{"type": "FeatureCollection", "features": [{"id": "10.1007/s13595-014-0444-7", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:15:49Z", "type": "Journal Article", "created": "2014-12-19", "title": "Changes In Soil Phosphorus Fractions After 9 Years Of Continuous Nitrogen Addition In A Larix Gmelinii Plantation", "description": "The key message N addition decreased soil inorganic P availability, microbial biomass P, and acid phosphatase activity in the larch plantation. Soil inorganic P availability decreased after N addition due to the changes in both microbial properties and plant uptake.\u2022 Context Soil phosphorus (P) availability is considered an important factor in influencing the biomass production of plants. Sustained inputs of nitrogen (N) through atmospheric deposition or N fertilizers, particularly in temperate forests, may change the composition and availability of P and thus affect long-term forest productivity.\u2022 Aims The objective of this study was to assess soil P availability, P fractions, and microbial properties including microbial biomass P and acid phosphatase activity after 9 consecutive years of N addition in a larch (Larix gmelinii) plantation, northeastern China.\u2022 Methods From 2003 to 2011, NH4NO3 was added to replicate plots (three 20\u00a0m\u2009\u00d7\u200930\u00a0m plots) in the larch plantation each year at a rate of 100\u00a0kg\u00a0N\u00a0ha\u22121\u00a0year\u22121. Soil samples from 0\u201310-cm and 10\u201320-cm depths were collected in N addition plots and control (no N addition) plots.\u2022 Results N addition significantly decreased soil NaHCO3-Pi (Pi is inorganic P), microbial biomass P, and acid phosphatase activity but increased the NaOH-Pi concentration. N addition appeared to induce a decrease in soil inorganic P availability by changing pH and P uptake by trees. In addition, N addition significantly decreased the NaOH-Po (Po is organic P) concentration, possibly because of increased P mineralization. However, the total P and other P fractions were unaffected by N fertilization.\u2022 Conclusion Our results suggested that N addition enhanced P uptake by trees, whereas it reduced soil inorganic P availability as well as microbial biomass and activity related to soil P cycling in the larch plantation.", "keywords": ["[SDV] Life Sciences [q-bio]", "2. Zero hunger", "Microbial biomass P", "Larch plantation", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "7. Clean energy", "Phosphorus availability", "Phosphatase activity"]}, "links": [{"href": "https://doi.org/10.1007/s13595-014-0444-7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Annals%20of%20Forest%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s13595-014-0444-7", "name": "item", "description": "10.1007/s13595-014-0444-7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s13595-014-0444-7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-12-19T00:00:00Z"}}, {"id": "10.1007/s11104-004-2267-z", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-06-23T16:15:27Z", "type": "Journal Article", "created": "2005-05-31", "title": "Liming And Nitrogen Fertilization Affects Phosphatase Activities, Microbial Biomass And Mycorrhizal Colonisation In Upland Grassland", "description": "We have studied the effects of factorial combinations of lime and N additions on soil microbial biomass, respiration rates and phosphatase activity of an upland grassland. We also used an Agrostis capillaris seedling bioassay to assess the effect of the treatments on the activity of arbuscular-mycorrhizal (AM) fungi and root surface phosphatase enzymes and the concentrations of N and P in the bioassay plant shoots. In the F and H horizons, soil microbial biomass carbon (Cmic) decreased in response to the liming, while addition of lime and N together reduced basal respiration rates. In the Ah horizon, Cmic was unaffected by the treatments but basal respiration rates decreased in the plots receiving nitrogen. Soil phosphatase activity decreased only in the Ah horizon in plots receiving lime, either in combination with N or alone. The mass of root fwt. colonized by AM fungi increased in response to the treatments in the order nitrogenCrops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition strategies of three main crops are affected by the application of sewage sludges, compared with a mineral P fertilizer. We carried out a 3-months greenhouse pot experiment and compared the response of P-acquisition traits among wheat, barley and canola in a soil amended with three sludges or a mineral P fertilizer. Results showed that the P-acquisition strategy differed among crops. Compared with canola, wheat and barley had a higher specific root length and a greater root carboxylate release and they acquired as much P from sludge as from mineral P. By contrast, canola shoot P content was greater with sludge than with mineral P. This was attributed to a higher root-released acid phosphatase activity which promoted the mineralization of sludge-derived P-organic. This study showed that contrasted P-acquisition strategies of crops allows increased use of renewable P resources by optimizing combinations of crop and the type of P fertilizer applied within the cropping system.Saprobic soil fungi drive many important ecosystem processes, including decomposition, and many of their effects are related to growth rate and enzymatic ability. In mycology, there has long been the implicit assumption of a trade-off between growth and enzymatic investment, which we test here using a set of filamentous fungi from the same soil. For these fungi we measured growth rate (as colony radial extension) and enzymatic repertoire (activities of four enzymes: laccase, cellobiohydrolase, leucine aminopeptidase and acid phosphatase), and explored the interaction between the traits based on phylogenetically corrected methods. Our results support the existence of a trade-off, however only for the enzymes presumably representing a larger metabolic cost (laccase and cellobiohydrolase). Our study offers new insights into potential functional complementarity within the soil fungal community in ecosystem processes, and experimentally supports an enzymatic investment/growth rate trade-off underpinning phenomena including substrate succession.