{"type": "FeatureCollection", "features": [{"id": "10.1111/j.1461-0248.2010.01570.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:18:39Z", "type": "Journal Article", "created": "2010-12-22", "title": "Enhanced Root Exudation Induces Microbial Feedbacks To N Cycling In A Pine Forest Under Long-Term Co2 Fumigation", "description": "

Ecology Letters (2011) 14: 187\uffe2\uff80\uff93194

Abstract

The degree to which rising atmospheric CO2 will be offset by carbon (C) sequestration in forests depends in part on the capacity of trees and soil microbes to make physiological adjustments that can alleviate resource limitation. Here, we show for the first time that mature trees exposed to CO2 enrichment increase the release of soluble C from roots to soil, and that such increases are coupled to the accelerated turnover of nitrogen (N) pools in the rhizosphere. Over the course of 3\uffe2\uff80\uff83years, we measured in situ rates of root exudation from 420 intact loblolly pine (Pinus taeda L.) roots. Trees fumigated with elevated CO2 (200 p.p.m.v. over background) increased exudation rates (\uffce\uffbcg\uffe2\uff80\uff83C\uffe2\uff80\uff83cm\uffe2\uff88\uff921\uffe2\uff80\uff83root\uffe2\uff80\uff83h\uffe2\uff88\uff921) by 55% during the primary growing season, leading to a 50% annual increase in dissolved organic inputs to fumigated forest soils. These increases in root\uffe2\uff80\uff90derived C were positively correlated with microbial release of extracellular enzymes involved in breakdown of organic N (R2\uffe2\uff80\uff83=\uffe2\uff80\uff830.66; P\uffe2\uff80\uff83=\uffe2\uff80\uff830.006) in the rhizosphere, indicating that exudation stimulated microbial activity and accelerated the rate of soil organic matter (SOM) turnover. In support of this conclusion, trees exposed to both elevated CO2 and N fertilization did not increase exudation rates and had reduced enzyme activities in the rhizosphere. Collectively, our results provide field\uffe2\uff80\uff90based empirical support suggesting that sustained growth responses of forests to elevated CO2 in low fertility soils are maintained by enhanced rates of microbial activity and N cycling fuelled by inputs of root\uffe2\uff80\uff90derived C. To the extent that increases in exudation also stimulate SOM decomposition, such changes may prevent soil C accumulation in forest ecosystems.

", "keywords": ["0106 biological sciences", "Nitrogen", "Plant Exudates", "Pinus taeda", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon Dioxide", "01 natural sciences", "Plant Roots", "Carbon", "Trees", "Soil", "13. Climate action", "Rhizosphere", "North Carolina", "0401 agriculture", " forestry", " and fisheries", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1111/j.1461-0248.2010.01570.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1461-0248.2010.01570.x", "name": "item", "description": "10.1111/j.1461-0248.2010.01570.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1461-0248.2010.01570.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-12-22T00:00:00Z"}}, {"id": "10.1007/s00442-012-2578-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:32Z", "type": "Journal Article", "created": "2013-01-07", "title": "Effects Of Drought And N-Fertilization On N Cycling In Two Grassland Soils", "description": "Open AccessOecologia, 171 (3)", "keywords": ["[SDE] Environmental Sciences", "N2O fluxes", "550", "functional genes", "Nitrogen", "[SDV]Life Sciences [q-bio]", "Climate", "Climate Change", "Nitrification and denitrification", "enzyme activites", "Urine", "630", "10127 Institute of Evolutionary Biology and Environmental Studies", "Soil", "Quantitative PCR", "Climate change; Enzyme activities; Functional genes; Quantitative PCR; Nitrification and denitrification; N2O fluxes", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Animals", "Climate change", "Enzyme activities", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "Ecosystem", "Soil Microbiology", "Functional genes", "Nitrogen Cycle", "Plants", "Archaea", "Droughts", "[SDV] Life Sciences [q-bio]", "1105 Ecology", " Evolution", " Behavior and Systematics", "climate change", "Genes", " Bacterial", "[SDE]Environmental Sciences", "quantitative PCR", "Denitrification", "570 Life sciences; biology", "590 Animals (Zoology)", "Cattle", "nitrification and denitrification"]}, "links": [{"href": "https://doi.org/10.1007/s00442-012-2578-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-012-2578-3", "name": "item", "description": "10.1007/s00442-012-2578-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-012-2578-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-01-08T00:00:00Z"}}, {"id": "10.1111/gcb.12338", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:18:25Z", "type": "Journal Article", "created": "2013-07-29", "title": "Investigating The Long-Term Legacy Of Drought And Warming On The Soil Microbial Community Across Five European Shrubland Ecosystems", "description": "Abstract

We investigated how the legacy of warming and summer drought affected microbial communities in five different replicated long\uffe2\uff80\uff90term (>10\uffc2\uffa0years) field experiments across Europe (EU\uffe2\uff80\uff90FP7 INCREASE infrastructure). To focus explicitly on legacy effects (i.e., indirect rather than direct effects of the environmental factors), we measured microbial variables under the same moisture and temperature in a brief screening, and following a pre\uffe2\uff80\uff90incubation at stable conditions. Specifically, we investigated the size and composition of the soil microbial community (PLFA) alongside measurements of bacterial (leucine incorporation) and fungal (acetate in ergosterol incorporation) growth rates, previously shown to be highly responsive to changes in environmental factors, and microbial respiration. We found no legacy effects on the microbial community size, composition, growth rates, or basal respiration rates at the effect sizes used in our experimental setup (0.6\uffc2\uffa0\uffc2\uffb0C, about 30% precipitation reduction). Our findings support previous reports from single short\uffe2\uff80\uff90term ecosystem studies thereby providing a clear evidence base to allow long\uffe2\uff80\uff90term, broad\uffe2\uff80\uff90scale generalizations to be made. The implication of our study is that warming and summer drought will not result in legacy effects on the microbial community and their processes within the effect sizes here studied. While legacy effects on microbial processes during perturbation cycles, such as drying\uffe2\uff80\uff93rewetting, and on tolerance to drought and warming remain to be studied, our results suggest that any effects on overall ecosystem processes will be rather limited. Thus, the legacies of warming and drought should not be prioritized factors to consider when modeling contemporary rates of biogeochemical processes in soil.

", "keywords": ["2. Zero hunger", "decomposition", "Hot Temperature", "Bacteria", "soil C cycle", "Climate Change", "global climate change", "warming adaptation", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "carbon sequestration", "6. Clean water", "ecosystem service", "Droughts", "Europe", "Leucine", "13. Climate action", "temperature acclimation", "0401 agriculture", " forestry", " and fisheries", "mineralization", "Seasons", "Ecosystem", "Soil Microbiology", "Acetic Acid"]}, "links": [{"href": "https://doi.org/10.1111/gcb.12338"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.12338", "name": "item", "description": "10.1111/gcb.12338", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.12338"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-10-10T00:00:00Z"}}, {"id": "10.1016/j.envint.2020.106190", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:16:04Z", "type": "Journal Article", "created": "2020-10-26", "title": "Treated wastewater irrigation promotes the spread of antibiotic resistance into subsoil pore-water", "description": "In the present study, we investigated the impact of treated wastewater (TWW) irrigation on the prevalence of antibiotic resistance genes (ARGs) in subsoil pore-water, a so-far under-appreciated matrix. We hypothesized that TWW irrigation increases ARG prevalence in subsoil pore-water. This hypothesis was tested using a multiphase approach, which consisted of sampling percolated subsoil pore-water from lysimeter-wells of a real-scale TWW-irrigated field, operated for commercial farming practices, and controlled, laboratory microcosms irrigated with freshwater or TWW. We monitored the abundance of six selected ARGs (sul1, blaOXA-58, tetM, qnrS, blaCTX-M-32 and blaTEM), the intI1 gene associated with mobile genetic elements and an indicator for anthropogenic pollution and bacterial abundance (16S rRNA gene) by qPCR. The bacterial load of subsoil pore water was independent of both, irrigation intensity in the field study and irrigation water type in the microcosms. Among the tested genes in the field study, sul1 and intI1 exhibited constantly higher relative abundances. Their abundance was further positively correlated with increasing irrigation intensity. Controlled microcosm experiments verified the observed field study results: the relative abundance of several genes, including sul1 and intI1, increased significantly when irrigating with TWW compared to freshwater irrigation. Overall, TWW irrigation promoted the spread of ARGs and intI1 in the subsoil pore-water, while the bacterial load was maintained. The combined results from the real-scale agricultural field and the controlled lab microcosms indicate that the dissemination of ARGs in various subsurface environments needs to be taken into account during TWW irrigation scenarios.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Agricultural Irrigation", "Antibiotic resistance", "Water", "Subsoil pore-water", "Drug Resistance", " Microbial", "Wastewater", "Wastewater reuse", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Environmental sciences", "qPCR", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "RNA", " Ribosomal", " 16S", "ARGs", "GE1-350", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envint.2020.106190"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environment%20International", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envint.2020.106190", "name": "item", "description": "10.1016/j.envint.2020.106190", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envint.2020.106190"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "oai:opus.uni-hohenheim.de:2046", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:31:39Z", "type": "Report", "title": "Soil microorganisms as hidden miners of phosphorus in soils under different cover crop and tillage treatments", "description": "Phosphorus (P) is one of the most limiting plant nutrients for agricultural production. The soil microbial community plays a key role in nutrient cycling, affecting access of roots to P, as well as mobilization and mineralization of organic P (Porg). This thesis aimed to better understand the potential of cover crops to enhance plant-soil-microbe interactions to improve the availability of P. This dissertation consists of a meta-analysis of and two field experiments. The used methods showed that microbial P, the activity of P-cycling enzymes and PLFAs increased under cover crops, indicating an enhanced potential for organic P cycling. Gram- positive and Gram-negative bacteria, and to a lesser extent also arbuscular mycorrhizal fungi, increased their abundance with cover crops. However, saprotrophic fungi could benefit most from the substrate input derived from cover crop roots or litter. Enzyme-stable Porg shifted towards pools of a greater lability in the active soil compartments (rhizosheath and detritusphere). The effects of agricultural management, such as cover crop species choice and tillage, were detectable, but weaker compared to the effect of the presence of cover crops. With the obtained results, the research aims of this thesis could be successfully addressed. We were able to confirm that cover crops have the potential to improve main crops\u0092 access to P. Furthermore, we presented and discussed three pathways of P benefit. In the plant biomass pathway, P is cycled through cover crop biomass and becomes available for the main crop upon litter decomposition. The microbial enhancement pathway describes how the cover crop\u0092s interaction with soil microbes increases their abundance and activity, thereby increasing the availability of Porg. Some cover crop species seem to be capable of utilizing a biochemical modification pathway, where changes in the sorption capacity of the soil result in a greater quantity of plant-available phosphate. However, the latter pathway was apparently not important in the crop rotations used in our field experiments. The data also allowed us to characterize ways in which plant-soil-microbe interactions under cover crops affected the relationship of soil microbial functions to the enzymatic availability of Porg pools. Cover crops increased the abundance and activity of microbes, especially fungi, as well as microbial P. This enhancement in P-cycling potential shifted Porg toward pools of greater availability to added enzymes. However, the relation between enzymes and Porg pools is complex and is possibly affected by soil P composition and other site characteristics, indicating the need for further research in this area. Finally, we elucidated how the choice of cover crop species and agricultural management can shift the relative importance of the pathways for the P benefit of the main crop, while site-specific management allows farmers to adapt to local conditions and to optimize the functions of their agroecosystems. In conclusion, our results indicate that the pathways of cover crop derived P benefit take place simultaneously. We confirmed the potential of cover crop biomass for the cycling of P, and we suggest that our observed increases in the availability of soil Porg are related to microbial abundance and activity. The interactions of cover cropping and tillage indicate also that P benefit can be optimized by management decisions. Finally, these new insights into soil phosphorus cycling in agroecosystems have the potential to support further development of more sustainable agricultural systems. Phosphor (P) ist einer der wichtigsten limitierenden Naehrstoffe fuer das Pflanzenwachstum in der Landwirtschaft. Bodenmikroben spielen eine Schluesselrolle in Naehrstoffkreislaeufen, beeinflussen das Wachstum von Pflanzenwurzeln, die Mobilisierung sowie die Mineralisierung von organischem P (Porg) und somit den Zugang zu P. Das Ziel dieser Dissertation war die Einschaetzung des Potentials von Zwischenfruechten zur Verbesserung der Interaktionen im System Pflanze-Boden-Mikroben und einer dadurch moeglichen Steigerung der P-Verfuegbarkeit fuer die Hauptfruechte. Diese Dissertation umfasst drei wissenschaftliche Veroeffentlichungen: Eine Literaturrecherche und Meta-Analyse sowie zwei selbst durchgefuehrte Feldexperimente. Die verwendeten Methoden zeigten, dass Zwischenfruechte den P-Gehalt in der mikrobiellen Biomasse, die Aktivitaet von Phosphatasen und mikrobielle Fettsaeuremarker (PLFAs) erhoehen, was auf ein gesteigertes Umsatzpotential von organischen Phosphorverbindungen hindeutet. Die Abundanz von grampositiven und gramnegativen Bakterien, sowie in geringerem Umfang auch von arbuskulaeren Mykorrhizapilzen, wurde durch Zwischenfruechte erhoeht. Gleichwohl waren saprotrophe Bodenpilze die mikrobielle Gruppe, die am meisten von der Substratzufuhr der Wurzeln und Streu profitieren konnte. Stabiles P wurde in den aktiven Bodenzonen der Rhizosphaere und Detritusphaere in labilere Porg-Pools transformiert. Bewirtschaftungseffekte, wie die Wahl der Zwischenfrucht oder Bodenbearbeitung, waren erkennbar, aber wesentlich schwaecher ausgepraegt als der Zwischenfruchteffekt insgesamt. Unsere Ergebnisse bestaetigen, dass Zwischenfruchtanbau zur Steigerung der P - Verfuegbarkeit fuer die Hauptfrucht fuehren kann. Darueber hinaus konnten wir fuer den P- Vorteil drei grundsaetzliche Wirkungspfade aufzeigen, die in aktiven Bodenraeumen stattfinden. UEber den Wirkungspfad \u0084Pflanzenbiomasse\u0093 wird P aus dem Boden in die Biomasse der Zwischenfrucht aufgenommen und waehrend der Zersetzung der Streu fuer die Hauptfrucht verfuegbar. UEber den Wirkungspfad \u0084mikrobielle Verstaerkung\u0093 steigert die Zwischenfrucht im Wurzelraum die Biomasse und Aktivitaet der mikrobiellen Gemeinschaft, wodurch diese die Verfuegbarkeit von Porg erhoehen kann. Durch den Wirkungspfad \u0084biochemische Modifikation\u0093 scheinen manche Zwischenfruchtarten in der Lage zu sein, ueber Wurzelexsudate die P-Sorption im Boden zu senken und dadurch den Anteil an pflanzenverfuegbarem Phosphat zu erhoehen. Weiterhin ermoeglichen die erhobenen Daten die Diskussion, inwiefern mikrobielle Funktionen und die Mineralisierbarkeit von Porg zusammenhaengen und wie die Interaktionen von Pflanzen beeinflusst werden. Zwischenfruechte steigerten sowohl die Abundanz und Aktivitaet von Mikroben, als auch die Menge an P in der mikrobiellen Biomasse. Diese Potentialsteigerung des P-Kreislaufs steigerte die Verfuegbarkeit des Porg fuer zugefuegte Enzyme. Es muss bedacht werden, dass die Rueckkopplungen zwischen Enzymaktivitaet und verschiedenen Porg-Pools komplex sind. Diese haengen von den lokalen Eigenschaften des Bodens, wie etwa der Zusammensetzung des P-Vorrats, ab und sollten durch zukuenftige Studien geklaert werden. Drittens zeigen unsere Untersuchungen, wie die Wahl der Zwischenfrucht und die der Bewirtschaftung (z.B. Bodenbearbeitung oder Fruchtfolge) die relative Gewichtung der verschiedenen Pfade des P-Vorteils fuer die Hauptfrucht beeinflussen. Standortangepasste Zwischenfruchtsysteme erlauben es Landwirt:innen, die Funktionen ihres Agrooekosystems hinsichtlich der lokalen Bedingungen zu optimieren. Zusammenfassend bestaetigen unsere Ergebnisse, dass der P-Bedarf der Hauptfrucht ueber die Biomasse der Zwischenfrucht gedeckt werden kann und zeigen auf, dass die charakterisierten drei Pfade des P-Vorteils durch Zwischenfruchtanbau parallel stattfinden. Schlie\u00dflich koennen die hier gewonnenen Erkenntnisse ueber den Phosphorkreislauf, basierend auf der Kombination von bodenmikrobiologischen Methoden mit der Charakterisierung der Labilitaet von Porg, zur zukuenftigen Entwicklung einer nachhaltigeren Landwirtschaft beitragen.", "keywords": ["830", "Bodenmikrobiologie", "Fruchtfolge", "phosphorus mobilization", "Agriculture", "Phosphor", "N\u00e4hrstoffkreislauf", "Zwischenfrucht", "630", "soil microbiology", "Bodenmikroorganismus", "sustainable agriculture", "nutrient management", "ddc:630", "Phosphorkreislauf"], "contacts": [{"organization": "Hallama, Moritz", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/oai:opus.uni-hohenheim.de:2046"}, {"rel": "self", "type": "application/geo+json", "title": "oai:opus.uni-hohenheim.de:2046", "name": "item", "description": "oai:opus.uni-hohenheim.de:2046", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/oai:opus.uni-hohenheim.de:2046"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "PMC5507504", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:27:08Z", "type": "Journal Article", "created": "2017-07-13", "title": "Organic farming enhances soil microbial abundance and activity\u2014A meta-analysis and meta-regression", "description": "Population growth and climate change challenge our food and farming systems and provide arguments for an increased intensification of agriculture. A promising option is eco-functional intensification through organic farming, an approach based on using and enhancing internal natural resources and processes to secure and improve agricultural productivity, while minimizing negative environmental impacts. In this concept an active soil microbiota plays an important role for various soil based ecosystem services such as nutrient cycling, erosion control and pest and disease regulation. Several studies have reported a positive effect of organic farming on soil health and quality including microbial community traits. However, so far no systematic quantification of whether organic farming systems comprise larger and more active soil microbial communities compared to conventional farming systems was performed on a global scale. Therefore, we conducted a meta-analysis on current literature to quantify possible differences in key indicators for soil microbial abundance and activity in organic and conventional cropping systems. All together we integrated data from 56 mainly peer-reviewed papers into our analysis, including 149 pairwise comparisons originating from different climatic zones and experimental duration ranging from 3 to more than 100 years. Overall, we found that organic systems had 32% to 84% greater microbial biomass carbon, microbial biomass nitrogen, total phospholipid fatty-acids, and dehydrogenase, urease and protease activities than conventional systems. Exclusively the metabolic quotient as an indicator for stresses on microbial communities remained unaffected by the farming systems. Categorical subgroup analysis revealed that crop rotation, the inclusion of legumes in the crop rotation and organic inputs are important farming practices affecting soil microbial community size and activity. Furthermore, we show that differences in microbial size and activity between organic and conventional farming systems vary as a function of land use (arable, orchards, and grassland), plant life cycle (annual and perennial) and climatic zone. In summary, this study shows that overall organic farming enhances total microbial abundance and activity in agricultural soils on a global scale.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Organic Agriculture", "Science", "Climate", "Microbiota", "Q", "R", "Fabaceae", "04 agricultural and veterinary sciences", "15. Life on land", "13. Climate action", "Life Science", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/PMC5507504"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC5507504", "name": "item", "description": "PMC5507504", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC5507504"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-12T00:00:00Z"}}, {"id": "10.1002/ecy.1513", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:03Z", "type": "Journal Article", "created": "2016-07-02", "title": "Land Use Intensification In The Humid Tropics Increased Both Alpha And Beta Diversity Of Soil Bacteria", "description": "Abstract

