{"type": "FeatureCollection", "features": [{"id": "10.1016/j.envint.2020.106190", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:54Z", "type": "Journal Article", "created": "2020-10-26", "title": "Treated wastewater irrigation promotes the spread of antibiotic resistance into subsoil pore-water", "description": "In the present study, we investigated the impact of treated wastewater (TWW) irrigation on the prevalence of antibiotic resistance genes (ARGs) in subsoil pore-water, a so-far under-appreciated matrix. We hypothesized that TWW irrigation increases ARG prevalence in subsoil pore-water. This hypothesis was tested using a multiphase approach, which consisted of sampling percolated subsoil pore-water from lysimeter-wells of a real-scale TWW-irrigated field, operated for commercial farming practices, and controlled, laboratory microcosms irrigated with freshwater or TWW. We monitored the abundance of six selected ARGs (sul1, blaOXA-58, tetM, qnrS, blaCTX-M-32 and blaTEM), the intI1 gene associated with mobile genetic elements and an indicator for anthropogenic pollution and bacterial abundance (16S rRNA gene) by qPCR. The bacterial load of subsoil pore water was independent of both, irrigation intensity in the field study and irrigation water type in the microcosms. Among the tested genes in the field study, sul1 and intI1 exhibited constantly higher relative abundances. Their abundance was further positively correlated with increasing irrigation intensity. Controlled microcosm experiments verified the observed field study results: the relative abundance of several genes, including sul1 and intI1, increased significantly when irrigating with TWW compared to freshwater irrigation. Overall, TWW irrigation promoted the spread of ARGs and intI1 in the subsoil pore-water, while the bacterial load was maintained. The combined results from the real-scale agricultural field and the controlled lab microcosms indicate that the dissemination of ARGs in various subsurface environments needs to be taken into account during TWW irrigation scenarios.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Agricultural Irrigation", "Antibiotic resistance", "Water", "Subsoil pore-water", "Drug Resistance", " Microbial", "Wastewater", "Wastewater reuse", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Environmental sciences", "qPCR", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "RNA", " Ribosomal", " 16S", "ARGs", "GE1-350", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envint.2020.106190"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environment%20International", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envint.2020.106190", "name": "item", "description": "10.1016/j.envint.2020.106190", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envint.2020.106190"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2019.03.176", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:42Z", "type": "Journal Article", "created": "2019-03-13", "title": "Effect of digestate application on microbial respiration and bacterial communities' diversity during bioremediation of weathered petroleum hydrocarbons contaminated soils", "description": "Digestate is an organic by-product of biogas production via anaerobic digestion processes and has a great potential as soil fertilizer due to concentrated nutrients. In this study, we examined digestate as a potential nutrient and microbial seeding for bioremediation of weathered (aged) petroleum hydrocarbon contaminated soils. We analysed 6 different treatments in microcosm using two industrial soils having different textures: a clay rich soil and a sandy soil. After 30\u202fdays of incubation, the highest total petroleum hydrocarbons (TPH) removal was observed in microcosms containing digestate together with bulking agent (17.8% and 12.7% higher than control in clay rich soil and sandy soil, respectively) or digestate together with immobilized bacteria (13.4% and 9% higher than control in clay rich soil and sandy soil, respectively). After digestate application microbial respiration was enhanced in sandy soil and inhibited in clay rich soil due to aggregates formation. After bulking agent addition to clay rich soil aggregates size was reduced and oxygen uptake was improved. Application of digestate to soil resulted in the development of distinct microbial groups in amended and non-amended soils. Genera containing species able to degrade TPH like Acinetobacter and Mycobacterium were abundant in digestate and in soil amended with digestate. Quantification of alkB genes, encoding alkane monoxygenase, revealed high concentration of these genes in digestate bacterial community. After application of digestate, the level of alkB genes significantly increased in soils and remained high until the end of the treatment. The study revealed great potential of digestate as a nutrient and bacteria source for soil bioremediation.", "keywords": ["[SDE] Environmental Sciences", "TPH removal", "550", "Soil remediation", "0211 other engineering and technologies", "alkB genes; Organic fertilizers; qPCR; Soil remediation; TPH removal; Biodegradation", " Environmental; Environmental Restoration and Remediation; Hydrocarbons; Petroleum; Petroleum Pollution; Soil; Soil Pollutants; Soil Microbiology", "02 engineering and