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen", " Pilze", " Algen", "Acid Phosphatase", "Laccase", "Fungi", "579", "15. Life on land", "Article", "Fungal Proteins", "Microbial ecology", "Leucyl Aminopeptidase", "03 medical and health sciences", "Cellulose 1", "4-beta-Cellobiosidase", "Fungal ecology", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/360511v1.full.pdf"}, {"href": "https://www.nature.com/articles/s41598-020-68099-8.pdf"}, {"href": "https://doi.org/10.1038/s41598-020-68099-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-020-68099-8", "name": "item", "description": "10.1038/s41598-020-68099-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-020-68099-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-02T00:00:00Z"}}, {"id": "10.1111/gcb.15218", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:26Z", "type": "Journal Article", "created": "2020-06-12", "title": "Long\u2010term nitrogen loading alleviates phosphorus limitation in terrestrial ecosystems", "description": "Abstract

Increased human\uffe2\uff80\uff90derived nitrogen (N) deposition to terrestrial ecosystems has resulted in widespread phosphorus (P) limitation of net primary productivity. However, it remains unclear if and how N\uffe2\uff80\uff90induced P limitation varies over time. Soil extracellular phosphatases catalyze the hydrolysis of P from soil organic matter, an important adaptive mechanism for ecosystems to cope with N\uffe2\uff80\uff90induced P limitation. Here we show, using a meta\uffe2\uff80\uff90analysis of 140 studies and 668 observations worldwide, that N stimulation of soil phosphatase activity diminishes over time. Whereas short\uffe2\uff80\uff90term N loading (\uffe2\uff89\uffa45\uffc2\uffa0years) significantly increased soil phosphatase activity by 28%, long\uffe2\uff80\uff90term N loading had no significant effect. Nitrogen loading did not affect soil available P and total P content in either short\uffe2\uff80\uff90 or long\uffe2\uff80\uff90term studies. Together, these results suggest that N\uffe2\uff80\uff90induced P limitation in ecosystems is alleviated in the long\uffe2\uff80\uff90term through the initial stimulation of soil phosphatase activity, thereby securing P supply to support plant growth. Our results suggest that increases in terrestrial carbon uptake due to ongoing anthropogenic N loading may be greater than previously thought.South China has been experiencing very high rate of acid deposition and severe soil acidification in recent decades, which has been proposed to exacerbate the regional ecosystem phosphorus (P) limitation. We conducted a 10\uffe2\uff80\uff90year field experiment of simulated acid deposition to examine how acidification impacts seasonal changes of different soil P fractions in a tropical forest with highly acidic soils in south China. As expected, acid addition significantly increased occluded P pool but reduced the other more labile P pools in the dry season. In the wet season, however, acid addition did not change microbial P, soluble P and labile organic P pools. Acid addition significantly increased exchangeable Al3+ and Fe3+ and the activation of Fe oxides in both seasons. Different from the decline of microbial abundance in the dry season, acid addition increased ectomycorrhizal fungi and its ratio to arbuscular mycorrhiza fungi in the wet season, which significantly stimulated phosphomonoesterase activities and likely promoted the dissolution of occluded P. Our results suggest that, even in already highly acidic soils, the acidification\uffe2\uff80\uff90induced P limitation could be alleviated by stimulating ectomycorrhizal fungi and phosphomonoesterase activities. The differential responses and microbial controls of seasonal soil P transformation revealed here should be implemented into ecosystem biogeochemical model for predicting plant productivity under future acid deposition scenarios.Acid phosphatases are enzymes that play a crucial role in the hydrolysis of various organophosphorous molecules. A putative acid phosphatase called FS6 was identified using genetic profiles and sequences from different environments. FS6 showed high sequence similarity to type C acid phosphatases and retained more than 30% of consensus residues in its protein sequence. A histidine\uffe2\uff80\uff90tagged recombinant FS6 produced in Escherichia coli exhibited extremophile properties, functioning effectively in a broad pH range between 3.5 and 8.5. The enzyme demonstrated optimal activity at temperatures between 25 and 50\uffc2\uffb0C, with a melting temperature of 51.6\uffc2\uffb0C. Kinetic parameters were determined using various substrates, and the reaction catalysed by FS6 with physiological substrates was at least 100\uffe2\uff80\uff90fold more efficient than with p\uffe2\uff80\uff90nitrophenyl phosphate. Furthermore, FS6 was found to be a decamer in solution, unlike the dimeric forms of crystallized proteins in its family.

The imbalance between nitrogen (N) and phosphorus (P) deposition may shift temperate ecosystems from N\uffe2\uff80\uff90 to P\uffe2\uff80\uff90limitation. However, it is unclear how the imbalanced N\uffc2\uffa0:\uffc2\uffa0P input affects the strategies of plants to acquire P and, therefore, the growth of plants and the competition among species.

We conducted a 4\uffe2\uff80\uff90yr N\uffe2\uff80\uff90addition experiment in young and mature larch (Larix principis\uffe2\uff80\uff90rupprechtii) stands. Plant growth and P acquisition strategies were assessed for larch and understorey vegetation.