Anthropogenic pressures on tropical forests are rapidly intensifying, but our understanding of their implications for biological diversity is still very limited, especially with regard to soil biota, and in particular soil bacterial communities. Here we evaluated bacterial community composition and diversity across a gradient of land use intensity in the eastern Amazon from undisturbed primary forest, through primary forests varyingly disturbed by fire, regenerating secondary forest, pasture, and mechanized agriculture. Soil bacteria were assessed by paired\uffe2\uff80\uff90end Illumina sequencing of 16S rRNA gene fragments (V4 region). The resulting sequences were clustered into operational taxonomic units (OTU) at a 97% similarity threshold. Land use intensification increased the observed bacterial diversity (both OTU richness and community heterogeneity across space) and this effect was strongly associated with changes in soil pH. Moreover, land use intensification and subsequent changes in soil fertility, especially pH, altered the bacterial community composition, with pastures and areas of mechanized agriculture displaying the most contrasting communities in relation to undisturbed primary forest. Together, these results indicate that tropical forest conversion impacts soil bacteria not through loss of diversity, as previously thought, but mainly by imposing marked shifts on bacterial community composition, with unknown yet potentially important implications for ecological functions and services performed by these communities.

", "keywords": ["Rios de composi\u00e7\u00e3o de comunidade bacteriana", "2. Zero hunger", "0301 basic medicine", "570", "0303 health sciences", "550", "Bacteria", "Biodiversidade subterr\u00e2nea", "Agriculture", "Biodiversity", "Forests", "15. Life on land", "Below\u2010ground biodiversity", "High\u2010throughput sequencing", "Soil", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "Sequenciamento de alto rendimento", "Rivers of bacterial community composition", "Soil Microbiology"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/82660/1/de_Carvalho_et_al_2016_raw_pdf.pdf"}, {"href": "https://doi.org/10.1002/ecy.1513"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecy.1513", "name": "item", "description": "10.1002/ecy.1513", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecy.1513"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-09T00:00:00Z"}}, {"id": "10.1002/ecy.2137", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:03Z", "type": "Journal Article", "created": "2018-01-10", "title": "Ecological drivers of soil microbial diversity and soil biological networks in the Southern Hemisphere", "description": "Abstract

The ecological drivers of soil biodiversity in the Southern Hemisphere remain underexplored. Here, in a continental survey comprising 647 sites, across 58 degrees of latitude between tropical Australia and Antarctica, we evaluated the major ecological patterns in soil biodiversity and relative abundance of ecological clusters within a co\uffe2\uff80\uff90occurrence network of soil bacteria, archaea and eukaryotes. Six major ecological clusters (modules) of co\uffe2\uff80\uff90occurring soil taxa were identified. These clusters exhibited strong shifts in their relative abundances with increasing distance from the equator. Temperature was the major environmental driver of the relative abundance of ecological clusters when Australia and Antarctica are analyzed together. Temperature, aridity, soil properties and vegetation types were the major drivers of the relative abundance of different ecological clusters within Australia. Our data supports significant reductions in the diversity of bacteria, archaea and eukaryotes in Antarctica vs. Australia linked to strong reductions in temperature. However, we only detected small latitudinal variations in soil biodiversity within Australia. Different environmental drivers regulate the diversity of soil archaea (temperature and soil carbon), bacteria (aridity, vegetation attributes and pH) and eukaryotes (vegetation type and soil carbon) across Australia. Together, our findings provide new insights into the mechanisms driving soil biodiversity in the Southern Hemisphere.Recent studies have shown that invasive earthworms can dramatically reduce native biodiversity, both above and below the ground. However, we still lack a synthetic understanding of the underlying mechanisms behind these changes, such as whether earthworm effects on soil chemical properties drive such relationships. Here, we investigated the effects of invasive earthworms on soil chemical properties (pH, water content, and the stocks and fluxes of carbon, nitrogen, and phosphorus) by conducting a meta\uffe2\uff80\uff90analysis. Invasive earthworms generally increased soil pH, indicating that the removal of organic layers and the upward transport of more base\uffe2\uff80\uff90rich mineral soil caused a shift in soil pH. Moreover, earthworms significantly decreased soil water content, suggesting that the burrowing activities of earthworms may have increased water infiltration of and/or increased evapotranspiration from soil. Notably, invasive earthworms had opposing effects on organic and mineral soil for carbon and nitrogen stocks, with decreases in organic, and increases in mineral soil. Nitrogen fluxes were higher in mineral soil, whereas fluxes in organic soil were not significantly affected by the presence of invasive earthworms, indicating that earthworms mobilize and redistribute nutrients among soil layers and increase overall nitrogen loss from the soil. Invasive earthworm effects on element stocks increased with ecological group richness only in organic soil. Earthworms further decreased ammonium stocks with negligible effects on nitrate stocks in organic soil, whereas they increased nitrate stocks but not ammonium stocks in mineral soil. Notably, all of these results were consistent across forest and grassland ecosystems underlining the generality of our findings. However, we found some significant differences between studies that were conducted in the field (observational and experimental settings) and in the lab, such as that the effects on soil pH decreased from field to lab settings, calling for a careful interpretation of lab findings. Our meta\uffe2\uff80\uff90analysis provides strong empirical evidence that earthworm invasion may lead to substantial changes in soil chemical properties and element cycling in soil. Furthermore, our results can help explain the dramatic effects of invasive earthworms on native biodiversity, for example, shifts towards the dominance of grass species over herbaceous ones, as shown by recent meta\uffe2\uff80\uff90analyses.

", "keywords": ["Element flux", "Nitrogen", "Earthworm ecological group", "Forests", "Nitrate", "exotic earthworms", "Nutrient cycling", "nitrogen", "Article", "earthworm ecological group", "Soil", "nitrate", "Animals", "phosphorus", "soil carbon", "Oligochaeta", "Ecosystem", "Soil Microbiology", "water content", "Exotic earthworms", "2. Zero hunger", "Water content", "Plan_S-Compliant-TA", "pH", "nutrient cycling", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Nitrification", "Soil carbon", "nitrification", "ammonium", "13. Climate action", "international", "0401 agriculture", " forestry", " and fisheries", "element flux", "Ammonium"]}, "links": [{"href": "https://esajournals.onlinelibrary.wiley.com/doi/pdf/10.1002/ecy.2936"}, {"href": "https://doi.org/10.1002/ecy.2936"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecy.2936", "name": "item", "description": "10.1002/ecy.2936", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecy.2936"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-08T00:00:00Z"}}, {"id": "10.1002/etc.5400", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:04Z", "type": "Journal Article", "created": "2022-06-09", "title": "Arbuscular Mycorrhizal Fungi and the Need for a Meaningful Regulatory Plant Protection Product Testing Strategy", "description": "Abstract

Arbuscular mycorrhizal fungi (AMF) perform key soil ecosystem services and, because of their symbiotic relationship with plant roots, may be exposed to the plant protection products (PPPs) applied to soils and crops. In 2017, the European Food Safety Authority (EFSA) released a scientific opinion addressing the state of the science on risk assessment of PPPs for in-soil organisms, recommending the inclusion of AMF ecotoxicological testing in the PPP regulatory process. However, it is not clear how this can be implemented in a tiered, robust, and ecologically relevant manner. Through a critical review of current literature, we examine the recommendations made within the EFSA report and the methodologies available to integrate AMF into the PPP risk assessment and provide perspective and commentary on their agronomic and ecological relevance. We conclude that considerable research questions remain to be addressed prior to the inclusion of AMF into the in-soil organism risk assessment, many of which stem from the unique challenges associated with including an obligate symbiont within the PPP risk assessment. Finally, we highlight critical knowledge gaps and the further research required to enable development of relevant, reliable, and robust scientific tests alongside pragmatic and scientifically sound guidance to ensure that any future risk-assessment paradigm is adequately protective of the ecosystem services it aims to preserve. Environ Toxicol Chem 2022;41:1808\uffe2\uff80\uff931823. \uffc2\uffa9 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.BACKGROUND: Denitrification is a microbial process that has received considerable attention during the past decade since it can result in losses of added nitrogen fertilisers from agricultural soils. Paddy soil has been known to have strong denitrifying activity, but the denitrifying microorganisms responsible for fertilisers in paddy soil are not well known. The objective of this study was to explore the impacts of 17\uffe2\uff80\uff90year application of inorganic and organic fertiliser (rice straw) on the abundance and composition of a nosZ\uffe2\uff80\uff90denitrifier community in paddy soil. Soil samples were collected from CK plots (no fertiliser), N (nitrogen fertiliser), NPK (nitrogen, phosphorus and potassium fertilisers) and NPK + OM (NPK plus organic matter). The nitrous oxide reductase gene (nosZ) community composition was analysed using terminal restriction fragment length polymorphism, and the abundance was determined by quantitative PCR.