technology", "01 natural sciences", "7. Clean energy", "630", "Organic fertilizers", "Environmental", "Soil", "alkB genes", "Soil Pollutants", "Petroleum Pollution", "Environmental Restoration and Remediation", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "[SDE.IE]Environmental Sciences/Environmental Engineering", "15. Life on land", "Hydrocarbons", "6. Clean water", "qPCR", "Biodegradation", " Environmental", "Petroleum", "[SDE]Environmental Sciences", "Biodegradation", "[SDE.IE] Environmental Sciences/Environmental Engineering"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2019.03.176"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2019.03.176", "name": "item", "description": "10.1016/j.scitotenv.2019.03.176", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2019.03.176"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1016/j.biocontrol.2025.105723", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:33Z", "type": "Journal Article", "created": "2024-10-01", "title": "Advancing Strain-Specific Taqman Assays for Trichoderma Asperellum Detection in Commercial Agricultural Settings", "description": "The global agricultural sector is facing significant challenges in achieving higher sustainability, which has increased interest in using biological control agents (BCAs) to manage plant diseases. However, it is essential to ensure that microbial-based products, such as BCAs, are utilised in a manner that does not harm soil quality and fertility while decreasing reliance on synthetic pesticides. To accomplish this, it is crucial to monitor the fate and persistence of bioinoculants in the soil, which is essential for optimising their application over time, as well as for regulatory and commercial purposes and environmental risk assessment. A qPCR detection method utilising TaqMan chemistry is proposed, which has demonstrated high specificity and sensitivity in detecting Trichoderma asperellum, a common BCA species, in soil. The primers and probe were designed based on the \u03b2tubulin2 gene. The TaqMan-based assay was applied and validated on soils where tomatoes and strawberries were grown after a previous application of T. asperellum FC80 strain over three years. The TaqMan-based assay was able to detect the target strain accurately, meeting the stringent requirements for commercial and regulatory applications. Significance and impact of the study: The TaqMan assay developed here has the potential to impact the agricultural sector significantly. It can be used for regulatory, commercial, and scientific purposes to track, monitor, and determine the presence and fate of T. asperellum under field crop conditions, thereby contributing to adopting more sustainable and efficient agricultural practices.", "keywords": ["traceability qPCR", "Soil", "TaqMan Assay", "S", "QH301-705.5", "Fungi Trichoderma asperellum", "Agriculture", "Biology (General)", "Biological control agent"]}, "links": [{"href": "https://doi.org/10.1016/j.biocontrol.2025.105723"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biological%20Control", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biocontrol.2025.105723", "name": "item", "description": "10.1016/j.biocontrol.2025.105723", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biocontrol.2025.105723"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.1016/j.envres.2019.108608", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:57Z", "type": "Journal Article", "created": "2019-07-26", "title": "Antibiotic resistance gene distribution in agricultural fields and crops. A soil-to-food analysis", "description": "Despite the social concern about the generalization of antibiotic resistance hotspots worldwide, very little is known about the contribution of different potential sources to the global risk. Here we present a quantitative analysis of the distribution of Antibiotic Resistance Genes (ARGs) in soil, rhizospheric soil, roots, leaves and beans in tomato, lettuce and broad beans crops (165 samples in total), grown in nine commercial plots distributed in four geographical zones in the vicinity of Barcelona (North East Spain). We also analyzed five soil samples from a nearby forest, with no record of agricultural activities. DNA samples were analyzed for their content in the ARGs sul1, tetM, qnrS1, blaCTX-M-32, blaOXA-58, mecA, and blaTEM, plus the integron intI1, using qPCR methods. In addition, soil microbiomes from the different plots were analyzed by amplicon-targeted 16S rRNA gene sequencing. Our data show a decreasing gradient of ARG loads from soil to fruits and beans, the latter showing only from 0.1 to 0.01% of the abundance values in soil. The type of crop was the main determinant for both ARG distribution and microbiome composition among the different plots, with minor contributions of geographic location and irrigation water source. We propose that soil amendment and/or fertilization, more than irrigation water, are the main drivers of ARG loads on the edible parts of the crop, and that they should therefore be specifically controlled.