{"type": "FeatureCollection", "features": [{"id": "10.1002/ece3.8676", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:14:28Z", "type": "Journal Article", "created": "2022-03-08", "title": "Effects of operational taxonomic unit inference methods on soil microeukaryote community analysis using long-read metabarcoding", "description": "Abstract<p>Long amplicon metabarcoding has opened the door for phylogenetic analysis of the largely unknown communities of microeukaryotes in soil. Here, we amplified and sequenced the ITS and LSU regions of the rDNA operon (around 1500\uffc2\uffa0bp) from grassland soils using PacBio SMRT sequencing. We tested how three different methods for generation of operational taxonomic units (OTUs) effected estimated richness and identified taxa, and how well large\uffe2\uff80\uff90scale ecological patterns associated with shifting environmental conditions were recovered in data from the three methods. The field site at Kungs\uffc3\uffa4ngen Nature Reserve has drawn frequent visitors since Linnaeus's time, and its species rich vegetation includes the largest population of Fritillaria meleagris in Sweden. To test the effect of different OTU generation methods, we sampled soils across an abrupt moisture transition that divides the meadow community into a Carex acuta dominated plant community with low species richness in the wetter part, which is visually distinct from the mesic\uffe2\uff80\uff90dry part that has a species rich grass\uffe2\uff80\uff90dominated plant community including a high frequency of F.\uffc2\uffa0meleagris. We used the moisture and plant community transition as a framework to investigate how detected belowground microeukaryotic community composition was influenced by OTU generation methods. Soil communities in both moisture regimes were dominated by protists, a large fraction of which were taxonomically assigned to Ciliophora (Alveolata) while 30%\uffe2\uff80\uff9340% of all reads were assigned to kingdom Fungi. Ecological patterns were consistently recovered irrespective of OTU generation method used. However, different methods strongly affect richness estimates and the taxonomic and phylogenetic resolution of the characterized community with implications for how well members of the microeukaryotic communities can be recognized in the data.</p>", "keywords": ["580", "species hypothesis", "Ekologi", "0301 basic medicine", "0303 health sciences", "Ecology", "rDNA", "Biological Systematics", "15. Life on land", "03 medical and health sciences", "14. Life underwater", "ITS", "Research Articles", "clustering"]}, "links": [{"href": "https://pub.epsilon.slu.se/27699/1/eshghi-sahraei-s-et-al-220505.pdf"}, {"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.8676"}, {"href": "https://doi.org/10.1002/ece3.8676"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.8676", "name": "item", "description": "10.1002/ece3.8676", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.8676"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-01T00:00:00Z"}}, {"id": "10.1016/j.still.2005.08.001", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:17:40Z", "type": "Journal Article", "created": "2005-09-09", "title": "Soil Aggregation And Bacterial Community Structure As Affected By Tillage And Cover Cropping In The Brazilian Cerrados", "description": "<p>Microbial-based indicators of soil quality are believed to be more dynamic than those based on physical and chemical properties. Recent developments in molecular biology based techniques have led to rapid and reliable tools to characterize microbial community structures. We determined the effects of conventional and no-tillage in cropping systems with and without cover crops on bacterial community structure, total organic carbon (TOC) and soil aggregation. Tillage and rotation did not affect TOC from bulk soil. However, TOC was greater in the largest aggregate size class (7.98-19 mm), and had greater mean-weight diameter under no-tillage than under conventional tillage in the 0-5 cm soil layer. Soil bacterial community structure, based on denaturing gradient gel electrophoresis of polymerase chain reaction amplified DNA (PCR/DGGE) using two different genes as biomarkers, 16S rRNA and rpoB genes, indicated different populations in response to cultivation, tillage and depth, but not due to cover cropping. Soil bacterial community structure and meanweight diameter of soil aggregates indicated alterations in soil conditions due to tillage system. (c) 2005 Elsevier B.V. All rights reserved.</p>", "keywords": ["2. Zero hunger", "GENES", "Cerrados", "16S RIBOSOMAL-RNA", "no-tillage", "04 agricultural and veterinary sciences", "15. Life on land", "DGGE profiling", "SUSTAINABILITY", "PCR", "16S rDNA", "GRADIENT GEL-ELECTROPHORESIS", "MANAGEMENT", "0401 agriculture", " forestry", " and fisheries", "NO-TILLAGE", "HETEROGENEITY", "DGGE", "soil structure", "rpoB", "MICROBIAL DIVERSITY"]}, "links": [{"href": "https://doi.org/10.1016/j.still.2005.08.001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20and%20Tillage%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.still.2005.08.001", "name": "item", "description": "10.1016/j.still.2005.08.001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.still.2005.08.001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-11-01T00:00:00Z"}}, {"id": "10.1080/15324982.2016.1177749", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:18:55Z", "type": "Journal Article", "created": "2016-06-17", "title": "Arbuscular Mycorrhizal Fungal Community Of Wheat Under Long-Term Mineral And Organic Amendments