{"type": "FeatureCollection", "features": [{"id": "10.1016/j.apsoil.2022.104649", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:15:59Z", "type": "Journal Article", "created": "2022-08-30", "title": "Effects of microplastics on common bean rhizosphere bacterial communities", "description": "Microplastic pollution in terrestrial ecosystems is a growing concern due to its potential influences on soil properties and crop growth. Little is known about the effects of microplastics on the microbiome in the rhizosphere. Here, we studied the effects of two types of microplastics (MPs), low density polyethylene (LDPE-MPs) and biodegradable microplastic (Bio-MPs) of poly-butylene-adipate-co-terephthalate (PBAT) mixed with polylactic acid (PLA), on rhizosphere bacterial communities of Phaseolus vulgaris at doses of 0.5 %, 1.0 % and 2.5 % (w/w, dry weight ratio between MPs and soil). Bio-MPs and LDPE-MPs showed significant higher \u03b1-diversity (Chao 1, ACE, Shannon and Simpson) than control. For each type of microplastic material, 2.5 % of LDPE-MPs and Bio-MPs showed lowest \u03b1-diversity as compared to doses of 0.5 % and 1.0 %, indicating 2.5 % dose of MPs might pose selective effect on rhizosphere bacterial communities. \u03b2-Diversity of 1.0 % and 2.5 % Bio-MPs were distinctive from the control and other treatments. Microplastics also affected the relative abundance at family level, i.e. as compared to control, Comamonadaceae was higher in all the MPs treatments, Rhizobiaceae was highest in 2.5 % LDPE-MPs and lowest in 2.5 % Bio-MPs. LefSe results showed, as compared to control, Bio-MPs induced more indictive taxa than LDPE-MPs. Our findings evidenced that LDPE-MPs and Bio-MPs exerted profound effects on rhizosphere bacterial communities, and these effects might have far-reaching effects on soil nutrient cycling and plant health in agroecosystems.", "keywords": ["2. Zero hunger", "0301 basic medicine", "03 medical and health sciences", "Low density polyethylene plastic", "13. Climate action", "Microplastics", "16S rRNA", "15. Life on land", "Biodegradable plastic", "Rhizosphere bacterial community", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2022.104649"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2022.104649", "name": "item", "description": "10.1016/j.apsoil.2022.104649", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2022.104649"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10.1111/nph.19112", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:49Z", "type": "Journal Article", "created": "2023-06-29", "title": "Gold\u2010FISH enables targeted NanoSIMS analysis of plant\u2010associated bacteria", "description": "Summary<p> <p>Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of in\uffc2\uffa0situ evidence of bacterial activity.</p> <p>To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold\uffe2\uff80\uff90based in\uffc2\uffa0situ hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity.</p> <p>We incubated Kosakonia strain DS\uffe2\uff80\uff901\uffe2\uff80\uff90associated, gnotobiotically grown rice plants with 15N\uffe2\uff80\uff93N2 gas to detect in\uffc2\uffa0situ N2 fixation activity. Bacterial cells along the rhizoplane showed\uffc2\uffa0heterogeneous patterns of 15N enrichment, ranging from the natural isotope abundance levels up to 12.07 at% 15N (average and median of 3.36 and 2.85 at% 15N, respectively, n\uffe2\uff80\uff89=\uffe2\uff80\uff89697 cells).</p> <p>The presented correlative optical and chemical imaging analysis is applicable to a broad range of studies investigating plant\uffe2\uff80\uff93microbe interactions. For example, it enables verification of the in\uffc2\uffa0situ metabolic activity of host\uffe2\uff80\uff90associated commercialized strains or plant growth\uffe2\uff80\uff90promoting bacteria, thereby disentangling their role in plant nutrition. Such data facilitate the design of plant\uffe2\uff80\uff93microbe combinations for improvement of crop management.</p> </p", "keywords": ["2. Zero hunger", "106022 Mikrobiologie", "rhizosphere bacteria", "Bacteria", "plant growth-promoting bacteria", "plant\u2013microbe interaction", "Research", "Oryza", "biological nitrogen fixation", "Plants", "microbial activity", "in\u00a0situ hybridization", "Plant Roots", "106026 \u00d6kosystemforschung", "Rhizosphere", "106022 Microbiology", "NanoSIMS", "in situ hybridization", "106026 Ecosystem research", "In situ hybridization", "In Situ Hybridization", "Soil Microbiology", "plant-microbe interaction"]}, "links": [{"href": "https://doi.org/10.1111/nph.19112"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.19112", "name": "item", "description": "10.1111/nph.19112", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.19112"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-06-28T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.02694", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:21:36Z", "type": "Journal