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Microbiomes", "Agriculture", "Drug Resistance", " Microbial", "Irrigation water", "15. Life on land", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "qPCR", "Soil", "03 medical and health sciences", "Antibiotic resistance genes", "Genes", " Bacterial", "Spain", "RNA", " Ribosomal", " 16S", "Rhizosphere", "Endophytes", "Food Analysis", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envres.2019.108608"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envres.2019.108608", "name": "item", "description": "10.1016/j.envres.2019.108608", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envres.2019.108608"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2018.10.268", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:42Z", "type": "Journal Article", "created": "2018-10-22", "title": "Antibiotic resistance genes distribution in microbiomes from the soil-plant-fruit continuum in commercial Lycopersicon esculentum fields under different agricultural practices", "description": "While the presence of antibiotic resistance genes (ARGs) in agricultural soils and products has been firmly established, their distribution among the different plant parts and the contribution of agricultural practices, including irrigation with reclaimed water, have not been adequately addressed yet. To this end, we analyzed the levels of seven ARGs (sul1, blaTEM, blaCTX-M-32, mecA, qnrS1, tetM, blaOXA-58), plus the integrase gene intl1, in soils, roots, leaves, and fruits from two commercial tomato fields irrigated with either unpolluted groundwater or from a channel impacted by treated wastewater, using culture-independent, quantitative real-time PCR methods. ARGs and intl1 sequences were found in leaves and fruits at levels representing from 1 to 10% of those found in roots or soil. The relative abundance of intl1 sequences correlated with tetM, blaTEM, and sul1 levels, suggesting a high horizontal mobility potential for these ARGs. High-throughput 16S rDNA sequencing revealed microbiome differences both between sample types (soil plus roots versus leaves plus fruits) and sampling zones, and a correlation between the prevalence of Pseudomonadaceae and the levels of different ARGs, particularly in fruits and leaves. We concluded that both microbiome composition and ARGs levels in plants parts, including fruits, were likely influenced by agricultural practices.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Antibiotic resistance", "Microbiota", "Microbiomes", "Agriculture", "Drug Resistance", " Microbial", "Horizontal gene transfer", "Irrigation water", "15. Life on land", "6. Clean water", "qPCR", "Soil", "03 medical and health sciences", "Solanum lycopersicum", "Genes", " Bacterial", "Fruit", "Soil Microbiology", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2018.10.268"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2018.10.268", "name": "item", "description": "10.1016/j.scitotenv.2018.10.268", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2018.10.268"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-01T00:00:00Z"}}, {"id": "10.1080/01490451.2014.908981", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:07Z", "type": "Journal Article", "created": "2015-08-19", "title": "Response Of Soil Denitrifying Communities To Long-Term Prescribed Burning In Two Australian Sclerophyll Forests", "description": "Low-intensity prescribed burning is a common forest management tool and plays a major role in modifying biogeochemical cycling through the alteration of substrate availability and microbial communities. In this study, we assessed the response of microbial community to repeated prescribed burning in two sclerophyll forests (the Bauple site, dry, annual rainfall 1000\u00a0mm; and the Peachester site, wet, 1711\u00a0mm) in southeast Queensland, Australia. At the dry sclerophyll forest (the Bauple site), annual and triennial burning did not significantly alter the soil carbon (C) and nitrogen (N) content, while at the wet scleophyll forest (the Peachester site), two yearly burnings resulted in significantly lower soil total C and N contents compared to the long unburnt treatment. In spite of these different responses, prescribed burning regimes did not significantly influence the abundance of 16S rRNA or denitrifying gene (<i>nar</i>G, <i>nir</i>K, <i>nir</i>S, <i>nos</i>Z) at both sites. These results indicated that, long-term prescribed burning has little effect on the denitrifying communities, while it has varying effects on soil chemical properties at the two sites, which are likely to be explained by differences in vegetation type and soil moisture regime.", "keywords": ["580", "550", "FoR 0403 (Geology)", "denitrifying community", "Geology", "04 agricultural and veterinary sciences", "15. Life on land", "long-term repeated burning", "Microbiology", "3. Good health", "FoR 0605 (Microbiology)", "qPCR", "Soil biology", "13. Climate action", "sclerophyll forest", "0401 agriculture", " forestry", " and fisheries", "soil moisture"]}, "links": [{"href": "https://doi.org/10.1080/01490451.2014.908981"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Geomicrobiology%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1080/01490451.2014.908981", "name": "item", "description": "10.1080/01490451.2014.908981", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1080/01490451.2014.908981"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-08-09T00:00:00Z"}}, {"id": "10.1093/jambio/lxac048", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:18Z", "type": "Journal