{"type": "FeatureCollection", "features": [{"id": "10.1016/j.envexpbot.2020.104095", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:16:36Z", "type": "Journal Article", "created": "2020-04-25", "title": "Alternation of wet and dry sides during partial rootzone drying irrigation enhances leaf ethylene evolution", "description": "Soil drying increases endogenous ABA and ACC concentrations in planta, but how these compounds interact to regulate stomatal responses to soil drying and re-watering is still unclear. To determine the temporal dynamics and physiological significance of root, xylem and leaf ABA and ACC concentrations in response to deficit irrigation (DI) or partial rootzone drying (PRD-F) and re-watering, these variables were measured in plants exposed to similar whole pot soil water contents. Both DI and PRD-F plants received only a fraction of the irrigation supplied to well-watered (WW) plants, either to all (DI) or part (PRD-F) of the rootzone of plants grown in split-pots. Both DI and PRD-F induced partial stomatal closure, increased root ABA and ACC accumulation consistent with local soil water content, but did not affect xylem or leaf concentrations of these compounds compared to WW plants. Two hours after re-watering all (DI-RW) or part of the rootzone (PRD-A) to the same soil water content, stomatal conductance returned to WW values or further decreased respectively. Re-watering the whole rootzone had no effect on xylem and leaf ABA and ACC concentrations, while re-watering the dry side of the pot in PRD plants had no effect on xylem and leaf ABA concentrations but increased xylem and leaf ACC concentrations and leaf ethylene evolution. Leaf water potential was similar between all irrigation treatments, with stomatal conductance declining as xylem ABA concentrations and leaf ACC concentrations increased. Prior to re-watering PRD plants, accounting for the spatial differences in soil water uptake best explained variation in xylem ACC concentration suggesting root-to-shoot ACC signalling, but this model did not account for variation in xylem ACC concentration after re-watering the dry side of PRD plants. Thus local (foliar) and long-distance (root-to-shoot) variation in ACC status both seem important in regulating the temporal dynamics of foliar ethylene evolution in plants exposed to PRD.", "keywords": ["0106 biological sciences", "Irrigation", "Stomatal conductance", "Root-to-shoot signalling", "Ethylene", "Physiological significance", "Deficit irrigation", "Plant Science", "Leaf water", "F06 Irrigation", "01 natural sciences", "ACC", "Ecology", " Evolution", " Behavior and Systematics", "580", "2. Zero hunger", "Xylem", "15. Life on land", "F60 Plant physiology and biochemistry", "6. Clean water", "Horticulture", "13. Climate action", "Soil water", "Agronomy and Crop Science", "Soil moisture heterogeneity", "Partial rootzone drying"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/144510/1/Juan_EEB_Manuscript_final.pdf"}, {"href": "https://doi.org/10.1016/j.envexpbot.2020.104095"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20and%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envexpbot.2020.104095", "name": "item", "description": "10.1016/j.envexpbot.2020.104095", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envexpbot.2020.104095"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-01T00:00:00Z"}}, {"id": "10.1016/j.agwat.2018.06.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:16:06Z", "type": "Journal Article", "created": "2018-06-18", "title": "Estimating the water budget components of irrigated crops: Combining the FAO-56 dual crop coefficient with surface temperature and vegetation index data", "description": "Abstract The FAO-56 dual crop coefficient (FAO-2Kc) model has been extensively used at the field scale to estimate the crop water requirements by means of the simulated evapotranspiration (ET) and its two components evaporation (E) and transpiration (T). Given that the main limitation of FAO-2Kc for operational irrigation management over large areas is the unavailability (over most irrigated areas) of irrigation data, this study investigates the feasibility 1) to constrain the FAO-2Kc ET from LST and VI data, 2) to retrieve irrigation amounts and dates from LST and VI data and 3) to estimate the root-zone soil moisture (RZSM) at the daily scale. In practice, the vegetation and soil temperatures retrieved from LST/VI data are used to estimate the FAO-2Kc vegetation stress coefficient (Ks) and soil evaporation reduction coefficient (Kr), respectively. The modeling and remote sensing combined approach is tested over a wheat crop field in central Morocco, and results are evaluated in terms of ET, irrigation and RZSM estimates. ET is estimated with a RMSE of 0.68\u202fmm day-1 compared to 0.84\u202fmm day-1 for the standard (without using LST data) FAO-2Kc based on tabulated values for the parameters. The total irrigation depth (67\u202fmm) is correctly estimated and is very close to the actual effective irrigation (69.8\u202fmm) applied by the farmer. Daily RZSM is estimated with an R2 value of 0.68 (0.42) and a RMSE value of 0.034 (0.061) m3 m-3 by forcing FAO-2Kc using the retrieved irrigation (from LST-derived estimates and precipitation only). Since spaceborne LST data are currently not available at both high-spatial and high-temporal resolution, a sensitivity analysis is finally undertaken to assess the potential and applicability of the proposed methodology to temporally-sparse thermal data.", "keywords": ["FAO-56", "0106 biological sciences", "2. Zero hunger", "550", "Evapotranspiration", "[SDE.IE]Environmental Sciences/Environmental Engineering", "Root-zone soil moisture", "[SDV.SA.STA] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture", "Root-Zone Soil Moisture", "Surface Temperature", "[INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation", "01 natural sciences", "6. Clean water", "Surface temperature", "[SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture", "[INFO.INFO-MO] Computer Science [cs]/Modeling and Simulation", "[SDE.IE] Environmental Sciences/Environmental Engineering", "Irrigation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.agwat.2018.06.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agricultural%20Water%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agwat.2018.06.014", "name": "item", "description": "10.1016/j.agwat.2018.06.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agwat.2018.06.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-01T00:00:00Z"}}, {"id": "10.1002/jpln.202000183", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:14:39Z", "type": "Journal Article", "created": "2020-10-09", "title": "Effects of water deficit and nitrogen application on leaf gas exchange, phytohormone signaling, biomass and water use efficiency of oat plants", "description": "Abstract

Background: Water and nitrogen (N) are essential resources influencing plant growth and yield. To improve their efficiencies in crop production is challenging because the physiological mechanisms of water and N coupling and their interactive effect on crop water use efficiency (WUE) are not well understood yet.

