{"type": "FeatureCollection", "features": [{"id": "10.1007/s00442-012-2578-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:55:31Z", "type": "Journal Article", "created": "2013-01-07", "title": "Effects Of Drought And N-Fertilization On N Cycling In Two Grassland Soils", "description": "Open AccessOecologia, 171 (3)", "keywords": ["[SDE] Environmental Sciences", "N2O fluxes", "550", "functional genes", "Nitrogen", "[SDV]Life Sciences [q-bio]", "Climate", "Climate Change", "Nitrification and denitrification", "enzyme activites", "Urine", "630", "10127 Institute of Evolutionary Biology and Environmental Studies", "Soil", "Quantitative PCR", "Climate change; Enzyme activities; Functional genes; Quantitative PCR; Nitrification and denitrification; N2O fluxes", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Animals", "Climate change", "Enzyme activities", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "Ecosystem", "Soil Microbiology", "Functional genes", "Nitrogen Cycle", "Plants", "Archaea", "Droughts", "[SDV] Life Sciences [q-bio]", "1105 Ecology", " Evolution", " Behavior and Systematics", "climate change", "Genes", " Bacterial", "[SDE]Environmental Sciences", "quantitative PCR", "Denitrification", "570 Life sciences; biology", "590 Animals (Zoology)", "Cattle", "nitrification and denitrification"]}, "links": [{"href": "https://doi.org/10.1007/s00442-012-2578-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-012-2578-3", "name": "item", "description": "10.1007/s00442-012-2578-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-012-2578-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-01-08T00:00:00Z"}}, {"id": "10.1007/s11104-013-1855-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:55:58Z", "type": "Journal Article", "created": "2013-08-21", "title": "Soil Microorganisms Respond To Five Years Of Climate Change Manipulations And Elevated Atmospheric Co2 In A Temperate Heath Ecosystem", "description": "Soil microbial responses to global change can affect organic matter turnover and nutrient cycling thereby altering the overall ecosystem functioning. In a large-scale experiment, we investigated the impact of 5\u00a0years of climate change and elevated atmospheric CO2 on soil microorganisms and nutrient availability in a temperate heathland. The future climate was simulated by increased soil temperature (+0.3\u00a0\u00b0C), extended pre-summer drought (excluding 5\u20138\u00a0% of the annual precipitation) and elevated CO2 (+130\u00a0ppm) in a factorial design. Soil organic matter and nutrient pools were analysed and linked to microbial measures by quantitative PCR of bacteria and fungi, chloroform fumigation extraction, and substrate-induced respiration to assess their impact of climate change on nutrient availability. Warming resulted in higher measures of fungi and bacteria, of microbial biomass and of microbial growth potential, however, this did not reduce the availability of nitrogen or phosphorus in the soil. Elevated CO2 did not directly affect the microbial measures or nutrient pools, whereas drought shifted the microbial community towards a higher fungal dominance. Although we were not able to show strong interactive effects of the global change factors, warming and drought changed both nutrient availability and microbial community composition in the heathland soil, which could alter the ecosystem carbon and nutrient flow in the long-term.", "keywords": ["Bacterial abundance", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Soil nutrient pools", "Elevated carbon dioxide", "CLIMAITE", "Fungal abundance", "15. Life on land", "Real-time quantitative PCR", "6. Clean water", "Long-term ecosystem manipulation", "03 medical and health sciences", "13. Climate action", "11. Sustainability", "Deschampsia flexuosa"]}, "links": [{"href": "https://doi.org/10.1007/s11104-013-1855-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-013-1855-1", "name": "item", "description": "10.1007/s11104-013-1855-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-013-1855-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-08-22T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2010.04.006", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:56:31Z", "type": "Journal Article", "created": "2010-05-22", "title": "Effect Of The Endogeic Earthworm Pontoscolex Corethrurus On The Microbial Structure And Activity Related To Co2 And N2o Fluxes From A Tropical Soil (Madagascar)", "description": "Abstract   The objective of this laboratory study was to determine the influence of a tropical endogeic earthworm,  Pontoscolex corethrurus , on CO 2  and N 2 O fluxes from a tropical Ferralsol and microorganisms potentially involved in these gases emissions. CO 2  and N 2 O fluxes were measured during 35 days from soil mesocosms