Article", "created": "2023-03-17", "title": "Two species-specific TaqMan-based quantitative polymerase chain reaction assays for the detection in soil ofPaenibacillus polymyxainocula", "description": "AbstractAims<p>The increasingly widespread use of beneficial microbial inocula in agriculture gives rise to two primary needs: i) the assessment of the environmental risk, i.e. their impact on local soil microbiome and soil properties; ii) being able to track them and monitor their persistence and fate to both optimize their formulation and application method. In previous years, PCR-based methods have detected bacterial or fungal bioinoculant at the species or strain level. However, the selective detection, quantification, and monitoring of target microbial species in a complex ecosystem such as soil require that the tests possess high specificity and sensitivity.</p>Methods and results<p>The work proposes a quantitative real-time PCR detection method using TaqMan chemistry, showing high specificity and sensitivity for the Paenibacillus polymyxa K16 strain. The primer and probe sets were designed using the polymyxin gene cluster targeting pmxC and pmxE sequences. Validation tests showed that these assays allowed a discriminant and specific detection of P. polymyxa K16 in soil.</p>Conclusion<p>The TaqMan-assay developed could thus ensure the necessary level of discrimination required by commercial and regulatory purposes to detect and monitor the bioinoculant in soil.</p", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "TaqMan probes", "molecular markers", "Bioinoculant", "bioinoculant", "polymyxin gene", "Real-Time Polymerase Chain Reaction", "Sensitivity and Specificity", "6. Clean water", "03 medical and health sciences", "qPCR", "Soil", "TaqMan Probe", "PGPR", "RNA", "Paenibacillus polymyxa", "Paenibacillus", "Ecosystem", "DNA Primers"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/453423/1/FPinzari_Two%20species-specific%20TaqMan-based%20quantitative%20assays_453423_2023.pdf"}, {"href": "https://doi.org/10.1093/jambio/lxac048"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Applied%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jambio/lxac048", "name": "item", "description": "10.1093/jambio/lxac048", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jambio/lxac048"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-15T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2007.00394.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:56Z", "type": "Journal Article", "created": "2007-10-19", "title": "Quantitation And Diversity Analysis Of Ruminal Methanogenic Populations In Response To The Antimethanogenic Compound Bromochloromethane", "description": "Methyl coenzyme-M reductase A (mcrA) clone libraries were generated from microbial DNA extracted from the rumen of cattle fed a roughage diet with and without supplementation of the antimethanogenic compound bromochloromethane. Bromochloromethane reduced total methane emissions by c. 30%, with a resultant increase in propionate and branched chain fatty acids. The mcrA clone libraries revealed that Methanobrevibacter spp. were the dominant species identified. A decrease in the incidence of Methanobrevibacter spp. from the clone library generated from bromochloromethane treatment was observed. In addition, a more diverse methanogenic population with representatives from Methanococcales, Methanomicrobiales and Methanosacinales orders was observed for the bromochloromethane library. Sequence data generated from these libraries aided in the design of an mcrA-targeted quantitative PCR (qPCR) assay. The reduction in methane production by bromochloromethane was associated with an average decrease of 34% in the number of methanogenic Archaea when monitored with this qPCR assay. Dissociation curve analysis of mcrA amplicons showed a clear difference in melting temperatures for Methanobrevibacter spp. (80-82 degrees C) and all other methanongens (84-86 degrees C). A decrease in the intensity of the Methanobrevibacter spp. specific peak and an increase for the other peak in the bromochloromethane-treated animals corresponded with the changes within the clone libraries.", "keywords": ["Male", "0301 basic medicine", "Rumen", "Bromochloromethane", "Methanogens", "Molecular Sequence Data", "Euryarchaeota", "Methanobrevibacter", "Polymerase Chain Reaction", "630", "03 medical and health sciences", "2402 Applied Microbiology and Biotechnology", "Animals", "Methyl coenzyme-M reductase", "Phylogeny", "Gene Library", "2. Zero hunger", "0303 health sciences", "Hydrocarbons", " Halogenated", "2404 Microbiology", "Sequence Analysis", " DNA", "mcrA", "qPCR", "DNA", " Archaeal", "Cattle", "Oxidoreductases", "2303 Ecology", "Methane"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2007.00394.x", "name": "item", "description": "10.1111/j.1574-6941.2007.00394.