In Semi-Arid Mediterranean Turkey", "description": "A minimal amount of information is currently available concerning arbuscular mycorrhizal (AM) fungal associations with crops in semi-arid zones on Leptosols in Turkey. Therefore, using molecular ecological techniques, we studied the effects of different management practices (without fertilization, chemical fertilization, farmyard manure, and plant compost amendments) on AM fungal communities associated with wheat roots. Experiments were conducted in a field established in 1996 in southern Mediterranean Turkey where soil productivity is low owing to unfavorable climatic effects and soil characteristics. We determined 201 partial sequences of AM fungal nuclear ribosomal large subunit genes. The higher AM fungal richness was found in the control treatment without fertilization and plant compost treatments compared with the chemical fertilization and farmyard manure treatments. Clones related to <i>Rhizophagus</i> were found in all treatments and accounted for 37% of the total AM fungal clones, whereas those of <i>Funneliformis</i> were dominant under chemical fertilization. Redundancy analysis based on the frequency of operational taxonomic units revealed that AM fungal communities were divided into three groups, namely, the control treatment, the chemical fertilization treatment, and the organic treatments (farmyard manure and plant compost treatments). Although different organic amendments supported relatively similar AM fungal communities, plant compost induced higher AM fungal richness than farmyard manure fertilization.", "keywords": ["2. Zero hunger", "Leptosol", "southern Mediterranean Turkey", "organic fertilization", "large ribosomal subunits (LSU rDNA)", "Arbuscular mycorrhizal fungi", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "community analysis"]}, "links": [{"href": "https://doi.org/10.1080/15324982.2016.1177749"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Arid%20Land%20Research%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1080/15324982.2016.1177749", "name": "item", "description": "10.1080/15324982.2016.1177749", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1080/15324982.2016.1177749"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-06-17T00:00:00Z"}}, {"id": "10.3897/zse.100.128096", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:10Z", "type": "Journal Article", "created": "2024-07-25", "title": "Oleaxonchium olearum gen. et sp. nov. (Nematoda, Dorylaimida) associated with an olive grove in the southern Iberian Peninsula, and new insights into the evolutionary relationships within Belondiridae", "description": "<p>A new belondirid, dorylaimid taxon, Oleaxonchium olearumgen. et sp. nov., collected from an olive grove in the Andalusia region of Spain, is characterised, including its morphological description, morphometrics, SEM study, and molecular (18S-, 28S-rDNA) analyses. The new genus displays a unique combination of traits that distinguishes it from its closest genera: a rectangular lip region with sclerotized margins when observed in lateral view and visibly hexagonal in face view under SEM; a comparatively long cheilostom with thickened walls at its anterior part; a short isthmus-like section separating both pharyngeal regions; a mono-opistho-ovarian didelphic female genital system without pars refringens vaginae; and a short and rounded tail. The new species is characterised by its 2.44\uffe2\uff80\uff932.87 mm long body, lip region 7\uffe2\uff80\uff937.5 \uffc2\uffb5m wide, odontostyle 10\uffe2\uff80\uff9310.5 \uffc2\uffb5m long, neck 723\uffe2\uff80\uff93973 \uffc2\uffb5m long, pharyngeal expansion occupying 63\uffe2\uff80\uff9372% of the total neck length, female anterior genital branch 4\uffe2\uff80\uff936% of body length, tripartite posterior uterus 1.9\uffe2\uff80\uff932.6 body diameters long, with a short intermediate section bearing sclerotized elements, vulva (V = 58\uffe2\uff80\uff9361) a transverse slit, caudal region 29\uffe2\uff80\uff9335 \uffc2\uffb5m long (c = 74\uffe2\uff80\uff9389, c\uffe2\uff80\uff99 = 0.9\uffe2\uff80\uff931.1), and male unknown. As derived from an integrative approach combining morphological and molecular data, the new genus is close to Metaxonchium, the polyphyly of Belondiridae is confirmed, and support is provided in favour of the monophyly of Axonchiinae.</p", "keywords": ["0301 basic medicine", "0303 health sciences", "Nematoda", "18S-", "QH301-705.5", "Dorylaimida", "Belondiroidea", "phylogeny", "Biota", "new taxa", "Enoplea", "taxonomy", "03 medical and health sciences", "28S-rDNA", "morphology", "SEM", "Animalia", "Dorylaimia", "Belondiridae", "Biology (General)", "Dorylaimina"]}, "links": [{"href": "https://doi.org/10.3897/zse.100.128096"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Zoosystematics%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3897/zse.100.128096", "name": "item", "description": "10.3897/zse.100.128096", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3897/zse.100.128096"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-07-25T00:00:00Z"}}, {"id": "10.5061/dryad.h781v", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:28Z", "type": "Dataset", "title": "Data from: The microbially-mediated soil organic carbon loss under degenerative succession in an alpine meadow", "description": "unspecifiedMicrobial community and  network of meadow alpine soil by Illumina sequencingThe Qinghai-Tibet  Plateau is the highest and the largest low-latitude plateau in the world,  and also it is an extremely sensitive region to the