Article", "created": "2019-12-10", "title": "The Impact of Soil-Applied Biochars From Different Vegetal Feedstocks on Durum Wheat Plant Performance and Rhizospheric Bacterial Microbiota in Low Metal-Contaminated Soil", "description": "Biochar shapes the soil environment and plant growth. Nevertheless, the mechanisms associated with an improved plant biomass and soil microbiome in low metal-contaminated soils are still unclear. In this study, the influence of biochar on soil physico-chemical properties, plant performance, and rhizosphere microbiota in durum wheat was investigated at the above- and belowground levels. Two kinds of biochar from different feedstocks (wood chips and wheat straw pellets) and two Italian durum wheat varieties, Duilio and Marco Aurelio, were analyzed in a greenhouse using a low-nutrient gleyic fluvisol containing a very small amount of Pb and Zn. Four different treatments were performed: soil-only control (C), soil amended with woody biochar equilibrated with nutrient solution (B1+) and non-activated (B1-), and soil amended with non-activated (B2-) wheat straw biochar. Seven weeks after seed germination, (1) the physico-chemical properties of soil, biochars, and mixtures were assessed; (2) the fresh and dry weight of aboveground plant tissues and roots and other morphometric traits were measured; and (3) metabarcoding of the 16S rRNA bacterial gene was performed on rhizosphere soil samples. The results showed that the biochar from wheat straw had stronger impact on both durum varieties, with higher electrical conductivity, higher levels of available K and Na, and a substantial increase of dissolved Na+, K+, and Cl- ions in pore water. Generally, biochar amendment decreased Zn availability for the plants. In addition, biochar improved plant growth in the early growth stage, and the more positive effect was achieved by combining wheat straw biochar with Marco Aurelio. Rhizosphere bacterial microbiota showed variation in alpha diversity only due to treatment; on the other hand, the differential analysis showed consistent variation among samples with significant effects on amplicon sequence variant (ASV) abundance due to the specific biochar treatment as well as the genotype. The pure B1-, due to its scarce nutrient content with respect to the richer types (B1+ and B2-), had a negative impact on microbiota richness. Our study highlights that an appropriate combination of biochar feedstock and crop species may lead to superior yield.", "keywords": ["2. Zero hunger", "low-metal contaminated soil", "biochar; durum wheat; low-metal contaminated soil; rhizosphere bacterial microbiome; vegetal feedstock", "durum wheat", "vegetal feedstock", "04 agricultural and veterinary sciences", "15. Life on land", "Microbiology", "QR1-502", "6. Clean water", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "biochar", "rhizosphere bacterial microbiome"]}, "links": [{"href": "https://flore.unifi.it/bitstream/2158/1215663/1/Latini_et_al-2019.pdf"}, {"href": "https://doi.org/10.3389/fmicb.2019.02694"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.02694", "name": "item", "description": "10.3389/fmicb.2019.02694", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.02694"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-10T00:00:00Z"}}, {"id": "11353/10.2037573", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:26:12Z", "type": "Journal Article", "created": "2023-06-29", "title": "Gold\u2010FISH enables targeted NanoSIMS analysis of plant\u2010associated bacteria", "description": "Summary<p> <p>Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of in\uffc2\uffa0situ evidence of bacterial activity.</p> <p>To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold\uffe2\uff80\uff90based in\uffc2\uffa0situ hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity.</p> <p>We incubated Kosakonia strain DS\uffe2\uff80\uff901\uffe2\uff80\uff90associated, gnotobiotically grown rice plants with 15N\uffe2\uff80\uff93N2 gas to detect in\uffc2\uffa0situ N2 fixation activity. Bacterial cells along the rhizoplane showed\uffc2\uffa0heterogeneous patterns of 15N enrichment, ranging from the natural isotope abundance levels up to 12.07 at% 15N (average and median of 3.36 and 2.85 at% 15N, respectively, n\uffe2\uff80\uff89=\uffe2\uff80\uff89697 cells).</p> <p>The presented correlative optical and chemical imaging analysis is applicable to a broad range of studies investigating plant\uffe2\uff80\uff93microbe interactions. For example, it enables verification of the in\uffc2\uffa0situ metabolic activity of host\uffe2\uff80\uff90associated commercialized strains or plant growth\uffe2\uff80\uff90promoting bacteria, thereby disentangling their role in plant nutrition. Such data facilitate the design of plant\uffe2\uff80\uff93microbe combinations for improvement of crop management.