N addition stimulated the aboveground productivity of understorey vegetation in the young stand and larch in the mature stand, with other species unaffected. The competitive advantages of understorey vegetation in the young stand and larch in the mature stand were associated with their high stoichiometric homoeostasis. To maintain the N\uffc2\uffa0:\uffc2\uffa0P homoeostasis of these species, an increase in phosphatase activity but not P resorption efficiency increased the supply of P. Additionally, N addition accelerated P mineralization by decreasing the fungal\uffe2\uff80\uff90to\uffe2\uff80\uff90bacterial ratios and improved uptake of soil P by increasing the arbuscular mycorrhizas\uffe2\uff80\uff90to\uffe2\uff80\uff90ectomycorrhizas ratios.

Our results suggest that plants with high stoichiometric homoeostasis could better cope with N deposition\uffe2\uff80\uff90induced P\uffe2\uff80\uff90deficiency. Although P resorption efficiency showed little plasticity in response, plants activated a variety of P\uffe2\uff80\uff90acquisition pathways to alleviate the P\uffe2\uff80\uff90deficiency caused by N deposition.

", "keywords": ["2. Zero hunger", "Analysis of Variance", "Bacteria", "Nitrogen", "Acid Phosphatase", "Fatty Acids", "Fungi", "Larix", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "Phosphates", "Plant Leaves", "Species Specificity", "Mycorrhizae", "Homeostasis", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Phospholipids"]}, "links": [{"href": "https://doi.org/10.1111/nph.14083"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.14083", "name": "item", "description": "10.1111/nph.14083", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.14083"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-07-11T00:00:00Z"}}, {"id": "10.1371/journal.pone.0082661", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:15Z", "type": "Journal Article", "created": "2013-12-20", "title": "Nutrient Limitation In Three Lowland Tropical Forests In Southern China Receiving High Nitrogen Deposition: Insights From Fine Root Responses To Nutrient Additions", "description": "Elevated nitrogen (N) deposition to tropical forests may accelerate ecosystem phosphorus (P) limitation. This study examined responses of fine root biomass, nutrient concentrations, and acid phosphatase activity (APA) of bulk soil to five years of N and P additions in one old-growth and two younger lowland tropical forests in southern China. The old-growth forest had higher N capital than the two younger forests from long-term N accumulation. From February 2007 to July 2012, four experimental treatments were established at the following levels: Control, N-addition (150 kg N ha(-1) yr(-1)), P-addition (150 kg P ha(-1) yr(-1)) and N+P-addition (150 kg N ha(-1) yr(-1) plus 150 kg P ha(-1) yr(-1)). We hypothesized that fine root growth in the N-rich old-growth forest would be limited by P availability, and in the two younger forests would primarily respond to N additions due to large plant N demand. Results showed that five years of N addition significantly decreased live fine root biomass only in the old-growth forest (by 31%), but significantly elevated dead fine root biomass in all the three forests (by 64% to 101%), causing decreased live fine root proportion in the old-growth and the pine forests. P addition significantly increased live fine root biomass in all three forests (by 20% to 76%). The combined N and P treatment significantly increased live fine root biomass in the two younger forests but not in the old-growth forest. These results suggest that fine root growth in all three study forests appeared to be P-limited. This was further confirmed by current status of fine root N:P ratios, APA in bulk soil, and their responses to N and P treatments. Moreover, N addition significantly increased APA only in the old-growth forest, consistent with the conclusion that the old-growth forest was more P-limited than the younger forests.", "keywords": ["2. Zero hunger", "0106 biological sciences", "Analysis of Variance", "China", "Tropical Climate", "Nitrogen", "Science", "Q", "Acid Phosphatase", "R", "Phosphorus", "04 agricultural and veterinary sciences", "Forests", "Hydrogen-Ion Concentration", "15. Life on land", "Plant Roots", "01 natural sciences", "Soil", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Research Article"], "contacts": [{"organization": "Xiankai Lu, Feifei Zhu, Frank S. Gilliam, Jiangming Mo, Muneoki Yoh,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0082661"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0082661", "name": "item", "description": "10.1371/journal.pone.0082661", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0082661"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-12-20T00:00:00Z"}}, {"id": "10.1371/journal.pone.0100443", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:15Z", "type": "Journal Article", "created": "2014-06-23", "title": "Effects Of Winter Cover Crops Residue Returning On Soil Enzyme Activities And Soil Microbial Community In Double-Cropping Rice Fields", "description": "Residue management in cropping systems is useful to improve soil quality. However, the studies on the effects of residue management on the enzyme activities and microbial community of soils in South China are few. Therefore, the effects of incorporating winter cover crop residue with a double-cropping rice (Oryza sativa L.) system on soil enzyme activities and microbial