with and without earthworms. At the end of the incubation, 7% of soil was egested as cast in the earthworm treatment. Then, casts which may be aged from few hours to 35 days old were isolated from non-ingested soil. Different descriptive parameters (activity, density, and structure) of the microbial communities were investigated in the control, the non-ingested soils, and the casts. Quantitative PCR of denitrification genes encoding the nitrite ( nirK ) and nitrous oxide ( nosZ ) reductases was used to study denitrifier density in the earthworm casts. The presence of  P. corethrurus  induced a significant increase in CO 2  emissions but did not affect N 2 O fluxes when measured at mesocosm level. Despite the absence of significant differences in C and N contents between soils and casts, the near infra-red spectra analysis clearly underlined a specific organic signature for the casts. Fungal and bacterial biomass significantly decreased (\u223c2-fold) in casts compared to parent soil, but the fungal-to-bacterial ratio was not modified by the earthworm casting activity. Data suggested that bacterial communities, especially denitrifiers, were modified in casts. The relative abundance of  nirK  and  nosZ  genes increased in the casts while the genetic structures of total bacteria and denitrifying communities were slightly modified in the casts. This study highlighted the importance of earthworm casts as a specific soil habitat where a subset of soil functional bacterial communities (such as denitrifiers) found favourable condition for their growth. However the effect of  P. corethrurus  was less evident when up-scaling from casts to mesocosm level.", "keywords": ["Pontoscolex corethrurus", "Quantitative PCR", "Denitrification genes", "Madagascar", "0401 agriculture", " forestry", " and fisheries", "Microbial activity potentials", "04 agricultural and veterinary sciences", "01 natural sciences", "630", "FAME", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2010.04.006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2010.04.006", "name": "item", "description": "10.1016/j.apsoil.2010.04.006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2010.04.006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-07-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2018.09.128", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:56:56Z", "type": "Journal Article", "created": "2018-09-28", "title": "A rationale for the high limits of quantification of antibiotic resistance genes in soil", "description": "The determination of values of abundance of antibiotic resistance genes (ARGs) per mass of soil is extremely useful to assess the potential impacts of relevant sources of antibiotic resistance, such as irrigation with treated wastewater or manure application. Culture-independent methods and, in particular, quantitative PCR (qPCR), have been regarded as suitable approaches for such a purpose. However, it is arguable if these methods are sensitive enough to measure ARGs abundance at levels that may represent a risk for environmental and human health. This study aimed at demonstrating the range of values of ARGs quantification that can be expected based on currently used procedures of DNA extraction and qPCR analyses. The demonstration was based on the use of soil samples spiked with known amounts of wastewater antibiotic resistant bacteria (ARB) (Enterococcus faecalis, Escherichia coli, Acinetobacter johnsonii, or Pseudomonas aeruginosa), harbouring known ARGs, and also on the calculation of expected values determined based on qPCR. The limits of quantification (LOQ) of the ARGs (vanA, qnrS, blaTEM, blaOXA, blaIMP, blaVIM) were observed to be approximately 4 log-units per gram of soil dry weight, irrespective of the type of soil tested. These values were close to the theoretical LOQ values calculated based on currently used DNA extraction methods and qPCR procedures. The observed LOQ values can be considered extremely high to perform an accurate assessment of the impacts of ARGs discharges in soils. A key message is that ARGs accumulation will be noticeable only at very high doses. The assessment of the impacts of ARGs discharges in soils, of associated risks of propagation and potential transmission to humans, must take into consideration this type of evidence, and avoid the simplistic assumption that no detection corresponds to risk absence.", "keywords": ["0301 basic medicine", "2. Zero hunger", "LOD - Limit of detection", "0303 health sciences", "Acinetobacter", "Drug Resistance", " Microbial", "Wastewater", "Real-Time Polymerase Chain Reaction", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Manure", "Quantitative PCR", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "13. Climate action", "Pseudomonas aeruginosa", "Enterococcus