Aim: The aim of this study was to investigate the physiological responses and phytohormones signaling in oats in response to soil water status and N supply under fertigation, to explore the mechanisms regulating plant growth and WUE.

Methods: Oat plants were subjected to the factorial combination of three soil moisture regimes (50, 70, and 90% of soil water holding capacity, SWHC) and three N levels (fertilized with 74, 149, and 298 mg kg\uffe2\uff88\uff921).

Results: The stomatal conductance (gs) was significantly decreased by soil water deficit, and also by the highest N level, whereas photosynthesis rate (An) was unaffected by neither water nor N. Consequently, intrinsic WUE (WUEint, An/gs) was highest under reduced irrigation and high N fertilization. This effect at stomatal level was affirmed by responses in whole plant WUE (WUEb), which was positively correlated with shoot \uffce\uffb413C. A positive correlation between \uffce\uffb418O and \uffce\uffb413C in shoots further indicated that decreases of gs rather than changes in An contributed to the enhanced WUE.

Conclusion: Moderate soil water deficit and sufficient N supply is recommended for saving irrigation water and improving WUE on fertigated oat plants without compromising biomass accumulation to any large extent.9\u00a0\u03bcm (equivalent pore diameter), in accordance with lower \u03c1 b . Available water capacity was greater with NT than with RT and MT. Higher SWC under conservation tillage systems (NT, NTSB and RT) than under MT was attributed mainly to greater AWC and to the mulching effect of crop residues. Crop yield in the driest year of the 5-year period was lowest under MT whereas no differences among treatments were found over the 5-year period. Stubble burning did not affect AWC nor barley yield. Tillage had a greater impact on soil properties and on crop yield than crop residue management.", "keywords": ["2. Zero hunger", "pore-size distribution", "no-tillage", "crop management", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "storage", "conservation tillage", "systems", "0401 agriculture", " forestry", " and fisheries", "root-growth", "physical-properties", "grain-yield", "organic-matter"]}, "links": [{"href": "https://doi.org/10.1016/j.still.2005.02.028"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20and%20Tillage%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.still.2005.02.028", "name": "item", "description": "10.1016/j.still.2005.02.028", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.still.2005.02.028"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-05-01T00:00:00Z"}}, {"id": "10.1016/s1002-0160(21)60061-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:17:57Z", "type": "Journal Article", "created": "2021-12-08", "title": "Rhizosphere microbiomes can regulate plant drought tolerance", "description": "Open AccessPeer reviewed", "keywords": ["0301 basic medicine", "2. Zero hunger", "Drought stress", "0303 health sciences", "Root-microbe association", "15. Life on land", "Biota", "6. Clean water", "12. Responsible consumption", "Phytohormones", "03 medical and health sciences", "Phytohormone", "13. Climate action", "Metabolites", "Plant growth"], "contacts": [{"organization": "ASLAM, Mehtab Muhammad, OKAL, Eyalira J., IDRIS, Aisha Lawan, QIAN, Zhang, XU, Weifeng, KARANJA, Joseph K., WANI, Shabir H., YUAN, Wei,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/s1002-0160(21)60061-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Pedosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/s1002-0160(21)60061-9", "name": "item", "description": "10.1016/s1002-0160(21)60061-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/s1002-0160(21)60061-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "10.1073/pnas.2109176118", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:18:46Z", "type": "Journal Article", "created": "2021-02-13", "title": "Plant-environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere", "description": "Abstract

Our understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.

", "keywords": ["0301 basic medicine", "570", "Microscopy", "Silicon", "0303 health sciences", "Temperature", "root-microbe interactions", "Equipment Design", "Biological Sciences", "Environment", "15. Life on land", "Plant Roots", "630", "Fluorescence", "Soil", "03 medical and health sciences", "Seedlings", "Calibration", "Rhizosphere", "Image Processing", " Computer-Assisted", "environmental imaging", "rhizosphere", "Soil Microbiology", "Bacillus subtilis", "Lactuca"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/178939/18/e2109176118.full.pdf"}, {"href": "https://pnas.org/doi/pdf/10.1073/pnas.2109176118"}, {"href": "https://doi.org/10.1073/pnas.2109176118"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.2109176118", "name": "item", "description": "10.1073/pnas.2109176118", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.2109176118"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-13T00:00:00Z"}}, {"id": "10.1093/plcell/koac263", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:06Z", "type": "Journal Article", "created": "2022-08-26", "title": "Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress", "description": "Abstract

We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these questions include the need to better understand how plants detect water availability, temperature, salinity, and rising carbon dioxide (CO2) levels; how environmental signals interface with endogenous signaling and development (e.g. circadian clock and flowering time); and how this integrated signaling controls downstream responses (e.g. stomatal regulation, proline metabolism, and growth versus defense balance). The plasma membrane comes up frequently as a site of key signaling and transport events (e.g. mechanosensing and lipid-derived signaling, aquaporins). Adaptation to water extremes and rising CO2 affects hydraulic architecture and transpiration, as well as root and shoot growth and morphology, in ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution and crop resilience in the face of changing and increasingly unpredictable environments. Exploration of plant diversity within and among species can help us know which of these tradeoffs represent fundamental limits and which ones can be circumvented by bringing new trait combinations together. Better defining what constitutes beneficial stress resistance in different contexts and making connections between genes and phenotypes, and between laboratory and field observations, are overarching challenges.Soil salinity, in both natural and managed environments, is highly heterogeneous, and understanding how plants respond to this spatiotemporal heterogeneity is increasingly important for sustainable agriculture in the era of global climate change. While the vast majority of research on crop response to salinity utilizes homogeneous saline conditions, a much smaller, but important, effort has been made in the past decade to understand plant molecular and physiological responses to heterogeneous salinity mainly by using split-root studies. These studies have begun to unravel how plants compensate for water/nutrient deprivation and limit salt stress by optimizing root-foraging in the most favourable parts of the soil.