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-12-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2018.01176", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:20:41Z", "type": "Journal Article", "created": "2018-06-08", "title": "Enhanced Bacterial Fitness Under Residual Fluoroquinolone Concentrations Is Associated With Increased Gene Expression in Wastewater-Derived qnr Plasmid-Harboring Strains", "description": "Plasmids harboring qnr genes confer resistance to low fluoroquinolone concentrations. These genes are of significant clinical, evolutionary and environmental importance, since they are widely distributed in a diverse array of natural and clinical environments. We previously extracted and sequenced a large (\u223c185 Kbp) qnrB-harboring plasmid, and several small (\u223c8 Kbp) qnrS-harboring plasmids, from Klebsiella pneumoniae isolates from municipal wastewater biosolids, and hypothesized that these plasmids provide host bacteria a selective advantage in wastewater treatment plants (WWTPs) that often contain residual concentrations of fluoroquinolones. The objectives of this study were therefore to determine the effect of residual fluoroquinolone concentrations on the growth kinetics of qnr plasmid-harboring bacteria; and on the copy number of qnr plasmids and expression of qnr genes. Electrotransformants harboring either one of the two types of plasmids could grow at ciprofloxacin concentrations exceeding 0.5 \u03bcg ml-1, but growth was significantly decreased at concentrations higher than 0.1 \u03bcg ml-1. In contrast, plasmid-free strains failed to grow even at 0.05 \u03bcg ml-1. No differences were observed in plasmid copy number under the tested ciprofloxacin concentrations, but qnr expression increased incrementally from 0 to 0.4 \u03bcg ml-1, suggesting that the transcription of this gene is regulated by antibiotic concentration. This study reveals that wastewater-derived qnr plasmids confer a selective advantage in the presence of residual fluoroquinolone concentrations and provides a mechanistic explanation for this phenomenon.", "keywords": ["0301 basic medicine", "0303 health sciences", "qnr genes", "Microbiology", "QR1-502", "6. Clean water", "fitness", "3. Good health", "wastewater treatment", "03 medical and health sciences", "qPCR expression analysis", "plasmid", "11. Sustainability", "qnr genes", " wastewater treatment", " plasmid", " qPCR expression analysis", " fitness"], "contacts": [{"organization": "Roberto B. M. Marano, Roberto B. M. Marano, Edouard Jurkevitch, Eddie Cytryn, Ella Kaplan, Ella Kaplan,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2018.01176"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2018.01176", "name": "item", "description": "10.3389/fmicb.2018.01176", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2018.01176"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-06-08T00:00:00Z"}}, {"id": "10.3390/agronomy12020450", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:20:48Z", "type": "Journal Article", "created": "2022-02-14", "title": "The Analysis of Partial Sequences of the Flavonone 3 Hydroxylase Gene in Lupinus mutabilis Reveals Differential Expression of Two Paralogues Potentially Related to Seed Coat Colour", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Flavonone 3 hydroxylases (EC 1.14.11.9) are key enzymes in the synthesis of anthocyanins and other flavonoids. Such compounds are involved in seed coat colour and stem pigmentation. Lupinus mutabilis (tarwi) is a legume crop domesticated in the Andean region, valued for the high protein and oil content of its seeds. Tarwi accessions are being selected for cultivation in Europe under defined breeding criteria. Seed coat colour patterns are relevant breeding traits in tarwi, and these are conditioned by anthocyanin content. We identified and isolated part of the tarwi flavonone 3-hydroxylase gene (LmF3h) from two accessions with distinct seed coat colour patterns. Two partial LmF3h paralogues, with predicted 20% amino-acid changes but little predicted tertiary structure alterations, were identified in the coloured seed genotype, while only one was present in the white seed genotype. Upon selection and validation of appropriate reference genes, a RT-qPCR analysis showed that these paralogues have different levels of expression during seed development in both genotypes, although they follow the same expression patterns. DNA and transcription analyses enabled to highlight potential F3H paralogues relatable to seed coat pigmentation in tarwi and, upon biochemical and genetic confirmation, prompt marker-assisted breeding for relevant phenotypic traits associated with flavonoid synthesis.</p></article>", "keywords": ["0301 basic medicine", "2. Zero hunger", "<i>F3H</i>", "0303 health sciences", "S", "Agriculture", "<i>Lupinus mutabilis</i>", "03 medical and health sciences", "F3H", "seed coat", "gene expression", "Lupinus mutabilis", "<i>Lupinus mutabilis</i>; <i>F3H</i>; seed coat; gene expression; RT-qPCR reference genes", "RT-qPCR reference genes"]}, "links": [{"href": "http://www.mdpi.com/2073-4395/12/2/450/pdf"}, {"href": "https://doi.org/10.3390/agronomy12020450"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/agronomy12020450", "name": "item", "description": "10.3390/agronomy12020450", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/agronomy12020450"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-11T00:00:00Z"}}, {"id": "10.34894/MIRO5P", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:21:09Z", "type": "Dataset", "title": "Replication Data for: Priming of soil organic matter: chemical structure of added compounds is more important than the energy content", "description": "In March 2014 soil (0-10cm) was