RESULTS: Both the largest abundance of nosZ\uffe2\uff80\uff90denitrifier and the highest potential denitrifying activity (PDA) occurred in the NPK + OM treatment with about four times higher than that in the CK and two times higher than that in the N and NPK treatments (no significant difference). Denitrifying community composition differed significantly among fertilisation treatments except for the comparison between CK and N treatments. Of the measured abiotic factors, total organic carbon was significantly correlated with the observed differences in community composition and abundance (P < 0.01 by Monte Carlo permutation).

CONCLUSION: This study shows that the addition of different fertilisers affects the size and composition of the nosZ\uffe2\uff80\uff90denitrifier community in paddy soil. Copyright \uffc2\uffa9 2011 Society of Chemical Industry

", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Bacteria", "Nitrogen", "0402 animal and dairy science", "Agriculture", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Carbon", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "Denitrification", "0405 other agricultural sciences", "Fertilizers", "Oxidoreductases", "Monte Carlo Method", "Polymorphism", " Restriction Fragment Length", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.4533"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.4533", "name": "item", "description": "10.1002/jsfa.4533", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.4533"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-07-27T00:00:00Z"}}, {"id": "10.1002/jsfa.4645", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:07Z", "type": "Journal Article", "created": "2011-10-12", "title": "Restricted Mineralization Of Fresh Organic Materials Incorporated Into A Subtropical Paddy Soil", "description": "Abstract

BACKGROUND: Microbial activities involved in the dynamics of organic matter determine the potential for organic carbon (C) accumulation in soil. To understand this for paddy soil, an incubation experiment (25 \uffc2\uffb0C, 45% water\uffe2\uff80\uff90holding capacity) was established using 14C\uffe2\uff80\uff90labelled glucose and rice straw (500 \uffc2\uffb5g C g\uffe2\uff88\uff921 soil) as substrates; an adjacent upland soil was used for comparison.

RESULTS: The amount of microbial biomass in the paddy soil was approximately 6 times larger and its turnover rate was 1.5\uffe2\uff80\uff933 times faster than in the upland soil. These proportions of 14C\uffe2\uff80\uff90labelled glucose and rice straw mineralized in the paddy soil were about 3% smaller (P < 0.01) than those in the upland soil. Also, there was no significant priming effect of fresh substrate additions on the mineralization of native organic C in the paddy soil, while the priming effect was significant in the upland soil.

CONCLUSION: Although the paddy soil contains a large amount of microbial biomass, which is also very active, the mineralization of fresh substrates is significantly restricted in this soil, along with a small priming effect. This favours the accumulation of organic C in paddy soils. Copyright \uffc2\uffa9 2011 Society of Chemical Industry

", "keywords": ["2. Zero hunger", "Minerals", "Tropical Climate", "Agriculture", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "Carbon Cycle", "Soil", "Glucose", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Ecosystem", "Soil Microbiology"], "contacts": [{"organization": "Ping Zhou, John Keith Syers, Hongzhao Yuan, Yirong Su, Ling Li, Jinshui Wu,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1002/jsfa.4645"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.4645", "name": "item", "description": "10.1002/jsfa.4645", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.4645"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-10-12T00:00:00Z"}}, {"id": "10.1002/jsfa.4647", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:07Z", "type": "Journal Article", "created": "2011-09-27", "title": "Response Of Soil Organic Carbon Mineralization In Typical Karst Soils Following The Addition Of 14c-Labeled Rice Straw And Caco3", "description": "Abstract

BACKGROUND: Organic substrates and calcium are important factors controlling organic matter turnover in Karst soils. To understand their effects on soil organic carbon (SOC) mineralization, an incubation experiment was conducted involving a control treatment (CK), the addition of a 14C\uffe2\uff80\uff90labeled rice straw (T1), CaCO3 (T2), and both 14C\uffe2\uff80\uff90labeled rice straw and CaCO3 (T3) to two types of Karst soils (terra fusca and rendzina) and a red soil from southwestern China.

RESULTS: Cumulative mineralization of the rice straw over 100 days in rendzina (22.96 mg kg\uffe2\uff88\uff921) and terra fusca (23.19 mg kg\uffe2\uff88\uff921) was higher than in the red soil (15.48 mg kg\uffe2\uff88\uff921; P < 0.05). Cumulative mineralization of native SOC decreased following addition of 14C\uffe2\uff80\uff90labeled rice straw in the rendzina and terra fusca but increased in the red soil (negative and positive priming effects on native SOC). The turnover times of 14C\uffe2\uff80\uff90labeled microbial biomass C (MBC) in the red soil, terra fusca and rendzina were 71 \uffc2\uffb1 2, 243 \uffc2\uffb1 20 and 254 \uffc2\uffb1 45 days, respectively. By adding CaCO3, the accumulation of SOC was greater in the Karst soils than in the red soil.

CONCLUSION: Although the interactions between rice straw decomposition and priming effects on native SOC are not yet understood, there was considerable variation between Karst and red soils. Soil calcium was a positive factor in maintaining SOC stability. MBC from rice straws was stable in terra fusca and rendzina, whereas it was active in the red soil. The Karst soils (terra fusca and rendzina) used in this study benefited SOC accumulation. Copyright \uffc2\uffa9 2011 Society of Chemical Industry

", "keywords": ["2. Zero hunger", "Carbon Isotopes", "Soil", "0401 agriculture", " forestry", " and fisheries", "Calcium", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "Soil Microbiology", "6. Clean water", "Calcium Carbonate", "Carbon Cycle"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.4647"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.4647", "name": "item", "description": "10.1002/jsfa.4647", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.4647"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-09-23T00:00:00Z"}}, {"id": "10.1002/jsfa.5550", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:07Z", "type": "Journal Article", "created": "2012-01-06", "title": "The Effect Of Rice Straw Incorporation Into Paddy Soil On Carbon Sequestration And Emissions In The Double Cropping Rice System", "description": "Abstract

BACKGROUND: Soil organic carbon (SOC) sequestration, methane emission, and the net carbon sink represented by rice straw incorporated into soil (RIS) were studied using long\uffe2\uff80\uff90term experimentation with rice straw incorporated into soil (LRIS) and short\uffe2\uff80\uff90term experimentation with different patterns of rice straw incorporated into soil (SPRIS).

RESULTS: Soil organic carbon could be improved by RIS combined with soil ploughing. The increased rate of SOC deposition per cultivated layer was 0.10 t C ha\uffe2\uff88\uff921 for 2.625 t ha\uffe2\uff88\uff921 straw incorporated each season in LRIS and 0.36 t C ha\uffe2\uff88\uff921 for 4.5 t straw ha\uffe2\uff88\uff921 season\uffe2\uff88\uff921 incorporated in SPRIS; the apparent SOC conversion by rice straw (stubble) was reduced as the amount of incorporated straw increased. However, RIS methane emission from paddy fields also significantly exacerbated the CH4 emission flux observed during the early and late rice growing seasons, which was increased by 75.0% (P < 0.01) and 251.5% (P < 0.01), respectively, compared with combined application of nitrogen, phosphorus and potassium fertiliser (NPK). The apparent methane conversion of straw was almost uniform with a similar rice yield and soil cultivating mode. Among the patterns of RIS, methane emission was significantly reduced under straw covering untilled land, and this property led to the lowest apparent methane conversion.

CONCLUSION: RIS with ploughing and tilling resulted in negative carbon sequestration because of increased methane emissions. A combined NPK application with only rice stubble incorporation may be sustainable for a higher rice yield, but this approach has a reduced rate of negative carbon sequestration in the paddy field. Straw covering with no tillage was the best measure to realise high yield and low carbon emission for RIS. Copyright \uffc2\uffa9 2012 Society of Chemical Industry

", "keywords": ["2. Zero hunger", "Agriculture", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "6. Clean water", "Soil", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Methane", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.5550"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.5550", "name": "item", "description": "10.1002/jsfa.5550", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.5550"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-01-06T00:00:00Z"}}, {"id": "10.1002/jsfa.6206", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:08Z", "type": "Journal Article", "created": "2013-05-01", "title": "Effect Of Organic, Conventional And Mixed Cultivation Practices On Soil Microbial Community Structure And Nematode Abundance In A Cultivated Onion Crop", "description": "AbstractBACKGROUND

Responses of the soil microbial and nematode community to organic and conventional agricultural practices were studied using the Teagasc Kinsealy Systems Comparison trial as the experimental system. The trial is a long\uffe2\uff80\uff90term field experiment which divides conventional and organic agriculture into component pest\uffe2\uff80\uff90control and soil treatment practices. We hypothesised that management practices would affect soil ecology and used community level physiological profiles, microbial and nematode counts, and denaturing gradient gel electrophoresis (DGGE) to characterise soil microbial communities in plots used for onion (Allium cepa L.) cultivation.

RESULTS

Microbial activity and culturable bacterial counts were significantly higher under fully organic management. Culturable fungi, actinomycete and nematode counts showed a consistent trend towards higher numbers under fully organic management but these data were not statistically significant. No differences were found in the fungal/bacterial ratio. DGGE banding patterns and sequencing of excised bands showed clear differences between treatments. Putative onion fungal pathogens were predominantly sequenced under conventional soil treatment practices whilst putative soil suppressive bacterial species were predominantly sequenced from the organic pest\uffe2\uff80\uff90control treatment plots.

CONCLUSION

Organic management increased microbial activity and diversity. Sequence data was indicative of differences in functional groups and warrants further investigation. \uffc2\uffa9 2013 Society of Chemical Industry

", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "Microbial diversity", "Nematoda", "Soil", "03 medical and health sciences", "Onions", "Animals", "DNA", " Fungal", "Ecosystem", "Soil Microbiology", "2. Zero hunger", "Organic Agriculture", "0303 health sciences", "Bacteria", "Denaturing Gradient Gel Electrophoresis", "0402 animal and dairy science", "Fungi", "Denaturing gradient gel electrophoresis", "Agriculture", "Biolog Eco-plates", "04 agricultural and veterinary sciences", "15. Life on land", "Community level physiological profile", "Organic agriculture", "0405 other agricultural sciences"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.6206"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.6206", "name": "item", "description": "10.1002/jsfa.6206", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.6206"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-06-07T00:00:00Z"}}, {"id": "10.1002/jsfa.8606", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:08Z", "type": "Journal Article", "created": "2017-07-31", "title": "How Are Arbuscular Mycorrhizal Associations Related To Maize Growth Performance During Short-Term Cover Crop Rotation?", "description": "AbstractBACKGROUND

Better cover crop management options aiming to maximize the benefits of arbuscular mycorrhizal fungi (AMF) to subsequent crops are largely unknown. We investigated the impact of cover crop management methods on maize growth performance and assemblages of AMF colonizing maize roots in a field trial. The cover crop treatments comprised Italian ryegrass, wheat, brown mustard and fallow in rotation with maize.

RESULTS

The diversity of AMF communities among cover crops used for maize management was significantly influenced by the cover crop and time course. Cover crops did not affect grain yield and aboveground biomass of subsequent maize but affected early growth. A structural equation model indicated that the root colonization, AMF diversity and maize phosphorus uptake had direct strong positive effects on yield performance.

CONCLUSION

AMF variables and maize performance were related directly or indirectly to maize grain yield, whereas root colonization had a positive effect on maize performance. AMF may be an essential factor that determines the success of cover crop rotational systems. Encouraging AMF associations can potentially benefit cover cropping systems. Therefore, it is imperative to consider AMF associations and crop phenology when making management decisions. \uffc2\uffa9 2017 Society of Chemical Industry

", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "0402 animal and dairy science", "Agriculture", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "Zea mays", "Crop Production", "03 medical and health sciences", "Mycorrhizae", "Lolium", "0405 other agricultural sciences", "Soil Microbiology", "Triticum", "Mustard Plant"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.8606"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.8606", "name": "item", "description": "10.1002/jsfa.8606", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.8606"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-09-11T00:00:00Z"}}, {"id": "10.1002/ps.3380", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:10Z", "type": "Journal Article", "created": "2012-06-20", "title": "Reproduction Allocation And Potential Mechanism Of Individual Allelopathic Rice Plants In The Presence Of Competing Barnyardgrass", "description": "Abstract

BACKGROUND: In spite of increasing knowledge of allelopathic rice as an efficient component involved in paddy weed management, relatively little is known about its reproduction in response to competing weeds. Reproduction allocation of individual allelopathic rice plants in relation to monoculture and mixed culture with competing barnyardgrass in a paddy field was studied, along with analyses of soil nutrients and microbial communities to understand the potential mechanism.