Scope

This paper provides an overview of the patterns of salinity heterogeneity in rain-fed and irrigated systems. We then discuss results from split-root studies and the recent progress in understanding the physiological and molecular mechanisms regulating plant responses to heterogeneous root-zone salinity and nutrient conditions. We focus on mechanisms by which plants (salt/nutrient sensing, root-shoot signalling and water uptake) could optimize the use of less-saline patches within the root-zone, thereby enhancing growth under heterogeneous soil salinity conditions. Finally, we place these findings in the context of defining future research priorities, possible irrigation management and crop breeding opportunities to improve productivity from salt-affected lands.

", "keywords": ["Nutrient heterogeneity", "Water uptake", "Root-to-shoot signalling", "Salinity", "550", "Plant Biology & Botany", "Plant Biology", "Irrigation; nutrient heterogeneity; phytohormones; root foraging; root-to-shoot signalling; salt sensing; stomatal conductance; water uptake", "Stomatal conductance", "Salt sensing", "Plant Roots", "630", "12. Responsible consumption", "root foraging", "Soil", "Irrigation", "salt sensing", "Root foraging", "580", "2. Zero hunger", "Ecology", "Forestry Sciences", "Research", "nutrient heterogeneity", "Water", "15. Life on land", "6. Clean water", "root-to-shoot signalling", "phytohormones", "Phytohormones", "stomatal conductance", "13. Climate action", "Zero Hunger", "water uptake"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/166913/1/21783_2_merged_1643798007.pdf"}, {"href": "https://academic.oup.com/aob/article-pdf/129/5/499/43374309/mcac022.pdf"}, {"href": "https://escholarship.org/content/qt7t32v7cc/qt7t32v7cc.pdf"}, {"href": "https://doi.org/10.1093/aob/mcac022"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Annals%20of%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/aob/mcac022", "name": "item", "description": "10.1093/aob/mcac022", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/aob/mcac022"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-16T00:00:00Z"}}, {"id": "10.1093/jxb/erx494", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:05Z", "type": "Journal Article", "created": "2018-01-12", "title": "Strigolactones: mediators of osmotic stress responses with a potential for agrochemical manipulation of crop resilience", "description": "After quickly touching upon general aspects of strigolactone biology and functions, including structure, synthesis, and perception, this review focuses on the role and regulation of the strigolactone pathway during osmotic stress, in light of the most recent research developments. We discuss available data on organ-specific dynamics of strigolactone synthesis and interaction with abscisic acid in the acclimatization response, with emphasis on the ecophysiological implications of the effects on the stomatal closure process. We highlight the importance of considering roots and shoots separately as well as combined versus individual stress treatments; and of performing reciprocal grafting experiments to work out organ contributions and long-distance signalling events and components under more realistic conditions. Finally, we elaborate on the question of if and how synthetic or natural strigolactones, alone or in combination with crop management strategies such as grafting, hold potential to maximize crop resilience to abiotic stresses.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Abscisic acid", " Drought", " Hormone cross-talk", " Osmotic stress", " Resilience", " Root-shoot communication", " Stomata closure", " Strigolactones", "Abscisic acid; Drought; Hormone crosstalk; Osmotic stress; Resilience; Root-shoot communication; Stomatal closure; Strigolactones; Abscisic Acid; Crops", " Agricultural; Lactones; Plant Growth Regulators; Plant Roots; Plant Shoots; Osmoregulation; Plant Physiological Phenomena", "15. Life on land", "Plant Roots", "Lactones", "03 medical and health sciences", "Osmoregulation", "Plant Growth Regulators", "Plant Physiological Phenomena", "Plant Shoots", "Abscisic Acid"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/898419/2/erx494.pdf"}, {"href": "https://iris.unito.it/bitstream/2318/1661457/1/JEXBOT-2017-212340v2-Cardinale.pdf"}, {"href": "http://academic.oup.com/jxb/article-pdf/69/9/2291/24701402/erx494.pdf"}, {"href": "https://doi.org/10.1093/jxb/erx494"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jxb/erx494", "name": "item", "description": "10.1093/jxb/erx494", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jxb/erx494"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-15T00:00:00Z"}}, {"id": "10.1101/2021.02.13.430456", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:11Z", "type": "Journal Article", "created": "2021-02-13", "title": "Plant-environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere", "description": "Abstract

