manuscript, we aimed to study the toxicological effects of low concentrations of pristine and photoaged PS-MPs in a murine macrophage cell line. To this purpose, PS-MPs were photoaged by indoor exposure to visible light to simulate environmental weathering due to solar irradiation (PS-MPs3h). Physical characterization revealed that the irradiation treatment results in particle degradation and the possible release of nanoparticles. Monocultures of the RAW264.7\u00a0cell line were then exposed to PS-MPs and PS-MPs3h at concentrations comparable to experimental measurements from biological samples, to assess cytotoxicity, intracellular oxidative stress, primary genotoxicity, and inflammatory effects. Significant toxicity-related outcomes were observed in cells treated with both pristine PS-MPs and PS-MPs3h even at low concentrations (0,10\u00a0\u03bcg/ml and 1\u00a0\u03bcg/ml). PS-MPs3h exhibited greater adverse effects compared to PS-MPs, including reduced cell viability, increased ROS production, elevated DNA damage, and upregulation of IL-6 and NOS2 gene expression. Therefore, we can conclude that changes induced by environmental aging in the physicochemical composition of PS microplastics play a crucial role in the adverse health outcomes associated with microplastic exposure.", "keywords": ["Inflammation", "Mice", "Oxidative Stress", "RAW 264.7 Cells", "microplastics", " polystyrene", " macrophages", "Cell Survival", "Microplastics", "Macrophages", "Polystyrenes", "Animals", "Environmental Pollutants", "Reactive Oxygen Species", "DNA Damage"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/493702/1/Aloi%20et%20al%20Chemospere%202024.pdf"}, {"href": "https://doi.org/20.500.14243/493702"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.14243/493702", "name": "item", "description": "20.500.14243/493702", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.14243/493702"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "38992384", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:23:02Z", "type": "Journal Article", "created": "2024-07-10", "title": "Reproduction, growth and oxidative stress in earthworm Eisenia andrei exposed to conventional and biodegradable mulching film microplastics", "description": "Plastic contamination in agricultural soils has become increasingly evident. Plastic mulching films are widely used in agricultural practices. However, the increased use of biodegradable plastics has, to some extent, replaced their non-degradable counterparts. The fragmentation of plastics generates microplastics (MPs), posing risk to soil functions and organisms. In this study the effects of low-density polyethylene microplastics (PE-MP) and polybutylene adipate terephthalate biodegradable microplastics (PBAT-BD-MP) originating from mulching films on the earthworm Eisenia andrei were studied. The earthworms were exposed to seven concentrations (0, 0.005, 0.05, 0.1, 0.5, 1, and 5\u00a0% w/w) based on environmentally relevant levels and worst-case scenarios on soil contamination. Survival, growth, reproduction, and biomarkers for oxidative stress [superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST), glutathione (GSH), and lipid peroxidation (LPO)] were analysed. Additionally, the Integrated Biomarker Response Index (IBR) was calculated to assess the overall oxidative stress status of the earthworms. Results showed that PE-MP exposure slightly decreased the biomass of the earthworms towards higher concentrations, whereas PBAT-BD-MPs induced growth at lower concentrations. MPs did not have a significant effect on Eisenia andrei reproduction; however, a slight negative trend was observed in juvenile production with increasing PE-MP concentrations. Both PE-MP and PBAT-BD-MP affected antioxidant system, PE-MPs with changes in CAT and GR levels and PBAT-BD-MPs inducing effects on SOD and LPO levels. Additionally, both MPs exhibited effects on soil parameters, resulting in increased soil pH and water-holding capacity at 5\u00a0% concentration. Changes in soil parameters can further affect soil organisms such as earthworms. This study provides understanding of the ecotoxicological effects of conventional and biodegradable microplastics on the earthworm Eisenia andrei. It also shows that MP particles of both conventional and biodegradable mulching films induce oxidative stress, considered as an early-warning indicator for adverse ecological effects, in environmentally relevant concentrations.", "keywords": ["lierot", "soil ecotoxicology", "LDPE", "Microplastics", "School of Resource Wisdom", "maaper\u00e4biologia", "Biodegradable Plastics", "Resurssiviisausyhteis\u00f6", "maatalous", "Animals", "Soil Pollutants", "PBAT", "Oligochaeta", "oksidatiivinen stressi", "Glutathione Transferase", "mikromuovi", "2. Zero hunger", "maaper\u00e4", "agricultural soil", "Superoxide Dismutase", "Reproduction", "biodegradable plastic", "Catalase", "ymp\u00e4rist\u00f6kuormitus", "biohajoaminen", "environmental stress", "ekotoksikologia", "Oxidative Stress", "maaper\u00e4el\u00e4imist\u00f6", "muovi", "Polyethylene", "13. Climate action", "Lipid Peroxidation", "Biomarkers"]}, "links": [{"href": "https://doi.org/38992384"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "38992384", "name": "item", "description": "38992384", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/38992384"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-01T00:00:00Z"}}, {"id": "39847881", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:23:03Z", "type": "Journal Article", "created": "2024-09-10", "title": "Strategies of Physiological, Morpho-Anatomical and Biochemical Adaptation in Seedlings of Native Species Exposed to Mining Waste", "description": "Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100\u202f% contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10\u202f% to 25\u202f% of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) concentrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2\u00b7- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physicochemical characteristics for plant survival at highest contamination levels.", "keywords": ["Metal", "Soil pollution", "Germination", "Catalase", "Adaptation", " Physiological", "Environmental pollution", "Mining", "Phytoremediation", "Environmental sciences", "Soil", "Prosopis", "Ascorbate Peroxidases", "TD172-193.5", "Oxidative stress", "Seedlings", "Phytotoxicity", "Metals", " Heavy", "Seeds", "Arid environment", "Soil Pollutants", "GE1-350", "Peroxidase"]}, "links": [{"href": "https://doi.org/39847881"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecotoxicology%20and%20Environmental%20Safety", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "39847881", "name": "item", "description": "39847881", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/39847881"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=oxidative+stress&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=oxidative+stress&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=oxidative+stress&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=oxidative+stress&offset=20", "hreflang": "en-US"}], "numberMatched": 20, "numberReturned": 20, "distributedFeatures": [], "timeStamp": "2026-09-20T18:06:32.572025Z"}