RESULTS: At a 1:1 barnyardgrass and rice mixture proportion identified from a replacement series study, biomass, grain yield and major parameters of individual allelopathic rice plants at the mature stage were increased by competing barnyardgrass. There was no difference in allelopathic rice root\uffe2\uff80\uff90zone soil ammonium N and Olsen P between monoculture and mixed culture. However, mixed culture altered soil microbial biomass C and communities. When mixed with barnyardgrass, allelopathic rice root zone had an 87% increase in soil microbial biomass C. Phospholipid fatty acid (PLFA) profiling indicated that the signature lipid biomarkers of bacteria, actinobacteria and fungi were affected by mixed culture. Principal component analysis clearly identified differences in the composition of PLFA in different soil samples.

CONCLUSION: Allelopathic rice specific changes in soil microbial communities may generate a positive feedback on its own growth and reproduction in the presence of competing barnyardgrass in a given paddy system. Copyright \uffc2\uffa9 2012 Society of Chemical Industry

", "keywords": ["2. Zero hunger", "0106 biological sciences", "China", "Reproduction", "Population Dynamics", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Pheromones", "Soil", "Echinochloa", "Rhizosphere", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology"], "contacts": [{"organization": "Chui-Hua Kong, Peng Wang, Xiang-Rui Meng, Hanwen Ni, Ming-Li Wang,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1002/ps.3380"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Pest%20Management%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ps.3380", "name": "item", "description": "10.1002/ps.3380", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ps.3380"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-08-07T00:00:00Z"}}, {"id": "10.1002/ps.7961", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:10Z", "type": "Journal Article", "created": "2024-01-20", "title": "The effect of natural products used as pesticides on the soil microbiota: OECD 216 nitrogen transformation test fails to identify effects that were detected viaq\u2010PCR microbial abundance measurement", "description": "AbstractBACKGROUND

Natural products present an environmentally attractive alternative to synthetic pesticides which have been implicated in the off\uffe2\uff80\uff90target effect. Currently, the assessment of pesticide toxicity on soil microorganisms relies on the OECD 216 N transformation assay (OECD stands for the Organisation Economic Co\uffe2\uff80\uff90operation and Development, which is a key international standard\uffe2\uff80\uff90setting organisation). We tested the hypotheses that (i) the OECD 216 assay fails to identify unacceptable effects of pesticides on soil microbiota compared to more advanced molecular and standardized tests, and (ii) the natural products tested (dihydrochalcone, isoflavone, aliphatic phenol, and spinosad) are less toxic to soil microbiota compared to a synthetic pesticide compound (3,5\uffe2\uff80\uff90dichloraniline). We determined the following in three different soils: (i) ammonium (NH4+) and nitrate (NO3\uffe2\uff88\uff92) soil concentrations, as dictated by the OECD 216 test, and (ii) the abundance of phylogenetically (bacteria and fungi) and functionally distinct microbial groups [ammonia\uffe2\uff80\uff90oxidizing archaea (AOA) and bacteria (AOB)] using quantitative polymerase chain reaction (q\uffe2\uff80\uff90PCR).

RESULTS

All pesticides tested exhibited limited persistence, with spinosad demonstrating the highest persistence. None of the pesticides tested showed clear dose\uffe2\uff80\uff90dependent effects on NH4+ and NO3\uffe2\uff88\uff92 levels and the observed effects were <25% of the control, suggesting no unacceptable impacts on soil microorganisms. In contrast, q\uffe2\uff80\uff90PCR measurements revealed (i) distinct negative effects on the abundance of total bacteria and fungi, which were though limited to one of the studied soils, and (ii) a significant reduction in the abundance of both AOA and AOB across soils. This reduction was attributed to both natural products and 3,5\uffe2\uff80\uff90dichloraniline.