faecalis", "Escherichia coli", "LOQ - Limit of quantification", "Soil Microbiology", "Risk assessment"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2018.09.128"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2018.09.128", "name": "item", "description": "10.1016/j.envpol.2018.09.128", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2018.09.128"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-01T00:00:00Z"}}, {"id": "10.1016/j.jece.2018.02.022", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:57:21Z", "type": "Journal Article", "created": "2018-02-14", "title": "Inter-laboratory calibration of quantitative analyses of antibiotic resistance genes", "description": "Backgrounds: Antibiotic resistant bacteria and antibiotic resistance genes (ARGs) are major human-health threats, widely distributed in the environment. Quantitative PCR (qPCR) is a standard approach to detect and quantify ARGs in environmental compartments. However, the comparison of gene quantification reported by different laboratories is challenging since data are predominantly obtained under non- harmonized conditions, using different qPCR protocols. Objectives: The aim of this study was to develop and calibrate standardized qPCR procedures for quantification of key ARGs, analyzing the same samples with common protocols and distinct equipment, reagents batches and operators. Methods: Treated wastewater from three European countries were processed immediately after collection and transported to the laboratory for total DNA extraction. DNA extracts from each sample were pooled and aliquots were distributed by five partners involved in the calibration procedure. The genes 16S rRNA, vanA, blaTEM, qnrS, sul1, blaCTXM-32 and intI1 were analyzed using harmonized qPCR protocols and the constructed pNORM1 plasmid, which contains fragments of the seven targeted genes, was used for generating standard curves. Conclusions: The 16S rRNA gene was the most abundant, followed by sul1, intI1, qnrS and blaTEM. Quantifications made by different partners were reproducible and inter-laboratory variation was &lt; 20%. The notorious exception was for the qnrS gene, and therefore protocol improvement is recommended. The genes blaCTXM-32 and vanA were below the limit of quantification in most or all of the samples analyzed. The inter-laboratory calibration is an adequate approach to reliably assess ARG abundance and environmental contamination in different environments and geographic locations.", "keywords": ["Life sciences; biology", "info:eu-repo/classification/ddc/570", "0301 basic medicine", "570", "biology", "Inter-laboratory calibration", "Antibiotic resistance gene", "Wastewater", "Life sciences", "01 natural sciences", "6. Clean water", "3. Good health", "Quantitative PCR", "03 medical and health sciences", "ddc:570", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jece.2018.02.022"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Chemical%20Engineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jece.2018.02.022", "name": "item", "description": "10.1016/j.jece.2018.02.022", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jece.2018.02.022"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-02-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2018.05.016", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-31T06:57:50Z", "type": "Journal Article", "created": "2018-05-19", "title": "Experimentally testing the species-habitat size relationship on soil bacteria: A proof of concept", "description": "Abstract   The species-area relationship is one of the most widely reported ecological theories accounting for biodiversity of plants and animals. However, we lack solid experimental data demonstrating whether this key ecological theorem also applies in the microbial world. Here, we conducted a microcosm study to evaluate the role of habitat area in driving the diversity, abundance, composition and functioning (i.e., four enzyme activities linked to organic matter decomposition) of soil bacterial communities. Thus, we aim to evaluate whether the principle of species-area relationship is potentially applicable to soil microbes. We established a fully factorial experimental design of three island sizes (\u223c9, 50 and 150\u202fcm2) by two sterile soils (low, high resources). After six months of glasshouse incubation, habitat-area was positively related to bacterial richness, relative abundance of Chloroflexi, Verrucomicrobia and \u03b4-proteobacteria, and soil functions in both soils. Soil with higher resources always had the greatest bacterial richness and functions. Our findings provide a proof of concept by demonstrating the potential importance of both habitat-area and resource availability in driving soil bacterial biodiversity and