Tillage and harvesting operations of perennial forage crops have problems with soil compaction. The effects of this phenomenon are soil deterioration with reduced crop performance and yield. This study aims to assess soil disturbance by measuring the level of compaction caused by the harvesting operations of Phalaris arundinacea L. P. arundinacea is a species that lends itself to biomass production and phytoremediation of contaminated soils; it adapts to difficult soil conditions, outperforming other species in terms of ease of planting, cost, maturity time, yield, and contamination levels. The crop was sown in three plots of the experimental teaching farm of the University of Tuscia, Viterbo, Italy. Following a detailed analysis of the chemical\u2013physical characteristics of the soil, minimum tillage was chosen in order to concentrate on harvesting operations, which were carried out with a disc mower coupled to a tractor. This was followed by penetration resistance and soil moisture measurements to verify the incidence of the operations and the effect of the type of crop on compaction. On the study site, measurements were taken at points that the wheels of the tractor had gone over and at points that they had not. The soil analysis results indicate different chemical\u2013physical characteristics between the two areas, the texture being frankly sandy to clayey. Penetration resistance measurements indicated differences for the first 20 cm between the part that was covered by the tractor\u2019s tyres and the part that was left touched but also between the three plots. Moisture influenced penetration resistance. This study provides an evaluation of the first data obtained from a project that will last four years and which will explore the dynamics between soil, cultivation, and harvesting operations, giving a fundamental basis for further investigation of further harvesting operations and soil characteristics, which are crucial for planning and managing crops and reducing impacts on the soil in order to preserve it.