CONCLUSION

Our findings strongly advocate for a revision of the current regulatory framework regarding the toxicity of pesticides to soil microbiota, which should integrate advanced and well\uffe2\uff80\uff90standardized tools. \uffc2\uffa9 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.1 mol% of total biomass showed statistically significant treatment responses. PCA ordinated forest types and treatment responses along three axes (36%, 26%, 12% of variance). EEA factors 1 and 2 correlated negatively with PLFA factor 1 ( r = -0.20 and -0.35, respectively, n = 108) and positively with PLFA factor 3 ( r = +0.36 and +0.20, respectively, n = 108). In general, EEA responses were more strongly tied to changes in bacterial PLFA than to changes in fungal PLFA. Collectively, our data suggests that N inhibition of oxidative activity involves more than the repression of ligninase expression by white-rot basidiomycetes.", "keywords": ["Michigan", "Nitrogen", "Science", "Ecology and Evolutionary Biology", "Nature Conservation", "Microbiology", "Trees", "Soil", "Geoecology/Natural Processes", "Health Sciences", "Cellular and Developmental Biology", "Ecosystem", "Phospholipids", "Soil Microbiology", "2. Zero hunger", "Analysis of Variance", "Principal Component Analysis", "Ecology", "Life Sciences", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "15. Life on land", "Enzymes", "13. Climate action", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1007/s00248-003-9001-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-003-9001-x", "name": "item", "description": "10.1007/s00248-003-9001-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-003-9001-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-04-19T00:00:00Z"}}, {"id": "10.1007/s00128-012-0523-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:22Z", "type": "Journal Article", "created": "2012-01-25", "title": "Effect Of Long-Term Zinc Pollution On Soil Microbial Community Resistance To Repeated Contamination", "description": "The aim of the study was to compare the effects of stress (contamination trials) on the microorganisms in zinc-polluted soil (5,018\u00a0mg Zn\u00a0kg(-1) soil dry weight) and unpolluted soil (141\u00a0mg Zn kg(-1) soil\u00a0dw), measured as soil respiration rate. In the laboratory, soils were subjected to copper contamination (0, 500, 1,500 and 4,500\u00a0mg\u00a0kg(-1) soil\u00a0dw), and then a bactericide (oxytetracycline) combined with a fungicide (captan) along with glucose (10\u00a0mg\u00a0g(-1) soil\u00a0dw each) were added. There was a highly significant effect of soil type, copper treatment and oxytetracycline/captan treatment. The initial respiration rate of chronically zinc-polluted soil was higher than that of unpolluted soil, but in the copper treatment it showed a greater decline. Microorganisms in copper-treated soil were more susceptible to oxytetracycline/captan contamination. After the successive soil contamination trials the decline of soil respiration was greater in zinc-polluted soil than in unpolluted soil.", "keywords": ["Health", " Toxicology and Mutagenesis", "trace metals", "Oxytetracycline", "Toxicology", "01 natural sciences", "Article", "Captan", "Soil", "Stress", " Physiological", "Soil Pollutants", "Soil Microbiology", "combined stressors", "0105 earth and related environmental sciences", "soil pollution", "Drug Resistance", " Microbial", "04 agricultural and veterinary sciences", "Pollution", "Adaptation", " Physiological", "soil respiration rate", "6. Clean water", "Anti-Bacterial Agents", "Fungicides", " Industrial", "Zinc", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Copper"], "contacts": [{"organization": "Klimek, Beata", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s00128-012-0523-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bulletin%20of%20Environmental%20Contamination%20and%20Toxicology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00128-012-0523-0", "name": "item", "description": "10.1007/s00128-012-0523-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00128-012-0523-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-01-26T00:00:00Z"}}, {"id": "10.1007/s00128-007-9045-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:22Z", "type": "Journal Article", "created": "2007-04-04", "title": "Zinc And Copper Toxicity To Soil Bacteria And Fungi From Zinc Polluted And Unpolluted Soils: A Comparative Study With Different Types Of Biolog Plates", "keywords": ["Microbiological Techniques", "Zinc", "Bacteria", "Fungi", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Poland", "04 agricultural and veterinary sciences", "01 natural sciences", "Copper", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00128-007-9045-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bulletin%20of%20Environmental%20Contamination%20and%20Toxicology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00128-007-9045-6", "name": "item", "description": "10.1007/s00128-007-9045-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00128-007-9045-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-04-05T00:00:00Z"}}, {"id": "10.1007/s00248-009-9508-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2009-03-30", "title": "Comparative Resistance And Resilience Of Soil Microbial Communities And Enzyme Activities In Adjacent Native Forest And Agricultural Soils", "description": "Degradation of soil properties following deforestation and long-term soil cultivation may lead to decreases in soil microbial diversity and functional stability. In this study, we investigated the differences in the stability (resistance and resilience) of microbial community composition and enzyme activities in adjacent soils under either native tropical forest (FST) or in agricultural cropping use for 14 years (AGR). Mineral soil samples (0 to 5 cm) from both areas were incubated at 40 degrees C, 50 degrees C, 60 degrees C, or 70 degrees C for 15 min in order to successively reduce the microbial biomass. Three and 30 days after the heat shocks, fluorescein diacetate (FDA) hydrolysis, cellulase and laccase activities, and phospholipid-derived fatty acids-based microbial community composition were measured. Microbial biomass was reduced up to 25% in both soils 3 days after the heat shocks. The higher initial values of microbial biomass, enzyme activity, total and particulate soil organic carbon, and aggregate stability in the FST soil coincided with higher enzymatic stability after heat shocks. FDA hydrolysis activity was less affected (more resistance) and cellulase and laccase activities recovered more rapidly (more resilience) in the FST soil relative to the AGR counterpart. In the AGR soil, laccase activity did not show resilience to any heat shock level up to 30 days after the disturbance. Within each soil type, the microbial community composition did not differ between heat shock and control samples at day 3. However, at day 30, FST soil samples treated at 60 degrees C and 70 degrees C contained a microbial community significantly different from the control and with lower biomass regardless of high enzyme resilience. Results of this study show that deforestation followed by long-term cultivation changed microbial community composition and had differential effects on microbial functional stability. Both soils displayed similar resilience to FDA hydrolysis, a composite measure of a broad range of hydrolases, supporting the concept of high functional redundancy in soil microbial communities. In contrast, the resilience of the substrate-specific activities of laccase and cellulase were lower in AGR soils, indicating a less diverse community of microorganisms capable of producing these enzymes and confirming that specific microbial functions are more sensitive measurements for evaluating change in the ecological stability of soils.", "keywords": ["2. Zero hunger", "Hot Temperature", "Laccase", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Fluoresceins", "6. Clean water", "Trees", "Soil", "Cellulase", "Enzyme Stability", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Brazil", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-009-9508-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-009-9508-x", "name": "item", "description": "10.1007/s00248-009-9508-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-009-9508-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-03-31T00:00:00Z"}}, {"id": "10.1007/s00248-005-5156-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:22Z", "type": "Journal Article", "created": "2006-01-02", "title": "Microbial Responses To Long-Term N Deposition In A Semiarid Grassland", "description": "Nitrogen (N) enrichment of the biosphere is an expanding problem to which arid ecosystems may be particularly sensitive. In semiarid grasslands, scarce precipitation uncouples plant and microbial activities, and creates within the soil a spatial mosaic of rhizosphere and cyanobacterial crust communities. We investigated the impact of elevated N deposition on these soil microbial communities at a grama-dominated study site located incentral New Mexico (USA). The study plots were established in 1995 and receive 10 kg ha(-1) year(-1) of supplemental N in the form of NH(4)NO(3). Soil samples were collected in July 2004, following 2 years of severe drought, and again in March 2005 following a winter of record high precipitation. Soils were assayed for potential activities of 20 extracellular enzymes and N(2)O production. The rhizosphere and crust-associated soils had peptidase and peroxidase potentials that were extreme in relation to those of temperate soils. N addition enhanced glycosidase and phosphatase activities and depressed peptidase. In contrast to temperate forest soils, oxidative enzyme activity did not respond to N treatment. Across sampling dates, extracellular enzyme activity responses correlated with inorganic N concentrations. N(2)O generation did not vary significantly with soil cover or N treatment. Microbial responses to N deposition in this semiarid grassland were distinct from those of forest ecosystems and appear to be modulated by inorganic N accumulation, which is linked to precipitation patterns.", "keywords": ["2. Zero hunger", "Principal Component Analysis", "Ecology", "Nitrogen", "Climate", "beta-Glucosidase", "Nitrous Oxide", "04 agricultural and veterinary sciences", "15. Life on land", "Poaceae", "01 natural sciences", "Carbon", "6. Clean water", "Enzymes", "Trees", "Leucyl Aminopeptidase", "Soil", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Soil Microbiology", "Peroxidase", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00248-005-5156-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-005-5156-y", "name": "item", "description": "10.1007/s00248-005-5156-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-005-5156-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-01-01T00:00:00Z"}}, {"id": "10.1007/s00248-007-9276-4", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:22Z", "type": "Journal Article", "created": "2007-07-17", "title": "Comparison Of Soil Bacterial Communities Under Diverse Agricultural Land Management And Crop Production Practices", "description": "The composition and structure of bacterial communities were examined in soil subjected to a range of diverse agricultural land management and crop production practices. Length heterogeneity polymerase chain reaction (LH-PCR) of bacterial DNA extracted from soil was used to generate amplicon profiles that were analyzed with univariate and multivariate statistical methods. Five land management programs were initiated in July 2000: conventional, organic, continuous removal of vegetation (disk fallow), undisturbed (weed fallow), and bahiagrass pasture (Paspalum notatum var Argentine). Similar levels in the diversity of bacterial 16S rDNA amplicons were detected in soil samples collected from organically and conventionally managed plots 3 and 4 years after initiation of land management programs, whereas significantly lower levels of diversity were observed in samples collected from bahiagrass pasture. Differences in diversity were attributed to effects on how the relative abundance of individual amplicons were distributed (evenness) and not on the total numbers of bacterial 16S rDNA amplicons detected (richness). Similar levels of diversity were detected among all land management programs in soil samples collected after successive years of tomato (Lycopersicon esculentum) cultivation. A different trend was observed after a multivariate examination of the similarities in genetic composition among soil bacterial communities. After 3 years of land management, similarities in genetic composition of soil bacterial communities were observed in plots where disturbance was minimized (bahiagrass and weed fallow). The genetic compositions in plots managed organically were similar to each other and distinct from bacterial communities in other land management programs. After successive years of tomato cultivation and damage from two major hurricanes, only the composition of soil bacterial communities within organically managed plots continued to maintain a high degree of similarity to each other and remain distinct from other bacterial communities. This study reveals the effects of agricultural land management practices on soil bacterial community composition and diversity in a large-scale, long-term replicated study where the effect of soil type on community attributes was removed.", "keywords": ["Crops", " Agricultural", "DNA", " Bacterial", "2. Zero hunger", "Analysis of Variance", "Conservation of Natural Resources", "Bacteria", "Agriculture", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "15. Life on land", "DNA", " Ribosomal", "Polymerase Chain Reaction", "Solanum lycopersicum", "RNA", " Ribosomal", " 16S", "0401 agriculture", " forestry", " and fisheries", "Cloning", " Molecular", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9276-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9276-4", "name": "item", "description": "10.1007/s00248-007-9276-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9276-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-07-07T00:00:00Z"}}, {"id": "10.1007/s00248-007-9295-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2007-08-31", "title": "Plfa Profiling Of Microbial Community Structure And Seasonal Shifts In Soils Of A Douglas-Fir Chronosequence", "description": "The impact and frequency of forest harvesting could significantly affect soil microbial community (SMC) structure and functioning. The ability of soil microorganisms to perform biogeochemical processes is critical for sustaining forest productivity and has a direct impact on decomposition dynamics and carbon storage potential. The Wind River Canopy Crane Research Forest in SW, WA, provided a unique opportunity to study a forest chronosequence and the residual effects of harvesting on the SMC in comparison to old-growth forests. The objective of this study was to determine the effect of clear-cutting and stand age on temporal dynamics of SMC and physiological stress markers using phospholipid fatty acid (PLFA) profiling. Soil microbial PLFA profiles were determined seven times over 22 months (Nov. 02 to Sep. 04) in old-growth coniferous forest stands (300-500 years) and 8 (CC8)- or 25 (CC25)-year-old replanted clear-cuts. PLFA patterns of the SMC shifted because of clear-cutting, but seasonal temporal changes had greater shifts than differences among stand age. The microbial biomass (total PLFA) and bacterial, fungal, and selected other PLFAs were significantly reduced in CC8 but not in CC25 sites relative to the old-growth sites. An increase in stress indicators [PLFA ratios of saturated/monsaturated and (cy17:0 + cy19:0)/(16:1omega7 + 18:1omega7)] in late summer was related to water stress. Although the canopy and litter input are quite different for a 25-year clear-cut compared to virgin old-growth forest, we conclude that the composition of the microbial communities, 25 years after clear-cutting, has recovered sufficiently to be much more similar to old-growth forests than a recent clear-cut at this Pacific Northwest forest site. The study shows the potential of PLFA analysis for profiling microbial communities and their stress status under field conditions, but wide temporal shifts emphasize the need for sampling over seasons to fully interpret ecosystem management impacts on microbial populations.", "keywords": ["Washington", "Time Factors", "Bacteria", "Fatty Acids", "Fungi", "Temperature", "Eukaryota", "Water", "04 agricultural and veterinary sciences", "15. Life on land", "Pseudotsuga", "Trees", "Soil", "13. Climate action", "Animals", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Seasons", "Phospholipids", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9295-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9295-1", "name": "item", "description": "10.1007/s00248-007-9295-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9295-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-31T00:00:00Z"}}, {"id": "10.1007/s00248-007-9308-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2007-09-01", "title": "Long-Term Effect Of Municipal Solid Waste Amendment On Microbial Abundance And Humus-Associated Enzyme Activities Under Semiarid Conditions", "description": "Microbial ecology is the key to understanding the function of soil biota for organic matter cycling after a single amendment of organic waste in semiarid soils. Therefore, in this paper, the long-term effect (17 years) of adding different doses of a solid municipal waste to an arid soil on humus-enzyme complexes, a very stable and long-lasting fraction of soil enzymes, as well as on microbial and plant abundance, was studied. Humic substances were extracted by 0.1 M pH 7 sodium pyrophosphate from soil samples collected in experimental plots amended with different doses of a solid municipal waste (0, 65, 130, 195, and 260 t/ha) 17 years before. The activity of different hydrolases related with the C (beta-glucosidase), N (urease), and P (alkaline phosphatase) cycles and with the formation of humic substances (o-diphenol oxidase) were determined in this extract. The density and diversity of plant cover in the plots, as well as the fungal and bacterial biomass (by analyzing phopholipid fatty acids) were also determined. In general, the amended plots showed greater humic substance-related enzymatic activity than the unamended plots. This activity increased with the dose but only up to a certain level, above which it leveled off or even diminished. Plant diversity and cover density followed the same trend. Fungal and bacterial biomass also benefited in a dose-dependent manner. Different signature molecules representing gram+ and gram- bacteria, and those corresponding to monounsaturated and saturated fatty acids showed a similar behavior. The results demonstrate that organic amendment had a noticeable long-term effect on the vegetal development, humic substances-related enzyme activity and on the development of bacteria and fungi in semiarid conditions.", "keywords": ["2. Zero hunger", "Analysis of Variance", "Time Factors", "Bacteria", "Fatty Acids", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "6. Clean water", "Enzymes", "Refuse Disposal", "12. Responsible consumption", "Soil", "Ergosterol", "11. Sustainability", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Humic Substances", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9308-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9308-0", "name": "item", "description": "10.1007/s00248-007-9308-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9308-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-09-02T00:00:00Z"}}, {"id": "10.1007/s00248-008-9467-7", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2008-11-21", "title": "Molecular And Functional Assessment Of Bacterial Community Convergence In Metal-Amended Soils", "description": "Species diversity and the structure of microbial communities in soils are thought to be a function of the cumulative selective pressures within the local environment. Shifts in microbial community structure, as a result of metal stress, may have lasting negative effects on soil ecosystem dynamics if critical microbial community functions are compromised. Three soils in the vicinity of a copper smelter, previously contaminated with background, low and high levels of aerially deposited metals, were amended with metal-salts