collected in Dennenkamp (the Netherlands), a former arable site that has developed into a natural grassland. In the laboratory, fresh soil was sieved (4 mm), homogenized and stored at 4 \u00baC until further use. We report the following data: - CO2 evolution after addition of 13C labelled substrates (glucose, cellobiose and vanillic acid) to the soil. - Abundance of microbial groups (fungi and/or bacteria) that used labeled and non-labeled carbon, measured as increase in 16S rRNA gene copy numbers and 18SrDNA copy numbers, for bacteria and fungi respectively, per g of soil. - Biomass yield (for fungi and bacteria separately) for three substrates (glucose, cellobiose and vanillic acid), used that as a proxy for microbial usable energy", "keywords": ["2. Zero hunger", "Earth and Environmental Science", "qPCR data", "Earth and Environmental Sciences", "Verwerkte data", "Processed data", "15. Life on land", "Environmental Research", "Natural Sciences", "CO2 data", "Geosciences"], "contacts": [{"organization": "Di Lonardo, D.P.", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.34894/MIRO5P"}, {"rel": "self", "type": "application/geo+json", "title": "10.34894/MIRO5P", "name": "item", "description": "10.34894/MIRO5P", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.34894/MIRO5P"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-01T00:00:00Z"}}, {"id": "10400.5/23455", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:24:37Z", "type": "Journal Article", "created": "2022-02-13", "title": "The Analysis of Partial Sequences of the Flavonone 3 Hydroxylase Gene in Lupinus mutabilis Reveals Differential Expression of Two Paralogues Potentially Related to Seed Coat Colour", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Flavonone 3 hydroxylases (EC 1.14.11.9) are key enzymes in the synthesis of anthocyanins and other flavonoids. Such compounds are involved in seed coat colour and stem pigmentation. Lupinus mutabilis (tarwi) is a legume crop domesticated in the Andean region, valued for the high protein and oil content of its seeds. Tarwi accessions are being selected for cultivation in Europe under defined breeding criteria. Seed coat colour patterns are relevant breeding traits in tarwi, and these are conditioned by anthocyanin content. We identified and isolated part of the tarwi flavonone 3-hydroxylase gene (LmF3h) from two accessions with distinct seed coat colour patterns. Two partial LmF3h paralogues, with predicted 20% amino-acid changes but little predicted tertiary structure alterations, were identified in the coloured seed genotype, while only one was present in the white seed genotype. Upon selection and validation of appropriate reference genes, a RT-qPCR analysis showed that these paralogues have different levels of expression during seed development in both genotypes, although they follow the same expression patterns. DNA and transcription analyses enabled to highlight potential F3H paralogues relatable to seed coat pigmentation in tarwi and, upon biochemical and genetic confirmation, prompt marker-assisted breeding for relevant phenotypic traits associated with flavonoid synthesis.</p></article>", "keywords": ["0301 basic medicine", "2. Zero hunger", "<i>F3H</i>", "0303 health sciences", "S", "Agriculture", "<i>Lupinus mutabilis</i>", "03 medical and health sciences", "F3H", "seed coat", "gene expression", "Lupinus mutabilis", "<i>Lupinus mutabilis</i>; <i>F3H</i>; seed coat; gene expression; RT-qPCR reference genes", "RT-qPCR reference genes"]}, "links": [{"href": "http://www.mdpi.com/2073-4395/12/2/450/pdf"}, {"href": "https://repositorio.ulisboa.pt/bitstream/10400.5/23455/1/PTALHINHAS-agronomy-12-00450.pdf"}, {"href": "https://doi.org/10400.5/23455"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10400.5/23455", "name": "item", "description": "10400.5/23455", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10400.5/23455"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-11T00:00:00Z"}}, {"id": "20.500.14243/453423", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:25:18Z", "type": "Journal Article", "created": "2023-03-17", "title": "Two species-specific TaqMan-based quantitative polymerase chain reaction assays for the detection in soil ofPaenibacillus polymyxainocula", "description": "AbstractAims<p>The increasingly widespread use of beneficial microbial inocula in agriculture gives rise to two primary needs: i) the assessment of the environmental risk, i.e. their impact on local soil microbiome and soil properties; ii) being able to track them and monitor their persistence and fate to both optimize their formulation and application method. In previous years, PCR-based methods have detected bacterial or fungal bioinoculant at the species or strain level. However, the selective detection, quantification, and monitoring of target microbial species in a complex ecosystem such as soil require that the tests possess high specificity and sensitivity.</p>Methods and results<p>The work proposes a quantitative real-time PCR detection method using TaqMan chemistry, showing high specificity and sensitivity for the Paenibacillus polymyxa K16 strain. The primer and probe sets were designed using the polymyxin gene cluster targeting pmxC and pmxE sequences. Validation tests showed that these assays allowed a discriminant and specific detection of P. polymyxa K16 in soil.