Our understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.The evaporation of water from land into the atmosphere is a key component of the hydrological cycle. Accurate estimates of this flux are essential for proper water management and irrigation scheduling. However, continuous and qualitative information on land evaporation is currently not available at the required spatio-temporal scales for agricultural applications and regional-scale water management. Here, we apply the Global Land Evaporation Amsterdam Model (GLEAM) at 100 m spatial resolution and daily time steps to provide estimates of land evaporation over The Netherlands, Flanders, and western Germany for the period 2013\uffe2\uff80\uff932017. By making extensive use of microwave-based geophysical observations, we are able to provide data under all weather conditions. The soil moisture estimates from GLEAM at high resolution compare well with in situ measurements of surface soil moisture, resulting in a median temporal correlation coefficient of 0.76 across 29 sites. Estimates of terrestrial evaporation are also evaluated using in situ eddy-covariance measurements from five sites, and compared to estimates from the coarse-scale GLEAM v3.2b, land evaporation from the Satellite Application Facility on Land Surface Analysis (LSA-SAF), and reference grass evaporation based on Makkink\uffe2\uff80\uff99s equation. All datasets compare similarly with in situ measurements and differences in the temporal statistics are small, with correlation coefficients against in situ data ranging from 0.65 to 0.95, depending on the site. Evaporation estimates from GLEAM-HR are typically bounded by the high values of the Makkink evaporation and the low values from LSA-SAF. While GLEAM-HR and LSA-SAF show the highest spatial detail, their geographical patterns diverge strongly due to differences in model assumptions, model parameterizations, and forcing data. The separate consideration of rainfall interception loss by tall vegetation in GLEAM-HR is a key cause of this divergence: while LSA-SAF reports maximum annual evaporation volumes in the Green Heart of The Netherlands, an area dominated by shrubs and grasses, GLEAM-HR shows its maximum in the national parks of the Veluwe and Heuvelrug, both densely-forested regions where rainfall interception loss is a dominant process. The pioneering dataset presented here is unique in that it provides observational-based estimates at high resolution under all weather conditions, and represents a viable alternative to traditional visible and infrared models to retrieve evaporation at field scales.

", "keywords": ["microwave remote sensing", "EVAPOTRANSPIRATION", "WACMOS-ET PROJECT", "Science", "FLUXNET", "Q", "LSA-SAF", "15. Life on land", "01 natural sciences", "6. Clean water", "MODEL", "CARBON", "VARIABILITY", "terrestrial evaporation", "root-zone soil moisture", "13. Climate action", "Earth and Environmental Sciences", "SURFACE EVAPORATION", "GLOBAL DATABASE", "WATER", "SOIL-MOISTURE RETRIEVALS", "terrestrial evaporation; root-zone soil moisture; microwave remote sensing; GLEAM; LSA-SAF", "GLEAM", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://www.mdpi.com/2072-4292/10/11/1720/pdf"}, {"href": "https://doi.org/10.3390/rs10111720"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Remote%20Sensing", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/rs10111720", "name": "item", "description": "10.3390/rs10111720", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/rs10111720"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-10-31T00:00:00Z"}}, {"id": "2809041101", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:26:56Z", "type": "Journal Article", "created": "2018-06-18", "title": "Estimating the water budget components of irrigated crops: Combining the FAO-56 dual crop coefficient with surface temperature and vegetation index data", "description": "Abstract The FAO-56 dual crop coefficient (FAO-2Kc) model has been extensively used at the field scale to estimate the crop water requirements by means of the simulated evapotranspiration (ET) and its two components evaporation (E) and transpiration (T). Given that the main limitation of FAO-2Kc for operational irrigation management over large areas is the unavailability (over most irrigated areas) of irrigation data, this study investigates the feasibility 1) to constrain the FAO-2Kc ET from LST and VI data, 2) to retrieve irrigation amounts and dates from LST and VI data and 3) to estimate the root-zone soil moisture (RZSM) at the daily scale. In practice, the vegetation and soil temperatures retrieved from LST/VI data are used to estimate the FAO-2Kc vegetation stress coefficient (Ks) and soil evaporation reduction coefficient (Kr), respectively. The modeling and remote sensing combined approach is tested over a wheat crop field in central Morocco, and results are evaluated in terms of ET, irrigation and RZSM estimates. ET is estimated with a RMSE of 0.68\u202fmm day-1 compared to 0.84\u202fmm day-1 for the standard (without using LST data) FAO-2Kc based on tabulated values for the parameters. The total irrigation depth (67\u202fmm) is correctly estimated and is very close to the actual effective irrigation (69.8\u202fmm) applied by the farmer. Daily RZSM is estimated with an R2 value of 0.68 (0.42) and a RMSE value of 0.034 (0.061) m3 m-3 by forcing FAO-2Kc using the retrieved irrigation (from LST-derived estimates and precipitation only). Since spaceborne LST data are currently not available at both high-spatial and high-temporal resolution, a sensitivity analysis is finally undertaken to assess the potential and applicability of the proposed methodology to temporally-sparse thermal data.", "keywords": ["FAO-56", "0106 biological sciences", "2. Zero hunger", "550", "Evapotranspiration", "[SDE.IE]Environmental Sciences/Environmental Engineering", "Root-zone soil moisture", "[SDV.SA.STA] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture", "Root-Zone Soil Moisture", "Surface Temperature", "[INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation", "01 natural sciences", "6. Clean water", "Surface temperature", "[SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture", "[INFO.INFO-MO] Computer Science [cs]/Modeling and Simulation", "[SDE.IE] Environmental Sciences/Environmental Engineering", "Irrigation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/2809041101"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agricultural%20Water%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2809041101", "name": "item", "description": "2809041101", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2809041101"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-01T00:00:00Z"}}, {"id": "10.3390/w14081188", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:57Z", "type": "Journal Article", "created": "2022-04-10", "title": "Estimating Yield from NDVI, Weather Data, and Soil Water Depletion for Sugar Beet and Potato in Northern Belgium", "description": "

Crop-yield models based on vegetation indices such as the normalized difference vegetation index (NDVI) have been developed to monitor crop yield at higher spatial and temporal resolutions compared to agricultural statistical data. We evaluated the model performance of NDVI-based random forest models for sugar beet and potato farm yields in northern Belgium during 2016\u20132018. We also evaluated whether weather variables and root-zone soil water depletion during the growing season improved the model performance. The NDVI integral did not explain early and late potato yield variability and only partly explained sugar-beet yield variability. The NDVI series of early and late potato crops were not sensitive enough to yield affecting weather and soil water conditions. We found that water-saturated conditions early in the growing season and elevated temperatures late in the growing season explained a large part of the sugar-beet and late-potato yield variability. The NDVI integral in combination with monthly precipitation, maximum temperature, and root-zone soil water depletion during the growing season explained farm-scale sugar beet (R2 = 0.84, MSE = 48.8) and late potato (R2 = 0.56, MSE = 57.3) yield variability well from 2016 to 2018 in northern Belgium.