to determine the potential for metal contamination to shape the structural and functional diversity of microbial communities in soils. We hypothesized that the microbial communities native to the three soils would initially be unique to each site, but would converge on a microbial community with similar structure and function, as a result of metal stress. Initially, the three different sites supported microbial communities with unique structural and functional diversity, and the nonimpacted site supported inherently higher levels of microbial activity and biomass, relative to the metal-contaminated sites. Amendment of the soils with metal-salts resulted in a decrease in microbial activity and biomass, as well as shifts in microbial community structure and function at each site. Soil microbial communities from each site were also observed to be sensitive to changes in soil pH as a result of metal-salt amendment; however, the magnitude of these pH-associated effects varied between soils. Microbial communities from each site did not converge on a structurally or functionally similar community following metal-salt amendment, indicating that other factors may be equally important in shaping microbial communities in soils. Among these factors, soil physiochemical parameters like organic matter and soil pH, which can both influence the bioavailability and toxicity of metals in soils, may be critical.", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "0303 health sciences", "Bacteria", "Biodiversity", "Hydrogen-Ion Concentration", "15. Life on land", "Soil", "03 medical and health sciences", "13. Climate action", "Metals", " Heavy", "Soil Pollutants", "Biomass", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-008-9467-7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-008-9467-7", "name": "item", "description": "10.1007/s00248-008-9467-7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-008-9467-7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-11-22T00:00:00Z"}}, {"id": "10.1007/s00248-010-9727-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2010-08-03", "title": "Soil Microbial Abundance And Diversity Along A Low Precipitation Gradient", "description": "The exploration of spatial patterns of abundance and diversity patterns along precipitation gradients has focused for centuries on plants and animals; microbial profiles along such gradients are largely unknown. We studied the effects of soil pH, nutrient concentration, salinity, and water content on bacterial abundance and diversity in soils collected from Mediterranean, semi-arid, and arid sites receiving approximately 400, 300, and 100 mm annual precipitation, respectively. Bacterial diversity was evaluated by terminal restriction fragment length polymorphism and clone library analyses and the patterns obtained varied with the climatic regions. Over 75% of the sequenced clones were unique to their environment, while \u223c2% were shared by all sites, yet, the Mediterranean and semi-arid sites had more common clones (\u223c9%) than either had with the arid site (4.7% and 6%, respectively). The microbial abundance, estimated by phospholipid fatty acids and real-time quantitative PCR assays, was significantly lower in the arid region. Our results indicate that although soil bacterial abundance decreases with precipitation, bacterial diversity is independent of precipitation gradient. Furthermore, community composition was found to be unique to each ecosystem.", "keywords": ["DNA", " Bacterial", "2. Zero hunger", "0301 basic medicine", "Salinity", "0303 health sciences", "Bacteria", "Mediterranean Region", "Climate", "Rain", "Water", "Biodiversity", "Hydrogen-Ion Concentration", "15. Life on land", "Soil", "03 medical and health sciences", "Phospholipids", "Polymorphism", " Restriction Fragment Length", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-010-9727-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-010-9727-1", "name": "item", "description": "10.1007/s00248-010-9727-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-010-9727-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-01T00:00:00Z"}}, {"id": "10.1007/s00248-011-9897-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2011-06-29", "title": "Impacts Of Organic And Inorganic Fertilizers On Nitrification In A Cold Climate Soil Are Linked To The Bacterial Ammonia Oxidizer Community", "description": "The microbiology underpinning soil nitrogen cycling in northeast China remains poorly understood. These agricultural systems are typified by widely contrasting temperature, ranging from -40 to 38\u00b0C. In a long-term site in this region, the impacts of mineral and organic fertilizer amendments on potential nitrification rate (PNR) were determined. PNR was found to be suppressed by long-term mineral fertilizer treatment but enhanced by manure treatment. The abundance and structure of ammonia-oxidizing bacterial (AOB) and archaeal (AOA) communities were assessed using quantitative polymerase chain reaction and denaturing gradient gel electrophoresis techniques. The abundance of AOA was reduced by all fertilizer treatments, while the opposite response was measured for AOB, leading to a six- to 60-fold reduction in AOA/AOB ratio. The community structure of AOA exhibited little variation across fertilization treatments, whereas the structure of the AOB community was highly responsive. PNR was correlated with community structure of AOB rather than that of AOA. Variation in the community structure of AOB was linked to soil pH, total carbon, and nitrogen contents induced by different long-term fertilization regimes. The results suggest that manure amendment establishes conditions which select for an AOB community type which recovers mineral fertilizer-suppressed soil nitrification.", "keywords": ["DNA", " Bacterial", "2. Zero hunger", "China", "Bacteria", "04 agricultural and veterinary sciences", "15. Life on land", "Cold Climate", "Archaea", "Nitrification", "6. Clean water", "Genes", " Archaeal", "Soil", "DNA", " Archaeal", "Ammonia", "Genes", " Bacterial", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Fertilizers", "Oxidoreductases", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-011-9897-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-011-9897-5", "name": "item", "description": "10.1007/s00248-011-9897-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-011-9897-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-06-29T00:00:00Z"}}, {"id": "10.1007/s00248-013-0225-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2013-04-15", "title": "Agricultural Management And Labile Carbon Additions Affect Soil Microbial Community Structure And Interact With Carbon And Nitrogen Cycling", "description": "We investigated how conversion from conventional agriculture to organic management affected the structure and biogeochemical function of soil microbial communities. We hypothesized the following. (1) Changing agricultural management practices will alter soil microbial community structure driven by increasing microbial diversity in organic management. (2) Organically managed soil microbial communities will mineralize more N and will also mineralize more N in response to substrate addition than conventionally managed soil communities. (3) Microbial communities under organic management will be more efficient and respire less added C. Soils from organically and conventionally managed agroecosystems were incubated with and without glucose ((13)C) additions at constant soil moisture. We extracted soil genomic DNA before and after incubation for TRFLP community fingerprinting of soil bacteria and fungi. We measured soil C and N pools before and after incubation, and we tracked total C respired and N mineralized at several points during the incubation. Twenty years of organic management altered soil bacterial and fungal community structure compared to continuous conventional management with the bacterial differences caused primarily by a large increase in diversity. Organically managed soils mineralized twice as much NO3 (-) as conventionally managed ones (44 vs. 23 \u03bcg N/g soil, respectively) and increased mineralization when labile C was added. There was no difference in respiration, but organically managed soils had larger pools of C suggesting greater efficiency in terms of respiration per unit soil C. These results indicate that the organic management induced a change in community composition resulting in a more diverse community with enhanced activity towards labile substrates and greater capacity to mineralize N.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Environmental Microbiology and Microbial Ecology", "Bacteria", "Nitrogen", "Fungal Community Structure", "Agriculture", "Nitrogen Cycle", "15. Life on land", "Microbiology", "630", "Carbon", "Carbon Cycle", "Soil", "03 medical and health sciences", "rRNA Gene Copy", "Soil Microbial Community Structure", "fungal community", "Biology", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-013-0225-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-013-0225-0", "name": "item", "description": "10.1007/s00248-013-0225-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-013-0225-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-16T00:00:00Z"}}, {"id": "10.1007/s00248-013-0235-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2013-05-01", "title": "Changes In Diversity, Abundance, And Structure Of Soil Bacterial Communities In Brazilian Savanna Under Different Land Use Systems", "description": "The Brazilian Savanna, also known as 'Cerrado', is the richest and most diverse savanna in the world and has been ranked as one of the main hotspots of biodiversity. The Cerrado is a representative biome in Central Brazil and the second largest biome in species diversity of South America. Nevertheless, large areas of native vegetation have been converted to agricultural land including grain production, livestock, and forestry. In this view, understanding how land use affects microbial communities is fundamental for the sustainable management of agricultural ecosystems. The aim of this work was to analyze and compare the soil bacterial communities from the Brazilian Cerrado associated with different land use systems using high throughput pyrosequencing of 16S rRNA genes. Relevant differences were observed in the abundance and structure of bacterial communities in soils under different land use systems. On the other hand, the diversity of bacterial communities was not relevantly changed among the sites studied. Land use systems had also an important impact on specific bacterial groups in soil, which might change the soil function and the ecological processes. Acidobacteria, Proteobacteria, and Actinobacteria were the most abundant groups in the Brazilian Cerrado. These findings suggest that more important than analyzing the general diversity is to analyze the composition of the communities. Since soil type was the same among the sites, we might assume that land use was the main factor defining the abundance and structure of bacterial communities.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Bacteria", "Molecular Sequence Data", "Agriculture", "Biodiversity", "15. Life on land", "Brazil", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-013-0235-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-013-0235-y", "name": "item", "description": "10.1007/s00248-013-0235-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-013-0235-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-27T00:00:00Z"}}, {"id": "10.1007/s00248-013-0322-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2013-11-25", "title": "Pyrosequencing Reveals Contrasting Soil Bacterial Diversity And Community Structure Of Two Main Winter Wheat Cropping Systems In China", "description": "Microbes are key components of the soil environment, playing an important role in maintaining soil health, sustainability, and productivity. The composition and structure of soil bacterial communities were examined in winter wheat-rice (WR) and winter wheat-maize (WM) cropping systems derived from five locations in the Low-Middle Yangtze River plain and the Huang-Huai-Hai plain by pyrosequencing of the 16S ribosomal RNA gene amplicons. A total of 102,367 high quality sequences were used for multivariate statistical analysis and to test for correlation between community structure and environmental variables such as crop rotations, soil properties, and locations. The most abundant phyla across all soil samples were Proteobacteria, Acidobacteria, and Bacteroidetes. Similar patterns of bacterial diversity and community structure were observed within the same cropping systems, and a higher relative abundance of anaerobic bacteria was found in WR compared to WM cropping systems. Variance partitioning analysis revealed complex relationships between bacterial community and environmental variables. The effect of crop rotations was low but significant, and interactions among soil properties, locations, and crop rotations accounted for most of the explained variation in the structure of bacterial communities. Soil properties such as pH, available P, and available K showed higher correlations (positive or negative) with the majority of the abundant taxa. Bacterial diversity (the Shannon index) and richness (Chao1 and ACE) were higher under WR than WM cropping systems.", "keywords": ["Crops", " Agricultural", "DNA", " Bacterial", "0301 basic medicine", "2. Zero hunger", "China", "0303 health sciences", "Agriculture", "Biodiversity", "Sequence Analysis", " DNA", "15. Life on land", "Zea mays", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "11. Sustainability", "Soil Microbiology", "Triticum"]}, "links": [{"href": "https://doi.org/10.1007/s00248-013-0322-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-013-0322-0", "name": "item", "description": "10.1007/s00248-013-0322-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-013-0322-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-11-26T00:00:00Z"}}, {"id": "10.1007/s00248-016-0730-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2016-02-02", "title": "Responses Of Soil Bacterial Communities To Nitrogen Deposition And Precipitation Increment Are Closely Linked With Aboveground Community Variation", "description": "It has been predicted that precipitation and atmospheric nitrogen (N) deposition will increase in northern China; yet, ecosystem responses to the interactive effects of water and N remain largely unknown. In particular, responses of belowground microbial community to projected global change and their potential linkages to aboveground macro-organisms are rarely studied. In this study, we examined the responses of soil bacterial diversity and community composition to increased precipitation and multi-level N deposition in a temperate steppe in Inner Mongolia, China, and explored the diversity linkages between aboveground and belowground communities. It was observed that N addition caused the significant decrease in bacterial alpha-diversity and dramatic changes in community composition. In addition, we documented strong correlations of alpha- and beta-diversity between plant and bacterial communities in response to N addition. It was found that N enriched the so-called copiotrophic bacteria, but reduced the oligotrophic groups, primarily by increasing the soil inorganic N content and carbon availability and decreasing soil pH. We still highlighted that increased precipitation tended to alleviate the effects of N on bacterial diversity and dampen the plant-microbe connections induced by N. The counteractive effects of N addition and increased precipitation imply that even though the ecosystem diversity and function are predicted to be negatively affected by N deposition in the coming decades; the combination with increased precipitation may partially offset this detrimental effect.", "keywords": ["0301 basic medicine", "2. Zero hunger", "China", "0303 health sciences", "Bacteria", "Nitrogen", "Climate", "Microbial Consortia", "Water", "Biodiversity", "Plants", "15. Life on land", "Carbon", "6. Clean water", "Soil", "03 medical and health sciences", "13. Climate action", "Chemical Precipitation", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-016-0730-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-016-0730-z", "name": "item", "description": "10.1007/s00248-016-0730-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-016-0730-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-02T00:00:00Z"}}, {"id": "10.1007/s00248-018-1305-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2018-12-08", "title": "Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts", "description": "Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment. So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures. However, the characteristics of the cyanobacterial polysaccharidic matrix may greatly depend on soil type. The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types. S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS). LB-EPSs were mainly composed of low MW molecules (<\u200950\u00a0kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000\u00a0kDa). The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents. In more C-rich soils, high abundances of galactose, mannose, and xylose were also found. Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils. Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Cyanobacteria inoculation", "Tightly bound EPS", "Polysaccharides", " Bacterial", "Biological soil crust; Cyanobacteria inoculation; Loosely bound EPS; Molecular weight; Monosaccharide composition; Tightly bound EPS; Ecology; Evolution; Behavior and Systematics; Ecology; Soil Science", "Biological soil crust", "Monosaccharide composition", "15. Life on land", "Cyanobacteria", "Molecular weight", "Biological soil crust; Cyanobacteria inoculation; Loosely bound EPS; Molecular weight; Monosaccharide composition; Tightly bound EPS; Ecology", " Evolution", " Behavior and Systematics; Ecology; Soil Science", "Soil", "03 medical and health sciences", "13. Climate action", "Loosely bound EPS", "Desert Climate", "Soil Microbiology"]}, "links": [{"href": "https://iris.unive.it/bitstream/10278/5089943/1/s00248-018-1305-y.pdf"}, {"href": "http://link.springer.com/content/pdf/10.1007/s00248-018-1305-y.pdf"}, {"href": "https://doi.org/10.1007/s00248-018-1305-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-018-1305-y", "name": "item", "description": "10.1007/s00248-018-1305-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-018-1305-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-08T00:00:00Z"}}, {"id": "10.1007/s00253-011-3535-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2011-08-17", "title": "Methanotrophic Community Structure And Activity Under Warming And Grazing Of Alpine Meadow On The Tibetan Plateau", "description": "Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH(4) under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were studied in an alpine meadow soil on the Tibetan Plateau. There was high abundance of methanotrophs (1.2-3.4\u2009\u00d7\u200910(8) pmoA gene copies per gram of dry weight soil) assessed by real-time PCR, and warming significantly increased the abundance regardless of grazing. A total of 64 methanotrophic operational taxonomic units (OTUs) were obtained from 1,439 clone sequences, of these OTUs; 63 OTUs (98.4%) belonged to type I methanotrophs, and only one OTU was Methylocystis of type II methanotrophs. The methanotroph community composition and diversity were not apparently affected by the treatments. Warming and grazing significantly enhanced the potential CH(4) oxidation activity. There were significantly negative correlations between methanotrophic abundance and soil moisture and between methanotrophic abundance and NH(4)-N content. The study suggests that type I methanotrophs, as the dominance, may play a key role in CH(4) oxidation, and the alpine meadow has great potential to consume more CH(4) under future warmer and grazing conditions on the Tibetan Plateau.", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Sheep", "Bacteria", "Molecular Sequence Data", "Temperature", "Sequence Analysis", " DNA", "15. Life on land", "Real-Time Polymerase Chain Reaction", "Tibet", "Biota", "Soil", "03 medical and health sciences", "Ammonia", "13. Climate