functioning.", "keywords": ["2. Zero hunger", "habitat (ecology)", "XXXXXX - Unknown", "Bacterial diversity; Decomposition; Ecological theory; Miseq Illumina; Extracellular enzyme activities; quantitative PCR.", "soil biodiversity", "15. Life on land", "biodegradation", "soil microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2018.05.016"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2018.05.016", "name": "item", "description": "10.1016/j.soilbio.2018.05.016", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2018.05.016"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-08-01T00:00:00Z"}}, {"id": "10.3390/genes13050850", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:01:41Z", "type": "Journal Article", "created": "2022-05-11", "title": "Short-Term Responses of Soil Microbial Communities to Changes in Air Temperature, Soil Moisture and UV Radiation", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>We analyzed the effects on a soil microbial community of short-term alterations in air temperature, soil moisture and ultraviolet radiation and assessed the role of invertebrates (species Enchytraeus crypticus) in modulating the community\u2019s response to these factors. The reference soil, Lufa 2.2, was incubated for 48 h, with and without invertebrates, under the following conditions: standard (20 \u00b0C + 50% water holding capacity (WHC)); increased air temperature (15\u201325 \u00b0C or 20\u201330 \u00b0C + 50% WHC); flood (20 \u00b0C + 75% WHC); drought (20 \u00b0C + 25% WHC); and ultraviolet radiation (UV) (20 \u00b0C + 50% WHC + UV). BIOLOG EcoPlates and 16S rDNA sequencing (Illumina) were used to assess the microbial community\u2019s physiological profile and the bacterial community\u2019s structure, respectively. The bacterial abundance (estimated by 16S rDNA qPCR) did not change. Most of the conditions led to an increase in microbial activity and a decrease in diversity. The structure of the bacterial community was particularly affected by higher air temperatures (20\u201330 \u00b0C, without E. crypticus) and floods (with E. crypticus). Effects were observed at the class, genera and OTU levels. The presence of invertebrates mostly resulted in the attenuation of the observed effects, highlighting the importance of considering microbiome\u2013invertebrate interactions. Considering future climate changes, the effects described here raise concern. This study provides fundamental knowledge to develop effective strategies to mitigate these negative outcomes. However, long-term studies integrating biotic and abiotic factors are needed.</p></article>", "keywords": ["0301 basic medicine", "Soil invertebrates", "Ultraviolet Rays", "drought", "microbial activity", "DNA", " Ribosomal", "Flood", "Article", "Quantitative PCR", "Soil", "03 medical and health sciences", "soil microbiome", "2. Zero hunger", "metagenomics", "increased temperature; drought; flood; UV exposure; microbial activity; bacterial diversity; metagenomics; quantitative PCR; soil microbiome; soil invertebrates", "Soil microbiome", "0303 health sciences", "Drought", "Bacteria", "Microbiota", "bacterial diversity", "Temperature", "Water", "flood", "15. Life on land", "soil invertebrates", "6. Clean water", "UV exposure", "Microbial activity", "Bacterial diversity", "13. Climate action", "quantitative PCR", "Metagenomics", "Increased temperature", "increased temperature"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/13/5/850/pdf"}, {"href": "https://doi.org/10.3390/genes13050850"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes13050850", "name": "item", "description": "10.3390/genes13050850", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes13050850"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-05-10T00:00:00Z"}}, {"id": "10.5061/dryad.79cnp5htw", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:02:13Z", "type": "Dataset", "title": "Data from: A tipping-point in carbon storage when forest expands into tundra is related to mycorrhizal recycling of nitrogen", "description": "unspecifiedTundra ecosystems are global belowground sinks for atmospheric CO2.  