", "keywords": ["AquaCrop-OSPy", "STRESS", "root-zone soil water depletion; AquaCrop-OSPy; sugar beet; potato; crop yield; NDVI; Belgium; weather impact; random forest", "NDVI", "Environmental Sciences & Ecology", "root-zone soil water depletion", "01 natural sciences", "Belgium", "INDEX", "0105 earth and related environmental sciences", "2. Zero hunger", "Science & Technology", "PRODUCTIVITY", "CROP", "sugar beet", "weather impact", "04 agricultural and veterinary sciences", "crop yield", "WINTER-WHEAT", "15. Life on land", "MODEL", "Physical Sciences", "Water Resources", "potato", "0401 agriculture", " forestry", " and fisheries", "Life Sciences & Biomedicine", "Environmental Sciences", "random forest"]}, "links": [{"href": "http://www.mdpi.com/2073-4441/14/8/1188/pdf"}, {"href": "https://www.mdpi.com/2073-4441/14/8/1188/pdf"}, {"href": "https://doi.org/10.3390/w14081188"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/w14081188", "name": "item", "description": "10.3390/w14081188", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/w14081188"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-08T00:00:00Z"}}, {"id": "10.3389/fclim.2024.1344524", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:29Z", "type": "Journal Article", "created": "2024-07-29", "title": "Quantification of soil organic carbon: the challenge of biochar-induced spatial heterogeneity", "description": "Introduction

Soil organic carbon (SOC) content can vary significantly across a given plot. Therefore, a representative sampling is a prerequisite to obtain meaningful results from analysis and of utter importance when SOC quantification is used to quantify (temporary) carbon dioxide removal (CDR). However, certain management practices aiming to increase SOC further increase the level of heterogeneity and may challenge representative sampling schemes. This includes concentrated root-zone application of biochar, which immediately increases SOC with the input of biochar-C and may promote the local enrichment of non-biochar SOC over time.

Methods

Here, we used numerical modelling to quantify the number of single sampling points (soil cores) needed to achieve a representative sample of biochar-C and total SOC on a plot after application of biochar in rows, e.g., for growing vegetables, or in the circumference of trees in agroforestry systems.

Results

After row application of 5\uffe2\uff80\uff89t\uffe2\uff80\uff89ha\uffe2\uff88\uff921 biochar in soil with rather low SOC content (26\uffe2\uff80\uff89t\uffe2\uff80\uff89ha\uffe2\uff88\uff921), 140 soil cores (per 0.25\uffe2\uff80\uff89ha plot) where necessary to achieve representative sampling of C-stocks (\uffc2\uffb15% error) in 90% of the repeated sampling simulation cases. Compared to realistic and cost-effective soil sampling scenarios in agronomic practice, we conclude that concentrated root zone application of biochar makes representative sampling for quantification of SOC in soils with low baseline C-stocks virtually impossible.

Discussion

This finding calls into question the soil-sampling and SOC-analysis-based (\uffe2\uff80\uff9cresult-based\uffe2\uff80\uff9d) monitoring of SOC as a (temporary) CDR when biochar might have been applied. Considering the rapid scaling of biochar production and use in agriculture, this is a considerable challenge for SOC certification. Instead, action-based incentives, rewarding farmers for carrying out specific practices, could be applied to promote carbon farming practices.In the present study, in addition to farmyard manure (FYM), cowpea was applied as green manure and faba bean as an intercrop in an organic greenhouse tomato crop, aiming to increase the levels of soil N. Three experiments (E1, E2, E3) were carried out, in which legumes were either noninoculated or inoculated with rhizobia alone or together with plant growth, promoting rhizobacteria. Inoculation of legumes with rhizobia considerably increased N2 fixation in E1 but had no impact on N2 fixation in E2 and E3. In E1, the application of cowpea decreased yield because it imposed a stronger nematode infection as the cowpea plants acted as a good host for Meloidogyne. However, in E2 and E3 the nematode infection was successfully controlled and the legumes significantly increased the tomato yield when inoculated in E2, irrespective of legume inoculation in E3. The total N concentration in the tomato plant tissues was significantly increased by legume application in E2 and E3, but not in E1. These results show that legumes applied as green manure can successfully complement N supply via FYM in organic greenhouse tomato, while legume inoculation with rhizobia can increase the amounts of nitrogen provided to the crop via green manure.