action", "Animals", "Methane", "Oxidation-Reduction", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00253-011-3535-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Microbiology%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00253-011-3535-5", "name": "item", "description": "10.1007/s00253-011-3535-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00253-011-3535-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-08-17T00:00:00Z"}}, {"id": "10.1007/s00253-016-7736-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:23Z", "type": "Journal Article", "created": "2016-07-27", "title": "Structure Of Bacterial Communities In Soil Following Cover Crop And Organic Fertilizer Incorporation", "description": "Incorporation of organic material into soils is an important element of organic farming practices that can affect the composition of the soil bacterial communities that carry out nutrient cycling and other functions crucial to crop health and growth. We conducted a field experiment to determine the effects of cover crops and fertilizers on bacterial community structure in agricultural soils under long-term organic management. Illumina sequencing of 16S rDNA revealed diverse communities comprising 45 bacterial phyla in corn rhizosphere and bulk field soil. Community structure was most affected by location and by the rhizosphere effect, followed by sampling time and amendment treatment. These effects were associated with soil physicochemical properties, including pH, moisture, organic matter, and nutrient levels. Treatment differences were apparent in bulk and rhizosphere soils at the time of peak corn growth in the season following cover crop and fertilizer application. Cover crop and fertilizer treatments tended to lower alpha diversity in early season samples. However, winter rye, oilseed radish, and buckwheat cover crop treatments increased alpha diversity in some later season samples compared to a no-amendment control. Fertilizer treatments and some cover crops decreased relative abundance of members of the ammonia-oxidizing family Nitrosomonadaceae. Pelleted poultry manure and Sustane\u00ae (a commercial fertilizer) decreased the relative abundance of Rhizobiales. Our data point to a need for future research exploring how (1) cover crops influence bacterial community structure and functions, (2) these effects differ with biomass composition and quantity, and (3) existing soil conditions and microbial community composition influence how soil microbial populations respond to agricultural management practices.", "keywords": ["Crops", " Agricultural", "DNA", " Bacterial", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "High-Throughput Nucleotide Sequencing", "Sequence Analysis", " DNA", "15. Life on land", "Biota", "DNA", " Ribosomal", "03 medical and health sciences", "13. Climate action", "RNA", " Ribosomal", " 16S", "Fertilizers", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00253-016-7736-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Microbiology%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00253-016-7736-9", "name": "item", "description": "10.1007/s00253-016-7736-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00253-016-7736-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-07-27T00:00:00Z"}}, {"id": "10.1007/s00267-009-9319-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:24Z", "type": "Journal Article", "created": "2009-06-15", "title": "Field Trial Assessment Of Biological, Chemical, And Physical Responses Of Soil To Tillage Intensity, Fertilization, And Grazing", "description": "Soil microbial populations can fluctuate in response to environmental changes and, therefore, are often used as biological indicators of soil quality. Soil chemical and physical parameters can also be used as indicators because they can vary in response to different management strategies. A long-term field trial was conducted to study the effects of different tillage systems (NT: no tillage, DH: disc harrow, and MP: moldboard plough), P fertilization (diammonium phosphate), and cattle grazing (in terms of crop residue consumption) in maize (Zea mays L.), sunflower (Heliantus annuus L.), and soybean (Glycine max L.) on soil biological, chemical, and physical parameters. The field trial was conducted for four crop years (2000/2001, 2001/2002, 2002/2003, and 2003/2004). Soil populations of Actinomycetes, Trichoderma spp., and Gliocladium spp. were 49% higher under conservation tillage systems, in soil amended with diammonium phosphate (DAP) and not previously grazed. Management practices also influenced soil chemical parameters, especially organic matter content and total N, which were 10% and 55% higher under NT than under MP. Aggregate stability was 61% higher in NT than in MP, 15% higher in P-fertilized soil, and also 9% higher in not grazed strips, bulk density being 12% lower in NT systems compared with MP. DAP application and the absence of grazing also reduced bulk density (3%). Using conservation tillage systems, fertilizing crops with DAP, and avoiding grazing contribute to soil health preservation and enhanced crop production.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Soil", "13. Climate action", "Animals", "0401 agriculture", " forestry", " and fisheries", "Agriculture", "Cattle", "04 agricultural and veterinary sciences", "15. Life on land", "Soil Microbiology", "6. Clean water", "Environmental Monitoring"], "contacts": [{"organization": "M. Zuza, Claudio Oddino, G. J. March, A. Marinelli, Anal\u00eda Becker, Silvina Vargas Gil,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s00267-009-9319-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00267-009-9319-3", "name": "item", "description": "10.1007/s00267-009-9319-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00267-009-9319-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-06-16T00:00:00Z"}}, {"id": "10.1007/s00267-010-9504-4", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:24Z", "type": "Journal Article", "created": "2010-05-27", "title": "Changes In Soil Particulate Organic Matter, Microbial Biomass, And Activity Following Afforestation Of Marginal Agricultural Lands In A Semi-Arid Area Of Northeast China", "description": "Afforestation of agricultural lands has been one of the major land use changes in China in recent decades. To better understand the effect of such land use change on soil quality, we investigated selected soil physical, chemical and microbial properties (0-15 cm depth) in marginal agricultural land and a chronosequence of poplar (Populus euramericana cv. 'N3016') plantations (5-, 10-, 15- and 20-years old) in a semi-arid area of Northeast China. Soil bulk density significantly declined after conversion of agricultural lands to poplar plantations. Soil total organic carbon (TOC) and nitrogen (TN) concentrations, microbial biomass C (MBC) and potential N mineralization rate (PNM) decreased initially following afforestation of agricultural lands, and then increased with stand development. However, soil metabolic quotient (qCO(2)) exhibited a reverse trend. In addition, soil particulate organic matter C (POM-C) and N (POM-N) concentrations showed no significant changes in the first 10 years following afforestation, and then increased with stand age. These findings demonstrated that soil quality declined initially following afforestation of agricultural lands in semi-arid regions, and then recovered with stand development. Following 15 years of afforestation, many soil quality parameters recovered to the values found in agricultural land. We propose that change in soil quality with stand age should be considered in determining optimum rotation length of plantations and best management practices for afforestation programs.", "keywords": ["2. Zero hunger", "China", "Nitrogen", "Agriculture", "Forestry", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "Soil", "Populus", "0401 agriculture", " forestry", " and fisheries", "Particulate Matter", "Biomass", "Environmental Restoration and Remediation", "Soil Microbiology", "Environmental Monitoring"], "contacts": [{"organization": "Rong Mao, De-Hui Zeng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s00267-010-9504-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00267-010-9504-4", "name": "item", "description": "10.1007/s00267-010-9504-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00267-010-9504-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-05-28T00:00:00Z"}}, {"id": "10.1007/s00267-012-9890-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:24Z", "type": "Journal Article", "created": "2012-06-28", "title": "Changes In Labile Organic Carbon Fractions And Soil Enzyme Activities After Marshland Reclamation And Restoration In The Sanjiang Plain In Northeast China", "description": "The extensive reclamation of marshland into cropland has tremendously impacted the ecological environment of the Sanjiang Plain in northeast China. To understand the impacts of marshland reclamation and restoration on soil properties, we investigated the labile organic carbon fractions and the soil enzyme activities in an undisturbed marshland, a cultivated marshland and three marshlands that had been restored for 3, 6 and 12 years. Soil samples collected from the different management systems at a depth of 0-20 cm in July 2009 were analyzed for soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass carbon (MBC) and easily degradable organic carbon. In addition, the activities of the invertase, \u03b2-glucosidase, urease and acid phosphatase were determined. These enzymes are involved in C, N and P cycling, respectively. Long-term cultivation resulted in decreased SOC, DOC, MBC, microbial quotient and C (invertase, \u03b2-glucosidase) and N-transforming (urease) enzyme activities compared with undisturbed marshland. After marshland restoration, the MBC and DOC concentrations and the soil invertase, \u03b2-glucosidase and urease activities increased. Soil DOC and MBC concentrations are probably the main factors responsible for the different invertase, \u03b2-glucosidase and urease activities. In addition, marshland restoration caused a significant increase in the microbial quotient, which reflects enhanced efficiency of organic substrate use by microbial biomass. Our observations demonstrated that soil quality recovered following marshland restoration. DOC, MBC and invertase, \u03b2-glucosidase and urease activities were sensitive for discriminating soil ecosystems under the different types of land use. Thus, these parameters should be considered to be indicators for detecting changes in soil quality and environmental impacts in marshlands.", "keywords": ["2. Zero hunger", "China", "Conservation of Natural Resources", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "6. Clean water", "Enzymes", "Soil", "13. Climate action", "Wetlands", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00267-012-9890-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00267-012-9890-x", "name": "item", "description": "10.1007/s00267-012-9890-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00267-012-9890-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-06-29T00:00:00Z"}}, {"id": "10.1007/s00442-007-0836-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:32Z", "type": "Journal Article", "created": "2007-08-27", "title": "Microbial Responses To Nitrogen Addition In Three Contrasting Grassland Ecosystems", "description": "The effects of global N enrichment on soil processes in grassland ecosystems have received relatively little study. We assessed microbial community response to experimental increases in N availability by measuring extracellular enzyme activity (EEA) in soils from three grasslands with contrasting edaphic and climatic characteristics: a semiarid grassland at the Sevilleta National Wildlife Refuge, New Mexico, USA (SEV), and mesic grasslands at Konza Prairie, Kansas, USA (KNZ) and Ukulinga Research Farm, KwaZulu-Natal, South Africa (SAF). We hypothesized that, with N enrichment, soil microbial communities would increase C and P acquisition activity, decrease N acquisition activity, and reduce oxidative enzyme production (leading to recalcitrant soil organic matter [SOM] accumulation), and that the magnitude of response would decrease with soil age (due to higher stabilization of enzyme pools and P limitation of response). Cellulolytic activities followed the pattern predicted, increasing 35-52% in the youngest soil (SEV), 10-14% in the intermediate soil (KNZ) and remaining constant in the oldest soil (SAF). The magnitude of phosphatase response did not vary among sites. N acquisition activity response was driven by the enzyme closest to its pH optimum in each soil: i.e., leucine aminopeptidase in alkaline soil, beta-N-acetylglucosaminidase in acidic soil. Oxidative enzyme activity varied widely across ecosystems, but did not decrease with N amendment at any site. Likewise, SOM and %C pools did not respond to N enrichment. Between-site variation in both soil properties and EEA exceeded any treatment response, and a large portion of EEA variability (leucine aminopeptidase and oxidative enzymes), 68% as shown by principal components analysis, was strongly related to soil pH (r = 0.91, P < 0.001). In these grassland ecosystems, soil microbial responses appear constrained by a molecular-scale (pH) edaphic factor, making potential breakdown rates of SOM resistant to N enrichment.", "keywords": ["2. Zero hunger", "0106 biological sciences", "Analysis of Variance", "Bacteria", "Nitrogen", "New Mexico", "04 agricultural and veterinary sciences", "Kansas", "15. Life on land", "Poaceae", "01 natural sciences", "Carbon", "Enzymes", "Soil", "South Africa", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00442-007-0836-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-007-0836-6", "name": "item", "description": "10.1007/s00442-007-0836-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-007-0836-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-28T00:00:00Z"}}, {"id": "10.1007/s00442-002-1130-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:31Z", "type": "Journal Article", "created": "2014-12-22", "title": "Responses Of Soil Nitrogen Dynamics In A Mojave Desert Ecosystem To Manipulations In Soil Carbon And Nitrogen Availability", "description": "We investigated the effects of changes in soil C and N availability on N mineralization, nitrification, denitrification, NH(3) volatilization, and soil respiration in the Mojave Desert. Results indicate a C limitation to microbial N cycling. Soils from underneath the canopies of Larrea tridentata (DC.) Cov., Pleuraphis rigida Thurber, and Lycium spp. exhibited higher rates of CO(2 ) flux, lower rates of NH(3) volatilization, and a decrease in inorganic N (NH(4)(+)-N and NO(3)(-)-N) with C addition. In addition to C limitation, soils from plant interspaces also exhibited a N limitation. Soils from all locations had net immobilization of N over the course of a 15-day laboratory incubation. However, soils from interspaces had lower rates of net nitrification and potential denitrification compared to soils from under plant canopies. The response to changes in C availability appears to be a short-term increase in microbial immobilization of inorganic N. Under controlled conditions, and over a longer time period, the effects of C and N availability appear to give way to larger differences due to spatial location. These findings have implications for ecosystems undergoing changes in soil C and N availability due to such processes as desertification, exotic species invasions, or elevated atmospheric CO(2) concentration.", "keywords": ["Nitrogen", "Biological Availability", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "01 natural sciences", "California", "Carbon", "Soil", "Ammonia", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Desert Climate", "Volatilization", "Ecosystem", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00442-002-1130-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-002-1130-2", "name": "item", "description": "10.1007/s00442-002-1130-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-002-1130-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-01-25T00:00:00Z"}}, {"id": "10.1007/s00442-006-0381-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:31Z", "type": "Journal Article", "created": "2006-02-17", "description": "The aspen free-air CO2 and O3 enrichment (FACTS II-FACE) study in Rhinelander, Wisconsin, USA, is designed to understand the mechanisms by which young northern deciduous forest ecosystems respond to elevated atmospheric carbon dioxide (CO2) and elevated tropospheric ozone (O3) in a replicated, factorial, field experiment. Soil respiration is the second largest flux of carbon (C) in these ecosystems, and the objective of this study was to understand how soil respiration responded to the experimental treatments as these fast-growing stands of pure aspen and birch + aspen approached maximum leaf area. Rates of soil respiration were typically lowest in the elevated O3 treatment. Elevated CO2 significantly stimulated soil respiration (8-26%) compared to the control treatment in both community types over all three growing seasons. In years 6-7 of the experiment, the greatest rates of soil respiration occurred in the interaction treatment (CO2 + O3), and rates of soil respiration were 15-25% greater in this treatment than in the elevated CO2 treatment, depending on year and community type. Two of the treatments, elevated CO2 and elevated CO2 + O3, were fumigated with 13C-depleted CO2, and in these two treatments we used standard isotope mixing models to understand the proportions of new and old C in soil respiration. During the peak of the growing season, C fixed since the initiation of the experiment in 1998 (new C) accounted for 60-80% of total soil respiration. The isotope measurements independently confirmed that more new C was respired from the interaction treatment compared to the elevated CO2 treatment. A period of low soil moisture late in the 2003 growing season resulted in soil respiration with an isotopic signature 4-6 per thousand enriched in 13C compared to sample dates when the percentage soil moisture was higher. In 2004, an extended period of low soil moisture during August and early September, punctuated by a significant rainfall event, resulted in soil respiration that was temporarily 4-6 per thousand more depleted in 13C. Up to 50% of the Earth's forests will see elevated concentrations of both CO2 and O3 in the coming decades and these interacting atmospheric trace gases stimulated soil respiration in this study.", "keywords": ["0106 biological sciences", "Science", "Ecology and Evolutionary Biology", "Cell Respiration", "Acer", "Carbon Cycling", "Plant Roots", "01 natural sciences", "Trees", "Soil", "Ozone", "Stable Isotope", "Air Pollution", "Health Sciences", "\u03b4 13 C", "Global Change", "Cellular and Developmental Biology", "Betula", "Ecosystem", "Soil Microbiology", "Carbon Isotopes", "Atmosphere", "Natural Resources and Environment", "Molecular", "Carbon Dioxide", "15. Life on land", "Populus", "13. Climate action"]}, "links": [{"href": "https://doi.org/10.1007/s00442-006-0381-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-006-0381-8", "name": "item", "description": "10.1007/s00442-006-0381-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-006-0381-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-02-18T00:00:00Z"}}, {"id": "10.1007/s00442-003-1391-4", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:31Z", "type": "Journal Article", "created": "2003-12-10", "title": "Carbon Availability Controls The Growth Of Detritivores (Lumbricidae) And Their Effect On Nitrogen Mineralization", "description": "Activity of soil decomposer microorganisms is generally limited by carbon availability, but factors controlling saprophagous soil animals remain largely unknown. In contrast to microorganisms, animals are unable to exploit mineral nutrient pools. Therefore, it has been suggested that soil animals, and earthworms in particular, are limited by the availability of nitrogen. In contrast to this view, a strong increase in density and biomass of endogeic earthworms in response to labile organic carbon addition has been documented in field experiments. The hypothesis that the growth of endogeic earthworms is primarily limited by carbon availability was tested in a laboratory experiment lasting for 10 weeks. In addition, it was investigated whether the effects of earthworms on microbial activity and nutrient mineralization