Ongoing warming-induced encroachment by shrubs and trees risks turning  this sink into a CO2 source, resulting in a positive feedback on climate  warming. To advance mechanistic understanding of how shifts in mycorrhizal  types affect long-term carbon (C) and nitrogen (N) stocks, we studied  small-scale soil depth profiles of fungal communities and C-N dynamics  across a subarctic-alpine forest-heath vegetation gradient. Belowground  organic stocks decreased abruptly at the transition from heath to forest,  linked to the presence of certain tree-associateds ectomycorrhizal fungi  that contribute to decomposition when mining N from organic matter. In  contrast, ericoid mycorrhizal plants and fungi were associated with  organic matter accumulation and slow decomposition. If climatic controls  on arctic-alpine forest lines are relaxed, increased decomposition will  likely outbalance increased plant productivity, decreasing the overall C  sink capacity of displaced tundra.", "keywords": ["C-N dynamics", "ectomycorrhizal exploration type", "functional genes", "ergosterol", "ITS2 meta-barcoding", "Fungal community", "Arctic greening", "Climate feedback", "15. Life on land", "litter saprotrophs", "mycorrhizal type", "litter bags", "13. Climate action", "soil solution", "FOS: Biological sciences", "soil carbon storage", "quantitative PCR", "soil profiles", "Ectomycorrhizal fungal community", "Ericoid Mycorrhiza", "treeline ecotone"], "contacts": [{"organization": "Clemmensen, Karina E, Durling, Mikael B, Michelsen, Anders, Hallin, Sara, Finlay, Roger D, Lindahl, Bj\u00f6rn D,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.79cnp5htw"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.79cnp5htw", "name": "item", "description": "10.5061/dryad.79cnp5htw", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.79cnp5htw"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-28T00:00:00Z"}}, {"id": "10400.14/37827", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-31T07:05:18Z", "type": "Journal Article", "created": "2022-05-10", "title": "Short-Term Responses of Soil Microbial Communities to Changes in Air Temperature, Soil Moisture and UV Radiation", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>We analyzed the effects on a soil microbial community of short-term alterations in air temperature, soil moisture and ultraviolet radiation and assessed the role of invertebrates (species Enchytraeus crypticus) in modulating the community\u2019s response to these factors. The reference soil, Lufa 2.2, was incubated for 48 h, with and without invertebrates, under the following conditions: standard (20 \u00b0C + 50% water holding capacity (WHC)); increased air temperature (15\u201325 \u00b0C or 20\u201330 \u00b0C + 50% WHC); flood (20 \u00b0C + 75% WHC); drought (20 \u00b0C + 25% WHC); and ultraviolet radiation (UV) (20 \u00b0C + 50% WHC + UV). BIOLOG EcoPlates and 16S rDNA sequencing (Illumina) were used to assess the microbial community\u2019s physiological profile and the bacterial community\u2019s structure, respectively. The bacterial abundance (estimated by 16S rDNA qPCR) did not change. Most of the conditions led to an increase in microbial activity and a decrease in diversity. The structure of the bacterial community was particularly affected by higher air temperatures (20\u201330 \u00b0C, without E. crypticus) and floods (with E. crypticus). Effects were observed at the class, genera and OTU levels. The presence of invertebrates mostly resulted in the attenuation of the observed effects, highlighting the importance of considering microbiome\u2013invertebrate interactions. Considering future climate changes, the effects described here raise concern. This study provides fundamental knowledge to develop effective strategies to mitigate these negative outcomes. However, long-term studies integrating biotic and abiotic factors are needed.</p></article>", "keywords": ["0301 basic medicine", "Soil invertebrates", "Ultraviolet Rays", "drought", "microbial activity", "DNA", " Ribosomal", "Flood", "Article", "Quantitative PCR", "Soil", "03 medical and health sciences", "soil microbiome", "2. Zero hunger", "metagenomics", "increased temperature; drought; flood; UV exposure; microbial activity; bacterial diversity; metagenomics; quantitative PCR; soil microbiome; soil invertebrates", "Soil microbiome", "0303 health sciences", "Drought", "Bacteria", "Microbiota", "bacterial diversity", "Temperature", "Water", "flood", "15. Life on land", "soil invertebrates", "6. Clean water", "UV exposure", "Microbial activity", "Bacterial diversity", "13. Climate action", "quantitative PCR", "Metagenomics", "Increased temperature", "increased temperature"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/13/5/850/pdf"}, {"href": "https://doi.org/10400.14/37827"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10400.14/37827", "name": "item", "description": "10400.14/37827", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10400.14/37827"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-05-10T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=quantitative+PCR&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=quantitative+PCR&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=quantitative+PCR&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=quantitative+PCR&offset=9", "hreflang": "en-US"}], "numberMatched": 9, "numberReturned": 9, "distributedFeatures": [], "timeStamp": "2026-05-31T13:56:07.388333Z"}