</p> </p", "keywords": ["2. Zero hunger", "106022 Mikrobiologie", "rhizosphere bacteria", "Bacteria", "plant growth-promoting bacteria", "plant\u2013microbe interaction", "Research", "Oryza", "biological nitrogen fixation", "Plants", "microbial activity", "in\u00a0situ hybridization", "Plant Roots", "106026 \u00d6kosystemforschung", "Rhizosphere", "106022 Microbiology", "NanoSIMS", "in situ hybridization", "106026 Ecosystem research", "In situ hybridization", "In Situ Hybridization", "Soil Microbiology", "plant-microbe interaction"]}, "links": [{"href": "https://doi.org/11353/10.2037573"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11353/10.2037573", "name": "item", "description": "11353/10.2037573", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11353/10.2037573"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-06-28T00:00:00Z"}}, {"id": "2158/1215663", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:26:58Z", "type": "Journal Article", "created": "2019-12-10", "title": "The Impact of Soil-Applied Biochars From Different Vegetal Feedstocks on Durum Wheat Plant Performance and Rhizospheric Bacterial Microbiota in Low Metal-Contaminated Soil", "description": "Biochar shapes the soil environment and plant growth. Nevertheless, the mechanisms associated with an improved plant biomass and soil microbiome in low metal-contaminated soils are still unclear. In this study, the influence of biochar on soil physico-chemical properties, plant performance, and rhizosphere microbiota in durum wheat was investigated at the above- and belowground levels. Two kinds of biochar from different feedstocks (wood chips and wheat straw pellets) and two Italian durum wheat varieties, Duilio and Marco Aurelio, were analyzed in a greenhouse using a low-nutrient gleyic fluvisol containing a very small amount of Pb and Zn. Four different treatments were performed: soil-only control (C), soil amended with woody biochar equilibrated with nutrient solution (B1+) and non-activated (B1-), and soil amended with non-activated (B2-) wheat straw biochar. Seven weeks after seed germination, (1) the physico-chemical properties of soil, biochars, and mixtures were assessed; (2) the fresh and dry weight of aboveground plant tissues and roots and other morphometric traits were measured; and (3) metabarcoding of the 16S rRNA bacterial gene was performed on rhizosphere soil samples. The results showed that the biochar from wheat straw had stronger impact on both durum varieties, with higher electrical conductivity, higher levels of available K and Na, and a substantial increase of dissolved Na+, K+, and Cl- ions in pore water. Generally, biochar amendment decreased Zn availability for the plants. In addition, biochar improved plant growth in the early growth stage, and the more positive effect was achieved by combining wheat straw biochar with Marco Aurelio. Rhizosphere bacterial microbiota showed variation in alpha diversity only due to treatment; on the other hand, the differential analysis showed consistent variation among samples with significant effects on amplicon sequence variant (ASV) abundance due to the specific biochar treatment as well as the genotype. The pure B1-, due to its scarce nutrient content with respect to the richer types (B1+ and B2-), had a negative impact on microbiota richness. Our study highlights that an appropriate combination of biochar feedstock and crop species may lead to superior yield.", "keywords": ["2. Zero hunger", "low-metal contaminated soil", "biochar; durum wheat; low-metal contaminated soil; rhizosphere bacterial microbiome; vegetal feedstock", "durum wheat", "vegetal feedstock", "04 agricultural and veterinary sciences", "15. Life on land", "Microbiology", "QR1-502", "6. Clean water", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "biochar", "rhizosphere bacterial microbiome"]}, "links": [{"href": "https://flore.unifi.it/bitstream/2158/1215663/1/Latini_et_al-2019.pdf"}, {"href": "https://doi.org/2158/1215663"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2158/1215663", "name": "item", "description": "2158/1215663", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2158/1215663"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-10T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rhizosphere+bacteria&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rhizosphere+bacteria&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rhizosphere+bacteria&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=rhizosphere+bacteria&offset=5", "hreflang": "en-US"}], "numberMatched": 5, "numberReturned": 5, "distributedFeatures": [], "timeStamp": "2026-07-28T13:11:19.985298Z"}