community in Southern China fields were studied. The experiment has conducted at the experimental station of the Institute of Soil and Fertilizer Research, Hunan Academy of Agricultural Science, China since winter 2004. Four winter cropping systems were used: rice-rice-ryegrass (Lolium multiflorum L.) (R-R-Ry), rice-rice-Chinese milk vetch (Astragalus sinicus L.) (R-R-Mv), rice-rice-rape (Brassica napus L.) (R-R-Ra) and rice-rice with winter fallow (R-R-Fa). The result indicated that the enzyme activities in the R-R-Ry, R-R-Mv and R-R-Ra systems were significantly higher (P<0.05) than in the R-R-Fa system during the early and late rice season. The \u03b2-glucosidase activities reached peak values at the tillering stage after residue application, and alkaline phosphatase activities reached peak values at the booting stage after residue application, respectively, the activities of \u03b2-glucosidase and alkaline phosphatase gradually decreased after this. Arylsulfatase activities reached peak values at the maturity stage. Arylamidase activities reached peak values at the maturity stage. The numbers of aerobic bacteria, actinomycete and fungus of residue treatments were significantly higher (P<0.05) than that the R-R-Ra system. However, the number of anaerobic bacteria under the R-R-Ry and R-R-Mv systems was significantly lower (P<0.05) than that under the R-R-Fa system during early rice and late rice growth stage. Thus, incorporation of winter cover crops into rotations may increase enzyme activities and microbial community in soil and therefore improve soil quality.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Science", "beta-Glucosidase", "Q", "R", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "Alkaline Phosphatase", "Arylsulfotransferase", "6. Clean water", "11. Sustainability", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Seasons", "Fertilizers", "Soil Microbiology", "Research Article"], "contacts": [{"organization": "Tang WenGuang, Lin Ye-Chun, Yang GuangLi, Wang Ke, Xiao Xiaoping, Tang Haiming,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0100443"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0100443", "name": "item", "description": "10.1371/journal.pone.0100443", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0100443"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-06-23T00:00:00Z"}}, {"id": "10.1371/journal.pone.0102315", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:16Z", "type": "Journal Article", "created": "2014-07-16", "title": "Soil Biochemical Responses To Nitrogen Addition In A Bamboo Forest", "description": "Many vital ecosystem processes take place in the soils and are greatly affected by the increasing active nitrogen (N) deposition observed globally. Nitrogen deposition generally affects ecosystem processes through the changes in soil biochemical properties such as soil nutrient availability, microbial properties and enzyme activities. In order to evaluate the soil biochemical responses to elevated atmospheric N deposition in bamboo forest ecosystems, a two-year field N addition experiment in a hybrid bamboo (Bambusa pervariabilis \u00d7 Dendrocalamopsis daii) plantation was conducted. Four levels of N treatment were applied: (1) control (CK, without N added), (2) low-nitrogen (LN, 50 kg N ha(-1) year(-1)), (3) medium-nitrogen (MN, 150 kg N ha(-1) year(-1)), and (4) high-nitrogen (HN, 300 kg N ha(-1) year(-1)). Results indicated that N addition significantly increased the concentrations of NH4(+), NO3(-), microbial biomass carbon, microbial biomass N, the rates of nitrification and denitrification; significantly decreased soil pH and the concentration of available phosphorus, and had no effect on the total organic carbon and total N concentration in the 0-20 cm soil depth. Nitrogen addition significantly stimulated activities of hydrolytic enzyme that acquiring N (urease) and phosphorus (acid phosphatase) and depressed the oxidative enzymes (phenol oxidase, peroxidase and catalase) activities. Results suggest that (1) this bamboo forest ecosystem is moving towards being limited by P or co-limited by P under elevated N deposition, (2) the expected progressive increases in N deposition may have a potential important effect on forest litter decomposition due to the interaction of inorganic N and oxidative enzyme activities, in such bamboo forests under high levels of ambient N deposition.", "keywords": ["Analysis of Variance", "China", "Atmosphere", "Monophenol Monooxygenase", "Nitrogen", "Science", "Q", "Acid Phosphatase", "R", "04 agricultural and veterinary sciences", "Forests", "15. Life on land", "Catalase", "Nitrification", "Soil", "Ammonia", "Denitrification", "Medicine", "Urea", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Sasa", "Soil Microbiology", "Research Article", "Peroxidase"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0102315"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0102315", "name": "item", "description": "10.1371/journal.pone.0102315", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0102315"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-07-16T00:00:00Z"}}, {"id": "10.1590/s0100-06832003000300004", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:30Z", "type": "Journal Article", "created": "2005-04-26", "title": "Biomassa Microbiana E Atividade Enzim\u00e1tica Em Solos Sob Vegeta\u00e7\u00e3o Nativa E Sistemas Agr\u00edcolas Anuais E Perenes Na Regi\u00e3o De Primavera Do Leste (Mt)", "description": "