", "keywords": ["0106 biological sciences", "2. Zero hunger", "pgpr", "S", "organic", "Agriculture", "04 agricultural and veterinary sciences", "rhizobia", "01 natural sciences", "faba bean", "6. Clean water", "cowpea", "bnf", "Cowpea", "BNF", "PGPR", "0401 agriculture", " forestry", " and fisheries", "root-knot nematodes"]}, "links": [{"href": "http://www.mdpi.com/2073-4395/9/11/766/pdf"}, {"href": "https://iris.unito.it/bitstream/2318/1715802/1/Agronomy_9_766.pdf"}, {"href": "https://www.mdpi.com/2073-4395/9/11/766/pdf"}, {"href": "https://doi.org/10.3390/agronomy9110766"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/agronomy9110766", "name": "item", "description": "10.3390/agronomy9110766", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/agronomy9110766"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-17T00:00:00Z"}}, {"id": "10.3390/plants10112322", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:48Z", "type": "Journal Article", "created": "2021-10-29", "title": "Radial Oxygen Loss from Plant Roots\u2014Methods", "description": "

In flooded soils, an efficient internal aeration system is essential for root growth and plant survival. Roots of many wetland species form barriers to restrict radial O2 loss (ROL) to the rhizosphere. The formation of such barriers greatly enhances longitudinal O2 diffusion from basal parts towards the root tip, and the barrier also impedes the entry of phytotoxic compounds produced in flooded soils into the root. Nevertheless, ROL from roots is an important source of O2 for rhizosphere oxygenation and the oxidation of toxic compounds. In this paper, we review the methodological aspects for the most widely used techniques for the qualitative visualization and quantitative determination of ROL from roots. Detailed methodological approaches, practical set-ups and examples of ROL from roots with or without barriers to ROL are included. This paper provides practical knowledge relevant to several disciplines, including plant\u2013soil interactions, biogeochemistry and eco-physiological aspects of roots and soil biota.

", "keywords": ["0301 basic medicine", "0303 health sciences", "Botany", "microelectrodes", "root-sleeving electrodes", "planar optodes", "Review", "15. Life on land", "microsensors", "6. Clean water", "methylene blue staining", "03 medical and health sciences", "QK1-989", "Methylene blue staining; Microelectrodes; Microsensors; Planar optodes; Root-sleeving electrodes"]}, "links": [{"href": "https://air.uniud.it/bitstream/11390/1215619/1/plants-10-02322.pdf"}, {"href": "https://doi.org/10.3390/plants10112322"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plants", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/plants10112322", "name": "item", "description": "10.3390/plants10112322", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/plants10112322"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-10-28T00:00:00Z"}}, {"id": "10.5281/zenodo.10203450", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:22:45Z", "type": "Dataset", "title": "Quantification of soil organic carbon: the challenge of biochar-induced spatial heterogeneity", "description": "R-script and output from model on spatially discrete biochar application and its influence on representative SOC sampling. An additional document to explain the data curation is also available ('Comment on Data curation').", "keywords": ["modelling", "pyrogenic carbon capture and storage", "representative sampling", "biochar carbon removal", "15. Life on land", "root-zone application", "agroforestry"], "contacts": [{"organization": "ITHAKA", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.10203450"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.10203450", "name": "item", "description": "10.5281/zenodo.10203450", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.10203450"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-11-24T00:00:00Z"}}, {"id": "10.5281/zenodo.8089896", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:24:41Z", "type": "Journal Article", "created": "2020-10-09", "title": "Effects of water deficit and nitrogen application on leaf gas exchange, phytohormone signaling, biomass and water use efficiency of oat plants", "description": "Abstract

Background: Water and nitrogen (N) are essential resources influencing plant growth and yield. To improve their efficiencies in crop production is challenging because the physiological mechanisms of water and N coupling and their interactive effect on crop water use efficiency (WUE) are not well understood yet.

Aim: The aim of this study was to investigate the physiological responses and phytohormones signaling in oats in response to soil water status and N supply under fertigation, to explore the mechanisms regulating plant growth and WUE.

Methods: Oat plants were subjected to the factorial combination of three soil moisture regimes (50, 70, and 90% of soil water holding capacity, SWHC) and three N levels (fertilized with 74, 149, and 298 mg kg\uffe2\uff88\uff921).

Results: The stomatal conductance (gs) was significantly decreased by soil water deficit, and also by the highest N level, whereas photosynthesis rate (An) was unaffected by neither water nor N. Consequently, intrinsic WUE (WUEint, An/gs) was highest under reduced irrigation and high N fertilization. This effect at stomatal level was affirmed by responses in whole plant WUE (WUEb), which was positively correlated with shoot \uffce\uffb413C. A positive correlation between \uffce\uffb418O and \uffce\uffb413C in shoots further indicated that decreases of gs rather than changes in An contributed to the enhanced WUE.

Conclusion: Moderate soil water deficit and sufficient N supply is recommended for saving irrigation water and improving WUE on fertigated oat plants without compromising biomass accumulation to any large extent.

Soil compaction (SC) is a major threat for agriculture in Europe that affects many ecosystem functions, such as water and air circulation in soils, root growth, and crop production. Our objective was to present the results from five short-term (<5 years) case studies located along the north\u2013south and east\u2013west gradients and conducted within the SoilCare project using soil-improving cropping systems (SICSs) for mitigating topsoil and subsoil SC. Two study sites (SSs) focused on natural subsoil (\u02c325 cm) compaction using subsoiling tillage treatments to depths of 35 cm (Sweden) and 60 cm (Romania). The other SSs addressed both topsoil and subsoil SC (\u02c325 cm, Norway and United Kingdom; \u02c330 cm, Italy) using deep-rooted bio-drilling crops and different tillage types or a combination of both. Each SS evaluated the effectiveness of the SICSs by measuring the soil physical properties, and we calculated SC indices. The SICSs showed promising results\u2014for example, alfalfa in Norway showed good potential for alleviating SC (the subsoil density decreased from 1.69 to 1.45 g cm\u22121) and subsoiling at the Swedish SS improved root penetration into the subsoil by about 10 cm\u2014but the effects of SICSs on yields were generally small. These case studies also reflected difficulties in implementing SICSs, some of which are under development, and we discuss methodological issues for measuring their effectiveness. There is a need for refining these SICSs and for evaluating their longer-term effect under a wider range of pedoclimatic conditions.