</p>Conclusion<p>The TaqMan-assay developed could thus ensure the necessary level of discrimination required by commercial and regulatory purposes to detect and monitor the bioinoculant in soil.</p", "keywords": ["2. Zero hunger", "TaqMan probes", "molecular markers", "bioinoculant", "polymyxin gene", "Real-Time Polymerase Chain Reaction", "Sensitivity and Specificity", "6. Clean water", "qPCR", "Soil", "PGPR", "RNA", "Paenibacillus polymyxa", "Paenibacillus", "Ecosystem", "DNA Primers"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/453423/1/FPinzari_Two%20species-specific%20TaqMan-based%20quantitative%20assays_453423_2023.pdf"}, {"href": "https://academic.oup.com/jambio/article-pdf/134/1/lxac048/49094737/lxac048.pdf"}, {"href": "https://doi.org/20.500.14243/453423"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Applied%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.14243/453423", "name": "item", "description": "20.500.14243/453423", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.14243/453423"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-15T00:00:00Z"}}, {"id": "26f8e218-ae63-43a5-b566-b968bd7a2137", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[9.68, 53.7], [9.68, 53.7], [9.68, 53.7], [9.68, 53.7], [9.68, 53.7]]]}, "properties": {"themes": [{"concepts": [{"id": "farming"}], "scheme": "https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_TopicCategoryCode"}, {"concepts": [{"id": "Soil"}, {"id": "qPCR"}, {"id": "Streptomyces"}], "scheme": "AGROVOC Multilingual agricultural thesaurus"}, {"concepts": [{"id": "opendata"}, {"id": "apple replant disease"}, {"id": "M.26"}, {"id": "EMR.2"}, {"id": "G.935"}, {"id": "Illumina-MiSeq"}], "scheme": "Individual"}, {"concepts": [{"id": "Boden"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}, {"concepts": [{"id": "Europe"}, {"id": "Germany"}, {"id": "Schleswig-Holstein"}], "scheme": "individual"}], "rights": "Restrictions applied to assure the protection of privacy or intellectual property, and any special restrictions or limitations or warnings on using the resource or metadata. Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of the BonaRes Module A-Project - BonaRes - ORDIAmur's research activities.\" Although every care has been taken in preparing and testing the data, the BonaRes Module A-Project - BonaRes - ORDIAmur and the BonaRes Data Centre cannot guarantee that the data are correct; neither does the BonaRes Module A-Project - BonaRes - ORDIAmur and the BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The BonaRes Module A-Project - BonaRes - ORDIAmur and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data.", "updated": "2026-01-15", "type": "Dataset", "created": "2025-12-02", "language": "eng", "title": "ORDIAmur Central Experiment 10 - Rhizosphere Bacteria Reads", "description": "Illumina-MiSeq sequencing was performed using the primer pair 515F/806R to amplify a bacterial fragment of the 16S rRNA gene (V4 region). The sequences were bioinformatically processed, annotated and reads were rarefied to 14,401 reads. The raw sequences are available at NCBI.\n\nGeneral description see mother table: (https://doi.org/10.20387/bonares-r5cg-rx85); Related datasets are listed in the metadata element 'Related Identifier'.\nDataset version 1.0", "formats": [{"name": "CSV"}], "keywords": ["Soil", "qPCR", "Streptomyces", "opendata", "apple replant disease", "M.26", "EMR.2", "G.935", "Illumina-MiSeq", "Boden", "Europe", "Germany", "Schleswig-Holstein"], "contacts": [{"name": "Leibniz Centre for Agricultural Landscape Research", "organization": "ZALF", "position": "Computation and Data Service Platform - Workgroup Research Data Management", "roles": ["publisher"], "phones": [{"value": "+49 33432 82 300"}], "emails": [{"value": "dataservice@zalf.de"}], "addresses": [{"deliveryPoint": ["Eberswalder Strasse 84"], "city": "M\u00fcncheberg", "administrativeArea": "Brandenburg", "postalCode": "15374", "country": "Germany"}], "links": [{"href": null}]}, {"name": "Nils Orth", "organization": "Leibniz University Hannover", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "orth@baum.uni-hannover.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0009-0006-1026-9881", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Kristin Hauschild", "organization": "Julius K\u00fchn Institute", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "kristin.hauschild@ufz.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0001-5261-9583", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Silvia Gschwendtner", "organization": "Helmholtz Munich", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "silvia.gschwendtner@helmholtz-munich.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0002-6379-3728", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Fatma M. Mahmoud", "organization": "Helmholtz Munich", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "fatma.mahmoud@helmholtz-muenchen.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0002-2997-4242", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Sarah Benning", "organization": "Helmholtz Munich", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "sarahmabenning@gmail.com"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0009-0002-2025-9645", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Traud Winkelmann", "organization": "Leibniz University Hannover", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "winkelmann@zier.uni-hannover.