Primavera do Leste \uffc3\uffa9 um dos p\uffc3\uffb3los de produ\uffc3\uffa7\uffc3\uffa3o de gr\uffc3\uffa3os e fibras do Mato Grosso, com lavouras altamente tecnificadas. Este estudo foi realizado num Latossolo Vermelho-Amarelo da regi\uffc3\uffa3o de Primavera, com objetivo de avaliar a biomassa e a atividade microbiana de solos sob vegeta\uffc3\uffa7\uffc3\uffa3o nativa e sistemas agr\uffc3\uffadcolas anuais e perenes. As amostras de solo foram coletadas em duas profundidades (0-5 e 5-20 cm), no in\uffc3\uffadcio da esta\uffc3\uffa7\uffc3\uffa3o chuvosa, em \uffc3\uffa1reas sob cultivo de videira (Vitis vinifera), entrelinha e linha, cultivos anuais (soja) e em uma \uffc3\uffa1rea de vegeta\uffc3\uffa7\uffc3\uffa3o nativa de Cerrad\uffc3\uffa3o. Foram avaliados o carbono da biomassa microbiana (CBM), carbono prontamente mineraliz\uffc3\uffa1vel e as atividades das enzimas beta-glucosidase, fosfatase \uffc3\uffa1cida e arilsulfatase. Nas duas profundidades avaliadas, os sistemas de uso do solo com culturas perenes e anuais apresentaram redu\uffc3\uffa7\uffc3\uffb5es m\uffc3\uffa9dias de 70 % no CBM, em rela\uffc3\uffa7\uffc3\uffa3o \uffc3\uffa0 \uffc3\uffa1rea sob vegeta\uffc3\uffa7\uffc3\uffa3o nativa. O manejo diferenciado na entrelinha do parreiral e a utiliza\uffc3\uffa7\uffc3\uffa3o do capim-p\uffc3\uffa9-de-galinha (Eleusine indica), como cobertura viva, proporcionaram aumentos no C mineraliz\uffc3\uffa1vel e na atividade das enzimas beta-glucosidase e arilsulfatase nas duas profundidades. Os n\uffc3\uffadveis m\uffc3\uffa9dios de P no solo sob Cerrad\uffc3\uffa3o resultaram em valores de atividade da fosfatase \uffc3\uffa1cida inferiores aos dos observados em outros locais do Cerrado. Mesmo assim, na profundidade de 0-5 cm, a atividade da fosfatase \uffc3\uffa1cida no Cerrad\uffc3\uffa3o foi superior \uffc3\uffa0 da entrelinha do parreiral (VE) e \uffc3\uffa0 da \uffc3\uffa1rea com culturas anuais, demonstrando a sua import\uffc3\uffa2ncia na mineraliza\uffc3\uffa7\uffc3\uffa3o do f\uffc3\uffb3sforo org\uffc3\uffa2nico em \uffc3\uffa1reas sob vegeta\uffc3\uffa7\uffc3\uffa3o nativa. Os resultados obtidos confirmaram a sensibilidade dos par\uffc3\uffa2metros microbiol\uffc3\uffb3gicos e bioqu\uffc3\uffadmicos para identificar altera\uffc3\uffa7\uffc3\uffb5es no solo de acordo com os diferentes sistemas de uso da terra.

", "keywords": ["biomass carbon", "arylsulfatase", "2. Zero hunger", "respira\u00e7\u00e3o microbiana", "Cerrado", "carbono da biomassa", "04 agricultural and veterinary sciences", "arilsulfatase", "15. Life on land", "fosfatase \u00e1cida", "vineyards", "beta-glucosidase", "microbial respiration", "acid phosphatase", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "parreiral", "soybean", "soja"]}, "links": [{"href": "https://doi.org/10.1590/s0100-06832003000300004"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20Brasileira%20de%20Ci%C3%AAncia%20do%20Solo", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0100-06832003000300004", "name": "item", "description": "10.1590/s0100-06832003000300004", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0100-06832003000300004"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-06-01T00:00:00Z"}}, {"id": "10.1590/s0100-06832003000300005", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:30Z", "type": "Journal Article", "created": "2005-04-26", "title": "Propriedades Biol\u00f3gicas Em Agregados De Um Latossolo Vermelho-Escuro Sob Plantio Convencional E Direto No Cerrado", "description": "

As distribui\uffc3\uffa7\uffc3\uffb5es do carbono da biomassa microbiana (CBM), da atividade enzim\uffc3\uffa1tica e do C mineraliz\uffc3\uffa1vel foram avaliadas em agregados, coletados na profundidade de 0-0,05 m, de um Latossolo Vermelho-Escuro argiloso, sob vegeta\uffc3\uffa7\uffc3\uffa3o nativa de Cerrado e sob sistemas de plantio direto (PD) e convencional com arado de discos (PC), estabelecidos h\uffc3\uffa1 21 anos. A separa\uffc3\uffa7\uffc3\uffa3o dos agregados foi realizada por via \uffc3\uffbamida. As classes de 8,00-2,00 mm; 0,50-0,25 mm e 0,25-0,106 mm e amostras denominadas soma de agregados foram selecionadas para as determina\uffc3\uffa7\uffc3\uffb5es biol\uffc3\uffb3gicas. Em rela\uffc3\uffa7\uffc3\uffa3o \uffc3\uffa0 \uffc3\uffa1rea nativa, os sistemas cultivados causaram quebra de macroagregados e perda de CBM. A aplica\uffc3\uffa7\uffc3\uffa3o localizada de adubos, o menor revolvimento do solo e os maiores teores de mat\uffc3\uffa9ria org\uffc3\uffa2nica no PD favoreceram, em rela\uffc3\uffa7\uffc3\uffa3o ao PC, a ocorr\uffc3\uffaancia de maiores n\uffc3\uffadveis de fosfatase \uffc3\uffa1cida e arilsulfatase nos agregados e nas amostras que representavam a soma de agregados. Os microagregados e a soma dos agregados do PD tamb\uffc3\uffa9m apresentaram maiores teores de CBM, comparativamente ao PC. As maiores atividades da beta-glucosidase foram observadas nos macro e microagregados do PD. Os sistemas de manejo (PD e PC) influenciaram a distribui\uffc3\uffa7\uffc3\uffa3o das propriedades biol\uffc3\uffb3gicas nos agregados. A atividade das enzimas beta-glucosidase, fosfatase \uffc3\uffa1cida e arilsulfatase foi maior em macroagregados do PD apesar da distribui\uffc3\uffa7\uffc3\uffa3o semelhante do CBM nas tr\uffc3\uffaas classes de agregados avaliadas. No PC, apenas beta-glucosidase apresentou distribui\uffc3\uffa7\uffc3\uffa3o diferenciada entre macro e microagregados.