", "keywords": ["bio-drilling crops", "ROOT-GROWTH", "Environmental Studies", "subsoiling", "PHYSICAL-PROPERTIES", "Soil Science", "Environmental Sciences & Ecology", "straw incorporation", "910", "CONSERVATION AGRICULTURE", "3301 Architecture", "soil penetration resistance", "4104 Environmental management", "degree of compaction; soil penetration resistance; relative normalised density; air-filled porosity; tillage; straw incorporation; bio-drilling crops; subsoiling; crop productivity", "relative normalised density", "GAS-TRANSPORT", "0502 Environmental Science and Management", "S Agriculture (General)", "910 Geography & travel", "PENETRATION RESISTANCE", "550 Earth sciences & geology", "crop productivity", "2. Zero hunger", "Science & Technology", "CLIMATE-CHANGE", "S", "degree of compaction", "3304 Urban and regional planning", "Agriculture", "04 agricultural and veterinary sciences", "DEEP-TILLAGE", "15. Life on land", "6. Clean water", "Environmental Sciences related to Agriculture and Land-use", "13. Climate action", "tillage", "0401 agriculture", " forestry", " and fisheries", "CLAY LOAM SOIL", "RISK-ASSESSMENT", "SUBSOIL COMPACTION", "Life Sciences & Biomedicine", "air-filled porosity"]}, "links": [{"href": "http://www.mdpi.com/2073-445X/11/2/223/pdf"}, {"href": "https://pub.epsilon.slu.se/27668/1/piccoli-i-et-al-220502.pdf"}, {"href": "https://boris.unibe.ch/165197/1/Opportunities_for_Mitigating_Soil_Compaction_in_Europe_Case.pdf"}, {"href": "https://www.research.unipd.it/bitstream/11577/3462067/1/land-11-00223-v2.pdf"}, {"href": "https://rau.repository.guildhe.ac.uk/id/eprint/16542/1/land-11-00223-v2.pdf"}, {"href": "https://doi.org/11250/3007222"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Land", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11250/3007222", "name": "item", "description": "11250/3007222", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11250/3007222"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-02T00:00:00Z"}}, {"id": "11390/1215619", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:26:00Z", "type": "Journal Article", "created": "2021-10-28", "title": "Radial Oxygen Loss from Plant Roots\u2014Methods", "description": "

In flooded soils, an efficient internal aeration system is essential for root growth and plant survival. Roots of many wetland species form barriers to restrict radial O2 loss (ROL) to the rhizosphere. The formation of such barriers greatly enhances longitudinal O2 diffusion from basal parts towards the root tip, and the barrier also impedes the entry of phytotoxic compounds produced in flooded soils into the root. Nevertheless, ROL from roots is an important source of O2 for rhizosphere oxygenation and the oxidation of toxic compounds. In this paper, we review the methodological aspects for the most widely used techniques for the qualitative visualization and quantitative determination of ROL from roots. Detailed methodological approaches, practical set-ups and examples of ROL from roots with or without barriers to ROL are included. This paper provides practical knowledge relevant to several disciplines, including plant\u2013soil interactions, biogeochemistry and eco-physiological aspects of roots and soil biota.

", "keywords": ["0301 basic medicine", "0303 health sciences", "Botany", "microelectrodes", "root-sleeving electrodes", "planar optodes", "Review", "15. Life on land", "microsensors", "6. Clean water", "methylene blue staining", "03 medical and health sciences", "QK1-989", "Methylene blue staining; Microelectrodes; Microsensors; Planar optodes; Root-sleeving electrodes"]}, "links": [{"href": "https://air.uniud.it/bitstream/11390/1215619/1/plants-10-02322.pdf"}, {"href": "https://doi.org/11390/1215619"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plants", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11390/1215619", "name": "item", "description": "11390/1215619", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11390/1215619"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-10-28T00:00:00Z"}}, {"id": "2801205749", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:26:56Z", "type": "Journal Article", "created": "2018-05-25", "title": "Correlative Visualization of Root Mucilage Degradation Using X-ray CT and MRI", "description": "Root exudates are a crucial component of the rhizosphere. Often, they take a form of a gel exuded by the plant roots and are thought to influence the soil aggregation, root penetration into soil, soil nutrient availability, immobilization of toxic cations, and microbial activity amongst other things. In addition, the capacity of exudates to store water makes the plants potentially less susceptive to drought. Major components of root exudates are high molecular weight organic compounds consisting of predominantly polysaccharides and proteins, which makes it challenging to visualize using current rhizosphere visualization techniques, such as X-ray computed tomography (CT). In this contribution, we use correlative X-ray CT (resolution ~20 \u03bcm) in combination with Magnetic Resonance Imaging (MRI, resolution ~120 \u03bcm) to set up groundwork to enable in situ visualization of mucilage in soil. This multimodal approach is necessary because mucilage density closely matches that of water. We use chia seeds as mucilage analog, because it has been found to have a similar consistency to root mucilage. Moreover, to understand mucilage development in time, a series of samples made by chia seeds placed in different porous media were prepared. Structurally and chemically, mucilage breaks down toward a water-like substance over a course of 2 weeks. Depending on its relative concentration, these changes were found to be less dominant when seeds were mixed in porous media. Having set up the groundwork for correlative imaging of chia seeds in water and an artificial soil (Nafion and sand/beads) this enables us to expand this imaging to deal with plant root exudates under natural conditions.", "keywords": ["2. Zero hunger", "570", "X-ray CT", "chia", "polysaccharides", "04 agricultural and veterinary sciences", "15. Life on land", "630", "6. Clean water", "root-exudate", "Environmental sciences", "1H-MRI", "0401 agriculture", " forestry", " and fisheries", "GE1-350", "mucilage"]}, "links": [{"href": "https://eprints.soton.ac.uk/421014/1/NMRI_imaging_chia_review_final_2_TR_01_05_2018.pdf"}, {"href": "https://eprints.soton.ac.uk/421014/2/fenvs_06_00032.pdf"}, {"href": "https://doi.org/2801205749"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2801205749", "name": "item", "description": "2801205749", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2801205749"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-25T00:00:00Z"}}, {"id": "2158/1304652", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:26:41Z", "type": "Journal Article", "created": "2022-02-14", "title": "Plant responses to heterogeneous salinity: agronomic relevance and research priorities", "description": "Abstract Background