depend on the availability of carbon resources. We manipulated food availability to the endogeic earthworm species Octolasion tyrtaeum by using two soils with different organic matter content, providing access to different amounts of soil, and adding labile organic carbon (glucose) enriched in (13)C. Glucose addition strongly increased the growth of O. tyrtaeum. From 8 to 17% of the total C in earthworm tissue was assimilated from the glucose added. Soil microbial biomass was not strongly affected by the addition of glucose, though basal respiration was significantly increased and up to 50% of the carbon added as glucose was incorporated into soil organic matter. The impact of earthworms on the mineralization and leaching of nitrogen depended on C availability. As expected, in C-limited soil, the presence of earthworms strongly increased nitrogen leaching. However, when C availability was increased by the addition of glucose, this pattern was reversed, i.e. the presence of O. tyrtaeum decreased nitrogen leaching and its availability to soil microflora. We conclude that irrespective of the total carbon content of soils, O. tyrtaeum was primarily limited by carbon, and that increased carbon availability allowed earthworms to be more effective in mobilizing N. The presence of earthworms increases C limitation of soil microorganisms, due to increased availability of N and P in earthworm casts or a direct depletion of easily available carbon resources by earthworms.", "keywords": ["0106 biological sciences", "2. Zero hunger", "Carbon Isotopes", "Nitrogen", "Population Dynamics", "Biological Availability", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Carbon", "Soil", "Animals", "0401 agriculture", " forestry", " and fisheries", "Oligochaeta", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00442-003-1391-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-003-1391-4", "name": "item", "description": "10.1007/s00442-003-1391-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-003-1391-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-01-01T00:00:00Z"}}, {"id": "10.1007/s00442-004-1540-4", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:31Z", "type": "Journal Article", "created": "2004-03-19", "title": "Feedback Interactions Between Needle Litter Decomposition And Rhizosphere Activity", "description": "The aim of our study was to identify interactions between the decomposition of aboveground litter and rhizosphere activity. The experimental approach combined the placement of labelled litter (delta13C=-37.9 per thousand ) with forest girdling in a 35-year-old Norway spruce stand, resulting in four different treatment combinations: GL (girdled, litter), GNL (girdled, no litter), NGL (not girdled, litter), and NGNL (not girdled, no litter). Monthly sampling of soil CO2 efflux and delta13C of soil respired CO2 between May and October 2002 allowed the partitioning of the flux into that derived from the labelled litter, and that derived from native soil organic matter and roots. The effect of forest girdling on soil CO2 efflux was detectable from June (girdling took place in April), and resulted in GNL fluxes to be about 50% of NGNL fluxes by late August. The presence of litter resulted in significantly increased fluxes for the first 2 months of the experiment, with significantly greater litter derived fluxes from non-girdled plots and a significant interaction between girdling and litter treatments over the same period. For NGL collars, the additional efflux was found to originate only in part from litter decomposition, but also from the decay of native soil organic matter. In GL collars, this priming effect was not significant, indicating an active role of the rhizosphere in soil priming. The results therefore indicate mutual positive feedbacks between litter decomposition and rhizosphere activity. Soil biological analysis (microbial and fungal biomass) of the organic layers indicated greatest activity below NGL collars, and we suppose that this increase indicates the mechanism of mutual positive feedback between rhizosphere activity and litter decomposition. However, elimination of fresh C input from both above- and belowground (GNL) also resulted in greater fungal abundance than for the NGNL treatment, indicating likely changes in fungal community structure (i.e. a shift from symbiotic to saprotrophic species abundance).", "keywords": ["570", "Soil ecology", "Microbial biomass", "Models", " Biological", "630", "Soil", "Biomass", "Picea", "Forest girdling; Microbial biomass; Soil CO; 2; efflux; Soil organic matter; Stable C isotopes;", "Ecosystem", "Soil Microbiology", "Soil CO2 efflux", "Feedback", " Physiological", "Soil organic matter", "Carbon Isotopes", "Fungi", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Microbial growth", "Stable C isotopes", "Plant Leaves", "13. Climate action", "Soils", "0401 agriculture", " forestry", " and fisheries", "Forest girdling", "Seasons"]}, "links": [{"href": "https://doi.org/10.1007/s00442-004-1540-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-004-1540-4", "name": "item", "description": "10.1007/s00442-004-1540-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-004-1540-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-05-01T00:00:00Z"}}, {"id": "10.1007/s00442-004-1788-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:31Z", "type": "Journal Article", "created": "2005-02-01", "title": "Effects Of Fire On Properties Of Forest Soils: A Review", "description": "Many physical, chemical, mineralogical, and biological soil properties can be affected by forest fires. The effects are chiefly a result of burn severity, which consists of peak temperatures and duration of the fire. Climate, vegetation, and topography of the burnt area control the resilience of the soil system; some fire-induced changes can even be permanent. Low to moderate severity fires, such as most of those prescribed in forest management, promote renovation of the dominant vegetation through elimination of undesired species and transient increase of pH and available nutrients. No irreversible ecosystem change occurs, but the enhancement of hydrophobicity can render the soil less able to soak up water and more prone to erosion. Severe fires, such as wildfires, generally have several negative effects on soil. They cause significant removal of organic matter, deterioration of both structure and porosity, considerable loss of nutrients through volatilisation, ash entrapment in smoke columns, leaching and erosion, and marked alteration of both quantity and specific composition of microbial and soil-dwelling invertebrate communities. However, despite common perceptions, if plants succeed in promptly recolonising the burnt area, the pre-fire level of most properties can be recovered and even enhanced. This work is a review of the up-to-date literature dealing with changes imposed by fires on properties of forest soils. Ecological implications of these changes are described.", "keywords": ["Nitrogen", "Phosphorus", "Fire", " Forest ecosystems", " Forest soils", " Soil ecology", " Soil properties.", "04 agricultural and veterinary sciences", "15. Life on land", "Invertebrates", "01 natural sciences", "Carbon", "Fires", "Trees", "Soil", "13. Climate action", "Animals", "0401 agriculture", " forestry", " and fisheries", "Hydrophobic and Hydrophilic Interactions", "Soil Microbiology", "0105 earth and related environmental sciences"], "contacts": [{"organization": "CERTINI, GIACOMO", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s00442-004-1788-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-004-1788-8", "name": "item", "description": "10.1007/s00442-004-1788-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-004-1788-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-02-02T00:00:00Z"}}, {"id": "10.1007/s00442-005-0191-4", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:31Z", "type": "Journal Article", "created": "2005-07-22", "title": "Fine Root Chemistry And Decomposition In Model Communities Of North-Temperate Tree Species Show Little Response To Elevated Atmospheric Co2 And Varying Soil Resource Availability", "description": "Rising atmospheric [CO2] has the potential to alter soil carbon (C) cycling by increasing the content of recalcitrant constituents in plant litter, thereby decreasing rates of decomposition. Because fine root turnover constitutes a large fraction of annual NPP, changes in fine root decomposition are especially important. These responses will likely be affected by soil resource availability and the life history characteristics of the dominant tree species. We evaluated the effects of elevated atmospheric [CO2] and soil resource availability on the production and chemistry, mycorrhizal colonization, and decomposition of fine roots in an early- and late-successional tree species that are economically and ecologically important in north temperate forests. Open-top chambers were used to expose young trembling aspen (Populus tremuloides) and sugar maple (Acer saccharum) trees to ambient (36 Pa) and elevated (56 Pa) atmospheric CO2. Soil resource availability was composed of two treatments that bracketed the range found in the Upper Lake States, USA. After 2.5 years of growth, sugar maple had greater fine root standing crop due to relatively greater allocation to fine roots (30% of total root biomass) relative to aspen (7% total root biomass). Relative to the low soil resources treatment, aspen fine root biomass increased 76% with increased soil resource availability, but only under elevated [CO2]. Sugar maple fine root biomass increased 26% with increased soil resource availability (relative to the low soil resources treatment), and showed little response to elevated [CO2]. Concentrations of N and soluble phenolics, and C/N ratio in roots were similar for the two species, but aspen had slightly higher lignin and lower condensed tannins contents compared to sugar maple. As predicted by source-sink models of carbon allocation, pooled constituents (C/N ratio, soluble phenolics) increased in response to increased relative carbon availability (elevated [CO2]/low soil resource availability), however, biosynthetically distinct compounds (lignin, starch, condensed tannins) did not always respond as predicted. We found that mycorrhizal colonization of fine roots was not strongly affected by atmospheric [CO2] or soil resource availability, as indicated by root ergosterol contents. Overall, absolute changes in root chemical composition in response to increases in C and soil resource availability were small and had no effect on soil fungal biomass or specific rates of fine root decomposition. We conclude that root contributions to soil carbon cycling will mainly be influenced by fine root production and turnover responses to rising atmospheric [CO2], rather than changes in substrate chemistry.", "keywords": ["0106 biological sciences", "Science", "Climate", "Ecology and Evolutionary Biology", "Plant Roots", "01 natural sciences", "Trees", "Sugar Maple", "Soil", "Ergosterol", "Health Sciences", "Carbon-based Secondary Compounds", "Biomass", "Cellular and Developmental Biology", "Ecosystem", "Soil Microbiology", "Atmosphere", "Fungi", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Soil C Cycling", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Trembling Aspen"]}, "links": [{"href": "https://doi.org/10.1007/s00442-005-0191-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-005-0191-4", "name": "item", "description": "10.1007/s00442-005-0191-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-005-0191-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-07-23T00:00:00Z"}}, {"id": "10.1007/s00442-005-0249-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:31Z", "type": "Journal Article", "created": "2005-10-04", "title": "Fungal Community Composition And Metabolism Under Elevated Co2 And O-3", "description": "Atmospheric CO(2) and O(3) concentrations are increasing due to human activity and both trace gases have the potential to alter C cycling in forest ecosystems. Because soil microorganisms depend on plant litter as a source of energy for metabolism, changes in the amount or the biochemistry of plant litter produced under elevated CO(2) and O(3) could alter microbial community function and composition. Previously, we have observed that elevated CO(2) increased the microbial metabolism of cellulose and chitin, whereas elevated O(3) dampened this response. We hypothesized that this change in metabolism under CO(2) and O(3) enrichment would be accompanied by a concomitant change in fungal community composition. We tested our hypothesis at the free-air CO(2) and O(3) enrichment (FACE) experiment at Rhinelander, Wisconsin, in which Populus tremuloides, Betula papyrifera, and Acer saccharum were grown under factorial CO(2) and O(3) treatments. We employed extracellular enzyme analysis to assay microbial metabolism, phospholipid fatty acid (PLFA) analysis to determine changes in microbial community composition, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to analyze the fungal community composition. The activities of 1,4-beta-glucosidase (+37%) and 1,4,-beta-N-acetylglucosaminidase (+84%) were significantly increased under elevated CO(2), whereas 1,4-beta-glucosidase activity (-25%) was significantly suppressed by elevated O(3). There was no significant main effect of elevated CO(2) or O(3) on fungal relative abundance, as measured by PLFA. We identified 39 fungal taxonomic units from soil using DGGE, and found that O(3) enrichment significantly altered fungal community composition. We conclude that fungal metabolism is altered under elevated CO(2) and O(3), and that there was a concomitant change in fungal community composition under elevated O(3). Thus, changes in plant inputs to soil under elevated CO(2) and O(3) can propagate through the microbial food web to alter the cycling of C in soil.", "keywords": ["0106 biological sciences", "Food Chain", "Extracellular Enzymes", "Science", "Ecology and Evolutionary Biology", "Polymerase Chain Reaction\u2013Denaturing Gradient Gel Electrophoresis", "Polymerase Chain Reaction", "01 natural sciences", "Soil Microbial Community", "Soil", "Ozone", "Health Sciences", "Acetylglucosaminidase", "Cellular and Developmental Biology", "Ecosystem", "Soil Microbiology", "beta-Glucosidase", "Fatty Acids", "Fungi", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Fungal Metabolism", "Carbon", "Free-air CO 2 and O 3 Enrichment", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Extracellular Space"]}, "links": [{"href": "https://doi.org/10.1007/s00442-005-0249-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-005-0249-3", "name": "item", "description": "10.1007/s00442-005-0249-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-005-0249-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-10-05T00:00:00Z"}}, {"id": "10.1007/s00442-006-0562-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:32Z", "type": "Journal Article", "created": "2006-10-10", "title": "Is Microbial Community Composition In Boreal Forest Soils Determined By Ph, C-To-N Ratio, The Trees, Or All Three?", "description": "In Fennoscandian boreal forests, soil pH and N supply generally increase downhill as a result of water transport of base cations and N, respectively. Simultaneously, forest productivity increases, the understory changes from ericaceous dwarf shrubs to tall herbs; in the soil, fungi decrease whereas bacteria increase. The composition of the soil microbial community is mainly thought to be controlled by the pH and C-to-N ratio of the substrate. However, the latter also determines the N supply to plants, the plant community composition, and should also affect plant allocation of C below ground to roots and a major functional group of microbes, mycorrhizal fungi. We used phospholipid fatty acids (PLFAs) to analyze the potential importance of mycorrhizal fungi by comparing the microbial community composition in a tree-girdling experiment, where tree belowground C allocation was terminated, and in a long-term (34 years) N loading experiment, with the shifts across a natural pH and N supply gradient. Both tree girdling and N loading caused a decline of ca. 45% of the fungal biomarker PLFA 18:2omega6,9, suggesting a common mechanism, i.e., that N loading caused a decrease in the C supply to ectomycorrhizal fungi just as tree girdling did. The total abundance of bacterial PLFAs did not respond to tree girdling or to N loading, in which cases the pH (of the mor layer) did not change appreciably, but bacterial PLFAs increased considerably when pH increased across the natural gradient. Fungal biomass was high only in acid soil (pH < 4.1) with a high C-to-N ratio (>38). According to a principal component analysis, the soil C-to-N ratio was as good as predictor of microbial community structure as pH. Our study thus indicated the soil C-to-N ratio, and the response of trees to this ratio, as important factors that together with soil pH influence soil microbial community composition.", "keywords": ["Sweden", "Soil", "Bacteria", "Nitrogen", "Mycorrhizae", "Fatty Acids", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "15. Life on land", "Carbon", "Soil Microbiology", "Trees"]}, "links": [{"href": "https://doi.org/10.1007/s00442-006-0562-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-006-0562-5", "name": "item", "description": "10.1007/s00442-006-0562-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-006-0562-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-10-11T00:00:00Z"}}, {"id": "10.1007/s00442-009-1490-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:32Z", "type": "Journal Article", "created": "2010-03-06", "title": "Frequent Fire Affects Soil Nitrogen And Carbon In An African Savanna By Changing Woody Cover", "description": "When tropical and sub-tropical ecosystems burn, considerable amounts of N present in the biomass fuel may be released. This ultimately results in a loss of fixed N to the atmosphere. It is often assumed that this volatilization loss of N with frequent fire will result in a reduction of plant-available N and total system N. By changing the amount of woody biomass fire may, however, also have indirect effects on N and C dynamics. Here we consider the effects of 50 years of frequent fire on total soil N and soil organic C (SOC) and total soil N in a mesic savanna in the Kruger National Park, South Africa. We also determine how changes in woody biomass may affect total soil N and SOC. We measured soil and fine root N and C concentrations as well as total soil N and SOC pools in four burning treatments, including fire exclusion, of a long-term fire experiment. Our results show that regardless of soil depth, fire treatment had no significant effect on total soil N and SOC. Our results also show that under trees total soil N and SOC concentrations of the surface soil increase, and pools of N and SOC increase to a depth of 7 cm. However, the extent to which soil N and C dynamics differed under canopies and away from canopies was dependent on fire treatment. Our results show that the effect of fire on soil N and C is mediated both through the indirect effect of changes in woody cover and the direct effects of fire (volatilization losses of nutrients). We suggest that woody thickening in this mesic savanna will have pronounced effects on long-term N and C dynamics.", "keywords": ["0106 biological sciences", "Carbon Isotopes", "Time Factors", "Nitrogen Isotopes", "Nitrogen", "Rain", "04 agricultural and veterinary sciences", "15. Life on land", "Wood", "01 natural sciences", "Carbon", "Fires", "Trees", "Soil", "South Africa", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Seasons", "Volatilization", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00442-009-1490-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-009-1490-y", "name": "item", "description": "10.1007/s00442-009-1490-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-009-1490-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-03-07T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=soil+microbiology&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=soil+microbiology&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=soil+microbiology&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=soil+microbiology&offset=50", "hreflang": "en-US"}], "numberMatched": 554, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-05-25T10:58:38.389925Z"}