", "keywords": ["arylsulfatase", "microbial biomass", "Agriculture (General)", "04 agricultural and veterinary sciences", "arilsulfatase", "readily mineralizable C", "fosfatase \u00e1cida", "S1-972", "biomassa microbiana", "beta-glucosidase", "acid phosphatase", "carbono mineraliz\u00e1vel", "enzimas do solo", "0401 agriculture", " forestry", " and fisheries", "soil enzymes"]}, "links": [{"href": "https://doi.org/10.1590/s0100-06832003000300005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20Brasileira%20de%20Ci%C3%AAncia%20do%20Solo", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0100-06832003000300005", "name": "item", "description": "10.1590/s0100-06832003000300005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0100-06832003000300005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-06-01T00:00:00Z"}}, {"id": "10.1590/s1516-35982011000700024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:33Z", "type": "Journal Article", "created": "2017-02-03", "title": "Impact Of Different Grazing Systems For Bovine Cattle On The Soil Microbiological And Chemical Characteristics", "description": "The objective of this work was to study the effect of grazing systems: rotational, continuous and the absence of cattle, in pastures of Brachiaria brizantha on microbial activity (respiratory, potential nitrification, solubilizing, and enzymatic) and on the chemical attributes (contents of organic carbon, total nitrogen, organic phosphorus, and moisture) of Eutrustox soil over one year. Eight Canchim breed animals were used. The samples were collected in the four seasons from 2005 to 2006. The average of organic C, organic P, and moisture contents, as well as potential nitrification and solubilizing activity and urease and acid phosphatase activities were significantly higher in the spring and/or summer than in autumn and/or winter. The total nitrogen amounts did not vary among seasons. While the respiratory activity was larger during winter and summer, cellulase activity was smaller during spring than in the other seasons. Except for organic P, higher values of the variables studied were observed in the rotational grazing. The results indicate that the hot and rainy season and the rotational grazing system cause increases in the soil microbiological and chemical attributes.", "keywords": ["urease", "2. Zero hunger", "cellulase", "550", "potential nitrification", "microbial respiratory activity", "04 agricultural and veterinary sciences", "15. Life on land", "SF1-1100", "630", "phosphatase", "Animal culture", "Cellulase", "0401 agriculture", " forestry", " and fisheries"], "contacts": [{"organization": "Garcia, Martha Regina Lucizano, Sampaio, Alexandre Amstalden Moraes, Nahas, Ely,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1590/s1516-35982011000700024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20Brasileira%20de%20Zootecnia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s1516-35982011000700024", "name": "item", "description": "10.1590/s1516-35982011000700024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s1516-35982011000700024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-07-01T00:00:00Z"}}, {"id": "10.2134/jeq2005.0470", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-06-23T16:20:56Z", "type": "Journal Article", "created": "2006-07-07", "title": "Soil Microbial Communities And Enzyme Activities Under Various Poultry Litter Application Rates", "description": "ABSTRACT

The potential excessive nutrient and/or microbial loading from mismanaged land application of organic fertilizers is forcing changes in animal waste management. Currently, it is not clear to what extent different rates of poultry litter impact soil microbial communities, which control nutrient availability, organic matter quality and quantity, and soil degradation potential. From 2002 to 2004, we investigated the microbial community and several enzyme activities in a Vertisol soil (fine, smectitic, thermic, Udic Haplustert) at 0 to 15 cm as affected by different rates of poultry litter application to pasture (0, 6.7, and 13.4 Mg ha\uffe2\uff88\uff921) and cultivated sites (0, 4.5, 6.7, 9.0, 11.2, and 13.4 Mg ha\uffe2\uff88\uff921) in Texas, USA. No differences in soil pH (average: 7.9), total N (pasture: 2.01\uffe2\uff80\uff933.53, cultivated: 1.09\uffe2\uff80\uff931.98 g kg\uffe2\uff88\uff921 soil) or organic C (pasture average: 25\uffe2\uff80\uff9326.7, cultivated average: 13.9\uffe2\uff80\uff9316.1 g kg\uffe2\uff88\uff921 soil) were observed following the first four years of litter application. Microbial biomass carbon (MBC) and nitrogen (MBN) increased at litter rates greater than 6.7 Mg ha\uffe2\uff88\uff921 (pasture: MBC = >863, MBN = >88 mg kg\uffe2\uff88\uff921 soil) compared to sites with no applied litter (MBC = 722, MBN = 69 mg kg\uffe2\uff88\uff921 soil). Enzyme activities of C (\uffce\uffb2\uffe2\uff80\uff90glucosidase, \uffce\uffb1\uffe2\uff80\uff90galactosidase, \uffce\uffb2\uffe2\uff80\uff90glucosaminidase) or N cycling (\uffce\uffb2\uffe2\uff80\uff90glucosaminidase) were increased at litter rates greater than 6.7 Mg ha\uffe2\uff88\uff921 Enzyme activities of P (alkaline phosphatase) and S (arylsulfatase) mineralization showed the same response in pasture, but they were only increased at the highest (9.0, 11.2, and 13.4 Mg ha\uffe2\uff88\uff921) litter application rates in cultivated sites. According to fatty acid methyl ester (FAME) analysis, the pasture soils experienced shifts to higher bacterial populations at litter rates of 6.7 Mg ha\uffe2\uff88\uff921, and shifts to higher fungal populations at the highest litter application rates in cultivated sites. While rates greater than 6.7 Mg ha\uffe2\uff88\uff921 provided rapid enhancement of the soil microbial populations and enzymatic activities, they result in P application in excess of crop needs. Thus, studies will continue to investigate whether litter application at rates below 6.7 Mg ha\uffe2\uff88\uff921, previously recommended to maintain water quality, will result in similar improved soil microbial and biochemical functioning with continued annual litter application.