Soil salinity, in both natural and managed environments, is highly heterogeneous, and understanding how plants respond to this spatiotemporal heterogeneity is increasingly important for sustainable agriculture in the era of global climate change. While the vast majority of research on crop response to salinity utilizes homogeneous saline conditions, a much smaller, but important, effort has been made in the past decade to understand plant molecular and physiological responses to heterogeneous salinity mainly by using split-root studies. These studies have begun to unravel how plants compensate for water/nutrient deprivation and limit salt stress by optimizing root-foraging in the most favourable parts of the soil.

Scope

This paper provides an overview of the patterns of salinity heterogeneity in rain-fed and irrigated systems. We then discuss results from split-root studies and the recent progress in understanding the physiological and molecular mechanisms regulating plant responses to heterogeneous root-zone salinity and nutrient conditions. We focus on mechanisms by which plants (salt/nutrient sensing, root-shoot signalling and water uptake) could optimize the use of less-saline patches within the root-zone, thereby enhancing growth under heterogeneous soil salinity conditions. Finally, we place these findings in the context of defining future research priorities, possible irrigation management and crop breeding opportunities to improve productivity from salt-affected lands.

", "keywords": ["Nutrient heterogeneity", "Water uptake", "Root-to-shoot signalling", "Salinity", "550", "Plant Biology & Botany", "Plant Biology", "Irrigation; nutrient heterogeneity; phytohormones; root foraging; root-to-shoot signalling; salt sensing; stomatal conductance; water uptake", "Stomatal conductance", "Salt sensing", "Plant Roots", "630", "12. Responsible consumption", "root foraging", "Soil", "Irrigation", "salt sensing", "Root foraging", "580", "2. Zero hunger", "Ecology", "Forestry Sciences", "Research", "nutrient heterogeneity", "Water", "15. Life on land", "6. Clean water", "root-to-shoot signalling", "phytohormones", "Phytohormones", "stomatal conductance", "13. Climate action", "Zero Hunger", "water uptake"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/166913/1/21783_2_merged_1643798007.pdf"}, {"href": "https://academic.oup.com/aob/article-pdf/129/5/499/43374309/mcac022.pdf"}, {"href": "https://escholarship.org/content/qt7t32v7cc/qt7t32v7cc.pdf"}, {"href": "https://doi.org/2158/1304652"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Annals%20of%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2158/1304652", "name": "item", "description": "2158/1304652", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2158/1304652"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-16T00:00:00Z"}}, {"id": "2440/137248", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:26:49Z", "type": "Journal Article", "created": "2022-08-26", "title": "Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress", "description": "Abstract

We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these questions include the need to better understand how plants detect water availability, temperature, salinity, and rising carbon dioxide (CO2) levels; how environmental signals interface with endogenous signaling and development (e.g. circadian clock and flowering time); and how this integrated signaling controls downstream responses (e.g. stomatal regulation, proline metabolism, and growth versus defense balance). The plasma membrane comes up frequently as a site of key signaling and transport events (e.g. mechanosensing and lipid-derived signaling, aquaporins). Adaptation to water extremes and rising CO2 affects hydraulic architecture and transpiration, as well as root and shoot growth and morphology, in ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution and crop resilience in the face of changing and increasingly unpredictable environments. Exploration of plant diversity within and among species can help us know which of these tradeoffs represent fundamental limits and which ones can be circumvented by bringing new trait combinations together. Better defining what constitutes beneficial stress resistance in different contexts and making connections between genes and phenotypes, and between laboratory and field observations, are overarching challenges.Background: Water and nitrogen (N) are essential resources influencing plant growth and yield. To improve their efficiencies in crop production is challenging because the physiological mechanisms of water and N coupling and their interactive effect on crop water use efficiency (WUE) are not well understood yet.

Aim: The aim of this study was to investigate the physiological responses and phytohormones signaling in oats in response to soil water status and N supply under fertigation, to explore the mechanisms regulating plant growth and WUE.

Methods: Oat plants were subjected to the factorial combination of three soil moisture regimes (50, 70, and 90% of soil water holding capacity, SWHC) and three N levels (fertilized with 74, 149, and 298 mg kg\uffe2\uff88\uff921).

Results: The stomatal conductance (gs) was significantly decreased by soil water deficit, and also by the highest N level, whereas photosynthesis rate (An) was unaffected by neither water nor N. Consequently, intrinsic WUE (WUEint, An/gs) was highest under reduced irrigation and high N fertilization. This effect at stomatal level was affirmed by responses in whole plant WUE (WUEb), which was positively correlated with shoot \uffce\uffb413C. A positive correlation between \uffce\uffb418O and \uffce\uffb413C in shoots further indicated that decreases of gs rather than changes in An contributed to the enhanced WUE.

Conclusion: Moderate soil water deficit and sufficient N supply is recommended for saving irrigation water and improving WUE on fertigated oat plants without compromising biomass accumulation to any large extent.Our understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.Our understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.