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0002-2509-1418", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Traud Winkelmann", "organization": "Leibniz University Hannover", "position": null, "roles": ["projectLeader"], "phones": [{"value": null}], "emails": [{"value": "winkelmann@zier.uni-hannover.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0002-2509-1418", "name_url": "", "description": "0000-0002-2509-1418", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"organization": "Julius K\u00fchn Institute;Leibniz University Hannover;Helmholtz Munich", "roles": ["contributor"]}], "title_alternate": "Data collection: Part 2/4, table: Rhizosphere Bacteria Reads"}, "links": [{"href": "https://maps.bonares.de/mapapps/resources/apps/bonares/index.html?lang=en&mid=26f8e218-ae63-43a5-b566-b968bd7a2137", "rel": "information"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/8bcd5787-19b2-4fa2-8af7-45a7c8c5533f", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "26f8e218-ae63-43a5-b566-b968bd7a2137", "name": "item", "description": "26f8e218-ae63-43a5-b566-b968bd7a2137", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/26f8e218-ae63-43a5-b566-b968bd7a2137"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2026-01-15T00:00:00Z"}}, {"id": "d5a4ef96-cd8e-4fed-bfa6-457e85f1ff19", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[9.68, 53.7], [9.68, 53.7], [9.68, 53.7], [9.68, 53.7], [9.68, 53.7]]]}, "properties": {"license": "CC BY", "rights": "Restrictions applied to assure the protection of privacy or intellectual property, and any special restrictions or limitations or warnings on using the resource or metadata. Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of the BonaRes Module A-Project - BonaRes - ORDIAmur's research activities.\" Although every care has been taken in preparing and testing the data, the BonaRes Module A-Project - BonaRes - ORDIAmur and the BonaRes Data Centre cannot guarantee that the data are correct; neither does the BonaRes Module A-Project - BonaRes - ORDIAmur and the BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The BonaRes Module A-Project - BonaRes - ORDIAmur and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data.", "updated": "2026-01-09", "type": "Service", "created": "2025-12-02", "language": "eng", "title": "Web Map Service of the dataset 'ORDIAmur Central Experiment 10 '", "description": "This Web Map Service includes spatial information used by datasets 'ORDIAmur Central Experiment 10'", "keywords": ["infoMapAccessService", "Soil", "qPCR", "Streptomyces"], "contacts": [{"name": "Leibniz Centre for Agricultural Landscape Research", "organization": "ZALF", "position": "Computation and Data Service Platform - Workgroup Research Data Management", "roles": ["publisher"], "phones": [{"value": "+49 33432 82 300"}], "emails": [{"value": "dataservice@zalf.de"}], "addresses": [{"deliveryPoint": ["Eberswalder Strasse 84"], "city": "M\u00fcncheberg", "administrativeArea": "Brandenburg", "postalCode": "15374", "country": "Germany"}], "links": [{"href": null}]}, {"name": "Nils Orth", "organization": "Leibniz University Hannover", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "orth@baum.uni-hannover.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0009-0006-1026-9881", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Kristin Hauschild", "organization": "Julius K\u00fchn Institute", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "kristin.hauschild@ufz.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0001-5261-9583", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Silvia Gschwendtner", "organization": "Helmholtz Munich", "position": null, "roles": ["author"], "phones": [{"value": null}], "emails": [{"value": "silvia.gschwendtner@helmholtz-munich.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": null}], "links": [{"href": {"url": null, "protocol": null, "protocol_url": "", "name": "0000-0002-6379-3728", "name_url": "", "description": "ORCID", "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}, {"name": "Fatma M. 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Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of the BonaRes Module A-Project - BonaRes - ORDIAmur's research activities.\" Although every care has been taken in preparing and testing the data, the BonaRes Module A-Project - BonaRes - ORDIAmur and the BonaRes Data Centre cannot guarantee that the data are correct; neither does the BonaRes Module A-Project - BonaRes - ORDIAmur and the BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The BonaRes Module A-Project - BonaRes - ORDIAmur and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data.", "updated": "2026-01-15", "type": "Dataset", "created": "2025-12-02", "language": "eng", "title": "ORDIAmur Central Experiment 10", "description": "Young apple rootstocks (M.26, EMR.2 and G.935) were grown on apple replant disease affected soil or gamma-irradiated ARD soil (disinfected control) for eight weeks in a greenhouse. Roots were collected, surface disinfected and the genomic DNA was extracted.  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