impact of global  warming and environmental changes. The alpine meadow, widely distributed  on the Tibetan Plateau, occupies over 40% of the Qinghai-Tibetan Plateau  area and plays a critical role in regional sustainable development,  biodiversity and water resource conservation. The alpine meadow also was a  large soil organic-carbon pool.In recently decades, succession and  degradation were gradually occurring between different alpine meadow  types, such as alpine meadow might appear in the alpine steppe meadow  region according to years of field investigation which could be the  consequences of the climate warming and anthropogenic activities. The aims  of our study were to determine the effect of degenerated succession from  alpine meadow (AM) to alpine steppe meadow (ASM) on soil organic carbon  and soil microbial community structure.The archived files included one OTU  table generated from the 16S rRNA gene sequencing data, as well as the  input and output files for the network analyses.Dryad data  deposit.7z", "keywords": ["2. Zero hunger", "soil organic carbon", "16S rDNA sequencing", "ecological function", "13. Climate action", "soil microbes", "Microbial community", "15. Life on land", "Land Cover Change", "6. Clean water", "Metagenomic analysis", "12. Responsible consumption"], "contacts": [{"organization": "Zhang, Yuguang, Liu, Xiao, Cong, Jing, Lu, Hui, Sheng, Yuyu, Wang, Xiulei, Li, Diqiang, Liu, Xueduan, Yin, Huaqun, Zhou, Jizhong, Deng, Ye,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.h781v"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.h781v", "name": "item", "description": "10.5061/dryad.h781v", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.h781v"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-04-19T00:00:00Z"}}, {"id": "10.5281/zenodo.14190971", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:23:39Z", "type": "Journal Article", "created": "2024-07-25", "title": "Oleaxonchium olearum gen. et sp. nov. (Nematoda, Dorylaimida) associated with an olive grove in the southern Iberian Peninsula, and new insights into the evolutionary relationships within Belondiridae", "description": "<p>A new belondirid, dorylaimid taxon, Oleaxonchium olearumgen. et sp. nov., collected from an olive grove in the Andalusia region of Spain, is characterised, including its morphological description, morphometrics, SEM study, and molecular (18S-, 28S-rDNA) analyses. The new genus displays a unique combination of traits that distinguishes it from its closest genera: a rectangular lip region with sclerotized margins when observed in lateral view and visibly hexagonal in face view under SEM; a comparatively long cheilostom with thickened walls at its anterior part; a short isthmus-like section separating both pharyngeal regions; a mono-opistho-ovarian didelphic female genital system without pars refringens vaginae; and a short and rounded tail. The new species is characterised by its 2.44\uffe2\uff80\uff932.87 mm long body, lip region 7\uffe2\uff80\uff937.5 \uffc2\uffb5m wide, odontostyle 10\uffe2\uff80\uff9310.5 \uffc2\uffb5m long, neck 723\uffe2\uff80\uff93973 \uffc2\uffb5m long, pharyngeal expansion occupying 63\uffe2\uff80\uff9372% of the total neck length, female anterior genital branch 4\uffe2\uff80\uff936% of body length, tripartite posterior uterus 1.9\uffe2\uff80\uff932.6 body diameters long, with a short intermediate section bearing sclerotized elements, vulva (V = 58\uffe2\uff80\uff9361) a transverse slit, caudal region 29\uffe2\uff80\uff9335 \uffc2\uffb5m long (c = 74\uffe2\uff80\uff9389, c\uffe2\uff80\uff99 = 0.9\uffe2\uff80\uff931.1), and male unknown. As derived from an integrative approach combining morphological and molecular data, the new genus is close to Metaxonchium, the polyphyly of Belondiridae is confirmed, and support is provided in favour of the monophyly of Axonchiinae.</p", "keywords": ["0301 basic medicine", "taxonomy", "0303 health sciences", "03 medical and health sciences", "18S-", "28S-rDNA", "QH301-705.5", "morphology", "SEM", "Biology (General)", "phylogeny", "new taxa"]}, "links": [{"href": "https://doi.org/10.5281/zenodo.14190971"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Zoosystematics%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.14190971", "name": "item", "description": "10.5281/zenodo.14190971", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.14190971"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-07-25T00:00:00Z"}}, {"id": "oai:DiVA.org:mdh-42594", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-30T16:35:05Z", "type": "Report", "title": "V\u00e5rdande p\u00e5 akutmottagning : En systematisk litteraturstudie om sjuksk\u00f6terskors upplevelser av att v\u00e5rda p\u00e5 akutmottagning", "keywords": ["Omv\u00e5rdnad", "Nursing"], "contacts": [{"organization": "Lundblad, Matilda, Aspgren Kreivi, Sara,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/oai:DiVA.org:mdh-42594"}, {"rel": "self", "type": "application/geo+json", "title": "oai:DiVA.org:mdh-42594", "name": "item", "description": "oai:DiVA.org:mdh-42594", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/oai:DiVA.org:mdh-42594"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-01-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rDNA&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rDNA&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rDNA&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rDNA&offset=7", "hreflang": "en-US"}], "numberMatched": 7, "numberReturned": 7, "distributedFeatures": [], "timeStamp": "2026-05-30T23:14:47.635903Z"}