", "keywords": ["2. Zero hunger", "Time Factors", "Nitrogen", "Phosphorus", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "15. Life on land", "Alkaline Phosphatase", "Carbon", "Poultry", "6. Clean water", "Manure", "Hexosaminidases", "Animals", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Organic Chemicals", "Soil Microbiology", "Sulfur", "Arylsulfatases"]}, "links": [{"href": "https://doi.org/10.2134/jeq2005.0470"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2005.0470", "name": "item", "description": "10.2134/jeq2005.0470", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2005.0470"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-07-01T00:00:00Z"}}, {"id": "10.3389/fpls.2016.01730", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:21:33Z", "type": "Journal Article", "created": "2016-11-21", "description": "Tillage operation and fertilizer type play important roles in soil properties as far as soil microbial condition is concerned. Information regarding the simultaneous evaluation of the effect of long-term tillage and fertilization on the soil microbial traits of soybean farms is not available. Accordingly, it was hypothesized that, the microbial biomass and enzyme activity, more often than not, respond quickly to changes in soil tillage and fertilization. Therefore, the experiments were aimed at analyzing the responses of soil microbial traits to tillage and fertilization in a soybean field in Kurdistan University, Iran. The field soil is categorized into coarse Loamy, mixed, superactive, calcareous, and mesic Typic Xerorthents. The experiments were arranged in split plot, based on randomized complete block design with three replications. Main plots consisted of long-term (since 2002) tillage systems including conventional tillage (CT), minimum tillage (MT), and no-tillage (NT). Eight fertilization methods were employed in the sub-plots, including (F1): farmyard manure (FYM); (F2): compost; (F3): chemical fertilizers; (F4): FYM + compost; (F5): FYM + chemical fertilizers; (F6): compost + chemical fertilizers; (F7): FYM + compost + chemical fertilizers and (F8): Control (without fertilizer). The highest microbial biomass carbon (385.1 \u03bcg) was observed in NT-F4 treatment. The NT treatment comparatively recorded higher values of acid phosphatase (189.1 \u03bcg PNP g-1 h-1), alkaline phosphatase (2879.6 \u03bcg PNP g-1 h-1) and dehydrogenase activity (68.1 \u03bcg PNP g-1 h-1). The soil treated with a mixture of compost and FYM inputs had the maximum urease activity of all tillage treatments. Organically manured treatment (F4) showed more activity in dehydrogenase (85.7 \u03bcg PNP g-1 h-1), acid phosphatase (199.1 \u03bcg PNP g-1 h-1), and alkaline phosphatase (3183.6 \u03bcg PNP g-1 h-1) compared to those treated with chemical fertilizers. In NT-F4 treatment, using on-farm inputs is most suitable for sustainable management and improvement in soil biological activities in soybean cultivation. We concluded that applying organic manures and employing reduced tillage systems increased soil microbial biomass and enzyme activities.", "keywords": ["urease", "2. Zero hunger", "compost", "microbial biomass", "Plant culture", "Compost", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "Urease", "6. Clean water", "phosphatase", "SB1-1110", "12. Responsible consumption", "dehydrogenase", "tillage", "0401 agriculture", " forestry", " and fisheries", "tillage."]}, "links": [{"href": "https://doi.org/10.3389/fpls.2016.01730"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2016.01730", "name": "item", "description": "10.3389/fpls.2016.01730", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2016.01730"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-11-21T00:00:00Z"}}, {"id": "10045/108728", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:25:33Z", "type": "Journal Article", "created": "2020-06-12", "title": "Long\u2010term nitrogen loading alleviates phosphorus limitation in terrestrial ecosystems", "description": "Abstract

Increased human\uffe2\uff80\uff90derived nitrogen (N) deposition to terrestrial ecosystems has resulted in widespread phosphorus (P) limitation of net primary productivity. However, it remains unclear if and how N\uffe2\uff80\uff90induced P limitation varies over time. Soil extracellular phosphatases catalyze the hydrolysis of P from soil organic matter, an important adaptive mechanism for ecosystems to cope with N\uffe2\uff80\uff90induced P limitation. Here we show, using a meta\uffe2\uff80\uff90analysis of 140 studies and 668 observations worldwide, that N stimulation of soil phosphatase activity diminishes over time. Whereas short\uffe2\uff80\uff90term N loading (\uffe2\uff89\uffa45\uffc2\uffa0years) significantly increased soil phosphatase activity by 28%, long\uffe2\uff80\uff90term N loading had no significant effect. Nitrogen loading did not affect soil available P and total P content in either short\uffe2\uff80\uff90 or long\uffe2\uff80\uff90term studies. Together, these results suggest that N\uffe2\uff80\uff90induced P limitation in ecosystems is alleviated in the long\uffe2\uff80\uff90term through the initial stimulation of soil phosphatase activity, thereby securing P supply to support plant growth. Our results suggest that increases in terrestrial carbon uptake due to ongoing anthropogenic N loading may be greater than previously thought. Acid phosphatases are enzymes that play a crucial role in the hydrolysis of various organophosphorous molecules. A putative acid phosphatase called FS6 was identified using genetic profiles and sequences from different environments. FS6 showed high sequence similarity to type C acid phosphatases and retained more than 30% of consensus residues in its protein sequence. A histidine\uffe2\uff80\uff90tagged recombinant FS6 produced in Escherichia coli exhibited extremophile properties, functioning effectively in a broad pH range between 3.5 and 8.5. The enzyme demonstrated optimal activity at temperatures between 25 and 50\uffc2\uffb0C, with a melting temperature of 51.6\uffc2\uffb0C. Kinetic parameters were determined using various substrates, and the reaction catalysed by FS6 with physiological substrates was at least 100\uffe2\uff80\uff90fold more efficient than with p\uffe2\uff80\uff90 nitrophenyl phosphate. Furthermore, FS6 was found to be a decamer in solution, unlike the dimeric forms of crystallized proteins in its family. Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition strategies of three main crops are affected by the application of sewage sludges, compared with a mineral P fertilizer. We carried out a 3-months greenhouse pot experiment and compared the response of P-acquisition traits among wheat, barley and canola in a soil amended with three sludges or a mineral P fertilizer. Results showed that the P-acquisition strategy differed among crops. Compared with canola, wheat and barley had a higher specific root length and a greater root carboxylate release and they acquired as much P from sludge as from mineral P. By contrast, canola shoot P content was greater with sludge than with mineral P. This was attributed to a higher root-released acid phosphatase activity which promoted the mineralization of sludge-derived P-organic. This study showed that contrasted P-acquisition strategies of crops allows increased use of renewable P resources by optimizing combinations of crop and the type of P fertilizer applied within the cropping system.Increased human\uffe2\uff80\uff90derived nitrogen (N) deposition to terrestrial ecosystems has resulted in widespread phosphorus (P) limitation of net primary productivity. However, it remains unclear if and how N\uffe2\uff80\uff90induced P limitation varies over time. Soil extracellular phosphatases catalyze the hydrolysis of P from soil organic matter, an important adaptive mechanism for ecosystems to cope with N\uffe2\uff80\uff90induced P limitation. Here we show, using a meta\uffe2\uff80\uff90analysis of 140 studies and 668 observations worldwide, that N stimulation of soil phosphatase activity diminishes over time. Whereas short\uffe2\uff80\uff90term N loading (\uffe2\uff89\uffa45\uffc2\uffa0years) significantly increased soil phosphatase activity by 28%, long\uffe2\uff80\uff90term N loading had no significant effect. Nitrogen loading did not affect soil available P and total P content in either short\uffe2\uff80\uff90 or long\uffe2\uff80\uff90term studies. Together, these results suggest that N\uffe2\uff80\uff90induced P limitation in ecosystems is alleviated in the long\uffe2\uff80\uff90term through the initial stimulation of soil phosphatase activity, thereby securing P supply to support plant growth. Our results suggest that increases in terrestrial carbon uptake due to ongoing anthropogenic N loading may be greater than previously thought.South China has been experiencing very high rate of acid deposition and severe soil acidification in recent decades, which has been proposed to exacerbate the regional ecosystem phosphorus (P) limitation. We conducted a 10\uffe2\uff80\uff90year field experiment of simulated acid deposition to examine how acidification impacts seasonal changes of different soil P fractions in a tropical forest with highly acidic soils in south China. As expected, acid addition significantly increased occluded P pool but reduced the other more labile P pools in the dry season. In the wet season, however, acid addition did not change microbial P, soluble P and labile organic P pools. Acid addition significantly increased exchangeable Al3+ and Fe3+ and the activation of Fe oxides in both seasons. Different from the decline of microbial abundance in the dry season, acid addition increased ectomycorrhizal fungi and its ratio to arbuscular mycorrhiza fungi in the wet season, which significantly stimulated phosphomonoesterase activities and likely promoted the dissolution of occluded P. Our results suggest that, even in already highly acidic soils, the acidification\uffe2\uff80\uff90induced P limitation could be alleviated by stimulating ectomycorrhizal fungi and phosphomonoesterase activities. The differential responses and microbial controls of seasonal soil P transformation revealed here should be implemented into ecosystem biogeochemical model for predicting plant productivity under future acid deposition scenarios.Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition strategies of three main crops are affected by the application of sewage sludges, compared with a mineral P fertilizer. We carried out a 3-months greenhouse pot experiment and compared the response of P-acquisition traits among wheat, barley and canola in a soil amended with three sludges or a mineral P fertilizer. Results showed that the P-acquisition strategy differed among crops. Compared with canola, wheat and barley had a higher specific root length and a greater root carboxylate release and they acquired as much P from sludge as from mineral P. By contrast, canola shoot P content was greater with sludge than with mineral P. This was attributed to a higher root-released acid phosphatase activity which promoted the mineralization of sludge-derived P-organic. This study showed that contrasted P-acquisition strategies of crops allows increased use of renewable P resources by optimizing combinations of crop and the type of P fertilizer applied within the cropping system.